Effect of Carbon Dioxide Laser on the Clinical Parameters and Crevicular IL-1β When used as an adjunct to Gingival Flap Surgery Kyung-Hee Choi The Gra

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Effect of Carbon Dioxide Laser on the Clinical Parameters and Crevicular IL-1β When used as an adjunct to Gingival Flap Surgery Kyung-Hee Choi The Graduate School Yonsei University Department of Dental Science

Effect of Carbon Dioxide Laser on the Clinical Parameters and Crevicular IL-1β When used as an adjunct to Gingival Flap Surgery A Dissertation Thesis Submitted to the Department of Dental Science and the Graduate School of Yonsei University in partial fulfillment of the requirements for the degree of Master of Dental Science Kyung-Hee Choi June 2003

This certifies that the dissertation thesis of Kyung-Hee Choi is approved. Thesis Supervisor: Chong-Kwan Kim Seong-Ho Choi Yun-Jung Yoo The Graduate School Yonsei University June 2003

Table of Contents Abstract(English) iii. Introduction 1. Materials and Methods 5 A. Clinical sampling and design 5 B. Clinical parameters 7 1. Bleeding on probing 7 2. Probing pocket depth and probing attachment level 7 3. Gingival recession 8 C. Surgical procedure 8 D. Application of Laser 9 E. Collection and assay of IL-1β 10 F. Statistical analysis 11. Results 12 A. Bleeding on probing 12 B. Probing pocket depth 13 C. Clinical attachment level 15 D. Concentration of IL-1β in crevicular fluid 16. Discussion 18. Conclusion 25 References 27 Clinical photos 33 Abstract(Korean) 35 i

List of Tables and Figures Table 1. Flow diagram showing the experimental procedure 6 Table 2. Bleeding on probing 12 Table 3. Probing pocket depth 14 Table 4. Clinical attachment level 15 Table 5. Changes of crevicular IL-1β from baseline to 6 weeks 17 Figure 1. Bleeding on probing 13 Figure 2. Probing pocket depth 14 Figure 3. Clinical attachment level 16 Figure 4. Concentration of IL-1β in crevicular fluid 17 Figure 5-a. A view of presurgery 33 Figure 5-b. A view of debridment 33 Figure 5-c. A view of laser irradiation 33 Figure 5-d. A view of suture 34 Figure 5-e. A view of stitch-out 34 Figure 5-f. A view of 1 month after 34 ii

Abstract Effect of Carbon Dioxide Laser on the Clinical Parameters and Crevicular IL-1When used as an adjunct to Gingival Flap Surgery The objective of the present study was to evaluate the effect of a carbon dioxide (CO 2 ) laser treatment on the clinical parameters and crevicular Interleukin-1(IL-1 ) levels when used in combination with gingival flap surgery. Twelve patients with moderate to advanced periodontitis were selected for this study. Three quadrants of each patient were randomly assigned to one of the following study groups: 1) flap surgery only as the (control); 2) flap surgery and laser treatment using an energy level of 0.8 W as (group 1); 3) flap surgery and laser treatment using an energy level of 0.5 W as (group 2). The gingival crevicular fluid (GCF) was collected at the baseline and biweekly for 6 weeks and the amount of IL-1concentration in sulcular fluid was measured using an enzyme-linked immunosorbent assay (ELISA). The clinical parameters such as the probing pocket depth, the clinical attachment level, the iii

gingival recession and the bleeding on probing were recorded at baseline, 3, 6 months. The results were as follows; marked reductions of the bleeding on probing, the probing pocket depth, the clinical attachment level and a reduction in the crevicular IL-1concentration were found in all groups. However, the differences between the groups in terms of bleeding on probing and the probing pocket depth were not significant (p<0.05). The clinical attachment level and the crevicular IL-1level were significantly lower in group 1 (0.8 W) than in the control (p<0.05). In conclusion, additional use of a Carbon Dioxide laser on the root surface during gingival flap surgery may enhance the clinical attachment and reduce crevicular IL-1concentration. Key words : Carbon dioxide laser; periodontitis; gingival flap surgery; interleukin-1 clinical attachment level iv

Effect of Carbon Dioxide Laser on the Clinical Parameters and Crevicular IL-1When used as an adjunct to Gingival Flap Surgery Kyung-Hee Choi, D.D.S. Department of Dental Science, Graduate School, Yonsei University (Directed by Prof. Chong-Kwan Kim, D.D.S., M.S.D., PhD.). Introduction Removing calcified deposits, microorganisms, microbial products from a diseased root surface is essential in periodontal therapy in order to achieve biologically acceptable root surface (Zander et al., 1976; Patters et al., 1982; O Leary, 1986). Scaling and root planing are widely used for such purposes. It is difficult to completely remove plaque and calculus by scaling and root planing with or without surgical access (Jones and O Leary, 1978; Waerhaug, 1978; Stambaugh et al., 1981; Buchanan and Robertson, 1987; Kepic et al., 1990). The remaining calculus may act as a foundation for bacterial recolonization and the accumulation of metabolic by-product. This type of

microbial growth caused bad result in therapeutic aspect and can be the cause of a recurrence of the disease and a failure of the therapy (Waerhaug, 1978; Patters et al., 1982). Therefore, there needs to be a more effective means of removing residual plaque and calculus. Some reports suggested using lasers in addition to the conventional scaling and root planing (Iwase et al., 1989; Myers, 1991). In theory, it is possible to remove the residual organic debris including microbial plaque and calculus without causing any damage to the adjacent root surfaces with a laser. The most widely used lasers in dentistry are the CO 2 laser, the Nd:YAG laser, the Er:YAG laser and the argon laser. Lasers have several uses to periodontal treatment such as sterilization by the ablation of the dental deposits and root conditioning by the ablation of the diseased root surface. Several researchers have reported the application of laser in periodontal therapy. White et al. (1991) and Cobb et al. (1992) suggested the laser as an adjunctive procedure for periodontal treatment. They reported that a laser could remove calculus and be used in subgingival root planing. In addition, it was reported that the number of periodontal pathogens were reduced and Yamaguchi et al. (1997) reported the elimination of endotoxin.

Besides these effects, it has also been reported that the laser radiation effectively exposes the dentinal tubules by removing the smear layer which is related to the attachment of the connective tissue. Morlock et al. (1992) showed that when a laser was used together with conventional root planing, the smear layer that was observed when root planing alone was performed was eliminated and the dentinal tubules were effectively exposed. Ito et al. (1993) also reported the exposure of the dentinal tubules and collagen fibers. Interleukin-1 (IL-1) is one of the cytokines produced by the activated mononuclear phagocytes derived from the peripheral blood or tissue and the β form has an important catabolic effect on the bone tissue (Grower et al., 1983; Gowen and Mundy, 1986). Some reports have shown that interleukin-1β (IL-1β) plays a crucial role in the destruction of the periodontal tissues (Page, 1991; Stashenko et al., 1991; Jandinski et al, 1991). IL-1β is found in the periodontal tissue and gingival crevicular fluid (GCF) of patients with periodontal disease (Charon et al., 1982; Mergenhagen, 1984; Masada et al., 1990; Wilton et al., 1992) and its levels decrease after the appropriate treatment (Honig et al., 1989; Masada et al., 1990; Hou et al., 1995). It was reported that there is a correlation between the total amount of crevicular IL-1β concentration and both the gingival index

and the probing depth (Hou et al., 1995; Liu et al., 1996). These findings suggest that measuring the total amount of crevicular IL-1β level is very useful for monitoring the periodontal disease activity. The purpose of this study was to evaluate the effects of a carbon dioxide laser treatment on the root surfaces when used in combination with traditional gingival flap surgery using different power levels by measuring the crevicular interleukin-1β level and several other clinical parameters in patients diagnosed with adult periodontitis.

. Materials & methods A. Clinical Sampling and Design Twelve patients (5 males and 7 females) ranging from ages 29 to 52 years of age (mean: 44 years) with moderate to advanced periodontitis were selected for periodontal treatment at the Dept. of Periodontics, Dental Hospital, Yonsei University Medical Center. Informed consent was obtained from all participants. The criteria for patient selection were: 1) no history of periodontal therapy within 6 months; 2) at least 3 sites with a probing depth of 4 mm or more in each quadrant; 3) the first premolar, second premolar, first molar were retained in each of the 3 quadrants. GCF samples were collected from the site with greatest initial pocket depth of the quadrant for IL-1β analysis. Thirty-six quadrants from 12 patients were examined and three quadrants from each patient were randomly assigned to one of the following study groups: 1) flap surgery only (control); 2) flap surgery and laser treatment using an energy level of 0.8 W (group 1); 3) flap surgery and laser treatment using an energy level of 0.5 W (group 2).

Scaling and basic oral hygiene education including instructions in tooth brushing, flossing and the use of an interdental brush were given at the start of the study and the instructions were reinforced at all subsequent visits. The clinical parameters such as the probing pocket depth, the clinical attachment level, the gingival recession and the bleeding on probing were recorded at the baseline (1 month after scaling), 3 and 6 months and the GCF samples were collected at the baseline and biweekly for 6 weeks (Table 1). Table 1. Flow diagram showing the experimental procedure Baseline 2 week 4 week 6 week 3 month 6 month PD PD PD Rec Rec Rec CAL CAL CAL BOP BOP BOP GCF GCF GCF GCF PD: Pocket depth Rec: Recession CAL: Clinical attachment loss BOP: Bleeding on probing GCF: Gingival crevicular fluid

B. Clinical Parameters 1. Bleeding on probing The presence or absence of bleeding was recorded 5 seconds after removing the periodontal probe when measuring the pocket depth and attachment level. The proportion of the bleeding points out of the total number of points examined was calculated. 2. Probing pocket depth and probing attachment level The probing pocket depth and the probing attachment level were measured to the nearest of 1 mm using a Marquis color corded probe (0.5 mm in diameter). During probing, the probe was directed to the long axis of the tooth. The probe was moved twice towards the base of the pocket to find the pocket base. The distance from the pocket base to the gingival margin (probing pocket depth) and to the cementoenamel junction (probing attachment level) was recorded.

3. Gingival recession The location of the gingival margin was assessed by subtracting the figure for the pocket depth from the figure for the attachment level. C. Surgical Procedure Gingival flap surgery was employed in one side of the 3 quadrants. The two remaining quadrants were treated with a gingival flap surgery and 0.8 W/ 0.5 W CO 2 laser irradiation to the root surface after root planing. The char layer was eliminated through curettage after the laser irradiation. The wound was covered with a periodontal dressing. The patients were instructed to rinse with a 0.2% chlorhexidine digluconate solution twice daily during the first 2 weeks after surgery. They were then recalled every 2 weeks for professional tooth cleaning as described by Axelsson and Lindhe (1974). Coe Pak TM, GC America Inc, USA

D. Application of Laser The laser-treated sites were irradiated with a CO2 laser using a focused beam (2 mm from target surface), a 0.4 mm diameter focal spot, wavelength of 10.6. The laser parameters were 0.8 W (group 1) / 0.5 W (group 2) of power delivered at 50 Hz in a continuous mode. The contact optic fiber was held parallel to the root surface and moved in a back and forth motion in order to cover the entire root surface with overlapping strokes until a confluent char layer could be seen. The laser was irradiated on the alveolar bone adjacent to the root surface and the flap. When using the laser, of the smoke needs to be eliminated effectively and it is recommended that both the patient and the operator wear protective glasses to reduce the risk of eye damage, as a result of the laser and the refraction of the beam. OPELASER O3SII, Yoshida Dental MFG. CO., Japan.

E. Collection and Assay of IL-1 The GCF samples were collected by inserting paper strips into the gingival crevice until mild resistance was felt. The paper strips were kept in place for 30 seconds (Wilton et al., 1992). The fluid was sampled from a single site in each quadrant where the pocket depth measured 5-6 mm. The fluid was collected from the same site 2,4 and 6 weeks after surgery. Each paper strip was then placed in 100 of Hank's buffered salt solution (HBSS) containing 0.5% bovine serum albumin, and stored frozen at -80until needed. The amount of IL-1β levels in the GCF sample were assessed by an enzyme-linked immunosorbent assay (ELISA) with a recombinant IL-1β monoclonal antibodies. All the assay procedures were carried out according to the manufacturer's protocol and the optical densities were measured using a spectrophotometer at 450 nm. Periopaper, HARCO Electronics, Irvine, CA. Biotrak, Amersham Pharmacia Biotech UK Limited.

F. Statistical Analysis A one-way ANOVA with a Tukey's test was used to determine the statistical significance of the IL-1β levels and the clinical parameters between the different treatment groups at the baseline and at indicated time points after treatment. Repeated Measures ANOVA was used to determine the statistical significance of the IL-1β level and the clinical parameters within the groups in comparison to the baseline

. Results A. Bleeding on probing The percentage of the bleeding on probing decreased in all of the groups after the treatment. The baseline of the percentage of bleeding on probing were 80.2±19.9%, 76.2±21.5%, 86.8±21.4% respectively in group 1, group 2 and the control, respectively. It was 9.7±9.2%, 8.7±8.6%, 19.1±9.4% after 3 months and 13.6±5.2%, 15.1±4.2%, 17.5±4.8% after 6 months of treatment, respectively showing a consistent decrease every month after the treatment (p<0.05). However there were no differences among the 3 treatment groups (p>0.05). (Table 2, Figure 1) Table 2. Bleeding on probing (%) Baseline 3 months 6 months Group 1 80.219.9 9.79.2 * 13.65.2 * Group 2 76.221.5 8.78.6 * 15.14.2 * Control 86.821.4 19.19.4 * 17.54.8 * Data are represented as the proportion of bleeding site out of the total number of examined sites (%) * Statistically significant differences compared to baseline ( p<0.05).

Figure 1. Bleeding on probing (%) * Statistically significant differences compared to baseline (p<0.05). B. Probing pocket depth The probing pocket depth also decreased in all of the groups after 3 and 6 months after treatment. The baseline pocket depth was 5.0±0.6 mm, 5.2±0.6 mm, and 4.8±0.5 mm respectively in group 1, group 2 and the control respectively. It was 2.5±0.3 mm, 2.5±0.4 mm, and 2.6±0.3 mm respectively, with an approximately 2.3-2.6 mm decrease in the pocket depth after 3 months and it was 2.6±0.3 mm, 2.6±0.4 mm, and 2.8±0.3 mm respectively showing an approximately 2.1-2.6 mm decrease in the pocket depth after 6 months. All of

the groups showed a consistent decrease after the treatment. However there were no differences among the 3 groups (p>0.05). (Table 3, Figure 2) Table 3. Probing pocket depth (mm) Baseline 3 months 6 months Group 1 5.00.6 2.50.3 2.60.3 Group 2 5.20.6 2.50.4 2.60.4 Control 4.80.5 2.60.3 2.80.3 * Statistically significant differences compared to baseline ( p<0.05). Figure 2. Probing pocket depth (mm) * Statistically significant differences compared to baseline (p<0.05).

C. Clinical attachment level The clinical attachment level in all the groups decreased after 3 and 6 months after treatment consistently compared to the baseline. The results after 3 months were 3.2±1.0 mm for group 1, 3.5±1.3 mm for group 2 and 3.8±1.3 mm for the control. Group 1 and the control showed statistically significant differences in attachment gain (p<0.05), while there were no differences between group 2 and the control as well as between group 1 and group 2 (p>0.05). It was 3.3±1.0 mm, 3.7±1.4 mm and 4.0±1.3 mm in group 1, group 2 and the control after 6 months, respectively and group 1 and the control showed statistically significant differences (p<0.05), but there were no differences between group 2 and the control, group 1 and group 2 (p>0.05). (Table 4, Figure 3) Table 4. Clinical attachment level (mm) Baseline 3 months 6 months Group 1 5.30.8 3.21.0 3.31.0 Group 2 5.50.7 3.51.3 3.71.4 Control 5.00.5 3.81.3 4.01.3 * Statistically significant differences compared to baseline ( p<0.05). Statistically significant differences compared to control ( p<0.05).

Figure 3. Clinical attachment level (mm) * Statistically significant differences compared to baseline (p<0.05). ** Statistically significant differences compared to control (p<0.05). D. Concentration of IL-1β in crevicular fluid The crevicular IL-1β levels prior to the treatment for group 1, group 2 and the control were 174.6±34.2 pg/ml, 136.1±54.1 pg/ml and 188.5±92.7 pg/ml respectively. However there was a decrease after 2,4 and 6 weeks of treatment in all of the groups (p<0.05). When comparing each group according to time, there was a similar difference between group 1 and the control after 2,4 and 6 weeks of treatment, whereas there were no similar differences between group 2 and the control, and group 1 and group 2 (p>0.05). (Table 5, Figure 4)

Table 5. Changes of crevicular IL-1(pg/ml) from baseline to 6 weeks Baseline 2 weeks 4 weeks 6 weeks Group 1 174.6±34.2 22.5±14.928.5±29.020.3±24.4 Group 2 136.1±54.1 70.8±53.2 64.0±49.9 49.9±29.9 Control 188.5±92.7 110.2±72.1 98.2±64.5 82.6±43.4 * Statistically significant differences compared to baseline ( p<0.05). Statistically significant differences compared to control ( p<0.05). Figure 4. Concentration of IL-1in crevicular fluid (pg/ml) * Statistically significant differences compared to baseline (p<0.05). ** Statistically significant differences compared to control (p<0.05).

. Discussion The purpose of periodontal therapy is to make a regeneration of the connective tissue of the root surface with periodontitis in order to reproduce the periodontal tissue to allow the tooth to function normally. However, conventional scaling and root planing have some limitation in making a regeneration because it is difficult to completely eliminate plaque and calculus from the root surface. For this matter, alternatives have been examined in order to compensate for the limitations inherent in mechanical root therapy. Recently, lasers have been recommended as an alternative or adjunctive therapy in the periodontally diseased root surfaces (Iwase et al., 1989; Myers, 1991; White et al., 1991; Cobb et al., 1992). It has been suggested that a laser might be capable of sterilizing the diseased root surface and thus, and ultimately promote cell reattachment (Myers, 1992). There have been studies comparing root planing and laser in vivo (Cobb et al., 1992; Ito et al., 1993) but there are few studies investigating the effect of the laser used in gingival flap surgery. Myers et al.(1992) reported that at energy levels of 50 mj or more, the melting temperature of hydroxyapatite (700) is reached, evaporation begins,

steam forms and microexplosions occur. A series of related studies published in 1992 evaluated the effects of Nd:YAG irradiation on root specimens in vitro (Morlock et al., 1992; Spencer et al., 1992; Trylovich et al., 1992). Collectively, they reported that energy levels of 80 mj or more resulted in the formation of craters, melting and resolidification of the surface mineral, carbonization, surface porosity and peeling of the cementum. The power of the laser used in this study was 0.5 W and 0.8 W. The power recommended by the manufacturer was 0.8 W. The laser was irradiated approximately 5 times back and forth per second. Therefore, if the laser were irradiated for 0.2 second with an energy level being 0.8 W, it would be approximately 160 mj, and 100 mj for 0.5 W. According to a previous study, this is the amount that causes change in the root surface, which is less than what the product company recommended. Therefore, this study aimed to determine if there were any effects of a lower energy level as well. Therefore, laser used with a 0.5 W power was also used in the experimental group. The results evaluated by measuring the changes in the clinical parameters. There was a decrease in the percentage of bleeding on probing, the decrease in pocket depth, and a gain of clinical attachment level in all of the 3 groups

group 1, group 2 and control after 3 and 6 months of treatment and a decrease in the crevicular IL-1β levels after 2,4 and 6 weeks of treatment. Bleeding on probing is a significant clinical factor demonstrating an inflammatory lesion in the base of the connective tissue of the gingival crevice (Meitner et al., 1999). In addition, this has been acknowledged by many investigators (Hancock, 1981; Polson and Caton, 1985) that gingival bleeding acts as an indicator of the activity of the periodontal diseases. In this study, the percentage of those exhibiting bleeding on probing decreased in all of the groups after 3 and 6 months of treatment but there were no differences among the groups. The decrease in the pocket depth and the gain in the clinical attachment level play an important role in evaluating a successful treatment in patients with periodontitis. Kaldahl et al. (1988) reported that there was an average of 2.39 mm decrease in the periodontal pocket depth 3 months after flap surgery in a patient with periodontitis with a pocket depth of 5-6 mm. Becker et al. (1988) also reported that there was an average of 1.78 mm decrease, 6 months after the flap surgery in a patient with periodontitis with a pocket depth of 4-6 mm. In this study, there was a 2.26 mm and 2.05 mm decrease in the control group 3 and 6 months after the flap surgery, respectively, which was a similar

to the result reported in precious studies. There was a decrease of 2.44 mm, 2.62 mm after 3 months and 2.41 mm, 2.58 mm after 6 months in the groups using the laser with 0.8 W, 0.5 W, which showed no statistical difference from the control group. For the clinical attachment level, there was a decrease after 3 and 6 months compared to the baseline in all of the groups and in a comparison of the groups, there was a greater decrease statistically in the 0.8 W laser group than the flap surgery only group. This shows that there is no difference in the pocket depth, but there is a greater gain in the new attachment level when the 0.8 W laser is used compared to the flap surgery alone. These results were different from the previous studies. Trylovich et al. (1992) reported that when using 80 mj Nd:YAG laser on an endotoxin-treated root surface, the laser changed the biocompatibility of the cementum surface and prohibited fibroblast attachment. Gopin et al. (1997) in a histological study reported that there was a decrease in soft tissue attachment on the root surface treated with CO 2 laser in histologic study. These results seem to be due to the char layer after laser irradiation. The intense, localized heat produced when the laser comes into contact with surface debris, organic materials or a pigmented surface causes the charring. It includes remnants of ammonia, cyanate and cyanamide. It appears

that the residual char layer is a significant barrier to soft tissue reattachment. Trylovich et al. (1992) reported that in the case of a char layer in vitro, there was a suppression of fibroblast attachment. Thomas et al. (1994) reported that there was a fibroblast attachment when root planing and an air-powder abrasive slurry were used on the lased root surface in vitro. In this study, considering the results of a previous study, the char layer on the laser-treated root surface was eliminated by curettage. However, caution should be taken so as not to remove excessive cementum and dentin but to only remove the char laser gently. During the process of eliminating the char layer, it might also be effective in eliminating the areas with incomplete curettage. It is believed that one of the reasons that the laser-treated group had a greater gain in clinical attachment than the control group is due to accessibility. Irradiating the laser on a narrow intrabony defect where the curette cannot be accessed can have both a mechanical and antibacterial effect. Theoretically, a laser can eliminate plaques and calculus by ablating and vaporizing them, have access to spots where the instruments cannot reach and disinfect the pocket sulcular lining (Cobb et al., 1992; Morlock et al., 1992; Ando et al., 1996). This notion appears to be supported by our findings in that

laser (0.8 W) combined flap surgery (group 1) produces more reduction in the crevicular IL-1β and gain in clinical attachment than flap surgery only (control). The 0.5 W laser irradiation had a more decreasing effect than flap surgery only, but there did not appear to be sufficient difference. In this study, the IL-1β levels were significantly lower following CO 2 laser (0.8 W) irradiation combined with flap surgery procedure than flap surgery only. Conflicting results have been reported regarding the effect of periodontal treatment on the IL-1β levels. Some studies have demonstrated that the crevicular IL-1β levels decreased remarkably after basic periodontal therapy (Masada et al., 1990; Hou et al., 1995; Honig et al., 1995). However, Reinhardt et al. (1993) reported no significant differences in the IL-1β levels after scaling and root planing in addition to significant increases after periodontal surgery. The reasons for the difference between the different studies remains to be determined. Liu et al. (1990) also reported that in terms of the IL-1β decrease, there were no differences between the 2 groups i.e. the group that had scaling and root planing performed and the group that used laser additionally after scaling and root planing. Miyazaki et al. (2003) reported that the Nd:YAG laser alone was as effective as ultrasonic scaling alone in reducing the crevicular IL-1β

levels. However, the CO 2 laser treatment had a lesser effect on removing subgingival plaque and reducing the IL-1βlevel. This is because the CO 2 laser was used in a non-contact mode, superficially over the gingival tissue. In conclusion, this study demonstrated that a CO 2 laser (0.8 W) combined with flap surgery produces a greater reduction in the crevicular IL-1β level and clinical attachment loss than flap surgery alone.

. Conclusion The objective of the present study was to evaluate the effect of CO 2 laser treatment on the clinical parameters and crevicular IL-1β level when used in combination with gingival flap surgery. 36 quadrants from twelve patients were examined and 3 quadrants of each patient were randomly assigned to one of the 3 groups. The experimental groups received laser irradiation at 0.8 W/ 0.5 W in combination with conventional gingival flap surgery and control group received gingival flap surgery only. The results are as follows. 1. The bleeding on probing decreased in all of the groups after treatment. There were no differences among the 3 groups. 2. The pocket depth reduced in all of the groups after treatment. All of the groups showed a consistent decrease after treatment and no significant difference was found among the 3 groups. 3. The clinical attachment level decreased in all of the groups. When comparing each group, 0.8 W laser combined group showed significant decrease comparable to flap surgery only group. 4. When comparing in the crevicular IL-1β level each group according to time, 0.8 W laser combined group showed significant decrease

comparable to flap surgery only group at 2,4 and 6 weeks after treatment. These results suggest that using 0.8 W CO 2 laser additionally on root surface during the gingival flap surgery has effect on gain of clinical attachment and decrease of IL-1β level.

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Clinical photos () Figure 5-d. Figure 5-e. Figure 5-f.

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