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Lee, Geon Kook None This project was designed to establish the Tumor Bank of National Cancer Center in 2000. From the first tumor sample in 2000, the total of tumor and tumor-related samples has been increased up to 110,547 vials of snap-frozen tumor tissue and 26,463 methacarn-fixed paraffin-embedded tissue blocks from a total of 14,208 cases of the common malignant tumors. Blood-derived samples has been stored from a total of 26,270 cancer patients from 2000. During this project period, from 2010 to 2012, 21,283 vials of snap-frozen tumor and matched normal tissues and 1,621 methacarn-fixed paraffin-embedded tissue blocks have been collected and stored from 3,433 cases of the common human malignant tumors. The peripheral blood has also been sampled from 3,244 patients with malignant tumors, and each component of serum, plasma and white blood cells (buffy coat) is separated and stored in liquid nitrogen tank. Quality of the stored tissue have been tested and assured at the level of DNA, RNA and protein by PCR, RT-PCR and immunoblot. During the last 3-year-period, primer sets for tumor suppressor genes and antibodies against the products of the same tumor suppressor genes were added to quality control and quality assurance protocol. We have been having the regular meeting of NCC Bio Bank Management Committee and started the distribution of the stored samples from 2008. For the 28 research projects, 6,057 blood-derived the products (serum 1,748vials, plasma 366 vials and buffy coat 3,943 vials) and 2,027 tissue samples (normal 764vials and tumor 1,263vials). In addition, we have been construct patient clinically information program which are related to the stored tumor samples. We hereby can control total sample information including sample, pathological and clinically information. l l

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Stati on Solution Time Temp. Pressure & Vacume Agitation 1 100% alcohol 1:00 4 Manual Manual 2 100% alcohol 1:00 4 Manual Manual 3 100% alcohol 1:00 4 Manual Manual 4 100% alcohol Variable Room temp. On On 5 Xylene 1:00 Room temp. On On 6 Xylene 1:00 Room temp On On 7 Xylene 1:00 Room temp On On 8 Paraffin 0:30 60 On On 9 Paraffin 0:30 60 On On 10 Paraffin 1:00 60 On On 11 Paraffin 1:00 60 On On μ

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l l Lee, Geon Kook None This project was designed to establish the Tumor Bank of National Cancer Center in 2000. From the first tumor sample in 2000, the total of tumor and tumor-related samples has been increased up to 110,547 vials of snap-frozen tumor tissue and 26,463 methacarn-fixed paraffin-embedded tissue blocks from a total of 14,208 cases of the common malignant tumors. Blood-derived samples has been stored from a total of 26,270 cancer patients from 2000. During this project period, from 2010 to 2012, 21,283 vials of snap-frozen tumor and matched normal tissues and 1,621 methacarn-fixed paraffin-embedded tissue blocks have been collected and stored from 3,433 cases of the common human malignant tumors. The peripheral blood has also been sampled from 3,244 patients with malignant tumors, and each component of serum, plasma and white blood cells (buffy coat) is separated and stored in liquid nitrogen tank. Quality of the stored tissue have been tested and assured at the level of DNA, RNA and protein by PCR, RT-PCR and immunoblot. During the last 3-year-period, primer sets for tumor suppressor genes and antibodies against the products of the same tumor suppressor genes were added to quality control and quality assurance protocol. We have been having the regular meeting of NCC Bio Bank Management Committee and started the distribution of the stored samples from 2008. For the 28 research projects, 6,057 blood-derived the products (serum 1,748vials, plasma 366 vials and buffy coat 3,943 vials) and 2,027 tissue samples (normal 764vials and tumor 1,263vials).

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Stati on Solution Time Temp. Pressure & Vacume Agitation 1 100% alcohol 1:00 4 Manual Manual 2 100% alcohol 1:00 4 Manual Manual 3 100% alcohol 1:00 4 Manual Manual 4 100% alcohol Variable Room temp. On On 5 Xylene 1:00 Room temp. On On 6 Xylene 1:00 Room temp On On 7 Xylene 1:00 Room temp On On 8 Paraffin 0:30 60 On On 9 Paraffin 0:30 60 On On 10 Paraffin 1:00 60 On On 11 Paraffin 1:00 60 On On μ l l

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2004 년 1 월 29 일제정, 2005 년 1 월 1 일부터시행된 < 생명윤리및안전에관한법률 > 에근거