353-07(07-48).fm
|
|
|
- 은정 경
- 6 years ago
- Views:
Transcription
1 Kor. J. Microbiol. Biotechnol. Vol. 35, No. 3, (2007) z ww z Homocysteine wz, Cystathionine β-synthase yw p Ÿy*ÁxûÁ 1 Á 1 œw w ywœ, 1 w m y l Biochemical Characteristics for the Cofactor Free Mutant of Yeast Homocysteine Catalyzing Enzyme, Cystathionine β-synthase. Jhee, Kwang-Hwan*, Hyun-Nam Cho, Seun-Ah Yang 1, and In-Seun Lee 1. Dept. of Applied Chemistry, Kumoh National Institute of Technology, Gumi , Korea, 1 The Center for Traditional Microorganism Resources, Keimyung University, Daegu , Korea Mutations in the cystathionine β-synthase (CBS) gene cause homocystinuria, the most frequent inherited disorder in sulfur metabolism. CBS is the unique enzyme using both heme and pyridoxal 5-phosphate (PLP) for activity. Among the reported 140 mutations, one of the most common disease-causing alterations in human CBS is G307S mutation. To investigate the pathogenic mechanism of G307S by spectroscopic methods, we engineered the full length and the truncated G247S mutation of yeast CBS that is corresponding mutation to human G307S. Yeast CBS does not contain heme and thus gives a merit to study the spectroscopic properties. The UV-visible spectra of the purified full length and the truncated G247S yeast CBSs showed the total absence of PLP in the protein. The absence of PLP in G247S mutation was also confirmed by the PLP-cyanide adduct formation experiment, which was conducted by the incubation of the purified enzyme with KCN. The adducts were detected using a circular dichroism (CD) and a spectrofluorimeter. Radio isotope activity assay of full length and truncated G247S proteins also gave no activity. Our yeast G247S mutation data suggested that G307S might make the distortion of the active site so that cofactor PLP and substrate can not fit inside the active site. Our yeast CBS study addressed the reason why the G307S mutation in human CBS makes the enzyme inactive that consequently leads to severe clinical phenotype. Key words: Homocysteine, Cystathionine β-synthase, Mutant, PLP 1964 homocystinuriaš w (NIH) w w š[16]. cystathionine β-synthase(cbs) ü homocysteine w ƒ,, p. 30 w wù j ñ wù, homocysteine y y y» w x w w, yw ƒ t š [17, 20]. š, CBS y homocysteine xƒ ƒw xš [9, 13]. w homocysteine Alzheimer disease x w *Corresponding author Tel: , Fax: khjhee@kumoh.ac.kr» w» w[18]. L-Homocysteine y ww L-methionine L-cysteine ü v. ww L-homocysteine L- methionine py (re-methylation) jù L- serine ww L-cystathionine transsulfuration k homocysteine w jš. û homocysteine re-methylation wù homocysteine ƒ CBS w w (Fig. 1). CBS ywƒ l [8]. homocysteine w w w, CBS w y š. x 140ƒƒ CBS ƒ š, ƒ w clinical phenotype š[10, 12]. š G307S ƒ wš [5]. G307S w z (Saccharomyces cerevisiae)» z w ww
2 Fig. 1. Reaction scheme catalyzed by cystathionine β-synthase. Fig. 2. Homology between human CBS and yeast CBS. Numbers indicated the start amino acids and final amino acids that were compared. Shadow box indicated the identical amino acids. Symbol as + represents the similar amino acid for isoleucine and valine. Glycine residues for G307 of human and G247 of yeast are indicated and represented as bigger fonts. PLP-FREE MUTATION OF YEAST CYSTATHIONINE β-synthase 197 š w[11, 19]. CBS z CBS 1 BLASTP w alignment 62% š p ww y G307( ) G247(z) 93% p (Fig. 2), CBS G307 ww z CBS G247 serine eyw z G247S z w CBS x zyw ³wš w. wr CBS z heme pyridoxal 5 phosphate (PLP, k B 6 ) wwš, z CBS z PLP ƒš heme Ÿw f t PLP Ÿw p qw CBS z PLP» w, x, x z f ƒ Ÿ w ww[6]. wr CBS 551 N heme w(1-55)ƒ š 119 lysine»ƒ z PLP wwš. š 441 proteolysisƒ ù C ƒ w [7]. š (truncated) z z 2-3 w y ¾ N (Nterminal domain) (catalytic domain) tx wš ¾ C (C-terminal domain) (inhibitory domain) txwš. w S-adenosyl methionine(sam) C ww CBS y š. CBS heme PLP z wš hinge site ƒš w SAM w y x allosteric z[15]. z ƒƒ 3 x w C-terminal, heme, PLP, SAM w w w z w» w. z CBS 507 CBS 44 CBSƒ ƒš heme wƒ w» [14], z CBS 53 lysine»ƒ PLP wwš. š 354» proteolysisƒ w[7]. SAM w y [7]. z CBS z CBS heme w C SAM wƒ w. CBS w z CBS w z, 1, z PLP w š w»p w f œ w z CBS CBS wš w heme w Ÿw ¼w z[8]. CBS G307» ww z G247 serine ey w full-length z CBS (F-CBS) 354» C w truncated z CBS (T-CBS) w Ÿw y š z rš w. š š CBS 3 w clinical phenotype G307 3 e w zyw l (seriousness) š w. CBS w f wwš qw ª CBS y mw homocystinuria e. x L-cystathionine, L-serine, PLP, L-homocysteine thiolactone, SAM Sigma w. L-[U- 14 C]Serine (160 mci/ mmol) NEN Life Science w. Gigapite hydoroxylapatite, Seikagaku w. L-Homocysteine L-homocysteine thiolactone š w w[3]. L-Homocyteine
3 198 JHEE et al. w», 5,5 -dithiobis(2-nitrobenzoic acid) 412 nm Ÿ 13,600 M -1 cm w -1 w[4]. w Buffer B 50 mm Na/bicine 1 mm EDTA 1 mm DTT ww ph 7.8 bufferš Buffer K buffer 1 mm EDTA 1 mm DTT ww ph 7.5 buffer. z G247S l x x z CBS š» w[6, 7]. x full length CBS (F- CBS) pf-sec, truncated CBS(T-CBS) pf- SEC w. G247S PCR w ww quick change site-directed mutagenesis kit pfu DNA polymerase (Stratagene) w. w primer. Forward primer, 5 -GTTGAGGGTATT- TCTTATGATTTTGTTCC-3 ; Reverse primer, 5 -GGAACA- AAATCATAAGAAATACCCTCAAC-3. G247S-F pf-sec template wš G247S-T pt-sec template w. plasmid ww E.coli ƒw, pf-sec ATCC No , pt- SEC ATCC No x CBS(x x) x x š w [6, 7]. G247S x š w w wš G247S-F G247S-T z š w[6, 7]. zyd zy d ƒ w. C L-serine k universalw 14 k» w z k x L- cystathionine TLC w k Phosphor- Imager w w[6]. 1 unit 37 C w o g 1 mmol L-cystathionine xw y w.» L-serine L-homocysteine 5 mm w. yy(h 2 S) d w. CBS β-mercaptoethanol L-cysteine S-hydoroxyethyl-L-cysteine yyƒ x, yy lead acetate g lead sulfide (PbS) e xw, UV-visible ŸŸ 390 nm Ÿ dw[2, 7]. β- mercaptoethanol L-cysteine ƒƒ 30 mm w, lead acetate 0.4 mm w. lead sulfide (PbS) x Native-PAGE gel lead acetate k gel dw [2]. z PLP w d z PLP w Adams w[1]. r CBSz 5 mm buffer (ph 7.5) nw z, 11% trichloroacetic acid 50 o C 15 k z, 1.8 mm KCN 50 o C 25 k. x pyridoxal-p-cn excitation q 325 nm emission q420 nm dw. PLP w w w. Y=Y max [X]/K d + [X]», Y zy X PLP ùkü. PLP 0.1 N NaOH 388 nm PLP Ÿ e = 6.55 mm -1 cm w -1 w. Ÿ UV-visible rp Hewlett Packard 8453-diode array rp w. d j y 25 C o w w Peltier l w. CD r p Jasco J-175 w. š z x CBS G247S z x full length CBS (WT-F) truncated CBS (WT-T) š IPTG induction g wš 50% SDS-PAGE w w(data not shown). z G247S-F G247S-T, x šxwš x SDS-PAGE vj w(fig. 3). CBS e, column chromatography e ƒ SDS-PAGE mw ywš zy dw. zy d 1 w w x vwš TLC ww z, TLCq 12 radiography v w rw d 2, yy x lead acetate w dw w. ù G247S-F G247S-T 1 2 y SDS-PAGE w w. w x CBS š G247S-F G247S-T x w ƒ j ww. š G247S-F w j» 56 kda š, G247S-T 39 kda j
4 P Fig. 3. SDS-PAGE analysis of the purified yeast G247S proteins. Lanes; M, molecular mass standards (molecular mass in kda in parentheses) myosin (220), phosphorylase b (97), bovine serum albumin (72), ovalbumin (46), carbonic anhydrase (29), β-lactoglobulin (20), and lysozyme (15). 1, purified truncated form of G247S (G247S-T), indicating 39 kda; 2, purified full length form of G247S (G247S-F), indicating 56 kda. Yeast CBS mutants, G247S-F and G247S-T were purified from host cell XL-1 blue harboring mutated pf-sec and pt-sec. Each enzyme was purified using DEAE-Sephacel, Gigapite, and Butyl-Sepharose column chromatography. About 10 mg of protein was applied per lane. E.coli 기를 보였다(Fig. 3). 그리고 SDS-PAGE 상에서의 단백질이 정확히 CBS 단백질임을 확인하기 위하여 Fig. 3의 샘플의 N 말단순서를 Perkin-Elmer사의 protein sequencer를 이용하여 확인한 바 56 kda과 39 kda의 양 샘플 모두 M-T-K-S-E를 나타내었는데 이는 CBS의 N 말단의 다섯 아미노산의 순서 와 일치하므로 정제한 단백질이 CBS임이 확인되었다. 효모 G247S의 spectrum data 순수 정제된 야생형 CBS (WT-F)와 G247S (G247S-F)의 UV-visible 흡광도를 조사하였다. 야생형의 경우에는 보고된 바와 같이 280 nm와 412 nm에서 피크를 나타내었다. 280 nm의 피크는 단백질을 나타내고 412 nm의 피크는 CBS의 활성부위에 있는 lysine 잔기와 결합하고 있는 조효소 PLP 의 존재를 나타내고 있다. 그리고 그 비율이 280 nm : 412 nm = 1 : 0.152를 나타내었다(Fig. 4). 이는 보고된 수치 1 : 0.16과 매우 근사치를 나타내고 있으며 Fig. 3의 SDS-PAGE 의 결과와 더불어 CBS0.1%가 매우 순수함을 나타내고 있다. 280 nm에서의 WT-F의 A 280 값이 0.94이므로[7], Fig. 3의 단백 질의 농도는 정확히 1 mg/ml이 된다. 그리고 WT-T의 경우 는 WT-F의 흡수스펙트럼과 동일하였다(data not shown). 반 면 G247S-F의 경우에는 280 nm의 피크는 0.86으로 야생형 의 apo-enzyme의 경우와 정확히 일치하였다[7]. 즉 G247S 는 PLP를 함유하고 있지 않으며 야생형 CBS와는 다르게 412 nm에서 피크가 전혀 보이지 않았는데 이는 G247S-F가 조효소인 PLP를 전혀 함유하고 있지 않다는 증거이다. 이는 LP-FREE MUTATION OF YEAST CYSTATHIONINE β-synthase 199 Fig. 4. Absorption spectra of yeast CBS. Data exhibit the full length form of wild-type (WT-F, bold line) and full length form of G247S (G247S-F, dashed line). Each protein concentration was approximately 1 mg/ml in buffer B. All spectra were taken at 25oC. 왜 G247S가 전혀 활성을 보이지 않았는지에 대한 이유이기 도 하였다. L-homocysteine과 L-serine과의 반응에서 Lcystathionine 을 형성하는 반응이거나, β-mercaptoethanol과 L-cysteine과의 반응으로 S-hydoroxyethyl-L-cysteine과 황화 수소가 형성되는 반응이든 모두 PLP가 없으면 불가능하기 때문에 G247S는 촉매할 수 있는 반응이 없게 되는 것이다. G247S-T의 CBS의 경우에도 흡수스펙트럼을 측정하였으나 G247S-F의 CBS와 동일하였다(data not shown). 그리고 효 소에 기질인 L-serine을 넣어도 흡수spectrum에 영향이 없었 다. 이는 G247S-F가 기질과 결합하지 않음을 나타내고 있 다. 야생형의 경우에는 aminoacrylate 중간체의 형성으로 인 하여 460 nm 부근으로 흡수극대치가 이동한다[6]. 효모의 WT-F 와 WT-T, G247S-F와 G247S-T의 CD spectrum을 측정하였다. CD spectrum으로써 조효소, PLP의 함량을 알 수 있다[6]. 야생형의 CBS는 WT-F와 WT-T 모 두 412 nm 부근에서 (+)의 CD를 나타내지만 G247S-F와 G247S-T는 412 nm에서 CD를 보이지 않고 있다(Fig. 5). 이는 UV-visible 흡수스펙트럼과 같은 결과로써 G247S 변 이는 G247S-F와 G247S-T 모두 PLP를 함유하고 있지 않다 는 결과이다. 그리고 효소에 기질인 L-serine을 넣어도 CD spectrum에 아무 영향이 없었는데 이는 G247S 변이 단백질 이 기질과 전혀 반응을 하지 않음을 나타내고 있다. 야생형 의 경우에는 중간체의 형성으로 인하여 460 nm 부근에서 (-)의 CD를 보인다[6]. 그리고 야생형의 apo 단백질 역시 412 nm에서 CD를 보이지 않는다[6]. PLP 함량과 PLP와의 친화력 측정 G247S의 UV-visible과 CD spectrum의 결과를 보면
5 200 JHEE et al. Fig. 5. Circular Dichroism spectra of yeast CBS. Spectra exhibit the full length form of wild-type (WT-F), truncated form of wildtype (WT-T), full length form of mutant G247S (G247S-F), and truncated form of mutant G247S (G247S-T). Each protein concentration was 1 mg/ml in buffer K. All CD spectra were taken at 25 o C. G247S-F G247S-T PLPƒ x w. ù rp š PLP w dw. G247S trichloroacetic acid w g ü w PLP k z KCN j PLP-CN w ƒ š yw excitation q 325 nm emission q 420 nm xÿrp ùkü ü w PLP w. PLP Ÿ w w. WT-F WT-T ƒƒ w PLPƒ ƒš PLPƒ 1: 1 w. ù G247S-F G247-T ƒƒ PLPƒ. wr zy d2 w WT-F WF-T apo z PLP ƒj y dw PLP w (association constant), Ka dw WT-F 0.8 µm, WF-T 0.7 µm ùkü. ù G247S G247S-F G247S-T PLP (2 mm, x 2,500) zy 1 z y 2 y. G247S PLP ww. CBS z y CBS zy ƒ dw. L- Homocysteine kƒ C L-serine» 14 w z k z TLC mw C 14 L-cystathionine C û» 14 L- Fig. 6. CBS activity assay. The reaction mixture, which contained 200 mm Tris-HCl, ph 8.6, 20 mm PLP, 0.25 mg/ml bovine serum albumin, 5 mm L-[U- 14 C]serine (800 cpm/nmol), and CBS ( mg) in 18 ml, was preincubated for 5 min at 37 o C. The reaction was initiated by adding 2 ml of 5 mm L- homocysteine and was terminated after 10 min by adding 5 ml of 50% trichloroacetic acid. Reaction mixture was centrifuged for 3 min, and 5 ml of supernatant was applied to a cellulose thin layer chromatography plate (Kodak). The product, L-[ 14 C] cystathionine, was separated from L-[ 14 C] serine by ascending thin layer chromatography in 2-propanol/formic acid/h 2 O (80/6/20, v/v). Radioactivity of the product was determined by PhosphorImager (Molecular Dynamics) and the data was analyzed by AlphaImager HP software. The lanes are: C, L-[ 14 C] serine without CBS; 1, reaction with wild-type (WT-F); 2, reaction with truncated form of wild-type (WT-T); 3, reaction with full length form of mutant G247S (G247S-F); and 4, reaction with truncated form of mutant G247S (G247S-T). serine w z PhosphorImager L-cystathionine w(fig. 6). w L-homocysteine ƒ w PhosphorImager ùkù. spotalphaimager HPvp w x L-cystathionine z w control G247S-F, G247S-T y x š, WT-F WT-T ƒƒ 420 unit 780 unit» š w[7]. CBSyd β-mercaptoethanol L-cysteine S-hydoroxyethyl-L-cysteine x yy (H 2 S) dw. y G247S-F G247S-T x y. w Native-PAGE gel Pbacetate k z y dw G247S-F G247S-T x y (data not shown). w w yd, š PLPƒ w (2 mm¾ w) y x. G247S w G307S ƒ homocystinuria y, PLP n x y ƒ y ƒ
6 Fig. 7. Active site and G307 position in 3D structure of human CBS. Rasmol software was used and the human CBS 3-D coordination was from PDB code 1JBQ, human CBS[14]. Heme, PLP and active site lysine119 was represented. The distance between G307 residue and PLP was 7.2Å. w w. š G307S z y x (unpublished data). w G307S z complementation x x z CBS ew w[19]. zy w ww G307S z G247S w yw š G247S w Ÿw ƒ ƒw š PLP ƒ y. CBS G307 3 w x CBS 3 Rasmol vp mw y PLP lysine119 G307 e r. PLP lysine119 ε Schiff s base mw wwš N ew heme group PLP 11Å. G307 y PLP 7.2Å ù G307Sƒ PLP w x R group glycine w j R PLP-FREE MUTATION OF YEAST CYSTATHIONINE β-synthase 201 group ƒ serine ƒ ù PLPƒ y ö š ƒ. w» w w e š ƒ, w w bulky group» w m ww š. š ƒ» w j w G307 loop ew 3 y w w y. ƒ ƒw w CBS G307S 3 ww w š z CBS 3 x z x CBS G347S 3 z 3 y w w ƒ x z CBS» w w. x y x w homocysteine ü yj z cystathionine β-synthase w ww. cystathionine β-synthase ƒ» zy ƒ w homocysteine j homocystinuria» G307 serine ey ƒ w. wr cystathionine β-synthase heme prosthetic group ƒš rp ƒ ù,» š cystathionine β-synthase ƒ z cystathionine β-synthase heme swwš rp w w G307 ww G247 serine ey, w yw p r. z G247S C truncated form w full length form ƒ w xw.» L-homocysteine L-serine w C 14 w y dw y x β-mercaptoethanol L-cysteine» w yy w. w UV-visible spectrum CD spectrum PLP p 410 nm x w. w PLP KCN incubation x PLP w. š cystathionine β-synthase 3 w, G307 z PLP ew bulkyw R group serine ey z PLP w w y w ƒ. G247S š PLP incubationw y z PLP
7 202 JHEE et al. incorporation. G307Syƒ PLP nw y wš. G307S»w homocystinuria y CBS y w PLP n zƒ z w y. œw w ( ). REFERENCES 1. Adams, E Fluorometric determination of pyridoxal phosphate in enzymes. Method. Enzymol. 62: Chen, X., K. H. Jhee, and W. D. Kruger Production of the neuromodulator H 2 S by cystathionine β-synthase via the condensation of cysteine and homocysteine. J. Biol. Chem. 279: Drummond, J. T., J. Jarrett, J. C. Gonzalez, S. Huang, and R. G. Matthews Characterization of nonradioactive assays for cobalamin-dependent and cobalamin-independent methionine synthase enzymes. Anal. Biochem. 228: Ellman, G. L Tissue sulfhydryl groups. Arch. Biochem. Biophys. 82: Gallagher, P. M., P. Ward, S. Tan, E. Naughten, J. P. Kraus, G. C. Sellar, D. J. McConnell, I. Graham, and A. S. Whitehead High frequency (71%) of cystathionine β- synthase mutation G307S in Irish homocystinuria patients. Hum. Mutat. 6: Jhee, K. H., P. McPhie, and E. W. Miles Yeast cystathionine β-synthase is a pyridoxal phosphate enzyme but, unlike the human enzyme, is not a heme protein. J. Biol. Chem. 275: Jhee, K. H., P. McPhie, and E. W. Miles Domain architecture of the heme independent yeast cystathionine β- synthase provides insights into mechanisms of catalysis and regulation. Biochemistry. 39: Jhee, K. H. and W. D. Kruger The role of cystathionine β-synthase in homocysteine metabolism. Antioxid. Redox. Signal. 7: Kim, J., M. K. Park, E. Kim, C. Han, S. A. Jo, and I. Jo Plasma homocysteine is associated with the risk of mild cognitive impairment in an elderly Korean population. J. Nutr. 137: Kraus, J. P., M. Janosik, V. Kozich, R. Mandell, V. Shih, M. P. Sperandeo, G. Sebastio, R. de Franchis, G. Andria, L. A. Kluijtmans, H. Blom, G. H. Boers, R. B. Gordon, P. Kamoun, M. Y. Tsai, W. D. Kruger, H. G. Koch, T. Ohura, and M. Gaustadnes Cystathionine β-synthase mutations in homocystinuria. Hum. Mutat. 13: Kruger, W. D. and D. R. Cox A yeast system for expression of human cystathionine β-synthase: structural and functional conservation of the human and yeast genes. Proc. Natl. Acad. Sci. 91: Kruger, W. D., L. Wang, K. H. Jhee, R. H. Singh, and L. J. Elsas 2nd Cystathionine β-synthase deficiency in Georgia (USA): correlation of clinical and biochemical phenotype with genotype. Hum. Mutat. 22: Linnebank, M., A. Homberger, R. Junker, U. Nowak-Goettl, E. Harms, and H. G. Koch High prevalence of the I278T mutation of the human cystathionine beta-synthase detected by a novel screening application. Thromb. Haemost. 85: Meier, M., M. Janosik, V. Kery, J. P. Kraus, and P. Burkhard Structure of human cystathionine β-synthase: a unique pyridoxal 5'-phosphate-dependent heme protein. J. EMBO. 20: Miles, E. W. and J. P. Kraus Cystathionine β-synthase: structure, function, regulation, and location of homocystinuriacausing mutations. J. Biol. Chem. 279: Mudd, A. H., J. D. Finkelstein, and F. Irrevere Homocystinuria: an enzymatic defect. Science. 143: Pezzini, A., E. Del Zotto, and A. Padovani Homocysteine and cerebral ischemia: pathogenic and therapeutical implications. Curr. Med. Chem. 14: Seshadri, S., A. Beiser, J. Selhub, P. F. Jacques, I. H. Rosenberg, R. B. D'Agostino, P. W. Wilson, and P. A. Wolf Plasma homocysteine as a risk factor for dementia and Alzheimer's disease. N. Engl. J. Med. 346: Shan, X. and W. D. Kruger Correction of diseasecausing CBS mutations in yeast. Nat. Genet. 19: Wierzbicki, A. S Homocysteine and cardiovascular disease: a review of the evidence. Diab. Vasc. Dis. Res. 4: (Received Aug. 3, 2007/Accepted Sep. 9, 2007)
304.fm
Journal of the Korean Housing Association Vol. 20, No. 3, 2009 yw s w - û - A Study on the Planning of Improved-Hanok - Focused on Jeon-Nam Province - y* ** z*** **** Kang, Man-Ho Lee, Woo-Won Jeong, Hun
50(5)-07.fm
Printed in the Republic of Korea w 3w yû x w w w Á x* w w w yw (2006. 3. 14 ) A research of the Difference in Teaching Styles and Understanding of 9 th Grade Students About Lead-iodide Precipitation Reaction
16(1)-3(국문)(p.40-45).fm
w wz 16«1y Kor. J. Clin. Pharm., Vol. 16, No. 1. 2006 x w$btf3fqpsu'psn û w m w Department of Statistics, Chonnam National University Eunsik Park College of Natural Sciences, Chonnam National University
605.fm
Journal of the Korean Housing Association Vol. 19, No. 6, 2008 y k y p w sƒ Care-giver s Needs and Evaluation on the Actual Condition of the Playgrounds in Child Care Facilities y* Choi, Mock-Wha x **
82-01.fm
w y wz 8«( 2y) 57~61, 2005 J. of the Korean Society for Environmental Analysis p w w Á Á w w» y l Analysis of Influence Factors and Corrosion Characteristics of Water-pipe in Potable Water System Jae Seong
50(1)-09.fm
2006, Vol. 50, No. 1 Printed in the Republic of Korea 언빨래가마르는현상에대한중등학교화학전공교사들의인식조사 ½x Á» Á½ # Á x* w w yw y š w w # w w (2005. 5. 11 ) A Research of Secondary School Chemistry Major Teachers Perceptions
11(5)-12(09-10)p fm
w wz J. Kor. Soc. Cloth. Ind. 11«5y, 2009 Vol. 11, No. 5, pp.799-807(2009) w w * k ƒm w pg p w, ƒm w q w A Qualitative Research on Pursuing Image and Appearance Management Behavior of Brides Eun-Joo Bae
10(3)-09.fm
w y wz 10«3y 253~258 (2010.12.) Journal of Korean Society of Urban Environment ³ w Á» Á Á y w y œw (2010 11 22, 2010 12 9 k) Study on Determine of Detention Pond in Small Developed Area In-Soo Chang ½
49(6)-06.fm
Journal of the Korean Chemical Society 2005, Vol. 49, No. 6 Printed in the Republic of Korea 중학교과학교과서불꽃반응실험에서선스펙트럼관찰의문제점분석및개선연구 ½ Á Á * w w yw (2005. 10. 4 ) An Analysis and Improvement of the Line Spectrum
10(3)-10.fm
w y wz 10«3y 259~264 (2010.12.) Journal of Korean Society of Urban Environment w gj p p y Á Á½k * w m œw Á* w y œw (2010 9 28, 2010 10 12 k) Characteristics of Antiwashout Underwater Concrete for Reduction
14.531~539(08-037).fm
G Journal of the Korea Concrete Institute Vol. 20, No. 4, pp. 531~539, August, 2008 š x y w m š gj p { sƒ z 1) * 1) w w Evaluation of Flexural Strength for Normal and High Strength Concrete with Hooked
69-1(p.1-27).fm
99 A 380 B 787 : wœ z w * w w wœ» A380 B787 wœ» w. wœ» ww r. w wœ» p j y w r» w. I. II. z III. A380 B787 IV. A380 V. wœ» : x VI. z VII. I. A380 B787»ƒ z wœ w, w w p j w w ƒ r. t wœ w ƒ w w wœ» š œm wš,
19(1) 02.fm
Korean J. Crystallography Vol. 19, No. 1, pp.7~13, 2008 Ÿ (ICISS) w š t w (2): t w y w œw Surface Structure Analysis of Solids by Impact Collision Ion Scattering Spectroscopy (2): Atomic Structure of Semiconductor
15.101~109(174-하천방재).fm
w wz 8«4y 2008 8 pp. 101 ~ 109 w» m -,, - A Study on Warnning Criteria Investigation of Automated Rainfall Warning System -Focused on Realationship of Water Level, Discharge and Precipitation - Á Á Á Ahn,
< DC1A4C3A5B5BFC7E22E666D>
¼ (Jeong, Jung Chae)*, ý (Kim, Yoon Soo), (Shin, Woo Young), Þ Ñ (Park, Jong Man) ò ý ƒ Ð (Korea Evaluation Institute of Industrial Technology) (Shin, Jae-Heyg) Š æ (Ministry of Knowledge Economy) 1. :
10(3)-12.fm
w y wz 10«3y 273~280 (2010.12.) Journal of Korean Society of Urban Environment p yá xá½k w y œw (2010 9 15, 2010 12 2 k) Analysis of Characteristics of Delivered Nonpoint Source Pollution at Forested Watershed
9(3)-4(p ).fm
w wz J. Kor. Soc. Cloth. Ind. 9«3y, 2007 Vol. 9, No. 3, pp.319-326(2007) w xk x p w q w Analysis of Foot Characteristics According to the Classification of Foot Types of Junior High School Girls Ji-Young
DBPIA-NURIMEDIA
Printed in the Republic of Korea "/"-:5*$"- 4$*&/$& 5&$)/0-0(: Vol. 18, No. 5, 425-430, 2005» 677*4 Ÿ w sƒ ½»x Á Á½ k w y w, w y œw Some considerations for the analytical approaches to measure atmospheric
untitled
ª Œª Œ 27ƒ 2B Á 2007 3œ pp. 193 ~ 199 ª ƒ w d w ƒ sƒ Methodology of Drought Assessment Using National Groundwater Monitoring Network Data «x Á½ Kwon, Hyung JoongÁKim, Seong Joon Abstract The objective
12.077~081(A12_이종국).fm
J. of Advanced Engineering and Technology Vol. 1, No. 1 (2008) pp. 77-81 y w» e wx Á w œw Fabrication of Ceramic Batch Composition for Porcelain by Using Recycled Waste Ceramic Powder Hyun Guen Han, and
8(2)-4(p ).fm
w wz J. Kor. Soc. Cloth. Ind. 8«2y, 2006 Vol. 8, No. 2, pp.177-182(2006) x w e w 1) Á Ÿ 1) Á»û 2) 1) ƒm w q w 2) w w w The Effect of Media on Taking Plastic Surgery Chong-Hee Yun 1), Su-Kwang Sung 1) and
Statistical Data of Dementia.
Screening of Prolyl Endopeptidase Inhibitors from Medicinal Plants. Lee Si-young, Lee Jung-han, Paik Young-Sook College of Environment and Applied Chemistry, Kyung Hee University. Statistical Data of Dementia.
12(3) 10.fm
KIGAS Vol. 12, No. 3, September, 2008 (Journal of the Korean Institute of Gas) ü LP ƒ š Database w š Á Á½z w ywœw (2008 6 10, 2008 8 12 (1 ), 2008 9 5 (2 ), 2008 9 5 k) Constructing a Database Structure
27(5A)-07(5806).fm
ª Œª Œ 27ƒ 5A Á 2007 9œ pp. 753 ~ 758 gj pœw gj p { x A New Test Method for Pure Isotropic Flexural Tensile Strength of Concretes Ÿ Á y Á x Zi, GoangseupÁOh, HongseobÁChoi, Jinhyek Abstract Proposed is
50(4)-10.fm
2006, Vol. 50, No. 4 Printed in the Republic of Korea 칼코겐이도핑된망간산화물의저온합성연구 k Á z Á, *Áy, * y w ù w yw x ù l w» ww (2006. 7. 13 ) Chimie Douce Synthesis of Chalcogen-Doped Manganese Oxides Seung Tae Lim,GDae
17.393~400(11-033).fm
Journal of the Korea Concrete Institute Vol. 23, No. 3, pp. 393~400, June, 2011 GGGGG DOI 10.4334/JKCI.2011.23.3.393 x RC { sƒ y y 1) *Á½ 1) Á 2) 1) y w œw 2) w œw Bond Strength Evaluation of RC Beams
82-02.fm
w y wz 8«( 2y) 00~00, 2005 J. of the Korean Society for Environmental Analysis Calix[6]arene w k š w *Á x Á Á«ƒm w yw, *w w yw Cesium Ion Selective Solid Contact Electrodes Based on Calix[6]arene Won-sik
93.fm
Journal of the Korean Ceramic Society Vol. 45, No. 10, pp. 625~630, 2008. Effect of Storage Conditions on the Setting Properties of Brushite Bone Cement Containing Granular β-tricalcium Phosphate Sun-Ae
202.fm
Journal of the Korean Housing Association Vol. 19, No. 2, 2008 w w w w Physical Identities of Bukchon Hanok Area Viewed from Literary Geography * Park, Cheol-Soo "CTUSBDU This study explores the beneficial
416.fm
Journal of the Korean Housing Association Vol. 20, No. 4, 2009 œ qp œ y An Analysis of Change on the Apartment Unit Plans and the Interior Spaces Related to Women * Choi, ByungSook ** Park, JungA "CTUSBDU
16(2)-7(p ).fm
w wz 16«2y Kor. J. Clin. Pharm., Vol. 16, No. 2. 2006 ü t xy y w tœ ½ BÁ x BC B y w w w C y w w w Current Status and Expectations of Orphan Drugs in Korea -In point of supplying medicines for the rare
fm
[ ] w wz DOI: 10.3740/MRSK.2009.19.12.692 Kor. J. Mater. Res. Vol. 19, No. 12 (2009) y INCONEL 718w Gas Tungsten Arc Welding» p sƒ ½»y Á *Á *Á y** ( ) d lj p wœq, *w wœ» q **( ) d lj p t Mechanical Properties
51(4)-13.fm
Journal of the Korean Chemical Society 2007, Vol. 51, No. 4 Printed in the Republic of Korea w x - w y *Á š w w w yw (2007. 3. 28 ) Case Study on Verbal Interactions of Teacher-Small Group StudentsG in
untitled
[ ] œwz, 21«6y(2008) J. of the Korean Society for Heat Treatment, Vol. 21, No. 6, (2008) pp. 300~306 š y w p x*, **Á **Áy y* * ** w œ w œw, w» gœ Solid State Diffusion Brazing of the Aluminum Alloy Castings
07.051~058(345).fm
w wz 8«3y 2008 6 pp. 51 ~ 58 m qp yp š w k sƒ Evaluation of Dynamic Modulus based on Aged Asphalt Binder y*á **Á***Á**** Lee, Kwan-HoÁCho, Kyung-RaeÁLee, Byung-SikÁSong, Yong-Seon Abstract Development
w w l v e p ƒ ü x mw sƒw. ü w v e p p ƒ w ƒ w š (½kz, 2005; ½xy, 2007). ù w l w gv ¾ y w ww.» w v e p p ƒ(½kz, 2008a; ½kz, 2008b) gv w x w x, w mw gv
ª Œª Œ 30ƒ 5A Á 2010 9œ pp. 475 ~ 484 gj p ª v e p p PSC ƒ gv : II. x w Precast Concrete Copings for Precast Segmental PSC Bridge Columns : II. Experiments and Analyses ½kzÁ½ Á zá x Kim, Tae-HoonÁKim,
10(3)-02.fm
w y wz 10«3y 203~211 (2010.12.) Journal of Korean Society of Urban Environment w ù p yá k«áyw *Á xá½k w y œw Á* y w (2010 9 15, 2010 12 2 k) Characterization of Nonpoint Source Pollutant Loads from the
<30312DC0CCC7E2B9FC2E666D>
균류의다양성과역할 이향범 û w œw 머리말 k w š ³ q š yw < Ð >» w ww k w w v š q w Á Á w tyw š 2QKPVKPI CPF *[FG ú ƒ x %QPXGPVKQP QP $KQNQIKECN &KXGTUKV[ %$&» w w ««wš y w» š x ƒ w œ ƒ» ƒ œsw w ³ wš ù š y š ƒ t š ƒ wš»ƒ
10(1)-08.fm
w y wz 10«( 1y) 47~52, 2007 J. of the Korean Society for Environmental Analysis œ w t y ½ xá Á x Á½ Á x* Ÿ œ, * w œw Optimization of Coagulation In The Conventional Water Treatment Plant Jun-Hyun Kim,
12(2)-04.fm
J. Korean. Soc. Living. Environ. Sys. Vol. 12, No. 2, pp 106~111(2005) w y y w z œ k üœ» p ½ Á Á Á ³ Á Á½Ÿ w w w, *g», ** w w Effects of Strategies to Improve Indoor Air Quality at Pre-occupancy Stage
01.01~08(유왕진).fm
JOURNAL OF KOREA SOCIETY OF PACKAGING SCIENCE & TECHNOLOGY. Vol. 14, No. 1 1~8 (2008) s w Á w» w Comparative Study on the Changes and Prospects of Flexible Food Packaging Design Kyung Soo Noh and Wang
51(2)-09.fm
2007, Vol. 51, No. 2 Printed in the Republic of Korea xw yw w w» e w z Á Á y Áw # Á k * w yw w w w w w # w w w (2006. 10. 30 ) Influences of Current Education Programs for Preservice Chemistry Teachers
슬라이드 1
ARCHITECT i2000 를이용한 HOMOCYSTEINE 정량분석평가 짂단검사의학과생화학파트이유희 순서 Ⅰ. 서론 1) Homocysteine 의이해및소개 2) 시약교체배경 Ⅱ. 본론 1) 재료및방법 2) 결과 Ⅲ. 고찰 I. 서론 Homocysteine 메티오닌의이화작용 (catabolism) 에의해생성되는황을함유하는아미노산 혈장호모시스테인의수치는다음에영향을받는다.
<30332DB9E8B0E6BCAE2E666D>
kœw» y w k p y w k» z wš ye z w mdkqvqrg üœ» y» Ÿ w kœwgeqgpikpggtkpiy glj GEQVGEJPQNQI[ œw w k w yz š y j œ w m w y ˆ mw y» kœ w k û ³ y ƒ ƒ» ƒ w œw w w y ƒƒ» k z w» %NGCP 9CVGT #EV wšw y ù» œ w w w t
51(2)-06.fm
Journal of the Korean Chemical Society 2007, Vol. 51, No. 2 Printed in the Republic of Korea f p ƒ š w er elk yw ½ Á y*» l, w w ywœw (2006. 12. 5 ) Separation of Caffeine and Catechin Compounds from Green
14.fm
Journal of the Korean Ceramic Society Vol. 44, No. 2, pp. 93~97, 2007. Preparation of High Purity Si Powder by SHS Chang Yun Shin, Hyun Hong Min, Ki Seok Yun, and Chang Whan Won Engineering Research Center
18211.fm
J. of the Korean Sensors Society Vol. 18, No. 2 (2009) pp. 168 172 p k ù p p l xá xá ³ Á *Á w * Fabrication of the CNT-FET biosensors with a double-gate structure Byunghyun Cho, Byounghyun Lim, Jang-Kyoo
26(3D)-17.fm
26ƒ 3D Á 2006 5œ pp. ~ ª y w qp yw k d Predictive Equation of Dynamic Modulus for Hot Mix Asphalt with Granite Aggregates yá½x Á Lee, Kwan-HoÁKim, Hyun-OÁJang, Min-Seok Abstract The presented work provided
<312D303128C1B6BAB4BFC1292E666D>
k Ÿy y y + ûz m Ì ˆw k Ÿ ø ky w y y» wk Ÿ v w k w w ƒ Ÿ ew k Ÿy yø k Ÿ ý k z» w ƒ w Ÿ y k y w x mw w w ³Ÿ wšy v mw y w r œw yÿ ý w z»ÿ Ÿ»» Ÿ ¾ Ÿ 6TCXGN 9GGMN[ ýw k Ÿ Ÿ ƒ š wš y w k Ÿ ƒ m ³ w w y y y 'EQVQWTKUO
DBPIA-NURIMEDIA
Printed in the Republic of Korea "/"-:5*$"- 4$*&/$& 5&$)/0-0(: Vol. 18, No. 5, 436-443, 2005,1"$ w Q) t p z Á 1w w œw 2 w œ yw Effects of surface properties and solution ph on the pollutants removal of
10.063~070(B04_윤성식).fm
J. of Advanced Engineering and Technology Vol. 1, No. 1 (2008) pp. 63-70 Mg-3%Al-1%Zn w ƒœ» y Á x*á **Á Á w œw *w s l I ûer ** t Effect of Thermomechanical Treatment on Microstructure and Mechanical Properties
, 66~67dB»e 55dB š 12dBù û»e(65db) w 70~71dB ñ. ù ü»» 35dB(ü), 45dB() r. w» w 1938 œk ³Ø w, 1960 Ø, 1968 ³Ø w. w 1972 ³Ø w w ³ ƒwš, ù y Ø w ³w
ª Œª Œ 26ƒ 1D Á 2006 1œ pp. 1~11 ª qp md w An Analysis of the Traffic Noise Measurement Plans of Apartment Complexes A Case on the North Riverside Expressway in Seoul Á Kang, Jun MoÁLee, Sung Kyung Abstract
415.fm
Journal of the Korean Housing Association Vol. 20, No. 4, 2009 xw z œ p w A Study on the Spatial Characteristics of the Concept of Non-sedentary in Contemporary Multi-housing Planning ½ * Kim, Mi-Kyoung
21(4)-02.fm
J. Fd Hyg. Safety 21(4), 204 212 (2006) ŸŸ w» ƒ, ƒ, w ½ Á Á½ ¼Á½ Á Á½ Ášy Á» y Quantitative Analysis of Acid Value, Iodine Value and Fatty Acids Content in Sesame Oils by NIRS Jae-Kwan Kim, Myung-Jin Lee,
82.fm
Journal of the Korean Ceramic Society Vol. 44, No. 9, pp. 524~528, 2007. Determination of Critical Chloride Content of Ordinary Portland Cement Concrete by Linear Polarization Technique Hong-Sam Kim, Hai-Moon
( )-83.fm
Journal of the Korean Ceramic Society Vol. 8, No. 1, pp. 0~5, 011. DOI:10.191/KCERS.011.8.1.00 Solidification of Heavy Metal Ions Using Magnesia-phosphate Cement HunG Choi, Hyun Ju Kang, Myung Shin Song,
84-01.fm
w y wz 8«( 4y) 00~00, 2005 J. of the Korean Society for Environmental Analysis HPLC f y e t p»wá½»x w y w»y Detection Characteristics of a HPLC Method on Carbonyl-DNPH Derivatives in Relation with Column
49(6)-03.fm
Journal of the Korean Chemical Society 2005, Vol. 49, No. 6 Printed in the Republic of Korea 근적외선분광법을이용한새로운비파괴적계란신선도측정방법의개발에관한연구 ½ y*á Á Á Á z Á p Á Á½z ƒ rp lj w w (2005. 7. 5 ) Non-Destructive Measurement
fm
J. Fd Hyg. Safety 20(1), 53 57 (2005) Ÿ w y, w s z w Á½ xá Á Á yá v w t œw Antioxidative, Antimutagenic and Cytotoxic Effects of the Mineral Water Seung-shi Ham, Soo-hyun Kim, Seon-young Moon, Mi-Sun Jeon,
(2)-02(최경자).fm
Res. Plant Dis. 16(2) : 148-152 (2010) Research in Plant Disease The Korean Society of Plant Pathology z w t w sƒ Á½ Á Á yá * w yw yw l Development of Effective Screening Method and Evaluation of Radish
16(5)-06(58).fm
Journal of Korean Powder Metallurgy Institute DOI: 10.4150/KPMI.2009.16.5.336 y-y w Sm-Fe w ƒ w zá *Á w»» The Effect of Excess Samarium Oxide on the PreparationG of Sm-Fe Alloy Powder by Reduction-diffusion
50(6)-03.fm
Journal of the Korean Chemical Society Printed in the Republic of Korea w yw w w ½ Á Áw Á k * w yw w w w w w (2006. 8. 9 ) Relationship between Conceptual Understanding and Mapping Errors Induced in Learning
32(4B)-04(7455).fm
32«4ByÁ 2012 7 pp. 233 ~ 242 œ w w w y œ r Estimation of Domestic Water Supply Benefit Using Demand Function Approach ³ Á Á½¼yÁ Yeo, Kyu DongÁYi, Choong SungÁKim, Gil HoÁLee, Sang Won Abstract In the past,
07.045~051(D04_신상욱).fm
J. of Advanced Engineering and Technology Vol. 1, No. 1 (2008) pp. 45-51 f m s p» w Á xá zá Ÿ Á w m œw Image Retrieval Based on Gray Scale Histogram Refinement and Horizontal Edge Features Sang-Uk Shin,
Microsoft Word - KSR2013A299
YWXZ º º t rzyywxzhy`` k Ž Ÿo r m ƒ 2 Flanged uide Wheels Tram Review Compliance Criteria of the Road Structure Ò ã ä ã ä ãã Kwang-Suk Seo *, Seung-Il Lee *, Oak-Young Song * * "CTUSBDUTram of the city
fm
J. Fd Hyg. Safety 20(4), 253 257 (2005) ü w w w Áy»xÁ Á Á Á«Á Áw Á x Á yx Á½ Á½ Á½ t t» t t ƒ q Study on Management of Artificial Flavors in Korea Tal-Soo Lee, Young-Mi Jang, Ki-Hyoung Hong, Sung-Kwan
untitled
6ƒ 3A Á 006 5œ pp. 497 ~ 5 ª sƒ w k w w Improved Modal Pushover Analysis of Multi-span Continuous Bridge Structures z Áy Á½ Kwak, Hyo-GyoungÁHong, Seong JinÁKim, Young Sang Abstract In this paper, a simple
12(4) 10.fm
KIGAS Vol. 12, No. 4, December, 2008 (Journal of the Korean Institute of Gas) l x CNG» v m s w ½ Á y w» œw (2008 9 30, 2008 12 10, 2008 12 10 k) Numerical Analysis for Temperature Distribution and Thermal
50(6)-09.fm
Journal of the Korean Chemical Society 6, Vol. 5, No. 6 Printed in the Republic of Korea w Polyacrylamide y ½ û * w yw (6. 9. 1 Thixotropic Properties of Polyacrylamide Hydrogels with Various Synthetic
w wƒ ƒw xù x mw w w w w. x¾ w s³ w» w ƒ z š œ Darcy-Weisbach œ w ù, ù f Reynolds (ε/d) w w» rw rw. w w š w tx x w. h L = f --- l V 2 Darcy Weisbach d
ª Œª Œ 29ƒ 4B Á 2009 7œ pp. 357 ~ 363 ª pv w ü s w A Study on The Velocity Distribution in Closed Conduit by Using The Entropy Concept kyáeá½á Choo, Tai HoÁOk, Chi YoulÁKim, Jin WonÁMaeng, Seung Jin Abstract
(Establishment and Management of Proteomics Core Facility)
(Establishment and Management of Proteomics Core Facility) 1 sample running on SDS-PAGE Gel (10well) 163570 10 16357 1D size marker 225500 100 2255 Buffer(20X, 500ml) 119900 20 5995 Staining
14(4) 09.fm
J. Korean. Soc. Living. Environ. Sys. Vol. 14, No. 4, pp 343~350(2007) w y y w z 14 «4 y 2007 yw» l s p»xá ³ *w w w œw, **w w w The Property of Pressure Distribution of Hybrid Underfloor Air Distribution
한 fm
Journal of the Korean Magnetics Society, Volume 19, Number 6, December 2009 DOI: 10.4283/JKMS.2009.19.6.203 BaTi 0.5 Co 0.5 Fe 11 O 19 Mx r p Ka- q p ½ Á½ w, œw, w», 12, 361-763 (2009 8 4, 2009 9 10, 2009
93-09.fm
w y wz 9«( 3y) 199~206, 2006 J. of the Korean Society for Environmental Analysis sƒ(i) ½» *Áx Á * w yw w, w y œw Evaluation of Manual and Automated Methods in Total Nitrogen (TN) and Total Phosphorus (TP)
3.fm
Journal of the Korean Ceramic Society Vol. 44, No. 1, pp. 12~17, 2007. A Study on the Preparation of Bone Ash and Celadon Bone Body Using Pig Bone Jae-Jin Jeong, Sang-Hee Lee, Yong-Seok Lee,* and Byung-Ha
38(6)-01.fm
y, 38«, 6y, 699-705, 2005 Econ. Environ. Geol., 38(6), 699-705, 2005 t sÿ y xk 1 Á s 2Á z 1 Á½ 1 Á e 3 1 w y w,» w, 2w y w, 3š w y w Chemical Speciation of Heavy Metals in Geologic Environments on the
Microsoft Word - KSR2012A021.doc
YWXY G ºG ºG t G G GGGGGGGGGrzyYWXYhWYXG Ÿƒ Ÿ ± k ¹Ÿˆ Review about the pantograph field test result adapted for HEMU-430X (1) ÕÕÛ äñ ã G Ki-Nam Kim, Tae-Hwan Ko * Abstract In this paper, explain differences
Y 1 Y β α β Independence p qp pq q if X and Y are independent then E(XY)=E(X)*E(Y) so Cov(X,Y) = 0 Covariance can be a measure of departure from independence q Conditional Probability if A and B are
84-07.fm
w y wz 8«( 4y) 00~00, 2005 J. of the Korean Society for Environmental Analysis CWPO œ w 1,4- s Á Á * û y, * w yœ lœw 1,4-Dioxane Treatment using Catalytic Wet Peroxide Oxidation Process Jong-min Kang,
57.fm
Journal of the Korean Ceramic Society Vol. 45, No. 6, pp. 336~344, 2008. Rheological Properties of Cement Paste Blended Blast Furnace Slag or Fly Ash Powder Jong Taek Song, Hyo Sang Park, Seung Ho Byun,
11(1)-15.fm
w y wz 11«1y 73~80 (2011.8.) Journal of Korean Society of Urban Environment SWMM 을이용한도시지역비점오염부하저류시설용량산정 Áw«Á *Áy **Á½ yá½ Á x w w Á* û w y œw Á** w y lœw (2011 6 13, 2011 6 27 k) Determine Capacity of
04-46(1)-06(조현태).fm
w œwz, 46«1y 2009 Textile Science and Engineering Vol. 46, No. 1, 2009 ù 2'6 wx xk Á «w œ w» Áq œw k 1PG $CVJ1PG 5VGR &[GKPI H 0[NP2'6 5RNKV 6[RG /KETHKDGT *[GP 6CG %J CPF%JWN-YP2CTM &GRCTVOGPV H 1TICPKE
27(5A)-15(5868).fm
ª Œª Œ 27ƒ 5A Á 2007 9œ pp. 701 ~ 711 ª x 3 k w Radial 3-D Elastodynamic Infinite Elements Á Á½ Seo, Choon-GyoÁYun, Chung-BangÁKim, Jae-Min Abstract This paper presents new three dimensional radial infinite
17(2)-00(268).fm
w ywz 17«2y(2012)/ 1 AMESim» CRDi l 2 p w if * Á le * Áfld ** Analysis of Dynamic Characteristics in Two-stage Injection for CRDi Injectors Based on AMESim Environment Insu Jo, Jiwon Kwon and Jinwook Lee
15(2)-07.fm
J. Korean. Soc. Living. Environ. Sys. Vol. 15, No. 2, pp 145~154(2008) w y y w z 15 «2 y 2008 ³ l w sm ½ Á yáy * w y w, ** w w Recommended Sensitivity of a Fully-shielded Photosensor for a Daylight Dimming
01 Buffers & Gel Stain Buffers 3 Gel Stain SilverStar Staining Kit 6
Buffers & Gel Stain Chemicals Buffers & Chemicals Phone: 1588-9788 (ext.4->2) Email: reagents-support@bioneer.co.kr 01 Buffers & Gel Stain Buffers 3 Gel Stain SilverStar Staining Kit 6 Buffers Overview
14.091~100(328-하천방재).fm
w wz 8«4y 2008 8 pp. 91 ~ 100 w w w e x Development of a Numerical Model of Shallow-Water Flow using Cut-cell System ½x Á Á Kim, Hyung-JunÁee, SeungOhÁCho, Yong-Sik Abstract Numerical implementation with
THE JOURNAL OF KOREAN INSTITUTE OF ELECTROMAGNETIC ENGINEERING AND SCIENCE. vol. 29, no. 10, Oct ,,. 0.5 %.., cm mm FR4 (ε r =4.4)
THE JOURNAL OF KOREAN INSTITUTE OF ELECTROMAGNETIC ENGINEERING AND SCIENCE. 2018 Oct.; 29(10), 799 804. http://dx.doi.org/10.5515/kjkiees.2018.29.10.799 ISSN 1226-3133 (Print) ISSN 2288-226X (Online) Method
17(1)-06.fm
Krean J. Crystallgraphy Vl., N. 1, pp.14~18, 006 LP-MOCVD w ZnO ù Ÿw p Á yá * w w» w» l Structural and Optical Prperties f ZnO Nanwires Synthesized by LP-MOCVD Prcess Yung-Jin Chi, Jae-Hwan Park and Jae-Gwan
8(3)-15(p ).fm
w wz J. Kor. Soc. Cloth. Ind. 8«3y, 2006 Vol. 8, No. 3, pp.357-362(2006) w p k sƒ ½ Á Á w w w A Study on the Mechanical and Hand Properties of the Lining Fabrics Myung-Ok Kim, Mi-Kyung Uh and Myung-Ja
62.fm
Journal of the Korean Ceramic Society Vol. 45, No. 7, pp. 395~400, 2008. Preparation and its Characteristics of Fly Ash-based Geopolymeric Mortar using Low Grade Silica Waste Se Gu Son, Seung Yeob Hong,
06.177~184(10-079).fm
Journal of the Korea Concrete Institute Vol. 23, No. 2, pp. 177~184, April, 2011 GGGGG DOI 10.4334/JKCI.2011.23.2.177 x w w MRS w p s y 1) Á z 2) Á x 3) * 1) wû w œw 2) w œw 3) w Nonlinear Analysis for
25(6)-21(김유곤).fm
Printed in the Republic of Korea "/"-:5*$"- 4$*&/$& 5&$)/0-0(: Vol. 25, No. 6, 483-491, 2012 http://dx.doi.org/10.5806/ast.2012.25.6.483 Study on stability test of in process sample of recombinant Protein
64.fm
Journal of the Korean Ceramic Society Vol. 44, No. 7, pp. 375~380, 2007. Tribological Properties of Carbon Layers Produced by High Temperature Chlorination in Comparison with DLC Coating Hyun-Ju Choi,
4.fm
Journal of the Korean Ceramic Society Vol. 46, No. 1, pp. 30~34, 2009. Optimization of Glass Wafer Dicing Process using Sand Blast Won Seo, Young-mo Koo*, Jae-Woong Ko**, and Gusung Kim Department of Electronic
16(5)-04(61).fm
Journal of Korean Powder Metallurgy Institute DOI: 10.4150/KPMI.2009.16.5.326 ƒ w Fe œ w Cu wy SPS (II) II. w SPS p ½ *Á½ Á½{ a Á½ š b w œw, a w» gœ, b y œ Production of Fe Amorphous Powders by Gas-Atomization
45(3)-6(040)p fm
The Korean Journal of Microbiology, Vol. 45, No. 3, September 009, p. 63-67 Copyright 009, The Microbiological Society of Korea RpoS ³ ³ ƒ 1, Á½ ³ 1 * w w w w s» p RpoS w s w x w. ³ proline w w proba proc
11(4)-13(09-12)p fm
w wz J. Kor. Soc. Cloth. Ind. 11«4y, 2009 Vol. 11, No. 4, pp.633-640(2009) 20Á30 û v k x k y ½z **Á* w w **, w w * Man's Hair style Preference according to Lifestyle in Age 20's and 30's Hyo-Jung Kim**
9(2)-12(p ).fm
w wz J. Kor. Soc. Cloth. Ind. 9«2y, 2007 Vol. 9, No. 2, pp.167-175(2007) 30 q q f œ w q œw œ Transition of Fashion Trends and Fashion Colors in Recent 30 Years Kyung-Hee Lee Dept. of Textile & Fashion
Journal of Educational Innovation Research 2018, Vol. 28, No. 4, pp DOI: * A Research Trend
Journal of Educational Innovation Research 2018, Vol. 28, No. 4, pp.295-318 DOI: http://dx.doi.org/10.21024/pnuedi.28.4.201812.295 * A Research Trend on the Studies related to Parents of Adults with Disabilities