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Korean Journal of Environmental Agriculture Korean J Environ Agric. 2016;35(3):184-190. Korean Online ISSN: 2233-4173 Published online 2016 September 30. http://dx.doi.org/10.5338/kjea.2016.35.3.25 Print ISSN: 1225-3537 Research Article Open Access 블루베리 (Vaccinium corymbosum L.

1 Korean Journal of Environmental Agriculture Korean J Environ Agric. 2016;35(3): Korean Online ISSN: Published online 2016 September Print ISSN: Research Article Open Access 블루베리 (Vaccinium corymbosum L.) 품종별안토시아닌조성및함량 이민기 1, 김헌웅 1, 이선혜 1, 김영진 1, 장환희 1, 정현아 2, 황유진 1, 최정숙 1, 김정봉 1* 1 국립농업과학원농식품자원부, 2 전북대학교생활과학대학식품영양학과 Compositions and Contents Anthocyanins in Blueberry (Vaccinium corymbosum L.) Varieties Min-Ki Lee 1, Heon-Woong Kim 1, Seon-Hye Lee 1, Young Jin Kim 1, Hwan-Hee Jang 1, Hyun-Ah Jung 2, Yu-Jin Hwang 1, Jeong-Sook Choe 1 and Jung-Bong Kim 1* ( 1 Department of Agro-food Resources, National Institute of Agricultural Sciences, Wanju, 55365, Korea, 2 Department of Food Science and Human Nutrition, College of Human Ecology, Chonbuk National University, Jeonju , Korea) Received: 20 July 2016 / Revised: 12 September 2016 / Accepted: 26 September 2016 Copyright c 2016 The Korean Society of Environmental Agriculture This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ORCID Min-Ki Lee Jung-Bong Kim Abstract BACKGROUND: Anthocyanins, potential health-promoting compounds, were major natural pigment in the blueberry (Vaccinium corymbosum L.). The objectives of this study was to investigate anthocyanin glycosides in the blueberry varieties. METHODS AND RESULTS: A total of seventeen anthocyanins were identified from highbush blueberry using HPLC (representatives, 530 nm) and ESI-MS in positive ion mode. The individual anthocyanins are containing cyanidin, delphinidin, malvidin, peonidin, and petunidin moieties which are acylated with aliphatic acid (acetic acid) and conjugated with sugar moieties (arabinose, galactose, and glucose). Among them, delphinidin 3-Ogalactoside (D3Ga), peonidin 3-O-glucoside (Pn3G) + malvidin 3-O-galactoside (M3Ga) were major compounds in varieties. Total anthocyanins were found the highest level in 'Elizabeth' (1,406.3 mg/100 g dry weight) which *Corresponding author: Jung-Bong Kim Phone: ; Fax: ; jungbkim@korea.kr was 3-fold higher than Darrow (465.7). Especially, D3Ga was presented the 32% of total anthocyanins followed by Pn3G + M3Ga (20%) in Elizabeth. CONCLUSION: This result was showed as valuable information regarding nutritional properties of the different varieties of the highbush blueberry. Key words: Anthocyanins, Delphinidin glycosides, Malvidin glycosides, Vaccinium corymbosum L. 서론 블루베리 (Vaccinium corymbosum L.) 는빌베리 (bilberry), 크렌베리 (cranberry) 와함께산앵두나무속에속하는식물이다. 수고에따라하이부쉬 (V. corymbosum L.), 로우부쉬 (V. angustifolium L.), 반수고블루베리그리고레빗아이 (V. ashei L.) 블루베리로분류되고휴면기간에따라북부형과남부형으로나뉘며, 국내에는주로하이부쉬블루베리가재배되고있다 (Kim et al., 2010; Kim et al., 2011). 블루베리는식물의이차대사산물로알려진안토시아닌, 플라보노이드, 각종페놀화합물등을기능성성분을함유하고있다 (Bang et al., 2010; Cardenosa et al., 2016). 안토시아닌 184

2 Anthocyanins in Blueberry Varieties 185 Fig. 1. Chemical structure of six aglycones of anthocyanins. 은천연식물색소로서 6종으로나뉘며 (Fig. 1), glucose, rhamnose 등의당과아실화되어구조적형태에따라약 600여종이존재한다고알려져있다 (Wu and Prior, 2005). 블루베리는과실자체또는가공형태에따라자유라디칼, 활성산소에의한산화적스트레스억제, 저혈당, 항암, 심혈관질환등다양한기능성이알려져있어주스, 와인, 잼등의가공식품으로이용되고있다 (Cho et al., Su and Silva, 2006; Neto, 2007; Rossi et al., 2003). 최근블루베리내안토시아닌에관한연구에의하면블루베리로부터 cyanidin, delphinidin, malvidin, peonidin, petunidin 배당체등 13종의안토시아닌개별성분을분리, 동정하고숙기에따른품종별항산화활성을조사하였고 (Castrejon et al., 2008), 블루베리와빌베리로부터 15종의안토시아닌을분리하고 malvidin 배당체 (galactose, glucose, arabinose) 가블루베리의주요성분이었다 (Bornsek et al., 2012). 또한블루베리는주스의가공과정중안토시아닌함량변화뿐만아니라수확후저장온도에따른안토시아닌함량변화등다양한분야에서연구되고있다 (Kalt et al., 1999; Skrede et al., 2000). 국내에서는블루베리에대한과실의수량과품질등의형태적특성과품종및가공처리에따라조추출물의형태로항산화효과를측정하는연구가많은비중을차지하고있다 (Jeong et al., 2012, Kim et al., 2015). 몇몇연구에서대사체학을기반으로블루베리안토시아닌및페놀화합물을포함하는대사체분석하였으나국내산블루베리안토시아닌개별성분에관한연구는미흡한실정이다 (Jo et al., 2014). 이와같이블루베리는안토시아닌등체내에서생리활성을갖는이차대사산물이풍부한소재로각광받아활발히연구되고있다. 따라서본연구는하이부쉬블루베리내안토시아닌개별성분을분석하고품종에따른함량차이를조사함으로서안토시아닌고함유식품소재로활용가능성을재고하고이를위하여필요한개별성분정보를제공하고자하였다. 재료및방법 시약및기기 Acetonitrile, water는 Fisher Scientific( 미국 ) 사로부터구입하여사용하였으며, formic acid는 Junsei Chemical( 일본 ) 사제품을사용하였다. 내부표준품으로사용한 cyanidin-3, 5-di-O-glucoside(cyanin) 는 Extrasynthese( 프랑스 ) 로부터구입하여사용하였다. 정성및정량을위해 photodiode array detector(2998, Waters Co., Milford, Massachusetts, USA) 가장착된 HPLC system(alliance e2695, Waters Co., Milford, Massachusetts, USA) 및 MS(Mircomass ZQ, Waters Co., Milford, Massachusetts, USA) 를사용하였다. 시료전처리본연구에사용된하이부쉬블루베리는총 8품종으로충남농업기술원으로부터제공받은시료를사용하였다. 건조분말시료 2 g을 conical tube(50 ml) 에담아 20 ml의 5% formic acid in water를넣어 40 진탕항온수조에서 24시간추출한후, 원심분리 (3,000 rpm, 10분, 4 ) 하여상층액 1 ml 를취하였다. Sep-pak C18 classic cartridge 를 MeOH 2 ml, water 2 ml의순으로흘려주어활성화시킨다음, 안토시아닌추출상층액 1 ml 및내부표준물질 (cyanin, 100 ppm) 1 ml을각각 loading 후, water 2 ml로세척하여 MeOH 1 ml로용출시켰다. 용출된안토시아닌여과액은 N 2 가스로농축한후, 1 ml의 5% formic acid in water로재용해하여 LC-PDA-ESI/MS로분석하였다. 각개별성분은내부표준물질 (cyanin) 과비교하여상대적인값을나타내었고, 품종별로 3회반복시행하여얻어진결과를평균값으로표시하였다. LC-PDA-ESI/MS 를이용한안토시아닌개별성분분석안토시아닌개별성분을정성및정량하기위하여 Synergi

3 186 Lee et al. Polar-RP 80A( mm I.D., 4 μm; Phenomenex, USA) 를장착한 LC-PDA-ESI/MS를사용하였다. 검출파장은 250~600 nm( 대표파장 530 nm) 로설정하였고, 시료주입량은 10 μl, 컬럼오븐온도는 30, 유속은 1 ml/min 로설정하였다. 이동상은 A(5% formic acid in water) 와 B(5% formic acid in water/acetonitrile, 1:1, v/v) 를사용하였다. 각성분을분리하기위하여다음과같은용매구배조건으로수행되었다. 30분동안 solvent B의농도를 20에서 50% 로일정하게증가시켰고, 5분동안 50% 를유지시킨후, 다시 5분동안 50에서 20% 의농도로일정하게감소시킨다음, 10분간 20% 를유지하여안정화시켰다. MS 분석은 electrospray ionization(esi) source를이용한 positive ionization mode로분석되었으며, MS parameter로각각 cone voltage 30 V, source 온도 120, desolvation 온도 500, desolvation N 2 가스 1,020 L/hr 로설정하였다. 분자량범위는 full scan 타입으로 m/z 로설정하였다. 결과및고찰 하이부쉬블루베리품종별안토시아닌분석하이부쉬 (highbush) 블루베리 8 품종 ( Bluecrop, Bluegold, Chandler, Darrow, Elizabeth, Legacy, Nelson, Patriot ) 에함유된안토시아닌분석은 PDA가장착된 HPLC로대표파장 530 nm에서분석하였고 electrospray ionizion source가장착된 single quadrupole MS에서 positive ion mode로분석하였다. 하이부쉬블루베리로부터총 17종의안토시아닌개별성분을확인하였으며, 각피크의단편이온의패턴, 머무름시간을분석하여안토시아닌의조성을확인하였다 (Fig. 2). 분리된안토시아닌은 cyanidin, delphinidin, malvidin, peonidin, petunidin 을기본구조로하여 3번탄소에 1개의당 (galactose, glucose, arabinose) 과당에 acetic acid가아실화되어있는구조를나타내었다 (Table 1). 각성분에서 1개의당분자가잘려나가는단편이 Fig. 2. LC chromatogram of anthocyanins using high-performance liquidchromatography-photodiode array detector (530 nm) coupled to electrospray ionization mass spectrometry form the highbush blueberry ( Bluegold ). Table 1. Identified seventeen anthocyanins and their mass spectrometric data from the highbush blueberry (Vaccinium corymbosum L.) No. Chemical names Abbreviations MW Fragment ion patterns (m/z) 1 Delphinidin 3-O-galactoside D3Ga /303 2 Delphinidin 3-O-glucoside D3G /303 3 Cyanidin 3-O-galactoside C3Ga /287 4 Delphinidin 3-O-arabinoside D3A /303 5 Cyanidin 3-O-glucoside C3G /287 6 Petunidin 3-O-galactoside Pt3Ga /317 7 Petunidin 3-O-glucoside Pt3G /317 8 Cyanidin 3-O-arabinoside C3A /287 9 Peonidin 3-O-galactoside Pn3Ga / Petunidin 3-O-arabinoside Pt3A / Peonidin 3-O-glucoside Pn3G / Malvidin 3-O-galactoside M3Ga / Malvidin 3-O-glucoside M3G / Malvidin 3-O-arabinoside M3A / Petunidin 3-O-(6"-O-acetoyl)glucoside Pt3,6AcG / Malvidin 3-O-(6"-O-acetoyl)galactoside M3,6AcGa / Malvidin 3-O-(6"-O-acetoyl)glucoside M3,6AcG /331 * All samples analyzed in positive ion mode (m/z, [M] + ) using LC-PDA-ESI/MS * No., the elution order of anthocyanins from HPLC chromatogram.

4 Anthocyanins in Blueberry Varieties 187 Fig. 3. Electrospray ionization mass spectrometry (ESI-MS) spectra (positive ion mode, [M] + ) of anthocyanins identified from the highbush blueberry (Vaccinium corymbosum L.). a), delphinidin 3-O-glalactoside; b), delphinidin 3-O-glucoside; c), cyanidin 3-O-galactoside + delphinidin 3-O-arabinoside; d), cyanidin 3-O-glucoside; e), petunidin 3-O-galactoside; f), petunidin 3-O-glucoside; g), cyanidin 3-O-arabinoside; h), peonidin 3-O-galactoside + petunidin 3-O-arabinoside; i), peonidin 3-O-glucoside + malvidin 3-O-galactoside; j), malvidin 3-O-glucoside; k), malvidin 3-O-arabinoside; l), petunidin 3-O-(6"-O-acetyl)glucoside; m), malvidin 3-O-(6"-O-acetyl)galactoside; n), malvidin 3-O-(6"-O-acetyl)glucoside. 온패턴을나타내었으며 (Fig. 3), 동일한단편이온패턴을갖는성분은타연구결과를바탕으로안토시아닌개별성분을확인하였다 (Cardenosa et al., 2016; Nakajima et al., 2004). 동일한 aglycone 을갖는안토시아닌의경우당종류에따라머무름시간의차이가있는것을알수있었고 galactose glucose arabinose 의순으로검출되는것을확인하였다. Malvidin과 petunidin에서는아실화되어있는형태도확인되었으며결합된당 (galactose와 glucose) 의 6" 번위치에 acetic acid가결합되어있는것을확인하였다. Cyanidin 3-O- galactoside(c3ga) 와 delphinidin 3-O-arabinoside(D3A), cyanidin 3-O-glucoside(C3G) 와 petunidin 3-O-galactoside (Pt3Ga), peonidin 3-O-galactoside(Pn3Ga) 와 petunidin 3-O-arabinoside(Pt3A), peonidin 3-O-glucoside(Pn3G) 와 malvidin 3-O-galactoside(M3Ga) 는본연구에사용된용매구배조건에서는분리되지않았다. 하이부쉬블루베리품종별안토시아닌상대적함량변화본연구에사용된블루베리중남부형인 Legacy 를제외

5 188 Lee et al. Table 2. Relative contents of anthocyanins (mg/g dry weight) in different highbush blueberry (Vaccinium corymbosum L.) varieties Variety Harvest season D3Ga D3G C3Ga + D3A C3G + Pt3Ga Pt3G C3A Pn3Ga + Pn3A Chemical names Pn3G + M3Ga M3G M3A Pt3,6AcG M3,6AcGa M3,6AcG Total Patriot Early-season Bluegold Early-season Legacy Mid-season NDa) ND ND Bluecrop Mid-season ND ND Elizabeth Mid-season Darrow Late-season Nelson Late-season Chandler Late-season ND ND a) ND, not detected. 한 7 품종이북부형하이부쉬블루베리였으며, Patriot, Bluegold 는조생종, Legacy, Bluecrop, Elizabeth 는중생종이었고 Chandler, Darrow, Nelson 은만생종이었다. 8 품종의하이부쉬블루베리에함유되어있는총안토시아닌의함량범위는 465.7~ mg/100 g dry weight(dw) 로나타났다 (Table 2). 중생종의총안토시아닌함량은평균 1,385.6 mg/100 g DW로가장높았고조생종 (1,214.2), 만생종 (642.6) 의순으로숙기에따라총안토시아닌함량변화를보였다. 품종별총안토시아닌함량은 Elizabeth (1,406.3) > Legacy (1,382.4) > Bluecrop (1,368.1 mg/100 g DW) 순으로중생종이높았으며, Elizabeth 는만생종인 Darrow (465.7 mg/100 g DW) 보다약 3배높았다. 블루베리는같은산앵두나무속에속하는빌베리 (V. myrtillus) 의안토시아닌조성과비슷한경향을나타내었으며, 총안토시아닌함량은빌베리 (599.7 mg/100 g fresh weight, FW) 와비슷하였고 blackcurrant (235.6) cowberry(68.2) 보다높았다 (Kahkonen et al., 2003). 조생종의경우, 총안토시아닌함량은 Bluegold (1,299.9) 가 Patriot (1,128.5 mg/100 g DW) 보다약간높았고조생종내에서큰차이는없었다. Patriot 에서는총 malvidin 배당체 (Pn3G 포함 ) 함량비율이약 40% 로 delphinidin 배당체 (C3Ga 포함, 약 31%) 보다높았고 malvidin 3-Oglucoside(M3G) 가 mg/100 g DW로가장높은성분으로나타났다. 블루베리의주요안토시아닌계열로 malvidin 배당체가총함량의약 52%, 다음으로 delphinidin 배당체가약 26% 를차지한다고보고되어진연구결과와비슷한경향을나타내었다 (Bornsek et al., 2012). 반면에 Bluegold 에서는 malvidin, delphinidin 배당체비율이각각약 39, 38% 로비슷하였고 delphinidin 3-O-galactoside(D3Ga, mg/100 g DW) 이주요성분으로나타났다. 중생종과만생종에서는미검출또는검출되지않은아실화된안토시아닌인 petunidin 3-O-(6"-O-acetoyl)glucoside(Pt3,6AcG), malvidin 3-O-(6"-O-acetoyl)galactoside(M3,6AcGa), malvidin 3-O-(6"-O-acetoyl)glucoside(M3,6AcG) 은조생종에서상대적으로높게나타났다. 중생종의경우, 안토시아닌함량이 1,368.1~1,406.3 mg/ 100 g DW이었으며, 만생종 (465.7~755.7) 에비하여평균안토시아닌함량이약 2배더높았다. 중생종 3품종의총 delphinidin 배당체 (C3Ga 포함 ) 함량은평균 mg/100 g DW로총 malvidin 배당체 (Pn3G 포함 ) 함량평균 (447.3) 보다약 1.4배높아주요 aglycone임을알수있었고 D3Ga( 평균 405.2) 가주성분으로나타났다. 타연구의결과와마찬가지로블루베리의안토시아닌함량이품종에따라차이가나타났고배당체중 galactose 결합이 glucose 결합보다더많은비중을차지하였으며, 품종에따라주요 aglycone인 delphinidin, malvidin 배당체들의함량비율이달라지는것을확인하였다. 또한 D3Ga가 Bluecrop 내주요성분으로나타나본연구와비슷한경향을나타내었다 (Cho et al., 2004). 남부형하이부쉬블루베리 ( Legacy ) 와북부형하이부쉬블루베리 ( Bluecrop, Elizabeth ) 간안토시아닌조성과함량은큰차이가없는것으로나타났다. 중생종의 M3G 함량 ( 평균 15.0 mg/100 g DW) 은조생종 ( 평균 mg/100 g DW) 과비교하여약 11배감소하였다. 만생종인 Nelson 과 Chandler 의안토시아닌함량 ( 각각 706.4, mg/100 g DW) 은비슷하였고, Darrow 의안토시아닌함량은 mg/100g로가장낮았다. Nelson 과 Chandler 는 delphinidin 배당체 (C3Ga 포함 ) 함량비율이각각약 39, 42% 로 malvidin 배당체 (Pn3G 포함 ) 보다높았으나, Darrow 에서는 malvidin 배당체 (Pn3G 포함, 약 47%) 가주요안토시아닌으로나타났다. 이와같이 8품종의하이부쉬블루베리의안토시아닌조성및함량을조사한결과, delphinidin 배당체와 malvidin 배당체가총함량의약 70% 이상을차지하고있는주된성분들이었고 D3Ga, Pn3G+M3Ga, M3G가주요성분인것을알수있었다. Delphinidin 배당체와 malvidin 배당체의비율및함량, 총안토시아닌함량은같은숙기와품종에따라다른함량의차이가있는것으로나타났다. 국외에서재배된 87

6 Anthocyanins in Blueberry Varieties 189 종의하이부쉬블루베리의안토시아닌을정량분석한결과, 본연구결과와함량과차이가나타났다 (Ehlenfeldt and Prior, 2001) 이는블루베리내안토시아닌조성과함량의차이가품종을포함한여러가지요인에의해설명될수있음을의미한다. 타연구에의하면빛, 온도, 토양, 재배법등의환경적인요인이이차대사산물의합성에영향을미칠수있다고보고하였으며 (Saure, 1990) 이로인하여안토시아닌합성에영향을미쳤을것으로설명되어질수있다. 최근에는식물의방어물질로서의이차대사산물이다양한기능성을나타낸다는여러연구결과를바탕으로기능성성분의관한연구가활발히이루어지고있다. 블루베리는안토시아닌뿐만아니라플라보노이드 (myricetin, quercetin 배당체등 ) 등다양한이차대사산물을함유하고있는것으로알려져있다 (Miles et al., 2013). 안토시아닌, 플라보노이드, 페놀화합물등블루베리내이차대사산물의생리활성에관한연구를통하여그중요성이점차증가되고있다 (Ehlenfeldt and Prior, 2001; Wang et al., 2012). 이에따라안토시아닌섭취후체내대사과정의이용률을쥐, 사람을대상으로연구되고있다 (Cao et al., 2001; McGhie et al., 2003). 비록섭취한안토시아닌이모두체내에서이용되지는않고체외로배출된다고알려져있지만 (Matsumoto et al., 2001; Wu et al., 2002) 안토시아닌의기능적으로긍정적인효과때문에안토시아닌고함유식품을통한적절한섭취가필요하다고판단된다. 따라서국내산하이부쉬블루베리내안토시아닌개별성분의정확한정성, 정량평가와함께안토시아닌고함유품종을조사함으로서블루베리가안토시아닌고함유소재로활용가능성을제시할수있으며향후안토시아닌개별성분의대사, 생리활성과관련된연구에기초정보를제공할수있을것으로사료된다. 결론 본연구에서는품종및숙기별하이부쉬블루베리내안토시아닌의개별성분분석을통하여안토시아닌고함유자원을조사하였다. 총 8 품종의하이부쉬블루베리로부터총 17 종의안토시아닌이확인되었으며, 총안토시아닌함량범위는품종및숙기에따라차이를보였고중생종의함량이높게나타났다. 안토시아닌고함유품종으로는중생종에속하는 Elizabeth 가 1,406.3 mg/100 g DW로가장높은함량을나타냈다. 본연구결과를토대로국내에서재배된 8종의하이부쉬블루베리내안토시아닌의조성및함량분석을통하여안토시아닌고함유식품소재로활용될가능성이있을것으로판단되며, 향후이와관련된연구에기초정보를제공할수있을것으로사료된다. Acknowledgment This study was carried out with the support of Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ ) Rural Development Administration, Republic of Korea. References Bang, I. S., Yu, C.Y., & Lim, J. D. (2010). Effects of temperature and UV irradiation on stability of anthocyanin-polyphenol copigment complex in mulberry fruits. Korean Journal of Medicinal Crop Science, 18(3), Bornsek, S. M., Ziberna, L., Polak, T., Vanzo, A., Ulrih, N. P., Abram, V., & Passamonti, S. (2012). Bilberry and blueberry anthocyanins act as powerful intracellular antioxidants in mammalian cells. Food Chemistry, 134(4), Cao, G., Muccitelli, H. U., Sanchez-Moreno, C., & Prior, R. L. (2001). Anthocyanins are absorbed in glycated forms in elderly women: a pharmacokinetic study. The American Journal of Clinical Nutrition, 73(5), Cardenosa, V., Girones-Vilaplana, A., Muriel, J. L., Moreno, D. A., & Moreno-Rojas, JM. (2016). Influence of genotype, cultivation system and irrigation regime on antioxidant capacity and selected phenolics of blueberries (Vaccinium corymbosum L.). Food Chemistry, 202, Castrejon, A. D. R., Eichholz, I., Rohn, S., Kroh, L. W., & Huyskens-Keil, S. (2008). Phenolic profile and antioxidant activity of highbush blueberry (Vaccinium corymbosum L.) during fruit maturation and ripening. Food Chemistry, 109(3), Cho, M. J., Howard, L. R., Prior, R. L., & Clark, JR. (2004). Flavonoid glycosides and antioxidant capacity of various blackberry, blueberry and red grape genotypes determined by high-performance liquid chromatography/mass spectrometry. The Journal of the Science of Food and Agriculture, 84(13), Ehlenfeldt, M. K., & Prior, R. L. (2001). Oxygen radical absorbance capacity (ORAC) and phenolic and anthocyanin concentrations in fruit and leaf tissues of highbush blueberry. Journal of Agricultural and Food Chemistry, 49(5), Jeong, H. R., Jo, Y. N., Jeong, J. H., Kim, H. J., & Heo, H. J. (2012). Nutritional composition and in vitro antioxidant activities of blueberry (Vaccinium ashei) leaf. The Korean Society of Food Preservation, 19(4), Jo, Y. H., Kim, S., Kwon, D. A., Lee, H. J., Choi, H. K., & Auh, J. H. (2014). Metabolomic analysis of ethyl acetate and methanol extracts of blueberry. Journal of the Korean Society of Food Science and Nutrition,

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