203044-3.hwp

(a) Xbal BamHll pbi101 Stsy(896bp) GUS(1800bp) Nos3 GTCGACTCTAGACTGAAA-----------ATGGCTGGATCCCCGGGTGGTCAGTCCCTTATGTTACGT XbaI BamHI GUS start codon

250 200 intensity 150 100 50 0 1 2 3 4 5 6 7 8 9 10 11 12 stressed sample

intensity 180 160 140 120 100 80 60 40 20 0 1 2 3 4 5 6 7 8 9 stressed sample

intensity 80 70 60 50 40 30 20 10 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 stressed sample

intensity 90 80 70 60 50 40 30 20 10 0 1 2 3 4 5 6 7 8 9 10 11 12 stressed sample

UV stress처리 후 relative concentration 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0 UV 0min 처리 후 0h UV 0min 처리 후 24h UV 0min 처리 후 48h UV 5min 처리 후 0h UV 5min 처리 후 24h UV 5min 처리 후 48h UV 10min 처리 후 0h UV 10min 처리 후 24h UV 10min 처리 후 48h UV 30min 처리 후 0h UV 30min 처리 후 24h UV 30min 처리 후 48h MJ와 UV stress처리 후 relative concentration 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0 Cont 1h MJ 1h UV15min+MJ 1h Cont 6h MJ 6h UV15min+MJ 6h Cont 24h MJ 24h UV15min+MJ 24h

SA stress처리 후 relative concentration 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0 Cont 1h SA 100μM1h SA 200μM1h SA 500μM1h Cont 6h SA 100μM6h SA 200μM 6h SA 500μM 6h Cont 24h SA 100μM 24h SA 200μM 24h SA 500μM 24h

1.2 BA stress 처리 후 relative concentration 1 0.8 0.6 0.4 0.2 0 Cont 1h BA 50μM1h BA100μM1h BA200μM1h Cont 6h BA 50μM6h BA100μM6h BA200μM6h Cont 12h BA 50μM12h BA100μM12h BA200μM12h Cont 24h BA 50μM24h BA100μM24h BA200μM24h

9 8 7 6 5 4 3 2 1 0 UV 0min 처리 후 0h UV 0min 처리 후 24h UV 0min 처리 후 48h UV 5min 처리 후 0h UV stress처리에 따른 건중량μg/g비교 UV 5min 처리 후 24h UV 5min 처리 후 48h UV 10min 처리 후 0h UV 10min 처리 후 24h UV 10min 처리 후 48h UV 30min 처리 후 0h UV 30min 처리 후 24h UV 30min 처리 후 48h MJ와UV stress처리에 따른 건중량μg/g 비교 4.5 4 3.5 3 2.5 2 1.5 1 0.5 0 Cont 1h MJ 1h UV15min+MJ 1h Cont 6h MJ 6h UV15min+MJ 6h Cont 24h MJ 24h UV15min+MJ 24h

SA stress처리에 따른 건중량μg/g비교 5 4.5 4 3.5 3 2.5 2 1.5 1 0.5 0 Cont 1h SA 100μM1h SA 200μM1h SA 500μM1h Cont 6h SA 100μM6h SA 200μM 6h SA 500μM 6h Cont 24h SA 100μM 24h SA 200μM 24h SA 500μM 24h 7 6 5 4 3 2 1 0 Cont 1h BA 50μM1h BA100μM1h BA200μM1h BA stress처리에 따른 건중량μg/g비교 Cont 6h BA 50μM6h BA100μM6h BA200μM6h Cont 12h BA 50μM12h BA100μM12h BA200μM12h Cont 24h BA 50μM24h BA100μM24h BA200μM24h

50.0 40.0 30.0 20.0 10.0 0.0 UV 또는 SA 혼용 처리 후 저장 기간과 온도에 따른 relative concentration control UV 30min 4 저장 UV 30min RT 저장 UV 30min + SA 100uM RT 저장 1일 2일 3일 4일 5일 UV 또는 SA 혼용 처리 후 저장 기간과 온도에 따른 resveratrol contents(mg/g) resveratrol contents( 0.150 0.100 0.050 0.000 control UV 30min 4 저장 UV 30min RT 저장 UV 30min + SA 100uM RT 저장 1일 2일 3일 4일 5일

0.10 0.08 resverat 0.06 0.04 0.02 0.00 control YE 0.1% YE 0.5% YE 1% YE 2%

resveratrol contents 0.20 0.15 0.10 0.05 0.00 control UV 30min UV 30min + YE 0.1% 1회 분무 UV 30min + YE 0.1% 2회 분무 UV 30min + YE 0.1% 3회 분무 UV 30min + YE 0.1% 4회 분무

resveratrol contents( 1.200 1.000 0.800 0.600 0.400 0.200 0.000 2일 5일 control UV 5' UV 15' UV 30' UV 45' UV 60' resveratrol contents( 1.600 1.400 1.200 1.000 0.800 0.600 0.400 0.200 0.000 control UV-4 UV-18 UV-23 UV-28 UV-37

resveratrol contents( 0.800 0.700 0.600 0.500 0.400 0.300 0.200 0.100 0.000 control UV 30' 1일 UV 30' 2일 UV 30' 3일 UV 30' 4일 UV 30' 5일

resveratrol contents 250.00 200.00 150.00 100.00 50.00 0.00 0일 1일 4일 5일 8일 9일 13일 14일 18일 19일 23일 control 4 RT and 4 alternative

Sphericity 0.940 0.930 0.920 0.910 0.900 0.890 0.880 0.870 0.860 0.850 Control Plain water Ozone water Not stored 5-days Sonication Moisture content (%) 80.0 79.0 78.0 77.0 76.0 75.0 74.0 Control Plain water Ozone water Sonication Not stored 5-days

L value 33.00 32.50 32.00 31.50 31.00 30.50 30.00 29.50 29.00 28.50 Control Plain water Ozone water Not stored 5-days Sonication a value 5.00 4.50 4.00 3.50 3.00 2.50 2.00 1.50 1.00 0.50 0.00 Control Plain water Ozone water Sonication Not stored 5-days b value 0.00-0.20-0.40-0.60-0.80-1.00-1.20-1.40-1.60-1.80 Control Plain water Ozone water Sonication?E 33.00 32.50 32.00 31.50 31.00 30.50 30.00 29.50 29.00 28.50 Control Plain water Ozone water Sonication Not stored 5-days Not stored 5-days 3.5 80.0 Rupture deformation (mm) 3.0 2.5 2.0 1.5 1.0 0.5 Rupture force (g) 70.0 60.0 50.0 40.0 30.0 20.0 10.0 0.0 Control Plain water Ozone water Sonication 0.0 Control Plain water Ozone water Sonication Not stored 5-days Not stored 5-days Soluble solids (%brix) 20.5 20.0 19.5 19.0 18.5 18.0 Resveratrol content (μg/g) 6.00 5.00 4.00 3.00 2.00 1.00 17.5 Control Plain water Ozone water Sonication 0.00 Control Plain water Ozone water Sonication Not stored 5-days Not stored 5-days

Sphericity 0.900 0.890 0.880 0.870 0.860 0.850 0.840 0.830 0.820 Control Plain water Ozone water Not stored 5-days Sonication Moisture content (%) 82.0 80.0 78.0 76.0 74.0 72.0 70.0 68.0 66.0 64.0 62.0 Control Plain water Ozone water Sonication Not stored 5-days 33.00 6.00 32.00 5.00 L value 31.00 30.00 29.00 a value 4.00 3.00 28.00 2.00 27.00 Control Plain water Ozone water Not stored 5-days Sonication 1.00 0.00 Control Plain water Ozone water Sonication Not stored 5-days b value 1.80 1.60 1.40 1.20 1.00 0.80 0.60 0.40 0.20 0.00-0.20 Control Plain water Ozone water Sonication?E 33.00 32.00 31.00 30.00 29.00 28.00 27.00 Control Plain water Ozone water Sonication Not stored 5-days Not stored 5-days Rupture deformation (mm) 2.5 2.0 1.5 1.0 0.5 Rupture force (g) 120.0 100.0 80.0 60.0 40.0 20.0 0.0 Control Plain water Ozone water Sonication 0.0 Control Plain water Ozone water Sonication Not stored 5-days Not stored 5-days

18.0 3.50 Soluble solids (%brix) 17.5 17.0 16.5 16.0 15.5 15.0 Resveratrol content (μg/g) 3.00 2.50 2.00 1.50 1.00 0.50 14.5 Control Plain water Ozone water Sonication 0.00 Control Plain water Ozone water Sonication Not stored 5-days Not stored 5-days Sphericity Weight(g) 1.2 12.0 1.0 10.0 0.8 8.0 0.6 6.0 0.4 4.0 0.2 2.0 0.0 CONTROL UV-A 40W UV-A 200W UV-C 40W UV-C 190W 0.0 CONTROL UV-A 40W UV-A 200W UV-C 40W UV-C 190W

L a 35.0 30.0 25.0 20.0 15.0 10.0 5.0 0.0 CONTROL UV-A 40W UV-A 200W UV-C 40W UV-C 190W 4.0 3.5 3.0 2.5 2.0 1.5 1.0 0.5 0.0 CONTROL UV-A 40W UV-A 200W UV-C 40W UV-C 190W b ΔE 0.0-0.5-1.0-1.5-2.0-2.5 CONTROL UV-A 40W UV-A 200W UV-C 40W UV-C 190W 40.00 35.00 30.00 25.00 20.00 15.00 10.00 5.00 0.00 CONTROL UV-A 40W UV-A 200W UV-C 40W UV-C 190W Deformation(mm) Rupture force(g) 3.0 2.5 2.0 1.5 1.0 0.5 0.0 CONTROL UV-A 40W UV-A 200W UV-C 40W UV-C 190W 80 70 60 50 40 30 20 10 0 CONTROL UV-A 40W UV-A 200W UV-C 40W UV-C 190W Sluble soilds(brix %) Moisture content(%) 25.0 100.0 20.0 80.0 15.0 60.0 10.0 40.0 5.0 20.0 0.0 CONTROL UV-A 40W UV-A 200W UV-C 40W UV-C 190W 0.0 CONTROL UV-A 40W UV-A 200W UV-C 40W UV-C 190W

Resvratrol content(ug/g) Resveratrol amplification ratio 7.0 6.00 6.0 5.0 4.0 3.0 2.0 1.0 5.00 4.00 3.00 2.00 1.00 0.0 CONTROL UV-A 40W UV-A 200W UV-C 40W UV-C 190W 0.00 CONTROL UV-A 40W UV-A 200W UV-C 40W UV-C 190W Sphericity Weight(g) 1.2 12.0 1.0 10.0 0.8 8.0 0.6 6.0 0.4 4.0 0.2 2.0 0.0 CONTROL UV-A 40W UV-A 200W UV-C 40W UV-C 190W 0.0 CONTROL UV-A 40W UV-A 200W UV-C 40W UV-C 190W L a 35.0 3.5 30.0 3.0 25.0 2.5 20.0 2.0 15.0 1.5 10.0 1.0 5.0 0.5 0.0 CONTROL UV-A 40W UV-A 200W UV-C 40W UV-C 190W 0.0 CONTROL UV-A 40W UV-A 200W UV-C 40W UV-C 190W

b ΔE 1.6 1.4 1.2 1.0 0.8 0.6 0.4 0.2 0.0 CONTROL UV-A 40W UV-A 200W UV-C 40W UV-C 190W 35.00 30.00 25.00 20.00 15.00 10.00 5.00 0.00 CONTROL UV-A 40W UV-A 200W UV-C 40W UV-C 190W Deformation(mm) Rupture force(g) 2.5 120 2.0 100 1.5 1.0 80 60 40 0.5 20 0.0 CONTROL UV-A 40W UV-A 200W UV-C 40W UV-C 190W 0 CONTROL UV-A 40W UV-A 200W UV-C 40W UV-C 190W Soluble solids(brix %) Moisture content(%) 20.0 100.0 15.0 80.0 10.0 60.0 40.0 5.0 20.0 0.0 CONTROL UV-A 40W UV-A 200W UV-C 40W UV-C 190W 0.0 CONTROL UV-A 40W UV-A 200W UV-C 40W UV-C 190W

Resvratrol content(ug/g) Resveratrol amplification ratio 6.0 5.00 5.0 4.0 3.0 2.0 4.00 3.00 2.00 1.0 1.00 0.0 CONTROL UV-A 40W UV-A 200W UV-C 40W UV-C 190W 0.00 CONTROL UV-A 40W UV-A 200W UV-C 40W UV-C 190W 국내산 거봉과 칠레산 거봉의 Resveratrol 함량 비교 resveratrol 함량(ug/g) 6 5 4 3 2 1 0 control 처리후 control UV -C 40 UV -C 190 칠레산 거봉(기계) 국내산 거봉(실험실) UV -A 40 UV -A 200

12 Resveratrol content (ug/g) 10 8 6 4 2 0 0 100 200 300 400 500 600 700 UV irradiation (mj/cm^2) Before Just after 1 day later 2 days later 3 days later 4 days later 5 days later 6 days later

12 Resveratrol content (ug/g) 10 8 6 4 2 0 0 100 200 300 400 500 600 700 UV irradiation (mj/cm^2) Before Just after 1 day later 2 days later 3 days later 4 days later 5 days later 6 days later

30 Resveratrol content (ug/g) 25 20 15 10 5 0 0 100 200 300 400 500 600 700 UV irradiation (mj/cm^2) Before 1 day later 2 day later 3 day later 4 day later 5 day later 6 day later

레스베라트롤 함량 (μg/g 건중량) 30 25 20 15 10 5 0 1.24 무처리 1.15 26.35 UV 처리 10.95 MBA 거봉

UV 살균실 UV LAMP 10 - 환봉 본체 단면도 구동부 단면도 10 12 11 12 반 사 판 STS304 2 11 케스터 지유형 4인치 구 매 품 4 8 텐숀 롤러 50 X 450L 구 매 품 2 6 베 어 링 UCFL205 구 매 품 2 5 자외선살균등 120W 구 매 품 9 3 송 풍 기 100W 구 매 품 6 2 구동모터 0.4KW X 1/60 구 매 품 1 1 본 체 460W X 3800L STS304 1 NO NAME SPEC MATERIAL Q'TY REMARK 포도광처리 장치 KIM 한식연-1 한 국 식 품 연 구 원 6 3 4 1 7 5 9 8 2

품종: MBA 레스베라트롤 함량 (μg/g 건중량) 35 30 25 20 15 10 5 0 2.86 무처리 30.56 UV 처리

µ

inhibition (%) 80.00 수퍼머루 체쳐지지 않은것 inhibition (%) 70.00 수퍼머루 체친것 70.00 60.00 60.00 50.00 40.00 30.00 500 μg/ml 250 μg/ml 125 μg/ml 62.5 μg/ml 31.25 μg/ml 50.00 40.00 30.00 20.00 500 μg/ml 250 μg/ml 125 μg/ml 62.5 μg/ml 31.25 μg/ml 20.00 10.00 10.00 0.00 control UV-A 40W UV-A 200W UV-C 40W UV-C 190W 0.00 control UV-A 40W UV-A 200W UV-C 40W UV-C 190W inhibition (%) 80.00 70.00 거봉 체쳐진것 inhibition (%) 80.00 거봉 체쳐지지 않은것 60.00 50.00 40.00 30.00 20.00 500 μg/ml 250 μg/ml 125 μg/ml 62.5 μg/ml 31.25 μg/ml 70.00 60.00 50.00 40.00 30.00 20.00 500 μg/ ml 250 μg/ ml 125 μg/ ml 62.5 μg/ ml 31.25 μg/ ml 10.00 10.00 0.00 control UV-A 40W UV-A 200W UV-C 40W UV-C 190W 0.00 control UV-A 40W UV-A 200W UV-C 40W UV-C 190W

3.0 Caspase-3 activity (fold) 2.5 2.0 1.5 1.0 0.5 0.0 Control CAM 1 um 1 2 3 4 Control : control CAM : camptothecin 1 M 1 : MBA CTL 400 g/ml 2 : CTL 400 g/ml 3 : MBA UV-A 200W 400 g/ml 4 : UV-A 200W 400 g/ml

이

1. Control 2. 3. Resveratrol 50mM 4. MBA control 5. MBA UV-A 200W 6. MBA UV-C 190W 7. 거봉 control 8. 거봉 UV-A 200W 9. 거봉 UV-C 190W

1. LPS (-) 2. LPS (+) 3. Resveratrol 50 mm 4. MBA control 9. 거봉 control 5. MBA UV-A 40W 10. 거봉 UV-A 40W 6. MBA UV-A 200W 11. 거봉 UV-A 200W 7. MBA UV-C 40W 12. 거봉 UV-C 40W 8. MBA UV-C 190W 13. 거봉 UV-C 190W

NaNO 2 25 20 15 10 5 LPS(-) LPS(+) control UV -A 40W UV -A 200W UV -C 40W UV -C 190W 0 LPS(-) LPS(+) 수퍼머루 체친것 수퍼머루 체쳐지지 않은것 거봉 체친것 거봉 체쳐지지 않은것