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Heraeus Gassed Incubator Series 6000 BB 6060, 6220 operation manual Heraeus Homepage wwwbarnsteadthermolynecom Distributor (Tel 577-6212, ext5) Homepage wwwdaeilsciencecokr

1 Control panel Display and Keys Display: display, setpoint setpoint key Temp-related error codes [i] key display Special functions [ Cf Function Selection (p21) ] key % CO 2 Display: display, setpoint setpoint key CO 2 -related error codes [i] key display Special functions [ ] key Cf Function Selection % O 2 Display: display, setpoint setpoint key O 2 -related error codes [i] keydisplay Setpoint Keys: Temperature, CO 2 (O 2 )* [UP] [DOWN] Keys: [i] key: Error code display [ ]key : Special function function selection *=Optional 2

LED AUTO-ZERO: CO 2 measuring system LED AUTO-CAL*: O 2 measuring system LED KCL*: LED Gas: Gas LED Alarm: Alarm LED Control: Special function Temperature limit controller Cut-off point dial on/off Master switch Fuses *=Optional 3

2 Introduction gassed incubator manual No50031065 Incubator Heraeus Customer Service technical expert, spare parts nameplate data : * DIN 58 945 * DIN 12 880 Part 1 * DIN VDE 0700 Part 1;IEC 335-1 VDE Inspection Office GS-label accwdin VDE 0871, class B, VDE Inspection Office 3 Application Gassed Incubator incubator Heraeus Service Agent 4

4 DESCRIPTION OF THE UNIT Heating System Air-jacket heating system water reservoir condensation system condensation condensation, switch ON, heater 5

Gas supply Gas (CO 2, O 2, N 2 ) Gas inlet pressure 1 bar Gas, gas filters(03 Filter99998% Gas analyzer fan gas incubator Pressure compensation pressure compensation vent gases vent check valve Valve check valve gas valve 6

Option Microperlator (O 2 Version) Water reservoir microperlator( hose) O 2 N 2 transfer gas 7

DESCRIPTION OF THE UNIT Microprocessor-based control system : system a) Physiol Parameter Control Setting ranges: * Temperature: 70 500 * CO 2 content : 00 200 % * O 2 content : 30 800 % (Optional) b) Automatic Calibration of the Meas Systems * AUTO-START routine CO 2 AUTO-ZERO system O 2 AUTO-CAL system (Optional) c) Monitoring Functions * Door Control * Error Messages * Error Storage * External Alarm System (Optional) * Error Code Table d) Special Functions * Buzzer : ON/OFF * Gas Supplies : ON/OFF * Setpoint Key : Lock-in * Operat Modes : with or w/o water 8

a) Physiol Parameter Control Temperature : microprocessor-based control Pt 100 sensor sensor heating system, alarm CO 2 : ph setting CO 2 CO 2 ph sodium bicarbonate buffer graph Appendix CO 2 CO 2 CO 2 O 2 : Optional in vitro (30% 210%) (210% 800%) CLARK cathode anode 03mol KCl O 2 control Socket (cf Maintenance Instructions, p25) Gas Mixtures : Microprocessor gas CO 2 O 2 setting 95% (O 2 concentration max 80 %) 9

b) Automatic Calibration of the Mesa Systems AUTO-START Routine start-up setpoint system Incubator AUTO-START routine microprocessor, system, process 15 AUTO-START gas (p 19) CO 2 AUTO-ZERO System detectors 35 process 7 LED AUTO-ZERO AUTO-ZERO gas O 2 AUTO-CAL System (Optional) AUTO-ZERO routine sensor pump microprocessor 21% LED AUTO-CAL AUTO-CAL routine 10

c) Monitoring Functions Error Messages: Microprocessor error code numbers code number error Affected control circuit display displays( ) LED Alarm system error buzzer, [i]keyerror error code affected control circuit displays, displays [i]key displays [---] Reminder, [i] ley, error code displays Error message : * Setpoint changes * Turning the unit off/on Error Storage: Service work, 9 errors error memory memory, Service Technician External Alarm system (Optional) monitoring system (telephone system building alarm system ) 11

Error Code Table : (SP=Setpoint) Error Code Possible Cause Remedy 99 100 (SP) 101 (SP) Templimit controler Temp limit controller ; (p20) switch 104 broken sensor Temperature sensor Service Technician 200 CO 2 (SP) 201 CO 2 (SP) CO 2 CO 2 cylinder CO 2 inlet CO 2 inlet 202 Gas analyzer calibr Error Hose Hose Gas supply cf Installation CO 2 cylinder inlet ; 1bar inlet ; 1bar Gas analyzer fan Fan, identification rings Service Technician 203 Gas analyzer Gas analyzer Gas analyzer 300 N 2 (SP) 301 N 2 (SP) 302 O 2 (SP) 303 O 2 (SP) N 2 N 2 cylinder N 2 inlet N 2 inlet O 2 O 2 cylinder O 2 inlet O 2 inlet 304 Electrolyte error Electrolyte KCl level foil membrane 305 O 2 electrode Electrolyte level foil membrane plug Gas supply cf Installation N 2 cylinder Inlet ; 1 bar inlet ; 1 bar Gas supply cf Installation O 2 cylinder Inlet ; 1 bar Inlet ; 1 bar Electrolyte cfappendix Electrode cf Appendix Electrode Install cf Appendix Electrolyte cfappendix Electrode Assy Instruct, cf Appendix 12

d) Special Functions p21 [ ] Function Key Buzzer ON/OFF Function 1: buzzer, error message Buzzer OFF: Silent alarm Factory Setting : Buzzer ON Gas ON/OFF Function 2: Gas OFF, gas supplies ; LED Gas Factory Setting : Gas ON; LED Gas Setpoint Lock-in Function 4:, CO2 O2 setpoint Factory Setting : Setpoints Operating Modes Functions 7: reservoir, AUTO- START routine mode Factory Setting: reservoir 13

5 OVERTEMPERATURE PROTECTION Temperature Limit Controller (responds to temperature) incubator, sample electronic temperature limit controller ( accwdin 12 880 Thermal Safety Class 31) Temperature control circuit, (,) temperature limit controller control setting (coin screwdriver); cf Startup(p19) Protection of the Unit 55 (limit stop) control knob, temperature limit controller incubator Load Protection microprocessor 1~2, Temperature limit controller sample Temperature limit controller Temperature limit controller control, Error Code Table Operational Test temperature limit controller display 2 dial External Alarm System 14

6 Placement and Installation Location : accessories Incubator, 30 setting ( feet) Incubator CO 2, O 2 N 2, bench install gases Appendix Clearances Incubator / check valve valve hose (condensation ) 15

Installation under LabBenches Installation * gas supplies * supply * supply cable gas supplies lines bench Stacking of gassed Incubator 16

Installation, Connection to the Gas Supply hose gas 1 bar pressure relief valve ( ) 1 bar setting CO 2 Gas cylinder gas nozzle O 2 (Optional) 21% cylinder N 2 (Optional) 21% cylinder Reminder: Gas 995% CO 2 cylinder Riser gas nozzle hose Connection to the Mains mains, nameplate plug cable 16 A delay-action type fuse fusing Gas Analyzer hose board plug-in (illustration ; color-coded nozzles!) 17

Mikro-porous Hose Connection(O 2 Version) (option) microperlator Water reservoir hose gas inlet nozzle (illustration p7) Oxygen eletrode support ( Appendix p30) hose board, jack 18

7 START-UP Operation with Distilled Water controller 8 * ( Model BB6220 glass-screen ) * water reservoir : BB 6060-4l BB 6220-6l Operation w/o Distilled Water * Special Function Table Function Code 7, OperatMode w/o water [UO] culture dishes * manual gas supply shut-off valves * master switch - - Digital displays [888] 40 - Auto-test routine - auto-test routine displays 19

Setpoint Adjustment Setpoint Keys [ ] [CO 2 ] [UP] [DOWN] keys [O 2 ] * setpoint setpoint keys [ ], [CO2], [O2] : - setpoint display - Display - [UP] [DOWN] key setpoint setpoint key setpoint display Factory-Settings Temperature : 370 CO 2 : 00 % O 2 : 210 % Adjustment of the TempLimit Controller 1~2 Cf Overtemperature protection p14 AUTO-START! gas * [UP] [DOWN] keys 5 - AUTO-START routine CO 2 display [00] - LED gas - O 2 display [210] (option) * Reminder: glass screen setpoint, AUTO-START routine( 15 ), system CO 2 /O 2 AUTO- ZERO/AUTO-CAL routine - LED AUTO-ZERO, AUTO-CAL - display - LED gas - CO 2 O 2 setpoint, gas 20

- [21/0%] O 2, O 2 setting - sample Special Functions [ ] Key [UP]/[DOWN] keys [ ] Key [UP]/[DOWN] key [ ]display [ 1] function code [, CO 2 display ] key, Function Code [ ] Temperature Display [ ] Display [CO 2 ] 1 Buzzer [1] [A1] [A0] 2 Gas [2] [G1] [G0] 4 Setpoint Release [ 4] [S0] [s1] 7 Operat Mode [7] [U1] [U0] Function Buzzer ON Buzzer OFF Gases ON Gases OFF Not released Released w water w/o water Factory Setting Buzzer ON Gases ON Released w water p13 21

Notes: Temperature: setpoint 1, gas detector start-up chapter CO 2 Comparison CO 2 CO 2 system Reference example: CO 2 display: 70 % CO 2 CO 2 measured : 62 % CO 2 * function code 3 [ ] [UP] keys * [ ] key * [ ] key - temperature display [ 0], - CO 2 display [ 1] * temperature display [UP] key : [62] * [ ] key * [i]key (microprocessor computation ) CO 2 display computation display setpoint, gas 15 22

Admittance of O 2 : O 2 electrode display * jack * 15, system AUTO-CAL routine, setpoint General Remarks: * reservoir, * O2, (Appendix ) * *, [i] key, display buzzer, [i] key display code number (cf Error Code Table p12) [I]key display [---] 23

Malfunctions which are not displayed: Malfunction * * gas Remedy, Checkout - setpoint - LED gas gas -setpoint * Buzzer -buzzer * Setpoints cf Special Functions p21 - setpoints cf Special Functions p21 * glass pane condensation - switch p5 Shutdown of the Unit * master switch * manual gas valve 24

8 Disinfection, Cleaning, Checks Weekly checks to be performed by the User: * reservoir * gas 1 bar * O 2 : vent hole 15mm (cf Appendix) Disinfection and cleaning of the chamber by the user: * * screwdriver glass screen (Model 6220) * * O2, gas analyzer, O 2 electrode Removal (Optional) * plug * O2 socket * hose nozzle * * O 2, Removal of the shelf Supports Unit with full-width shelves * * * carrier rack * carrier rack 25

unit with divided shelves: *, carrier rack * carrier rack * carrier rack (spring) Removal of the Gas Analyzer * socket plug * hose * thumb screw * gas analyzer, Reminder Gas analyzer Heraeus Removal of the Glass Screen, Model BB 6220 Only, * glass screen screwdriver * door hinge guard ring * glass screen hinge Recommended Disinfectant Barrycidal 36, available form: Interchem AG Graben 5 CH-6300 Zug, Switzerland Phone 042 22 16 24 Telefax 042 22 19 75 Telex 042 868 988 icag ch Alcohol* Service 26

Maintenance Heraeus-authorized personnel Heraeus :, Spare parts lists Service department Heraeus agent 27

9 ADDITIONAL EQUIPMENT(OPTIONS) UV Radiator (Optional) UV radiator radiator radiator, radiator UV radiator, : * mains * radiator Start-Up * master switch * * * manual timer UV Timer switching interval * UV radiator key switch key * UV radiator - radiator - UV, UV radiator UV radiator service Technical Data Resonance line 254 mm 28

high/low Humidity System Radiator efficiency 15 W Model BB 6220 Position system 92% position 95% culture dishes,, system position dishes condensation position dishes, condensation dishes Position lamp 29

APPENDIX Instructions for the Installation of the O 2 Electrode O 2 Electrode Ass y No 26136-184 Comprises: 1 O2 electrode compl 26 136 180 1 spare adapter sleeve 26 136 329 1 spare wire clip 03 579 098 1 mounting ring 03 669 999 10 sets of foil membrane 26 136 339 1 bottle KCL solution 03 Mol 26 136 157, O 2 1 premounted electrode wire clip(1) 2 foil membrane(2) plastic bag adapter sleeve(3) R 3 mounting ring(3) foil electrode sheath(5) adapter sleeve foil 4 wire clip 5 KCL solution 85ml 6 filtering clean rag platinum cathode(7) Cathode 7 electrode(6) Vent hole 1~3 mm KCL solution Electrode sheath Platinum cathode foil membrane 8 wire clip 9 electrode support bracket 30

Operation Instruction Reservoir BB 6060:ca 4l BB 6220:ca 6l Page 19 Page 19 Setpoint fex setpoint key setpoint UP/DOWN key Page 20 temperature limit controller Page 14 Auto-Start * CO2 display Page 20 fex 15 Illum LED Auto-Start Displays Error Report: display Control circuit Error Code Table Page 12 fex key alarm, key 31

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