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1 45, (208) Korean J. Poult. Sci. Vol.45, No., (208) Dicky Tri Utama Effect of Liquid Smoke and Curing Mixture on Quality Characteristics of Chuncheon Dakgalbi during Storage Hae Seong Jeong, Ki Ho Baek 2, Dicky Tri Utama, Jun Tae Kim and Sung Ki Lee Animal Products and Food Science Program, College of Animal Life Sciences, Kangwon National University, Chuncheon 2434, Republic of Korea 2 Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea ABSTRACT The goal of the present work was to determine the optimal addition amounts of liquid smoke and curing mixture to develop Chuncheon Dakgalbi with improved preference and shelf-life. In the first experiment, Chuncheon Dakgalbi was prepared with different amounts of liquid smoke. In the second experiment Chuncheon Dakgalbi was prepared with various amounts of curing mixture and 0.% (w/w) liquid smoke. Different amounts of liquid smoke resulted in different aroma patterns, which were observed using an electronic nose, and Dakgalbi with 0.% (w/w) liquid smoke had the highest score in overall acceptability. The addition of liquid smoke and curing mixture inhibited the growth of bacteria, slowed down the decline in ph, and delayed increased in volatile basic nitrogen contents and lipid oxidation. However, no clear effects were observed on instrumental color. From a consumer preference test, the highest preference score was achieved by added 0.2% (w/w) curing mixture. Liquid smoke and curing mixture extended shelf-life and improved preference of Chuncheon Dakgalbi. Considering the physicochemical, microbiological and consumer preference, it was recommended to add 0.% (w/w) liquid smoke and 0.2% (w/w) curing mixture to Chuncheon Dakgalbi to enhance shelf-life and preference. (Key words: Chunchen Dakgalbi, shelf-life, consumer preference, liquid smoke, curing mixture),. HMR(Home Meal Replacement) (Lee et al., 205). HMR. 2,,.,,,,,, (liquid smoke) (wood vinegar) (Kim et al., 998). Seo et al.(2000), Jeong and Shim(2002). Kim et al.(99), Gheisari et al.(207) Escherichia coli O57:H7 Listeria monocytogenes Escherichia coli O57:H7 Listeria monocytogenes. To whom correspondence should be addressed : skilee@kangwon.ac.kr

2 30 :.,.. ph,,.,, ph values, (Thiobarbituric Acid Reactive Substances; TBARS), (Volatile Basic Nitrogen; VBN),,.. 30 (Ross broilers) (Mireuchuksan Corp., Gyeonggi, Korea) %, 6%, 3.4%, 6%, 0.7%, 6.4%, 6.3%, 20%, 7%, 0.2%, 6%, 3.7%, L-.0% 5. (Hickory smoke oil RS-30, ES Food Co. Ltd, Gyeonggi, Korea) 5.0% 95.0% 0.2%. (Taewon Food Industry Co. Ltd, Gyeonggi, Korea) 93.%, 5.9%,.0%. 0.05%, 0.0%, 0.20% 30% Control, S-0.05( 0.05%), S-0.0( 0.0%), Table. Ingredient of Chuncheon Dakgalbi made with different amounts of liquid smoke Ingredient (%) Control S-0.05 S-0.0 S-0.20 Meat Total Dried red pepper powder Minced onion Minced garlic Minced ginger Soy sauce Milk Curry powder Refined sugar Soju Ground black pepper Starch syrup Ketchup Monosodium glutamate Liquid smoke Control: no liquid smoke, S-0.05: added 0.05% liquid smoke, S-0.0: added 0.0% liquid smoke, S-0.20: added 0.20% liquid smoke. 2 Meat: deboned drumette of 30-day-old Ross broiler. 3 Liquid smoke: smoke extract 5% and soybean oil 95%. S-0.20( 0.20%), Table. 50 (Convotherm 4 easydial GS, Eglfing, Germany) g Polyethylene (Jinwoong Chemical, Gyeonggi, Korea) (IV-2000, Incoin Tech, Gyeonggi, Korea) ph values, 48.

3 Jeong et al. : Effect of Liquid Smoke and Curing Mixture on Quality of Chuncheon Dakgalbi 3 30%, 0.%, 0.2% 0.3% (Convotherm 4 easydial GS) g (Jinwoong Chemical) (IV-2000). Control, S( 0.%), SC-0.2( 0.% + 0.2%), SC-0.3( 0.% + 0.3%), Table ph values,, TBARS, VBN, 0, 7, 4, 2, 28, 33, 47 56, , 3. ph values, TBARS, VBN, 3. 3 CIE L*(lightness), a*(redness), b*(yellowness) (CR-400, Konica Minolta Sensing, Inc., Japan) 4 5. calibrate plate(2 observer) illuminant C Y=93.6, x=0.334, y= ph g 45 ml homogenizer(ph9, SMT Co., Ltd., Japan) 2,000 rpm ph meter(seveneasy ph, Mettler-Toledo GmbH, Switzerland) g vial PTFE/rubber septa autosampler(hs00, Alpha MOS, Toulouse, France) 60 Table 2. Ingredient of Chuncheon Dakgalbi made with different amounts of liquid smoke and curing mixture Ingredient (%) Control S SC-0.2 SC-0.3 Meat Total Dried red pepper powder Minced onion Minced garlic Minced ginger Soy sauce Milk Curry powder Refined sugar Soju Ground black pepper Starch syrup Ketchup Monosodium glutamate Liquid smoke Curing mixture Control: no additive, S: added 0.0% liquid smoke, SC-0.2: added 0.0% liquid smoke and 0.20% curing mixture, SC-0.3: added 0.0% liquid smoke and 0.30% curing mixture. 2 Meat: deboned drumette of 30-day-old Ross broiler. 3 Liquid smoke: smoke extract 5% and soybean oil 95%. 4 Curing mixture: sodium chloride 93.%, sodium nitrite 5.9% and sodium carbonate.0%. 500 rpm 0, headspace auto sampler syringe(45 ) 2.5 ml 2 metal oxide injector (carrier gas flow rate air 50mL/min). principal component analysis(pca, Alpha soft version 8.0 software, Alpha MOS, Toulouse, France). 2 MOS(Metal Oxide Sensor) SY/LG, SY/G, SY/AA, SY/Gh, SY/gCT, SY/gCT, T30/, P0/, P0/2, P40/, T70/2 PA2, SY, T P Table 4. SY/LG P40/ fluoride chloride, SY/G,

4 32 : SY/Gh, SY/gCT ammonia sulphur, SY/AA, PA2 T30/ Organic solvents, SY/gCT, P0/ P0/2 nonpolar volatiles, T70/2 (Anonymous, 200). 3 2, TBARS(2-Thiobarbituric Acid Reactive Substances) (Sinnhuber and Yu, 977) g antioxidant(54% propyleneglycol + 40% Tween % BHT + 3% BHA) 3, TBA solution(% TBA + 0.3% NaOH) 3 ml 5. TCA solution(2.5% TCA mm HCl) 7 ml, 00 water bath(ob-25e, Jeio Tech, Korea) ml 0 ml glass tube, chloroform 3 ml 4 3,500 rpm 30 (CS-6R Centrifuge, Beckman Instruments Inc., USA), 3 ml 4.5 ml 532 nm (UC-mini-240, Shimadzu, Japan). kg mg MA(malonaldehyde), blank. (Volatile Basic Nitrogen; VBN) 3 2, Conway (Kohsaka, 975).. 5 g 5% TCA 20 ml Homogenizer(Ultra Turrax T25 basic, Ika Werke Gmbh & Co., Germany) 3,500 rpm 60. Filter paper No.(Whatman International Ltd, UK). Conway s borate butter ml Conway dish, 50% K 2 CO 3 ml K 2 CO , 0.02N H 2 SO 4 mg%. (206) g 0.% peptone 90 ml (Whirl-pak blender bag, Nasco International, USA), stomacher(laboratoryblender400, Seward, England) normal speed. 0 ml petri dish 45 (Plate count Agar, Difco, USA) 5 ml, ±2.. (206) 3 2. ml petri dish 50 VRBA(Violet Red Bile Agar, Difco, USA) 5 ml, petri dish ±2.. 0, (color), (taste), (aroma), (salinity), (overall acceptability). 2. 9,., (Intensity). (MS23F30- TAW, 00W, Samsung, Gyeonggi, Korea),. Agricolae(R-version ) ANOVA (The R-foundation for Statistical Computing, Vienna, Austria), Duncan s multiple range test 5%.

5 Jeong et al. : Effect of Liquid Smoke and Curing Mixture on Quality of Chuncheon Dakgalbi 33 ph Table 3. ph Control ph 6.06 (P<0.05), ph.,,. Table 4. (Sensor sensitivity), Table 3. Instrumental color and ph values of Chuncheon Dakgalbi made with different amounts of liquid smoke Parameters Control S-0.05 S-0.0 S-0.20 ph 6.06±0.0 b 6.2±0.06 a 6.±0.02 a 6.0±0.0 a CIE L* (Lightness) CIE a* (Redness) CIE b* (Yellowness) 46.± ± ± ± ± ± ± ± ± ± ± ±4.27 Control: no liquid smoke, S-0.05: added 0.05% liquid smoke, S-0.0: added 0.0% liquid smoke, S-0.20: added 0.20% liquid smoke. a,b Means in the same row with different letters are significantly different (P<0.05). Table 4. Changes of sensors sensitivity (dr/r0 ) Chuncheon Dakgalbi made with different amounts of liquid smoke Sensor Treatments 2 Number Model 3 Control S-0.05 S-0.0 S-0.20 SY/LG SY/G 0.002± a 0.008± ab ± b ± b 3 SY/AA ± a ± b ± b ± b 4 SY/Gh ± ab ± b 0.004± ab ± a 5 SY/gCT ± ± ± ± SY/gCT 0.003± a ± b ± b ± b 7 T30/ 0.80±0.04 b 0.274± b 0.293± b 0.400± a 8 P0/ ± c ± b 0.07±0.008 b ± a 9 P0/ ± c ± b ±0.008 b ± a 0 P40/ ± c ± b ± b ± a T70/ ±0.008 c ± b ±0.006 b ± a 2 PA2 0.68±0.059 c 0.770± bc 0.820± b ± a All values are mean±s.d. (n=4). dr/r0: Value of the sensors in relative resistance change. dr/ R0 = (R0 R)/ R0. R0: resistance at t = 0 (baseline resistance). R: resistance at selected time. 2 Control: no liquid smoke, S-0.05: added 0.05% liquid smoke, S-0.0: added 0.0% liquid smoke, S-0.20: added 0.20% liquid smoke. 3 SY: a sensor which incorporates a thick film material deposited on interdigitated electrodes on a alumina substrate with the platinum alloy thick heater, T: a conventional sensor using a high quality alumina tube gold printed electrodes connecting gold alloy wires and reliable iron chrome alloy heater, P: a plane type of sensors using thick film technology on a small and thin alumina substrate. a~c Means in the same row with different letters are significantly different (P<0.05).

6 34 :. 7 2 (P<0.05), 2, 3, 6.,. Fig.. (discrimination index),, (Anonymous, 2002). 78, Control,.., Kim et al.(2004),,. Table 5.,,,,, (P<0.05).,. 0.0%. Table 6.,,,. 4 Fig.. Principal component analysis (PCA) of aroma pattern of Chuncheon Dakgalbi with different amounts of liquid smoke. Control: no liquid smoke, S-0.05: added 0.05% liquid smoke, S-0.0: added 0.0% liquid smoke, S-0.20: added 0.20% liquid smoke. Table 5. Consumer preference test of Chuncheon Dakgalbi made with different amounts of liquid smoke Parameters Control S-0.05 S-0.0 S-0.20 Color 6.2±.8 6.5± ± ±.55 Taste 6.7± ± ± ±0.74 Aroma 6.2± ± ± ±.35 Salinity 6.7± ±.29 6.± ±.06 Overall acceptability 6.5±0.53 c 6.8±0.79 bc 7.7±0.48 a 7.3±0.47 ab Control: no liquid smoke, S-0.05: added 0.05% liquid smoke, S-0.0: added 0.0% liquid smoke, S-0.20: added 0.20% liquid smoke. a~c Means in the same row with different letters are significantly different (P<0.05). 33 SC-0.2 SC-0.3 (P<0.05). Muhlisin et al.(202),. ph Table 7. Control, S, SC-0.2 SC-0.3 ph 6.0, 6.06, ph (P<0.05).

7 Jeong et al. : Effect of Liquid Smoke and Curing Mixture on Quality of Chuncheon Dakgalbi 35 Table 6. Instrumental color of Chuncheon Dakgalbi made with different amounts of liquid smoke and curing mixture during storage at 8 Parameters CIE L* (Lightness) CIE a* (Redness) CIE b* (Yellowness) Storage time (day) Control 4.3±0.58 BC 43.8±.07 A 43.4±0.42 ba 43.4±.04 A 40.6±2.46 bc 40.4±0.52 cc 42.6±0.85 bab 43.9±0.67 A S 42.6±0.3 A 44.0±0.64 A 42.6±0.30 ca 4.5±.22 A 42.8±0.23 ab 42.6±0.6 ab 40.5±0.30 ca 43.±0.62 A SC ±.27 C 43.6±0.95 B 42.7±0.28 cc 43.6±0.80 B 44.6±0.89 aa 4.9±0.0 bc 43.7±0.66 ab 44.±0.20 AB SC ±0.44 C 43.2±0.54 ABC 44.9±0.77 aa 42.±2.86 BC 44.±.26 aab 42.6±0.58 abc 42.4±0.23 bbc 43.6±.45 ABC Control 2.8±0.80 bb 8.9±.86 bc 9.3±2.27 bc 22.5±.57 bb 2.2±0.66 cb 2.4 ±0.30 cb 25.6±0.80 ba 26.0±0.48 aa S 22.5±0.72 abb 2.2±.03 ad 22.3±0.64 abc 20.9±0.85 cb 23.9±0.6 bd 9.3±0.08 dcd 22.8±0.5 ca 24.9±0.20 ba SC ±.45 ababc 22.2±0.4 acd 24.0±3.8 aabc 25.7±0.39 aa 24.6±0.08 abab 23.5±0.08 bbcd 2.7±0.39 dd 24.9±0.6 bab SC ±.0 bc 2.6±.38 ad 24.±.6 ac 26.2±0.66 aab 25.3±0.89 abc 27.4±0.27 aa 26.3±0.49 aab 24.2±0.58 cc Control 27.7±0.76 bc 2.0±2.89 be 23.6±.47 bd 27.3±.66 bcc 27.7±2.69 bc 24.4±0.40 bd 34.6 ±0.69 aa 3.0±0.84 ab S 30.4±.34 abb 23.3±.56 abd 24.±0.5 bd 25.8±.39 cc 27.±0.37 bcc 2.4±0. dd 3.2±0.46 ba 29.3±0.40 bb SC ±2.45 aa 25.±0.80 ac 24.4±.39 bc 28.0±0.48 bb 25.5±0.0 cc 22.±0.4 cd 25.5±0.80 cc 28.9±0.65 bb SC ±.22 ababc 23.0±.7 abd 29.5±3.08 abc 3.9±.24 aab 30.3±0.37 aabc 3.5±0.27 aab 32.0±0.8 ba 28.9±0.45 bc Control: no additive, S: added 0.0% liquid smoke, SC-0.2: added 0.0% liquid smoke and 0.20% curing mixture, SC-0.3: added 0.0% liquid smoke and 0.30% curing mixture. A~E Means in the same row with different letters are significantly different (P<0.05). a d Means in the same column with different letters are significantly different (P<0.05). Table 7. ph values of Chuncheon Dakgalbi made with different amounts of liquid smoke and curing mixture during storage at 8 Storage time (day) Control 6.0±0.0 ba 6.05±0.03 A 6.±0.0 A 6.0±0.03 A 6.05±0.03 aa 6.03±0.0 A 5.99±0.04 A 4.63±0.26 bb S 6.06±0.0 abc 6.08±0.0 B 6.3±0.0 A 6.06±0.04 BC 5.95±0.0 bd 6.02±0.02 C 6.04±0.03 BC 5.96±0.03 ad SC ±0.0 ac 6.0±0.02 AB 6.±0.02 A 6.05±0.06 BC 6.04±0.03 ac 6.03±0.0 C 5.95±0.02 D 6.02±0.0 ac SC ±0.0 ab 6.09±0.02 AB 6.3±0.02 A 6.06±0.02 B 6.05±0.0 abc 6.0±0.0 CD 6.0±0.0 CD 5.99±0.06 ad Control: no additive, S: added 0.0% liquid smoke, SC-0.2: added 0.0% liquid smoke and 0.20% curing mixture, SC-0.3: added 0.0% liquid smoke and 0.30% curing mixture. A~D Means in the same row with different letters are significantly different (P<0.05). a~b Means in the same column with different letters are significantly different (P<0.05). ph. ph., 56 Control ph 4.63 S, SC-0.2 SC-0.3 ph (P<0.05). Nychas et al.(998) ph. Makela et al.(992), Korkeala and Makela (989), Samelis et al.(2000), Von et al.(99). ph

8 36 :. TBARS Table 8. TBARS SC mg/kg (P<0.05). TBARS, Control 33, 47, S 47, 56 TBARS, SC-0.2 SC-0.3 TBARS (P<0.05). Muhlisin et al.(202),, TBARS,. Kim et al.(998). Jeong and Shim (2002), Kim et al.(998). VBN Table 9. VBN mg%.,. 47 Control, 56 Control 25.8 mg% S, SC-0.2 Table 8. Thiobarbituric acid reactive substances (TBARS) values (mg malondialdehyde/kg ) of Chuncheon Dakgalbi made with different amounts of liquid smoke and curing mixture during storage at 8 Storage time (day) Control 0.45±0.04 ae 0.56±0.04 E 0.8±0. ad 0.9±0.05 ac 0.92±0.08 C.8±0.06 aa.07±0.04 abb 0.97±0.0 cc S 0.42±0.0 abf 0.55±0.07 E 0.63±0.07 cde 0.70±0.0 cd 0.88±0.0 C.03±0.07 bb.6±0.05 aa.2±0.03 ab SC ±0.05 ac 0.55±0.05 C 0.74±0.09 bb 0.83±0.04 bb 0.94±0.03 A.03±0.2 ba 0.96±0.03 ca.03±0.03 ba SC ±0.03 be 0.46±0.3 D 0.73±0.02 bc 0.82±0.05 bb 0.85±0.05 B.00±0.04 ba.03±0.07 bca.04±0.02 ba Control: no additive, S: added 0.0% liquid smoke, SC-0.2: added 0.0% liquid smoke and 0.20% curing mixture, SC-0.3: added 0.0% liquid smoke and 0.30% curing mixture. A F Means in the same row with different letters are significantly different (P<0.05). a c Means in the same column with different letters are significantly different (P<0.05). Table 9. Volatile basic nitrogen (VBN) contents (mg/00g) of Chuncheon Dakgalbi made with different amounts of liquid smoke and curing mixture during storage at 8 Storage time(day) Control 6.3±0.8 D 5.2±0.40 bd 7.9±.07 C 8.4±0.40 ac 8.4±.07 C 9.5±0.40 C 23.5±.40 ab 25.8±.07 aa S 6.8±0.00 D 7.5±0.00 acd 8.4±0.40 BC 8.2±0.70 abbc 8.4±0.40 BC 8.8±0.40 B 22.3±0.40 aba 22.5±.07 ba SC ±0.99 CD 4.9±0.8 D 7.5±0.70 BC 7.0±0.40 bcc 7.5±.40 BC 9.3±0.00 AB 2.±.46 bca 2.±.07 ba SC ±0.49 DE 5.4±.40 bf 7.5±0.00 CDE 6.6±.07 cef 8.2±0.70 CD 8.8±0.8 BC 20.0±0.70 cb 2.6±0.40 ba Control: no additive, S: added 0.0% liquid smoke, SC-0.2: added 0.0% liquid smoke and 0.20% curing mixture, SC-0.3: added 0.0% liquid smoke and 0.30% curing mixture. A F Means in the same row with different letters are significantly different (P<0.05). a c Means in the same column with different letters are significantly different (P<0.05).

9 Jeong et al. : Effect of Liquid Smoke and Curing Mixture on Quality of Chuncheon Dakgalbi 37 SC-0.3 VBN mg%. Leroi et al.(200),, VBN, VBN. Lund et al.(20),, Byun et al.(2003),,, VBN VBN., Choi et al.(20), ph, TBA VBN. VBN, ph. VBN. Table 0. Control, S, SC-0.2 SC log CFU/g, 3.82 log CFU/g, 3.95 log CFU/g 3.65 log CFU/g, SC-0.2 SC-0.3 (P<0.05)., 56 Control 7.9 log CFU/g, S, SC-0.2 SC log CFU/g, 3.97 log CFU/g 3.69 log CFU/g., Control ph. 28, 33, 56 Control 3.96 log CFU/g,.54 log CFU/g 6.00 log CFU/g. Seo et al.(2000), Noh et al.(2008) aldehyde, phenol, alcohol. Kim et al.(998), ph. Table 0. Aerobic plate count (APC) and total coliforms (TC) of Chuncheon Dakgalbi made with different amounts of liquid smoke and curing mixture during storage at 8 Parameters Storage time(day) Aerobic plate count (CFU/g) Total coliforms (CFU/g) Control 4.±0.07 abc 4.45±0.05 ab 4.38±0.06 ab 4.47±0.08 abb 3.85±0.50 C 4.30±0.06 ab 4.7±0.2 bbc 7.9±0.0 aa S 3.82±0.09 bd 4.4±0.3 bc 4.24±0.05 abc 4.55±0.08 aa 4.4±0.20 C 4.27±0.7 abc 4.50±0.2 aab 4.39±0.0 bab SC ±0.06 bc 4.9±0.02 bab 4.00±0.04 bc 4.33±0.8 ba 4.07±0. BC 3.54±0.09 bd 4.20±0.08 bab 3.97±0.05 cc SC ±0. cb 3.99±0.6 ba 3.50±0.28 cbc 4.00±0.00 ca 3.65±0.4 B 3.30±0.43 bc 3.45±0.05 cbc 3.69±0.09 dab Control 2 ND ND ND ND 3.96±0.9 B.54±0.09 C ND 6.00±0.07 A S ND ND ND ND ND ND ND ND SC-0.2 ND ND ND ND ND ND ND ND SC-0.3 ND ND ND ND ND ND ND ND Control: no additive, S: added 0.0% liquid smoke, SC-0.2: added 0.0% liquid smoke and 0.20% curing mixture, SC-0.3: added 0.0% liquid smoke and 0.30% curing mixture. A D Means in the same row with different letters are significantly different (P<0.05). a c Means in the same column with different letters are significantly different (P<0.05). 2 ND: not detected.

10 38 :. Table.., Control. Control, SC-0.3 < S < SC-0.2, Control < SC-0.2 < S, SC-0.3 (P<0.05). Control, S, SC-0.3 < SC-0.2 SC-0.2 (P<0.05). Massimiliano et al.(203). McGee et al.(2003),., 0.% 0.3% SC-0.3, 0.% 0.2% SC-0.2. Table. Consumer preference test of Chuncheon Dakgalbi made with different amounts of liquid smoke and curing mixture Parameters Control S SC-0.2 SC-0.3 Color 7.7± ± ± ±2.6 Taste 6.2±.69 b 6.5±.43 ab 7.7±0.82 a 5.5±.78 b Aroma 5.8± ±.0 6.8± ±.58 Salinity 4.0±.49 b 5.8±.75 a 5.3±.34 ab 6.3±.64 a Overall acceptability 6.4±.07 b 6.7±.4 b 7.9±0.99 a 6.3±.49 b Control: no additive, S: added 0.0% liquid smoke, SC-0.2: added 0.0% liquid smoke and 0.20% curing mixture, SC-0.3: added 0.0% liquid smoke and 0.30% curing mixture. a,b Means in the same row with different letters are significantly different (P<0.05). (0%, 0.05%, 0.%, 0.2%) 0.% (0.2%, 0.3%). ph,,., 0.%.., ph, TBARS VBN,, 0.3%.,., 0.3% 0.2%. 0.% 0.2%. 206 (C ). Anonymous 200 Semi-Conduct Sensors. Alpha MOS, Toulouse, France. Anonymous 2002 Operating Manual. Alpha MOS, Toulouse, France. Byun JS, Min JS, Kim IS, Kim JW, Chung MS, Lee MH 2003 Comparison of indicators of microbial quality of meat during aerobic cold storage. J Food Prot 66(9):

11 Jeong et al. : Effect of Liquid Smoke and Curing Mixture on Quality of Chuncheon Dakgalbi 39 Choi YS, Choi JH, Kim HY, Kim HW, Lee MA, Chung HJ, Lee SK, Kim CJ 20 Effect of Lotus (Nelumbo nucifera) leaf powder on the quality characteristics of chicken patties in refrigerated storage. Korean J Food Sci An 3():9-8. Choi YI, Kim BC, Park GB, Sung SK, Lee MH, Lee SK, Chung MS, Joo ST, Choi YI 998 Processing of red meat. Pages In: The Science of Muscle Foods. Sunjin munhwasa, Gyeonggi, Korea. Gheisari HR, Hamzeloo A, Hosseinzadeh S, Basiri S 207 Curing effects on the growth of Listeria monocytogenes and Escherichia coli O57:H7 in camel meat using most probable number-polymerase chain reaction method. J Microbiol Biotech Food Sci 6(4): Jeong CH, Shim KH 2002 Nitrite-scavenging and antioxidant activities of wood vinegar. Korean J Food Preserv 9(3): Kim YJ, Kim MB, Moon YI, Kim YB 99 Processing of brine soaked and smoked duck meat: pan-broiling yield and sensory evaluation of brined or smoked duck meat. Korean J Ani Sci Technol 33: Kim JY, Kim YS, Lee SK 2004 Aroma pattern analysis of hanwoo beef (M. longissimus) using electronic nose during refrigerated storage. Korean J Food Sci An 24(3): Kohsaka K 975 Freshness preservation of food and measurement. The Food Industry 8:05-. Korkeala H, Makela P 989 Characterization of lactic acid bacteria isolated from vacuum-packed cooked ring sausages. International J Food Microbiol 9: Lee KI, Hwang YJ, Ban HJ, Lim SJ, Jin HJ, Lee HS 205 Impact of the growth of single-person households on the food market and policy tasks. Pages 2-45 In: Korea Rural Economic Institute. Jeollanam-do, Korea. Leroi F, Joffraud JJ, Chevalier F, Cardinal M 200 Research of quality indices for cold-smoked salmon using a stepwise multiple regression of microbiological counts and physico-chemical parameters. J Appl Microbiol 90(4): Lund MN, Heinonen M, Baron CP, Estevez M 20 Protein oxidation in muscle foods: A review. Mol Nutr food Res 55(): Makela P, Schillinger U, Korkeala H, Holzapfel WH 992 Classification of ropy slime-producing lactic acid bacteria based on DNA-DNA homology, and identification on Lactobacillus sake and Leuconostoc amelibiosum as dominant spoilage organisms in meat products. International J Food Microbiol 6: McGeea MR, Henryb KL, Brooksb JC, Rayb FK, Morganb JB 2003 Injection of sodium chloride, sodium tripolyphosphate, and sodium lactate improves Warner-Bratzler shear and sensory characteristics of pre-cooked inside round roasts. Meat Sci 64(3): Ministry of Food and Drug Safety 206 Processing of Standards and Ingredients Specifications for Livestock Products. Osong, Korea. Pages Muhlisin, Kang SM, Choi WH, Kim CJ, An BK, Kang CW, Lee SK 202 New approach to Chuncheon Dakgalbi processing by various chicken materials, seasoning and cooking methods. Korean J Food Sci An 32(6): Noh BS, Kim SS, Jang PS, Lee HG, Kim TJ 2008 Food Preservation. Soohaksa, Korea. Pages Nychas GJE, Drosinos EH, Board RG 998 Chemical changes in stored meat. Pages In: The Microbiology of Meat and Poultry, Davies A, Blackie Academic and Professional. London, UK. Samelis J, Kakouri A, Rementzis J 2000 The spoilage microflora of cured, cooked turkey breasts prepared commercially with or without smoking. International J Food Microbiol 56: Seo KI, Ha KJ, Bae YI, Jang JK, Shim KH 2000 Antimicrobial activity of oak smoke flavoring. Korean J Posthavest Sci Technol 7(3): Sinnhuber RO, Yu TC 977 The 2-thiobarbituric acid reaction, an objective measure of the oxidative deterioration occurring in fats and oils. J Japan Soc Fish Sci 26: Von Holy A, Cloete TE, Holzapfel WH 99 Quantification and characterization of microbial populations associated with spoiled, vacuum-packed Vienna sausages. Food Microbiol 8(2): Received Dec. 6, 207, Revised Mar. 26, 208, Accepted Mar. 27, 208

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