CERTIFICATE OF STRAIN This is to certify that the starting breeders of the following strain have been supplied by Taconic Farms, Inc. to Samtako Bio K

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1 (주)샘타코바이오코리아 우) 경기도 오산시 서랑동 77-1 / Tel : ~ 8 / Fax : 문서번호 : STK 날 짜 : 수 신 : 실험동물연구원 제위 제 목 : DB 마우스 국내 생산 공급 관련. 1. 귀사(원)의 무궁한 발전을 기원합니다. 2. 당사는 엄격한 barrier system하에서 고품질의 실험동물을 생산 관리하고 있으며, 무엇보다 국내에 고품질의 실험동물 공급을 위하여 품질 관리와 생산성 향상에 매진하고 있음을 자부합니다. 3. 국내 실험동물 연구의 지속적인 요구 사항에 맞추어 당사는 품종의 다양화의 일환으로 213년도부터 db/db 마우스를 당사의 엄격한 system 하에서 생산하여 공급하여 드림을 알려드립니다. 1) 모체 공급사 : Taconic 2) 공급 시기 : 즉시 3) 공급 단가 : strain 주령 (weeks) 단가 (원) 비고사항 4 8, db/db 9, 6 1, 7 11, 1 8주령 이상 동물에 대해서는 별도 문의 2 마리 이하 주문시 박스값 별도 부과 4) Strain Certificate 및 동물 관련 정보는 첨부된 자료를 참고하여 주시길 바랍니다. ) 자체 혈당 및 체중 측정 데이터는 첨부된 자료를 참고하여 주시길 바랍니다. 6) 미생물 모니터링 및 유전 모니터링 report에 대해서는 당사에 별도로 문의 주시길 바랍니다. 4. 앞으로도 지속적으로 당사에 대한 관심과 지원을 부탁드립니다. 감사합니다. - 첨부 : 1 Strain Certificate (1장) 2 DB 마우스 Description (1장) 3 자체 혈당 및 체중 측정 데이터 (4장) - 이 상 - (주)샘타코바이오코리아 (직인생략)

국내 실험동물 연구의 지속적인 요구 사항에 맞추어 당사는 품종의 다양화의 일환으로 213년도부터 db/db 마우스를 당사의 엄격한 system 하에서 생산하여 공급하여 드림을 알려드립니다.

2 CERTIFICATE OF STRAIN This is to certify that the starting breeders of the following strain have been supplied by Taconic Farms, Inc. to Samtako Bio Korea on December 212, and have been expanded and maintained under Samtako SOP. Strain Name: DB Formal Nomenclature: BKS.Cg-m+/+Lepr db /BomTac Origin: The Diabetic spontaneous mutant model was developed by Hummel et al. in 199 by discovering a Lepr db mutation on a C7BLKS background. M&B A/S (now Taconic Europe) received stock from CERJ France in The mice were derived by embryo transfer in 2 at Taconic US and are maintained by incrossing brother x sister mice. Color: Black (Homozygous and Heterozygous) and Misty (Grey) (Wild) Ana V. Perez, Ph.D. Genetics Department Head Taconic Farms, Inc. One Hudson City Centre, Hudson, NY 1234, USA Corporate Office - One Hudson City Centre Hudson, NY 1234 Tel: 888.TACONIC Fax: custserv@taconic.com European Office Tornbjergvej 4, Ejby 4623 Lille Skensved, Denmark Tel: Fax: taconiceurope@taconic.com

Cg-m+/+Lepr db /BomTac Origin: The Diabetic spontaneous mutant model was developed by Hummel et al. in 199 by discovering a Lepr db mutation on a C7BLKS background.

3 Diabetic SPONTANEOUS MUTANT DIABETES/OBESITY MICE BKS Background Model Description A suitable model for the study of diabetic neuropathology and nephropathy Also used in the study of the pathogenesis and prevention of Type II Non-Insulin Dependent Diabetes Mellitus (NIDDM) Experiences a hyperinsulimemic phase (Phase 1) followed by a hypoinsulimemic phase (Phase 2) at 2-3 months of age Due to possible crossover of the misty (m) and Leprdb genes, a low percentage of DB-M could be heterozygous for the misty (m) gene Only homozygotes (black) are diabetic and obese, heterozygotes (black) and wild types (misty) are non-diabetic and lean *Pricing is for ungenotyped lean or obese animals. Customers wanting genotyped animals must allow for an extra 2 week leadtime. Origin : The Diabetic Spontaneous mutant model was developed by Hummel et al in 199 by discovering a Lepr db mutation on a C7BLKS background. M&B A/S (now Taconic Europe) received stock from CERJ France in The mice were derived by embryo transfer in 2 at Taconic US and are maintained by incrossing brother x sister mice. Color : Black Genetics : Strain Profile: Akp1 a, Akp2 a, Anpep a, Car2 a, Es1 a, Es2 b, Es3 a, Es b, Es9 a, G6pd1 a, Gpi1 b, Got2 b, Hbb s, Idh1 b, Ldr1 a, Ldr2 a, Mod1 b, Mup1 b, Pep3 b, Pgm1 a, Trf b Immunology: H2 d Mutation: Lepr db, m

possible crossover of the misty (m) and Leprdb genes, a low percentage of DB-M could be heterozygous for the misty (m) gene Only homozygotes (black) are diabetic and obese, heterozygotes (black) and

4 Blood sugar diagram -Samtako IBRS: 32 Sex: Female Measuring Instrument Roche ACCU-CHEK Performa Nano Strain: Diabetic Quantity: Fasted time 16 Hours FEED HI Average Lower dev Upper dev FASTED Average Lower dev Upper dev Blood sugar diagram Feed Fasted

2 38.1 9.7 4.2 3.7 FASTED 1 118 337 384 398 39 29 24 2 1 332 348 344 138 4 187 3 199 318 31 294 219 19 1 4 162 217 289 97 16 36 344 2 276 311 4 286 234 476 Average 16.8 296. 336.6 37.6 31.8 33.6 283.

5 Blood sugar diagram-samtako IBRS: 32 Sex: Male Measuring Instrument Roche ACCU-CHEK Performa Nano Strain: Diabetic Quantity: Fasted time 16 Hours FEED HI HI Average Lower dev Upper dev FASTED Average Lower dev Upper dev Blood sugar diagram Feed Fasted

8 7. 48.3 FASTED 1 112 26 41 284 389 472 331 2 12 14 462 212 461 249 338 3 28 41 326 148 473 262 234 4 17 174 442 22 347 191 212 13 241 382 392 332 294 327 Average 12.8 244.2 412.6 311.6 4.4 293.

6 Weight in grams Weight diagram-samtako IBRS: 32 Strain: DB Quantity: Sex: Female Q per cage: Reg. Nr. Age [weeks] Average Lower dev Upper dev Mean Weight ± Standard Deviation

4 38.8 39.2 4.1 47.4 49.7 49.2 Average 33.9 37.4 39.2 4.2 4.1 46.6 47.8 Lower dev. 31. 34.6 36.8 38.8 42.8 43.4 46.

7 Weight in grams Weight diagram-samtako IBRS: 32 Strain: DB Quantity: Sex: Male Q per cage: Reg. Nr. Age [weeks] Average Lower dev Upper dev Mean Weight ± Standard Deviation

4 44.6 4.2 43.6 39.3 43.2 42.7 43.4 46.8 48.1 44.1 Average 37.3 41. 41. 4. 44.7 47.2 43.4 Lower dev. 33.2 37. 38.6 43.

012임수진

012임수진 Received : 2012. 11. 27 Reviewed : 2012. 12. 10 Accepted : 2012. 12. 12 A Clinical Study on Effect of Electro-acupuncture Treatment for Low Back Pain and Radicular Pain in Patients Diagnosed with Lumbar