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1 The effect of MBCP TM bone regeneration in osteoporosis induced rats Won Hee Kim, Jeong Kyung Kang, Jae Jun Ryu Dept.of esthetic restorative dentistry, Graduate School of Clinical Dentistry, Korea University Abstract Purpose : The purpose of this study is confirming that regeneration capacity of synthetic bone grafted to a skull of osteoporosis-rats caused by ovariectomy differs from that of normal rats. Additionally, this study shows how a general disease like osteoporosis affects to osteointegration or bone regeneration while implanting or bone grafting. Material and Method : 20 rats were selected for the experiment. 10 rats out of 20 rats were performed ovariectomy and induced osteoporosis. Seven weeks later, every 20 rats were formed the defect of critical size at calvary and were performed bone grafting of MBCP TM synthetic bone. Four weeks later, samples were collected from all 20 rats, observed through an light microscope, and measured the size of newly formed bone in the defected area by Image Pro Plus 5.1(Media Cybernetics, LP, Silver Spring, MD, USA) program. After that, measured data between two groups were statistically compared. SPSS Ver. 12.0(SPSS, Chicago, IL, USA) program was used. The concentration of alkaline phosphatase were measured for confirming the induction of osteoporosis. Result : Osteoporosis-rats showed higher concentration of alkaline phoshpatase and more regenerated bone size than normal-rats to a statistically meaningful level. Conclusion : Osteoporosis-rats lacked of bone regeneration compared to normal-rats. Key words :Bone regeneration, MBCP TM, Osteoporosis, Ovariectomy 24 Implantology Vol. 14, No

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3 original article 26 Implantology Vol. 14, No

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5 original article 28 Implantology Vol. 14, No

6 Öü Won Hee Kim et al: The effect of MBCP TM bone regeneration in osteoporosis induced rats. Implantology

7 original article 1. Özgür Erdogan DDS, David M. Shafer DMD, Pamela Taxel MA, MDand Martin A. Freilich DDS A Review of the association between osteoporosis and alveolar ridge augmentation. Oral Surg Oral Med Oral Pathol Oral Radiol Endod Dec;104(6):738.e1-13. Epub 2007 Jul Eddy DM, Johnston CC, Cummings SR, Dawson-Hughes B, Lindsay R, Melton LJ, Slemenda CW. Osteoporosis: Review of the evidence for prevention, diagnosis, treatment and cost-effectiveness analysis. Status report. Osteoporos Int 1998;4: Nilas L, Christiansen C. The pathophysiology of peri and postmenopausal bone loss. Br J Obstet Gynaecol 1989;96: Bae CS. Osteoporotic changes after ovariectomy in rats. Korean J Vent Clin Med 2000;17: von Wowern N, Klausen B, Kollerup G. Osteoporosis: A risk factor in periodontal disease. J Periodontol 1994;65: Hohlweg-Majert B, Schmelzeisen R, Pfeiffer BM, Schneider E. Significance of osteoporosis in craniomaxillofacial surgery: A review of the literature. Osteoporos Int 2006;17(2): White SC. Oral radiographic predictors of osteoporosis. Dentomaxillofac Radiol 2002;31(2): Kribbs PJ. Comparison of mandibular bone in normal and osteoporotic women. J Prosthet Dent 1990;63: Acarturk TO, Hollinger JO. Commercially available demineralized bone matrix compositions to regenerate calvarial critical-sized bone defects. Plast Reconstr Surg 2006 Sep 15;118(4): Schmitz JP, Hollinger JO. The critical size defect as an experimental model for craniomandibular nonunion. Clin Orthop Relat Res 1986;205: E.S. Elsubeihi, M.D. Grynpas, A.M. Cheung, G.A. Zarb and J.N.M. Heerche, The effect of ovariectomy on bone loss in the edentulous mandible and dentate mandible, Bone 28 (5) 2001; Shui C, Scutt A. Mild heat shock induces proliferation alkaline phosphatase activity and mineralization in human bone marrow stromal cells and Mg-63 cells in vivo. J Bone Miner Res 2001;16: R. Z. LeGeros E. Nery and K. Lynch Kerebel Transformation of biphasic calcium phosphate ceramics in vivo: Ultrastructural and physicochemical characterization Journal of Biomedical Materials Research, 1989;Vol. 23, R. Z.legeros, S. Lin, R. Rohanieadeh, D. mijares, J.P legeros. Biphasic calcium phosphate bioceramics: preparation, properties, and application Journal of materials science: materials in medicine ; Bohner M, Gbureck U. & Barralet J. E. Technological issues for the development of more efficient calcium phosphate bone cements: a critical assessment. Biomaterials 2005;26: Tahsin O.A.,Jeffery O.H. Commercially available demineralixed bone matrix compositions to regenerate calvarial critical-sixed bone defect. Plast. Reconstr. Surg.2006 Sep 15;118(4): Implantology Vol. 14, No

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