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1 경락경혈학회지 Vol.27, No.1, pp , 2010 Journal of Meridian & Acupoint Dept. of 1 Meridian & Acupoint, 3 Acupuncture & Moxibustion, College of Oriental Medicine, Daejeon University 2 Division of Clinical Medicine, School of Korean Medicine, Pusan National University Abstract Objective & Methods: The purpose of this study is to investigate the anti-oxidative and immuneregulative effects of electro-acupouncture(ea) at SP6(Sameumgyo) in aged rats. The author performed several experimental items including blood cell counts, blood chemistry, measurement of various oxidants and antioxidants in liver and spleen, analysis of various cytokines in spleen. The results are as follows. Results: 1. EA at SP6 significantly reduced the number of platelets in blood. 2. EA at SP6 significantly reduced NO concentration and significantly increased catalase activity in liver. 3. EA at SP6 significantly reduced NO concentration and significantly increased SOD activity, catalase activity and glutathione concentration in spleen. 4. EA at SP6 restored the increase of IL-4, IL-6 and the decrease of IFN- in aged rat spleen. Conclusion: According to these results, it is postulated that EA at SP6 has an antioxidative effect through increasing the activities of antioxidative enzymes and inhibiting production of oxidized substances, as well as an immune regulative effect in aging process. In consequence, it is presumed that EA at SP6 may have an anti-aging effect. Key words: Sameumgyo(SP6), electro-acupuncture, anti-aging, antioxidation, immune regulation

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4 Scheme I. Preparation of mitochondrial, microsomal and cytosolic fractions

5 γ Table 1. Effect of EA at SP6 on CBC(complete blood count) in rats

6 Fig. 1. Effect of EA at SP6 on the number of RBC in rat blood. Fig. 2. Effect of EA at SP6 on the number of W BC in rat blood. Fig. 3. Effect of EA at SP6 on the number of PLT in rat blood. Table 2. Effect of EA at SP6 on various parameters in serum of rats

7 Fig. 4. Effect of EA at SP6 on serum albumin in rats. Fig. 6. Effect of EA at SP6 on serum LDL cholesterol in rats. Fig. 5. Effect of EA at SP6 on serum total bilirubin in rats. Fig. 7. Effect of EA at SP6 on serum glucose in rats

8 Fig. 8. Effect of EA at SP6 on serum ALP in rats. Fig. 9. Effect of EA at SP6 on serum AST in rats. Fig. 10. Effect of EA at SP6 on serum ALT in rats. Table 3. Effect of EA at SP6 on various oxidants and antioxidants in rat liver

9 Fig. 11. Effect of EA at SP6 on liver SOD in rat liver. Fig. 13. Effect of EA at SP6 on NO level in rat liver. Fig. 12. Effect of EA at SP6 on glutathione level in rat liver. Fig. 14. Effect of EA at SP6 on M DA level in rat liver

10 Fig. 15. Effect of EA at SP6 on catalase activity in rat liver. Table 4. Effect of EA at SP6 on various oxidants and antioxidants in rat spleen Fig. 16. Effect of EA at SP6 on SOD activity in rat spleen. Fig. 17. Effect of EA at SP6 on glutathione level in rat spleen

11 Fig. 18. Effect of EA at SP6 on NO level in rat spleen. Fig. 19. Effect of EA at SP6 on M DA level in rat spleen. Fig. 20. Effect of EA at SP6 on catalase activity in rat spleen. Table 5. Effect of EA at SP6 on the levels of various cytokines in rat spleen γ

12 Fig. 21. Effect of EA at SP6 on IL-4 in rat spleen cell culture. Fig. 22. Effect of EA at SP6 on IL-6 in rat spleen cell culture. Fig. 23. Effect of EA at SP6 on IFN- in rat spleen cell culture

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