01 Cloning AccuRapid Cloning Kit NEW 3 Gene Cloning Service 4 AccuPower Ligation PreMix 6 T4 DNA Ligase 7 Thermostable Thermus filiformis (Tfi) DNA Li

Size: px
Start display at page:

Download "01 Cloning AccuRapid Cloning Kit NEW 3 Gene Cloning Service 4 AccuPower Ligation PreMix 6 T4 DNA Ligase 7 Thermostable Thermus filiformis (Tfi) DNA Li"

Transcription

1 Cloning DNA Amplification DNA Preparation Cloning (Cloning Kit & Gene Cloning Service) Phone: Cloning (Ligase) Phone: (ext.4->2) DNA Amplification Phone: (ext.4->2) DNA Preparation Phone: (ext.4->2)

2 01 Cloning AccuRapid Cloning Kit NEW 3 Gene Cloning Service 4 AccuPower Ligation PreMix 6 T4 DNA Ligase 7 Thermostable Thermus filiformis (Tfi) DNA Ligase 8

3 AccuRapid Cloning Kit NEW Features and Benefits 30분반응으로빠르고정확한 cloning Insert에제한효소를처리하지않고 vector에원하는방향으로삽입가능 Insert 양말단 complementary sequence 를인식하여연결되므로, PCR primer 디자인을통해여러조각 (1~3 조각 ) 삽입가능 Cloning 디자인을다양화하여원하는형태로 vector 조작 Application AccuRapid TM Cloning Kit 는 1~3 조각의 insert (PCR product) 를선형화된 vector 에정확하고신속하게 cloning 할수있는제품입니다. 본제품은 PCR 로증폭된 insert 말단과선형화된 vector 양말단의 18 ~ 21 bp complementary sequence 를인식하여연결하는방법으로, 기존처럼 insert (PCR product 또는 plasmid) 에제한효소를처리한후 ligation 하는방법이아니므로 vector 에 insert 를원하는방향으로삽입할수있습니다. Insert 증폭을위한 PCR primer 는선형화된 vector 양말단의 18 ~ 21 bp complementary sequence 를추가하여쉽게디자인할수있습니다. Multiple fragment cloning Gene synthesis Gene cloning Mutagenesis Vector modification Fusion protein Procedure Cat. no. Product Description K-7110 AccuRapid TM Cloning Kit, 10 rxns K-7120 AccuRapid TM Cloning Kit, 20 rxns K-7130 AccuRapid TM Cloning Kit, 50 rxns 3

4 Gene Cloning Service 바이오니아의오랜경험과풍부한분자생물학기술로번거롭고많은시일이소요되는 cloning 및 sequence 확인과정을한번에해결해드립니다. Features and Benefits 빠르고경제적인서비스 - 유전자원재료부터초고속올리고합성기까지일괄생산체계구축으로빠르고저렴한서비스. Gene 합성국내최저가 정확한품질 - Automatic DNA sequencer(abi 3730) 를이용한 100% sequence guarantee Application 단백질기능향상원하는 vector와의 cloning을통해단백질의발현효율을높일수있습니다. 항체유전자제작각종질병진단을위한항체제작시발현하고자하는숙주에코돈을최적화함으로써발현효율을높일수있으며, 또한고역가항체를얻기위해 diversity를극대화한여러형태의항체라이브러리를손쉽게얻을수있습니다. 유용물질생산생물체개발각종산업유용물질을생산하는균주들의유용물질생산에관련된유전자들을최적화하여생산효율을향상시킬수있습니다. Procedure 의뢰가능 Materials 서비스비용추가비용소요기간부가서비스 Gene Cloning Service Plasmid, PCR product, Cultured E. coli cell 200,000원 /cloning ( ~ 1 kb insert, ~ 7 kb vector) * Bioneer에서제공하는 vector에 cloning 가능 Cultured cell 이용시 50,000원추가 Insert 1 kb ~: 50,000원 /500 bp 7 ~ 10 kb: 100,000원추가 Vector 10 kb ~: Inquire Low copy: Inquire 1 ~ 8 kb (vector+insert) Average 5 ~ 10 working days 8 ~ 11 kb (vector+insert) Average 10 ~ 15 working days 11 kb ~ (vector+insert) Inquire Plasmid 증량서비스 (100,000원/100 μg) * Low copy plasmid 서비스제외 E. coli cell stock 제공서비스 (100,000원) 유전자합성과병행할경우 (100,000원할인 ) 유전자의구조, 특성등에의해가격및기간이상승될수있습니다. Commercial vector 를이용하여 cloning service 이용시 vector 구입비용이별도로청구됩니다. Template DNA 송부시, plasmid DNA 150~200 ng/μl, vol. 10 μl 이상, purified PCR product 50 ng/μl, vol. 10μl 이상을보내주셔야합니다. Sample 을잘못보내주실경우, 총비용의 50% 가추가청구되오니확인후보내주시기바랍니다. 진행중취소할경우금액의 50% 청구됩니다. 의뢰한 Cloning Service 를부득이하게 hold 할경우, 최대 1 개월이며 1 개월이후에는자동으로주문이취소되고금액의 50% 가청구됩니다. 4

5 Gene Cloning Service 주문방법 1. 바이오니아홈페이지 ( Gene Synthesis Service 페이지에서온라인주문하기배너를클릭하셔서주문페이지로들어갑니다. 2. 주문에서주문양식파일을다운받아작성하신후 3. 의뢰하신서비스를분석한후에서비스정보 ( 최종가격 + 기간 ) 를이메일로송부해드립니다. 4. 서비스의뢰를최종결정하시면이메일 (geneorder@bioneer. co.kr) 로통보해주십시오. 5. 서비스시작알림메일을보내드리는시점부터서비스가시작됩니다. Template DNA 송부방법박스에소속기관과의뢰자명을정확히기입하시고, 아래의당사연계배송회사를통하여 sample 을배송해주세요. 당사연계택배회사 : 현대택배 보내실곳 : 대전시대덕구문평서로 8-11 ( 주 ) 바이오니아 Synthetic Biology 팀 (Tel: , 8515) 운송비 : template 개수에상관없이당사부담 ( 단, 연계택배회사외에다른택배이용시운송비는고객께서부담하셔야합니다.) 주문취소규정주문후 5 일이내취소시금액의 50% 가청구됩니다. 주문후 5 일이후취소시금액의 80% 가청구됩니다 * Mutagenesis/Gene Cloning Service 는 material 수령시점부터서비스가시작됩니다. 상담안내 Tel: ( 고객지원센터 ) 또는 , 8515 (Synthetic Biology 팀 ) genesynthesis-support@bioneer.com 상담시간 : 평일오전 9:00 ~ 오후 6:00 ( 주말, 휴일휴무 ) 5

6 AccuPower Ligation PreMix 짧은시간에상온에서 Ligation Application Cloning into vectors Library construction TA cloning Linker ligation Recirclization of linear DNA Experimental Data AccuPower Ligation PreMix 는 T4 DNA Ligase, ATP 및반응버퍼등 ligation reaction 수행에필요한구성성분을혼합하여 0.2 ml tube 에동결건조시킨제품으로, 실온에서 5 분 (blunt-end DNA 는 10 분 ) 이내에 cohesive-end DNA 를 plasmid vector 에고효율로 ligation 할수있습니다. 또한바이오니아가독자개발한안정화물질이첨가되어있어실온에서도활성이 4 개월동안유지되며냉동 (-20 ) 보관을하실경우에는 3 년이상활성이지속적으로유지됩니다. Figure 1. 반응시간에따른 AccuPower Ligation PreMix의효율성실험. Lane 1 ~ 8: Lambda DNA / Hind III fragment (1 μg) Lane 9 ~ 16: Lambda DNA / EcoR V fragment (1 μg) Lane 2, 10: 60분동안 ligation Lane 3, 11: 50분동안 ligation Lane 4, 12: 40분동안 ligation Lane 5, 13: 30분동안 ligation Lane 6, 14: 20분동안 ligation Lane 7, 15: 10분동안 ligation Lane 8, 16: 5분동안 ligation Features and Benefits Fast Reaction Time: 실온에서 cohesive-end DNA 는 5 분, blunt-end DNA 는 10 분이내에 ligation 이가능합니다. Stability: 첨가된안정화제에의해실온에서는 4 개월, -20 에서는 3 년이상효소의활성이유지됩니다. Ease-of-use: T4 DNA Ligase, ATP, 최적화된반응버퍼를 1 회분량씩 0.2 ml tube 에포함하고있는제품으로 vector 와 insert DNA 만첨가해주면됩니다. Reproducibility: 재현성있는결과를위해바이오니아의전제품은엄격한 ISO 품질시스템하에서생산됩니다. Figure 2. 타사제품과의 ligation 효율비교. Lane 1, 9: Intact Lambda DNA (1 μg) Lane 2 ~ 8: Lambda DNA / Hind III fragment (1 μg) Lane 10 ~ 16: Lambda DNA / EcoR V fragment (1 μg) 3, 4, 11, 12: Bioneer AccuPower Ligation PreMix 5, 13: Company N T4 DNA ligase 6, 14: Company N Quick Ligation Kit 7, 15: Company P LigaFast Rapid DNA ligation system 8, 16: Company A Ready-To-Go T4 DNA ligase Cat. no. Product Description K-7103 AccuPower Ligation PreMix, 96 tubes, 0.2 ml 8-tube strips, 20 μl reaction 6

7 T4 DNA Ligase For Ligation of DNA, TA Cloning, and Other Recombinant DNA Applications Reagents Supplied 10 x Reaction Buffer: 500 mm Tris-HCI (ph 7.8), 100 mm MgCl 2, 50 mm DTT, 10 mm ATP, 25 μg/ml BSA Concentration 20,000 U (200 U/μl) Storage Conditions 50% glycerol containing 10 mm Tris-HCI (ph 7.5), 50 mm KCI, 1 mm EDTA, 10 mm 2-mercaptoethanol 본제품은유전자재조합실험에서 DNA 사이의결합에사용됩니다. T4 DNA ligase 는 duplex DNA 또는 RNA 에있는 5 - 인산기말단과 3 - 수산기말단부위사이에 phosphodiester bond 형성을촉진하여 duplex DNA, RNA, 또는 DNA/RNA hybrids 에있는단일가닥틈사이를 repair 할뿐만아니라 blunt-end 그리고 cohesive-end 끝말단부위로부터 DNA 를연결시킵니다. Features and Benefits Storage Temperature -20 Unit Definition 0.01 Weiss unit of enzyme is defined as the amount of enzyme required to give 90% ligation of Hind III fragments of lambda DNA in 30 min, at 16 in 20 μl of the assay mixture. Experimental Data High Speed: cohesive end DNA에서 25, 10분, blunt end DNA에서 25, 10분반응으로 DNA ligation이가능합니다. Flexibility: 대부분의 DNA ligation에최적화된제품입니다. Reproducibility: 재현성있는결과를위해바이오니아의전제품은엄격한 ISO 품질시스템하에서생산됩니다. Specifications Heat Inactivation: 70 for 10 min Application Blunt or cohesive-end ligation Repair of nicks in double-stranded nucleic acids Figure 1. T4 DNA Ligase를이용한 ligation. Lane 1: DNA fragment (digested with EcoR V) Lane 2, 3, 4, 5: T4 DNA Ligase 1 U, 16, 10, 20, 30 and 60 min Lane 6, 7, 8, 9: T4 DNA Ligase 1 U, 25, 10, 20, 30 and 60 min Lane 10, 11, 12, 13: T4 DNA Ligase 1 U, 37, 10, 20, 30 and 60 min Lane 14: Lambda DNA (digested with Hind III) Lane 15, 16, 17: T4 DNA Ligase 1 U, 16, 10, 20 and 30 min Lane 18, 19, 20: T4 DNA Ligase 1 U, 25, 10, 20 and 30 min Lane 21, 22, 23: T4 DNA Ligase 1 U, 37, 10, 20 and 30 min Cat. no. Product Description E-3061 T4 DNA Ligase, 20,000 U, 1 tube E-3062 T4 DNA Ligase, 100,000 U, 20,000 U x 5 tubes 7

8 Thermostable Thermus filiformis (Tfi) DNA Ligase Unit definition One unit of Tfi DNA Ligase is defined as the amount of enzyme required to give 50% ligation of the 12 base pair cohesive ends of 1 μg of PspE I digested lambda DNA in 10 min at 45. Tfi DNA Ligase는 DNA Ligase 의일종으로서절단된이중나선DNA 분자의인접한 5 -인산기말단과 3 -수산기말단사이를인산디에스테르결합 (phosphodiester bond) 으로연결해주는효소입니다. 특히다른 T4 DNA ligase, E. coli DNA ligase 등에비해더높은온도에서활성이안정하게유지되므로높은온도 (45~65 ) 에서반응할수있어반응시간이단축되고반응특이성이향상되었습니다. Source: Tfi DNA Ligase is isolated from E. coli cells containing the ligase gene cloned from Thermus filiformis. Application Ligase Chain Reaction (LCR) Oligonucleotide Ligation Assay (OLA) Mutagenesis by Incorporation of a phosphorylated oligo during PCR Amplification Simultaneous Mutagenesis of Multiple Sites Activity Assay Conditions The activity assay is carried out in a 20 μl reaction containing 1 μg of PspE I digested lambda DNA and 1 x Tfi DNA ligase reaction buffer. After incubation at 45 for 10 min., the reaction is terminated by addition of stop solution (40%(w/v) sucrose, 50 mm EDTA and 0.25% bromophenol blue). Then heat at 70 for 10 min and immediately load on a 0.8% agarose gel. Stability The half-life of the enzyme in 1 x reaction buffer is more than 1 hr at 95 and 55 hr at 65. Note: Tfi DNA Ligase should not be used as a substitute for other DNA ligase, i.e., T4 DNA Ligase. References Barany, F. (1991) Proc. Natl. Acad. Sci. USA, 88, Landegren, U. et al.(1988) Science 241, Michael, Scott F. (1994) Biotechniques 16:3, Gerard J. A. et al. (1993) Biotechniques 15:1, Reagents Supplied 10 x Reaction buffer (1 ml): 300 mm Tris-HCl (ph 8.3), 250 mm KCl, 50 mm MgCl 2, 5 mm NAD 1 x Dilution buffer (1 ml): 10 mm Tris-HCl (ph 7.6), 0.1 mm EDTA, 50 mm KCl, 1 mm DTT, 200 μg/ml acetylated BSA, 50% Glycerol Storage Condition 20 mm Tris-HCl (ph 7.6), 2 mm MgCl 2, 1 mm EDTA, 1 mm DTT, 0.5% Tween -20, 0.5% IGEPAL CA-630, 50% Glycerol, store at -20. Concentration 20 units/μl 8

9 Thermostable Thermus filiformis (Tfi) DNA Ligase Experimental Data Heat Stability test at 95 C and 65 C Fragment 4. Fragment Ligation Product (1+4) Figure 1. Ligation test at various temperatures (45 ~65 ) Incubate the reaction containing ligase 1 unit and 1 μg DNA[lambda PspE I] at each temperature for 10 min. Lane 1: λdna/pspe I (control) Lane 2: Incubate at 45, 10 min Lane 3: Incubate at 50, 10 min Lane 4: Incubate at 55, 10 min Lane 5: Incubate at 60, 10 min Lane 6: Incubate at 65, 10 min Figure 2. Heat Stability test at 95. Incubate the enzyme at 95 each time. And then add 1 unit ligase to a 20 μl reaction containing 1 μl DNA[lambda PspE I] and incubate the mixture at 45 for 10 min. Lane 1: λdna/pspe I (control) Lane 2: Incubate at 95, 10 min Lane 3: Incubate at 95, 20 min Lane 4: Incubate at 95, 30 min Lane 5: Incubate at 95, 40 min Lane 6: Incubate at 95, 50 min Lane 7: Incubate at 95, 60 min Lane 8: Incubate at 95, 70 min Lane 9: Incubate at 95, 80 min Lane 10: Incubate at 95, 90 min Cat. no. Product Description E-3111 Tfi DNA Ligase, 2,000 U, 10 x reaction buffer, 1 ml, 1 x dilution buffer, 1 ml E-3112 Tfi DNA Ligase, 10,000 U, 10 x reaction buffer, 1 ml, 1 x dilution buffer, 1 ml 9

10 02 DNA Amplification AccuPower Pfu PCR PreMix 11 AccuPower ProFi Taq PCR PreMix 13 AccuPower HotStart Pfu PCR PreMix 15 Pfu DNA Polymerase 17 ProFi Taq DNA polymerase 19

11 AccuPower Pfu PCR PreMix Gene Cloning 등 High Fidelity 를요구하는실험에최적화된 Kit AccuPower Pfu PCR PreMix 는 proof-reading 기능을갖는 Pfu DNA polymerase 를사용하여매우정확한 PCR product 을얻을수있는제품입니다. 또한, Pfu DNA polymerase 와 PCR 반응혼합물이동결건조되어제공되므로 template DNA 와 primers 의첨가만으로간편하게 high fidelity 를갖는 PCR product 를얻을수있습니다. Specifications Enzyme: Pfu DNA polymerase 5 to 3 exonuclease activity: No 3 to 5 exonuclease activity: Yes 3 - A overhang: No Fragment size: ~ 15 kb Application Gene synthesis Gene cloning Conventional PCR Primer extension Site directed mutagenesis High fidelity 가요구되는실험 Storage Temperature -20 Features and Benefits High Fidelity: 높은정확성 (error rate= 1.9X10-6 ) 을가지고있어 DNA 증폭시발생하는 mutation 을최소화하였습니다. Sensitivity: 높은민감도와증폭효율로미량의 human gdna target 을검출합니다. Long Range PCR: Human gdna 의경우 15 kb 이상의 PCR 를효과적으로증폭시킬수있습니다. Ease-of-use: 각각의 PCR tube 에 DNA polymerase 와 PCR 수행에필요한모든구성성분이포함되어있어 template DNA, primer set, D.W. 만넣어바로 PCR 반응을수행할수있습니다. 또한전기영동시에필요한 tracking dye 와침강제가포함되어있어 sample loading buffer 를첨가할필요가없으므로간편하게사용할수있습니다. Stability: PCR 반응혼합물에안정화제를첨가하여건조시켜, 장기간보관하더라도활성을안정적으로유지합니다. Reproducibility: ISO 9001 품질시스템하에서 one-batch system 으로대량생산되어각 batch 에대한철저한 QC 를거친후균일한품질의제품으로공급되기때문에사용자가대량의시료를반복적으로처리할때각 tube 마다발생하는편차문제를해결하여재현성있는실험결과를제공합니다. 11

12 AccuPower Pfu PCR PreMix Experimental Data Figure 1. Template range & sensitivity of AccuPower Pfu PCR PreMix for human DNA template. Test of working range & sensitivity of AccuPower Pfu PCR PreMix for human DNA template. Line 1: 100 ng Line 2: 10 ng Line 3: 1 ng Line 4: 100 pg Line 5: 10 pg Line 6: Template negative M: 100 bp DNA Ladder (Bioneer, Cat. no. D-1030) Figure 2. Amplification of lambda DNA of 1 kb to 10 kb with AccuPower Pfu PCR PreMix. Lane 1: 1 kb fragment Lane 2: 2 kb fragment Lane 3: 3 kb fragment Lane 4: 4 kb fragment Lane 5: 5 kb fragment Lane 6: 6 kb fragment Lane 7: 7 kb fragment Lane 8: 8 kb fragment Lane 9: 9 kb fragment Lane 10: 10 kb fragment M: 1 kb DNA Ladder (Bioneer, Cat. no. D-1040) Cat. no. Product Description K-2022 AccuPower Pfu PCR PreMix, 0.2 ml thin-wall 8-tube strips with attached cap / 96 tubes, 20 μl rxn K-2023 AccuPower Pfu PCR PreMix, 0.2 ml thin-wall 8-tube strips with attached cap / 96 tubes, 50 μl rxn K-2024 AccuPower Pfu PCR PreMix, 0.2 ml thin-wall 8-tube strips with attached cap / 480 tubes, 20 μl rxn K-2025 AccuPower Pfu PCR PreMix, 0.2 ml thin-wall 8-tube strips with attached cap / 480 tubes, 50 μl rxn K-2026 AccuPower Pfu PCR Master Mix, 1 ml of 2X master mix solution K-2027 AccuPower Pfu PCR PreMix, 0.5 ml thin-wall tubes with attached cap / 100 tubes, 50 μl rxn 12

13 AccuPower ProFi Taq PCR PreMix High Efficiency 및 Long Range PCR 에최적화된 Kit Specifications Enzyme: ProFi Taq DNA polymerase 5 to 3 exonuclease: Yes 3 to 5 exonuclease: Yes 3 - A overhang: Yes PCR product size: ~ 30 kb AccuPower ProFi Taq PCR PreMix 는바이오니아에서독자적으로개발한 ProFi Taq DNA polymerase 와 PCR 반응혼합물이진공건조된제품으로, template DNA, primer 그리고 D.W. 의첨가만으로정확하고깨끗한 PCR 산물을얻을수있는제품입니다. 본제품에사용되는 ProFi Taq DNA polymerase 는높은정확성과뛰어난증폭효율을나타내며, Human Genomic DNA 의경우최대 21 kb (Lambda DNA 의경우최대 30 kb) 의 DNA fragment 증폭이가능합니다. AccuPower ProFi Taq PCR PreMix 는 high efficiency 및 longrange PCR 에매우적합한제품이며, 그외에도다양한 PCR 에사용하실수있습니다. Features and Benefits Long Range PCR: Lambda DNA 의경우 30 kb, human gdna 의경우 21 kb 의 PCR 산물을효과적으로증폭할수있습니다. Sensitivity: 뛰어난민감도와증폭효율로미량의 human gdna target 을검출합니다. 타사제품과의비교실험을통해우수한민감도를확인했습니다. Ease-of-use: 각각의 PCR tube 에 ProFi Taq DNA polymerase 와 PCR 반응에필요한모든구성성분이포함되어있어 template DNA, primer set 와 D.W. 만넣어바로 PCR 반응을수행할수있습니다. 또한전기영동시에필요한 tracking dye 와침강제가포함되어있어별도로 sample loading buffer 를첨가할필요가없으므로간편하게사용할수있습니다. Application Primer extension Long range amplification from genomic DNA High amplification efficiency Excellent performance on difficult templates Amplification of low-copy targets High yield and high sensitivity PCR Storage Temperature -20 Experimental Data M Supplier I Supplier S Supplier T Figure 1. Comparison of PCR amplification efficiency between AccuPower ProFi Taq PCR PreMix from Bioneer and other suppliers PCR master mix. cdna synthesized from 10-fold serial-diluted human total RNA from 10 ng to 10 pg using AccuPower RocketScript TM Cycle RT PreMix(Bioneer, Cat. no. K-2201) was used as a template for PCR amplification. The cycling conditions for AccuPower ProFi Taq PCR PreMix were 95 for 5 min, 33 cycles of 95 for 20 sec, 55 for 20 sec and 72 for 30 sec. PCR reactions using other suppliers PCR master mix were performed according to each supplier s protocol. Target: human GAPDH gene Lane 1: 10 ng of human total cdna Lane 2: 1 ng of human total cdna Lane 3: 100 pg of human total cdna Lane 4: 10 pg of human total cdna Lane M: 100 bp DNA Ladder (Bioneer, Cat. no. D-1030) Stability: PCR 반응혼합물에안정화제를첨가하여건조시켜, 장기간보관하더라도활성을안정적으로유지합니다. Reproducibility: ISO 9001 품질시스템하에서 one-batch system 으로대량생산되어각 batch 에대한철저한 QC 를거친후균일한품질의제품으로공급되기때문에사용자가대량의시료를반복적으로처리할때각 tube 마다발생하는편차문제를해결하여재현성있는실험결과를제공합니다. 13

14 AccuPower ProFi Taq PCR PreMix M Supplier I Supplier S Supplier T M M1M Supplier I Supplier S Supplier T M2M1 Figure 2. Comparison of PCR amplification sensitivity between AccuPower ProFi Taq PCR PreMix from Bioneer and other suppliers PCR master mix. The cycling conditions for AccuPower ProFi Taq PCR PreMix were 95 for 5 min, 30 cycles of 95 for 20 sec, 65 for 20 sec and 68 for 4 min. PCR reactions using other suppliers PCR master mix were performed according to each supplier s protocol. Lane 1: 2 kb fragment (human tumor protein p53 gene) Lane 2: 3 kb fragment (human tumor protein p53 gene) Lane 3: 4.5kb fragment (human DNA cross-link repair 1A gene) Lane 4: 8 kb fragment (human hemoglobin epsilon 1 gene) Lane M: 1 kb DNA Ladder (Bioneer, Cat. no. D-1040) Figure 3. Comparison of PCR amplification of long targets between AccuPower ProFi Taq PCR PreMix from Bioneer and other suppliers PCR master mix. The cycling conditions for AccuPower ProFi Taq PCR PreMix were 95 for 5 min, 32 cycles of 95 for 20 sec, 65 for 40 sec, and 68 for 15 min. PCR reactions using other suppliers PCR master mix were performed according to each supplier s protocol. Human DNA was used as a template for PCR amplification. Lane 1: 11 kb fragment Lane 2: 13.5 kb fragment Lane 3: 17.6 kb fragment Lane 4: 21.4 kb fragment Lane M1: Lambda/Hind III marker (Bioneer, Cat. no. D-1050) Lane M2: 1 kb DNA Ladder (Bioneer, Cat. no. D-1040) Cat. no. Product Description K-2631 AccuPower ProFi Taq PCR PreMix, 0.2 ml thin-wall 8-tube strips with attached caps / 96 tubes, 20 μl rxn/tube K-2632 AccuPower ProFi Taq PCR PreMix, 0.2 ml thin-wall 8-tube strips with attached caps / 480 tubes, 20 μl rxn/tube K-2633 AccuPower ProFi Taq PCR PreMix, 0.2 ml thin-wall 8-tube strips with attached caps / 96 tubes, 50 μl rxn/tube K-2634 AccuPower ProFi Taq PCR PreMix, 0.2 ml thin-wall 8-tube strips with attached caps / 480 tubes, 50 μl rxn/tube 14

15 AccuPower HotStart Pfu PCR PreMix Gene Cloning 등 High Fidelity 를요구하는실험에최적화된 Kit Specifications Enzyme: Pfu DNA polymerase 5 to 3 exonuclease activity: No 3 to 5 exonuclease activity: Yes 3 - A overhang: No Fragment size: ~ 10 kb Application Gene cloning with blunt ends Site-directed mutagenesis High fidelity amplification High specificity PCR cdna template PCR AccuPower HotStart Pfu PCR PreMix 는세계적으로인정받은바이오니아특허기술인 enzyme-mediated HotStart 기술이적용되어 miss-priming, primer dimer, non-specific amplification 을근본적으로제거할수있어서미량의 target DNA 도정확하게증폭해낼수있는제품입니다. 또한본제품에포함된 Pfu DNA polymerase 는 proof-readiing (3 5 exonuclease) 기능을가지고있어서 DNA 증폭반응시발생하는 error 를줄일수있어단백질생산을위한 cloning 실험에적합합니다. Storage Temperature -20 Experimental Data Features and Benefits High Fidelity: Proof-reading 기능과높은특이성을가지고있어 cloning 에가장적합한제품입니다. Ease-of-use: 각각의 PCR tube 에 DNA polymerase 와 PCR 수행에필요한모든구성성분이포함되어있어 template DNA, primer set, D.W. 만넣어바로 PCR 반응을수행할수있습니다. 또한전기영동시에필요한 tracking dye 와침강제가포함되어있어 sample loading buffer 를추가로넣을필요가없으므로간편하게사용할수있습니다. Specificity: Pfu DNA polymerase 의 proof-reading 기능을최대한살리면서 enzyme-mediated HotStart 의특장점을통해 cloning, mutagenesis 등정확한 sequence 가요구되는실험에서최적의 PCR product 를얻을수있습니다. Stability: PCR 반응혼합물에안정화제를첨가하여건조시켜, 장기간보관하더라도활성을안정적으로유지합니다. Figure 1. AccuPower HotStart Pfu PCR PreMix shows enhanced specificity compared to competitors. Specificity test was performed using 7 different sets of primers targeting the p53 gene. 10 ng of human genomic DNA was used for each PCR reaction. The cycling conditions were 95 for 5 min, 32 cycles of 95 for 30 sec, 62 for 40 sec, and 72 for 1 min 30 sec, and 72 for 5 min for final extension. Lane 1: P75/73 primer set (139 bp) Lane 2: P55/53 primer set (211 bp) Lane 3: P55/63 primer set (447 bp) Lane 4: P75/83 primer set (618 bp) Lane 5: P55/73 primer set (1082 bp) Lane 6: P65/83 primer set (1296 bp) Lane 7: P55/83 primer set (1561 bp) Lane M: 100 bp DNA Ladder (Bioneer, Cat. no. D-1030) Reproducibility: ISO 9001 품질시스템하에서 one-batch system 으로대량생산되어각 batch 에대한철저한 QC 를거친후균일한품질의제품으로공급되기때문에사용자가대량의시료를반복적으로처리할때각 tube 마다발생하는편차문제를해결하여재현성있는실험결과를제공합니다. 15

16 AccuPower HotStart Pfu PCR PreMix Figure 2. AccuPower HotStart Pfu PCR PreMix has high amplification efficiency. Bioneer reaction mixture was followed by 95 for 5 min, 35 cycles of 95 for 20 sec, 65 for 20 sec, and 68 for 15 min, and 68 for 5 min for final extension. Lane 1: 2 kb fragment Lane 2: 2.5 kb fragment Lane 3: 3 kb fragment Lane 4: 4.5 kb fragment Lane 5: 5 kb fragment Lane M: 1 kb DNA Ladder (Bioneer, Cat. no. D-1040) Figure 3. Comparison of PCR amplification efficiency between AccuPower HotStart Pfu PCR PreMix from Bioneer and other suppliers PCR master mix Target: human insulin receptor gene. The cycling conditions for AccuPower HotStart Pfu PCR PreMix were 95 for 5 min, 30 cycles of 95 for 30 sec, 55 for 30 sec and 72 for 2 min. PCR reactions using other suppliers PCR master mix were performed according to each supplier s protocol. Lane 1: 10 ng of human genomic DNA Lane 2: 1 ng of human genomic DNA Lane 3: 100 pg of human genomic DNA Lane 4: 10 pg of human genomic DNA Lane M: 100 bp DNA Ladder (Bioneer, Cat. no. D-1030) Cat. no. Product Description K-2301 AccuPower HotStart Pfu PCR PreMix 96 tubes, 20 μl rxn K-2302 AccuPower HotStart Pfu PCR PreMix 96 tubes, 50 μl rxn K-2303 AccuPower HotStart Pfu PCR PreMix 480 tubes, 20 μl rxn K-2304 AccuPower HotStart Pfu PCR PreMix 480 tubes, 50 μl rxn 16

17 Pfu DNA Polymerase Novel Enzyme for High Fidelity PCR with DNA Proofreading Specifications 5 to 3 exonuclease activity: No 3 to 5 exonuclease activity: Yes 3 -A overhang: No Fragment size: ~10 kb Application Gene synthesis PCR or Primer extension requested high fidelity Blunt-end PCR Cloning or mutagenesis requested high fidelity Pfu DNA Polymerase 는 Pyrococus furiosus 라는 bacteria 에서유래되었으며, 3 5 exonuclease (proofreading) activity 를지니고있어기존의 Taq DNA Polymerase 보다 heat stability 와 fidelity 가뛰어납니다. 또한뛰어난 specificity 를가지고있어 nonspecific product 의발생이적으며, proofreading 기능이있어 DNA 증폭시발생하는 error rate 을감소시킵니다. Pfu DNA Polymerase 는 gene cloning, PCR, primer extension 등다양한실험에사용하실수있습니다. Features and Benefits High Fidelity PCR: 3 5 proofreading activity 가뛰어납니다. Thermostability: 열안정성이뛰어나 95 에서 1 시간반응후에도 94~99% 의기능을유지합니다. Terminal Transferase Activity: Terminal transferase activity 가없어 blunt-ended PCR product 를얻을수있습니다. Reagents Supplied 10 x Reaction Buffer: 300 mm Tris-HCl, 200 mm KCl, 100mM (NH 4) 2SO 4, 15 mm MgSO 4, Acetylated BSA, ph 9.0 Dilution buffer: 50 mm Tris-HCl, 0.1 mm EDTA, 1 mm DTT, Stablizers, 50% Glycerol, ph 8.0 dntps mixture: 10 mm, each dntp 2.5 mm (optional) Concentration 250 U (2.5 U/μl) Storage Temperature -20 Unit Definition One unit is defined at the amount of enzyme that will incorporate 10 nmol of dntp into acid-insoluble material in 30 min at 72. Reproducibility: 재현성있는결과를위해바이오니아의전제품은엄격한 ISO 품질시스템하에서생산됩니다. 17

18 Pfu DNA Polymerase Experimental Data M M Figure 1. Human DNA was amplified using 2.5 units of enzyme in 50 μl reaction volume. Lane 1: 20 ng Lane 2: 2 ng Lane 3: 200 pg Lane 4: 20 pg M: 100 bp DNA ladder (Bioneer, Cat. no. D-1030) Figure 2. Lambda DNA 를이용한 Pfu DNA Polymerase 의 Long kb PCR test. Lane 1: Lambda DNA 5 kb Lane 2: Lambda DNA 6 kb Lane 3: Lambda DNA 7 kb Lane 4: Lambda DNA 8 kb M: 1 kb DNA ladder (Bioneer, Cat. no. D-1040) Cat. no. Product Description E-2015 Pfu DNA Polymerase, 250 U, 10 x reaction buffer E Pfu DNA Polymerase, 250 U, 10 mm dntps, 10 x reaction buffer E-2016 Pfu DNA Polymerase, 1,000 U, 10 x reaction buffer 18

19 ProFi Taq DNA Polymerase For High Efficiency and Amplification of Long Range PCR. Specifications 5 to 3 exonuclease activity: Yes 3 to 5 exonuclease activity: Yes 3 -A overhang: Yes Fragment size: ~30 kb Application Primer extension long-range amplification from genomic DNA High amplification efficiency Excellent performance on difficult templates Amplification of low-copy targets High yield and high sensitivity PCR ProFi Taq DNA Polymerase 는합성생물학기술을이용하여개발한 recombinant Taq DNA polymerase 로써뛰어난증폭효율과정확성을동시에충족시키는 DNA polymerase 입니다. ProFi Taq DNA polymerase 는 Taq DNA polymerase 를개선한효소로써기존의 Taq DNA polymerase 와비교하여뛰어난증폭성능, 높은 efficiency 를가지는제품입니다. ProFi Taq DNA Polymerase 는 human genomic DNA 의경우 21 kb 까지 DNA fragment 증폭이가능하며 lambda DNA 의경우 30 kb 까지 DNA fragment 증폭이가능합니다. ProFi Taq DNA Polymerase 는 high efficiency 및 long range PCR 에매우적합한제품이며그외에도 complex genomic DNA 또는 cdna templates, low-copy targets 등다양한 PCR 증폭반응에사용하실수있습니다. Reagents Supplied 10 x Reaction Buffer: 400 mm Tris-HCl, 600 mm KCl, 15 mm MgCl 2, Acetylated BSA, ph 9.0 Dilution buffer: 20 mm Tris-HCl, 0.5 mm EDTA, 1 mm DTT, 100 mm KCl, Stablizers, 50% Glycerol, ph 8.0 dntps mixture: 10 mm, each dntp 2.5 mm Concentration 250 U (5 U/μl) Storage Temperature -20 Features and Benefits High Sensitivity: 뛰어난민감도와증폭효율로미량의 human gdna target을검출합니다. 타사제품과의비교실험을통해우수한민감도및증폭효율을가집니다. Long Range PCR: Lambda DNA의경우 30 kb, human genomic DNA의경우 21 kb의 PCR 산물을효과적으로증폭할수있습니다. Reproducibility: 재현성있는결과를위해바이오니아의전제품은엄격한 ISO 품질시스템하에서생산됩니다. 19

20 ProFi Taq DNA Polymerase Experimental data Bioneer M Supplier T Supplier S Supplier I Figure 1. Comparison of PCR amplification efficiency between ProFi Taq DNA Polymerase from Bioneer and other suppliers DNA polymerase. The cycling conditions for ProFi Taq DNA Polymerase were 95 for 5 min, 30 cycles of 95 for 20 sec, 55 for 20 sec and 72 for 30 sec. PCR reaction using other suppliers DNA polymerase were performed according to each supplier s protocol. Target: human Insulin receptor gene Lane 1: 10 ng of human genomic DNA Lane 2: 1 ng of human genomic DNA Lane 3: 100 pg of human genomic DNA Lane 4: 10 pg of human genomic DNA Lane M: 100 bp DNA Ladder(Bioneer, Cat. no. D-1030) Bioneer M1 M Supplier I Supplier S Supplier T M2 M1 Figure 2. Comparison of PCR amplification of long targets between ProFi Taq DNA Polymerase from Bioneer and other suppliers DNA polymerase The cycling conditions for ProFi Taq DNA Polymerase were 95 for 5 min, 32 cycles of 95 for 20 sec and 68 for 15 min. PCR reactions using other suppliers DNA polymerase were performed according to each supplier s protocol. Human genomic DNA was used as a template for PCR amplification. Lane 1: 11 kb fragment Lane 2: 13.5 kb fragment Lane 3: 17.6 kb fragment Lane 4: 21.4 kb fragment Lane M1: Lambda/Hind III marker (Bioneer, Cat. no. D-1050) Lane M2: 1 kb DNA Ladder (Bioneer, Cat. no. D-1040) M1M Supplier I Supplier S Supplier T M2M1 Figure 3. Comparison of PCR amplification of long targets between ProFi Taq DNA Polymerase from Bioneer and other suppliers DNA polymerase. The cycling conditions for ProFi Taq DNA Polymerase were 95 for 5 min, 30 cycles of 95 for 20 sec, 65 for 20 sec, and 68 for 4 min. PCR reactions using other suppliers DNA polymerase were performed according to each supplier s protocol. Lambda DNA was used as a template for PCR amplification. Lane 1: 15 kb fragment (human tumor protein p53 gene) Lane 2: 20 kb fragment (human tumor protein p53 gene) Lane 3: 25 kb fragment (human DNA cross-link repair 1A gene) Lane 4: 30 kb fragment (human hemoglobin epsilon 1 gene) Lane M1: Lambda/Hind III marker (Bioneer, Cat. no. D-1050) Lane M2: 1 kb DNA Ladder (Bioneer, Cat. no. D-1040) Cat. no. Product Description E-2201 ProFi Taq DNA Polymerase 250 U, 10 mm dntps, 10 X reaction buffer with MgCl 2 E-2202 ProFi Taq DNA Polymerase 250 U, 10 mm dntps, 10 X reaction buffer without MgCl 2, 20 mm MgCl 2 E-2203 ProFi Taq DNA Polymerase 250 U, 10 X reaction buffer with MgCl 2 E-2204 ProFi Taq DNA Polymerase 250 U, 10 X reaction buffer without MgCl 2, 20 mm MgCl 2 E-2205 ProFi Taq DNA Polymerase 1000 U, 10 mm dntps, 10 X reaction buffer with MgCl 2 E-2206 ProFi Taq DNA Polymerase 1000 U, 10 mm dntps, 10 X reaction buffer without MgCl 2, 20 mm MgCl 2 E-2207 ProFi Taq DNA Polymerase 1000 U, 10 X reaction buffer with MgCl 2 E-2208 ProFi Taq DNA Polymerase 1000 U, 10 X reaction buffer without MgCl 2, 20 mm MgCl 2 20

21 03 DNA Preparation Magnetic Bead Type Kit MagListo 5M Plasmid Extraction Kit 21 MagListo 5M PCR Purification Kit 23 MagListo 5M Gel Extraction Kit 24 Spin Column Type Kit AccuPrep Nano-Plus Plasmid Mini Extraction Kit 25 AccuPrep Plasmid Mini Extraction Kit 27 AccuPrep PCR Purification Kit 29 AccuPrep Gel Purification Kit 32

22 MagListo 5M Plasmid Extraction Kit Plasmid Mini, Midi and Maxi Prep 을한번에! 빠르고간편한신개념 Plasmid DNA 추출키트 Experimental Data MagListo TM 5M Plasmid Extraction Kit 은 Magnetic Nanobead 와 MagListo TM Magnetic Separation Rack 을이용하여 5 분 (mini), 10 분 (midi), 15 분 (maxi) 내에배양된박테리아세포로부터 plasmid DNA 를추출할수있는제품입니다. 자성분리방식으로세포분쇄물과 plasmid DNA 를분리, 농축및정제하는과정을거치기때문에원심분리기를사용하는방법에비해빠르게 plasmid DNA 를분리할수있습니다. 본제품은한 kit 를사용하여 mini, midi, maxi scale 로 plasmid DNA 를추출할수있어별도의 kit 를구매할필요가없으며, vacuum system 이나 air pressure system 을구비할필요가없어실험비용을절감할수있습니다. Figure 1. Electrophoresis data of 500 ng of several size plasmids purified with MagListo TM 5M Plasmid Extraction Kit. M1: Bioneer 100 bp ladder 1: 3.5 kb plasmid DNA 2: 5.4 kb plasmid DNA 3: 8 kb plasmid DNA M2: Bioneer 1 kb ladder Features and Benefits Magnetic Nanobead를이용하여 mini-5분, midi-10분, maxi- 15분에 plasmid 추출 Mini, midi, maxi prep kit을따로구매할필요없이하나의 kit 로해결 Extraction kit와별도의 MagListo TM rack 외에고가의추가장비불필요 Application Gene cloning, PCR, Real-Time PCR, sequencing, transformation, transfection, in vitro transcription/translation Specifications Figure 2. Comparison of plasmid DNAs purified using MagListo TM and competitors products (magnetic bead type). M1: Bioneer 100 bp ladder 1-2: Plasmid DNA purified with Bioneer MagListo TM (3.5 kb / 5 kb) 3-4: Plasmid DNA purified with competitor P kit (3.5 kb / 5 kb) 5-6: Plasmid DNA purified with competitor I kit (3.5 kb / 5 kb) M2: Bioneer 1 kb ladder 1(B) 2(B) 3(B) 4(B) 5(B) 6(B) Concentration(ng/ul) A260/ Mini scale Midi scale Maxi scale Starting culture volume 1~5 ml 20~50 ml 100~200 ml Elution volume 100 μl 500 μl 1 ml Expected DNA yield ~ 20 μg ~ 400 μg ~ 1 mg Preparation time ~ 5 min ~ 10 min ~ 15 min 22

23 MagListo 5M Plasmid Extraction Kit Figure 3. Comparison of plasmid transfection efficiency in 293T cells between MagListo TM and a competitor s product (spin column type). 293T cell 1x10 6 을이용한 transfection efficiency 확인결과, Bioneer MagListo TM 와경쟁사제품에서추출한 plasmid 모두 95% 이상의효율을확인할수있습니다. Figure 4. Comparison cell viability after transfected with prep samples by MagListo TM and a competitor s product (spin column type). 293T 세포주에 transfection 후 WST assay를시행한결과, plasmid DNA 400 ng/well (6 well plate) 농도까지 cell viability에영향을미치지않음을확인할수있습니다. Procedure Cat. no. Product Description K-3600 MagListo TM 5M Plasmid Extraction Kit, 500 rxn in mini K-3601 MagListo TM 5M Plasmid Extraction Kit, 100 rxn in mini 23

24 MagListo 5M PCR Purification Kit PCR Product 를비롯한다양한효소반응물의 Fragment DNA 정제를빠르고간편하게! Specifications Size range 100 bp ~ 10 kb Typical recovery 90 ~ 100% Expected purity A260/280 > 1.8, A260/230 > 1.6 Purification time ~ 4 min Application Subcloning, Sequencing, Labeling, DNA concentration and other molecular biological applications Experimental Data MagListo TM 5M PCR Purification Kit 는 Magnetic Nanobead 와 MagListo TM Magnetic Separation Rack 을이용하여 PCR product 를비롯한다양한효소반응물 ( 제한효소반응, A-tailing 반응, labeling 반응등 ) 에서 fragment DNA 를빠르게추출할수있는제품입니다. 자성분리방식을이용하여원심분리기를사용하는방식보다각종불순물 (dimer, salts, dntps, enzymes, mineral oil, ethidium bromide, dye, detergent 등 ) 들을손쉽고빠르게제거하여고순도의 fragment DNA 를정제할수있습니다. A B Features and Benefits MagListo TM Magnetic Separation Rack를이용하여 4분만에 DNA 정제 원심분리기등의고가장비불필요 Figure 1. Comparison of fragment DNA purified with MagListo TM PCR Purification Kit and control. A. C: Control 100 bp DNA Ladder (D-1030) 1-2: 100 bp DNA Ladder purified with MagListo TM 5M PCR Purification Kit B. C: Control 1 kb DNA Ladder (D-1040) 1-2: 1 kb DNA Ladder purified with MagListo TM 5M PCR Purification Kit Procedure Cat. no. Product Description K-3609 MagListo TM 5M PCR Purification Kit, 100 rxn in mini 24

25 MagListo 5M Gel Extraction Kit Agarose Gel 로부터 Fragment DNA 정제를빠르고간편하게! Specifications Size range 100 bp ~ 10 kb Typical recovery 90 ~ 100% Expected purity A260/280 > 1.8 Purification time ~ 15 min Application Subcloning, Sequencing, Labeling, DNA concentration and other molecular biological applications Experimental Data MagListo TM 5M Gel Extraction Kit 는 Magnetic Nanobead 와 MagListo TM Magnetic Separation Rack 을이용하여 TAE, TBE agarose gel 에서원하는 fragment DNA 를추출할수있는제품입니다. Gel Solubilization Buffer 에는 ph indicator 가함유되어있어최적의 binding 조건을잡을수있으며, 자성분리방식을이용하여원심분리기를사용하는방법에비해빠르게고순도의 fragment DNA 를정제할수있습니다. Features and Benefits Magnetic Nanobead를이용하여 15분만에 agarose gel로부터 DNA 정제 원심분리기등의고가의장비불필요 Figure 1. Comparison of fragment DNA from TBE agarose gel purified with MagListo TM 5M Gel Extraction Kit and competitor s kit (spin column type). M: Bioneer 1 kb ladder 1-2: 500 bp DNA purified with Bioneer MagListo TM 5M Gel Extraction Kit 3-4: 500 bp DNA purified with competitor Q kit 5: Control 500 bp DNA 6-7: 3 kb DNA purified with Bioneer MagListo TM 5M Gel Extraction Kit 8-9: 3 kb DNA purified with competitor Q kit 10: Control 3 kb DNA 11-12: 5 kb DNA purified with Bioneer MagListo TM 5M Gel Extraction Kit 13-14: 5 kb DNA purified with competitor Q kit 15: Control 5 kb DNA Procedure Cat. no. Product Description K-3607 MagListo TM 5M Gel Extraction Kit, 100 rxn in mini 25

26 AccuPrep Nano-Plus Plasmid Mini Extraction Kit Specifications Starting culture volume Column binding capacity Elution volume Expected DNA yield Preparation time 1 ml ~ 10 ml > 20 μg μl ~ 20 μg ~ 10 min Application Subcloning, Sequencing, Transformation, Transfection, In vitro transcription/translation AccuPrep Nano-Plus Plasmid Mini Extraction Kit 는단 10 분내에고순도, 고농도의 plasmid DNA 를추출할수있는제품으로써 Birnboim 의 alkaline lysis method 와바이오니아의독창적인세포잔해입자및단백질제거특허기술이결합된신개념제품입니다. 본제품에포함된나노입자는용액내 insoluble protein aggregate 및 chromosomal DNA, cell debris 등과함께 complex 를형성하여원심분리시매우빠른시간내에 precipitate 를형성할수있게해줍니다. 또한, 용액내포함된나노입자에의해 insoluble complex ( 나노입자 + protein aggregate + chromosomal DNA + cell debris) 가매우단단하게 tube 벽면에고정됨으로써기존실험방법에비해고순도의 cleared lysate 를얻을수있습니다. 본제품은 cleared lysate 를얻기위해수행하던원심분리시간을획기적으로단축시켜 (10 분 1 분 ) 단 10 분이내에전체실험진행이가능합니다. 또한, DNA binding capacity 가극대화된 silica based DNA binding column 을적용하여높은수율을보장합니다. Experimental Data Figure 1. Reduced total preparation time. B: AccuPrep Nano-Plus Plasmid Mini Extraction Kit Q, P: Competitors s plasmid extraction kit Features and Benefits 바이오니아의독창적세포잔해입자및단백질제거특허기술이도입된신개념 plasmid DNA 추출키트 단 10분이내에 E. coli cell로부터고순도, 고수율의 plasmid DNA 추출 High-copy 및 low-copy plasmid DNA에도모두사용가능 enda+ strain을위한 Endonuclease A denaturation buffer 제공 (Denaturation Buffer, PD) 높은 binding capacity를갖는 silica based DNA binding column 사용 Figure 2. Restriction enzyme digestion test. AccuPrep Nano-Plus Plasmid Mini Extraction Kit를이용하여추출한 plasmid DNA를여러종류의제한효소로 digestion한후 agarose gel에서확인하였습니다. Left- pbluescript SK(+) Right- pbi121 Lane M: Size marker(cat. no. D-1040, Bioneer) 26

27 AccuPrep Nano-Plus Plasmid Mini Extraction Kit Procedure Cat. no. Product Description K-3111 AccuPrep Nano-Plus Plasmid Mini Extraction Kit, 200 rxn K-3112 AccuPrep Nano-Plus Plasmid Mini Extraction Kit, 50 rxn KB-0101 RNase A powder, lyophilized (6 mg/tube) KA DNA Binding Column Tubes (50 ea X 4 box) 27

28 AccuPrep Plasmid Mini Extraction Kit Specifications Starting culture volume Column binding capacity Elution volume Expected DNA yield Preparation time 1 ml ~ 10 ml > 20 μg 50 ~ 100 μl ~ 20 μg ~ 20 min Application Subcloning, Sequencing, Transformation, Transfection, In vitro transcription/translation AccuPrep Plasmid Mini Extraction Kit 는고순도, 고농도의 plasmid DNA 를추출할수있도록개발된제품으로써 Birnboim 의 alkaline lysis method 에기초한제품입니다. 본제품은높은 DNA binding capacity 를가지고있는 silica based filter 가사용되어큰 size 의 plasmid DNA 나많은양의 plasmid DNA 추출에매우효과적으로사용할수있습니다. Experimental Data Features and Benefits 높은 binding capacity를갖는 silica based DNA binding column 사용 enda+ strain을위한 Endonuclease A denaturation buffer 제공 (Denaturation Buffer, PD) DNA binding column 및 collection tube가들어있는 50 well tube rack은추출된 DNA 보관용 tube rack으로활용가능 Figure 1. Restriction enzyme (Xba I) digestion test. 200 ng of extracted plasmid DNA (pbluescript SK(+)) was digested with Xba I. Lane M: Molecular weight marker (λdna/hind III+EcoR I, Cat. no. D-1070, Bioneer) Lane 1: AccuPrep Plasmid Mini Extraction Kit Lane 2, 3: competitor s plasmid extraction kit Figure 2. Restriction enzyme digestion test. 200 ng of extracted plasmid DNA (pbluescript SK(+)) was digested with various restriction enzymes. Lane M: Molecular weight marker (λdna/hind III+EcoR I, Cat. no. D-1070, Bioneer) K: Kpn I, X: Xho I, SI: Sal I, H: Hind III, E: EcoR I, P: Pst I, Sm: Sma I, B: BamH I, S: Spe I, Xb: Xba I, N: Not I, Sc: Sac I 28

29 AccuPrep Plasmid Mini Extraction Kit Procedure Cat. no. Product Description K-3030 AccuPrep Plasmid Mini Extraction Kit, 200 rxn K AccuPrep Plasmid Mini Extraction Kit, 50 rxn KB-0101 RNase A powder, lyophilized (6 mg/tube) KA DNA Binding Column Tubes (50 ea X 4 box) 29

30 AccuPrep PCR Purification Kit Application Subcloning, Sequencing, Labeling, DNA concentration, etc. Experimental Data AccuPrep PCR Purification Kit 는 PCR 반응물을비롯한다양한효소반응물 ( 제한효소반응, A-tailing 반응, labeling 반응등 ) 로부터 5 분이내에각종불순물 (dimer, salts, dntps, enzymes, mineral oil, ethidium bromide, dye, detergent 등 ) 을완벽히제거하여고순도의 fragment DNA 를정제할수있는제품입니다. 본제품은 silica based DNA binding column 이사용된 spin column 방식의제품으로 binding buffer 와 washing buffer 및 elution buffer 로구성되어있습니다. 또한 elution volume 을 30 μl 까지줄일수있으므로 PCR product 농축에도효과적입니다. 정제된 fragment DNA 는 subcloning, sequencing, Southern blotting, Northern blotting 등에사용될수있습니다. Features and Benefits 100 bp부터 10 kb의 double strand DNA 및 single strand DNA 정제 평균 recovery는 70-90% 이상 전체실험소요시간이 5분미만으로매우빠르게실험가능 유기용매를사용하지않아매우안전하며, ethanol precipitation 과정이없어사용이편리함 recovery (%) B Q 200 bp 400 bp 1 kb 3 kb 6 kb Size of DNA fragment Figure 1. DNA size에따른 recovery 분석. 200 bp부터 6 kb까지의 fragment DNA를이용하여 recovery를분석한결과입니다. 평균 80% 이상의 recovery를보입니다. B: AccuPrep PCR Purification Kit Q: Competitor Q 1.0 kb M B B B Figure 2. Recovery analysis of the purified PCR product. 정제된 PCR product의정제효율을확인한결과입니다. Lane M: Molecular weight marker, 100 bp Plus ladder (D-1035, Bioneer) Lane 100: 100%, Lane 90: 90%, Lane 80: 80% Lane 70: 70%, Lane 60: 60%, Lane 50: 50% Lane B: purified PCR product 30

31 AccuPrep PCR Purification Kit Procedure Cat. no. Product Description K-3034 AccuPrep PCR Purification Kit, 200 rxn K AccuPrep PCR Purification Kit, 50 rxn KA DNA Binding Column Tubes (50 ea X 4 box) 31

32 AccuPrep Gel Purification Kit Specifications Subcloning, Sequencing, Labeling, DNA concentration, etc. Experimental Data AccuPrep Gel Purification Kit 는 Low melting agarose gel 뿐만아니라 TAE, TBE agarose gel 에서도원하는 DNA fragment 를 15 분이내에분리정제할수있도록고안된제품입니다. 또한, binding buffer 에 ph indicator 가함유되어있어최적의 binding 조건을잡을수있습니다. 본제품은 silica based DNA binding column 이사용된 spin column 방식의제품으로, 최적화된 binding buffer 와 washing buffer 및 elution buffer 가포함되어있어편리하게사용할수있습니다. 또한 elution volume 을 30 μl 까지줄일수있으므로 DNA 농축에도효과적입니다. Features and Benefits Low melting agarosge gel및 TAE/TBE agarose gel로부터 fragment DNA 추출 70 bp에서 10 kb 크기의 double strand DNA 및 single strand DNA 정제 전체실험소요시간이 15분미만으로매우빠르게실험가능 유기용매를사용하지않아안전하며, ethanol precipitation 과정이없어사용이편리함 recovery (%) B Q 70 bp 200bp 400bp 1 kb 3 kb 6 kb Size of DNA fragment Figure 1. DNA size에따른 recovery 70 bp부터 6 kb까지의 fragment DNA를이용하여 recovery를분석한결과입니다. B: AccuPrep Gel Purification Kit Q: Competitor Q 1.0 kb M B B B Figure 2. Recovery analysis of the purified fragment DNA. Agarose gel로부터 10.0 kb의 plasmid DNA를정제한후정제효율을확인한결과입니다. Lane M: Molecular weight marker, 100 bp Plus ladder (D-1035, Bioneer), Lane 100: 100%, Lane 90: 90%, Lane 80: 80% Lane 70: 70%, Lane 60: 60%, Lane 50: 50% Lane B: AccuPrep Gel Purification Kit 32

33 AccuPrep Gel Purification Kit Procedure Cat. no. Product Description K-3035 AccuPrep Gel Purification Kit, 200 rxn K AccuPrep Gel Purification Kit, 50 rxn KA DNA Binding Column Tubes (50 ea X 4 box) 33

DNA/RNA Amplification Overview AccuPower PreMix series 는세계적으로기술력을인정받은특허기술로보다경제적인가격과편리한방법으로실험할수있는제품입니다. Conventional PCR, Real-Time PCR 수행을위한 DNA ampli

DNA/RNA Amplification Overview AccuPower PreMix series 는세계적으로기술력을인정받은특허기술로보다경제적인가격과편리한방법으로실험할수있는제품입니다. Conventional PCR, Real-Time PCR 수행을위한 DNA ampli DNA Amplification RNA Amplification Real-Time PCR Customized PCR DNA/RNA Amplification Phone: 1588-9788 (ext.4->2) Email: accupower-support @bioneer.co.kr DNA/RNA Amplification Overview AccuPower PreMix

More information

Cloning

Cloning Takara 와함께하는 Cloning 2014-11-13 다카라코리아바이오메디칼 목차 Cloning 이란? Cloning Flow Chart Cloning DNA / RNA 추출 High Fidelity PCR 제한효소 /ligation/e.coli 형질전환 Clone 확인 이것만은꼭!!! 2 Cloning 이란? Clone 세포나개체의증식에의해서생긴유전적으로동일한세포군

More information

Selection chart of Bioneer s cdna synthesis products Categories Application Product cdna Synthesis Kits One step RT-PCR Kits One step RT-qPCR Kits RTa

Selection chart of Bioneer s cdna synthesis products Categories Application Product cdna Synthesis Kits One step RT-PCR Kits One step RT-qPCR Kits RTa Selection chart of Bioneer s cdna synthesis products Categories Application Product cdna Synthesis Kits One step RT-PCR Kits One step RT-qPCR Kits RTase 표준 cdna 합성 높은효율의 cdna 합성 복잡한 2 차구조 RNA 의 cdna 합성

More information

01 Buffers & Gel Stain Buffers 3 Gel Stain SilverStar Staining Kit 6

01 Buffers & Gel Stain Buffers 3 Gel Stain SilverStar Staining Kit 6 Buffers & Gel Stain Chemicals Buffers & Chemicals Phone: 1588-9788 (ext.4->2) Email: reagents-support@bioneer.co.kr 01 Buffers & Gel Stain Buffers 3 Gel Stain SilverStar Staining Kit 6 Buffers Overview

More information

Cloning=Clontech In-Fusion Cloning

Cloning=Clontech  In-Fusion Cloning Cloning 을위한모든것 2014-06-18 다카라코리아바이오메디칼 Contents Cloning Restriction Enzyme General Restriction Enzyme QuickCut Restriction Enzyme Cloning Restriction Enzyme/Ligation TA Cloning In-Fusion Cloning TaKaRa

More information

TOYOBO Reagent 만의독보적인기술 ReverTra Ace M-MLV RTase 의 RNase H domain 에 mutation 시키므로 RNase H activity 를낮추고,mRNA 의 degradation 을막아 cdna 합성효율을높임 KOD Polyme

TOYOBO Reagent 만의독보적인기술 ReverTra Ace M-MLV RTase 의 RNase H domain 에 mutation 시키므로 RNase H activity 를낮추고,mRNA 의 degradation 을막아 cdna 합성효율을높임 KOD Polyme PCR Enzyme 부터 qpcr 까지! PCR 실험은 TOYOBO 로해결하세요! 행사기간 : 7 월 15 일 ~ 9 월 13 일까지 TOYOBO Reagent PCR Enzyme High Quality PCR Enzyme cdna Synthesis Kit qpcr One-step RT Kit Immunoreaction Enhancer Solution 추가

More information

01 Automated Protein Expression and Purification: ExiProgen Selection Guide 3 Protein Synthesis Kit ExiProgen EC Protein Synthesis Kit 4 ExiProgen EC-

01 Automated Protein Expression and Purification: ExiProgen Selection Guide 3 Protein Synthesis Kit ExiProgen EC Protein Synthesis Kit 4 ExiProgen EC- Automated Protein Expression and Purification: ExiProgen TM Manual Protein Expression and Purification: AccuRapid TM & MagListo TM Preparation of Template DNA for Protein Expression Protein Service Protein

More information

01 Automated Protein Expression and Purification Using ExiProgen Selection Guide 267 ExiProgen EC Protein Synthesis Kit 268 ExiProgen EC-Maxi Protein

01 Automated Protein Expression and Purification Using ExiProgen Selection Guide 267 ExiProgen EC Protein Synthesis Kit 268 ExiProgen EC-Maxi Protein Automated Protein Expression and Purification Using ExiProgenTM Manual Protein Expression and Purification Preparation of Template DNA for Protein Expression Protein Service Protein Expression and Purification

More information

Ver.4 (KR) All about RT (cdna Synthesis) 8-11 Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: (Korea) (Internatio

Ver.4 (KR) All about RT (cdna Synthesis) 8-11 Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: (Korea) (Internatio 20170619 Ver.4 (KR) All about RT (cdna Synthesis) 8-11 Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: (Korea)1588-9788 (International) +82-42 - 930-8777 Email: sales@bioneer.com Selection

More information

슬라이드 1

슬라이드 1 TAKARA 1. PCR 2. qpcr 2014. 02. 13. 최유미 DNA? DNA 생물체 > 기관 > 세포 >DNA DNA (Deoxyribonucleic acid) 세포내에서생물의유젂정보를보관하는물질 구성 : Adenine, Thymine, Cytosine, Guanine 종특이적, 개체특이적 PCR (Polymerase Chain Reaction)

More information

항체 포털 서비스 Preparation Peptide 총 4주 소요 / 473,000 ~ 511,000 Free of charge 2~3 days 243,000 ~ 281,000 의뢰서 작성 Peptide epitope prediction 유전자 정보

항체 포털 서비스 Preparation Peptide 총 4주 소요 / 473,000 ~ 511,000 Free of charge 2~3 days 243,000 ~ 281,000 의뢰서 작성 Peptide epitope prediction 유전자 정보 Preparation Service AbFrontier Custom service 장점 실험 담당자와의 원활한 1:1 커뮤니케이션 (신속한 feedback을 통한 맞춤형 제작 서비스) 고객연구센터 - 개별 서비스의 진행 상황 및 결과 즉시 확인 http://center.abfrontier.com (e-mail & SMS 전송) 회원제 -연구자의 프로젝트 보안

More information

ITEM 1 PCR Enzyme 20% DNA Polymerase Enzyme Quick Selection Guide for Effective PCR DNA Polymerase General PCR / Colony PCR Long PCR (Max. 23 kb) Hot

ITEM 1 PCR Enzyme 20% DNA Polymerase Enzyme Quick Selection Guide for Effective PCR DNA Polymerase General PCR / Colony PCR Long PCR (Max. 23 kb) Hot 2017 Summer BIG SALE 2017.7.3 ~ 9.8 ITEM 1 P C R Enzyme cpcr Enzyme 20% High Quality Enzyme ITEM 2 cdna Synthesis cdna Synthesis Kit 최대 33 % off ITEM 3 q P C R Enzyme Two-Step / One-Step Kit NGS Library

More information

고품격 cdna 합성을위한 RT Kit M-MLV RTase 의 RNase H domain 에 mutation 시키므로 RNase H activity 를낮추고, mrna 의 degradation 을방지하여 cdna 합성효율을높임 d... qpcr RT Kit 의 cdn

고품격 cdna 합성을위한 RT Kit M-MLV RTase 의 RNase H domain 에 mutation 시키므로 RNase H activity 를낮추고, mrna 의 degradation 을방지하여 cdna 합성효율을높임 d... qpcr RT Kit 의 cdn TOYOBO 여름행사 21 행사기간ㅣ 2014 년 6 월 16 일 ~ 8 월 22 일까지 고품격 cdna 합성을위한 RT Kit 완벽한 qpcr 을위한최상의 Solution qpcr Master Mix ㅣ ReverTra Ace -a- kit ㅣ qpcr RT kit ㅣ qpcr RT Master Mix ㅣ qpcr RT Master Mix with gdna

More information

뉴스지14호-칼라

뉴스지14호-칼라 2 3 4 5 6 TaKaRa Taq TM / TaKaRa Ex Taq TM / TaKaRa LA Taq TM / Pyrobest TM DNA Polymerase/ TaKaRa Z -Taq TM TaKaRa Taq TM TaKaRa Ex Taq TM TaKaRa LA Taq TM TaKaRa Z-Taq TM Pyrobest TM DNA Polymerase 7

More information

mau A B C Qsepharose051229manual001:1_UV@01,SHFT Qsepharose051229manual001:1_Conc Qsepharose051229manual001:1_Fractions Qsepharose051229manual001:1_Inject Manual run 3:1_UV@01,SHFT Manual run 3:1_Fractions

More information

Ver.3(KR) All about Real-Time PCR 8-11, Munpyeongseoro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: (Korea) (International) +

Ver.3(KR) All about Real-Time PCR 8-11, Munpyeongseoro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: (Korea) (International) + 20170402 Ver.3(KR) All about Real-Time PCR 8-11, Munpyeongseoro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: (Korea) 1588-9788 (International) +82-42 - 930-8777 Email: sales@bioneer.com Real-Time

More information

2019 TOYOBO BIG SALE 기간 : 2019년 7월 1일 ~ 9월 11일까지 최대 33% SALE qpcr Enzyme PCR Enzyme 20% 2+1 cpcr Enzyme cdna Synthesis 2+1 cdna Synthesis kit High Qua

2019 TOYOBO BIG SALE 기간 : 2019년 7월 1일 ~ 9월 11일까지 최대 33% SALE qpcr Enzyme PCR Enzyme 20% 2+1 cpcr Enzyme cdna Synthesis 2+1 cdna Synthesis kit High Qua 2019 TOYOBO BIG SALE 기간 : 2019년 7월 1일 ~ 9월 11일까지 최대 33% SALE qpcr Enzyme PCR Enzyme cpcr Enzyme cdna Synthesis cdna Synthesis kit High Quality Enzyme Immuno Enhancer Cloning One-Step kit Can Get Signal

More information

Automated high multiplex qPCR platform for simultaneous detection and quantification of multiple nucleic acid targets

Automated high multiplex qPCR platform for simultaneous detection and quantification of multiple nucleic acid targets PCR FlashGel System 2013. 04. 24 PCR 2013. 04. 24 순서 1. PCR 이란 2. PCR 실험의기본조건 3. PCR 효소선택가이드 4. Hot Start PCR의원리 5. PCR 실험시 Troubleshooting 6. PCR 장비 _TP350 소개 PCR(Polymerase Chain Reaction) PCR 이란? -

More information

EZ-Cloning kit

EZ-Cloning kit EZ TM 5 /3 RACE PCR Kit Instruction Manual Korean Ver. Kit for 10 reactions Cat.# EZ025 EZ TM 5 /3 RACE PCR Kit Table of Contents I. 제품설명... 3 II. 원리... 4 III. 제품구성... 7 IV. 염기서열정보... 8 V. 주의사항... 8 VI.

More information

DNA Polymerase Enzyme Quick Selection Guide for Effective PCR DNA Polymerase High Quality Polymerase General PCR / Colony PCR High Fidelity PCR (80-fo

DNA Polymerase Enzyme Quick Selection Guide for Effective PCR DNA Polymerase High Quality Polymerase General PCR / Colony PCR High Fidelity PCR (80-fo 2018 TOYOBO BIG SALE 기간 : 2018년 7월 16일 ~ 9월 21일까지 TOYOBO 전제품 최대 33% SALE PCR Enzyme cpcr Enzyme cdna Synthesis cdna Synthesis Kit qpcr Enzyme One-Step / Two-Step Kit Immuno Enhancer High Quality Enzyme

More information

SMARTer 시리즈

SMARTer 시리즈 SMARTer 시리즈 SMARTer Solutions 2014.12.17 다카라코리아바이오메디칼 SMART 하게실험하고 SMARTer 한결과를얻는방법 Clontech SMARTer 시리즈 Contents 1. Introduction 1. 역전사반응이란? 2. 기존역전사반응의한계 2. SMARTer 시리즈 1. SMARTer 란? 2. SMARTer 시리즈와적용

More information

Surpass the limit of Polymerase Chain Reaction (PCR) PCR Enzyme Guide High Fidelity PCR 효소 범용적인 PCR 효소 특수목적을위한 PCR 효소 - Epigenetics - Multiplex PCR -

Surpass the limit of Polymerase Chain Reaction (PCR) PCR Enzyme Guide High Fidelity PCR 효소 범용적인 PCR 효소 특수목적을위한 PCR 효소 - Epigenetics - Multiplex PCR - Surpass the limit of Polymerase Chain Reaction (PCR) PCR Enzyme Guide High Fidelity PCR 효소 범용적인 PCR 효소 특수목적을위한 PCR 효소 - Epigenetics - Multiplex PCR - Direct PCR High Fidelity PCR 효소 - NGS targeted sequencing,

More information

PCR DNA Polymerase 선택가이드 일반적인 PCR 실험 Blend Taq / Blend Taq -Plus- Repairing 일반 DNA polymerase와 proofreading 기능의효소가섞인제품 Taq polymerase 보다 3~4 배낮은 error

PCR DNA Polymerase 선택가이드 일반적인 PCR 실험 Blend Taq / Blend Taq -Plus- Repairing 일반 DNA polymerase와 proofreading 기능의효소가섞인제품 Taq polymerase 보다 3~4 배낮은 error TOYOBO HOT SUMMER BIG SALE 행사기간ㅣ 2015 년 6 월 22 일 ~ 8 월 28 일까지 2+1 PCR DNA Polymerase cdna Synthesis Kit ㅣ KOD Series ㅣ Blend Taq Series ㅣ Quick Taq HS DyeMix ㅣ ReverTra Ace Series 플러스혜택 Set 구매시, 최대 45%

More information

Print

Print 2018-09-11 Ver.4 (KR) 8-11, Munpyeongseo-ro, Daedeok-gu, Daejeon, 34302, Republic of Korea Tel: (Korea) 1588-9788 (International) +82-42-930-8777 Email: sales@bioneer.com Real-Time PCR Reagents Selection

More information

ReverTra Ace cdna Synthesis Kit d ReverTra Ace M-MLV RTase의 RNase H domain 부위에유전자조작을시켜, Rnase H activity를낮추어 mrna의 degradation을방지하여 cdna 합성효율을높임 Long

ReverTra Ace cdna Synthesis Kit d ReverTra Ace M-MLV RTase의 RNase H domain 부위에유전자조작을시켜, Rnase H activity를낮추어 mrna의 degradation을방지하여 cdna 합성효율을높임 Long 2016 TOYOBO SUMMER SALE 2016.7.4 ~ 9.9 cdna Synthesis Kit qpcr Master Mix KOD Polymerase Enzyme DNA Polymerase Enzyme Immuno Enhancer ReverTra Ace cdna Synthesis Kit d ReverTra Ace M-MLV RTase의 RNase H

More information

Product Description Apoptosis 혹은 necrosis등에의하여죽거나손상된세포에서방출되는 Lactate dehydrogenase(ldh) 의양을고감도로측정함으로써 cytotoxicity/cytolysis를간단하게측정할수있는 kit 입니다. Cytot

Product Description Apoptosis 혹은 necrosis등에의하여죽거나손상된세포에서방출되는 Lactate dehydrogenase(ldh) 의양을고감도로측정함으로써 cytotoxicity/cytolysis를간단하게측정할수있는 kit 입니다. Cytot EZ-LDH Cell Cytotoxicity Assay Kit Cat. No. DG-LDH500 DG-LDH1000 FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. 1 ( 주 ) 대일랩서비스부설두젠생명공학연구소 Product Description Apoptosis 혹은 necrosis등에의하여죽거나손상된세포에서방출되는

More information

Chapter 4. Bacteria (the first microbes)

Chapter 4. Bacteria (the first microbes) - DNA 정제후클로닝을위한효소적절단과연결필요 -> restriction enzyme, ligase 가중요 1. DNA 조작효소범위 - 촉매반응에따라 5 분류 1. Nuclease : 핵산분자의절단, 단편화 2. Ligase : 핵산분자의연결 3. Polymerase : DNA 사슬합성 4. Modifying enzyme : 화학기능기의첨가, 제거 5. Topoisomerase

More information

슬라이드 1

슬라이드 1 EVENT 퀴아젠인기상품연말특별가전 200203 Top Taq DNA Polymerase (250U) 90,000 72,000 200205 Top Taq DNA Polymerase (1000U) 331,000 281,350 200403 Top Taq Master Mix Kit(250U) 104,000 83,200 201203 Taq DNA Polymerase

More information

PowerPoint 프레젠테이션

PowerPoint 프레젠테이션 Glutathione Excellose handbook - Purification of GST fusion proteins - Looking for more detail Purification protocol? - Glutathione Excellose Spin Kit - Glutathione MiniExcellose - Glutathione Excellose

More information

System Biology Core

System Biology Core System Biology Core 연세의생명연구원연구지원부 _ 연세유전체센터 The RNAi Consortium (TRC) shrna 안내 Ⅰ. MISSION shrna 의설계 MIT 와 Harvard 의 Broad Institute 의 The RNAi Consortium (TRC) 에서설계하고개발한 MISSION shrna clones 는 21 base

More information

Spring SALE 개나리 꽃이 피었습니다! 당신의 얼굴에도 웃음 꽃이 피었습니다! Seakem LE Agarose (Cat. No ) One하면 덤으로 한 개 더! 1+1 기간 : 2011년 3월 2일 ~ 4월 15일 ~ 30 % Sa le 고객지원센터

Spring SALE 개나리 꽃이 피었습니다! 당신의 얼굴에도 웃음 꽃이 피었습니다! Seakem LE Agarose (Cat. No ) One하면 덤으로 한 개 더! 1+1 기간 : 2011년 3월 2일 ~ 4월 15일 ~ 30 % Sa le 고객지원센터 Spring SALE 개나리 꽃이 피었습니다! 당신의 얼굴에도 웃음 꽃이 피었습니다! Seakem LE Agarose (Cat. No. 50004) One하면 덤으로 한 개 더! 1+1 기간 : 2011년 3월 2일 ~ 4월 15일 ~ 30 % Sa le 고객지원센터 (학술/제품문의) 02-2081 - 2510 www.takara.co.kr support@takara.co.kr

More information

미생물분류는형태적특징, 생리 생화학적성질과상태, 화학분류학적성질과상태등을이용하여구분하는것이일반적이지만, 이와같은방법을이용하면많은시간을필요로한다. 또한분류가힘든경우나, 정확하지못한결과를얻는경우도있다. 최근미생물분류에도분자생물학적인방법을이용하여, 미생물이가지고있는 DNA를

미생물분류는형태적특징, 생리 생화학적성질과상태, 화학분류학적성질과상태등을이용하여구분하는것이일반적이지만, 이와같은방법을이용하면많은시간을필요로한다. 또한분류가힘든경우나, 정확하지못한결과를얻는경우도있다. 최근미생물분류에도분자생물학적인방법을이용하여, 미생물이가지고있는 DNA를 Code RR180A - 연구용 - Bacterial 16S rdna PCR Kit 사용설명서 v201011da 미생물분류는형태적특징, 생리 생화학적성질과상태, 화학분류학적성질과상태등을이용하여구분하는것이일반적이지만, 이와같은방법을이용하면많은시간을필요로한다. 또한분류가힘든경우나, 정확하지못한결과를얻는경우도있다. 최근미생물분류에도분자생물학적인방법을이용하여, 미생물이가지고있는

More information

3월 온라인 교육

3월 온라인 교육 2013 년 2 분기대리점집체교육 - Protein - Takara Korea Biomedical Protein Workflow Chapter 1: 샘플준비 Chapter 2: Electrophoresis -Precast gel (Lonza/NuSep) -ProSieve EX running buffer -Protein marker Chapter 3: Staining

More information

01 DNA Polymerase Selection Guide 293 Top DNA Polymerase 294 Taq DNA Polymerase 296 Pfu DNA Polymerase 298 ProFi Taq DNA Polymerase 300 HotStart DNA P

01 DNA Polymerase Selection Guide 293 Top DNA Polymerase 294 Taq DNA Polymerase 296 Pfu DNA Polymerase 298 ProFi Taq DNA Polymerase 300 HotStart DNA P DNA Polymerase Reverse Transcriptase DNA Ligase Restriction Enzymes Enzymes Phone: 1588-9788 (ext.4->2) Email: reagents-support @bioneer.co.kr 01 DNA Polymerase Selection Guide 293 Top DNA Polymerase 294

More information

Microsoft Word - Genolution RNAi Manual.doc

Microsoft Word - Genolution RNAi Manual.doc 1 Ⅵ. G-Fectin (transfection reagent) RNAi transfection 전용 reagent. Transfection 전 과정 10분 이내 완료. 24시간 후 gene silencing 확인 가능. 우수한 transfection 효율 낮은 toxicity. sirna와 shrna, mirna에 모두 적용 가능. 가격 : 150,000

More information

Glutathione Excellose handbook - Purification of GST fusion proteins - Looking for more detail Purification protocol? - Glutathione Excellose Spin Kit

Glutathione Excellose handbook - Purification of GST fusion proteins - Looking for more detail Purification protocol? - Glutathione Excellose Spin Kit Glutathione Excellose handbook - Purification of GST fusion proteins - Looking for more detail Purification protocol? - Glutathione Excellose Spin Kit - Glutathione MiniExcellose - Glutathione Excellose

More information

Slide 1

Slide 1 PrimeScript 1st strand cdna Synthesis Kit 1 Central Dogma replication transcription 0 processing translation 오늘의내용은? 조직추출 RNAiso Plus 5 mrna 우리가관심있는 mrna가차지하는비율이극히일부이기때문에관심있는 mrna를증폭해서그것을눈으로확인할정도로만들어주는것이다.

More information

cdna의신장반응이저해된다. 이와같이 AMV 유래 RTase 와 MoMLV 유래 RTase 는모두일장일단을가지나필자는 MoMLV 유래 RTase 를선호한다. 또두효소는최적 ph, 최적염농도등에서도차이가있으므로주의해야한다. 최근 Myers 등은 RTase 활성과 PCR

cdna의신장반응이저해된다. 이와같이 AMV 유래 RTase 와 MoMLV 유래 RTase 는모두일장일단을가지나필자는 MoMLV 유래 RTase 를선호한다. 또두효소는최적 ph, 최적염농도등에서도차이가있으므로주의해야한다. 최근 Myers 등은 RTase 활성과 PCR RT-PCR 법 II. 역전사효소반응 ( 주 ) 다인바이오연구소 현재분자생물학분야에서 RNA 수준의 gene expression ( 유전자발현 ) 과 cdna (complementary DNA) cloning에널리이용되고있는 RT-PCR (Reverse transcription ploymerase chain reaction) 은생명정보가 DNA에서 RNA로전달된다는분자생물학분야의

More information

Technical/Specs Sequencing 서비스안내 Standard Sequencing Plasmid/PCR product 를 primer 를이용하여고객이원하는 region 을분석하는서비스이며, 대부분의 normal 샘플에대해 최적화되어있습니다. Full Len

Technical/Specs Sequencing 서비스안내 Standard Sequencing Plasmid/PCR product 를 primer 를이용하여고객이원하는 region 을분석하는서비스이며, 대부분의 normal 샘플에대해 최적화되어있습니다. Full Len Technical/Specs Sequencing 서비스안내 Standard Sequencing Plasmid/PCR product 를 primer 를이용하여고객이원하는 region 을분석하는서비스이며, 대부분의 normal 샘플에대해 최적화되어있습니다. Full Length Sequencing (Primer walking) 길이가긴 insert 를포함한 plasmid

More information

실험 Set Up Guide 실험주제 Real Time PCR 실험원리 Quantitative Real-Time PCR (qrt-pcr) 은 1992년에도입되어생명공학에응용되기시작하였이기술은잘알려져있는 PCR기법을개량한것이다. PCR은핵산의효소증폭을이용하며, 극히적은양

실험 Set Up Guide 실험주제 Real Time PCR 실험원리 Quantitative Real-Time PCR (qrt-pcr) 은 1992년에도입되어생명공학에응용되기시작하였이기술은잘알려져있는 PCR기법을개량한것이다. PCR은핵산의효소증폭을이용하며, 극히적은양 실험 Set Up Guide 실험주제 Real Time PCR 실험원리 Quantitative Real-Time PCR (qrt-pcr) 은 1992년에도입되어생명공학에응용되기시작하였이기술은잘알려져있는 PCR기법을개량한것이다. PCR은핵산의효소증폭을이용하며, 극히적은양의시료를대량으로증폭할수있다는장점과그과정이간단하여 Cloning, Sequencing 등의기본기술로응용되었다.

More information

슬라이드 1

슬라이드 1 Real Time PCR 목차 1. PCR 및 Real Rime PCR 원리 2. Real Time PCR 검출방법 3. Real Time PCR 정량방법 4. Takara Real Time PCR 시약 5. 금일실험내용 Polymerase Chain Reaction Taq DNA Polymerase - Thermostable DNA polymease from

More information

2018 ASF Standard Operation Procedure 아프리카돼지열병진단개요 : - African swine fever, Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. Chapter2.

2018 ASF Standard Operation Procedure 아프리카돼지열병진단개요 : - African swine fever, Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. Chapter2. 2018 ASF Standard Operation Procedure 아프리카돼지열병진단개요 : - African swine fever, Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. Chapter2.8.1. OIE 2012 ver. - EU ASF reference lab CISA-INIA(Spain)

More information

- 3 - 1 10. 10. 12 1. 12 2. 12. 13 2 14 2.1 14 2.2 17 2.3 18 2.4 19 2.5 21 (1) 21 (2) DNA 23 (3) 24 (4) 16S rrna 25 (5) (Polymerase chain reaction, PCR) 26 (6) PCR Primer 27 2.6 28. / 28-4 - (1) Bioaerosol

More information

DBPIA-NURIMEDIA

DBPIA-NURIMEDIA Short ommunication Journal of piculture 33(3) : 221~226 (218) DOI: 1.17519/apiculture.218.9.33.3.221 Detection of Sugar ane (Saccharum officinarum)-specific Gene from Sugar and Sugar-honey younghee Kim,

More information

(Establishment and Management of Proteomics Core Facility)

(Establishment and Management of Proteomics Core Facility) (Establishment and Management of Proteomics Core Facility) 1 sample running on SDS-PAGE Gel (10well) 163570 10 16357 1D size marker 225500 100 2255 Buffer(20X, 500ml) 119900 20 5995 Staining

More information

BIONEER CORPORATION Preface 본책자는포스트게놈시대의생명공학연구실의필수적인연구장비인 ExiProgen 에관한설명서입니다. ExiProgen 은무세포 transcription, translation 반응과 magnetic affinity purification 을이용하여 DNA 로부터다양한단백질들을전자동으로합성하고고순도로정제할수있어생명공학의획기적인발전을가져올수있는신개념의장비입니다.

More information

PowerPoint Presentation

PowerPoint Presentation Interchapeter B : 핵산생물공학기술 (part A) Recombinant DNA techniques - 전기영동 - 제한효소 - 클로닝 - 대장균을이용한단백질대량생산 Agarose mesh DNA 분리기술 : Agarose Gel Electrophoresis ( 수평형 ) _ + DNA is negatively charged from the phosphate

More information

DBPIA-NURIMEDIA

DBPIA-NURIMEDIA Mass-production of Four Specific Proteins Originated from Deformed Wing Virus, Honeybee-viral Pathogen Joo-seong Lee, Giang Thi Huong Luong and Byoung-Su Yoon* Department of Life Science, College of Natural

More information

(....).hwp

(....).hwp 연구기관 한국식품연구원 농림부 연구기관 한국식품연구원 농림부 Analytical Semi-preparative Solution A 1) Solution B 2) Mixing Spreading Coating medium Corona treated OPP 3) film Casting Drying

More information

05052유식안101.hwp

05052유식안101.hwp 제 출 문 식품의약품안전청장 귀 하 이 보고서를 유전자재조합식품 모니터링(부산광역시보건환경연구원/정구영) 과제의 연구 결과보고서로 제출합니다. 2005. 11. 30 주관연구기관명 : 부산광역시보건환경연구원 주관연구책임자 : 정구영 목 차 Ⅰ. 연구개발결과 요약문----------------------------------- 1 (한글)------------------------------------------

More information

PowerPoint 프레젠테이션

PowerPoint 프레젠테이션 IDA Excellose handbook - Purification of polyhistidine tagged proteins - Looking for more detail Purification protocol? - Chelating Excellose Spin Kit - IDA MiniExcellose - IDA Excellose www.takara.co.kr

More information

Sequencing Service DNA sequencing Phone: (ext.4->4)

Sequencing Service DNA sequencing Phone: (ext.4->4) DNA sequencing Phone: 1588-9788 (ext.4->4) Email: sequencing@bioneer.co.kr 01 3 Overview 바이오니아는국내최초로 를시작하였으며, 매년개선된 high-throughput sequencing service 를제공하고있습니다. Plasmid DNA, PCR product 등을비롯하여다양한종류의 template

More information

ExiProgen His-tagged Protein Purification Kit 사용설명서 Version No.: 1.0 ( ) Please read all the information in booklet before using the kit 바이오니아 대

ExiProgen His-tagged Protein Purification Kit 사용설명서 Version No.: 1.0 ( ) Please read all the information in booklet before using the kit 바이오니아 대 사용설명서 Version No.: 1.0 (2013-10) Please read all the information in booklet before using the kit 바이오니아 대전광역시대덕구문평서로 8-11 Tel: +82-1588-9788 Fax: +82-42-930-8600 Email: order@bioneer.co.kr Safety warning

More information

DBPIA-NURIMEDIA

DBPIA-NURIMEDIA Original Article Journal of Apiculture 31(2) : 121~131 (2016) The Most Rapid Detection Method against Korean Sacbrood Virus using Ultra-Rapid Reverse-Transcription Real-Time PCR (URRTRT-PCR) Sang-Hyun

More information

Chapter 26

Chapter 26 11 주 RNA 합성 11.1 DNA-dependent synthesis of RNA: Bacteria에서의 transcription l RNA polymerase (5 subunits로구성 : 2α, β, β, σ) l 효소에의한 RNA 합성의특징 - Complementary sequence to template DNA - RNA chain의합성방향 : 5

More information

- 1 -

- 1 - - 1 - - 2 - - 3 - - 4 - - 5 - - 6 - ι κ λ β β β β β - 7 - - 8 - - 9 - - 1 - - 11 - 마. - 12 - - 13 - - 14 - - 15 - - 16 - - 17 - - 18 - - 19 - - 2 - - 21 - - 22 - - 23 - - 24 - ι κ λ β β - 25 - - 26 - -

More information

한약재품질표준화연구사업단 단삼 ( 丹參 ) Salviae Miltiorrhizae Radix 생약연구과

한약재품질표준화연구사업단 단삼 ( 丹參 ) Salviae Miltiorrhizae Radix 생약연구과 한약재품질표준화연구사업단 단삼 ( 丹參 ) Salviae Miltiorrhizae Radix 생약연구과 - 1 - KP 11 CP 2015 Salvia miltiorrhizae Radix Salviae Miltiorrhizae Radix et Rhizoma Salvia miltiorrhiza Bunge Salvia miltiorrhiza Bunge salvianolic

More information

-, BSF BSF. - BSF BSF ( ),,. BSF -,,,. - BSF, BSF -, rrna, BSF.

-, BSF BSF. - BSF BSF ( ),,. BSF -,,,. - BSF, BSF -, rrna, BSF. 1. 2010 6 1, 2 4 Ⅰ,Ⅱ 2013,, -,. - Human Cell.. -,., Biosand Filter(BSF) - BSF Main Filtering (Biofilm) BSF, BSF ( ),. -, BSF BSF. - BSF BSF ( ),,. BSF -,,,. - BSF,. -. -. -. - 2. BSF -, rrna, BSF. 2. -

More information

MHC T cell Receptor Antigen Antigen-presenting cell Antigen-reactive T cell CD28 B7 Activation (proliferation Cytokine production Cytotoxicity) Antigen-presenting cell Antigen-reactive T cell Dormant State

More information

00.1

00.1 HOSPA Chipboard screws with countersunk head Material: Drive: Cross recess PZ galvanized yellow chromatized nickel plated burnished Partly threaded, galvanized or yellow chromatized dk k L d m Head Ø dk

More information

l l l l l l l l l Lee, Geon Kook None This project was designed to establish the Tumor Bank of National Cancer Center in 2000. From the first tumor sample in 2000, the total of tumor and tumor-related

More information

Nuclease (핵산 분해 효소)

Nuclease (핵산 분해 효소) 7. Nuclease ( 핵산분해효소 ) Nuclease Nuclease 란? DNA 를중합하는 DNA polymerase 와는달리핵산을분해하는효소 Nuclease 의중요성 생체내에서는항상핵산을분해하는일이일어나고있으므로생명현상을공부한다는점에서중요 실제의분자생물학실험에서 polymerase 보다더자주쓰이고있기때문에중요 Nuclease Nuclease 를실험에이용할때염두해두어야할내용

More information

슬라이드 1

슬라이드 1 LC-MS 단백질분석을위한 SDS-PAGE staining protocol 및주의할점 Proteomics Core Facility 2017.12.28 Contents LC-MS 분석의뢰용 protein SDS-PAGE staining 시주의할점 Coomassie Blue R-250 을이용한 gel staining (general protocol) Coomassie

More information

Microsoft PowerPoint - 12-전기영동.pptx

Microsoft PowerPoint - 12-전기영동.pptx 전기영동 실험목적 기본적인분자실험장비인피펫, 저울, 원심분리 기, vortex mixer 사용법숙지 전기영동과정을통해서 DNA 크기비교 여러가지피펫종류들 Basic type P2 white tip P10 white tip P20 yellow tip P100 yellow tip P200 yellow tip P1000 blue tip Multi-channel pipette

More information

DC Link Application DC Link capacitor can be universally used for the assembly of low inductance DC buffer circuits and DC filtering, smoothing. They

DC Link Application DC Link capacitor can be universally used for the assembly of low inductance DC buffer circuits and DC filtering, smoothing. They DC Link Capacitor DC Link Application DC Link capacitor can be universally used for the assembly of low inductance DC buffer circuits and DC filtering, smoothing. They are Metallized polypropylene (SH-type)

More information

한약재품질표준화연구사업단 금은화 ( 金銀花 ) Lonicerae Flos 생약연구과

한약재품질표준화연구사업단 금은화 ( 金銀花 ) Lonicerae Flos 생약연구과 한약재품질표준화연구사업단 금은화 ( 金銀花 ) Lonicerae Flos 생약연구과 - 2 - KP 11 Lonicerae Flos Lonicera japonica Thunberg - CP 2015 JP 16 Lonicerae Japonicae Flos Lonicerae Folium Cum Caulis Lonicera japonica Thunberg - Lonicera

More information

Preliminary spec(K93,K62_Chip_081118).xls

Preliminary spec(K93,K62_Chip_081118).xls 2.4GHz Antenna K93- Series KMA93A2450X-M01 Antenna mulilayer Preliminary Spec. Features LTCC Based designs Monolithic SMD with small, low-profile and light-weight type Wide bandwidth Size : 9 x 3 x 1.0mm

More information

DNA Sequencing Service Sequencing Reagents DNA sequencing Phone: (ext.4->4)

DNA Sequencing Service Sequencing Reagents DNA sequencing Phone: (ext.4->4) Sequencing Reagents DNA sequencing Phone: 1588-9788 (ext.4->4) Email: sequencing@bioneer.co.kr 01 217 Overview 바이오니아는국내최초로 Sequencing Service 를시작하였으며, 매년개선된 high-throughput sequencing service 를제공하고있습니다.

More information

증정 Bio-Rad 가제안하는 FAST Western Blot! FAST Western Blot 기존 acrylamide/bis solution FastCast TM TGX acrylamide solution Gel 준비 (Gel solution 준비 - casting

증정 Bio-Rad 가제안하는 FAST Western Blot! FAST Western Blot 기존 acrylamide/bis solution FastCast TM TGX acrylamide solution Gel 준비 (Gel solution 준비 - casting Bio-Rad 연말할인행사 기간 : 2015 년 10 월 19 일 ~ 12 월 18 일까지 FAST Western Blot Best Western Blot 을위한시약 & 소모품 Real-Time PCR Reagents 행사제품 400 만원이상구매시, Integra PIPETBOY ACU 증정 ( 색상랜덤 ) 증정다양한 application 에따라흡입 / 분주속도조절가능

More information

뉴스레터6호F?2??訝

뉴스레터6호F?2??訝 February 2009 No.06 Roche Diagnostics Korea Co., Ltd. Focus Tech EDITORIAL February 2009 No.06 Contents Editorial 03 Focus 04 Product 10 Talk 12 Tech 14 Activity 19 Style 22 February 2009 No.06 02 03 FOCUS

More information

슬라이드 1

슬라이드 1 DNA technology (DNA 조작기술 ) 유전자 cloning Cloning & Plasmid Joshua Ledergerg & Edward Tatum, 1946 두종류의다른유전자를가진대장균사이에유전자의재조합이일어나새로운대장균이만들어지는것을발견했다. 이원리를바탕으로 1970 년대에 DNA 재조합기술이발전하게되었다. 원핵생물에서 DNA 의이동방법 플라스미드를이용한

More information

(Table of Contents) 2 (Specifications) 3 ~ 10 (Introduction) 11 (Storage Bins) 11 (Legs) 11 (Important Operating Requirements) 11 (Location Selection)

(Table of Contents) 2 (Specifications) 3 ~ 10 (Introduction) 11 (Storage Bins) 11 (Legs) 11 (Important Operating Requirements) 11 (Location Selection) SERVICE MANUAL (Table of Contents) 2 (Specifications) 3 ~ 10 (Introduction) 11 (Storage Bins) 11 (Legs) 11 (Important Operating Requirements) 11 (Location Selection) 12 (Storage Bins) 12 (Ice Machine)

More information

Conventional PCR: AllInOneCycler Real-Time PCR: Exicycler 96 Protein Synthesis and Purification: ExiProgen DNA/RNA Preparation: ExiPrep 16 Plus & ExiC

Conventional PCR: AllInOneCycler Real-Time PCR: Exicycler 96 Protein Synthesis and Purification: ExiProgen DNA/RNA Preparation: ExiPrep 16 Plus & ExiC Conventional PCR: AllInOneCycler Real-Time PCR: Exicycler 96 Protein Synthesis and Purification: ExiProgen DNA/RNA Preparation: ExiPrep 16 Plus & ExiCracker Electrophoresis: Agaro-Power Microbial Culture:

More information

SMARTer Sequencing Kits for Next Generation Sequencing

SMARTer Sequencing Kits for Next Generation Sequencing Simple, Fast, Powerful SMARTer Solution for NGS 다카라코리아바이오메디칼 1 페이지 표지분석 NGS Library 제작시의어려움을, SMARTer 기술로극복! FFPE Sample 과같은 Low input, degraded RNA 로부터 Sequencing 을가능하게! Single Cell sample 로부터 Library

More information

PowerPoint 프레젠테이션

PowerPoint 프레젠테이션 Product News Letter 4 호 User s Guide for Ligation Independent Cloning Inspect into "Overlap Cloner" Cloning 의일반적인방법 미래기술 Recombineering "Overlap Cloner" 의원리와응용분야 자주하는질문 Trouble Shooting 은단백질전문기술기업입니다기술혁신형중소기업입니다고객상담전화

More information

DBPIA-NURIMEDIA

DBPIA-NURIMEDIA Original Article Journal of Apiculture 31(1) : 41~50 (2016) Rapid Detection of Black Queen Cell Virus from Honeybee using Reverse Transcription Real-Time Recombinase Polymerase Amplification (RT/RT RPA)

More information

프로메가 2018 여름이벤트 BIG SALE 행사기간 2018년 8월 06일 ~ 9월 14일 *본 행사지에 표기된 금액은 부가세 별도 금액입니다. *단가 계약 및 입찰은 행사에서 제외됩니다.

프로메가 2018 여름이벤트 BIG SALE 행사기간 2018년 8월 06일 ~ 9월 14일 *본 행사지에 표기된 금액은 부가세 별도 금액입니다. *단가 계약 및 입찰은 행사에서 제외됩니다. 프로메가 2018 여름이벤트 BIG SALE 행사기간 2018년 8월 06일 ~ 9월 14일 *본 행사지에 표기된 금액은 부가세 별도 금액입니다. *단가 계약 및 입찰은 행사에서 제외됩니다. EtBr 을아직사용하신다면? 일회용 Gel extractor 를사용해보세요! 내몸은소중하니깐 ~~~ 15ul minimum elution 이가능합니다!! 프로메가 Steady

More information

α α α α α

α α α α α α α α α α α α α 太陰調胃湯加減方 dbdb 마우스 肝에 대한 아디포사이토카인 및 발현에 미치는 영향 SREBPs 와 섞어서 하고 마커 또한 하여 전기 영동을 하였다 전기영동을 한 후에 에 를 쪼여서 각 를 확인하였다 이 를 프로그램을 이용해 수치화하 여 분석하였다 肝 조직 동결 절편 분리한 조직은 로 시간 동안 고정 시킨 후 에 세척한 후 물기를

More information

Pierce-Plus-Alpha.pdf

Pierce-Plus-Alpha.pdf Save 40% on antibodies Primary antibody 전제품 40% 할인가로만나보세요 Count on Invitrogen 42,000 antibodies highly validated primary and secondary antibodies in our portfolio and growing 4,500 primary conjugated antibodies

More information

(specifications) 3 ~ 10 (introduction) 11 (storage bin) 11 (legs) 11 (important operating requirements) 11 (location selection) 12 (storage bin) 12 (i

(specifications) 3 ~ 10 (introduction) 11 (storage bin) 11 (legs) 11 (important operating requirements) 11 (location selection) 12 (storage bin) 12 (i SERVICE MANUAL N200M / N300M / N500M ( : R22) e-mail : jhyun00@koreacom homepage : http://wwwicematiccokr (specifications) 3 ~ 10 (introduction) 11 (storage bin) 11 (legs) 11 (important operating requirements)

More information

F. Sequencing FSequencing 01. Sequencing Service DNA sequencing Phone: (ext.4 4)

F. Sequencing FSequencing 01. Sequencing Service DNA sequencing Phone: (ext.4 4) FSequencing 01. DNA sequencing Phone: 1588-9788 (ext.4 4) E-mail: sequencing@bioneer.co.kr 01. 242 FAQs 247 개요 상세 설명 바이오니아는 국내 최초로 를 시작하였으며, 매년 개선된 high-throughput sequencing service를 제공하고 있습니다. Plasmid

More information

Statistical Data of Dementia.

Statistical Data of Dementia. Screening of Prolyl Endopeptidase Inhibitors from Medicinal Plants. Lee Si-young, Lee Jung-han, Paik Young-Sook College of Environment and Applied Chemistry, Kyung Hee University. Statistical Data of Dementia.

More information

(Exposure) Exposure (Exposure Assesment) EMF Unknown to mechanism Health Effect (Effect) Unknown to mechanism Behavior pattern (Micro- Environment) Re

(Exposure) Exposure (Exposure Assesment) EMF Unknown to mechanism Health Effect (Effect) Unknown to mechanism Behavior pattern (Micro- Environment) Re EMF Health Effect 2003 10 20 21-29 2-10 - - ( ) area spot measurement - - 1 (Exposure) Exposure (Exposure Assesment) EMF Unknown to mechanism Health Effect (Effect) Unknown to mechanism Behavior pattern

More information

01 sirna AccuTarget TM sirnas 35 Custom sirna 36 Genome-wide sirna 38 AccuTarget Genome-wide Predesigned sirna Library 39 AccuTarget Premade sirna Set

01 sirna AccuTarget TM sirnas 35 Custom sirna 36 Genome-wide sirna 38 AccuTarget Genome-wide Predesigned sirna Library 39 AccuTarget Premade sirna Set Custom sirna Genome-wide sirna sirna Oligonucleotide FAQs AccuTarget mirna mimics & inhibitors AccuTarget mirna Controls sirna & mirna Phone: 1588-9788 (ext.4->1) Email: sirna@bioneer.co.kr Email: sirna-support@bioneer.co.kr

More information

<313630313032C6AFC1FD28B1C7C7F5C1DF292E687770>

<313630313032C6AFC1FD28B1C7C7F5C1DF292E687770> 양성자가속기연구센터 양성자가속기 개발 및 운영현황 DOI: 10.3938/PhiT.25.001 권혁중 김한성 Development and Operational Status of the Proton Linear Accelerator at the KOMAC Hyeok-Jung KWON and Han-Sung KIM A 100-MeV proton linear accelerator

More information

WIDIN - Toolholding Catalogue.pdf

WIDIN - Toolholding Catalogue.pdf T CH CHUC UCK K 60 ER Strong Torque Power ER Chuck have strong torque power. Slim designed ER Nut were minimized an interruption to workpiece. If using Carbide Drill and coated drill, it can be improve

More information

Title: Biacore getting started kit 를이용한 KD 값도출방법 목차 1. 실험목적 2. 실험개요와필요한시약및소모품 2.1 실험개요 2.2 필요한시약및소모품 3. 실험방법과결과 3.1 ph-scouting for pre-concentration

Title: Biacore getting started kit 를이용한 KD 값도출방법 목차 1. 실험목적 2. 실험개요와필요한시약및소모품 2.1 실험개요 2.2 필요한시약및소모품 3. 실험방법과결과 3.1 ph-scouting for pre-concentration Title: Biacore getting started kit 를이용한 KD 값도출방법 목차 1. 실험목적 2. 실험개요와필요한시약및소모품 2.1 실험개요 2.2 필요한시약및소모품 3. 실험방법과결과 3.1 ph-scouting for pre-concentration 3.2 Immobilization 3.3 Regeneration scouting 3.4 Interaction

More information

Western Blot Consumable Best Seller Protein Standards Dual Color All Blue Kaleidoscope TM Dual Xtra Prestained Ch

Western Blot Consumable Best Seller Protein Standards Dual Color All Blue Kaleidoscope TM Dual Xtra Prestained Ch 2018 년 4 월 16 일 ~ 6 월 15 일까지 2018 비엠에스할 인 행 사 AbD Serotec 이 Bio-Rad 와하나가되었습니다. 10,000 여종의 Primary Antibody 를검색해보세요! Western Blot 장비 & 시약 5+2 증정 Western Blot Consumable Best Seller - Protein Standards -

More information

Oligo synthesis 4 단계공정을한 cycle 로하여 1 mer 씩 3' 말단에서 5' 말단까지순차적으로합성이진행됩니다. Deblocking Coupling Capping Oxidation OPC Purification Reverse-phase chromato

Oligo synthesis 4 단계공정을한 cycle 로하여 1 mer 씩 3' 말단에서 5' 말단까지순차적으로합성이진행됩니다. Deblocking Coupling Capping Oxidation OPC Purification Reverse-phase chromato 온라인용 Brochure 입니다. CUSTOM Oligo SYNTHESIS SERVICE Oligo synthesis 4 단계공정을한 cycle 로하여 1 mer 씩 3' 말단에서 5' 말단까지순차적으로합성이진행됩니다. Deblocking Coupling Capping Oxidation OPC Purification Reverse-phase chromatography

More information

2011 I Summer PLASTICS news HANWHA CHEMICAL CORPORATION http://hcc.hanwha.co.kr CONTENTS 04 10 31 45 70 74 85 2011 SUMMER 3 2011 Summer HANWHA NEWS 4 PLASTICS NEWS 2011 SUMMER 5 2011 Summer HANWHA NEWS

More information

Å©·¹Àγ»Áö20p

Å©·¹Àγ»Áö20p Main www.bandohoist.com Products Wire Rope Hoist Ex-proof Hoist Chain Hoist i-lifter Crane Conveyor F/A System Ci-LIFTER Wire Rope Hoist & Explosion-proof Hoist Mono-Rail Type 1/2ton~20ton Double-Rail

More information

- i - - ii - - iii - - iv - - v - - 1 - - 2 - - 3 - - 4 - - 5 - - 6 - - 7 - - 8 - - 9 - - 10 - - 11 - - 12 - - 13 - - 14 - - 15 - - 16 - - 17 - - 18 - - 19 - α α - 20 - α α α α α α - 21 - - 22 - - 23 -

More information

_....

_.... 2009 I Winter PLASTICS news HANWHA CHEMICAL CORPORATION http://hcc.hanwha.co.kr CONTENTS 04 08 24 42 50 62 74 2009 WINTER 3 2009 Winter HANWHA NEWS 4 PLASTICS NEWS 2009 WINTER 5 2009 Winter HANWHA NEWS

More information

Database Search 편 * Database Explorer 8개의카테고리로구성되어있으며, 데이터베이스의폴더역할을하는 subset ( 혹은 subbase) 을생성하여데이터를조직및관리하게된다. 클릭! DNA/RNA Molecules : feature map의데이터

Database Search 편 * Database Explorer 8개의카테고리로구성되어있으며, 데이터베이스의폴더역할을하는 subset ( 혹은 subbase) 을생성하여데이터를조직및관리하게된다. 클릭! DNA/RNA Molecules : feature map의데이터 Database Search 편 * Database Explorer 8개의카테고리로구성되어있으며, 데이터베이스의폴더역할을하는 subset ( 혹은 subbase) 을생성하여데이터를조직및관리하게된다. 클릭! DNA/RNA Molecules : feature map의데이터정보를 annotation하고, 다른소스로부터가져온데이터를 VectorNTI 내부포맷으로저장시킨다.

More information

ETC Electrolytic Technologies Corporation Electrolytic Technologies Corporation (ETC) (High Strength Sodium Hypochlorite). ETC.,. ETC,,. - (Cl2) (NaOH

ETC Electrolytic Technologies Corporation Electrolytic Technologies Corporation (ETC) (High Strength Sodium Hypochlorite). ETC.,. ETC,,. - (Cl2) (NaOH Innovator in Water Disinfection Solution 고농도차염 (NaOCl) 발생시스템 Klorigen K-Series Klorigen M-Series Generate Sodium Hypochlorite On-site and On-demand ETC Electrolytic Technologies Corporation Electrolytic

More information

Week01-workshop.hwp

Week01-workshop.hwp Polymerase Chain Reaction 과반응산물의정제 김상태서울대학교천연물과학연구소 adanson@plaza.snu.ac.kr A. Polymerase Chain Reaction Polymerase chain reaction (PCR) 은 genome 상의특정 DNA 염기서열을 denaturation, primer annealing, DNA polymerase에의한

More information

한약재품질표준화연구사업단 강활 ( 羌活 ) Osterici seu Notopterygii Radix et Rhizoma 생약연구과

한약재품질표준화연구사업단 강활 ( 羌活 ) Osterici seu Notopterygii Radix et Rhizoma 생약연구과 한약재품질표준화연구사업단 강활 ( 羌活 ) Osterici seu Notopterygii Radix et Rhizoma 생약연구과 - 2 - - 3 - KP 11 Osterici seu Notopterygii Radix et Rhizoma Ostericum koreanum Maximowicz, Notopterygium incisum Ting, Notopterygium

More information

<4D F736F F F696E74202D20454D49A3AF454D43BAEDB7CEBCC52EBBEABEF7BFEBC6F7C7D428BBEFC8ADC0FCC0DA >

<4D F736F F F696E74202D20454D49A3AF454D43BAEDB7CEBCC52EBBEABEF7BFEBC6F7C7D428BBEFC8ADC0FCC0DA > Materials Material Grades : PL Series Applicaton : Power transformer and Inductor Grades μi Freq. (MHz) Pcv 1) (Kw/ m3 ) Bs 2) (mt) Materials Characteristics PL-7 2,400 ~ 0.2 410 390 Mn-Zn Low loss PL-9

More information

슬라이드 1

슬라이드 1 New PCR Instruments 2013. 2. 21 다카라코리아바이오메디칼 Agenda FAST PCR이란? New FAST PCR Instrument _ TP450 New PCR Instrument _ TP350 기기캠페인소개 PCR FAST 관련 PCR 제품관련제품 최고수준의정확성 & 최고의신장속도 ( 신장성 5sec/kb) PrimeSTAR Max

More information

<C8B2BCBABCF62DB8D4B0C5B8AEBFCDB0C7B0AD2E687770>

<C8B2BCBABCF62DB8D4B0C5B8AEBFCDB0C7B0AD2E687770> 먹거리와 건강 황성수 인도주의실천의협의회(humandoctor.org) 황성수 의사님의 글 http://humandoctor.org/technote/main.cgi?board=whang 서적 곰탕이 건강을 말아먹는다 로도 출판되어 있음. lightworker.kr 편집 1. 이 글을 읽는 분들에게 황성수의 먹거리와 건강 을 읽는 모든 분들에게 인사드립니다.

More information