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38 계분 (%)
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54 a ATCC : American type Culture Collection, b IFO : Institute for fermentation, osaka, c MPNU : Mycological lab. Pusan National University d Mycelial growth was measured after 7days of incubation, 30 (Quericus aliena sawdust 70% + rice bran 30%)
55 70% + Rice bran 30% 70%+Rice bran 30% 70%+Rice bran 30% a Mycelial growth was measured after 7 days of incubation, Rrice bran 10% Gluten 10% Peptone 2% Yeast extract 2% Scallop extract 2% Beef extract 2% Chicken extract 2% Silk worm 2% Shrimp powder 2% Silk worm pupae pd.2% Dog food 10% Wheat flour 10% Corn flou r 10% Fresh soybean pd.10% IS P 2% Adlay powder 10% Potato powder 10% Sea lettuce powder 2% Molasses 2% Malt powder 10% Sikhae waste 10% CMM * Corn cobs 10% coconut palm 10% PS 5%+ Cs 5% Cotton seed 10% Alfalfa hay 10% CaCl2 0.2% CMC 10% Carrageenan 10% Alginic acid 10%
56 Contamination percentage (%) Rice bran 14 10% Gluten 18 10% Peptone 8 2% Silk worm 12 2% Shrimp 36 powder 2% Beef 12 extract 2% 38 ISP 2% Sea lettuce 28 powder 2% Malt powder CMM 100% peanut 44 hulls Silk worm 24 pupae Fig. 3. Test of contamination possibility for incubation of P.
57 Contamination percentage (%) h r 3h r 5h r 7h r 10h r Fig. 5. Test of contamination possibility on Condensed Mixed Medium(CMM)
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59 Beef 1,000g + Tap water 3,500ml Enzyme hydrolysis (50± 5, 120rpm, 3hr) Filteration(40mesh) Beef extract 3,000ml (7.1brix, dry weight 213g)
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75 Sodium phosphate, Dibasic A.blazei(cm) D.Microsporus(cm) Normal 0.1% 0.2% 0.3% 0.4% 0.5% 0.6% 0.7% 0.8% 0.9% 1% A.blazei D.Microsporus Na 2 HPO 4 concentration (%) 0
76 Potassium phosphate, Dibasic + Sodium phosphate, Dibasic A.blazei(cm) D.Microsporus(cm) Normal ⅰ ⅱ ⅲ ⅳ ⅴ ⅵ ⅶ mix number 0 ⅰ : Potassium phosphate, Dibasic 0.05% + Sodium phosphate, Dibasic 0.05% ⅱ: Potassium phosphate, Dibasic 0.1% + Sodium phosphate, Dibasic 0.1% ⅲ: Potassium phosphate, Dibasic 0.2% + Sodium phosphate, Dibasic 0.2% ⅳ: Potassium phosphate, Dibasic 0.3% + Sodium phosphate, Dibasic 0.2% ⅴ: Potassium phosphate, Dibasic 0.4% + Sodium phosphate, Dibasic 0.4% ⅵ: Potassium phosphate, Dibasic 0.5% + Sodium phosphate, Dibasic 0.3% ⅶ: Potassium phosphate, Dibasic 0.6% + Sodium phosphate, Dibasic 0.2% D.Microsporus
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83 고고분고고 (%) 자자자인인인인균균균 1:25 1:50 1:100 1:200 버버 (g): 버(ml)
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85 5 5 Yield of polysaccharides(%) 자자자인인인인균균균 Yield of polysaccharides(%) 자자자인인인인균균균 Extraction Time (h) Extraction termperature
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116 60 50 * 40 * NO (µm) PG (µg/ml) PB + PB * * * NO (µm) Control PG LPS
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119 Cell viability (% of control) LPS PG (µg/ml) Cytotoxicity to YAC-1 (% of control) L-NMMA + L-NMMA * Control PG LPS *
120 Medium CD80 CD86 MHC I MHC II PG LPS Medium CD80 CD86 MHC I MHC II PG LPS
121 IL-12 p40/p70-pe Medium PB 2.4% 5.5% 18.2% 14.3% + PG 26.7% 5.0% + LPS CD11c-FITC IL-12 p70 (pg/ml) Bioactive IL-12 production * - PB * + PB * - PG LPS Normal DC PG DC LPS DC
122 4 / Medium 37 / Medium 6.7% 42.6% UN CD11c-PE 7.6% 22.4% PG 6.3% 28.2% LPS Dextran-FITC
123 Medium PG Inguinal lymph nodes Medium Brachial lymph nodes PG Cervical lymph nodes LPS Spleen
124 18.2% PG CD80 CD86 MHC I MHC II PG + G 624/ / / / / / / /1394 IL-12 p40/p70-pe 5.6% PG + G PG + S 4.8% PG + S CD11c-FITC 120 kda- Medium Genistein Genistein + PG PG PKC activity (Percentage of basal PG PG+H-89 PG+S PMA 75 kda- 50 kda- 37 kda- 25 kda-
125 UN PG LPS (20 min) p-erk1/2 ERK1/2 p-p38 p38 p65
126 PG + PD PG + SB PG + PDTC CD86 MHC II IL-12p40/p70-PE PG PG + PD PG + SB PG + PDTC 28.8% 8.7% 11.3% 6.4% CD11c-FITC
127 Isotype (A) Agaricus blazei (B) Cordyceps millitaris CD80 CD86 MHC I MHC II CD80 CD86 MHC I MHC II Medium PS LPS
128 IL-12p70 (pg/ml) (A) Agaricus blazei * * IL-12p70 (pg/ml) (B) Cordyceps millitaris * * 0 Medium LPS PS 0 Medium LPS PS
129 (A) Agaricus blazei Medium PS (B) Cordyceps millitaris LPS Medium PS LPS
130 (A) Agaricus blazei (B) Cordyceps millitaris IL-2 (pg/ml) * * IL-2 (pg/ml) * * Medium LPS PS 0 Medium LPS PS
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137 beta-glucan contents(%) Fig. 73.Beta-glucan contents of Agaricus blazei Murill 10.8 Rice straw compost media 14.7 The root of arrowroot
138 Fig 74. Cordycepin contents of various length of fruitng body in same strain cordycepin contents (ppm) cm 5cm 10cm Length of fruiting body Fig. 75. Cordycepin contents in various strains Cordycepin contents(mg/100 fruiting body) 머쉬텍균주 1.24 건세농장균주 일조산업균주 청원농산균주
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MHC T cell Receptor Antigen Antigen-presenting cell Antigen-reactive T cell CD28 B7 Activation (proliferation Cytokine production Cytotoxicity) Antigen-presenting cell Antigen-reactive T cell Dormant State
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