untitled

Size: px

Start display at page:

Download "untitled"

상원 송
5 years ago
Views:

2 NA-1700

9 ,, (compulsive use) (craving). (tolerance), ( ).

10 , (,, ) ( ). (,, )

11 heroine MDMA alcohol morphine amphetamine cocaine caffeine nicotine LSD marijuana diazepam

13 Methamphetamine ( ).. methamphetamine "speed," "meth," and "chalk.". Methamphetamine hydrochloride crystal "ice," "crystal," and "glass." smoking.

16 Butorphanol Morphine Butorphanol 1. Clinically used opioid analgesic agent 2. Mixed agonist/antagonist acts on opioid receptor (agonist on kappa receptor, antagonist on mu receptor) 3. Low abuse potential 4. Chronic use: development of tolerance and dependence

18 Glutamate (% of baseline)) Increases of extracellular glutamate within the locus coeruleus during butrophanol withdrawal NLX Injection * Chronic Sal (NLX 48 nmol/5µl) Chronic Butor (NLX 48 nmol/5 µl) Time after LC naloxone injection (min)

19 Acute i.c.v. injection of butorphanol does not affect extracellular glutamate within the locus coeruleus Glutamate (% of baseline) Injection Saline Butorphanol (26 nmol/5µl) Time after acute i.c.v. injection of butorphanol (min)

20 1. Glutamate: Major excitatory neurotransmitter in CNS 2. Glutamate receptor: NMDA, AMPA, Kainate 3. NMDA receptor : Involved in the phenomena of opioid dependence and withdrawal Glu Glu Glu Glu GluCa 2+ NMDA R Ca 2+ Ca bnos 2+ Arginine NO - + Altered gene expression Nucleus

21 Experimental Protocol SD rats g 1 week 3 days Surgery for i.c.v. Implant Osmotic pump Butorphanol (26 nmol/ µl/hr) Brain section (20 um) for Autoradiography In situ hybridization

23 Chronic microinfusion into icv by using osmotic minipump Butorphanol was infused (26 nmol/1 l/hr) for 3 days

Autoradiography of [ 3 H]MK-801 50 mm Tris-HCl 10 nm [

24 Autoradiography of [ 3 H]MK mm Tris-HCl 10 nm [ 3 H]MK-801 Incubate : 120 min at 25 Film expose : 4 weeks

25 Conventional film and densitometry film slide latent image development & fixation autoradiograph computer with image acquisition software A/D converter stable light source (laser scanning)

26 Cortex CPu Thalamus Hippocampus DG Cerebellum

27 Changes of [ 3 H]MK-801 binding by butorphanol Control Tolerance Withdrawal

28 ** ** ** **

30 TREATMENT

Prescription of NA1700 Greenlife World Inc. ( ) NA1700 (COEX) 1.Orange Peel...163mg 2.Penny Root 160mg 3.Angelica Herb... 120mg 4.Sandalwood.. 120mg 5.Poria...80mg 6.Common Motherwort.80mg 7.Licorice.

31 Prescription of NA1700 Greenlife World Inc. ( ) NA1700 (COEX) 1.Orange Peel...163mg 2.Penny Root 160mg 3.Angelica Herb mg 4.Sandalwood.. 120mg 5.Poria...80mg 6.Common Motherwort.80mg 7.Licorice...80mg 8.Valeria Root... 62mg 9.Geranium mg 10.Piperis Nigris Fructus...35mg 11.Peppermint....20mg 12.Cinnamon.. 20mg 13.Cystein..qs 14.Glutathione..qs Total 1000mg/capsule

32 NA1700 suppress the nalbuphine-induced withdrawal signs

33 NA1700 inhibits the nalbuphine-induced perk expression perk (optical density) Cortex * * 44KDa 42KDa perk (optical density) Hippocam 44KDa 42KDa 0 0 Saline Nalbu NA1700 Nalbu+NA Saline Nalbu NA1700 Nalbu+NA perk (optical density) Striatum * 44KDa 42KDa 44pERK 42pERK 0 Saline Nalbu NA1700 Nalbu+NA

34 NA1700 inhibits the methamphetamine-induced CPP 200 ** Time in Sec Sal Sal NA 250 NA 500 Meth 2mg/kg

35 NA1700 decreases the apomorphine-induced locomotion in methamphetamine treated rats Locomotor Activity (Counts/min) Total * + + Sal Meth NA250 NA500 Methamph Locomotor Activity (Counts/min) Time Minutes sal Meth NA Meth NA Meth

36 Clinical approach with NA1700 Tx) Treat the 110 abusers with NA1700 for 3-7 days 1) Martin Luther King Jr. Medical Center (U.S.A.) (5 ) 2) A.P.Y Lab (Israel) (10 ) Cocaine, heroine addiction 3) Army Hospital (Miyanma) (16 ) 4) Rumah Sakit Graha Medika (Indonesia) (46 ) Opium, methamphetamine, heroine addiction 5) Ansan City Hospital (China) (30 ) 6) Specialist Internal Medicine (Iran) (3 ) Opium, heroine, methadone, Marijuana addiction Ameliorate the withdrawal syndrome Abolish the rewarding Greenlife World Inc. ( )

37 Clinical approach with NA1700 Rumah Sakit Graha Medika, Indonesia Greenlife World Inc. ( )

38 Clinical approach with NA1700 Greenlife World Inc. ( )

39 NA1700 Greenlife World Inc. ( )

40 NA1700 NA1700 (COEX) NA1700 NA1700, :00AM 2:00PM 6:00PM 10:00PM 2:00AM 6:00AM 10:00AM Greenlife World Inc. ( )

41 NA1700 Greenlife World Inc. ( )

42 NA1700 NA Greenlife World Inc. ( )

43 CONCLUSION

44 These results indicate that Natural product (NA1700) could be developed as good anti-narcotic medicine without a severe side effect.

45 NA NA NA pool.

슬라이드 1

슬라이드 1 Opioids: Blessing and Curse Ki-Wan Oh, PhD. College of Pharmacy, Chungbuk National University Among the remedies, which it has pleased almighty God to give to man to relieve his sufferings, none is so