한수지 53(5), , 2020 Original Article Korean J Fish Aquat Sci 53(5), , 년서해연안패류및양식새우 (Litopenaeus vannamei) 에서분리한 Vibrio parahaemoly

Size: px
Start display at page:

Download "한수지 53(5), , 2020 Original Article Korean J Fish Aquat Sci 53(5), , 년서해연안패류및양식새우 (Litopenaeus vannamei) 에서분리한 Vibrio parahaemoly"

Transcription

1 한수지 53(5), , 2020 Original Article Korean J Fish Aquat Sci 53(5), , 년서해연안패류및양식새우 (Litopenaeus vannamei) 에서분리한 Vibrio parahaemolyticus 균주들과새우급성간췌장괴사병 (AHPND) 과의연관성 장광일 박진일 오은경 * 김수미 1 * 국립수산과학원서해수산연구소, 1 국립수산과학원수산방역과 The Relationship Between Acute Hepatopancreatic Necrosis Disease (AHPND) in Shrimp Litopenaeus vannamei and Vibrio parahaemolyticus Strains Isolated from Shellfish and Shrimp of the West Coast of Korea in 2019 Gwang Il Jang, Jin Il Park, Eun-Gyoung Oh* and Sumi Kim 1 * West Sea Fisheries Research Institute, National Institute of Fisheries Science, Incheon 22383, Korea 1 Aquatic Disease Control Division, National Institute of Fisheries Science, Busan 46083, Korea Acute hepatopancreatic necrosis disease (AHPND), previously known as early mortality syndrome (EMS), is an emerging disease in shrimp caused by Vibrio parahaemolyticus. Some V. parahaemolyticus strains are associated with foodborne diseases in humans. To date, studies on the relationship between AHPND and pathogenic V. parahaemolyticus are very limited. In this study, we monitored the thermostable direct hemolysin-related hemolysin (trh) gene and AHPND related genes, such as Photorhabdus insect-related (pir) genes, in 892 strains of V. parahaemolyticus isolated and identified in 24 areas of the West Coast of Korea from May to October The trh gene was detected in 9.6% of the isolates from short neck clam samples. However, the pira and pirb genes related to AHPND were not found in any of the isolates despite using both duplex and nested PCR assays, suggesting that AHPND-related genes were nonexistent in the V. parahaemolyticus strains isolated. This study contributes to the current understanding of the relationship between AHPND and V. parahaemolyticus in Korea, as well as provides data on spatial and seasonal distributions of V. parahaemolyticus. Keywords: Acute hepatopancreatic necrosis disease (AHPND), Vibrio parahaemolyticus, Shrimp, West Coast, Thermostable direct hemolysin-related hemolysin (trh) gene 서론 Vibrio (genus),, 140 (Parte, 2018; Urakawa et al., 2000). (Kongrueng et al., 2015) 12 Vibrio, Vibrio parahaemolyticus, Vibrio vulnificus, Vibrio cholera, Vibrio mimicus, Vibrio hollisae, Vibrio alginolyticus, Vibrio cincinnatiensis, Vibrio damsela, Vibrio fluvialis, Vibrio furnissii, Vibrio harveyi, Vibrio metschnikovii (Farmer et al., 2015; CDC, 2019). (V. cholera), (V. parahaemolyticus), (V. vulnificus) (Thompson et al., 2006)., (V. para- *Corresponding author: Tel: Fax: address: This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Licens ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Korean J Fish Aquat Sci 53(5), , October 2020 Received 9 July 2020; Revised 19 August 2020; Accepted 10 September 2020 저자직위 : 장광일 ( 연구사 ), 박진일 ( 연구사 ), 오은경 ( 연구관 ), 김수미 ( 주무관 ) Copyright 2020 The Korean Society of Fisheries and Aquatic Science 752 pissn: , eissn:

2 Vibrio parahaemolyticus 균주와새우급성간췌장괴사병 (AHPND) 과의연관성 753 haemolyticus) (Kang et al., 2016; Yang et al., 2017; Park et al., 2018) (thermostable direct hemolysin, TDH) (TDH-related hemolysin, TRH)., V. parahaemolyticus,, (Sudheeshand and Xu, 2001; Marudhupandi et al., 2017). (acute hepatopancreatic necrosis disease, AHPND) V. parahaemolyticus (Lightner et al., 2012; Zhang et al., 2012) AHPND. V. parahaemolyticus AHPND, AHPND. (Photorhabdus insect-related, Pir) plasmid AHPND (Joshi et al., 2014; Han et al., 2015; Kim and Kim, 2015). V. parahaemolyticus. AHPND 2009 (2010 ), (2011 ), (2012 ), (2013 ), 2017 (Zorriehzahra and Banaederakhshan, 2015; Dhar et al., 2019) Tran et al. (2013) AHPND V. parahaemolyticus, V. parahaemolyticus AHPND V. parahaemolyticus type IV pilus/type IV plasmid (pir toxin) (Kondo et al., 2014; Yang et al., 2014)., Han et al. (2015) AHPND V. parahaemolyticus plasmid (pira/pirb) AHPND (Hwang et al., 2018). V. parahaemolyticus AHPND (Tran et al., 2013; Kondo et al., 2014; Han et al., 2015; Lee et al., 2015), AHPND V. parahaemolyticus V. parahaemolyticus (thermostable direct hemolysin, TDH) (TDH-related hemolysin, TRH) (tdh, trh) (V. parahaemolyticus) (AHPND). 재료및방법 시료채취 V. parahaemolyticus 18, (Muuido), (Yeongheungdo), (Soijakdo), (Seungbongdo), (Cheonsu Bay), (Wonsando), (Iwon-myeon), (Garorim Bay), (Sowon-myeon), (Byeonsan), (Gochang-gun), (Dolipo), (Aphaedo), (Jangsando), (Hampyeong Bay), (Tando Bay), (Maehwado), (Chilsando) 4, (Ganghwa-gun), (Pyeongtaek-si), 13, , Incheon 3 Gyeonggi-do Chungcheongnam-do Jeollabuk-do Jeollanam-do Fig. 1. Sampling sites of shellfish and shrimp Litopenaeus vannamei on the West Coast of Korea during the period May to October in The description of sampling sites is the same as in Table 2.

3 754 장광일ㆍ박진일ㆍ오은경ㆍ김수미 (Taean-gun) (Boryeong-si), (Oyster Crassostrea gigas), (Surf clam Mactra veneriformis), (Short neck clam Venerupis philippinarum), (Ocean quahog Arctica islandica), (Conch Lobatus gigas), (Abalone Haliotis discus hannai) (Shrimp Litopenaeus vannamei) (pond water) (Fig. 1; Table 1)., 10 C. V. parahaemolyticus 균주분리및동정 V. parahaemolyticus (food and drug administration, FDA) bacteriological analytical manual (BAM) (Elliot et al., 1995). ( ) phosphate buffered saline (PBS: 140 mm NaCl, 5 mm anhydrous Na 2 HPO 4, 1.5 mm KH 2 PO 4, ph 7.4), alkaline peptone water (APW; peptone 10 g/l, NaCl 20 g/l, ph ) C (Feng et al., 2017; Yang et al., 2019)., CHROMagar (Paris, France) 35 C 24. CHROMagar V. parahaemolyticus (colony). V. parahaemolyticus, 42 C, ONPG (O-nitrophenyl- -D-galactopyranoside), VITEK system (BioMérieux Vitek, Marcy-l'Étoile, France) V. parahaemolyticus (Park et al., 2019; Song et al., 2020). DNA 추출및병원성관련유전자탐색 tdh trh PCR (polymerase chain reaction). tdh trh VPD- 1/VPD-2 VPR-1/VPR-2 primer sets (Takara, Bio Inc., Otsu, Japan). Tryptic Soy Broth (Difco, Detroit, MU, USA) 10 ml 37 C 18-24, DNA Isolation kit (MO- BIO, Carlsbad, CA, USA) DNA. tdh trh PCR Master mix (Takara Bio Inc., Otsu, Japan) 12.5 μl, primer (10 pmol/μl) 0.2 μl, DNA 2 μl, 25 μl. thermal cycle (Takara Bio Inc., Otsu, Japan) 98 C 10, 55 C 30, 72 C PCR. PCR gel documentation system G BOX (Syngene, Cambridge, UK) amplicon (tdh, 251 bp; trh, 250 bp). (AHPND) (world organization for animal health, OIE) (duplex PCR AP4 nested PCR) (Dangtip et al., 2015; Han et al., 2015). V. parahaemolyticus plasmid Pir toxin AccuPower HotStart PCR premix kit (Bioneer, Korea) VpPirA-284F/R VpPirB-392F/R primer sets duplex PCR (Table 1; Han et al., 2015). Dangtip et al. (2015) AP4-F1/AP4-R1 primers PCR, PCR AP4-F2/AP4-R2 primers AP4 nested PCR (Table 1). PCR QIAxcel Advanced (Qiagen, Hilden, Germany). 결과및고찰 패류및새우양식장의 V. parahaemolyticus 분포 V. parahaemolyticus,, V. parahaemolyticus Table 2. Table 1. PCR primers and sequences of Photorhabdus insect-related (pir) genes used in this study Genes Primers Product (bp) References Photorhabdus insect-related (pir) gene VpPirA-284F: 5'-TGA-CTA-TTC-TCA-CGA-TTG-GAC-TG-3' VpPirA-284R: 5'-CAC-GAC-TAG-CGC-CAT-TGT-TA-3' VpPirB-392F: 5'-TGA-TGA-AGT-GAT-GGG-TGC-TC-3' VpPirB-392R: 5'-TGT-AAG-CGC-CGT-TTA-ACT-CA-3' AP4-F1: 5'-ATG-AGT-AAC-AAT-ATA-AAA-CAT-GAA-AC-3' AP4-R1: 5'-ACG-ATT-TCG-ACG-TTC-CCC-AA-3' AP4-F2: 5'-TTG-AGA-ATA-CGG-GAC-GTG-GG-3' AP4-F2: 5'- GTT-AGT-CAT-GTG-AGC-ACC-TTC-3' Han et al., 2015 Dangtip et al., 2015

4 Vibrio parahaemolyticus 균주와새우급성간췌장괴사병 (AHPND) 과의연관성 755 V. parahaemolyticus (MPN/100 g) 1.E+05 1.E+04 1.E+03 1.E+02 1.E+01 1.E+00 Site 13 Site 14 Site 19 Site 20 May June July August September October November Fig. 2. Monthly variation of V. parahaemolyticus isolated from Oyster Crassostrea gigas farm on the West Coast of Korea. The description of sampling sites is the same as in Table , 20, 204, 4, 125, 147, , , , , , (Table 2). 5 10, V. parahaemolyticus 8 10 MPN (most probable number) (Fig. 2). Park et al. (2019) V. parahaemolyticus %. Lee et al. (2019) 3 (,, ) V. parahaemolyticus, 8 [ colony form unit (CFU)/g], 10 ( CFU/g), 14.6 C V. parahaemolyticus (Lee et al., 2008; Yu et al., 2014; Kim et al., 2016), 5 10 V. parahaemolyticus V. parahaemolyticus 23.1% 26.7% (Table2). (26- M NC PC Fig. 3. Representative results of PCR of the AHPND related genes (pira/pirb) using the duplex PCR for V. parahaemolyticus isolated from shellfish and shrimp Litopenaeus vannamei on the West Coast of Korea. M, size marker; 1, Shrimp (1906 DS-W1); 2, Pond water (1909 DS-W13); 3, Oyster (JN 16-11); 4, Short neck clam (IC 2-1); 5, Conch (CN 4-13); 6, Abalone (CN 3-19); 7, Surf clam (JB 1-7); 8, Ocean quahog (JN 19-20); NC, negative control; PC, positive control. 28 C) (10-15 psu) V. parahaemolyticus, (20-30 C) (4-80 psu) V. parahaemolyticus (Farmer et al., 2015; NIFS, 2018). 병원성관련유전자검출 내열성용혈독소 (tdh) 및내열성용혈독소유사독소 (trh) 유전자검출 V. parahaemolyticus 892

5 756 장광일ㆍ박진일ㆍ오은경ㆍ김수미 (tdh) (trh) tdh, trh 10 5 (9.6%) (Table 3) V. parahaemolyticus tdh trh, tdh %, trh % tdh, V. parahaemolyticus 37 tdh trh (Park et al., 2016). Kang et al. (2017) toxr V. parahaemolyticus 31 tdh trh 4 (9.1%) trh, tdh. V. parahaemolyticus. V. parahaemolyticus (Thompson et al., 1976; Honda and Iida,., 1993; Yamamoto et al., 2008; Malcolm et al., 2015). Table 2. Number of isolates from shellfish and shrimp Litopenaeus vannamei on the West Coast of Korea during the period May to October in 2019 Sites Sampling sites Samples May June July August September October 1 Ganghwa-gun, Incheon #1 - - (2*) 4 (4*) 9 (1*) 5 (21*) 8 2 Ganghwa-gun, Incheon #2-8 (6*) 10 (6*) 9 (4*) 19 (17*) 8 (5*) 3 Pyeongtaek-si, Gyeonggi-do Shrimp Litopenaeus vannamei (Pond water*) (10*) 3 (20*) 12 (19*) 6 (14*) 4 Taean-gun, Chungcheongnam-do (3*) - (7*) Boryeong-si, Chungcheongnam-do - 4 (8*) Jung-gu, Incheon (Muuido) 7 Seocheon-gun, Chungcheongnam-do (Cheonsu Bay) Boryeong-si, Chungcheongnam-do (Wonsando) Short neck clam Buan-gun, Jeollabuk-do (Byeonsan) Venerupis philippinarum Muan-gun, Jeollanam-do (Dolipo) Sinan-gun, Jeollanam-do (Aphaedo) Sinan-gun, Jeollanam-do (Jangsando) Taean-gun, Chungcheongnam-do (Iwon-myeon#1) Taean-gun, Chungcheongnam-do (Iwon-myeon #4) Hampyeong-gun, Jeollanam-do (Hampyeong Bay) Oyster Crassostrea gigas Muan-gun, Jeollanam-do (Tando Bay) Sinan-gun, Jeollanam-do (Maehwado) Ongjin-gun, Incheon (Yeongheungdo) Short neck clam (4 ) 19 Ongjin-gun, Incheon (Soijakdo) Venerupis philippinarum - 8 (1 ) - (21 ) 14 (17 ) 20 (36 ) 31 (19 ) 20 Ongjin-gun, Incheon (Seungbongdo) (Oyster Crassostrea gigas ) (5 ) 12 (29 ) 12 (9 ) 7 (11 ) Taean-gun, Chungcheongnam-do (Garorim Bay) Taean-gun, Chungcheongnam-do (Sowon-myeon) Conch Lobatus gigas Abalone Haliotis discus hannai Gochang-gun, Jeollabuk-do Surf clam Mactra veneriformis Yeonggwang-gun, Jeollanam-do 24 Ocean quahog Arctica islandica (Chilsando) * and in parentheses were isolated from pond water and Oyster, respectively. -, no data.

6 Vibrio parahaemolyticus 균주와새우급성간췌장괴사병 (AHPND) 과의연관성 757 (a) M NC PC (b) M NC PC Fig. 4. Representative results of PCR of the AHPND related genes (pira/pirb) using the (a) first PCR and (b) nested PCR for V. parahaemolyticus isolated from shellfish and shrimp on the West Coast of Korea. M, size marker; 1, Shrimp (1906 DS-W1); 2, Pond water (1909 DS-W13); 3, Oyster (JN 16-11); 4, Short neck clam (IC 2-1); 5, Conch (CN 4-13); 6, Abalone (CN 3-19); 7, Surf clam (JB 1-7); 8, Ocean quahog (JN 19-20); NC, negative control; PC, positive control. AHPND 관련유전자검출 V. parahaemolyticus 892 duplex PCR nested PCR AHPND pira pirb (Fig. 3 and Fig. 4, Table 3) AHPND, AHPND AHPND V. parahaemolyticus, 2013 V. parahaemolyticus AHPND (Zhang et al., 2012; Tran et al., 2013). Han et al. (2015) Vibrio Photobacterium 77 AHPND pira/pirb AHPND V. parahaemolyticus pira/pirb., AHPND V. parahaemolyticus pira/pirb. AHPND V. parahaemolyticus pira/pirb. Han et al. (2015) AHPND V. parahaemolyticus 2 1 AHPND. AHPND V. parahaemolyticus 69 kb plasmid (Pir toxins). V. parahaemolyticus AHPND / biofilm (transposition), (conjugation) plasmid pira/pirb Table 3. Detection rates (%) of thermostable direct hemolysin-related hemolysin (trh) gene and AHPND related genes (pira/pirb) in V. parahaemolyticus isolated from shellfish and shrimp Litopenaeus vannamei on the West Coast of Korea during the period May to October in 2019 Areas Number of isolates trh gene Detection rates (%) Detection rates(%) Source of pira/pirb genes Incheon 428 0% - 0% Gyeonggi-do 94 0% - 0% Chungcheongnam-do 284 0% - 0% Jeollabuk-do 34 0% - 0% Jeollanam-do % Short neck clam (October) 0%

7 758 장광일ㆍ박진일ㆍ오은경ㆍ김수미 V. parahaemolyticus. AHPND AHPND V. parahaemolyticus V. parahaemolyticus (Kondo et al., 2014; Yang et al., 2014; Lee et al., 2015; Fu et al., 2017). Fu et al. (2017) 3 V. parahaemolyticus 9 Genbank V. parahaemolyticus 93 V. parahaemolyticus. Fu et al. (2017) V. parahaemolyticus 9 2 pira/pirb AHPND, Genbank V. parahaemolyticus 16 pira/pirb. V. parahaemolyticus FDA_R31 (single nucleotide polymorphisms, SNPs) 35,001 35,889 SNPs. V. parahaemolyticus. V. parahaemolyticus 102. V. parahaemolyticus AHPND. AHPND V. parahaemolyticus V. parahaemolyticus AHPND, V. parahaemolytic AHPND PirA/ PirB. V. parahaemolyticus V. parahaemolyticus AHPND V. parahaemolyticus. AHPND.,. AHPND. 사사 (R ) (R ). References CDC (Centers for Disease Control and Prevention) Vibrio species causing vibriosis. Retrieved from cdc.gov/vibrio/faq.html on May 1, Dangtip S, Sirikharin R, Sanguanrut P, Thitamadee S, Sritunyalucksana K, Taengchaiyaphum S, Mavichak R, Proespraiwong P and Flegel TW AP4 method for two-tube nested PCR detection of AHPND isolates of Vibrio parahaemolyticus. Aquaculture Rep 2, org/ /j.aqrep Dhar AK, Piamsomboon P, Caro LFA, Kanrar S, Adami R Jr and Juan YS First report of acute hepatopancreatic necrosis disease (AHPND) occurring in the USA. Dis Aquat Org 132, Elliot EL, Kaysner CA, Jackson L and Tamplin ML Vibrio cholera, V. parahaemolyticus, V. vulnificus and other Vibrio spp. In: Bacteriological analytical manual. Association of Offical Analytical Chemists ed. FDA, Arlington, U.S.A., Farmer JJ III, Brenner FW, Cameron DN, Birkhead KM and Janda JM Genus Vibrio Pacini 1854, 411 AL In: Bergey s manual of systematics of archaea and bacteria. John Wiley Sons, Hoboken, NJ, U.S.A. org/ / gbm Feng B, Liu H, Wang M, Sun X, Pan Y and Zhao Y Diversity analysis of acute hepatopancreatic necrosis diseasepositive Vibrio parahaemolyticus strains. Aquacul Fish 2, Fu S, Tian H, Wei D, Zhang X and Liu Y Delineating the origins of Vibrio parahaemolyticus isolated from outbreaks of acute hepatopancreatic necrosis disease in asia by the use of whole genome sequencing. Front Microbiol 28, Han JE, Tang KFJ, Tran LH and Lightner DV Photorhabdus insect-related (Pir) toxinlike genes in a plasmid of Vibrio parahaemolyticus, the causative agent of acute hepatopancreatic necrosis disease (AHPND) of shrimp. Dis Aquat Organ 113, Honda T and Iida T The pathogenicity of Vibrio parahaemolyticus and the role of the thermostable direct haemolysin and related haemolysins. Rev Med Microbiol 4, Hwang SD, Hwang JY, Kang BC, Jeon HR, Chun WJ, Lee DW, Sohn SB, Kim SM, Kim SR, Lee KY, Jung MH, Seo JS, Kwon M and Jee BY Whole genome sequence of acute hepatopancreatic necrosis (AHPND) isolated from Korea. In: Proceedings of the Korean Federation of Fisheries Science and Technology Societies (KOFFST) International Conference 2018, Busan, Korea, 492.

8 Vibrio parahaemolyticus 균주와새우급성간췌장괴사병 (AHPND) 과의연관성 759 Joshi J, Srisala J, Truong VH, Chen IT, Nuangsaeng B, Suthienkul O, Lo CF, Flegel TW, Sritunyalucksana K and Thitamadee S Variation in Vibrio parahaemolyticus isolates from a single Thai shrimp farm experiencing an outbreak of acute hepatopancreatic necrosis disease (AHPND). Aquaculture , aquaculture Kang CH, Shin Y, Jang S, Yu H, Kim S, An S, Park K and So JS Characterization of Vibrio parahaemolyticus isolated from oysters in Korea: Resistance to various antibiotics and prevalence of virulence genes. Mar Pollut Bull 118, Kang CH, Shin YJ, Kim WR, Kim YG, Song KC, Oh EG, Kim SK, Yu HS and So JS Prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus isolated from oysters in Korea. Environ Sci Pollut Res 23, doi.org/ /s Kim NE and Kim DH Acute hepatopancreatic necrosis of shrimp and import health measures. J fish Pathol 28, Kim SK, An SR, Park BM, Oh EG, Song KC, Kim JW and Yu HS Virulence factors and antimicrobial susceptibility of Vibrio parahaemolyticus isolated from oyster Crassostrea gigas. Korean J Fish Aquat Sci 49, Kondo H, Tinwongger S, Proespraiwong P, Mavichak R, Unajak S, Nozaki R and Hirono I Draft genome sequences of six strains of Vibrio parahaemolyticus isolated from early mortality syndrome/acute hepatopancreatic necrosis disease shrimp in Thailand. Genome Announc 2, e doi.org/ /genomea Kongrueng J, Yingkajorn M, Bunpa S, Sermwitaya-wong N, Singkhamanan K and Vudhakul V Characterization of Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease in southern Thailand. J Fish Dis 38, Lee CT, Chen IT, Yang YT, Ko TP, Huang YT, Huang JY, Huang MF, Lin SJ, Chen CY, Lin SS, Lightner DV, Wang HC, Wang AH, Wang HC, Hor LI and Lo CF The opportunistic marine pathogen Vibrio parahaemolyticus becomes virulent by acquiring a plasmid that expresses a deadly toxin. Proc Natl Acad Sci U S A 112, org/ /pnas Lee DS, Shin HY, Park KS and Shin IS Distribution of Vibrio parahaemolyticus and Vibrio vulnificus in seawater and shellfish at Gomso Bay. Korean J Fish Aquat Sci 52, Lee JK, Jung DW, Eom SY, Oh SW, Kim Y, Kwak HS and Kim YH Occurrence of Vibrio parahaemolyticus in oysters from Korean retail outlets. Food Control 19, Lightner, DV, Redman, RM, Pantoja, CR, Noble, BL and Tran L Early mortality syndrome affects shrimp in Asia. Retrieved from GAA-Lightner-Jan12.pdf on May 1, Malcolm TTH, Cheah YK, Radzi CWJWM, Kasim FA, Kantilal HK, John TYH, Martinez-Urtazaf J, Nakaguchig Y, Nishibuchig M and Sona R Detection and quantification of pathogenic Vibrio parahaemolyticus in shellfish by using multiplex PCR and loop-mediated isothermal amplification assay. Food Control 47, foodcont Marudhupandi T, Kumar TTA, Prakash S, Balamurugan J, Dhayanithia NB Vibrio parahaemolyticus a causative bacterium for tail rot disease in ornamental fish, Amphiprion sebae. Aquaculture Reports 8, org/ /j.aqrep NIFS (National Institute of Fisheries Science) Standard manual of whiteleg shrimp farming. NIFS, Busan, Korea. Park K, Mok JS, Kwon JY, Ryu AR and Shim KB Seasonal and Spatial Variation of Pathogenic Vibrio Species Isolated from Seawater and Shellfish off the Gyeongnam Coast of Korea in Korean J Fish Aquat Sci 52, Park K, Mok JS, Ryu AR, Kwon JY, Ham IT and Shim KB Occurrence and virulence of Vibrio parahaemolyticus isolated from seawater and bivalve shellfish of the Gyeongnam coast, Korea, in Mar Poll Bull 137, Park YS, Park K, Kwon JY, Yu HS, Lee HJ, Kim JH, Lee TS and Kim PH Antimicrobial reststance and distribution of virulence factors of Vibrio parahaemolyticus isolated from shellfish farms on the southern coast of Korea. Korean J Fish Aquat Sci 49, KFAS Parte AC LPSN-list of prokaryotic names with standing in nomenclature (bacterio.net), 20 years on. Int J Syst Evol Microbiol 68, ijsem Song X, Zang J, Yu W, Shi X and Wu Y Occurrence and Identification of Pathogenic Vibrio Contaminants in Common Seafood Available in a Chinese Traditional Market in Qingdao, Shandong Province. Front Microbiol 11, Sudheesh PS and Xu HS Pathogenicity of Vibrio parahaemolyticus in tiger prawn Penaeus monodon Fabricius: possible role of extracellular proteases. Aquaculture 196, Thompson CA, Vanderzant C and Ray SM Serological and hemolytic characteristics of Vibrio parahaemolyticus from marine sources. J Food Sci 41, Thompson FL, Austin B and Swings J The biology of vibrios. ASM Press copyright, Washington DC, U.S.A.,

9 760 장광일ㆍ박진일ㆍ오은경ㆍ김수미 Tran L, Nunan L, Redman RM, Mohney LL, Pantoja CR, Fitzsimmons K and Lightner DV Determination of the infectious nature of the agent of acute hepatopancreatic necrosis syndrome affecting penaeid shrimp. Dis Aquat Organ 105, Urakawa H, Yoshida T, Nishimura M and Ohwada K Characterization of depth-related population variation in microbial communities of a coastal marine sediment using 16S rdna-based approaches and quinone profiling. Environ Microbiol 2, Yamamoto A, Iwahori J, Vuddhakul V, Charernjiratrag W, Vose D, Osaka K, Shigematsu M, Toyoufuku H, Yamamoto S, Nishibuchi M and Kasuga F Quantitative modelling for risk assessment of Vibrio parahaemolyticus in bloody clams in southern Thailand. Int J Food Microbiol 124, Yang JH, Mok JS, Jung YJ, Lee KJ, Kwon JY, Park K, Moon SY, Kwon SJ, Ryu AR and Lee TS Distribution and antimicrobial susceptibility of Vibrio species associated with zooplankton in coastal area of Korea. Mar Poll Bull 125, Yang Q, Dong X, Xie G, Fu S, Zou P, Sun J, Wang Y and Huang J Comparative genomic analysis unravels the transmission pattern and intra-species divergence of acute hepatopancreatic necrosis disease (AHPND)-causing Vibrio parahaemolyticus strains. Mol Genet Genomics 294, Yang YT, Chen IT, Lee CT, Chen CY, Lin SS, Hor LI, Tseng TC, Huang YT, Sritunyalucksana K, Thitamadee S, Wang HC and Lo CF Draft genome sequences of four strains of Vibrio parahaemolyticus, three of which cause early mortality syndrome/acute hepatopancreatic necrosis disease in shrimp in China and Thailand. Genome Announc 2, e Yu HS, Oh EG, Shin SB, Park YS, Lee HJ, Kim JH and Song KC Distribution and antimicrobial resistance of Vibrio parahaemolyticus isolated from Korean shellfish. Korean J Fish Aquat Sci 47, KFAS Zhang BC, Liu F, Bian HH, Liu J, Pan LQ and Huang J Isolation, identification, and pathogenicity analysis of a Vibrio parahaemolyticus strain from Litopenaeus vannamei. Prog Fish Sci 33, pone Zorriehzahra MJ and Banaederakhshan R Early mortality syndrome (EMS) as new emerging threat in shrimp industry. Adv Anim Vet Sci 3,

Technical/Specs Sequencing 서비스안내 Standard Sequencing Plasmid/PCR product 를 primer 를이용하여고객이원하는 region 을분석하는서비스이며, 대부분의 normal 샘플에대해 최적화되어있습니다. Full Len

Technical/Specs Sequencing 서비스안내 Standard Sequencing Plasmid/PCR product 를 primer 를이용하여고객이원하는 region 을분석하는서비스이며, 대부분의 normal 샘플에대해 최적화되어있습니다. Full Len Technical/Specs Sequencing 서비스안내 Standard Sequencing Plasmid/PCR product 를 primer 를이용하여고객이원하는 region 을분석하는서비스이며, 대부분의 normal 샘플에대해 최적화되어있습니다. Full Length Sequencing (Primer walking) 길이가긴 insert 를포함한 plasmid

More information

한수지 47(5), , 2014 Original Article Kor J Fish Aquat Sci 47(5), ,2014 MPN 및 H-NS 유전자를표적으로하는 PCR assay 를병용한장염비브리오 (Vibrio parahaemolyticus)

한수지 47(5), , 2014 Original Article Kor J Fish Aquat Sci 47(5), ,2014 MPN 및 H-NS 유전자를표적으로하는 PCR assay 를병용한장염비브리오 (Vibrio parahaemolyticus) 한수지 47(5), 556-561, 2014 Original Article Kor J Fish Aquat Sci 47(5),556-561,2014 MPN 및 H-NS 유전자를표적으로하는 PCR assay 를병용한장염비브리오 (Vibrio parahaemolyticus) 의정량 김태옥 박권삼 * 군산대학교식품생명공학과 Quantification of Vibrio

More information

ƯÇãû

ƯÇãû '' - 1 - - 2 - - 3 - - 4 - - 5 - ' - 6 - - 7 - - 8 - - 9 - - 10 - - 11 - Super-Bio Co., Ltd. KWON, Suk-Tae Thermostable DNA Polymerase-encoding Gene from Thermus sp. X-1 an d Amino Acid Sequence

More information

386-390.hwp

386-390.hwp 386 HANYANG MEDICAL REVIEWS Vol. 29 No. 4, 2009 우리나라 미숙아의 통계와 의료비용 Statistics and Medical Cost of Preterm in Korea 윤혜선 을지대학교 노원을지병원 소아청소년과학교실 Hye Sun Yoon, M.D., Ph.D., Department of Pediatrics, Nowon

More information

Lumbar spine

Lumbar spine Lumbar spine CT 32 111 DOI : 10.3831/KPI.2010.13.2.111 Lumbar Spine CT 32 Received : 10. 05. 23 Revised : 10. 06. 04 Accepted : 10. 06. 11 Key Words: Disc herniation, CT scan, Clinical analysis The Clinical

More information

433대지05박창용

433대지05박창용 Recent Changes in Summer Precipitation Characteristics over South Korea Changyong Park* JaYeon Moon** Eun-Jeong Cha*** Won-Tae Yun**** Youngeun Choi***** 1958 2007 6 9 6 9 10 10 10 10 10 Abstract This

More information

THE JOURNAL OF KOREAN INSTITUTE OF ELECTROMAGNETIC ENGINEERING AND SCIENCE Nov.; 26(11),

THE JOURNAL OF KOREAN INSTITUTE OF ELECTROMAGNETIC ENGINEERING AND SCIENCE Nov.; 26(11), THE JOURNAL OF KOREAN INSTITUTE OF ELECTROMAGNETIC ENGINEERING AND SCIENCE. 2015 Nov.; 26(11), 985991. http://dx.doi.org/10.5515/kjkiees.2015.26.11.985 ISSN 1226-3133 (Print)ISSN 2288-226X (Online) Analysis

More information

DBPIA-NURIMEDIA

DBPIA-NURIMEDIA Original Article Journal of Apiculture 31(2) : 121~131 (2016) The Most Rapid Detection Method against Korean Sacbrood Virus using Ultra-Rapid Reverse-Transcription Real-Time PCR (URRTRT-PCR) Sang-Hyun

More information

03이경미(237~248)ok

03이경미(237~248)ok The recent (2001-2010) changes on temperature and precipitation related to normals (1971-2000) in Korea* Kyoungmi Lee** Hee-Jeong Baek*** ChunHo Cho**** Won-Tae Kwon*****. 61 (1971~2000) 10 (2001~2010).

More information

untitled

untitled 3. 농업환경연구과 과제구분 기본연구 수행시기 전반기 연구과제 및 세부과제 수행 기간 소 속 책임자 농가에 적합한 부식성곤충 대량 사육기술 개발 12~ 13 농업환경연구과 곤충팀 이영혜 1) 부식성 곤충 먹이 제조 기술 개발 12~ 13 농업환경연구과 곤충팀 이영혜 색인용어 부식성곤충, 장수풍뎅이, 계통, 먹이제조 ABSTRACT In first check,

More information

139~144 ¿À°ø¾àħ

139~144 ¿À°ø¾àħ 2 139 DOI : 10.3831/KPI.2010.13.2.139 2 Received : 10. 04. 08 Revised : 10. 04. 26 Two Case Report on Wrist Ganglion Treated with Scolopendrid Pharmacopuncture Accepted : 10. 05. 04 Key Words: Wrist Ganglion,

More information

<30312DC1A4BAB8C5EBBDC5C7E0C1A4B9D7C1A4C3A52DC1A4BFB5C3B62E687770>

<30312DC1A4BAB8C5EBBDC5C7E0C1A4B9D7C1A4C3A52DC1A4BFB5C3B62E687770> Journal of the Korea Institute of Information and Communication Engineering 한국정보통신학회논문지(J. Korea Inst. Inf. Commun. Eng.) Vol. 19, No. 2 : 258~264 Feb. 2015 ID3 알고리즘 기반의 귀납적 추론을 활용한 모바일 OS의 성공과 실패에 대한

More information

한수지 47(5), , 2014 Original Article Kor J Fish Aquat Sci 47(5), ,2014 한국연안산패류중 Vibrio parahaemolyticus 의분포및항생제내성특성비교 유홍식 * 오은경 1 신순범 2 박용수

한수지 47(5), , 2014 Original Article Kor J Fish Aquat Sci 47(5), ,2014 한국연안산패류중 Vibrio parahaemolyticus 의분포및항생제내성특성비교 유홍식 * 오은경 1 신순범 2 박용수 한수지 47(5), 5-515, 214 Original Article Kor J Fish Aquat Sci 47(5),5-515,214 한국연안산패류중 Vibrio parahaemolyticus 의분포및항생제내성특성비교 유홍식 * 오은경 1 신순범 2 박용수 이희정 3 김지회 송기철 1 국립수산과학원식품안전과, 1 국립수산과학원서해수산연구소, 2 국립수산과학원남서해수산연구소,

More information

03-서연옥.hwp

03-서연옥.hwp 농업생명과학연구 49(4) pp.31-37 Journal of Agriculture & Life Science 49(4) pp.31-37 Print ISSN 1598-5504 Online ISSN 2383-8272 http://dx.doi.org/10.14397/jals.2015.49.4.31 국가산림자원조사 자료를 적용한 충남지역 사유림경영율 추정 서연옥

More information

22(3)-07.fm

22(3)-07.fm J. Fd Hyg. Safety Vol. 22, No. 3, pp. 179~191 (2007),QWTPCN QH (QQF *[IKGPG CPF 5CHGV[ Available online at http://www.foodhygiene.or.kr Î ÜôÚ ò Ù Â 1 Â 2 Âú äâ Ö 3 Â Ùý* w w w, 1 w tœw, 2 w yw, 3 w yl

More information

한수지 51(5), , 2018 Original Article Korean J Fish Aquat Sci 51(5), ,2018 단순가공어류수산물제조공정중의식품학적위해요소분석 정민철 강민균 장유미 이도하 박슬기 신일식 1 김영목 * 부경대학교식품

한수지 51(5), , 2018 Original Article Korean J Fish Aquat Sci 51(5), ,2018 단순가공어류수산물제조공정중의식품학적위해요소분석 정민철 강민균 장유미 이도하 박슬기 신일식 1 김영목 * 부경대학교식품 한수지 51(5), 518-523, 2018 Original Article Korean J Fish Aquat Sci 51(5),518-523,2018 단순가공어류수산물제조공정중의식품학적위해요소분석 정민철 강민균 장유미 이도하 박슬기 신일식 1 김영목 * 부경대학교식품공학과, 1 강릉원주대학교해양식품공학과 Hazard Analysis of Food Safety

More information

노인정신의학회보14-1호

노인정신의학회보14-1호 제14권 1호 통권 제23호 www.kagp.or.kr 발행인 : 정인과 / 편집인 : 이동우 / 발행처 : 정인과 (152-703) 서울특별시 구로구 구로동 80번지 고려대학교 구로병원 정신과 / TEL : 02-818-6608 / FAX : 02-852-1937 발행일 : 2008년 4월 30일 / 제 작 : (주)엠엘커뮤니케이션 140-846 서울특별시

More information

44-4대지.07이영희532~

44-4대지.07이영희532~ A Spatial Location Analysis of the First Shops of Foodservice Franchise in Seoul Metropolitan City Younghee Lee* 1 1 (R) 0 16 1 15 64 1 Abstract The foodservice franchise is preferred by the founders who

More information

Analyses the Contents of Points per a Game and the Difference among Weight Categories after the Revision of Greco-Roman Style Wrestling Rules Han-bong

Analyses the Contents of Points per a Game and the Difference among Weight Categories after the Revision of Greco-Roman Style Wrestling Rules Han-bong Analyses the Contents of Points per a Game and the Difference among Weight Categories after the Revision of Greco-Roman Style Wrestling Rules Han-bong An 1 & Kyoo-jeong Choi 2 * 1 Korea National Wrestling

More information

04김호걸(39~50)ok

04김호걸(39~50)ok Journal of Environmental Impact Assessment, Vol. 22, No. 1(2013) pp.39~50 Prediction of Landslides Occurrence Probability under Climate Change using MaxEnt Model Kim, Hogul* Lee, Dong-Kun** Mo, Yongwon*

More information

F. Sequencing FSequencing 01. Sequencing Service DNA sequencing Phone: (ext.4 4)

F. Sequencing FSequencing 01. Sequencing Service DNA sequencing Phone: (ext.4 4) FSequencing 01. DNA sequencing Phone: 1588-9788 (ext.4 4) E-mail: sequencing@bioneer.co.kr 01. 242 FAQs 247 개요 상세 설명 바이오니아는 국내 최초로 를 시작하였으며, 매년 개선된 high-throughput sequencing service를 제공하고 있습니다. Plasmid

More information

09권오설_ok.hwp

09권오설_ok.hwp (JBE Vol. 19, No. 5, September 2014) (Regular Paper) 19 5, 2014 9 (JBE Vol. 19, No. 5, September 2014) http://dx.doi.org/10.5909/jbe.2014.19.5.656 ISSN 2287-9137 (Online) ISSN 1226-7953 (Print) a) Reduction

More information

한수지 49(5), , 2016 Original Article Korean J Fish Aquat Sci 49(5), ,2016 곰소만해역해수에서분리한장염비브리오 (Vibrio parahaemolyticus) 의항균제내성및최소발육억제농도의구명 김

한수지 49(5), , 2016 Original Article Korean J Fish Aquat Sci 49(5), ,2016 곰소만해역해수에서분리한장염비브리오 (Vibrio parahaemolyticus) 의항균제내성및최소발육억제농도의구명 김 한수지 49(5), 582-588, 206 Original Article Korean J Fish Aquat Sci 49(5),582-588,206 곰소만해역해수에서분리한장염비브리오 (Vibrio parahaemolyticus) 의항균제내성및최소발육억제농도의구명 김태옥 엄인선 김희대 박권삼 * 군산대학교식품생명공학과, 충북도립대학바이오생명의약과 Antimicrobial

More information

한국성인에서초기황반변성질환과 연관된위험요인연구

한국성인에서초기황반변성질환과 연관된위험요인연구 한국성인에서초기황반변성질환과 연관된위험요인연구 한국성인에서초기황반변성질환과 연관된위험요인연구 - - i - - i - - ii - - iii - - iv - χ - v - - vi - - 1 - - 2 - - 3 - - 4 - 그림 1. 연구대상자선정도표 - 5 - - 6 - - 7 - - 8 - 그림 2. 연구의틀 χ - 9 - - 10 - - 11 -

More information

- 2 -

- 2 - - 2 - - 3 - - 4 - ➀ - 5 - - 6 - α - 7 - - 8 - - 9 - - 10 - - 11 - - 12 - - 13 - - 14 - - 15 - - 16 - - 17 - - 18 - - 19 - - 20 - μ - 21 - ➀ - 22 - - 23 - - 24 - α - 25 - - 26 - - 27 - μ - 28 - μ μ - 29

More information

THE JOURNAL OF KOREAN INSTITUTE OF ELECTROMAGNETIC ENGINEERING AND SCIENCE. vol. 29, no. 10, Oct ,,. 0.5 %.., cm mm FR4 (ε r =4.4)

THE JOURNAL OF KOREAN INSTITUTE OF ELECTROMAGNETIC ENGINEERING AND SCIENCE. vol. 29, no. 10, Oct ,,. 0.5 %.., cm mm FR4 (ε r =4.4) THE JOURNAL OF KOREAN INSTITUTE OF ELECTROMAGNETIC ENGINEERING AND SCIENCE. 2018 Oct.; 29(10), 799 804. http://dx.doi.org/10.5515/kjkiees.2018.29.10.799 ISSN 1226-3133 (Print) ISSN 2288-226X (Online) Method

More information

1. Korea Centers for Disease Control and Prevention. The fifth Korea National Health and Nutrition Examination Survey (KNHANES V-1) 2010. Cheongwon: Korea Centers for Disease Control and Prevention; 2012.

More information

<B8F1C2F72E687770>

<B8F1C2F72E687770> 제1장 제17장 성홍열 및 사슬알균 감염증 사슬알균 (Streptococci)은 증식 중의 특징적으로 쌍 또는 사슬모양의 그람양성 알균 으로 catalase 음성, 통성혐기성균이며, 보통 한천배지에서는 발육이 어렵고 혈액이나 혈청 등을 첨가한 배지에서 잘 발육한다. 포도당이나 다른 탄수화물을 분해하나 포도당 을 분해하여 가스를 생성하지 않는다. 이들은 최소

More information

DBPIA-NURIMEDIA

DBPIA-NURIMEDIA Short ommunication Journal of piculture 33(3) : 221~226 (218) DOI: 1.17519/apiculture.218.9.33.3.221 Detection of Sugar ane (Saccharum officinarum)-specific Gene from Sugar and Sugar-honey younghee Kim,

More information

232 도시행정학보 제25집 제4호 I. 서 론 1. 연구의 배경 및 목적 사회가 다원화될수록 다양성과 복합성의 요소는 증가하게 된다. 도시의 발달은 사회의 다원 화와 밀접하게 관련되어 있기 때문에 현대화된 도시는 경제, 사회, 정치 등이 복합적으로 연 계되어 있어 특

232 도시행정학보 제25집 제4호 I. 서 론 1. 연구의 배경 및 목적 사회가 다원화될수록 다양성과 복합성의 요소는 증가하게 된다. 도시의 발달은 사회의 다원 화와 밀접하게 관련되어 있기 때문에 현대화된 도시는 경제, 사회, 정치 등이 복합적으로 연 계되어 있어 특 한국도시행정학회 도시행정학보 제25집 제4호 2012. 12 : pp.231~251 생활지향형 요소의 근린주거공간 분포특성 연구: 경기도 시 군을 중심으로* Spatial Distribution of Daily Life-Oriented Features in the Neighborhood: Focused on Municipalities of Gyeonggi Province

More information

DBPIA-NURIMEDIA

DBPIA-NURIMEDIA Journal of the Korea Institute of Information and Communication Engineering 한국정보통신학회논문지(J. Korea Inst. Inf. Commun. Eng.) Vol. 19, No. 5 : 1031~1039 May. 2015 정보보호 산업의 경제적 파급효과 및 기여도 분석 김방룡 1 홍재표 2* Economic

More information

<35335FBCDBC7D1C1A42DB8E2B8AEBDBAC5CDC0C720C0FCB1E2C0FB20C6AFBCBA20BAD0BCAE2E687770>

<35335FBCDBC7D1C1A42DB8E2B8AEBDBAC5CDC0C720C0FCB1E2C0FB20C6AFBCBA20BAD0BCAE2E687770> Journal of the Korea Academia-Industrial cooperation Society Vol. 15, No. 2 pp. 1051-1058, 2014 http://dx.doi.org/10.5762/kais.2014.15.2.1051 멤리스터의 전기적 특성 분석을 위한 PSPICE 회로 해석 김부강 1, 박호종 2, 박용수 3, 송한정 1*

More information

한수지 52(2), , 2019 Note Korean J Fish Aquat Sci 52(2), ,2019 국내유통김 (Porphyra sp.) 의미생물오염도평가 노보영 황선혜 1 조용선 1 * 한국식품연구원식품표준센터, 1 한국식품연구원식품분석

한수지 52(2), , 2019 Note Korean J Fish Aquat Sci 52(2), ,2019 국내유통김 (Porphyra sp.) 의미생물오염도평가 노보영 황선혜 1 조용선 1 * 한국식품연구원식품표준센터, 1 한국식품연구원식품분석 한수지 52(2), 80-84, 209 Note Korean J Fish Aquat Sci 52(2),80-84,209 국내유통김 (Porphyra sp.) 의미생물오염도평가 노보영 황선혜 조용선 * 한국식품연구원식품표준센터, 한국식품연구원식품분석센터 Microbial Contamination Levels in Porphyra sp. Distributed in

More information

DBPIA-NURIMEDIA

DBPIA-NURIMEDIA The e-business Studies Volume 17, Number 6, December, 30, 2016:275~289 Received: 2016/12/02, Accepted: 2016/12/22 Revised: 2016/12/20, Published: 2016/12/30 [ABSTRACT] SNS is used in various fields. Although

More information

04±èºÎ¼º

04±èºÎ¼º Introduction of Integrated Coastal Management Program and Sustainable Development of Fishing Villages in Cheonsu Bay Region* Boosung Kim** Abstract : Sustainable Development(SD) is an important concept

More information

인문사회과학기술융합학회

인문사회과학기술융합학회 Vol.5, No.5, October (2015), pp.471-479 http://dx.doi.org/10.14257/ajmahs.2015.10.50 스마트온실을 위한 가상 외부기상측정시스템 개발 한새론 1), 이재수 2), 홍영기 3), 김국환 4), 김성기 5), 김상철 6) Development of Virtual Ambient Weather Measurement

More information

14.531~539(08-037).fm

14.531~539(08-037).fm G Journal of the Korea Concrete Institute Vol. 20, No. 4, pp. 531~539, August, 2008 š x y w m š gj p { sƒ z 1) * 1) w w Evaluation of Flexural Strength for Normal and High Strength Concrete with Hooked

More information

<30352DB1E2C8B9C6AFC1FD2028C8ABB1E2C7F6292036302D36362E687770>

<30352DB1E2C8B9C6AFC1FD2028C8ABB1E2C7F6292036302D36362E687770> 3D 나노-마이크로 프린팅 기술의 현황 홍 기 현 한국기계연구원 부설 재료연구소 표면기술 연구본부 3D Nano-micro Printing Technology Kihyon Hong Korea Institute of Materials Science, Gyeongnam 642-831, Korea Abstract: 최근 3D 프린팅 기술을 이용하여 마이크로, 나노

More information

jaeryomading review.pdf

jaeryomading review.pdf 4 5 6 7 8 9 10 11 12 13 1. S. Kim, H. Y. Jeong, S. K. Kim, S. Y. Choi and K. J. Lee, Nano Lett. 11, 5438 (2011). 2. E. Menard, K. J. Lee, D. Y. Khang, R. G. Nuzzo and J. A. Rogers, Appl. Phys. Lett. 84,

More information

04_이근원_21~27.hwp

04_이근원_21~27.hwp 1) KIGAS Vol. 16, No. 5, pp 21~27, 2012 (Journal of the Korean Institute of Gas) http://dx.doi.org/10.7842/kigas.2012.16.5.21 실험실의 사례 분석에 관한 연구 이근원 이정석 한국산업안전보건공단 산업안전보건연구원 (2012년 9월 5일 투고, 2012년 10월 19일

More information

07.045~051(D04_신상욱).fm

07.045~051(D04_신상욱).fm J. of Advanced Engineering and Technology Vol. 1, No. 1 (2008) pp. 45-51 f m s p» w Á xá zá Ÿ Á w m œw Image Retrieval Based on Gray Scale Histogram Refinement and Horizontal Edge Features Sang-Uk Shin,

More information

한수지 50(4), , 2017 Original Article Korean J Fish Aquat Sci 50(4), ,2017 우리나라주요김생산해역의해수및물김에대한위생학적평가 정상현 신순범 오은경 조미라 1 윤민철 1 이희정 1 손광태 2 *

한수지 50(4), , 2017 Original Article Korean J Fish Aquat Sci 50(4), ,2017 우리나라주요김생산해역의해수및물김에대한위생학적평가 정상현 신순범 오은경 조미라 1 윤민철 1 이희정 1 손광태 2 * 한수지 50(4), 343-35, 207 Original Article Korean J Fish Aquat Sci 50(4),343-35,207 우리나라주요김생산해역의해수및물김에대한위생학적평가 정상현 신순범 오은경 조미라 윤민철 이희정 손광태 2 * 국립수산과학원남해수산연구소양식산업과, 국립수산과학원식품위생가공과, 2 국립수산과학원남동해수산연구소 Sanitary

More information

歯kjmh2004v13n1.PDF

歯kjmh2004v13n1.PDF 13 1 ( 24 ) 2004 6 Korean J Med Hist 13 1 19 Jun 2004 ISSN 1225 505X 1) * * 1 ( ) 2) 3) 4) * 1) ( ) 3 2) 7 1 3) 2 1 13 1 ( 24 ) 2004 6 5) ( ) ( ) 2 1 ( ) 2 3 2 4) ( ) 6 7 5) - 2003 23 144-166 2 2 1) 6)

More information

K O R E A C E N T E R S F O R D I S E A S E C O N T R O L & P R E V E N T I O N PHWR Vol. 5 No. 41 www.cdc.go.kr/phwr 2012 10 12 5 41 ISSN:2005-811X Comparison of drug-susceptibility test to the anti-tuberculosis

More information

10 (10.1) (10.2),,

10 (10.1) (10.2),, Chapter 16 Precipitation Equilibria Copyright 2001 by Harcourt, Inc. All rights reserved. Requests for permission to make copies of any part of the work should be mailed to the following address: Permissions

More information

- 3 - 1 10. 10. 12 1. 12 2. 12. 13 2 14 2.1 14 2.2 17 2.3 18 2.4 19 2.5 21 (1) 21 (2) DNA 23 (3) 24 (4) 16S rrna 25 (5) (Polymerase chain reaction, PCR) 26 (6) PCR Primer 27 2.6 28. / 28-4 - (1) Bioaerosol

More information

03-ÀÌÁ¦Çö

03-ÀÌÁ¦Çö 25 3 (2004 9 ) J Korean Oriental Med 2004;25(3):20-31 1), 2), 3) 1) 2) 3) Grope for a Summary Program about Intellectual Property Protection of Traditional Knowledge (TK)etc. Discussed in WIPO Hwan-Soo

More information

Journal of Educational Innovation Research 2018, Vol. 28, No. 4, pp DOI: * A Research Trend

Journal of Educational Innovation Research 2018, Vol. 28, No. 4, pp DOI:   * A Research Trend Journal of Educational Innovation Research 2018, Vol. 28, No. 4, pp.295-318 DOI: http://dx.doi.org/10.21024/pnuedi.28.4.201812.295 * A Research Trend on the Studies related to Parents of Adults with Disabilities

More information

10(3)-09.fm

10(3)-09.fm w y wz 10«3y 253~258 (2010.12.) Journal of Korean Society of Urban Environment ³ w Á» Á Á y w y œw (2010 11 22, 2010 12 9 k) Study on Determine of Detention Pond in Small Developed Area In-Soo Chang ½

More information

도큐-표지

도큐-표지 Study on the use of Animal Bones in Ancient China Bo Fengxian, Xu Chengwei, Liu Wen, Sun Shuping (Northwest A&F University) 我国古代对动物骨头的再利用 卜风贤 许成委 刘雯 孙署萍 西北农林科技大学 Abstract Based on Chinese historical documents,

More information

제 9 도는 6제어항목의 세팅목표의 보기가 표시된 레이더 챠트(radar chart). 제 10 도는 제 6 도의 함수블럭(1C)에서 사용되는 각종 개성화 함수의 보기를 표시하는 테이블. 제 11a 도 제 11c 도까지는 각종 조건에 따라 제공되는 개성화함수의 변화의

제 9 도는 6제어항목의 세팅목표의 보기가 표시된 레이더 챠트(radar chart). 제 10 도는 제 6 도의 함수블럭(1C)에서 사용되는 각종 개성화 함수의 보기를 표시하는 테이블. 제 11a 도 제 11c 도까지는 각종 조건에 따라 제공되는 개성화함수의 변화의 (19) 대한민국특허청(KR) (12) 특허공보(B1) (51) Int. Cl. 5 B66B 1/18 (45) 공고일자 1993년09월28일 (11) 공고번호 특1993-0009339 (21) 출원번호 특1989-0002580 (65) 공개번호 특1989-0014358 (22) 출원일자 1989년03월02일 (43) 공개일자 1989년10월23일 (30) 우선권주장

More information

- 2 -

- 2 - - 2 - α - 3 - - 4 - α - 5 - - 6 - - 7 - - 8 - - 9 - - 10 - - 11 - 번호 유효성분 차나무오일 코퍼설페이트펜타하이드레이트 폴리옥신디 - 12 - - 13 - μ - 14 - α m α α μ - 15 - μ μ - 16 - m μ - 17 - - 18 - - 19 - μ - 20 - μ μ μ - 21 - -

More information

한수지 51(1), 23-30, 2018 Original Article Korean J Fish Aquat Sci 51(1),23-30,2018 넙치 (Paralichthys olivaceus) 근육채취방법에따른 Kudoa septempunctata 진단효율비교 송준영

한수지 51(1), 23-30, 2018 Original Article Korean J Fish Aquat Sci 51(1),23-30,2018 넙치 (Paralichthys olivaceus) 근육채취방법에따른 Kudoa septempunctata 진단효율비교 송준영 한수지 51(1), 23-30, 2018 Original Article Korean J Fish Aquat Sci 51(1),23-30,2018 넙치 (Paralichthys olivaceus) 근육채취방법에따른 Kudoa septempunctata 진단효율비교 송준영 정승희 1 최혜승 2 * 국립수산과학원남동해수산연구소, 1 국립수산과학원병리연구과, 2 국립수산과학원남해수산연구소

More information

<30345F283439372D353034295F313135375FC0CCB5BFC8F15FB5B5B7CEC5CDB3CEC0C720B0BBB1B8BACE20B0E6B0FCBCB3B0E8B0A120C5CDB3CE20B3BBBACEC1B6B8ED2E687770>

<30345F283439372D353034295F313135375FC0CCB5BFC8F15FB5B5B7CEC5CDB3CEC0C720B0BBB1B8BACE20B0E6B0FCBCB3B0E8B0A120C5CDB3CE20B3BBBACEC1B6B8ED2E687770> J of Korean Tunn Undergr Sp Assoc 15(5)497-504(2013) eissn: 2287-4747 http://dx.doi.org/10.9711/ktaj.2013.15.5.497 pissn: 2233-8292 도로터널의 갱구부 경관설계가 터널 내부조명에 미치는 영향에 관한 연구 이미애 1 ㆍ이동희 2 * 1 아이라이트 대표이사 2

More information

534 강경태ㆍ김민주ㆍ박선영ㆍ최종덕ㆍ허민수ㆍ김진수., Choi et al. (1998), Jeong et al. (2004), Ha et al. (2005), Lee (2006), Baker (2016), Vugia et al. (2013) 2003.,,,,. HACC

534 강경태ㆍ김민주ㆍ박선영ㆍ최종덕ㆍ허민수ㆍ김진수., Choi et al. (1998), Jeong et al. (2004), Ha et al. (2005), Lee (2006), Baker (2016), Vugia et al. (2013) 2003.,,,,. HACC 한수지 49(5), 533-540, 2016 Original Article Korean J Fish Aquat Sci 49(5),533-540,2016 HACCP 구축을위한굴 (Crassostrea gigas) 가공공장의위해평가 강경태 김민주 1 박선영 1 최종덕 1 허민수 2 김진수 1 * 한국식품안전관리인증원, 1 경상대학교해양식품생명의학과 / 해양산업연구소,

More information

°Ç°�°úÁúº´5-44È£ÃÖÁ¾

°Ç°�°úÁúº´5-44È£ÃÖÁ¾ K O R E A C E N T E R S F O R D I S E A S E C O N T R O L & P R E V E N T I O N PHWR Vol. 5 No. 44 www.cdc.go.kr/phwr 2012 11 2 5 44 ISSN:2005-811X Vector surveillance after elimination of lymphatic filariasis

More information

2.대상 및 범위(계속) 하천 하천 등급 하천명 연장 (km) 연장 (km) 시점 금회수립현황 종점 지방 하천 함안천 8.40 9.06 경남 함안군 여항면 내곡리 경남 함안군 함안면 함안천(국가)기점 검단천 7.00 3.34 경남 함안군 칠북면 검단리 칠원천 6.70

2.대상 및 범위(계속) 하천 하천 등급 하천명 연장 (km) 연장 (km) 시점 금회수립현황 종점 지방 하천 함안천 8.40 9.06 경남 함안군 여항면 내곡리 경남 함안군 함안면 함안천(국가)기점 검단천 7.00 3.34 경남 함안군 칠북면 검단리 칠원천 6.70 경상남도 고시 제2010-46호 하천기본계획 고시 하천법 제25조 및 같은 법 시행령 제24조의 규정에 따라 수립한 하천 기본계획을 같은 법 시행규칙 제13조에 따라 고시합니다. 2010. 1. 29 경 상 남 도 지 사 1.수립 또는 변경 목적 하천의 효율적인 이용과 계획적이고 체계적인 하천사업추진 및 원활한 유지관리와 하천의 일관된 개발계획을 위하여 하천법

More information

..........5-45..

..........5-45.. K O R E A C E N T E R S F O R D I S E A S E C O N T R O L & P R E V E N T I O N PHWR Vol. 5 No. 45 www.cdc.go.kr 2012 11 9 5 45 ISSN:2005-811X Monitoring of antimicrobial resistance on non-tertiary hospitals

More information

DBPIA-NURIMEDIA

DBPIA-NURIMEDIA The e-business Studies Volume 17, Number 4, August, 30, 2016:319~332 Received: 2016/07/28, Accepted: 2016/08/28 Revised: 2016/08/27, Published: 2016/08/30 [ABSTRACT] This paper examined what determina

More information

2 : (JEM) QTBT (Yong-Uk Yoon et al.: A Fast Decision Method of Quadtree plus Binary Tree (QTBT) Depth in JEM) (Special Paper) 22 5, (JBE Vol. 2

2 : (JEM) QTBT (Yong-Uk Yoon et al.: A Fast Decision Method of Quadtree plus Binary Tree (QTBT) Depth in JEM) (Special Paper) 22 5, (JBE Vol. 2 (Special Paper) 22 5, 2017 9 (JBE Vol. 22, No. 5, Sepember 2017) https://doi.org/10.5909/jbe.2017.22.5.541 ISSN 2287-9137 (Online) ISSN 1226-7953 (Print) (JEM) a), a), a) A Fast Decision Method of Quadtree

More information

목차 ⅰ ⅲ ⅳ Abstract v Ⅰ Ⅱ Ⅲ i

목차 ⅰ ⅲ ⅳ Abstract v Ⅰ Ⅱ Ⅲ i 11-1480523-000748-01 배경지역 ( 백령도 ) 에서의 대기오염물질특성연구 (Ⅲ) 기후대기연구부대기환경연구과,,,,,,, Ⅲ 2010 목차 ⅰ ⅲ ⅳ Abstract v Ⅰ Ⅱ Ⅲ i 목차 Ⅳ ii 목차 iii 목차 iv 목차 μg m3 μg m3 v 목차 vi Ⅰ. 서론 Ⅰ μm μg m3 1 Ⅰ. 서론 μg m3 μg m3 μg m3 μm 2

More information

09È«¼®¿µ 5~152s

09È«¼®¿µ5~152s Korean Journal of Remote Sensing, Vol.23, No.2, 2007, pp.45~52 Measurement of Backscattering Coefficients of Rice Canopy Using a Ground Polarimetric Scatterometer System Suk-Young Hong*, Jin-Young Hong**,

More information

06_±è¼öö_0323

06_±è¼öö_0323 166 167 1) 2) 3) 4) source code 5) object code PC copy IP Internet Protocol 6) 7) 168 8) 9)10) 11) 12)13) / / 14) 169 PC publisher End User distributor RPG Role-Playing Game 15) FPS First Person Shooter

More information

- i - - ii - - iii - - iv - - v - - vi - - 1 - - 2 - - 3 - 1) 통계청고시제 2010-150 호 (2010.7.6 개정, 2011.1.1 시행 ) - 4 - 요양급여의적용기준및방법에관한세부사항에따른골밀도검사기준 (2007 년 11 월 1 일시행 ) - 5 - - 6 - - 7 - - 8 - - 9 - - 10 -

More information

°Ç°�°úÁúº´6-2È£

°Ç°�°úÁúº´6-2È£ K O R E A C E N T E R S F O R D I S E A S E C O N T R O L & P R E V E N T I O N PHWR Vol. 6 No. 2 www.cdc.go.kr 2013 1 11 6 2 ISSN:2005-811X Flavivirus surveillance in mosquitoes collected from the quarantine

More information

환경중잔류의약물질대사체분석방법확립에 관한연구 (Ⅱ) - 테트라사이클린계항생제 - 환경건강연구부화학물질연구과,,,,,, Ⅱ 2010

환경중잔류의약물질대사체분석방법확립에 관한연구 (Ⅱ) - 테트라사이클린계항생제 - 환경건강연구부화학물질연구과,,,,,, Ⅱ 2010 11-1480523-000702-01 환경중잔류의약물질대사체분석방법확립에 관한연구 (Ⅱ) - 테트라사이클린계항생제 - 환경건강연구부화학물질연구과,,,,,, Ⅱ 2010 목차 ⅰ ⅱ ⅲ Abstract ⅳ Ⅰ Ⅱ i 목차 Ⅲ Ⅳ i 목차 ii 목차 iii Abstract α β α β iv Ⅰ. 서론 Ⅰ 1 Ⅱ. 연구내용및방법 Ⅱ. 2 Ⅱ. 연구내용및방법

More information

한국전지학회 춘계학술대회 Contents 기조강연 LI GU 06 초강연 김동욱 09 안재평 10 정창훈 11 이규태 12 문준영 13 한병찬 14 최원창 15 박철호 16 안동준 17 최남순 18 김일태 19 포스터 강준섭 23 윤영준 24 도수정 25 강준희 26

한국전지학회 춘계학술대회 Contents 기조강연 LI GU 06 초강연 김동욱 09 안재평 10 정창훈 11 이규태 12 문준영 13 한병찬 14 최원창 15 박철호 16 안동준 17 최남순 18 김일태 19 포스터 강준섭 23 윤영준 24 도수정 25 강준희 26 2015 한국전지학회 춘계학술대회 2일차 한국전지학회 춘계 학술대회(신소재 및 시장동향 관련 주제 발표) 시간 제목 비고 세션 1 차세대 이차전지용 in-situ 분석기술 좌장 : 윤성훈 09:00~09:30 Real-time & Quantitative Analysis of Li-air Battery Materials by In-situ DEMS 김동욱(한국화학연구원)

More information

DBPIA-NURIMEDIA

DBPIA-NURIMEDIA The e-business Studies Volume 17, Number 6, December, 30, 2016:237~251 Received: 2016/11/20, Accepted: 2016/12/24 Revised: 2016/12/21, Published: 2016/12/30 [ABSTRACT] Recently, there is an increasing

More information

Æ÷Àå½Ã¼³94š

Æ÷Àå½Ã¼³94š Cho, Mun Jin (E-mail: mjcho@ex.co.kr) ABSTRACT PURPOSES : The performance of tack coat, commonly used for layer interface bonding, is affected by application rate and curing time. In this study, bonding

More information

<30322EC6AFC1FD30342DC1A4B9AEC7F62E687770>

<30322EC6AFC1FD30342DC1A4B9AEC7F62E687770> 대한내과학회지: 제 77 권 제 2 호 2009 특 집(Special Review) - 신종 감염병의 최신 지견 뎅기열 인하대학교 의학전문대학원 내과학교실 감염내과 정 문 현 Dengue fever Moon-Hyun Chung, M.D. Division of Infectious Diseases, Department of Internal Medicine, College

More information

10(3)-12.fm

10(3)-12.fm w y wz 10«3y 273~280 (2010.12.) Journal of Korean Society of Urban Environment p yá xá½k w y œw (2010 9 15, 2010 12 2 k) Analysis of Characteristics of Delivered Nonpoint Source Pollution at Forested Watershed

More information

07_Àü¼ºÅÂ_0922

07_Àü¼ºÅÂ_0922 176 177 1) 178 2) 3) 179 4) 180 5) 6) 7) 8) 9) 10) 181 11) 12) 182 13) 14) 15) 183 16) 184 185 186 17) 18) 19) 20) 21) 187 22) 23) 24) 25) 188 26) 27) 189 28) 29) 30)31) 32) 190 33) 34) 35) 36) 191 37)

More information

박선영무선충전-내지

박선영무선충전-내지 2013 Wireless Charge and NFC Technology Trend and Market Analysis 05 13 19 29 35 45 55 63 67 06 07 08 09 10 11 14 15 16 17 20 21 22 23 24 25 26 27 28 29 30 31 32 33 36 37 38 39 40

More information

???? 1

???? 1 The Korean Journal of Applied Statistics (2014) 27(1), 13 20 DOI: http://dx.doi.org/10.5351/kjas.2014.27.1.013 Maximum Tolerated Dose Estimation by Stopping Rule and SM3 Design in a Phase I Clinical Trial

More information

학술연구용역과제최종결과보고서

학술연구용역과제최종결과보고서 학술연구용역과제최종결과보고서 최종결과보고서요약문 Summary 학술연구용역과제연구결과 1. 담수어 Table 1. Freshwater fishes a collected from Jiseokcheon (Stream), a branch of Youngsangang (River), located in Naju-si, Jeollanam-do, Korea

More information

(Exposure) Exposure (Exposure Assesment) EMF Unknown to mechanism Health Effect (Effect) Unknown to mechanism Behavior pattern (Micro- Environment) Re

(Exposure) Exposure (Exposure Assesment) EMF Unknown to mechanism Health Effect (Effect) Unknown to mechanism Behavior pattern (Micro- Environment) Re EMF Health Effect 2003 10 20 21-29 2-10 - - ( ) area spot measurement - - 1 (Exposure) Exposure (Exposure Assesment) EMF Unknown to mechanism Health Effect (Effect) Unknown to mechanism Behavior pattern

More information

Can032.hwp

Can032.hwp Chromosomal Alterations in Hepatocellular Carcinoma Cell Lines Detected by Comparative Genomic Hybridization Sang Jin Park 1, Mahn Joon Ha, Ph.D. 1, Hugh Chul Kim, M.D. 2 and Hyon Ju Kim, M.D. 1 1 Laboratory

More information

Problem New Case RETRIEVE Learned Case Retrieved Cases New Case RETAIN Tested/ Repaired Case Case-Base REVISE Solved Case REUSE Aamodt, A. and Plaza, E. (1994). Case-based reasoning; Foundational

More information

SKC_AR_±¹¹® 01pdf

SKC_AR_±¹¹® 01pdf SKC Annual Report 2006 The Next Growth Contents 02 04 06 08 10 12 14 22 24 26 28 40 41 42 44 49 96 98 15,000 12,000 13,263 14,090 12,118 1,200 1,000 962 1,170 10,000 8,000 8,167 9,000 6,000 3,000 800 600

More information

<3230313320C7D8BEE7BCF6BBEAC5EBB0E8BFACBAB82E687770>

<3230313320C7D8BEE7BCF6BBEAC5EBB0E8BFACBAB82E687770> 이용자를 위하여 1. 본 연보에서는 우리부에서 실시한 통계조사와 산하관서의 보고 및 관계기관의 통계 자료 중 중요한 사항을 편집 수록하였음. 2. 본 연보는 주로 2012년말 현재의 통계를 수록하였으며 각표에는 시계열 분석을 할 수 있도록 가급적 최근 5 10년간의 통계를 수록하였음. 3. 본 연보에 사용된 도량형기의 단위는 미터법을 사용하였음. 4. 본 연보에는

More information

A 617

A 617 Special Issue Diabetic Retinopathy Won Ki Lee, M.D. Department of Ophthalmology The Catholic University of Korea College of Medicine Kangnam St. Mary s Hospital E mail : wklee@catholic.ac.kr Abstract R

More information

012임수진

012임수진 Received : 2012. 11. 27 Reviewed : 2012. 12. 10 Accepted : 2012. 12. 12 A Clinical Study on Effect of Electro-acupuncture Treatment for Low Back Pain and Radicular Pain in Patients Diagnosed with Lumbar

More information

(

( 317 318 319 320 1 3 5 5 5 5 2 321 : 1.,,,,, 06 2. X-ray beam penetration (density) (contrast) 03 3. patch coating, precipitation, flaking 03 4. centering 03 5. Esophagus, cardia, fundus, body, angle, antrum,

More information

2019_2nd_guide_kr_0619.indd

2019_2nd_guide_kr_0619.indd CONTENTS 2019 Good Design Award 2 2 2 2 01 02 04 05 06 08 GOOD DESIGN AWARD 2019 Schedule Entry First screening period April 3 Open for Entries May 23 Entry Deadline / Due Date of submission for Application

More information

00내지1번2번

00내지1번2번 www.keit.re.kr 2011. 11 Technology Level Evaluation ABSTRACT The Technology Level Evaluation assesses the current level of industrial technological development in Korea and identifies areas that are underdeveloped

More information

135 Jeong Ji-yeon 심향사 극락전 협저 아미타불의 제작기법에 관한 연구 머리말 협저불상( 夾 紵 佛 像 )이라는 것은 불상을 제작하는 기법의 하나로써 삼베( 麻 ), 모시( 苧 ), 갈포( 葛 ) 등의 인피섬유( 靭 皮 纖 維 )와 칠( 漆 )을 주된 재료

135 Jeong Ji-yeon 심향사 극락전 협저 아미타불의 제작기법에 관한 연구 머리말 협저불상( 夾 紵 佛 像 )이라는 것은 불상을 제작하는 기법의 하나로써 삼베( 麻 ), 모시( 苧 ), 갈포( 葛 ) 등의 인피섬유( 靭 皮 纖 維 )와 칠( 漆 )을 주된 재료 MUNHWAJAE Korean Journal of Cultural Heritage Studies Vol. 47. No. 1, March 2014, pp.134~151. Copyright 2014, National Research Institute of Cultural Heritage 심향사 극락전 협저 아미타불의 제작기법에 관한 연구 정지연 a 明 珍 素 也

More information

untitled

untitled 정책연구 2000-34 과학기술과 지역경제 활성화 및 과학문화 확산방안 Activation of Regional Science Economy and Diffusion of Science Culture 연구기관 단국대학교 과 학 기 술 부 제 출 문 과 학 기 술 부 장 관 귀하 본 보고서를 과학기술과 지역경제 활성화 및 과학문화 확산 방안 최종보고서로 제출합니다.

More information

DBPIA-NURIMEDIA

DBPIA-NURIMEDIA 무선 센서 네트워크 환경에서 링크 품질에 기반한 라우팅에 대한 효과적인 싱크홀 공격 탐지 기법 901 무선 센서 네트워크 환경에서 링크 품질에 기반한 라우팅에 대한 효과적인 싱크홀 공격 탐지 기법 (A Effective Sinkhole Attack Detection Mechanism for LQI based Routing in WSN) 최병구 조응준 (Byung

More information

Analysis of teacher s perception and organization on physical education elective courses Chang-Wan Yu* Korea Institute of curriculum and evaluation [Purpose] [Methods] [Results] [Conclusions] Key words:

More information

½Éº´È¿ Ãâ·Â

½Éº´È¿ Ãâ·Â Standard and Technology of Full-Dimension MINO Systems in LTE-Advances Pro Massive MIMO has been studied in academia foreseeing the capacity crunch in the coming years. Presently, industry has also started

More information

Kor. J. Aesthet. Cosmetol., 및 자아존중감과 스트레스와도 밀접한 관계가 있고, 만족 정도 에 따라 전반적인 생활에도 영향을 미치므로 신체는 갈수록 개 인적, 사회적 차원에서 중요해지고 있다(안희진, 2010). 따라서 외모만족도는 개인의 신체는 타

Kor. J. Aesthet. Cosmetol., 및 자아존중감과 스트레스와도 밀접한 관계가 있고, 만족 정도 에 따라 전반적인 생활에도 영향을 미치므로 신체는 갈수록 개 인적, 사회적 차원에서 중요해지고 있다(안희진, 2010). 따라서 외모만족도는 개인의 신체는 타 RESEARCH ARTICLE Kor. J. Aesthet. Cosmetol., 20-40대 여성의 외모만족도가 미용관리태도에 미치는 영향 홍수남 1, 김효숙 2 * 1 건국대학교 뷰티사이언스디자인학과, 2 건국대학교 의상디자인과 Effects of Extrinsic Body Satisfaction on Beauty Management Behavior of

More information

#Ȳ¿ë¼®

#Ȳ¿ë¼® http://www.kbc.go.kr/ A B yk u δ = 2u k 1 = yk u = 0. 659 2nu k = 1 k k 1 n yk k Abstract Web Repertoire and Concentration Rate : Analysing Web Traffic Data Yong - Suk Hwang (Research

More information

02À±¼ø¿Á

02À±¼ø¿Á Geomorphic Development of Marine Terraces at Jeongdongjin-Daejin area on the East Coast, Central Part of Korean Peninsula Soon-Ock Yoon*, Sang-Ill Hwang** and Hak-Kyun Ban*** Abstract : In this paper we

More information

유해중금속안정동위원소의 분석정밀 / 정확도향상연구 (I) 환경기반연구부환경측정분석센터,,,,,,,, 2012

유해중금속안정동위원소의 분석정밀 / 정확도향상연구 (I) 환경기반연구부환경측정분석센터,,,,,,,, 2012 11-1480523-001163-01 유해중금속안정동위원소의 분석정밀 / 정확도향상연구 (I) 환경기반연구부환경측정분석센터,,,,,,,, 2012 목 차 ⅰ ⅲ ⅳ Abstract ⅵ Ⅰ Ⅱ Ⅲ i 목 차 Ⅳ ii 목 차 iii 목 차 iv 목 차 v Abstract vi Abstract σ ε vii Abstract viii Ⅰ. 서론 Ⅰ. 1 Ⅰ. 서론.

More information

THE JOURNAL OF KOREAN INSTITUTE OF ELECTROMAGNETIC ENGINEERING AND SCIENCE Dec.; 27(12),

THE JOURNAL OF KOREAN INSTITUTE OF ELECTROMAGNETIC ENGINEERING AND SCIENCE Dec.; 27(12), THE JOURNAL OF KOREAN INSTITUTE OF ELECTROMAGNETIC ENGINEERING AND SCIENCE. 2016 Dec.; 27(12), 1036 1043. http://dx.doi.org/10.5515/kjkiees.2016.27.12.1036 ISSN 1226-3133 (Print) ISSN 2288-226X (Online)

More information

07.... 01V28.

07.... 01V28. National Election Commission 9 September S M T W T F S 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23/30 24 25 26 27 28 29 11 November S M T W T F S 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16

More information

141(26) 20056 1930 19 () ( ( ) () () () ) 2) 1932 ()()3) 2 1) 1897519( ) ( ) () () () 4) ( ) 5) 6) 15-20 7) 191216 2) ( ) () 42 () 193111 12 1933 4 3)

141(26) 20056 1930 19 () ( ( ) () () () ) 2) 1932 ()()3) 2 1) 1897519( ) ( ) () () () 4) ( ) 5) 6) 15-20 7) 191216 2) ( ) () 42 () 193111 12 1933 4 3) 141(26) 20056 Korean J Med Hist 14131 Jun 2005 ISSN 1225505X ( 1897-1982) * ** 1 ( 1897-1982) ( ) 1) 3 1 ( ) 1930 1930 1930 * () ** 1) ( 2000 p 13-15) 1 141(26) 20056 1930 19 () ( ( ) () () () ) 2) 1932

More information

Æ÷Àå82š

Æ÷Àå82š Lee, Kyungbae (E-mail : kblee6078@ex.co.kr) Lee, Jaehoon (E-mail : ranian74@ex.co.kr) Sohn, Duecksu (E-mail : mgsds@ex.co.kr) Kwon, Soonmin (E-mail : soonmini2@ex.co.kr) ABSTRACT PURPOSES : The purpose

More information

- iii - - i - - ii - - iii - 국문요약 종합병원남자간호사가지각하는조직공정성 사회정체성과 조직시민행동과의관계 - iv - - v - - 1 - - 2 - - 3 - - 4 - - 5 - - 6 - - 7 - - 8 - - 9 - - 10 - - 11 - - 12 - - 13 - - 14 - α α α α - 15 - α α α α α α

More information

1. (Gastropoda), (Prosobranchia), (Mesogastropoda), (Pleuroceridae),,,. 3 7,.,,.,,.,.. (Habe and Itagaki, 1978), (Nakano and Nishiwaki, 1989), (Koike et al., 1992), (Nakano and Izawa, 1996). (S. forticosta)

More information