Development of culture technic for practical cultivation under structure in Gastrodia elate Blume

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1 Development of culture technic for practical cultivation under structure in Gastrodia elate Blume

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7 SUMMARY 1. Quality and yield of rhizomorph of Gastrodia by culture condition and method. Yield of Gastrodia by culture in P.E. film and non-wovcn fabric house was 12.3kg per 3.3 (3,690kg/10a) and it was incrcased more 189% than yield of Gastrodia, 6.5kg per 3.3 by culture in open field becasue of proper conservation of soil moisture and supperssion of weed germination by culture of P.E. film and non-woven fabric house. Highest yield of Gastrodia in house culture by fresh oak tree planted in spring was 22kg per 3.3 in Buhang and it`s result was due to prolong the growth pcriod of Gastrodia for 2~3 months. Yield of Gastrodia in tunnel culture was 7.9kg per 3.3 and it was more 122% than it of open field and highest yicld in Buhang was 11kg per 3.3 by culture of fresh oak tree planted in spring. Highest yield in open field during growth period of 2 years in Gusung was 9kg per 3.3 by culture of fresh oak tree planted in spring. 2. Commerial rhizomorph rate of Gastrodia by culture condition and regions. Growth of Gastrodia was effected by difference of soil condition rather than it of rcgion. Commerical rhizomorph rate of Gastrodia produced in culture of P.E. film house was 61% but it of open field was 46.2%. 3. Rooting of hypha by difference of culture material. Rooting of hypha was 100% in advanced oak tree but 90% in fresh oak tree and tresh oak tree + advanced cak tree. Hypha rooting of acacia tree was 30% but it of acacia + oak tree 70% and hypha was rooted more in oak tree than in acacia. Rooting and growth of hypha was good in advanced oak tree rather than fresh oak tree but it was good in fresh oak tree rather than advanced oak tree after 2 years. Period using fresh oak tree for culture of Gastrodia was long more 1~2 years than advanced oak tree and the culture of frech oak tree was economy rather than advanced oak tree because of material price and using period. Acacia tree had not produced rhizomorph of Gastrodia and acacia tree + oak tree produced only rhizomorph of 4.2g per material tree of 60cm

8 4. Propagation of rhiromorph in gastrodia. Suitable amount of spawn and radicle for propagation was 40 bottle of spawn and 6kg of radicle per 3.3 because hypha of Gastrodia could grown by nutrition of spawn rather than it of advanced oak tree. 5. Economy analysis. Cost of dry production of Gastrodia was higer 250% than that of tresh production. Real income by house culture was 8,870,000 won per 330 and it of culture by tunnel and open field was each 5,830,000 won and 4,295,000 won. Rate of income in culture by house, tunnel and open field was cach 73%, 71% and 66%

9 CONTENTS CH. 1. INTRODUCTION CH. 2. EXPERIMENTAL PERFORMANCE Materials and methods ) innoculation of spawn in Gastrodia ) planting of oak tree ) plot design of acacia tree ) propagation of rhizomorph in Gastrodia ) culture structure and condition Arrangement ) plot design of experiment by difference of culture form and oak tree ) plot design of rhizomorph for propagation CH. 3 RESULTS AND DISCUSSION Inverstigation of air temperature and soil analysis in experimental regions Yield and quality of rhizomorph in house, tunnel and open field ) yield of rhizomorph by form of culture structure ) yield and size of rhizomorph by culture structure and region Rooting of hypha by culture medium as advance oak tree, fresh oak tree and advance + fresh oak tree Production of rhizomorph for propagation Economical analysis Conclusion Future project REFERENCES

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12 6~7 ( )( ) ( ) 10~30cm 2. 10~50cm 7~12m, 2mm. ( ) ( ). ( ) ( ) ( ) ( ). ( ) 20~2540~45% ( ) ( )( ) 4~5%( ) ( ) ( )( ). ( ), ( ) ( ), ( ) ( ) ( ) ( ) ( ) ( ) ( ) ( ) ( )( ) ( ) ( )( ) ( ) ( )

13 ( ) ( ) 200m ~ 500m ( ) ( ) ( ) ( )( ), 3300 ( 100, 50, 150) ( ) ( )( ) ( ) ( ) 2 ~3 ( ) ( ) ( ) ( ). ( )( ) ( ) 15cm 60cm ( )( ) ( ) ( ) ( ). [ 1] " ", +, +,, +, +,, +, +, [ 1]

14 . 2 ~ 3cm, ( ) 1cm10cm 60cm 20 ~ 25 1g ( ). [ 1]. ( ) ( ) 20 ~ 30cm 5cm 20cm ( )10,,, ( ) (50%)

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18 . 1) ~ mm 1.5T 9m 0.1mm 1,. 3 m. [ 3] 40 m 6.5m 2.5m 6.5 m

19 2) 210cm ( ) 50cm ( ) (50%) ( ). 65m 1.2m 50cm 1.2m 0.5m

20 2.. ( 100 ) + (5 ) (30 ) (30 ) (30 ) + (5 ) ( 50 ) + (2.5 ) (15 ) (15 ) (15 ) + (2.5 ) ( 150 ) + (7.5 ) (45 ) (45 ) (45 ) + (7.5 )

21 . [ 3] 12 (3 4) 12 (3 4) 12 (3 4) ) [ 4]

22 [ 6] [ 7]

23 2) [ 5]

24 [ 8] [ 9]

25 [ 10] [ 11]

26 3 1., ph, ( ) ( ), ( ), ( ),, ( ), ( ) ( ). [ 4] ph (%) (ppm) (me/ 100g) (me/ 100g) (kg/10a) EC (ms/ cm) " " [ 6] (' ~ ) ( ) (%). ( 200m) ( 400m) ( 500m)

27 2.,,. [ 6] (2 ) (kg/ )... A/C A/C A/C A/C (A) (B) (C)

28 ( )( ) 12.3kg/ (3,690kg/10a)( ) 6.5kg/ (1,950kg/10a) 189% ( ) 22kg/ (6,600kg/10a). 7.9kg(2,370kg/10a) 122% ( ) ( ). 11kg/ (3,300kg/10a). 9kg/ (2,700kg/10a). ( ), ( ) ( ), (2 ~ 3 )( ) ( ) ( ). 2 3 ( ) [ 10]

29 [ 11] [ 12]

30 .,, [ 7],, (kg/. 2 ) ( ) 39%, 61% 45.6%, 54.4% ( ) 53.8%, 46.2% ( )

31 3.,, +.,, % - 90 % - 95 % 100 % 100 % 100 %., % - 70 % 100 % 100 % % % 100 % 100 %,, + ( ) 90 % ( ) 100% ( )., + ( )30% ( ) + 70%. 2 ( ). 4 ~ 5 3 ~ 4 ( ) ( )

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33 . (1 ) (g/ ) " " " " " " ( ) 1 140g/. ( ), g/ ( ) ( ) ( ) [ 15]

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35 4.. + (6 ) ( ) ( ) ( ) ( ) kg/ kg/. [ 8] (kg/ ) ( ) ( ) ( ) / ( )

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37 5. (100 /2 ) ( ) ( ) ( ) ( ) ( ) (%) ,000 12,240 3,370 8, " 8,205 2,375 5, " 6,540 2,245 4, ( ) 30% 15,000 / 1, % ( ). ( )8,870 /100, 5,830, 4,295 ( ) 207%, 136%, 73%, 71%, 66%. [

38 [ 21] [ 22]

39 kg/ (3,690kg/10a) 6.5kg/ (1,950kg/ 10a 189% ( ) 22kg/ (6,600kg/10a), (2 ~ 3 ) ( ). ( ) 7.9kg(2,370kg/10a) 122% ( ). 11kg/ (3,300kg/10a). 9kg/ (2,700kg/10a). 2 3 ( ) ( ), ( ) ( ) ( ) ( ) ( ),..,, ( ) ( ) - 39%, 61% %, 46.2%.,, +,, + ( ) 90 % ( ) 100% ( )., + ( )30% + 70%

40 1 ( ) 2. 4 ~ 5 3 ~ 4 ( ) ( ) ( ) g/. ( ), + ( ) 42.7g/ ( ) kg/ kg/ / ( ) ( ).. ( ) 15,000 / 1,800( ) 250% ( ). ( )8,870 /100, 5,830, 4, %, 136%, ( ) 73%, 71%, 66%

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44 ( ) ( ) Development of culture technic for practical cultivation under structure in Gastrodia elate Blume ( ) ( ) 60, ~ , ( 6 ) 9 ( ) ( 3 ) (1),, (2),, + (3) (4), + (5) (1),, 12.3kg / (3,690kg /10a) 6.5kg/ (1,950kg/ 10a 189% 22kg / (6,600kg/ 10a), (2 ~ 3 ). 7.9kg(2,370kg/10a) 122%. 11kg/ (3,300kg/10a). 9kg / (2,700kg/ 10a). 2 3,,. (2),, - 39%, 61% %, 46.2%

45 (3),, +,, + 90 % 100%., + ( )30% + 70% ~ 5 3 ~ g/., g / (4) kg/ kg / /. (5) 15,000 / 1, %. 8,870 /100, 5,830, 4, %, 136%, 73%, 71%, 66%. ( ) (1) (2) (3)

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