-, BSF BSF. - BSF BSF ( ),,. BSF -,,,. - BSF, BSF -, rrna, BSF.

Size: px

Start display at page:

Download "-, BSF BSF. - BSF BSF ( ),,. BSF -,,,. - BSF, BSF -, rrna, BSF."

종환 최
6 years ago
Views:

1 , 2 4 Ⅰ,Ⅱ 2013,, -,. - Human Cell.. -,., Biosand Filter(BSF) - BSF Main Filtering (Biofilm) BSF, BSF ( ),.

2 -, BSF BSF. - BSF BSF ( ),,. BSF -,,,. - BSF, BSF -, rrna, BSF.

3 (Fig. 1, 2). Fig 1 Fig 2 -,.. - Methanol, MTT assay, 50% (Fig.3) Fig 3 Methanol Human-Cell

4 1 : Paper Disc Method - Paper Disc,. Spread Plate Culture Pour Plate Culture,, Spreading. 2 : MIC(Minimum Inhibitory Concentration) - Paper Disc MIC Test. MIC. Biosand Filter(BSF) -,,, BSF 93 99% 99.9%. Fig 4 BSF Fig 5 BSF -

5 . - 3m, KSCY., BSF, - target.,. BSF, - BSF,.,, BSF., BSF 70% BSF Filtering,, Filtering. - BSF, (Ceramic Filter), (Membrane Filter),, BSF.

6 BSF, - Biosand Filter, ( 60cm). 10T(1cm). Paper Disc Method,. Paper Disc Clear Zone Steel Ruler. BSF ,300,000 1,243,870 56,130 1,500,000 1,101, ,700 1,000,000 1,456, ,050 1,700,000 1,699, ,500,000 5,500,

7 /, Clean Bench Autoclave Speed Vacuum Concentrator Magnetic bar & Stirrer Biosand Filter & (Escherichia coli) (Staphylococcus aureus) 4 C, Colony,.,,,, LB(Luria Bertan) MSA(Mannitol Salt Agar) (Fig. 6, 7, 8, 9, 10, 11). 2 Biosand Filter(BSF) Table.1 LB(Luria Bertan) (g) Yeast Extract 5 1 Tryptone 10 2 NaCl 10 2 Agar Magnetic Bar, D.W 1l, Stirrer. Yeast Extract, NaCl, Tryptone, Agar 1 : 2 : 2 : 3 1

2 MSA(Mannitol Salt Agar) (g) Phenol Red 0.025 0.

5 1 Peptone 10 4 Mannitol 10 4 Sodium Chloride 75 30 Agar 15

8 Mixing., Autoclave 120 C, C, Petri dish 1/2, 3, 4 C. Table.2 MSA(Mannitol Salt Agar) (g) Phenol Red Beef Extract Peptone 10 4 Mannitol 10 4 Sodium Chloride Agar MSA 100 ml,, ph 7.2 ~ 7.6, Autoclave 120 C, C, 10%. Petri dish. 10% NaCl, MSA NaCl 9.5%,. Mannitol Acid Phenol Red. Fig 6 Mixing Fig 7 Stirring Fig 8 Autoclave

9 Fig 9 Fig 10 Fig 11 -,,,. Dring Oven 50 C 3, Ethanol(96.4%), 45 C 72,, 45 C 1. (Fig. 12, 13, 14, 15, 16, 17) Fig 12 Fig 13 Fig 14 Fig 15 Fig 16 Fig 17

: Paper Disc Method - Gram, MSA(Mannitol Salt Agar),

Clear Zone Disc (8mm) (Fig. 19. 19. 20).

10 : Paper Disc Method - Gram, MSA(Mannitol Salt Agar), Gram, LB(Luria Bertan) 30 μl Spreading,. Paper Disc( 8mm), 50 μl Loading. Ethanol(96.4%) 50 μl, 37 C 24 Incubating, Clear Zone. Clear Zone Disc (8mm) (Fig ). Fig 18 Fig 19 Loading Fig 20 Incubating 3. -,, 4 8 Paper Disc Method. - 5, 25 (Table.3). -,. 8

11 (Fig.21). - 8,,,. Table (mm) H2 H3 H3 H5 H5 H Paper Disc( 8mm) Clear Zone. Ethanol(96.4%). 0~0 H0. 1~5 H1. 5~10 H2. 10~15 H3. 15~20 H4. 20~25 H5. Fig 21 ( )

12 - 50%, 10% , 20, 30, 40, 50%,.22 30%. 2, 25~35% 1%., 32%, 1% 30% (Ethanol, 96.4%) ( ) (Table.4, Fig.22). 1L 400g. - Paper Disc,.. Table 4 10% 20% 30% 40% 50% (mm) H2 H2 H3 H3 H3 H

13 -,, 4 C. - 2, 20(5 ) (Fig.23) ,. 16 제조기간별항균감수성검사 C l e a r Z o n e 실험군 13 단위 : mm 2 주 4 주 6 주 8 주 10 주 12 주 14 주 16 주 18 주 20 주 제조시기 ( 2 주단위 )

14 BSF : -, Novosphingobium, Catellibacterium, Aeromonas, Leclercia Microbacterium S rrna. - BSF 18, Firmicutes Brevibacillus, Bacteroidetes Cloacibacterium, Streptomyces, Microbacterium, Arthrobacter, Sphingomonas, Bradyrhizobium, Klebsiella, Enterobacter, Pantoea S rrna. - Biosand Filter 9 Bacteroidetes Cloacibacterium, Streptomyces,,Arthrobacter 16S rrna 3 (Fig.24). - Biosand Filter. BSF 생물막의미생물변화 고리울천 BSF 생물막환삼덩굴추출액첨가생물막 미생물 기회성병원균 Fig 24 BSF

15 - Paper Disc 6mm. Paper Disc 50 μl Loading, Disc,. Disc 8mm. -, 2.,,. Biosand Filter, Biosand Filter &,. Biosand Filter, Biosand Filter. -,,,. -, Biosand Filter(BSF),., -,,

16 . -,.. -,, ,.,,. Biosand Filter(BSF), -, R&D,.,. -,, HPLC ( ). - Ethanol(94.6%), Water, Methanol.

17 - 16 rrna,, PCR( ),. 4. & Filter - ( ) : Biosand 2014 : 99 C ( ) 2014 R&E ( ) 2014 ( ) ( ) ( ) International Journal of Environmental Research and Public Health ( ) 2015 THE PATHFINDER (2015.9) ( ) Biosand Filter ( ) HPLC, PCR Biosand Filter(BSF)

18 5. 1, 2010,, 13 2, 2013,, 3, 2008,, ( ) 28 3, 1994,, 26 4, 2008,, 28 5, 2012, in vitro, 25 2, 2002,,, 6, 2014, (Humulus japonicus), 46, 2006, Ashobolt N.J, 2004, microbial contamination of drinking water and disease outcomes in developing regions. Toxicology, p Sianipar C.P.M.; Yudoko G.; Dowaki K.; Adhiutama A, 2005, Design methodology for appropriate technology: Engineering as if people mattered. Sustainability p Clifford M.J, 2005, Appropriate technology: The poetry of science. Sci. Christ. Belief, p Stauber C.E.; Elliott M.A.; Ortiz G.M.; DiGiano F.A.; Sobsey M.D. 2006, Charactersation of The Biosand Filter for E. coli Reductions from Housegold Drinking Water under Controlled Laboratory and Filed Use Conditions. Water Science & Technology, p. 1 7.

ca_02.hwp

ca_02.hwp SPSPSPSP SPSPSPS SPSPSP SPSPS SPSP SPS 공기청정기 항균필터 SPS - KACA009-139 한국공기청정협회 2002년 12월 31일 제정 2016년 -월 -일 개정예정 http://www.kaca.or.kr 1.1 적용범위 이 표준은 에어필터로 공기중의 입자제거 및 미생물 제균 성능을 지닌 필터에 대하여