3 Development of Long-term Storage Method in Fruit Nursery Stock

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1 3 Development of Long-term Storage Method in Fruit Nursery Stock

2 : : :

3 . (Development of Long-term Storage Method in Fruit Nursery Stock). 1. O O O (5 8 ) 2.,. 1,. 3-4 (5-8 ) - 1 -

4 , 1., , 2 ( ), ( ), ( ) ,, -5, 0, 5, - 2 -

5 ,,.. 2, 3 ( ), ( ), ( ) ,, -5, 0, 5,,,. 2. PE film, PE film, Wax PE film.. 1, 2 ( ), ( ), ( ) PE film, PE film, wax PE film,,,.. 2, 3 ( ), ( ), ( )

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11 SUMMARY Difficulty in estimating the demands for fruit tree nursery stocks repeatedly has resulted in their over-production or shortage. The surplus nursery stocks due to over-production, in particular, have caused nursery stock producers serious to suffer serious economic losses. This study, therefore, was carried out to develop storage method for the surplus nursery stocks and thereby to construct an on-demand supply system in nursery stocks. To determine the optimal storage temperature, nursery stocks of 6 fruit trees were stored at -5, 0 and 5 before planting in May, June, July, and August. Their growth patterns were measured in October and summarized as follows; 1. Apple 'Fuji' nursery stock stored at 0 showed the highest survival rate and growth of nursery stock after planting, but at 5 storage, its survival rate was less than 20%. 2. For Japanese pear 'Niitaka', the survival rate and growth of nursery stock after planting were higher at 0 and 5 storage than at -5 storage. 3. The survival rate and growth after planting in grape 'Sheridan' nursery stock appeared to be higher at 0 storage than at 5 and -5 storage. 4. The survival rate and growth after planting in peach 'Yumyoung' nursery stock were the highest at 0 storage. The storage at 5 caused a part of nursery stocks to budbreak during storage. 5. The storage at 0 and 5 had higher survival rate and growth after - 9 -

12 planting in persimmon 'Fuyu' nursery stock than did the storage at -5. The budbreak of nursery stock during storage was not found at 5 storage unlike the peach nursery stock. 6. For kiwifruit 'Hayward' nursery stock, the storage temperature appeared to be 0 because of the high survival rate and growth. To develop the optimal packing method, the nursery stocks of 6 fruit trees were packed with 3 different methods: polyethylene film bag packing (PE); polyethylene film bag packing after spraying nursery stocks with benlate (PE + Benlate); polyethylene film bag packing after spraying nursery stocks with wax (PE + Wax). After storing at 0 until April, the packed nursery stocks were planted and their survival rate and growth were measured in October. The result were summarized as follows; 1. In apple 'Fuji', the spray of benlate and wax increased the survival rate of nursery stock after planting in comparison with PE, but had no effect on the number of newly developed shoot. The spray of benlate appeared to be effective on the growth of nursery stock after planting. 2. In Japanese pear 'Niitaka', the survival rate and growth of nursery stock after planting were increased with the spray of either benlate or wax. In addition, the spray of benlate was closely related to the increased number of the newly developed shoot. 3. In grape 'Sheridan', the wax spray increased the survival rate of nursery stocks after planting compared with PE, whereas the benlate spray decreased

13 the survival rate and growth. 4. In peach 'Yumyoung', the benlate spray showed the best growth of nursery stock after plating of all packing methods with considerably high survival rate and growth. 5. In persimmon 'Fuyu', all packing methods including PE had the considerable survival rate and growth of nursery stocks after planting. The benlate spray increased somewhat the growth of them. 6. In kiwifruit 'Hayward', PE showed the highest survival rate and growth of nursery stocks after planting. To develop the supplemental planting method during growing season, the nursery stocks of 6 fruit trees were stored at 0 from March for planting in Apri, May, June, July and August, respectively. The nursery stocks planted after stepwise heating were measured on survival rate and growth. The obtained results were summarized as follows; 1. In apple 'Fuji', the survival rate and growth of nursery stock after planting decreased with delay of planting time. In particular, the planting without heating in July showed the lowest survival rate (<20%). Heating treatment had no effect on the growth after planting. 2. In Japanese pear 'Niitaka', all planting times showed the high survival rate of nursery stock after planting (>80%), without differences among planting times. The growth after planting appeared to be higher with the planting in June than in April, May and August. 3. In grape 'Sheridan', the planting in April, May and June showed about 65%

14 of the survival rate of nursery stock after planting, but planting in July and August below 20%. The growth after planting was also related with planting time in a same manner. 4. In peach 'Yumyoung', the survival rate of nursery stocks after planting appeared to be considerably high in all planting times and heating treatment except for the planting in July without heating and the planting in August with stepwise heating treatment. The planting after July showed unfavorable growth after planting. 5. In persimmon 'Fuyu', the highest survival rate and growth of nursery stocks after planting were ontained with the planting in June without heating and with stepwise heating. 6. In kiwifruit 'Hayward', the planting in May and June with stepwise heating showed good survival rate and growth of nursery stocks after planting. The stepwise heating caused the nursery stocks to budbreak during the heating treatment

15 CONTENTS 1. Introduction Objective and Range Necessity of development Performance Establishment of optimum storage temperature of fruit seedlings Introduction Materials and Method Result Performance Development of storage methods of fruit seedlings Introduction Materials and Method Result Performance

16 4. Development of supplemental planting method of fruit seedlings at growth seasons Introduction Materials and Method Result Performance Summary in Korean References Appendix

17

18

19 List of Tables Table 1. Production and supply of fruit nursery stock yearly Table 2. Mineral and carbohydrate content in stem and root of 'Fuji' apple fruit nursery stock to different storage temperature after storage Table 3. Mineral and carbohydrate content in stem and root of 'Niitaka' pear fruit nursery stock to different storage temperature after storage Table 4. Mineral and carbohydrate content in stem and root of 'Sheridan' grape fruit nursery stock to different storage temperature after storage Table 5. Mineral and carbohydrate content in stem and root of 'Yumyoung' peach fruit nursery stock to different storage temperature after storage Table 6. Mineral and carbohydrate content in stem and root of 'Fuyu' persimmon fruit nursery stock to different storage temperature after storage Table 7. Mineral and carbohydrate content in stem and root of 'Hayward' kiwifruit nursery stock to different storage temperature after storage Table 8. Mineral and carbohydrate content in stem of 'Fuji' apple fruit nursery stock to different storage methods after storage Table 9. Mineral and carbohydrate content in stem of 'Niitaka' pear fruit nursery stock to different storage methods after storage Table 10. Mineral and carbohydrate content in stem of 'Sheridan' grape fruit nursery stock to different storage methods after storage Table 11. Mineral and carbohydrate content in stem of 'Yumyoung' peach fruit nursery stock to different storage methods after storage Table 12. Mineral and carbohydrate content in stem of 'Fuyu' persimmon fruit

20 nursery stock to different storage methods after storage Table 13. Mineral and carbohydrate content in stem of 'Hayward' kiwifruit nursery stock to different storage methods after storage Table 14. Mineral and carbohydrate content in stem of apple 'Fuji' nursery stock by supplemental planting method Table 15. Mineral and carbohydrate content in stem of pear 'Niitaka' nursery stock by supplemental planting method Table 16. Mineral and carbohydrate content in stem of grape 'Sheridan' nursery stock by supplemental planting method Table 17. Mineral and carbohydrate content in stem of peach 'Yumyoung' nursery stock by supplemental planting method Table 18. Mineral and carbohydrate content in stem of persimmon 'Fuyu' nursery stock by supplemental planting method Table 19. Mineral and carbohydrate content in stem of kiwifruit 'Hayward' nursery stock by supplemental planting method

21 List of Figures Fig. 1. Survival rate of apple 'Fuji' nursery stock by storage temperature Fig. 2. Shoot length and shoot numbers of apple 'Fuji' nursery stock by storage temperature Fig. 3. Survival rate of pear 'Niitaka' nursery stock by storage temperature Fig. 4. Shoot length and shoot numbers of pear 'Niitaka' nursery stock by storage temperature Fig. 5. Survival rate of grape 'Sheridan' nursery stock by storage temperature Fig. 6. Shoot length and shoot numbers of grape 'Sheridan' nursery stock by storage temperature Fig. 7. Survival rate of peach 'Yumyoung' nursery stock by storage temperature Fig. 8. Shoot length and shoot numbers of peach 'Yumyoung' nursery stock by storage temperature Fig. 9. Survival rate of persimmon 'Fuyu' nursery stock by storage temperature Fig. 10. Shoot length and shoot numbers of persimmon 'Fuyu' nursery stock by storage temperature Fig. 11. Survival rate of kiwifruit 'Hayward' nursery stock by storage temperature. 47 Fig. 12. Shoot length and shoot numbers of kiwifruit 'Hayward' nursery stock by storage temperature Fig. 13. Survival rate of apple 'Fuji' nursery stock by storage method Fig. 14. Shoot length and shoot numbers of apple 'Fuji' nursery stock by storage method Fig. 15. Survival rate of pear 'Niitaka' nursery stock by storage method Fig. 16. Shoot length and shoot numbers of pear 'Niitaka' nursery stock

22 by storage method Fig. 17. Survival rate of grape 'Sheridan' nursery stock by storage method Fig. 18. Shoot length and shoot numbers of grape 'Sheridan' nursery stock by storage method Fig. 19. Survival rate of peach 'Yumyoung' nursery stock by storage method Fig. 20. Shoot length and shoot numbers of peach 'Yumyoung' nursery stock by storage method Fig. 21. Survival rate of persimmon 'Fuyu' nursery stock by storage method Fig. 22. Shoot length and shoot numbers of persimmon 'Fuyu' nursery stock by storage method Fig. 23. Survival rate of kiwifruit 'Hayward' nursery stock by storage method Fig. 24. Shoot length and shoot numbers of kiwifruit 'Hayward' nursery stock by storage method Fig. 25. Survival rate of apple 'Fuji' nursery stock by supplemental planting method Fig. 26. Final growth of apple 'Fuji' nursery stock by supplemental planting method Fig. 27. Final shoot number of apple 'Fuji' nursery stock by supplemental planting method Fig. 28. Survival rate of pear 'Niitaka' nursery stock by supplemental planting method Fig. 29. Final growth of pear 'Niitaka' nursery stock by supplemental planting method Fig. 30. Final shoot number of pear 'Niitaka' nursery stock by supplemental planting method

23 Fig. 31. Survival rate of grape 'Sheridan' nursery stock by supplemental planting method Fig. 32. Final growth of grape 'Sheridan' nursery stock by supplemental planting method Fig. 33. Final shoot number of grape 'Sheridan' nursery stock by supplemental planting method Fig. 34. Survival rate of peach 'Yumyoung' nursery stock by supplemental planting method Fig. 35. Final growth of peach 'Yumyoung' nursery stock by supplemental planting method Fig. 36. Final shoot number of peach 'Yumyoung' nursery stock by supplemental planting method Fig. 37. Survival rate of persimmon 'Fuyu' nursery stock by supplemental planting method Fig. 38. Final growth of persimmon 'Fuyu' nursery stock by supplemental planting method Fig. 39. Final shoot number of persimmon 'Fuyu' nursery stock by supplemental planting method Fig. 40. Survival rate of kiwifruit 'Hayward' nursery stock by supplemental planting method Fig. 41. Final growth of kiwifruit 'Hayward' nursery stock by supplemental planting method Fig. 42. Final shoot number of kiwifruit 'Hayward' nursery stock by supplemental planting method

24 1 1. WTO.. 1,. (5-8 ) 10%. 1.,,

25 O. O UR,. O O 1,. Table 1. Production and supply of fruit nursery stock yearly. <Unit:Trees> Production 6,439,000 6,040,400 6,142,400 6,531,200 Supply 5,506,000 4,097,400 4,086,500 5,700,000 Surplus (%) 933,000 (14.5) 1,943,000 (32.2) 2,055,900 (33.5) 831,200 (12.7) Association of Korea Fruit nursery stock

26 O 3-4 (5-8 ), 1. O,,.. (1) O. O. O.. (2) O. O. O. (3) O (5 8 ). O

27 2.. O. O O. O 1. O 1. O.. O. O. O

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29 O. O. O (5-8 ).. O. O,. O 5 8,,

30 ( )..,

31 2 1.., ( ), ( ), ( ) 3, ( ), ( ), ( ) , 0, mm Polyethylene(PE) film

32 1 3 5, m.. 10,. Dry Oven,. N 500mg (98% H2SO4) 7mL (K2SO4 CuSO4 9:1g ) 5g Kjeldahl Automatic nitrogen analyzer(distillation unit Büchi 322, Control unit Büchi 342, Autotitrator E 526, Dosimat 666, Epson HX-20 ). N 500mg 100mL flask, ternary solution(hno3:h2so4:hclo4 = 10:1:4 volume ) 10mL P tube 5mL 30 incubator 15 (uv/vis Spectrophotometer, Gilford 260) 470nm OD. K, Ca, Mg (Atomic absorption spectrophotometer, Perkin Elmer 2380). 200mg 100mL flask 0.7N HCl 20mL mL fill-up 5mL 2.5mL % Anthrone 6mL,

33 10 (uv/vis Spectrophotometer, Gilford 260) 640nm OD ) % -5 70%, +5 50% (Fig. 1) ) 42.3cm, cm, +5-5 (Fig. 2). 3)

34 Fig. 1. Survival rate of apple 'Fuji' nursery stock by storage temperature

35 Fig. 2. Shoot length and shoot numbers of apple 'Fuji' nursery stock by storage temperature

36 (Fig. 2)., ), % -5 60% (Fig. 3). 2), +5 0 (Fig. 4). 3) (Fig. 4).,

37 Fig. 3. Survival rate of pear 'Niitaka' nursery stock by storage temperature

38 Fig. 4. Shoot length and shoot numbers of pear 'Niitaka' nursery stock by storage temperature

39 . 1) 0,. 100% % (Fig. 5). 2) 25cm, 0 30cm. +5 (Fig. 6). 3) (Fig. 6).,

40 Fig. 5. Survival rate of grape 'Sheridan' nursery stock by storage temperature

41 Fig. 6. Shoot length and shoot numbers of grape 'Sheridan' nursery stock by storage temperature

42 . 1) 0 100%., %, (Fig. 7). 2) 0 45cm cm (Fig. 8). 3) (Fig. 8).,

43 Fig. 7. Survival rate of peach 'Yumyoung' nursery stock by storage temperature

44 Fig. 8. Shoot length and shoot numbers of peach 'Yumyoung' nursery stock by storage temperature

45 . 1) % % (Fig. 9). 2), +5 0 (Fig. 10). 3) (Fig. 10).,

46 Fig. 9. Survival rate of persimmon 'Fuyu' nursery stock by storage temperature

47 Fig. 10. Shoot length and shoot numbers of persimmon 'Fuyu' nursery stock by storage temperature

48 . 1), 0. (Fig. 11). 2) (Fig. 12). 3) 1 0 (Fig. 12)., ,

49 Fig. 11. Survival rate of kiwifruit 'Hayward' nursery stock by storage temperature

50 Fig. 12. Shoot length and shoot numbers of kiwifruit 'Hayward' nursery stock by storage temperature

51 2.. 1.,,,,,. N, P, K, Ca, Mg, ,3,4,5,6,7, 6,,

52 Table 2. Mineral and carbohydrate content in stem and root of 'Fuji' apple fruit nursery stock to different storage temperature after storage. Treatment Mineral (%) N P K Ca Mg Carbohydrate (%) Stem Control 0.815az 0.103a 0.630a 0.733a 0.097a 8.5a b 0.080ab 0.532b 0.423bc 0.067b 6.4b b 0.074b 0.554b 0.554b 0.069b 6.1b c 0.054c 0.432c 0.378c 0.040c 4.4c Root Control 1.540a 0.200a 0.989a 0.233a 0.074a 10.5a b 0.151b 0.662b 0.152bc 0.050b 7.4b ab 0.143b 0.714b 0.171b 0.059b 7.7b c 0.101c 0.592c 0.137c 0.040c 5.9c z) Mean separation within columns by Duncan's multiple range test, 5% level

53 Table 3. Mineral and carbohydrate content in stem and root of 'Niitaka' pear fruit nursery stock to different storage temperature after storage. Treatment Mineral (%) N P K Ca Mg Carbohydrate (%) Stem Control 0.843az 0.173a 0.720a 0.735a 0.078a 9.5a b 0.158ab 0.512b 0.523b 0.069ab 7.1b b 0.137b 0.444bc 0.604ab 0.059b 6.1bc c 0.093c 0.392c 0.470c 0.042c 5.0c Root Control 1.152a 0.296a 1.261a 0.321a 0.062a 12.5a b 0.151b 0.662b 0.152bc 0.050b 7.4b ab 0.143b 0.714b 0.171b 0.059b 7.7b c 0.101c 0.592c 0.137c 0.040c 5.9c z) Mean separation within columns by Duncan's multiple range test, 5% level

54 Table 4. Mineral and carbohydrate content in stem and root of 'Sheridan' grape fruit nursery stock to different storage temperature after storage. Treatment Mineral (%) N P K Ca Mg Carbohydrate (%) Stem Control 0.755az 0.203a 0.620a 0.543a 0.100a 7.9a b 0.149b 0.532a 0.423b 0.077b 6.6b b 0.128bc 0.554a 0.490a 0.062b 6.0b c 0.100c 0.401b 0.298c 0.048c 5.4c Root Control 1.286a 0.578a 0.510a 0.253a 0.090a 9.9a b 0.430b 0.498a 0.229a 0.067b 8.0b b 0.408b 0.421b 0.199b 0.059b 7.5b c 0.294c 0.400b 0.154c 0.041c 6.1c z) Mean separation within columns by Duncan's multiple range test, 5% level

55 Table 5. Mineral and carbohydrate content in stem and root of 'Yumyoung' peach fruit nursery stock to different storage temperature after storage. Treatment Mineral (%) N P K Ca Mg Carbohydrate (%) Stem Control 1.245az 0.183a 0.590a 1.023a 0.137a 11.5a b 0.091b 0.375b 0.826b 0.077b 8.3b bc 0.094b 0.355b 0.932ab 0.062b 7.3bc c 0.073c 0.263c 0.550c 0.077b 6.4c Root Control 1.598a 0.279a 0.760a 0.803a 0.101a 17.2a bc 0.183b 0.700a 0.710ab 0.099a 12.1b b 0.177b 0.504b 0.723ab 0.100a 13.2b c 0.123c 0.495b 0.659b 0.072b 9.9c z) Mean separation within columns by Duncan's multiple range test, 5% level

56 Table 6. Mineral content and carbohydrate in stem and root of 'Fuyu' persimmon fruit nursery stock to different storage temperature after storage. Treatment Mineral (%) N P K Ca Mg Carbohydrate (%) Stem Control 1.524az 0.099a 0.830a 0.613a 0.062a 8.5a b 0.083ab 0.737ab 0.376b 0.059a 5.3b b 0.079b 0.797ab 0.409b 0.052a 5.1b c 0.625c 0.650b 0.388b 0.040b 3.4c Root Control 1.944a 0.180a 1.270a 0.579a 0.053a 10.4a b 0.175ab 0.990ab 0.376b 0.050a 8.8b bc 0.169ab 0.929ab 0.359b 0.052a 8.7b c 0.155b 0.810b 0.380b 0.030b 6.0c z) Mean separation within columns by Duncan's multiple range test, 5% level

57 Table 7. Mineral and carbohydrate content in stem and root of 'Hayward' kiwifruit nursery stock to different storage temperature after storage. Treatment Mineral (%) N P K Ca Mg Carbohydrate (%) Stem Control 0.585az 0.133a 0.440a 0.393a 0.082a 6.9a ab 0.110b 0.302b 0.266b 0.065b 5.4b b 0.109b 0.299b 0.250b 0.069b 5.3b c 0.099b 0.192c 0.180c 0.060b 3.9c Root Control 0.965a 0.283a 0.610a 0.323a 0.060a 8.3a ab 0.277a 0.570a 0.292a 0.055b 6.4b b 0.270a 0.588a 0.300a 0.050b 6.7b c 0.255b 0.310b 0.199b 0.039c 3.9c z) Mean separation within columns by Duncan's multiple range test, 5% level

58 4.,

59 ,,, 1, 2,, (1 : , 2 : ). 1,,,

60 1. 1) 0 (control) 2) PE film 0 3) (1000 ) + PE film 0 4) Wax + PE Film 0 2.,,, ), (control), 100% (Fig. 13). 2) (control) 40cm, PE+ 75cm PE+Wax PE (Fig. 14)

61 Fig. 13. Survival rate of apple 'Fuji' nursery stock by storage method

62 Fig. 14. Shoot length and shoot numbers of apple 'Fuji' nursery stock by storage method

63 3) (control) 4 6 (Fig. 14)., PE+.. 1) (control), 100% (Fig. 15). 2), (control) 20cm, 50cm (Fig. 16). 3), (control) PE film (Fig. 16).,,

64 Fig. 15. Survival rate of pear 'Niitaka' nursery stock by storage method

65 Fig. 16. Shoot length and shoot numbers of pear 'Niitaka' nursery stock by storage method

66 . 1) PE film Wax 70%, PE 40% (Fig. 17). 2) (control) 20cm, PE film 80cm PE+ 30cm PE+Wax 40cm (Fig. 18). 3) PE film 4 PE+Wax PE+ (Fig. 18). Control.,

67 Fig. 17. Survival rate of grape 'Sheridan' nursery stock by storage method

68 Fig. 18. Shoot length and shoot numbers of grape 'Sheridan' nursery stock by storage method

69 . 1), (Fig. 19). 2), (control) PE+ 70cm (Fig. 20). 3) 6 (Fig. 20).,,.. 1) (Fig. 21). 2) (Fig. 22)

70 Fig. 19. Survival rate of peach 'Yumyoung' nursery stock by storage method

71 Fig. 20. Shoot length and shoot numbers of peach 'Yumyoung' nursery stock by storage method

72 Fig. 21. Survival rate of persimmon 'Fuyu' nursery stock by storage method

73 Fig. 22. Shoot length and shoot numbers of persimmon 'Fuyu' nursery stock by storage method

74 3), PE film,. (Fig. 22).,, PE film.. 1), PE film Wax 80% (Fig. 23). 2) (control) 20cm, PE film 80cm PE+ 30cm PE+Wax 40cm. (Fig. 24)

75 Fig. 23. Survival rate of kiwifruit 'Hayward' nursery stock by storage method

76 Fig. 24. Shoot length and shoot numbers of kiwifruit 'Hayward' nursery stock by storage method

77 3) PE film 4 PE+Wax PE+ (Fig. 24).,,. 2. N, P, K, Ca, Mg (control), (Table 8),, wax (Table 9)., (control), (Table 10),, PE film (Table 11)., (control), (Table 12),, PE film Wax (Table 13)

78 Table 8. Mineral and carbohydrate content in stem of 'Fuji' apple fruit nursery stock to different storage methods after storage. Treatment Mineral (%) N P K Ca Mg Carbohydrate (%) Initialz 0.815ax 0.103a 0.630a 0.733a 0.097a 8.5a controly 0.546c 0.067c 0.487c 0.490c 0.045b 5.5c P.E. film 0.658b 0.070b 0.560b 0.623b 0.089a 7.0b P.E. film +Benlate 0.684b 0.086ab 0.554b 0.650b 0.090a 6.9b P.E.+Wax 0.690b 0.088ab 0.562b 0.663bc 0.090a 6.8b z) One year old nursery stock without storage y) nursery stock with storage at 0 x) Mean separation within columns by Duncan's multiple range test, 5% level

79 Table 9. Mineral and carbohydrate content in stem of 'Niitaka' pear fruit nursery stock to different storage methods after storage. Treatment Mineral (%) N P K Ca Mg Carbohydrate (%) Initialz 0.843ax 0.173a 0.720a 0.735a 0.078a 9.5a controly 0.588c 0.099c 0.412c 0.590b 0.069a 5.5c P.E. film 0.689b 0.121b 0.512b 0.537c 0.071a 7.8b P.E. film +Benlate 0.744ab 0.158ab 0.539b 0.612b 0.068a 8.7ab P.E.+Wax 0.701b 0.139b 0.520b 0.660ab 0.072a 8.0b z) One year old nursery stock without storage y) nursery stock with storage at 0 x) Mean separation within columns by Duncan's multiple range test, 5% level

80 Table 10. Mineral and carbohydrate content in stem of 'Sheridan' grape fruit nursery stock to different storage methods after storage. Treatment Mineral (%) N P K Ca Mg Carbohydrate (%) Initialz 0.755ax 0.203a 0.620a 0.543a 0.100a 7.9a controly 0.520c 0.108d 0.411c 0.319c 0.052c 5.6c P.E. film 0.670ab 0.159b 0.587ab 0.442b 0.073b 6.9ab P.E. film +Benlate 0.556b 0.130c 0.534b 0.450b 0.066b 6.0b P.E.+Wax 0.700a 0.170ab 0.560b 0.478b 0.062b 6.1b z) One year old nursery stock without storage y) nursery stock with storage at 0 x) Mean separation within columns by Duncan's multiple range test, 5% level

81 Table 11. Mineral and carbohydrate content in stem of 'Yumyoung' peach fruit nursery stock to different storage methods after storage. Treatment Mineral (%) N P K Ca Mg Carbohydrate (%) Initialz 1.245ax 0.183a 0.590a 1.023a 0.137a 11.5a controly 0.799d 0.073c 0.277c 0.570c 0.096b 7.0c P.E. film 0.820c 0.084bc 0.380b 0.826b 0.084b 8.3b P.E. film +Benlate 0.930b 0.102b 0.369b 0.941ab 0.070c 8.0b P.E.+Wax 0.812c 0.090b 0.360b 0.944ab 0.089b 7.3bc z) One year old nursery stock without storage y) nursery stock with storage at 0 x) Mean separation within columns by Duncan's multiple range test, 5% level

82 Table 12. Mineral and carbohydrate content in stem of 'Fuyu' persimmon fruit nursery stock to different storage methods after storage. Treatment Mineral (%) N P K Ca Mg Carbohydrate (%) Initialz 1.524ax 0.099a 0.830a 0.613a 0.062a 8.5a controly 0.701c 0.688c 0.700b 0.400b 0.040b 4.4c P.E. film 1.028b 0.080b 0.755ab 0.424b 0.059a 5.3b P.E. film +Benlate 0.989b 0.080b 0.801a 0.412b 0.052a 5.4b P.E.+Wax 1.020b 0.081b 0.790a 0.421b 0.053a 5.1b z) One year old nursery stock without storage y) nursery stock with storage at 0 x) Mean separation within columns by Duncan's multiple range test, 5% level

83 Table 13. Mineral and carbohydrate content in stem of 'Hayward' kiwifruit nursery stock to different storage methods after storage. Treatment Mineral (%) N P K Ca Mg Carbohydrate (%) Initialz 0.585ax 0.133a 0.440a 0.393a 0.082a 6.9a controly 0.312c 0.083d 0.221c 0.211c 0.051b 4.2d P.E. film 0.449a 0.121b 0.309b 0.266b 0.062b 5.5b P.E. film +Benlate 0.398b 0.109c 0.310b 0.220c 0.067b 4.8c P.E.+Wax 0.433a 0.123b 0.297b 0.242b 0.067b 5.3b z) One year old nursery stock without storage y) nursery stock with storage at 0 x) Mean separation within columns by Duncan's multiple range test, 5% level

84 4.,,

85 4 1. (5-8 ) 10% ,,, 1, 2,, , ,,,

86 . 1. 1) 4 (control) 2) ) ) ) ,,,

87 ,. ) ( ) (4 ) 5 6, %, %.,. (Fig. 25, 26, 27). ) ( ) 80%,. 6,7 5,8 (Fig. 28, 29, 30). ) ( ),, 5,6 65% 7,8 20%

88 Fig. 25. Survival rate of apple 'Fuji' nursery stock by supplemental planting method. Fruit nursery stock planted without warming treatment. Fruit nursery stock planted with intermittent warming treatment ( ). Fruit nursery stock planted with intermittent warming treatment ( )

89 Fig. 26. Final growth of apple 'Fuji' nursery stock by supplemental planting method. Fruit nursery stock planted without warming treatment. Fruit nursery stock planted with intermittent warming treatment ( ). Fruit nursery stock planted with intermittent warming treatment ( )

90 Fig. 27. Final shoot number of apple 'Fuji' nursery stock by supplemental planting method. Fruit nursery stock planted without warming treatment. Fruit nursery stock planted with intermittent warming treatment ( ). Fruit nursery stock planted with intermittent warming treatment ( )

91 Fig. 28. Survival rate of pear 'Niitaka' nursery stock by supplemental planting method. Fruit nursery stock planted without warming treatment. Fruit nursery stock planted with intermittent warming treatment ( ). Fruit nursery stock planted with intermittent warming treatment ( )

92 Fig. 29. Final growth of pear 'Niitaka' nursery stock by supplemental planting method. Fruit nursery stock planted without warming treatment. Fruit nursery stock planted with intermittent warming treatment ( ). Fruit nursery stock planted with intermittent warming treatment ( )

93 Fig. 30. Final shoot number of pear 'Niitaka' nursery stock by supplemental planting method. Fruit nursery stock planted without warming treatment. Fruit nursery stock planted with intermittent warming treatment ( ). Fruit nursery stock planted with intermittent warming treatment ( )

94 6 7, 8 (Fig. 31, 32, 33). ) ( ) , (Fig. 34, 35, 36). ) ( ), , (Fig. 37, 38, 39). ) ( ) (Fig. 40, 41, 42)

95 Fig. 31. Survival rate of grape 'Sheridan' nursery stock by supplemental planting method. Fruit nursery stock planted without warming treatment. Fruit nursery stock planted with intermittent warming treatment ( ). Fruit nursery stock planted with intermittent warming treatment ( )

96 Fig. 32. Final growth of grape 'Sheridan' nursery stock by supplemental planting method. Fruit nursery stock planted without warming treatment. Fruit nursery stock planted with intermittent warming treatment ( ). Fruit nursery stock planted with intermittent warming treatment ( )

97 Fig. 33. Final shoot number of grape 'Sheridan' nursery stock by supplemental planting method. Fruit nursery stock planted without warming treatment. Fruit nursery stock planted with intermittent warming treatment ( ). Fruit nursery stock planted with intermittent warming treatment ( )

98 Fig. 34. Survival rate of peach 'Yumyoung' nursery stock by supplemental planting method. Fruit nursery stock planted without warming treatment. Fruit nursery stock planted with intermittent warming treatment ( ). Fruit nursery stock planted with intermittent warming treatment ( )

99 Fig. 35. Final growth of peach 'Yumyoung' nursery stock by supplemental planting method. Fruit nursery stock planted without warming treatment. Fruit nursery stock planted with intermittent warming treatment ( ). Fruit nursery stock planted with intermittent warming treatment ( )

100 Fig. 36. Final shoot number of peach 'Yumyoung' nursery stock by supplemental planting method. Fruit nursery stock planted without warming treatment. Fruit nursery stock planted with intermittent warming treatment ( ). Fruit nursery stock planted with intermittent warming treatment ( )

101 Fig. 37. Survival rate of persimmon 'Fuyu' nursery stock by supplemental planting method. Fruit nursery stock planted without warming treatment. Fruit nursery stock planted with intermittent warming treatment ( ). Fruit nursery stock planted with intermittent warming treatment ( )

102 Fig. 38. Final growth of persimmon 'Fuyu' nursery stock by supplemental planting method. Fruit nursery stock planted without warming treatment. Fruit nursery stock planted with intermittent warming treatment ( ). Fruit nursery stock planted with intermittent warming treatment ( )

103 Fig. 39. Final shoot number of persimmon 'Fuyu' nursery stock by supplemental planting method. Fruit nursery stock planted without warming treatment. Fruit nursery stock planted with intermittent warming treatment ( ). Fruit nursery stock planted with intermittent warming treatment ( )

104 Fig. 40. Survival rate of kiwifruit 'Hayward' nursery stock by supplemental planting method. Fruit nursery stock planted without warming treatment. Fruit nursery stock planted with intermittent warming treatment ( ). Fruit nursery stock planted with intermittent warming treatment ( )

105 Fig. 41. Final growth of kiwifruit 'Hayward' nursery stock by supplemental planting method. Fruit nursery stock planted without warming treatment. Fruit nursery stock planted with intermittent warming treatment ( ). Fruit nursery stock planted with intermittent warming treatment ( )

106 Fig. 42. Final shoot number of kiwifruit 'Hayward' nursery stock by supplemental planting method. Fruit nursery stock planted without warming treatment. Fruit nursery stock planted with intermittent warming treatment ( ). Fruit nursery stock planted with intermittent warming treatment ( )

107 2. N, P, K, Ca, Mg, 0 (Table 14, 15, 16, 17, 18, 19)

108 Table 14. Mineral and carbohydrate contents in stem of apple 'Fuji' nursery stock by supplemental planting method. Month Mineral (%) N P K Ca Mg Carbohydrate (%) z y z y z y z y z) Fruit nursery stock planted with intermittent warming treatment( ). y) Fruit nursery stock planted with intermittent warming treatment( )

109 Table 15. Mineral and carbohydrate contents in stem of pear 'Niitaka' nursery stock by supplemental planting method. Month Mineral (%) N P K Ca Mg Carbohydrate (%) az 0.173a 0.720a 0.735a 0.078a 9.5a z y z y z y z y z) Fruit nursery stock planted with intermittent warming treatment( ). y) Fruit nursery stock planted with intermittent warming treatment( )

110 Table 16. Mineral and carbohydrate contents in stem of grape 'Sheridan' nursery stock by supplemental planting method. Month Mineral (%) N P K Ca Mg Carbohydrate (%) az 0.203a 0.620a 0.543a 0.100a 7.9a z y z y z y z y z) Fruit nursery stock planted with intermittent warming treatment( ). y) Fruit nursery stock planted with intermittent warming treatment( )

111 Table 17. Mineral and carbohydrate contents in stem of peach 'Yumyoung' nursery stock by supplemental planting method. Month Mineral (%) N P K Ca Mg Carbohydrate az 0.183a 0.590a 1.023a 0.137a 11.5a z y z y z y z y z) Fruit nursery stock planted with intermittent warming treatment( ). y) Fruit nursery stock planted with intermittent warming treatment( )

112 Table 18. Mineral and carbohydrate contents in stem of persimmon 'Fuyu' nursery stock by supplemental planting method. Month Mineral (%) N P K Ca Mg Carbohydrate (%) az 0.099a 0.830a 0.613a 0.062a 8.5a z y z y z y z y z) Fruit nursery stock planted with intermittent warming treatment( ). y) Fruit nursery stock planted with intermittent warming treatment( )

113 Table 19. Mineral and carbohydrate contents in stem of kiwifruit 'Hayward' nursery stock by supplemental planting method. Month Mineral (%) N P K Ca Mg Carbohydrate (% ) az 0.133a 0.440a 0.393a 0.082a 6.9a z y z y z y z y z) Fruit nursery stock planted with intermittent warming treatment( ). y) Fruit nursery stock planted with intermittent warming treatment( )

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119 6. Brown, C. S., E. Young and D. M. Pharr Rootstock and scion effects on the seasonal distribution of dry weight and carbohydrates in young apple trees. J. Am. Soc. Hort. Sci. 110: Buwalda, J. G. and G. S. Smith Accumulation and partitioning of dry matter and mineral nutrients in developing kiwifruits vines. Tree Physiol. 3; Davis, T.S., and T. R. Potter Carbohydrates, water potential and subsequent rooting of stored rhododendron cuttings. HortScience:20: Dowler, W. M. and F. King Seasonal changes in starch and soluble sugar content of dormant peach tissues. Proc. Am. Soc. Hort. Sci. 89: Flint, H. L., and J. J. McGuire Response of rooted cuttings of several woody ornamental species to overwinter storage. Proc. Amer. Soc. Hort. Sci. 80: Hocking, D., and R. D. Nyland Cold storage of coniferous seedlings. AFRI Res. Rpt. 6, Syracuse Univ. Col. Forestry, Syracuse, N. Y. Kandiah, S a. Turnover of carbohydrates in relation to growth in apple trees. I. Seasonal variation of growth and carbohydrates reserves. Ann. Bot. 44:

120 Lutz, J. M., and R. E. Hardenberg The commercial storage of fruits, vegetables, and florist and nursery stocks, USDA-ARS Agr. Handbook 66. Washington, D. C.: U. S. Govt. Printing Office. Mahlstede, J. P., and W. E. Fletcher Storage of nursery stock. Washington, D. C.: Ameri. Assn. Nurs., pp.1-62 Murneek, A. E Quantitative distribution of nitrogen and carbohydrates in apple trees. Res. Bull. Mo. Agr. Expt. Sta Murneek, A.E Carbohydrate storage in apple trees. Proc. Am. Soc. Hort. Sci. 30: Pryor, R. L.,and R. N Stewart Storage of unrooted azalea cuttings. Proc. Amer. Soc. Hort. sci. 82: Smith, G. S., J. clark, and H. V. Henderson c. Seasonal accumulation of mineral nutrients by kiwifruit. 1. Leaves. New Phytol 106: Synder, W. E., and C. E. Hess Low Temperature storage of rooted cuttings of nursery crops. Proc. Amer. Soc. Hort. Sci. 67:

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