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3 2018 한국육종학회 - 차세대 BG21 사업단 -GSP 사업단공동심포지엄 [ 주요일정 ] 2018 년 7 월 11 일 ( 수 ) 2018 년 7 월 12 일 ( 목 ) 개회식 Plenary Session 1 부 중식 Plenary Session 2 부 iii

4 분과별학술발표 그린바이오포럼 < 생명공학기술활용, 우리의선택은? > 한국육종학회정기총회 & 학회상시상 포스터발표 간친회 2018 년 7 월 13 일 ( 금 ) 사업단 / 산림분야학술발표 Plenary Session 3 부 우수발표상시상식및폐회식 2018 년 7 월 14 일 ( 토 ) Field Trip iv

5 2018 한국육종학회 - 차세대 BG21 사업단 -GSP 사업단공동심포지엄 [ 분과발표 ] 2018 년 7 월 12 일 ( 목 ) 분과발표 -1: Breeding for Productivity 분과발표 -2: Breeding for Biotic Stress v

6 분과발표 -2: Breeding for Biotic Stress 분과발표 -3: Breeding for Abiotic Stress 분과발표 -4: Breeding for Functional Crops vi

7 분과발표 -4: Breeding for Functional Crops Concurrent session : 생명공학기술활용, 우리의선택은? 그린바이오포럼주관 vii

8 2018 한국육종학회 - 차세대 BG21 사업단 -GSP 사업단공동심포지엄 [ 사업단발표 ] 2018 년 7 월 13 일 ( 금 ) Concurrent session-1: 차세대바이오그린 21 사업 (1) 식물분자육종사업단 / 농업생명공학연구단 시스템합성농생명공학사업단 Concurrent session-2: 차세대바이오그린 21 사업 (2) 농생물게놈활용연구사업단 - 유전체육종활성화를위한빅데이터플랫폼소개 - viii

9 2018 한국육종학회 - 차세대 BG21 사업단 -GSP 사업단공동심포지엄 [ 사업단발표 ] Concurrent session-3: Golden Seed Project (1) 채소종자사업단 원예종자사업단 Concurrent session-4: Golden Seed Project (2) Concurrent session-5: 산림육종 ix

10 2018 KSBS-BG21-GSP Joint Symposium 1 st day [July 11, 2018] 2 nd day [July 12, 2018] Registration << Plenary Session 1 >> Lunch << Plenary Session 2 >> Oral Presentation x

11 2018 KSBS-BG21-GSP Joint Symposium << Plenary Session 2 >> Poster Presentation Banquet 3 rd day [July 13, 2018] Concurrent Session << Plenary Session 3 >> Awards Ceremony & Closing Remark 4 th day [July 14, 2018] Field Trip xi

12 Contents Plenary Session SYMP-01 Crop conventional breeding: Achievements and outlook 3 Hee-Jong Koh SYMP-02 Using natural variation and CRISPR to understand and improve the plant immune system 3 Gregory B. Martin, Robyn Roberts, Ning Zhang, Samantha Mainiero, Adrian F. Powell, and Susan R. Strickler SYMP-03 Current Research & Development of Biotech Crops 4 Hee Young Park SYMP-04 SYMP-05 A genomics approach to understand the genetic complexity of genomic regions harboring the prolamin gene loci in hexaploid wheat 4 Yong Q. Gu, Naxin Huo, Susan B. Altenbach, Lingli Dong, Daoweng Wang, Mingcheng Luo, Jan Dvorak, Jong-Yeol Lee, and Toni Mohr Development and prevalence of molecular markers for the improvement of horticultural crops 5 Illsup Nou SYMP-06 Big data based systems and synthetic agrobiotechnology toward crop improvement 5 Dong-Yup Lee xii

13 공동심포지엄분과발표및구두발표 OA-01 Current status and its prospects of rice breeding in China 9 ZhongZe Piao OA-02 A genome wide association study of seed protein and oil in soybean 9 Sungwoo Lee, Kyujung Van, Mikyung Sung, Leah K. McHale, Randall Nelson, Jonathan LaMantia, M. A. Rouf Mian OA-03 Dynamic effects of silicon in crops under abiotic stress conditions 10 Yoonha Kim OA 정종민, 김석만, 정지웅, 김보경, 이점호 OA-05 Development and Characterization of japonica Rice with Diverse Panicle and Grain Shape 11 Hyun-Su Park, Man-Kee Baek, Choon-Song Kim, Jeong-Kwon Nam, Gun-Mi Lee, Seul-Gi Park, Chang-Min Lee, Young-Chan Cho OA-06 Soybean production and breeding in Vietnam 12 Le Duc Thao OB-01 OB-02 Isolation and validation of a candidate Rsv3 gene from a soybean genotype that confers strain-specific resistance to soybean mosaic virus 12 Phu-Tri Tran, Kristin Widyasari, Jang-Kyun Seo, Kook-Hyung Kim Development of SNP markers tightly linked to two QTLs responsible for bacterial wilt resistance in tomato 13 Boyoung Kim, Je Min Lee, In Sun Hwang, Hyung Jin Lee, Eunyoung Seo, Doil Choi, Chang-Sik Oh OB-03 QTL and GWAS Analysis of Phytophthora Resistance in Capsicum 13 Muhammad Irfan Siddique, Hea-Young Lee, Koeun Han, Jelli Venkatesh, Jin-Kyung Kwon, Byoung-Cheorl Kang xiii

14 OB-04 Transcriptome dynamics of cysteine protease-mediated response against Xanthomonas oryzae pv. oryzae race K3a in rice 14 Marjohn Nino, Franz M. Nogoy, Me-Sun Kim, Ju-Young Yang, Le Van Trang, Kwon-Kyoo Kang, Yong-Gu Cho OC-01 OC-02 OC-03 NO news is good news for plants - investigation of plant defense system under nitrosative stress and its application to crop 14 Byung-Wook Yun Towards breeding for multiple abiotic stress resistance in rice: an example using Pup1 and the others 15 Joong Hyoun Chin, Jae-Hyuk Han, Na-Hyun Shin, Soo-Cheul Yoo, Jae-Hoon Moon Screening and functional analysis of rice transcription factors involved in abiotic stress tolerance 15 Kiyoon Kang, Nam-Chon Paek OC-04 RNA-Seq transcriptome analysis of rice root genes in response to water deficiencies 16 Yo-Han Yoo, Anil Kumar N.C, Jong-Chan Park, Yun-Shil Gho, Sang-Won Lee, Gynheung An and Ki-Hong Jung OC-05 Analysis of agronomic traits using plant phenomics in rice 16 Song Lim Kim, Inchan Choi, Hyenso Ji, Hongseock Lee, Nyunhee Kim, Eungyeong Lee, JeongHo Baek, and Kyung-Hwan Kim OC-06 Probable L-ascorbate peroxidase 4 (APX4-P) controls flowering time and antioxidant activity in rice 17 Yun-A Jeon, Gynheung An, Lae-Hyeon Cho, Hyun-Sook Lee, Sun Ha Kim, Won-Yong Song, Sang-Nag Ahn OD 조준현, 송유천, 한상익, 고종민 OD-02 Flower color modification through reconstruction of flavonoid biosynthetic pathway 18 Sun-Hyung Lim OD-03 Development of poplar super clones 18 Jin-Seoung Cho, Min-Ha Kim, Hyung-Woo Jeon, Eung-Jun Park, Hyoshin Lee, Young-Im Choi Jae-Heung Ko xiv

15 OD-04 Comparative transcriptome analysis identified candidate genes involved in browning of mycelium in Lentinula edodes 19 Seung il Yoo, Hwayong Lee, Kesavan Markkandan, Suyun Moon, Junsu Ko, Hojin Ryu, Chang Pyo Hong OD-05 QTL mapping for agronomic traits by genotyping-by-sequencing in rice 19 Cheryl Adeva, Yun-A Jeon, Kyu-Chan Shim, Luong Ngoc Ha, Woo-Jin Kim, Jun-Hyun Cho, Sun Ha Kim, Yun Gyeong Lee, Hyun-Sook Lee, Changsoo Kim, and Sang-Nag Ahn OD-06 Diversity of chloroplast genome of Cynanchum wilfordii and development of species unique KASP markers for authentication of C.wilfordii and C. auriculatum 20 Sae Hyun Lee, Hong-Seob Yu, Ho Jun Joh, Hyun-Seung Park, Jee Young Park, Tae-Jin Yang xv

16 공동심포지엄포스터발표 PA-01 PA-02 PA-03 PA-04 Chromosomal karyotype with simple sequence repeats by fluorescence in situ hybridization in Korean wheat 23 Seong-Woo Cho, Eun-jin Cho, Changsoo Kim, Youn-Soo Choi, Chon-Sik Kang, Chul Soo Park Marker association analysis to improve tiller number in Korean wheats for wheat breeding 23 Seong-Woo Cho, Taek-Gyu Kang, Seong-Wook Kang, Jung-Min Park, Chon-Sik Kang, Chul Soo Park Marker association analysis to improve tiller number in double haploid population for wheat breeding 24 Seong-Woo Cho, Taek-Gyu Kang, Seong-Wook Kang, Jung-Min Park, Chon-Sik Kang, Chul Soo Park Marker association analysis to improve tiller number in F 8 population for wheat breeding 24 Seong-Woo Cho, Taek-Gyu Kang, Seong-Wook Kang, Jung-Min Park, Chon-Sik Kang, Chul Soo Park PA-05 Dynamics of small RNAome in Arabidopsis thaliana during breaking seed dormancy 25 Taewook Kim, Sang-Yoon Shin, Minsu Park, Jemin Kim and Chanseok Shin PA-06 PA-07 PA-08 Capillary electrophoresis-mass spectrometry-based on metabolome analysis in tomato germplasms 25 Vimalraj Mani, Soyoung Park, Joon-Soo Sim, Su-Yeon Kim, Jaeeun Song and Bum-Soo Hahn Construction of a Linkage map and mapping of QTLs related to agronomic traits in DH population of maize using simple sequence repeat markers 26 Jae-Keun Choi, Kyu Jin Sa, Dae Hyun Park, Su Eun Lim, Si-Hwan Ryu, Jong Yeol Park, Ki Jin Park, Hae-Ik Rhee, Ju Kyong Lee Genetic diversity analysis among accessions of Perilla crop using new development microsatellite markers 26 Kyu Jin Sa, Ik-Young Choi, Kyong-Cheul Park, Ju Kyong Lee PA 안억근, 원용재, 홍하철, 박향미, 정국현, 현웅조, 장재기, 이정희, 이점호, 이상복, 정응기, 서정필 PA-10 Overexpression of Brassica rapa GROWTH-REGULATING FACTOR genes in B. napus increases organ size by enhancing cell proliferation 27 Joon Ki Hong, Eun Jung Suh, Hye-Jin Yoon, Jihee Park, Yeon-Hee Lee xvi

17 PA-11 PA-12 QTL analysis of yield-related traits using high-resolution genetic map in rice (Oryza sativa L.) 28 Ye-Ji Lee, Eun-Ju Byeon, Kyeong-Seong Cheon, Hyeonso Ji, Gang-Seob Lee, Ung-Han Yoon, Nam-In Hyung, Tae-Ho Kim In vitro clonal propagation and cryopreservation of Korean Arbor-vitae (Thuja koraiensis Nakai) via somatic embryogenesis 28 Chang-Ho Ahn, Hyeong-Soo Park, Yong-Eui Choi PA 김성용, 김태균, 강형식, 홍순영, 송승운 PA 박노봉, 남민희, 김상열, 여운상, 이지윤, 권오덕, 박동수, 이종희, 조준현, 송유천 PA 구다은, 주혜준, 권용락 PA 구다은, 페레즈무카사, 조병관, 주혜준, 권용락 PA 원용재, 안억근, 정국현, 홍하철, 현웅조, 박향미, 윤미라, 이정희, 서정필, 정응기, 성낙식, 곽지은, 오세관 PA 이지윤, 조준현, 이종희, 오성환, 박노봉, 송유천, 박병명, 오인여, 박동수, 남민희, 오명규 PA-19 PA-20 PA-21 A study of the role of INFLORESCENCE DEFICIENT IN ABSCISSION gene expression in tomato 32 Junewoo Lee, Ronghui Yang, Sonia Philosoph-Hadas, Shimon Meir, Cai-Zhong Jiang, Caren Chang, Young-Hoon Park, Byoung-Cheorl Kang, Mark L, Tucker, and Joonyup Kim Genome-wide SNP selection associated with protein and oil content in core collection of wild soybean using the elastic-net method 33 Woon Ji Kim, Hokeun Sun, Tae-Hwan Jun, Man Soo Choi, Sungtaeg Kang, Soon-Chun Jeong, Jung-Kyung Moon, Bo-Keun Ha Genome-wide SNP selection associated with amino acid content in core collection of wild soybean using the elastic-net method 33 Woon Ji Kim, Hokeun Sun, Tae-Hwan Jun, Man Soo Choi, Sungtaeg Kang, Soon-Chun Jeong, Jung-Kyung Moon, Bo-Keun Ha PA 손범영, 백성범, 김정태, 이진석, 배환희, 고영삼, 김선림 xvii

18 PA-23 PA-24 PA-25 Genetic relationship of tropical region-bred temperate japonica rice plants and their grain yield variations in three different tropical environments 34 Jeom-Sig Lee, Jieun Kwak, Mi-Ra Yoon, Choon-Ki Lee, Eun-Yeong Sim, Namgeol Kim, Ha-Cheol Hong De novo assembly of complete chloroplast genome from Pinus densiflora using oxford nanopore and illumina MiSeq 35 Il Hwan Lee, Jinjoong Kim, Donghwan Shim Comparative transcriptome analysis of dwarf and normal soybean obtained from crossing of G. max and G. soja 35 Neha Roy Samir, Yong-Wook Ban, Hana Yu, Kyong Cheul Park and Ik-Young Choi PA-26 Effecs of different seeding methods on growth and yield of wheat 36 Young-Jin Kim, Kyeong-Min Kim, Kyeong-Hoon Kim, Dong-Jin Shin, Jin-Kyeong Cha, Jong-Min Ko PA-27 Map-based cloning of SPLIT-HULL (SPH) gene related to hull splitting in rice (Oryza sativa L.) 36 Gileung Lee, Jeonghwan Seo, Zhuo Jin, Im Ji Hwan, Hee-Jong Koh PA-28 Development of late flowering chinese cabbage using CRISPR system 37 Sun-geum Jeong, Eun Ju Lee, Jun Young Choi, Mi Jin Kim, Min Jung, Man-Sung Yang, Youn-Sung Kim PA 최창현, 강천식, 손재한, 김양길, 이수경, 박종호, 박태일, 윤영미, 김경호, 이점호 PA 강천식, 손재한, 정영근, 최창현, 김양길, 윤영미, 박종호, 양선목, 김경호, 박태일, 이점호 PA 강천식, 손재한, 정영근, 최창현, 김양길, 윤영미, 박종호, 양선목, 김경호, 박태일, 이점호 PA 강천식, 손재한, 정영근, 최창현, 김양길, 윤영미, 박종호, 양선목, 김경호, 박태일, 이점호 PA-33, 39 박종호, 박태일, 김양길, 윤영미, 강천식, 최창현, 손재한, 한옥규, 윤건식, 장지선, 배정숙, 송재기 PA 강천식, 손재한, 정영근, 최창현, 김양길, 윤영미, 박종호, 양선목, 김경호, 박태일, 이점호 PA 조지홍, 최장규, 박영은, 임주성, 조광수, 장동칠, 천충기, 이영규, 강용구, 김성용, 조만현 PA 김양길, 이미자, 김경호, 강천식, 박종호, 박태일, 윤영미, 한옥규, 최진경, 배정숙, 송재기, 손재한, 최창현, 이점호 xviii

19 PA 서정현, 한원영, 김현태, 강범규, 신상욱, 박장환, 김홍식, 오은영, 이병원, 윤홍태, 최만수, 이영훈, 곽도연 PA 손지영, 공도훈, 김은경, 박현근, 최성태, 안광환, 권진혁 PA 박기진, 박종열, 류시환, 최승출, 최재근, 남궁민, 최재근, 최준근 PA-40 Development of Tos17 insertion mutants from Korean domestic rice cultivars 43 Kyong Mi Jun, Joung Sug Kim, Songhwa Chae, Yoon-Mok Pahk, Gang-Seob Lee, Yeon-Ki Kim and Baek Hie Nahm PA 백만기, 박현수, 남정권, 조영찬, 김보경, 김기영, 김우재, 김정주, 고재권, 신운철, 강경호, 정지웅, 김춘송, 정종민, 이건미, 박슬기, 이영복, 이점호 PA-42 An ERF family gene, OsTF1, plays functional roles in the control of plant growth and grain size in rice 44 Sangkyu Park, Da-Hye Kim, Bora Park, Jong-Yeol Lee, Sun-Hyung Lim PA-43 SCAR 44 강석범, 문영일, 윤수현, 진성범 PA-44 Antioxidant activity of Korean black soybean (Glycine max. L.) landraces 45 Kyung Jun Lee, Sebastin Raveendar, Jung-Ro Lee, Myoung-Jae Shin, Gyu-Taek Cho, Kyung-Ho Ma, Gi-An Lee PA-45 Classification of Aegilops genus based on pollen morphology 45 Wang Xiaohan, Kyung Jun Lee, Sebastin Raveendar, Gi-An Lee, Jung-Ro Lee, Myoung-Jae Shin, Gyu-Taek Cho, Kyung-Ho Ma PA-46 Distribution of -tocopherol content in the Korean landrace black soybean 46 Ga-hee Kim, Kyung Jun Lee, Sebastin Raveendar, Gi-An Lee, Jung-Ro Lee, Myoung-Jae Shin, Gyu-Taek Cho, Yoon-Sup So, Kyung-Ho Ma PA-47 ICRISAT 46 백형진, 마경호, 윤문섭, 곽도연, 고지연, 최명은, 이경준 PA-48 Morphological characteristic of seed traits among the Vicia species 47 Sea-Hee Han, Kyung Jun Lee, Sebastin Raveendar, Gi-An Lee, Jung-Ro Lee, Myoung-Jae Shin, Gyu-Taek Cho, Kyung-Ho Ma xix

20 PA-49 PA-50 Application of AFLP and CAPS markers to screen somaclonal variations among diploid and autotetraploids of small watermelons 47 Phanna Phat, Kyung-Ho Ma, SeaHee Han, JaeJong Noh, Gi-An Lee, Kyu-Tae Cho, Kyung Jun Lee, and Ho-Jong Ju The complete chloroplast genome of Panicum sumatrense Roth ex Roem. & Schult. using illumina sequencing 48 Sebastin Raveendar, Gi-An Lee, Kyung Jun Lee, Myoung-Jae Shin, Jung-Ro Lee, Gyu-Taek Cho, Kyung-Ho Ma PA 박명렬, 서민정, 윤홍태 PA-52 shed- ph 49 박은준, 양은영, 문지혜, 이옥진, 채수영, 김상규, 김대현, 이금선, 김혜림, 박태성, 허온숙, 김옥례, 장길수, 박종숙 PA 박향미, 이정희, 원용재, 정국현, 안억근, 현웅조, 정응기 PA 류시환, 박종열, 최재근, 남궁민, 최승출, 김문종, 용우식, 윤석원, 남경남, 박기진, 최준근 PA-55 Development of CAPS marker for identification of Lentinula edodes cultivars Sanmaru 1ho and Sanmaru 2ho 50 Hwa-Yong Lee, Suyun Moon, Chang-Duck Koo, and Hojin Ryu PA 김춘송, 조영찬, 백만기, 박현수, 정종민, 남정권, 김보경, 이건미, 박슬기, 이창민 PA-57 Development and characterization of japonica rice line with long and spindle-shaped grain 51 Hyun-Su Park, Man-Kee Baek, Jeong-Kwon Nam, Woon-Cheol Shin, Gun-Mi Lee, Seul-Gi Park, Chang-Min Lee, Choon-Song Kim, Young-Chan Cho PA 박현수, 백만기, 김춘송, 남정권, 이건미, 박슬기, 이창민, 서정필, 조영찬 PA 박현수, 남정권, 조영찬, 김보경, 백만기, 고재권, 김기영, 신운철, 김우재, 김정주, 이점호, 고종철, 정종민, 강경호, 정지웅 PA 박슬기, 이건미, 이효정, 백만기, 박현수, 남정권, 김춘송, 김보경, 조영찬 xx

21 PA 하건수, 송윤호, 조영래, 오혜진, 조윤상, 이지우, 김상수 PA-62 Adaptability and clonal selection of poplar clones to combat desertification in arid aria of mongolia 54 Wonwoo Cho, Hyemin Lim, Jun-Won Kang, Hyunseok Lee, Sol-Ji Lee, Ganchudur Tsetsegmaa, Eunjeong Choi, Jiwon Han, Romika Chandra, Hoduck Kang PA 이지우, 김성용, 정재환, 이혜리, 하건수, 송윤호, 조윤상 PA-64 YR29169, A mid-maturing japonica rice elite line in tropical regions 55 Sumin Jo, Tae-Heon Kim, Jun-Hyeon Cho, Ji-Yoon Lee, Yeong-Ho Kwon, Saes-Beul Lee, Joo-Won Kang, You-Chun Song, Dong-Soo Park, Jong-Min Ko, Jong-Hee Lee PA-65 PA-66 SNP(Single Nucleotide Polymorphism) 55 조광수, 조지홍, 박영은, 임주성, 장동칠, 최장규 High-throughput SNP discovery by transcriptome sequencing to enhance the genomics-assisted breeding in Radish inbred lines 56 Abinaya Manivannan, Jinhee Kim, Hye-Eun Lee, Eun-Su Lee, Do-Sun Kim and Seung-Kook Choi PA 박영은, 조지홍, 이영규, 장동칠, 조광수, 임주성 PA-68 New genome wide SSR markers using high-throughput sequencing in button mushroom (Agaricus bisporus) 57 Hyejin An, Hwa-Yong Lee, Sebastin Raveendar, Tae-Young Heo, Yoon-Sup So and Jong-Wook Chung PA-69 SSR 57 안혜진, 권혁주, 정종욱 PA-70 SSR marker 58 방준형, 류호진, 정종욱 PA-71 New chloroplast microsatellite markers for identification of Glycyrrhiza species 58 Ji Seon Choi, Kyung Jun Lee, Sebastin Raveendar, Jinsu Gil, Yoon-Sup So and Jong-Wook Chung PA-72 New CAPS and HRM markers acquired from the chloroplast genome of wild licorice (Glycyrrhiza lepidota) 59 Ick Hyun Jo, Chi Eun Hong, Sebastin Raveendar, Kyong Hwan Bang and Jong-Wook Chung PA-73 Solidity 59 백정호, 이은경, 김년희, 김송림, 최인찬, 지현소, 최만수, 문중경, 김경환 xxi

22 PA-74 Protein sequence to vector strategy for ML application on proteins 60 Yang Jae Kang PA-75 The chloroplast genomes are variable in Prunus yedoensis 60 Eun Ju Cheong, Ik-Young Choi, Minho Na, Hwa Lee PA-76 Identification of biomass improved gibberellin biosynthesis related gene in rice 61 Won-Tae Yang, Ki-Deuk Bae PA 김년희, 이홍석, 김송림, 이은경, 백정호, 지현소, 최인찬, 박부희, 안혜련, 김경환 PA 최인찬, 김송림, 이홍석, 이은경, 김년희, 백정호, 지현소, 김경환 PA 이은경, 백정호, 김송림, 김년희, 최인찬, 최만수, 문중경, 지현소, 김경환 PA-80 Genetic and MutMap analysis of dense green leaf mutant lines of rice 63 Jun Oh, Hyun-Ju Kang, Hyo Ja Oh, Song Lim Kim, Inchan Choi, Jeongho Baek, Kyung-Hwan Kim, Hyeonso Ji PA 이홍석, 최인찬, 김년희, 김송림, 백정호, 김경환 PA-82 (Hovenia dulcis Thunb.) microsatellite 64 정지희, 류민하, 권용락, 송현진, 김호방 PA 박태일, 박종호, 김양길, 박형호, 한옥규, 우제훈, 홍기흥, 최규환, 강천식, 윤영미, 손재한, 최창현, 이점호 PA-84 Basic helix-loop-helix 65 최현모, 박단비, 배은경, 최영임 PA-85 SAP11 65 최지원, 최현모, 최영임, 이효신, 배은경 PA-86 A new early maturing and high yielding vegetable peanut variety "Sewon" 66 Suk-Bok Pae, Myung-Hee Lee, Eun-Young Oh, Sung-Up Kim, Jung-In Kim, Un-Sang Yeo, Ki-Won Oh, Do-Yeon Kwak, Yong-Chul Kim PA-87 Using genotyping-by-sequencing for revealing genetic relationship and diversity of sesame genotypes (Sesamum indicum L.) with black and white Seed Coat 66 Sovetgul Asekova, Sung Up Kim, Eun Young Oh, Krishnanand P. Kulkarni, Myoung Hee Lee, Jung In Kim, Suk Bok Pae, Do Yeon Kwak and Tae Joung Ha xxii

23 PA-88 PA-89 The SNP-based molecular diversity analysis of sesame genotypes and characterization of susceptible and resistant lines to Phytophthora nicotianae 67 Sovetgul Asekova, Sungup Kim, Eunyoung Oh, Myoung Hee Lee, JungIn Kim, Suk-Bok Pae, and Tae Joung Ha Development of infectious clones of Tomato yellow leaf curl Kanchanaburi virus (TYLCKaV) and Pepper yellow leaf curl Thailand virus (PYLCTHV) 67 Jongwook Ahn, Seula Choi, Min-Young Kang, Joung-Ho Lee, Jin-Kyung Kwon, and Byoung-Cheorl Kang PA-90 Identification of gene for gametophytic development in Arabidopsis 68 Saima Akhter, Elnura Torutaeva, Sung Aeong Oh, Soon Ki Park PA 최장규, 조지홍, 박영은, 임주성, 조광수, 장동칠, 천충기 PA-92 CRISPR/Cas9-mediated mutagenesis in Petunia hybrida protoplast system for Modification of Flower Color 69 Saminathan Subburaj, Luhua Tu, Ka-Youn Lee, Yong-Sam Jeon, Sangsu Bae, Geung-Joo Lee PA-93 Phylogenetic analysis of 22 Vigna species using chloroplast RNA-sequencing data 69 Jayern Lee, Sangrea Shim, Jungmin Ha, Suk-Ha Lee PA-94 Elucidation of senescence mechanism with T-DNA insertional mutant ck40 in rice 70 Trang Thi Do, Na-Hyun Shin, Maricar Ferrer Flores, Soo-Cheul Yoo PA-95 PA-96 PA-97 PA-98 Characterization and cytomorphology of F1 and F2 hybrid between transgenic Brassica napus and B. rapa 70 Soo-In Sohn, Young-Ju Oh, Ancheol Chang, Sun-Woo Oh, Gang-Seob Lee, Do Won Yun, Sung-Dug Oh Early stage assessment of sesame genotypes from worldwide collection for agro-morphological traits and genetic diversity 71 Yedomon Ange Bovys Zoclanclounon, Hong-Il Ahn, Ji-Nam Kang, Ung-Han Yoon, Tae-Ho Kim, Byoung-Ohg Ahn, Keunpyo Lee Comparison of the irradiation effects between proton beams and gamma rays on M1 seeds and seedling growth in rice for mutation breeding 71 Hong-Il Choi, Sung-Il Lee, Sung Min Han, Jae Wan Park, Sang Hoon Kim, Yeong Deuk Jo, Min Jeong Hong, Jin-Baek Kim, Si-Yong Kang Characteristic analysis of the CaCPR1 gene as a class of key enzyme that promote P450 enzyme reactions which related with productivity 72 Hyun min Kim, Ga-young Lee, Ah young Kim, Sang hoon Ma, Seo young Park, Mai Thanh Dat, Ju hui Do, Chul-ho Yun, Young hee Joung PA 권순욱, 박소연, 김동용 xxiii

24 PA-101 Pecos 73 이창민, 박현수, 백만기, 김춘송, 남정권, 이건미, 박슬기, 서정필, 조영찬 PA 김민주, 박재호, 윤수현, 진성범, 박석만 PA-104 Detected candidate gene related mesocotyl elongation using GWAS and haplotype analysis 75 So-Yeon Park, Heng Wang, Da-Eun Im, San Mar Lar, Seong-Gyu Jang, Sang-Hyeon Jin, Ja-Hong Lee, Soon-Wook Kwon PA 김현태, 강범규, 서정현, 이영훈, 이병원, 서민정, 고종민, 윤홍태, 신상욱, 김홍식, 곽도연 PA-106 An ancient regulatory module for tip growth in land plants 76 Youra Hwang, Hee-Seung Choi, Hyun-Min Cho, Hyung-Taeg Cho PA 송석보, 고지연, 우관식, 정태욱, 최명은, 김정인, 고종철, 문중경, 박장환, 최유미, 곽도연 PA 이형운, 남상식, 강용구, 양정욱, 유경단, 한선경, 고산, 이경보, 황엄지 PA 이덕렬, 조승현, 송영은, 권석주, 이인석, 이송이 PA 김성훈, 조규택, 마경호, 이정로, 이기안, 신명재, 세바스틴, 왕샤오한 PA-111 Haplotype analyze of the BADH1 gene and their association with salt tolerance in Rice 78 Yuan Cao, Sang-Ho Chu, Kyu-Won Kim, Yong-Jin Park PA-112 (IT ) 79 신명재, 이기안, 김성훈, 마경호, 이정로, 옥현충, 조규택 PA-113 Evolutionary studies on cultivated and wild rice using chloroplast genome sequencing 79 Lin Cheng, Kyu-Won Kim, Jungrye Nam, Yong-Jin Park PA-114 PA-115 Haplotype diversity of the rice bacterial blight resistance gene in 475 accessions of rice genetic resources 80 Jee-Su Kang, Jungrye Nam, Kyu-Won Kim, Yong-Jin Park Whole genome sequencing revealed a novel fragrance allele and development functional SNP marker for breeding of fragrant rice 80 Rungnapa Phitaktansakul, Sang-Ho Chu, Kyu-Won Kim, Young-Sang Lee, Yong-Jin Park xxiv

25 PA-116 Haplotype diversity of GBSSI in 475 accessions of rice genetic resources 81 Ji-Min Yoo, Sang-Ho Chu, Kyu-Won Kim, Yoo-Hyun Cho, Yong-Jin Park PA-117 Large-scale vector sequence sampling to develop DNA chips for GMO identification 81 Jungrye Nam, Kyu-Won Kim, Ji-Hye Hwang, Sook Kim, Hyun Namgung, Young-Chul Jeon, Gang-Seob Lee, Jung-Kyung Moon, Yong-Jin Park PA-118 Genetic diversity in 3,475 rice accessions of the Oryza genus 82 Kyu-Won Kim, Jungrye Nam, Yong-Jin Park PA-119 Development of candidate DNA markers for 833K rice integrated DNA array 83 Kyu-Won Kim, Jungrye Nam, Ji-Min Yoo, Sang-Ho Chu, Rungnapa Phitaktansakul, Jee-Su Kang, Myeong-Hyun Min, Lin Cheng, Yuan Cao, Sang-Beom Lee, Ji-Hye Hwang, Sook Kim, Hyun Namgung, Young-Chul Jeon, Soo-Cheul Yoo, Joo-hyun Lee, Soon-Wook Kwon, Joong-Hyoun Chin, Ki-Hong Jung, Young-Sang Lee, Nam-Chon Paek, Gang-Seob Lee, Yoo-Hyun Cho, Sang-Won Park, Il-Pyung Ahn, Hyeon-So Ji, Seung-Hyun Kim, Byeong-Chul Moon, Hee-Jong Koh, Jung-Kyung Moon, Ill-Min Chung, Yong-Jin Park PA 신동진, 김경훈, 김경민, 차진경, 김영진, 고종민 PA-121 Selection of indica rice line of high yield potential adaptable to tropical Southeast Asia Young-Chan Cho, Man-Ki Baek, Hyun-Su Park, Jong-Min Jeong, Chun-Song Kim, You-Chun Song, Jun-Hyun Cho, Ji-Yun Lee, Bo-Kyeong Kim, Jeom-Ho Lee 84 PA-122 햠 QTL mapping 85 조다님, 김민수, 하보근, 강성택, 송종태, 이정동 PA-123 PA-124 PA-125 The haplotype diversity of pivotal genes across 475 Korean rice core sets in a vitamin E biosynthesis pathway provided in the rice reactome database 85 Ji-Min Yoo, Sang-Ho Chu, Yong-Jin Park Productivity abilities of seed and cone in mating design conditions of Pinus densiflora for. multucaulis 86 Hyunseok Lee, Won-Woo Jo, Solji Lee, Hyemin Lim, Wi-Young Lee, Jun-Won Kang 86 이솔지, 이현석, 조원우, 강준원, 임혜민, 이위영, 김판기 PA-126 KASP 87 정영민, 이윤영, 이영실, 이하얀, 손미연, 지현소, 조영일 PA-127 Vernalization-related genes regulate development of wheat spike primordium 87 Cheol Won Lee, Yong Weon Seo xxv

26 PA 장성율, 이운석, 김형석 PA-129 The influence of modifiers genes on the stability of self-incompatibility and genetic architecture of Heterostyly and Homostyly in Buckwheat 88 Ju-Young Choi, Soo-Jeong Kwon, Ki-Hyun Kim, Seong-Woo Cho, Yong-Gu Cho, Y. Yasui and Sun-Hee Woo PB-01 PB-02 PB-03 PB-04 Sequence-based genotyping for marker discovery in Lettuce(Lactuca sativa L.) using GBS technology 89 Jin-Kee Jung, Eun-Jo Shim, Hyun-Jung Kim, Sujin Lee, Hye-Won Kim, Mi-Su An, Sun-Young Ko and Seung-In Yi Isolation and identification of the fungal leaf pathogens of mungbean (Vigna radiata (l.) R. Wilczek) leaf spot disease 89 Jun Hee Jung, Jungmin Ha, Moon Young Kim, Jung-Eun Kim, Hokyoung Son and Suk-Ha Lee Development of DNA markers for Slmlo1.1, a new mutant allele of the powdery mildew resistance gene SlMlo1 in tomato (Solanum lycopersicum) 90 Daun Kim, Byeogil Je, Byung Sup Kim, Hee-Jeong Jung, Ill-Sup Noua, Younghoon Park The Inquieta gene encoding a subunit of actin filament regulates trichome development in toamto 90 Na-Rae Jeong, In-Taek Hwang, Heejin Kim, Jae-In Chun, Jin-Ho Kang PB 안율균, 안영은, 홍규현, 이관호, 우영회, 김옥례, 이준구, 황인덕, 채수영 PB-06 Selection of novel source of rice stripe virus resistance 91 Sais-Beul Lee, Tae-Heon Kim, Joo-Won Kang, Su-Min Jo, Jun-Hyeon Cho, Ji-Yoon Lee, Jong-Hee Lee, Yeong-Ho Kwon, You-Chun Song, Jong-Min Ko, Dong-Soo Park PB-07 PB-08 Xanthomonas oryzae pv. oryzae triggers transcriptional activation of diverse defense-related genes in rice 92 Marjohn Nino, Franz M. Nogoy, Me-Sun Kim, Sothea Ouk, Ju-Young Choi, Yu-Jin Jung, Kwon-Kyoo Kang, Yong-Gu Cho Molecular mapping of Chili veinal mottle virus resistance genes in pepper using two different approaches 92 Joung-Ho Lee, Jongwook Ahn, Jin-Kyoung Kwon, and Byoung-Cheorl Kang xxvi

27 PB-09 Characterization and Isolation of a spotted leaf sheath Mutant with Early Senescence Involved in Defense Response in Rice 93 Dongryung Lee, Yoon Kyung Lee, Taejun Kim, Eunbyeol Koh, Hee-Jong Koh PB-10 Characterization of a new mutant affecting trichome development in tomato 93 Won-Ki Hong, Na-Rae Jeong, Heejin Kim, Jin-Ho Kang PB-11 QTL analysis of irregular cracking in soybean seed coats 94 Beom-Kyu Kang, Jeong-Hyun Seo, Hyun-Tae Kim, Hong-Sik Kim, Sang-Ouk Shin, Chang-Hwan Park, Do-Yeon Kwak PB-12 Characterization of mutants related to trichome morphology in tomato 94 Jae-In Chun, Heejin Kim, In-Taek Hwang, Na-Rae Jeong, Won-Ki Hong, Jin-Ho Kang PB-13 Nitric oxide increases ginsenosides accumulation by lactic acid bacteria elicitors in adventitious root cultures of Panax ginseng 95 Thanh-Tam Ho, Keon-Il Kim, So-Young Park PB 서민정, 김율호, 강인정, 이영훈, 이영기, 박명렬, 윤홍태 PB-15 PB-16 PB-17 Identification, classification, and expression analysis of the receptor-like protein family in tomato 96 Won-Hee Kang, Boseul Park, Seon-In Yeom Genome-wide identification and transcriptional co-regulation analysis of receptor-like protein genes in pepper (Capsicum annuum) 96 Won-Hee Kang, Namjin Koo, Yong-Min Kim, Junesung Lee, Seungill Kim, Doil Choi, and Seon-In Yeom Development and evaluation of clustered resistance gene analogs based markers linked to the resistance locus to Phytophthora capsici in hot Pepper 97 Nayoung Kim, Won-Hee Kang, Hakgi Jang, Kwanbeen Park, Hyunju Ha, Seon-In Yeom PB (IS592BB) 97 박현수, 남정권, 김우재, 백만기, 조영찬, 김보경, 김기영, 신운철, 김정주, 고재권, 이점호, 정지웅, 강경호, 김춘송, 정종민, 이건미, 박슬기, 이동규 PB 문지혜, 허윤찬, 박동금, 박태성, 양은영, 김수, 채수영 PB-20 New BB resistance gene confirmation by QTL fine mapping using 7K SNP-chip in bi-parental population 98 Suk-Man Kim, Rusell Reinke xxvii

28 PB-21 GWAS for detecting relative gene to apple blotch disease in apple 99 Jiheun Noh, Gi Hoon Kim, Jeong-Hee Kim, Dong-Hyuk Lee, Yun Su Do and Cheol Choi PB-22 MAB 99 강주원, 이지윤, 조준현, 권영호, 조수민, 박동수, 송유천, 이종희 PB-23 Genetic analysis of bakanae disease resistance of Samgwangbyeo 100 Hyun-Ju Kang, Jun Oh, Hyo Ja Oh, Song Lim Kim, Inchan Choi, Jeongho Baek, Kyung-Hwan Kim, Hyeonso Ji PB-24 Roles of OsWRKY67 in basal and XA21-mediated resistance in rice 100 Kieu Thi Xuan Vo, Chi-Yeol Kim, Dae-woo Lee, Seok-Hyun Choi, Suhyeon Shim, Jong-Seong Jeon PB-25 PB-26 Quantification of ingested plant subcellular fractions and dsrna by Frankliniella occidentalis for the establishment of RNAi-based control system 101 Min Ju Kim, Seung Hee Han, Kyungmun Kim, Sang Hyeon Kim and Si Hyeock Lee Genome-wide association analysis of flowering time genes with nested association mapping(nam) population in soybean 101 Kyung Hye Kim, Hong Min Koh, Ji-Min Kim, Taeklim Lee, Jinho Heo, Jiyeong Jung, Ju Seok Lee, Soon-Chun Jeong, Man Soo Choi, Jung-Kyung Moon, Bo-Keun Ha, Namshin Kim, Sungtaeg Kang PB-27 (UV-B) 102 고홍민, 김경혜, 김지민, 이택림, 허진호, 정지영, 강성택 PB-28 SNP 102 김경혜, 고홍민, 김지민, 이택림, 허진호, 강범규, 김성민, 한상훈, 이주석, 강성택 PB-29 Detection of the disease resistance genes using GWAS in grape vine 103 Hyun A Jang, Kyo-Sang Lee, Ha-Yeon Yoon, May Moe Oo, Tae-Seok Kwak, Mi-Reu Kim, Sang-Keun Oh PB-30 Genome-wide investigation and expression analysis of F-box genes in wheat development stages 103 Dae Yoen Kim, Min Jeong Hong, Yong Weon Seo PB-31 Functional analysis of plant transcription factor related to disease resistance 104 Hye Seon Kim, Kyong Sil Lee, Eun Hee Kim, Duk-Ju Hwang, Il-Pyung Ahn, Shin-Chul Bae, Sang Ryeol Park PB-33 Development InDel molecular markers for distinguishing between Korean cymbidium and Chinese cymbidium 105 So-Yeon Park, Su-Hyang Song, Heng Wang, Da-Eun Im, San Mar Lar, Seong-Gyu Jang, Sang-Heon Jin, Ja-Hong Lee, Jun-Myeong Lee, Dong-yong Kim, Soon-Wook Kwon xxviii

29 PC-01 PC-02 Genome-wide characterization of the Brassica rapa genes encoding serine/arginine (SR)-rich proteins: expression and alternative splicing events by abiotic stresses 105 Soo In Lee, Jin A Kim, Mi-Jeong Jeong Cloning and characterization of two phytochelatin synthases in rice (Oryza sativa cv. Milyang 117) that respond to cadmium stress 106 Hyeong Cheol Park, Jung Eun Hwang, Yun Jeong Kim, Myung Hwan Shin, and Woo Sik Chung PC-03 Analysis of salt-tolerant by M2 generation EMS variation-induced maize inbred lines 106 Dool Yi Kim, Kyung Hwa Kim, Man Soo Choi, Mi na Jin and Jae Buhm Chun PC-04 Saltol 107 김경화, 최만수, 전재범, 진민아, 안억근, 박향미, 김둘이 PC-05 Biological responses of cowpea plants after gamma-ray and proton-beam irradiation 107 Ryulyi Kang, Gyutae Kim, Woon Ji Kim, Si-Yong Kang, Dong-Kwan Kim, Bo-Keun Ha PC-06 Distribution of gene-based polymorphisms in tropical maize by using RNA sequencing 108 Kitae Song, Hyochul Kim, Kyung-Hee Kim, Jun-Cheol Moon, Jae Yoon Kim, Byung-Moo Lee PC-07 PC-08 PC-09 PC-10 PC-11 PC-12 Transcriptional network regualtion of BES1/BZR1-TPL-HDA19 in Brassinosteroid signaling pathway 108 Hyemin Kim, Hyeona Hwang and Hojin Ryu Physiological and molecular evaluation of Cheongho Byeo for salinity tolerance at the seedling stage 109 Ji Seon Song, Suyeon Kim, Mi Hyun Cho, Man-Kee Baek, Doolyi Kim, Beom Gi Kim, and In Sun Yoon Identification of OsRF1, a salt-responsive RING zinc-finger-encoding gene, conferring drought and salt tolerance in rice 109 Mi Hyun Cho, Suyeon Kim, Ji Seon Song, Beom Gi Kim, and In-Sun Yoon Ectopic expression of an ABA receptor, which functions specifically under high ABA concentrations, enhances drought tolerance in rice 110 Jin-Ae Kim, Nikita Bhatnagar, Myung Ki Min, Sujung Ra, In Sun Yoon, Young-Joo Seol, Beom-Gi Kim Overexpression of a member of OsDREB1subfamily, OsDREB1H confers cold stress tolerance in rice 110 Sujung Ra, Suk-Jun Moon, Jin-Ae Kim, Myung Ki Min, In Sun Yoon, Young-Joo Seol, Beom-Gi Kim Overexpression of PsGPD from Pleurotus sajor-caju enhances tolerance to salt stress in rice 111 So-Young Kim, Min Kang, Hye-min Lim, Seung Uk Ji, Gang-Seob Lee xxix

30 PC-13 Overexpression of BrTST53 Gene improves tolerance of rice plant to salt stress 111 Min Kang, So-Young Kim, A-Ram Kim, Seung Uk Ji, Gang-Seob Lee PC-14 Transcriptome analysis of pre-harvest sprouting (PHS) in Korean wheat (Triticum aestivum) 112 Jae Yoon Kim, Sang Yong Park, Kyeong Do Min, Chang-Ho Kim PC 현웅조, 원용재, 홍하철, 정국현, 안억근, 박향미, 윤미라, 이정희, 이상복, 신동범 PC-16 Arabidopsis NAC103 transcription factor is involved in the DNA damage response in sog1 mutant 113 Young Sam Go, Tae Ho Ryu, Seung Hee Choi, and Jin-Hong Kim PC-17 Ortholog gene of OsHCI1, Sorghum bicolor heat-induced RING finger E3 ligase 113 Dae Gyeom Oh and Cheol Seong Jang PC-18 PC-19 PC-20 Analysis of physiological response and protein expression in Korean F1 maize hybrids at flowering stage under water deficit stress 114 Hwan Hee Bae, Sang Gon Kim, Jin-Seok Lee, Jung-Tae Kim, Beom-Young Son, Young Sam Go, Sun-Lim Kim, Seong-Bum Baek Identifying salt stress response genes during germination stage in rice (Oryza sativa L.) using Genome-wide association study 114 Yoonjung Lee, Mijeong Kim, Taegyu Kim, Tae-Ho Ham and Joohyun Lee Evaluating the major agrinomic characters of four forage crops at Saemangeum-reclaimed land of Jellabuk-do Province 115 In-Sok Lee, Chan-Ho Kang, Jeong-Man Kim PC 김정주, 남정권, 정종민, 정지웅, 고재권, 김보경, 이점호 PC-22 Effect of salt stress on germination and seedling growth in soybean 116 Niaz Mohammad Rahimi, Jong-Tae Song, Jeong-Dong Lee PC-23, SPAD 116 김정태, 배환희, 이진석, 손범영, 고영삼, 김선림, 백성범 PC-24 qvdt11, a major QTL that positively regulates tillering in rice under drought stress condition in field 117 Tae-Heon Kim, Sumin Jo, Jun-Hyeon Cho, Jong-Hee Lee, You-Chun Song, Dong-Soo Park, Jong-Min Ko, Dongjin Shin xxx

31 PC-25 Genome-wide analysis of auxin efflux carrier protein family in rice reveals the close association with nitrogen stress response 117 Do-Young Bae, Yun-Shil Gho, Ki-Hong Jung PC-26 mirnaome analysis in flood-tolerant soybean Cheongja Green Jhang, Gyoungju Nah, Sukha Lee PC-27 PC-28 Increasing cold tolerance of transgenic Zoysia japonica by overexpression of a MYC type transcription factor ZjICE1 118 Zhi-Fang Zuo, Hana Jeong, Hyeon-Jin Sun, Mi-Young Park, Hong-Gyu Kang, Hyo-Yeon Lee Screening of drought- and flood-tolerant soybeans in core populations and EMS-treated 'Pungsannamul' mutant population 119 Cuc Thi Nguyen, Jagadeesh Sundaramoorthy, Gyu Tae Park, Jeong-Dong Lee, Jong Tae Song PC-29 Enhancement of soybean drought tolerance by genetic transformation 119 Jin Sol Park, Hye Jeong Kim, Hyun Suk Cho, Jae Seong Kim, Wan Woo Yeom, Jae Yong Han, Dae-Jin Yun, Young-Soo Chung PC-30 AN1/A20 zinc finger family 120 박단비, 최지원, 배은경, 최현모, 이효신 PC-31 PC-32 Soybean NAC transcription factors promote the lateral root formation and enhance drought and salt stress tolerance in overexpressed transgenic Arabidopsis plants 120 Xuefei Yang, Jungmin Ha, Suk-Ha Lee Analysis of QTL interaction for low-temperature germinability using progenies derived from an interspecific cross in rice 121 Kyu-Chan Shim, Sunha Kim, Hyun-Sook Lee, Yun-A Jeon, Luong Ngoc Ha, Cheryl Adeva, Woo-Jin Kim, Sang-Nag Ahn PC-33 Marker development of the genes/qtls related to biotic and abiotic stress tolerances 121 Je-Hun Moon, Jung-Woo Lee, Soo-Cheul Yoo PC-34 PC-35 PC-36 Mitogen Activated Protein Kinase (MAPK)-related genes that increase cadmium contents in rice 122 Sang-Beom Lee, Gyeong-Jin Kim, Yu-Ji Lee, Kyu-Won Kim, Sang-Ho Chu, Ji-Hyun Yoon, Buung Choi, Ji-Hyock Ryu, Byeong-Churl Moon, Yong-Jin Park, Sang-Won Park Characterization of BSP protein family from Triticum aestivum L. and its expression under the high temperature 122 Chan Seop Ko, Jin-Baek Kim, Min Jeong Hong, Kyeong Hoon Kim, Yong Weon Seo OsMDHAR3 gene affects salt stress tolerance and grain yield under natural field conditions 123 Young-Saeng Kim, Il-Sup Kim, Jin-Ju Kim, Seong-Im Park xxxi

32 PC-37 PC-38 PC-39 PC-40 Fine mapping and identification of candidate genes associated with low-temperature germinability using derived from a cross between Oryza sativa and Oryza rufipogon 123 Sun Ha Kim, Hyun-Sook Lee, Kyu-Chan Shim, Yun-A Jeon, Anh Quynh Le and Sang-Nag Ahn Changes of germination rate on seed germplasm of maize, sorghum, and cowpea after long-term conservation 124 Young-yi Lee, Hyung-jin Baek, Jung-yoon Yi, and Mun-seop Yoon Identification and marker development of Cucumber s chilling tolerance related genes by Next Generation Sequencing 124 Won-Kyung Lee, Jee Young Park, Sang-Choon Lee, Ho Jun Joh, Jaehyeon Jeon, Kihwan Song, Tae-Jin Yang Evaluation of anaerobic germinability and relative expression analysis of anaerobic-responsive genes in rice germplasm 125 Muhammad Rauf, Do Yoon Hyun, Sejong Oh, Myung-Chul Lee, Yu-Mi Choi, Sukyeung Lee PC 이다솜, 이진원, 윤상대, 소문수, 오성앵, 박순기 PC-42 Screening sorghum varieties for salt tolerance 126 MyeongEun Choe, Jee Yeon Ko, Seuk Bo Song, Do Yeon Kwak PC-43 CBF1 overexpression confers heat tolerance in Arabidopsis seedlings 126 Sang Dae Yun, Jin Won Lee, Da Som Lee, Sung Aeong Oh, Moon-Soo Soh, Soon Ki Park PC-44 GWAS and eqtl on preharvest sprouting characteristics of Asian cultivated rice 127 Myeong-Hyun Min, Sang-Ho Chu, Kyu-Won Kim, Yong-Jin Park PC-45 The development of transgenic rice pool over-expressing full length genes related to stress response in Oryza sativa 127 Hye-Jin Yoon, Seung-Bum Lee, and Yeon-Hee Lee PC-46 Variation in early plant height in wild and cultivated soybean ( ) 128 Thuy Thi Le, Minsu Kim, Rupesh Tayade, Niaz Mohammad Rahimi, Jong-Tae Song, Jeong-Dong Lee PC-47 Variation in early plant height in wild and cultivated soybean ( ) 128 Thuy Thi Le, Minsu Kim, Danim Jo, Rupesh Tayade, Niaz Mohammad Rahimi, Bo-Keun Ha, Jong-Tae Song, Jeong-Dong Lee PC-48 Differential protein expressions of soybean leaves under waterlogging stress at early vegetative stage 129 Jang-Hwan Yu, Hyeong-Jun Park, Swapan Kumar Roy, Soo-Jeong Kwon, Ju-Young Choi, Seong-Woo Cho, Hong-Sig Kim and Sun-Hee Woo xxxii

33 PC-49 PC-50 PC-51 PC-52 Proteome analysis of sesame leaves under waterlogging stress at an early vegetative stage 129 Hyun-Jin Jung, Sang-Heon Choi, Swapan Kumar Roy, Soo Jeong Kwon, Seong-Woo Cho, and Sun-Hee Woo Responses of leaf proteins in Azuki bean at early vegetative and reproductive stage to waterlogging stress 130 Hae-Ryong Jeong, Swapan Kumar Roy, Soo-Jeong Kwon, Ku-Hyun Kwon, Seong-Woo Cho, and Sun-Hee Woo Genome-wide identification and characterization of bzip transcription factor gene family in mungbean (Vigna radiata (l.) R. Wilczek) 130 Kang-Heum Cho, Jungmin Ha, Moon Young Kim, Sangrea Shim and Suk-Ha Lee Chromosome investigations on three tetraploid of Chrysanthemum species through FISH analysis 131 Franklin Hinosa Mancia, Ki-Byung Lim, Min Hee Ju, Hyo Bin Park, Lee Ye Jun, Hye Jin Seo, Min Hee Kim, Woo In Jung, Woo Chul Ju, Yoon-Jung Hwang PD-01 A rice B-box protein, OsBBX14, finely regulates anthocyanin biosynthesis in rice 131 Da-Hye Kim, SangKyu Park, Jong-Yeol Lee, Sun-Hyung Lim PD-02 Molecular characterization of transgenic plants using Next Generation Sequencing and confirmation of insert in breeding combined trait products using sequencing analysis 132 Andre Silvanovich, Carl Garnaat, Colton Kessenich, Qing Tian, Bradley Comstock, Marianne Malven, Will Urquhart, Hye Jung Hyun, Jonghyun Kwon PD-03 Genome-wide identification of RLK and RLP gene family inradish (Raphanus sativus L.) 132 Yinbo Ma, Xiaona Yu, Su Ryun Choi, Dong Hyun Kang, Lu Lu, Man-Sun Kim and Yong Pyo Lim PD-04 PD-05 Anthocyanin content and antioxidant activity in several varieties of Sorghum bicolor (L.) Moench 133 Sang Chul Choi, Yun Gyeong Lee, Yu-Na Kang, Kyeong Min Kim, Changsoo Kim Physicochemical properties and eating quality of cooked rice in low amylose content under freezing storage 133 Eun Ji Lee, Jun Hyeon Cho, Ji Yoon Lee, Ju Won Kang, Young Ho Kwon, Soo Min Cho, Jong Hee Lee, Dong Soo Park, You Chun Song, Jong Min Ko PD-06 TALEN-based gene editing to produce herbicide-resistance bar knockout rice lines 134 Yang Qin, Ye-Jin Jang, Kong-Sik Shin, Tae-Sung Park, Myung-Ho Lim xxxiii

34 PD-07 Biochemical analysis of the role of leucine-rich repeat receptor-like kinases and the carboxyterminus of receptor kinases in regulating kinase activity in arabidopsis thaliana and brassica oleracea 134 Eun-Seok Oh and Man-Ho Oh PD 안억근, 현웅조, 정국현, 원용재, 홍하철, 박향미, 장재기, 이정희, 이점호, 성낙식, 서정필, 오세관, 윤미라 PD-09 Study about gene flow and stability assessment in GM rice 135 Yoon Hee Jang, Do Hyeon Gwon, Ju Hun Noh, Je Dong Kim, Kyung-Min Kim PD-10 PD-11 PD-12 PD-13 PD-14 PD-15 Evaluation of role of charismata mutase in aromatic amino acid and secondary metabolites biosynthesis by using yeast functional expression system 136 Rahmatullah Jan, Kyung-Min Kim Construction of genetic map by EST-SSR markers and QTL analysis of major agronomic characters in hexaploid oat (Avena sativa L.) 136 Leitao Pedro Isabel, Rahmatullah Jan, Kyung-Min Kim Evaluation of East Asian oat (Avena sativa L.) genetic resources based on major nutritional ingredients and agricultural traits 137 Sukyeung Lee, Yu-Mi Choi, Do yoon Hyun, Myung-chul Lee, Sejong Oh, Onsook Hur, Hocheol Ko, and Gyu Taek Cho User-friendly common platform using genotype, phenotype and chemotype for managing tartary buckwheat genetic resources 137 Su-Jeong Kim, Hwang-Bae Sohn, Su-Young Hong, Bon-Cheol Koo and Yul-Ho Kim Molecular footprints of adaptation and high flavonol content in tartary buckwheat revealed by a draft genome analysis 138 Yul-Ho Kim, Su-Jeong Kim, Hwang-Bae Sohn, Su-Young Hong and Bon-Cheol Koo Inheritance of fertility restoration of male-sterility conferred by cytotype y and identification of instability of male fertility phenotypes in onion (Allium cepa L.) 138 B. Kim, C. Kim and S. Kim PD-16 Compositional variability in diverse maize hybrids (Literature review) 139 Mia Park, Gregory B. Tilton, Erin Bell PD-17 Evaluation of gene flow from Bt transgenic rice to non-gm rice 139 Jiyoung Kim, Doh-Won Yun, Soo-In Sohn, Gang-Seob Lee, SongJae Ok, Ancheol Chang, Sung-Dug Oh PD-18 CEPR1 receptor kinase and gene expression in regulating carbon/nitrogen balance in Arabidopsis thaliana 140 Eun-Seok Oh and Man-Ho Oh xxxiv

35 PD-19 Selection of New Soybean Strain with ti and rs2 recessive allele 140 Sang-Woo Choi, Jun-Hyun Park, Jong-Il Chung PD-20 PD-21 Development of New Soybean Strain with Large Seed Size and ti and lox recessive allele 141 Sang-Woo Choi, Jun-Hyun Park, Jong-Il Chung Isoegomaketone-related genes revealed by RNA-Seq in mutant cultivar of Perilla frutescens var. crispa 141 Soon-Jae Kwon, Min-Kyu Lee, Dong-Gun Kim, Jung Min Kim, Min Jeong Hong, Bo Mi Nam, Chang Hyun Jin, Hong-Il Choi, Bo-Keun Ha, Jin-Baek Kim PD-22 Variation block browser for functional crops 142 Kesavan Markkandan, Jinuk Bhak, Dong Woo Lee PD-23 PD-24 Development of kompetitive allele specific PCR (KASP) markers in Dendrobium genotypes derived from mutation breeding 142 Jaihyunk Ryu, Woon Ji Kim, Kyung-Won Kang, Sang Hoon Kim, Si-Yong Kang, Jeong-Hee Lee, Bo-Keun Ha Development and validation of kompetitive allele specific PCR (KASP) assay in Rubus mutant genotypes 143 Jaihyunk Ryu, Woon Ji Kim, Kang-Seop Lee, Han-Jig Jo, Ee-Youb Kim, Si-Yong Kang, Jeong-Hee Lee, Bo-Keun Ha PD 고세리, 장지영, 이주영, 최규환 PD-26 PD-27 PD-28 TRAP markers can be used to determine mutation frequencies induced by gamma radiation in faba bean (Vicia faba L.) 144 Min-Kyu Lee, Dong-Gun Kim, Jung Min Kim, Bo-Keun Ha, Min Jeong Hong, Jin-Baek Kim, Si-Yong Kang, and Soon-Jae Kwon A WUSCHEL homeobox transcription factor, OsWOX13, enhances drought tolerance and triggers early flowering in rice 144 Joung Sug Kim, Songhwa Chae, Kyong Mi Jun, Gang-Seob Lee, Dong-Eun Kim, Jong-Joo Cheong, Sang Ik Song, Baek Hie Nahm, and Yeon-Ki Kim Identification of QTLs associate with flowering time using a GBS-SNP-based high-density map in Perilla 145 Yun-Joo Kang, Bo-Mi Lee, Jae-Eun Oh, Moon Nam, Ki-Won Oh, Myoung-Hee Lee, Tae-Ho Kim, Sung-Hwan Jo, and Jeong-Hee Lee PD-29 Evaluation of Genetic Diversity in Korean rice varieties as revealed by SSR markers 145 Me-Sun Kim, Jae-Young Song, Sothea Ouk, Franz M. Nogoy, Marjohn Nino, Kwon-Kyoo Kang, Sun-Hee Woo, Yong-Gu Cho xxxv

36 PD-30 Identification of SNP related to leaf-angle traits using a genome-wide association study in rice (Oryza sativa L.) 146 Me-Sun Kim, Sothea Ouk, Le Van Trang, Franz M. Nogoy, Marjohn Nino, Ju-Young Yang, Kwon-Kyoo Kang, Yong-Gu Cho PD-31 callus isoflavone 146 김용호, 최도진, 이주원, 마무현, 이유정, 조민식, 이주희, 김홍식 PD-32 Phytosterol 147 김선림, 김미정, 정건호, 이유영, 이진석, 손범영, 김정태, 배환희, 고영삼, 백성범 PD 최재근, 박종열, 장은하, 류시환, 박기진, 남궁민, 윤석원, 용우식, 최준근 PD-34 PD-35 PD-36 PD-37 Characterization of complete chloroplast genomes of Adenophora triphylla and Codonopsis lanceolata 148 Junki Lee, Hae Rim Park, So Yeon Kim, JongSung Lim, Green Jhang, Hyang Sook Chun, Kisung Kwon, Youngho Koh, Tae Sun Kang and Gyoungju Nah Characterization of complete chloroplast genomes of Hemerocallis fulva and Veratrum japonicum 148 Junki Lee, So Yeon Kim, JongSung Lim, So Yeon Kang, Hyang Sook Chun, Kisung Kwon, Youngho Koh, Tae Sun Kang and Gyoungju Nah Analysis of transferability of kenaf EST-SSR marker to other Hibiscus genus and their use in studying genetic diversity 149 Jung Min Kim, Jaihyunk Ryu, Min-Kyu Lee, Dong-Gun Kim, Min Jeong Hong, Jin-Baek Kim, Si-Yong Kang, Bo-Keun Ha, Joon-Woo Ahn, Soon-JaeK won Characterization of complete chloroplast genomes of Kalopanax septemlobus and Zanthoxylum ailanthoides 149 Jin-hyuk Kim, Junki Lee, Eun-Kyoung Kim, Minjung Kim, Sanghee Um, Soon-Sung Choi, Hwa-Young An, Hyang Sook Chun and Gyoungju Nah PD-38 Bx7 150 손재한, 강천식, 최창현, 김양길, 박종호, 박태일, 윤영미, 정영근, 김경호, 이점호 PD-39 PD-40 Application of TRAP markers to genetic diversity and relationship in soybean mutant lines 150 Dong-Gun Kim, Young Eun Jang, Min-Kyu Lee, Jung Min Kim, Min Jeong Hong, Jin-Baek Kim, Si-Yong Kang, Chang-Hyu Bae, Joon-Woo Ahn, Soon-Jae Kwon Population genetic structure and genetic diversity of peanut (Arachis hypogaea L.) collections 151 Kunyan Zou, Daewoong Lee, Nayoung Kwon, Tae-Hwan Jun xxxvi

37 PD-41 Roles of perilla heading date gene, pfhd3a, in flowering time 151 Hee Rang Yun, Jee Hye Kim, Hae Eun Kim and Jae Bok Heo PD-42 Plastidal ribosomal protein caused albino, seedling death phenotype in rice 152 Jinwon Lee, Gynheung An, Soon Ki Park PD-43 Plastidal ribosomal protein caused albino, seedling death phenotype in rice 152 Jinwon Lee, Gynheung An, Soon Ki Park PD-44 Utilization of Mutator transposable elements for waxy and common maize breeding program 153 Rahul Vasudeo Ramekar, Youn Sung Kim, Kyong Cheul Park and Ju Kyong Lee PD-45 Genetic mapping and characterization of a stunted growth mutant in rice 153 Hye-Kyung Son, Kishor Doddanakatte Shivaramegowda, Somyung Lee, You Seok Kang, Hee-Jong Koh PD-46 PD-47 PD-48 PD-49 Comprehensive transcriptome analysis of Ligularia fischeri to identify isoforms and biosynthesis genes associated with medicinal components 154 Rahul Vasudeo Ramekar, Yong Bog Kim, Byong Gon Choi, Sun Gang Choi, Ik Young Choi and Kyong Cheul Park Understanding the sensory characteristics of aromatic rice breeding line using an electronic nose 154 Song-Yee Lee, Deok-Ryeol Lee, Seung-Hyun Cho, So-Ra Choi, Eun-Ju Song, Young-Eun Song, Hyun-Ah Han, So-Hee Shin Compositional analysis of wheat LMW-GS using aroona near-isogenic lines and a set of standard cultivars by 2-DGE, MS/MS and RP-HPLC 155 Su-Jin Choi, You-Ran Jang, Susan B. Altenbach, Min-Ki Lee, Sun-Hyung Lim, Jong-Yeol Lee Development of wheat transformation methods by advanced particle bombardment in a Korean wheat cultivars Keumkang 155 Jin Sun Kim, Su-Jin Choi, You-Ran Jang, Sun-Hyung Lim, Jong-Yeol Lee PD 김경민, 김경훈, 신동진, 차진경, 김영진, 고종민 PD-51 The effect of the chemical components and glyoxylate cycle-related gene expression on sprouting speed of soybean seed 156 Beom-Kyu Kang, Jeong-Hyun Seo, Eun-Young Oh, Hyun-Tae Kim, Hong-Sik Kim, Sang-Ouk Shin, Chang-Hwan Park, Do-Yeon Kwak PD-52 Identify component difference of tea cultivars using fourier transform infrared(ft-ir) 157 So-Jin Lee, Yong-Hee Kwon, Chun-Hwan Kim, Chan-Kyu Lim, Seung-Yeob Song, Doo-Gyung Moon xxxvii

38 PD-53 Glucoraphanin 157 김영천, 차아라, 이상협 PD-54 MBW complex mediates the transcriptional regulation of anthocyanin biosynthesis in rice 158 Da-Hye Kim, Bora Park, SangKyu Park, Jong-Yeol Lee, Sun-Hyung Lim PD-55 Redesign of starch biosynthetic pathway in rice by genome editing toward human diets 158 Hyo-Ju Lee, Ye Rim Lee, Yeo Jin Jung, Jong Hee Kim, Dong Hyun Kim, Jae Uk Lee, Hee Kyong Kim, Chang Sun Sin, Ki Hong Nam, Jeong Sun Park, Yu Jin Jung, Kwon Kyoo Kang PD-56 PD-57 Korean soybean (Glycine max) core collection: Identification of genetic diversity, morphological traits, population structure, and genome-wide association study by Axiom 180k SoyaSNP Genotyping Array 159 Namhee Jeong, Jaebuhm Chun, Dool-Yi Kim, Mina Jin, Jung-Kyung Moon, Man Soo Choi Transcriptome analysis using Iso-Seq provides a comprehensive view of fruit development in Schisandra chinensis 159 Dong Jin Lee, Chang-Kug Kim, Yi Lee, Chang Pyo Hong PD-58 A blue single freesia (Freesia hybrida Hort.) Blue Angel 160 Youn Jung Choi, Young Ran Lee, Yun Im Kang PD-59 A pink double freesia (Freesia hybrida Hort.) Pink Bubble 160 Youn Jung Choi, Young Ran Lee, Yun Im Kang PD-60 Polymorphic analysis of chloroplast genomes and 45S nrdnas reveals genetic diversity of Perilla species 161 Seon-Hwa Bae, Kyeong-Seong Cheon, Ye-Ji Lee, Hong-Il Ahn, Myoung-Hee Lee, Jeong-Hee Lee, Ung-Han Yoon, Jundae Lee, Tae-Ho Kim PD-61 Comparison of assembly protocol for Brassica rapa genome 161 Sin-Gi Park, Kesavan Markkandan, In-Gang Shin, Boram Choi, Chang Pyo Hong, Jung Sun Kim PD 김경훈, 차진경, 신동진, 김경민, 김영진, 현종내, 고종민 PD 이진석, 손범영, 김정태, 배환희, 백성범, 정태욱, 김병주 PD 하건수, 송윤호, 조영래, 오혜진, 조윤상, 이지우, 김상수, 송원종, 강철홍 xxxviii

39 PD-65 PD-66 Induced coiled branch (cbr) mutant in Arabidopsis by overexpression of a novel E3 ubiquitin ligase gene 163 Gyu Tae Park, Jagadeesh Sundaramoorthy, Hak Soo Seo, Jong Tae Song Development of a DNA barcode database for scientific authentication of raw materials for food safety: Current status and recent progress 164 Chi-hwan Kim, Junki Lee, Eunkyoung Jun, Yoojin Kim, Hyang Sook Chun and Gyoungju Nah PD 남궁민, 류시환, 최재근, 박종열, 박기진, 최승출, 김문종, 용우식, 남경남, 윤석원, 최준근 PD-68 Metabolic regulation for high production of syringin in Arabidopsis thaliana 165 Tackmin Kwon and Jaesung Nam PD-69 Identification of a new Sg-9 gene responsible for the DDMP-saponin biosynthesis in soybean 165 Jagadeesh Sundaramoorthy, Gyu Tae Park, Seokhyung Lee, Jeong-Dong Lee, Hak Soo Seo and Jong Tae Song PD-70 Expression of F-box proteins and their interacting proteins in wheat grain development 166 Min Jeong Hong, Dae Yeon Kim, Hong-Il Choi, Yeong Deuk Jo, Sang Hoon Kim, Soon-Jae Kwon, Joon-Woo Ahn, Yong Weon Seo, Jin-Beak Kim PD-71 Comparison of agricultural and physicochemical traits from tropical and subtropical legume crops 166 Yu Mi Choi, Do Yoon Hyun, Sukyeung Lee, Myung-Chul Lee, Sejong Oh, Hocheol Ko, On-Sook Hur, Munsup Yoon, Gyu Taek Cho PD-72 Overexpression of IbMYB1a gene in soybean increased high content of anthocyanin 167 Wan Woo Yeam, Hye Jeong Kim, Hyun Suk Cho, Jin Sol Park, Jae Yong Han, Cha Young Kim, Young-Soo Chung PD-73 PD-74 PD-75 PD-76 Development of molecular markers for discrimination between Ligularia fischeri and Caltha palustris using real-time PCR assay 167 Geum Sol Kim, Cheol Seong Jang OsSTK1enhances the intrinsic GTPase activity of OsNug2 through binding and phosphorylating its serine Chong Chen and Jae Bok Heo Variety discrimination of Fuji and its somatic mutation cultivar by InDel and AS-PCR primer using re-sequencing 168 Ji Yul Lee, Gi hoon Kim, Cheol Choi Distribution of capsaicinoid contents in pepper core collection understanding genetic association 169 Ju-Pyo Hong, Hea-Young Lee, Na Young Ro, Jin-Kyung Kwon and Byoung-Cheorl Kang xxxix

40 PD-77 The complete chloroplast genomes of six Ipomoea species and indel marker development for the discrimination of authentic pharbitidis semen (seeds of I.nil or I. purpurea) 169 Inkyu Park, Sungyu Yang, Wook Jin Kim, Pureum Noh, Hyun Oh Lee, Byeong Cheol Moon PD-78 Comparison of sesame seed qualities grown in field and in the greenhouse 170 Sungup Kim, Sovetgul Asekova, Eunyoung Oh, Jung In Kim, Myoung Hee Lee, Suk-Bok Pae, Tae Jung Ha, Do-Yeon Kwak PD-79 Development of web-based database for phenotype data management 170 Sung Jin Choi, Junkyoung Choe, Jae-eun Oh, Sung-Hwan Jo PD-80 PD-81 PD-82 Anti-oxidant, phenolic/flavonoid compounds, anti-elastase and anti-collagenase activities of extracts from Citrus unshiu at various stages of fruit development and tissues 171 Chang-Ho Eun, In-Jung Kim Differential expression analysis of RNA-seq with repect to capsaicinoid biosynthesis in the pericarp tissue of Capsisum chinense 171 Minjeong Park, Dogyeong Lee Joung-Ho Lee, Koeun Han, Byoung-Cheorl Kang Gene expression analysis varying apple peel color and pattern between Fuji and Benishogun 172 Hye Jeong Cho, Yu Jin Kim, Gi Hoon Kim, and Cheol Choi PD-83 Development of SNP marker associated with fruit traits using GWAS in apple 172 A Reum Han, Hyun Wook Shin, Gi Hoon Kim, and Cheol Choi PD-84 A Comparison of two de novo GBS pipelines for a large genome 173 Tae-Ho Lee, Jae-Hyeon Oh, Do-Wan Kim, Dong-Jun Lee, Byung-Ohg Ahn PD-85 PD-86 PD-87 Comparative analysis of synteny between plants to improve the quality of a genome sequences which is a base of modern breeding technologies 173 Tae-Ho Lee, Cho-Yil Cho, Jae-Hyeon Oh, Do-Wan Kim, Byung-Ohg Ahn Maturation rate of inflorescence stem determines the direction of stem growth in Solanaceae 174 Seunghye Park, Jung Heo, Min-Sung Kang, Soon Ju Park Assessment of potential environmental risks of transgene flow in smallholder farming systems in Asia: Brassica napus as a case study in Korea 174 Chuan-Jie Zhang, Min Jung Yook, Hae-Rim Park, Do-Soon Kim PD 김홍식, 김현태, 강범규, 서정현, 신상욱, 박장환, 곽도연 xl

41 PD-89 Construction of linkage maps in octoploid strawberry based on SNPs discovered by GBS method 175 Minjeong Park, Ju-pyo Hong, Jinhee Kim, Do-Sun Kim, Sun-yi Lee, Heayoung Lee, Koeun Han, Byoung-Cheorl Kang PD Do Yoon Hyun, Muhammad Rauf, Sejong Oh, Myung-Chul Lee, Yu-Mi Choi, Sukyeung Lee PD-91 flo14(t) 176 조준현, 강주원, 이지윤, 조수민, 권영호, 이샛별, 김태헌, 송유천, 박동수, 고종민, 이종희 PD-92 Genetic analysis of Purple pericarp (Prp) and Purple Leaf (Pl) traits of rice 177 Kyung Eun Lee, Sang Gu Kang PD-93 PD-94 PD-95 Identification and application of genome-wide SNPs using genotyping by sequencing (GBS) of 119 Panax ginseng accessions 177 Yeeun Jang, Woojong Jang, Hyun-Jin Koo, Hyunjo Koo, Vo Ngoc Linh Giang, Min Jung Kim, and Tae-Jin Yang Genetic variation of Major Carotenoids and fruit characteristics of 250 tomato germplasm 178 On-Sook Hur, Na-Young Ro, Jung-Sook Sung, Jae Eun Lee, Ho-sun Lee, Sok Young Lee, Awraris Derbie Assefa, Ju-Hee Rhee Search for clues to delaying tomato fruit ripening by sound wave through transcriptome analysis 178 Mi Jeong Jeong, Joo Yeol Kim, Soo In Lee, and Jin A Kim PD-96 Development of SNP array in single copy genes of Brassica oleraceae for MAB 179 Seung Woo Jin, Yeeun Jang, Sae Hyun Lee, Tae-Jin Yang PD 우관수, 심동환, 강승미, 안지영, 이제완, 홍경낙 Concurrent session OE-01 Deciphering the complex wheat gliadin proteins: basic study to application for development of allergen-reduced wheat 183 Jong-Yeol Lee, You-Ran Jang, Jin Sun Kim, Susan B. Altenbach, Sun-Hyung Lim xli

42 PE-01 PE-02 PE-03 PE-04 A novel RING type E3 ligase, CaASRF1, positively regulates drought tolerance via modulation of CaAIBZ1 stability in Capsicum annuum 184 Hyunhee Joo, Chae Woo Lim, Sung Chul Lee Late embryogenesis abundant protein, CaLEA5, positively regulates the drought tolerance and ABA signaling in Capsicum annuum 184 Junsub Lim, Chae Woo Lim, Sung Chul Lee A DEAD-box RNA helicase, RH8, is critical for regulation of ABA signaling and the drought stress response via inhibition of PP2CA activity 185 Woonhee Baek, Hyunhee Joo, Chae Woo Lim, Sung Chul Lee Transcriptome analysis of dwarf soybean suggests the association of carbon metabolism with dwarf phenotype 185 Taewook Kim, Jemin Kim, Minsu Park and Chanseok Shin PE-05 HCRs, anti-crossover proteins restrict crossover number in Arabidopsis 186 Juhyun Kim, Jaeil Kim, Hanul Kim, Eun-Jung Kim, Jihye Park, Annhee Im, and Kyuha Choi PE-06 Characterizing substrate specificity of guanosine deaminase from Arabidopsis thaliana 186 Sunyoung Yang and Sangkee Rhee PE-07 A study of allelic diversity underlying flowering-time adaptation in soybean cultivars using a variation block analysis 187 Hwang-Bae Sohn, Su-Jeong Kim, Su-Young Hong, Bon-Cheol Koo and Yul-Ho Kim PE-08 Role of chloroplast dynamics in plant innate immunity 187 Seungmee Jung, Jongchan Woo, Jeffery L. Caplan, Doil Choi, Savithramma P. Dinesh-Kumar, Eunsook Park PE-09 PE-10 PE-11 Plant pathogen effectors suppresses reactive oxygen species signaling networks in plants 188 Nam-Soo Jwa Potyviral CP-interacting proteins facilitate potato virus X infectivity by interacting with viral protein and RNAs. 188 Yeonhwa Jo, Jung Min Yoon, Kook-Hyung Kim and Hoseong Choi Homeobox transcription factor OsZHD2 promotes lateral root growth in rice by inducing ethylene and auxin biosynthesis 189 Jinmi Yoon, Lae-Hyeon Cho, Wenzhu Yang, Yunfei Wu, Woo-Jong Hong, Charlotte Bureau, Soo Jin Wi, Tao Zhang, Rongchen Wang, Ki-Hong Jung, Ky Young Park, Christophe Périn, Yunde Zhao, and Gynheung An PE-12 Regulation of chloroplast development by alternative splicing 189 Sangyool Lee, Sun Hyun Chang and Geupil Jang xlii

43 PE 고세리, 장지영, 이주영, 최규환 PE-14 Effector-assisted breeding methodology for bacterial wilt resistance in pepper 190 Boyoung Kim, Ankita Pandey, Hayoung Moon, Kee Hoon Sohn, and Cécile Segonzac PE-15 Identification of MRFs as an unusual type of translation regulators that are involved in plant mrna translation under low energy conditions 191 Du-Hwa Lee, Seung Jun Park, Chang Sook Ahn, and Hyun-Sook Pai PE-16 Functional characterization of chloroplast-targeted RbgA GTPase in higher plants 191 Young Jeon and Hyun-Sook Pai PE-17 PE-18 PE-19 PE-20 PE-21 PE-22 PE-23 Interspecific comparison of Angelica species using chloroplast indel markers developed from Angelica gigas Naki. 192 Jinsu Gil, Jaebok Lee, Sang Ik Park, So Hyeon Park, Kyoungmin Lee, Sung Cheol Koo, Ho Bang Kim, Yi Lee Functional properties of an alternative, tissue-specific promoter for rice NADPH-dependent dihydroflavonol reductase 192 Hye-Jung Lee, Joon ki Kim, Yu-Jin Jung, Kwon-Kyoo Kang, Ku-Hyun Kwon, Sang-Heon Choi, Michael Kovach, Susan McCouch, Yong-Gu Cho Brassinosteroids-controlled local auxin homeostasis is essential for xylem differentiation and wood formation in tomato 193 JinSu Lee Transcriptome analysis and development of EST-SSR markers from Codonopsis lanceolata 193 Jinsu Gil, Jaebok Lee, Sang Ik Park, So Hyeon Park, Kyoungmin Lee, Sung Cheol Koo, Ho Bang Kim, Yi Lee Identification of a novel seed specific glycine rich protein Dor1, which regulates seed dormancy in rice 194 Suyeon Kim, Sun Mi Huh, Hay Ju Han, Myung Hee Nam, Ju Hee Jung, Gang Seob Lee, Yong Sic Hwang, Beom Gi Kim and In Sun Yoon Systemic network analysis to gain candidate genes regulating seed storage proteins in rice 194 Kyoungwon Cho, Oksoo Han Post-translational modifications of E3 SUMO ligase AtSIZ1 are specifically regulated by heat and drought stresses 195 Yuna Kang, Hak Soo Seo xliii

44 PE-24 AGI(a-glucosidase inhibitor) 195 박도이, 양은영, 조명철, 채수영, 사무엘티라훈, 이준대 PE-25 Investigation of bacterial wilt pathogen effector diversity and interactions with host plants 196 Hayoung Moon, Maxim Prokchorchik, Ankita Pandey, Gayoung Jung, Honour C. McCann, Hongsik Won, Kwangsoo Cho, and Kee Hoon Sohn PE-26 Study on OsGT1 (Oryza sativa Grassy Tiller1) determining branching in rice 196 Chang-deok Han, Vikranth Kumar PE-27 Association between sequence variants in panicle development genes and the number of spikelets per panicle in rice 197 Su Jang, Choonseok Lee, Jin seok Moon, Mikyong Kang, Hee-Jong Koh PE-28 Rapid identification of HMW-GS in common wheat varieties by MALDI-TOF-MS 197 You-Ran Jang, Joo Ohk Sohn, Su-Jin Choi, Sun-Hyung Lim, Jong-Yeol Lee PE-29 PE-30 PE-31 PE-32 PE-33 The rice Rolled fine striped encodes CHD3/Mi-2 chromatin remodeling factor and is involved in scavenging reactive oxygen species during leaf development 198 Nam-Chon Paek, Kiyoon Kang EMF1 acts as a transcriptional repressor of VIN3, a gene required for sensing long-term winter cold for flowering 198 Jina Bae and Ilha Lee Molecular approach to develop novel plantsoptimizing the shade avoidance syndrome (SAS) 199 Yoon-Sun Hur, Sunghan Kim and Choong-Ill Cheon Diversity of chloroplast genome and development of SSR markers for breeding of Peucedanum japonicum 199 Ho Jun Joh, Hong-Seob Yu, Hyeonah Shim, Hyun-Seung Park, Sae Hyun Lee, Shin-Jae Kang, Tae-Jin Yang Physiological and biochemical conditions for discrimination between haploid and diploid maize kernels 200 강유나, Jong Tae Song, Si Wan Ryu, Hak Soo Seo PE-34 Pun1 homologs 200 김영천, 한지훈, 이상협 PE-35 Investigation of the in vitro effects of total extracts from pepper on amyloid production and aggregation 201 김영천, 이주영, 이상협 xliv

45 PE-36 PE-37 Investigation of alpha-glucosidase inhibitory activity in different pepper cultivars (Capsicum annuum L.) 201 김영천, 최다은, 이상협 Two circadian rhythm regulators, CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY) recognize long-term cold to induce VERNALIZATION INSENSITIVE 3 (VIN3) for flowering in Arabidopsis 202 Jinseul Kyung, Myeongjune Jeon and Ilha Lee PE-38 Modification of seed colors via LDOX gene editing by CRISPR/Cas9 system in rice 202 Hyo-Ju Lee, Ye Rim Lee, Hee Kyung Kim, Yeo Jin Jung, Sangsu Bae, Ji Hyeon Yu, Kwon Kyoo Kang, Yu Jin Jung PE-39 Genome-wide screening of abiotic stress-responsive long noncoding RNAs in rice 203 Nuri Oh, Yong-Jin Shin, Jimin Lee, Jaeho Kim, Pil Joong Chung and Choonkyun Jung PE-40 PE-41 PE-42 Stable recombinant protein expression in pepper using a broad bean wilt virus 2-based vector system 203 Bo-ram Choi, Soo-Jung Han, Siwon Choi, Kyeong-Jae Heo, Myung-Hwi Kim, and Jang-Kyun Seo Comparative analysis on transcriptome and metabolome between insect-resistant wild potato S. berthaultii and insect-susceptible potato S. tuberosum cv. Sumi to identify genes involved in insect-resistance 204 In-Taek Hwang, Na-Rae Jeong, Heejin Kim, Jin-Ho Kang Comparative gene expression analysis of seed development in waxy and dent maize (Zea mays) 204 Kyu Jin Sa, Ik-Young Choi, Kyong-Cheul Park, Ju Kyong Lee PE-43 Identification of candidate genes conferring resistance to foxglove aphid in soybean 205 Ju Seok Lee, Kyung Hae-Kim, Ji-Min Kim, Juhyun Im, and Sungtaeg Kang PE-44 PE-45 PE-46 Characterization of volatiles and phytonutrients in grains of advanced breeding lines to develop a new fragrant rice variety with superior nutritional value 205 Young-Sang Lee, Kyu-Won Kim, Yoo-Hyun Cho, Yong-Jin Park Comprehensive analysis of R Gene composition in a Ctv locus conferring citrus tristeza virus resistance from the genetic resources of citrus and its relatives 206 Jiyeon Jeong, Jin-Kyu Woo, Young Chul Park, Sukman Park, Su-Hyun Yun, Yi Lee, Gun-Hyoung Cho, Kwan Jeong Song, Ho Bang Kim Detection of SNPs among 13 Korean japonica rice varieties using genome sequencing data for SNP marker development 206 Hyeonso Ji, Jun Oh, Hyun-Ju Kang, Hyo Ja Oh, Song Lim Kim, Inchan Choi, Jeongho Baek, In Sun Yoon, Yong-Jae Won, Kyung-Hwan Kim xlv

46 PE-47 PE-48 PE-49 PE-50 Identification of structural and regulatory genes involved in anthocyanin biosynthesis that confers the flower color of chrysanthemum 207 Bo-Ra Park, Da-Hye Kim, Sangkyu Park, Jong-Yeol Lee, Sun-Hyung Lim Genomic structure and genome wide association study for seed weight using Korean soybean landrace and cultivar 207 Taeyoung Lee, Suk-Ha Lee Development of drought tolerant temperate rice varieties utilizing Pup1 and drought QTLs 208 Jae-Hyuk Han, Na-Hyun Shin, Je-Hun Moon, Soo-Cheul Yoo, Joong Hyoun Chin Identification of key plastidic phosphoglucomutase and ADP-glucose pyrophosphorylase isoforms essential for successful fertilization in rice 208 Sang-Kyu Lee, Seok-Hyun Choi, Suhyeon Shim, and Jong-Seong Jeon PE-51 Optimization of TRV mediated CRISPR/Cas9 genome editing in Tomato 209 Yoo-Jung Yoon, Jelli Venkatesh, Jin-Kyung Kwon, Byoung-Cheorl Kang PE-52 Changes in crop productivity induced by new sp mutant alleles 209 Min-Sung Kang, Jung Heo, Sukgui Oh, Jong Hyang Bae, Soon Ju Park PE-53 PE-54 PE-55 PE-56 PE-57 Characterization of the microtubule-associated RING finger protein 2 (OsMAR2), acts as a negative regulator in responses to salt stress in Arabidopsis 210 Ju Hee Kim and Cheol Seong Jang * Analysis of Anthocyanins, Chlorophyll and Carotenoid compounds during Seed Development in wheat (Triticum aestivum L.) 210 Paulina Calderon Flores, Yong Weon Seo Seed coat color segregation of an F3 (Yellow x Deep Purple), genetic analysis and temperature effects in wheat (Triticum aestivum L.) 211 Paulina Calderon Flores, Yong Weon Seo Study of gene expression and interacting proteins of wheat MAP Kinase family under cold treatment 211 Woo Joo Jung, Yong Weon Seo Identification of expression levels of arsenic-related genes and genome-wide transcriptome profiling of genes associated with arsenic in arsenic-tolerant rice mutant 212 Sang Cheul Lee, Cheol Seong Jang PE 안상낙, 김선하, 이현숙, 전윤아, Adeva Cheryl, 심규찬, Luong Nogc Ha, 김우진 xlvi

47 PE-59 PE-60 Identification and functional analysis of ASR genes under drought stress in Brachypodium distachyon L. 213 Jin Seok Yoon and Yong Weon Seo A genome-wide association study of biomass productivity in core collection of Miscanthus 213 Min Jung Yook, Yeon-Ho Park, Soo-Hyun Lim, Do-Soon Kim PE-61 Anti-osteoporosis effect of T99 soybean with high isoflavone content 214 Jiwon Kim, Donggyu Lee, JaeEun Lee, Youjin Lee, Hyeah Yu, Jiwon Jung and Namjoo Kang PE-62 Development of Miscold, a new cold-tolerant Miscanthus variety 214 Soo-Hyun Lim, Min Jung Yook, Yeon-Ho Park, Jin-Won Kim, Do-Soon Kim PE-63 Establishment of double-haploid breeding system to produce Korean wheat lines with extra-strong gluten and wheat-leymus addition lines 215 Seong-Woo Cho, Taek-Gyu Kang, Seong-Wook Kang, Eun-jin Cho, Chul Soo Park PE-64 KASP 215 이종희, 지현소, 강주원, 이지윤, 조수민, 권영호, 이샛별, 김태헌, 송유천, 박동수, 고종민 PE-65 PE-66 Development of efficient plant regeneration systems in chrysanthemum Ohblang and Baekgang 216 Yeo Jin Youn, Boram Beak, Seoyun Yu, Nam-In Hyung Development of a new S locus haplotyping system based on three tightly linked genes in the S locus controlling self-incompatibility in radish (Raphanus sativus L.) 216 Dong-Seon Kim, Sunggil Kim PE 도재왕, 윤재복, 이강현 PE-68 The Effects of Keunnunjami embryo on lipid meta metabolism 217 Mi Young Kang, Soo Im Chunga, Su Jin Nam, Ji E Liang, Jing Wen Ma PE-69 PE-70 The bean pod mottle virus silencing vector triggers acute response in Rsv resistance gene carrying soybean cultivars. 218 Mazen Alazem, Kristin Widyasari, Phu Tri Tran, and Kook-Hyung Kim Light inhibits COP1-mediated degradation of ICE transcription factors to induce stomatal development in Arabidopsis 218 Chungmo-Park xlvii

48 OF-01 Databases for metabolomics-assisted life science 221 Nozomu Sakurai PF-01 PF-02 Ubiquitin-specific protease, CaDUB1, positively regulates ABA signaling and drought tolerance in Capsicum annuum 221 Soongon Jung, Chae Woo Lim, Sung Chul Lee The function of the alpha 1,3-fucosyltransferase gene on anther and pollen development in rice (Oryza sativa) 222 Soyoung Park, Su-Yeon Kim, Vimalraj Mani, Jaeeun Song, Seong-Ryong Kim and Bum-Soo Hahn PF 박수철, 김수민, 임수미 PF-04 Ethephon application increased mineral uptake and development of adventitious roots of soybean (Glycine max L) under waterlogging condition 223 Yeon-Gyeong Park, Jeong-Dong Lee, In-Jung Lee, Yoonha Kim PF 홍준기, 서은정, 김경환, 이연희 PF 박지희, 홍준기, 정황원, 서은정, 이연희, 한정헌 PF-07 Characteristics of plant regenerated through anther culture using GM rice 224 Eun Gyeong Kim, Yoon Hee Jang, Joung Suk Choi, Kyung-Min Kim PF-08 PF-09 PF-10 QTL analysis with optical coherence tomography data to bacterial leaf blight in rice (Oryza sativa L.) 225 Xiao-Xuan Du, Sungwook Kim, Hyeree Kim, Mansik Jeon, Byoung-Ju Yun, Kyung-Min Kim Gravistimulation on different orientations changes the accumulation pattern of OsPIN genes encoding auxin efflux facilitator in the endodermis of transition zone in Oryza sativa seedlings 225 Muhammad Farooq, Kyung-Min Kim Development of performing new forms of rice (Oryza sativa L.) related to yield and ecology through SNDH population (Double Haploid) by using QTL analysis 226 Dany Resolus, Kyung-Min Kim PF-11 (GM) ( ) 226 김종미 xlviii

49 PF-12 PF-13 PF-14 Functional analysis of Oryza sativa LATE ELONGATED HYPOCOTYL (OsLHY) in controlling of seed development in rice 227 Jeong-Hyun Lim, Sang-Ji Lee, Byoung-Doo Lee, and Nam-Chon Paek Functional analysis of Oryza sativa Cryptochrome-Interacting Basic-Helix- Loop-Helix1 (OsCIB1) in controlling of leaf angle and grain size in rice 227 Hyoseob Seo, Byoung-Doo Lee, and Nam-Chon Paek The F-box protein FKF1 inhibits dimerization of COP1 in the control of photoperiodic flowering 228 Byoung-Doo Lee and Nam-Chon Paek PF-15 Development of transgenic rice producing dammarenediol-ii of ginseng 228 Jin-Suk Kim, Ga-Hyeon Kim, Eui-Shik Rha, Doh-Won Yun, Yong-Eui Choi, So-Hyeon Baek PF-16 PF-17 A N molecular sensor system: a breeding technique for development of high NUE rice under low N conditions 229 Dong-Keun Lee, Seowon Choi, Jae Sung Shim, Sung Hwan Kim, Hye In Jeong, Chang Mi Kim, Se Nam Lee, and Ju-Kon Kim Seralini's 2012 publication on long term animal study of GMO corn and its effect on the GMO risk communication in Korea 229 Jin Joo Choi, DongSub Kim, Jang Ryol Liu, Kyu Hang Kyung PF-18 PAPs-mediated chloroplast development in rice 230 Sangyool Lee, Sun Hyun Chang, Taeyoung Um and Geupil Jang PF-19 Optimization of virus induced gene silencing in different petunia cultivars using pepper phytoene desaturase (PDS) gene 230 Hyun Young Sing, Chang Kil Kim PF 고세리, 장지영, 이주영, 최규환 PF 장안철, 이범규, 곽영신, 김지영 PF-22 MULTISEEDED affects sorghum grain yield regulating at pedicellate spikelet fertility 232 Young Koung Lee, Yinping Jiao, Zhanguo Xin and Doreen Ware PF-23 Genome-wide analysis of long non-coding RNAs in radish (Raphanus sativus) 232 Su Hyun Park, Seunghoon Baek, Ara Cho, Jeong-Hwan Mun, Sang-Bong Choi PF 윤도원, 오성덕, 손수인, 장안철, 백소현, 최용의 xlix

50 PF 윤도원, 오성덕, 손수인, 장안철, 이효연, 서석철, 선현진 PF-26 Development of transgenic alfalfa having increased biomass and reduced lignin content 234 Yun Young Kim and Jeong Sheop Shin PF-27 Chemical composition and nutritions properties of soybean cultivars cultivated in the different locations 234 Seon-Woo Oh, So-Young Lee, Hwi-Young Park, Soo-Yun Park, Sang-Gu Lee, Seong-Kon Lee, Young-Soo Chung, Hyun-Suk Cho PF-28 Plasticity between PTI and ETI through Nonsense-mediated mrna decay 235 Gagan Kumar Panigrahi, Ki Hun Shin, Annapurna Sahoo, Kyung Man Kim, Sang Hyon Kim PF 박정호, 박지영, 엄민식, 김혜진, 김창기 PF-30 RNAi 236 신윤희, 우은택, 박영두 PF-31 The change of ABA responsiveness through hydrophobic environments in the VxG L motif of PP2Cs in Oryza sativa 236 Seungsu Han, Ji-Young Lee, Yeongmok Lee, Jiseok Beak, Jaeeun Jung, Tae-Houn Kim, and Sangho Lee PF-32 GM 237 문성윤, 강세라, 김영군, 은창욱, 손영진, 오성덕, 박수윤, 장안철, 서상재 PF-33 GM 237 오성덕, 장안철, 서상재, 윤도원, 박수윤 PF 오성덕, 박정호, 김재광, 장안철, 손수인, 윤도원, 박수윤 PF-35 The potential targets for improving plant tolerance to drought stress using drought-responsive micrornas 238 Joohee Choi, Pil Joong Chung, Jooyeon Bae, Jung Suk Kim, Jae Suk Jan, Ju-Kon Kim PF-36 Resistance of transgenic rice events (rbcs:cry1ac) against three lepidopteran rice pests 239 Youn Shic Kim, So-Yoon Seong, Jong Hoon Kim, Sue Yeon Lee, Woo Jin Kim, Jae Young Choi, Seung Tae Kim, Seok Hee Lee, Ying Fang, Dong Hwan Park, Min Gu Park, Ra Mi Woo, Bo Ram Lee, Ju-Kon Kim, Yeon Ho Je PF 정기홍, 문선옥, 김유진, 유요한, Anil Kumar N.C, 김효용, 홍우종, 배도영, 안혜련 l

51 PF 우은택, 장창순, 박영두 PF 이강섭 PF-40 GM 241 朴钟泽 PF-41 PF-42 PF-43 PF-44 Establishing plant viruses as tools to bioengineer corn, soybean, barley and important vegetable crops 241 Wen-Xing Hu, Jung-Kyu Kim, Hye-Kyoung Ju, Hanhong Bae, Leslie L. Domier, Hyoun-Sub Lim Genome information mediating discovery of useful rice genes for enhanced nutrient use efficiencies and the functional identification. 242 Yun-Shil Gho, Sunok Moon, Eun-ha Park, Ki-Hong Jung Strategic approaches to the development of -carotene-enriched transgenic rice as GMO Events 242 Hyung-Keun Ku, Young-Joo Jung, Yeo Jin Lee, Byeong Hoon Kim, Min Kyoung You, Ye Sol Jeong, Jae Kwang Kim, Sun-Hwa Ha Carotenoid metabolic engineering via the polycistronic expression using viral 2A sequences and codon-optimized carotenogenic genes in rice endosperm 243 Ye Sol Jeong, Hyung-Keun Ku, Young-Joo Jung, Jae Kwang Kim, Sun-Hwa Ha PF-45 Epigenetic regulation-mediated drought tolerance in rice 243 So Young Park, Dong-Hoon Jeong PF 김년희, 김송림, 최인찬, 지현소, 이홍석, 이은경, 백정호, 양종목, 안은숙, 김경환 PF Minjin Kim, Byeongrim Go PF-48 Stable expression of brazzein protein with alternative sweetener in rice cell lines 245 Ye Rim Lee, Ji Yun Ko, So Young Park, Kwon Kyoo Kang, Yu Jin Jung PF-49 Manipulation of plant autophagy Atg8 by pathogen effector PopP2 245 Sung Un Huh PF 김민식, 윤철원, 노현수 li

52 PF-51 Development of image-based traits for model tomato(micro-tome) 246 Hwang-weon Jeong, Jihee Park, Sang Ryeol Park, Inchan Choi, JeongHo BAEK, Young-Joo Seol PF-52 Comparative study of antinutrient compounds in different Korean soybean varieties 247 Sang-Gu Lee, Seon-Woo Oh, Soo-Yun Park, So-Young Lee, Hwi-Young Park, Seong-Kon Lee, Young-Soo Chung, Hyun-Suk Cho PF-53 Production of dammarenediol-ii and protopanaxadiol in transgenic rice overexpressing Panax ginseng dammarenediol-ii synthase gene 247 Jung Yeon Han, Do Won Yun, So Hyeon Back, Yong Eui Choi PF-54 GM 248 선현진, 정옥철, 강홍규, 진일두, 이동희, 정혜린, 이효연, 서석철 PF-55 GM 248 선현진, 정옥철, 강홍규, 진일두, 이동희, 서석철, 이효연 PF-56 GM 249 박수윤, 이경민, 오성덕, 서상재, 오선우, 이상구, 이성곤, 조현석 PF-57 GM 249 Hwi-Young Park, Sang-Gu Lee, Seon-Woo Oh, Soo-Yun Park, Seong-Kon Lee, Hyun-Suk Cho PF-58 Compositional analysis of hot pepper cultivars cultivated in the different locations and the natural variations by the evironmental factors 250 So-Young Lee, Seon-Woo Oh, Hwi-Young Park, Soo-Yun Park, Sang-Gu Lee, Seong-Kon Lee, Hyun-Suk Cho PF-59 GM 250 정하나, 좌지방, 손지희, 선현진, 양대화, 강홍규, 이효연 PF 김진주, 박성임, 윤호성 PF-61 Production of recombinant brazzein proteins, a new type of alterative sweeter in transgenic rice 251 Ye Rim Lee, In Hye Lee, Yeo Jin Jung, So Young Park, Kwon Kyoo Kang, Yu Jin Jung PF 김도훈, 김종민 PF-63 PCR 252 조윤성, 임명호, 신공식 lii

53 PF-64 Development of -linolenic acid-increased soybean by the overexpression of PfFAD3-1 gene 253 Hyun Suk Cho, Hye Jeong Kim, Wan Woo Yeam, Jin Sol Park, Jae Yong Han, Hyun Uk Kim, Young-Soo Chung PF-65 Genome plasticity affects bacterial virulence in Burkholderia glumae. 253 Minhee Kang, Eunhye Goo, Jae Yun Lim, Jinwoo Kim, and Ingyu Hwang PF-66 Establishing plant viruses as tools to bioengineer corn, soybean, barley and important vegetable crops 254 Wen-Xing Hu, Jung-Kyu Kim, Hye-Kyoung Ju, Hanhong Bae, Leslie L. Domier, Hyoun-Sub Lim PF-67 GM GMO 254 이향기, 송민경 PF 이범규 PF-69 High expression of recombinant proteins in Arabidopsis protoplasts by using Gal4/UAS gene expression system and PTGS suppressor 255 Junho Lee, Md Reyazul Islam and Inhwan Hwang PF-70 LegCompara: A bioinformatic module for comparative analysis of legume genomes 256 Jin-Hyun Kim, Joo-Seok Park, Yoram Choi, Min-Gyun Jeong and Hong-Kyu Choi PF-71 LegExpress: A translational bioinformatic platform for transcriptome analysis of the legumes 256 Joo-Seok Park, Jin-Hyun Kim, Yoram Choi, Mingyun Jeong, Hong-Kyu Choi PF-72 IR 257 이운석, 김형석, 김준식 PF-73 Function of iron permeases of Fusarium graminearum in pathogenesis 257 Yong-Sung Park, and Cheol-Won Yun PF-74 PF-75 Characterization of a male sterile mutant line reveals that asymmetric microspore division is controlled by a MYB transcription factor in Arabidopsis 258 Sung Aeong Oh, Thi Hoai Thuong Nguyen, Hyo Jin Park, Mingmin Zhao, David Twell, Sang Ju Lee, Jeong Heo Kim, and Soon Ki Park Identification of a gene critical for the germ cell migration after pollen mitosis I in Arabidopsis 258 Sung-Aeong Oh, Hyo-Jin Park and Soon-Ki Park liii

54 PF-76 Taraxacum kok-saghyz 259 Irisappan Ganesh, 배성우, Sandeep Kumar Tata, Tengfang Ling, 유병태 PF-77 Development of humanized rice cell lines for recombinant protein production in plants 259 Seung-Ho Paek, Ju-Young Choi, Bum-Soo Hahn, and Seong-Ryong Kim PF-78 Development of hybrid-synthetic promoters responding to abiotic stress 260 Ki-Hwan Kim, Won-Chan Kim PF-79 Comparative composition of genetically modified soybeans conferring herbicide tolerance and cosmeceutical protein production 260 Young Jin Park, Jeong Su Yoon, Seung-A Baek, Ju-Seok Seo and Jae Kwang Kim PF-80 CHIP1 mediates chloroplast anchoring via protein-protein interaction with CHUP1 261 Sam-Geun Kong, Jae-Woo Han, Gyeong-Hoon Lee, Koji Okajima, Aino Komatsu, Fumio Takahashi, Takayuki Kohchi, Masamitsu Wada PF-81 PF-82 Molecular breeding of vegetable crops for optimization of photosynthetic ability in light-controlled plant cultivation system 261 Young-Sun Riu, Chang Ho Ahn, Yong Eui Choi, Sam-Geun Kong Overexpression of auxin biosynthetic enzyme YUCCA6 enhances multiple abiotic stress tolerances in arabidopsis 262 Joon-Yung Cha, Masatoshi Nakajima, Dae-Jin Yun, Woe-Yeon Kim PF 임명호, 이정동, 박태성, 조윤성, 신공식 PF-84 Seed starch improvement in legumes: recent advances and applicability 263 Rupesh Tayade, Krishnanand P Kulkarni, Jeong-Dong Lee PF-85 Optimization of plant expression vector for tomato transformation 263 Ju Hui Do, Ah Young Kim, Sang Hoon Ma, Hyun Min Kim, Seo Young Park, Thank Dat Mai, Young Hee Joung PF-86 The development of biotic stress resistance plant using Hot pepper NADPH-cytochrome P450 reductase 2 (CaCPR2) 264 Seo Young Park, Sang Hoon Ma, Ah Young Kim, Hyun Min Kim, Thanh Dat Mai, Ju Hui Do, Chul-Ho Yun, Young Hee Joung PF-87 Functional characterization of SlP gene from tomato through in vivo and in vitro 264 Thanh Dat Mai, Ju Hui Do, Seo Young Park, Sang Hoon Ma, Hyun Min Kim, Ah Young Kim, Young Hee Joung liv

55 OG-01 The comparison of two base-editors for precise nucleotide substitution in plants 267 Beum-Chang Kang, Je Wook Woo, Sang-Tae Kim, Minkyung Choi, Su-ji Bae, Hyeran-Kim, Jin-Soo Kim, Sang-Gyu Kim OG-02 Manipulation of inflorescence architecture for tomato productivity 267 Soon Ju Park, Sebastian Soyk, Daniel Rodrı guez-leal, Zachary Lippman OH 유의수, 최아영 OH-02 A comparative synteny analysis tool for target-gene SNP marker discovery: connecting genomics data to breeding in Solanaceae 271 Sung-Hwan Jo, Junkyoung Choe, Ji-Eun Kim, Bong-Woo Lee, Jeong Hee Lee, Moon Nam OH-03 TGIL: An integrative bioinformatic platform for genomics-assisted breeding 272 Jin-Hyun Kim, Joo-Seok Park and Hong-Kyu Choi OH 김남신 Golden Seed Project OI-01 ag-biotechnology 275 성동렬 OI-02 Surfing the web of plant innate immunity: from recognition to engineering 275 Sang Hee Kim OI-03 Enrichment of Brassica Vegetables genepool for secondary metabolites and disease resistance through wide-hybridization 276 Ujjal Kumar Nath, Song Samnang, Pen Sreyvatey, Jong-In Park, Ill-Sup Nou lv

56 OI-04 Bioinformatics applications in horticultural crops using conventional tools and methodologies for crop improvement 276 Sathishkumar Natarajan, Ill-Sup Nou OI 서상기 OI-06 (Doubled Haploid) 277 류시환, 최재근, 박종열, 남궁민, 박기진, 최준근 산림분야발표 OJ-01 Progress and challenges of tree breeding in South Korea 281 S-H Kwon, J-M Park, Y-G Kim, H-I Kang, D-B Yeom, K-W Kim, S-J Na and K-S Kang OJ-02 Discovery of trait-associated omic-markers in Korean chestnut species 281 Eung-Jun Park lvi

57 Plenary Session

58

59 SYMP-01 Hee-Jong Koh Plant breeding initiated by farmers who selected desirable traits for agricultural purposes. Following the rediscovery of Mendel s principles of heredity in 1900, plant breeding has made tremendous progress in developing diverse methodologies to create genetic variability and to genetically fix the promising lines. Recently, crop breeding has been systematized with state-of-the-art technologies aided by transgenic and genomics approaches. Conventional or traditional breeding refers to the development or improvement of cultivars using conservative tools for manipulating crop genomes within the natural genetic boundaries of the species, which has contributed to the human society by fulfilling our needs on food, feed, medicine, and basic materials for industrial purposes. Green revolution, the spectacular increase in cereal crop yields through new plant varieties during the 1960~`70s, has been regarded as the most influential achievement in the history of plant breeding. With the rapid growth of seed industry for recent five decades, plant variety has been regarded as an item for financial profit. This has promoted the activities related to plant breeding in both private and public sectors. More than 15,000 varieties per year have been registered worldwide. Recent concerns about global warming, abnormal weather patterns, and unfavorable environments have pushed breeders to speed up the breeding process. However, conventional breeding has been facing difficulties to meet all the human needs due to the limited genetic variability and genomics information. Concerted approaches among all the breeding technologies are required. Overall achievements, challenges and perspectives of crop conventional breeding will be discussed. *Corresponding Author: heejkoh@snu.ac.kr SYMP-02 Gregory B. Martin 1,2,3, Robyn Roberts 1, Ning Zhang 1, Samantha Mainiero 1, Adrian F. Powell 1, and Susan R. Strickler Pseudomonas syringae pv. tomato (Pst) causes bacterial speck disease of tomato. The only genetic resistance to Pst is conferred by the resistance genes Pto/Prf, which recognize the Pst effectors AvrPto and AvrPtoB. However, recently Pst strains have emerged that lack these effectors, rendering Pto/Prf-mediated resistance ineffective. In current work we are seeking new sources of genetic resistance against Pst that can be introgressed into tomato breeding lines. Using available whole-genome resequencing data, we identified a set of 216 genetically diverse accessions. Plants of each accession were inoculated by spraying Pst mutant strains that lack different effectors and/or flagellin to help elucidate whether the observed host responses involved effector-triggered or pattern-triggered immunity. The screen also uncovered unusual Pst disease symptoms beyond the typical specks, and subsequent analyses indicate some of these phenotypes are simply inherited. Using an assay that measures the production of reactive oxygen species, we discovered accessions that have increased, or conversely, no response to the flagellin peptides flg22 or flgii-28, which are recognized by the pattern recognition receptors FLS2 or FLS3, respectively. In a related project, we are using CRISPR/Cas9 to create mutations in over 100 immunity-associated genes in tomato and assessing the effect on the host response to Pst. Together, these projects open up new avenues for investigating the molecular mechanisms underlying the plant immune system and augment plant breeding efforts to develop disease-resistant tomato varieties. 3

60 SYMP-03 박희영 인류는 2030 년대인구 80 억시대를눈앞에두고안정적인식량공급과지속가능한농업이라는두가지화두를해결하고자노력하고있다. 식물육종은 1996 년 GM 콩의상업화이후생산량증가와생산성향상을통해새로운전기를마련하였다. 지난 20 년간 GM 작물의연구개발상업화는비약적인발전을거듭하여전체종자공급량의 50% 이상을 GM 종자들이담당하고있다. 빅 6 종자회사들은이러한성공을바탕으로새로운 GM 작물도입과생산성향상을이루었고, 현재진행중인인수합병이완료되면글로벌종자업계에기대와우려가섞인새로운바람이불게될것이다. GM 작물은종자시장점유율이높고저장성및환금성이높은작물들인옥수수, 콩, 면화, 카놀라등대해 Input trait ( 제초제저항성및해충저항성형질 ) 위주로개발되어져왔으며, 새로운제초제저항성형질도입및 Output trait ( 영양성개선형질, 내재해성형질 ) 들이도입되고있다. GM 작물육종기술의성공을바탕으로유전체정보의확보, 작물에따른형질전환기술및재분화기술을확보하게되었고작물자체유전자의원하는부위를바꿀수있는유전자가위법 (gene editing) 이실용화되고있어향후식물육종분야에서이러한기술들의상호보완적협력으로새로운형질의작물들을시장에선보이게될것으로본다. 그러나유전자가위법 (gene editing) 의실용화에도규제라는큰장벽이기다리고있다. 미국뿐만아니라 EU 등에서도유전자가도입되지않은경우 GM 으로규제하지않는방향으로규제개선을시도하고있어막대한등록비용을줄일수있는길을열어가고있다. 향후, 전통육종, 분자육종, GM, gene editing 등모든육종기술이각자의역할을담당하여인류의식량문제를해결해나가는통합적 solution 을제공해줄수있을것으로기대한다. * 주저자 : Tel , heeyoung.park@syngenta.com SYMP-04 Yong Q. Gu 1, Naxin Huo 1,2, Susan B. Altenbach 1, Lingli Dong 3, Daoweng Wang 3, Mingcheng Luo 2, Jan Dvorak 2, Jong-Yeol Lee 4, and Toni Mohr Wheat is one of the food crops most consumed by humans worldwide. However, the molecular basis of wheat flour end-use quality is still only partially understood. A single wheat cultivar contains about 70 to 100 similar but distinct gluten proteins (prolamins) that determine its end-use quality. Wheat flour proteins also trigger human health problems, including food allergies (FA), celiac disease (CD) and non-celiac wheat sensitivities (NCWS). Previous studies indicated that the wheat prolamins are encoded by complex multiple gene families that are mapped to three major genomic regions. To better understand the evolution, expression, and function of prolamins in relation to end-use quality and immunogenic potential, genomic regions harboring the prolamin gene loci were sequenced, annotated, and compared with the orthologous regions from different species (rice, Brachypodium, and sorghum) and from different homeologous wheat genomes (A, B, and D). Our results indicated that rapid evolutionary dynamics are present only in the wheat genomes. The high frequency of sequence rearrangements including deletion, duplication and translocation events have resulted in considerable synteny erosion in the prolamin genomic regions. We propose that the HMW-glutenin genes originate from a tandem duplication of an ancestral globulin gene, while other prolamin genes (LMW-glutenin, -, -, and -gliadins) are likely derived from gene translocations followed by multiple rounds of gene duplications. A complete set of wheat prolamin genes including both intact genes and pseudogenes were identified from a single wheat cultivar cv Chinese Spring, allowing for more accurate and robust characterization of individual prolamin gene expression using transcriptomics analyses. We found that the A genome contributes the least to prolamin expression in cv Chinese Spring because of its smaller number of expressed intact genes and their low expression levels, while the B and D genome contribute similarly. Our study also provided insights into the evolution of CD epitopes and identified that a single indel event in the hexaploid wheat D genome likely resulted in the generation of the highly toxic 33-mer CD epitope. A DNA sequence capture array was designed for studying the genetic diversity of prolamin genes in other wheat cultivars, with an aim to facilitate breeding wheat varieties with improved end-use traits and reduced immunogenic potential. 4

61 SYMP-05 Illsup Nou * With the advent of genome sequencing technologies, the scientific era has crossed the borders of utilizing model plant in genomic research. The basic research in the Department of Horticulture in Sunchon National University mainly emphasizes in the program (The Golden Seed Project) to develop horticultural crops in the families Brassicaceae (Cabbage, Chinese cabbage), Cucurbitaceae (Melon, Watermelon,), Solanaceae (Tomato, Hot pepper) and Strawberry with high quality traits. The research program mainly focused to identify the genes essential for economically important traits in horticultural crops through physiology and phenomics-guided omics (genomics, transcriptomics, proteomics and metabolomics) investigations. The next-generation sequencing approaches viz genome sequencing, transcriptome sequencing, whole genome resequencing, deep sequencing, restriction site associated DNA sequencing (RAD-seq) and genotype-by-sequencing (GBS) are in practice under this program for the development of the trait-specific molecular markers and to address appropriate solution in population genomics. Those molecular markers including small sequence repeats (SSR), single nucleotide polymorphisms (SNPs), sequence characterized amplified regions (SCAR), small insertions and deletions (INDELs) and cleaved amplified polymorphic sequences (CAPS) associated with particular quality traits have been developed. Such developed molecular markers were capable to alter the fate of the plant through molecular breeding strategies like marker-assisted selection (MAS), marker-assisted backcrossing (MABC), marker-assisted recurrent selection (MARS), and genome-wide selection (GWS) or genomic selection (GS). For instance, shortening the breeding cycle and the increasing the breeding efficiency of the breeders. We have also developed SNP and SSR chips to test the purity in these horticultural crops. In addition, web-database and web-server based research information system have been developed with user-friendly interface. The efforts in the development of molecular markers have enabled us to render quality service to companies or breeders, commercially and privately. Therefore, this project would be a great asset for the global research community and the industrialists for the development of novel horticultural crops with high quality traits through peer-reviewed publications and communication. *Corresponding Author: Tel , nis@sunchon.ac.kr SYMP-06 Dong-Yup Lee 1,2* 1 2 Big Data have been rapidly generated in the modern biological science and biotechnology fields, including plant science and agrobiotechnology, due to the recent advances in high-throughput omics technology. Now, one of major challenges is to analyze and integrate these large and heterogeneous datasets in order to enhance the crop productivity and quality which are severely hampered by various biotic and abiotic stresses as well as inherent genetic limitation. Thus, it is required to develop and understand various systems and data mining approaches for systematic applications. To this end, we have established the integrative framework for characterizing model plant such as rice by resorting to systems biology techniques which include in silico modeling and machine learning. The information derived from the current in silico analysis of virtual plant cells in conjunction with multi-omics profiling and synthetic biology tools can potentially guide for developing new breeding and/or engineering targets for next generation crop development. In this talk, modeling of various plant systems will be introduced, and current challenges and future direction in the field, e.g., smart farming, will be discussed. [This work was supported by Next-Generation BioGreen 21 Program (SSAC, No. PJ ), the Rural Development Administration, Republic of Korea] *Corresponding Author: Tel , lee_dong_yup@bti.a-star.edu.sg 5

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63 공동심포지엄 분과발표 및 구두발표

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65 OA-01 ZhongZe Piao * Rice production in China has more than tripled in the past five decades mainly due to increased grain yield rather than increased planting area. This increase has come from the development of high-yielding varieties and improved crop management practices such as nitrogen fertilization and irrigation. However, yield stagnation of rice has been observed in the past ten years in China. As its population rises, China will need to produce about 20% more rice by 2030 in order to meet its domestic needs if rice consumption per capita stays at the current level. By using a large amount of data, China s rice production, market supply and demand, and trade changes is analysed in recent years. At the same time, many constraints that China s current rice production is facing on is pointed out. What s more, based on the new situation of rice production and consumption in China, the development direction of rice genetics and breeding in China is proposed. *Corresponding Author: Tel , zzpiao@hotmail.com OA-02 Sungwoo Lee 1,2*, Kyujung Van 3, Mikyung Sung 1, Leah K. McHale 3,4, Randall Nelson 5, Jonathan LaMantia 6, M. A. Rouf Mian 1, Soybean [Glycine max (L.) Merr.] protein and oil are widely used in feed, food, and industrial raw materials. Soybean seed protein is negatively correlated with seed oil and yield. A genome-wide association study (GWAS) was conducted using phenotypic data collected from five environments for 621 soybean accessions in maturity groups I.IV and 34,014 single nucleotide polymorphism (SNP) markers to identify QTL for protein and oil. Compressed Mixed Linear Model (CMLM) identified three and five quantitative trait loci (QTL) significantly associated with seed protein and oil contents, respectively. Of them, QTL on chromosomes (Chrs) 15 and 20 were the two most significant QTL for protein and oil contents, which exhibited a strong negative correlation between the two traits. Mmulti-trait mixed model (MTMM), also allowed identification of common effect QTL on Chr 5 that increased oil with no effect on protein, and on Chr 10 that increased protein with little effect on oil. Haplotype analysis revealed that the positive-effect haplotypes for the 4 loci varied in frequency across geographic regions where the soybean accessions originated. This finding informs which alleles will potentially better contribute to marketor growing region-specific improvement of soybean. *First author: Tel , sungwoolee@cnu.ac.kr 9

66 OA-03 Yoonha Kim * Regardless of silicon (Si) is the second most abundant element in soil, various plant species shows significantly different Si uptake ratio. Therefore, many scientists believed that Si could induce physiological responses in restricted plant species such as rice, barley, and wheat. In 2006 and 2007, Japanese research team reported Si transport genes (low silicon gene; Lsi1 and Lsi2) in rice plant. These two genes are antithetically located in the exodermis and endodermis of rice root thus, Si regulates influx and efflux by Lsi1 and Lsi2 respectively. Since identification of Si transport gene, we can figure it out how Si can transfer from soil to plant. Moreover, recently developed Si-mutant plant can facilitate evaluation of Si effect on endogenous phytohormones regulation, scavenging of reactive oxygen species (ROS) and reactive nitrogen species (RNS). In this presentation, I focused on how Si can mitigate various abiotic stress conditions such as mechanical wounding, salinity and heavy metal stresses. When we applied Si to rice plant under normal condition, increased bioactive GA 1 and suppressed Ca uptake in comparison with control was measured. Moreover, Si application was found to mitigate various abiotic stresses such as mechanical wounding, salinity, and heavy metal stress by different physiological strategies. In the mechanical wounding stress, Si supplementation reinforce the cell membrane thus, down-regulated jasmonic acid (JA) was detected in rice plant to enhance the resistance against mechanical wounding stress. While during salinity stress, Si treatment to rice plant increased endogenous abscisic acid (ABA) content meanwhile, decreased transpiration and reduced Na uptake from root. Whereas, when rice plants were grown under heavy metal (high concentration of Cu and Cd) stress condition, Si application down-regulated heavy metal transport genes such as OsHMA2 and OsHMA3 (P1-type heavy metal ATPase) thus, decreased heavy metal contents were detected in rice plants (shoot and root). Therefore, Si treated rice plants showed resistance against heavy metal stress through decreased heavy metals uptake. Furthermore, exogenous application of Si has been found to induce stress tolerance by scavenging of reactive oxygen species (ROS) such as ascorbate peroxidase (APX), catalase (CAT) under overall stress conditions. Because, under natural condition, plants continuously produce several ROS during photosynthesis and respiration processes in cell organelles such as mitochondria, chloroplast, and peroxisomes. Particularly, the ROS production significantly increases under stress condition. Therefore, ROS scavenging is one of the most important strategy to mitigate stress condition. Acknowledgement: This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2017R1D1A3B ) *Corresponding Author: Tel , kyh1229@knu.ac.kr 10

67 OA-04 정종민 1*, 김석만 2, 정지웅 1, 김보경 1, 이점호 직파재배는육묘와이앙과정이없어, 이앙재배보다노동력및생산비가적게드는장점이있으나초기입모불량, 잡초발생및도복에따른재배안정성문제로인해재배면적확대에어려움이있다. 담수직파재배법은담수상태에직접볍씨를파종하므로다른직파재배법에비해담수로인해초기잡초발생이억제되어잡초방제효과가우수한장점이있으나담수로인한혐기상태로인해종자의발아및입모가불량해지는단점이있다. 현재개발된직파품종은저온발아성및도복관련형질은우수한편이나혐기발아담수중발아율은다소부족한편이다. 따라서담수중입모율이우수한직파전용품종개발을위해서는혐기발아성이우수한유전자원탐색및관련유전자분석및도입이필요하다. 본연구는담수중혐기발아성이개선된담수직파용품종개발을위하여혐기발아연관유전자탐색및혐기발아연관유전자를국내자포니카형벼에도입하는것을목적으로수행되었다. 국내벼품종의담수중혐기발아성향상을위하여혐기발아 (anaerobic germination, AG) 성이우수한미얀마 landrace Khao HlanOn (KHO) 과국내벼품종 ( 동안, 수안, 보람찬 ) 과교잡을통하여혐기발아내성유전자 AG1 과 AG2 가이전된근동질계통 (BC 2 F 5 와 BC 3 F 5 ) 을육성하였다. AG1 과 AG2 연관 marker 를이용하여 MAB 를수행하고초형선발및유전자형분석을거쳐 3 조합에서혐기발아내성이반복친보다향상되고농업형질이양호한 37 계통을선발하였다. 한편새로운 AG 유전자탐색을위하여혐기발아내성이우수한광발성잡초벼유전자원 PBR 와국내고품질품종 남평 이교배된 Mapping 집단을육성하였다. Illumina 7K SNP chip 을이용한 mapping 결과총 942 개의 SNP 마커가 Mbp 의벼염색체상에 anchor 되었다. 온실조건과포장조건을이용한 AG 내성에관한 QTL 분석결과염색체 1 번, 3 번그리고 11 번에서 AG 관련형질을표현형대비 6.7% 14.5% 로설명하는 3 개의 QTLs (qag1, qag3, qag11) 이각각탐색되었다. 향후이들결과를활용하여담수직파초기입모관련유전자집적을통해직파적성개선을위한직파적응우량계통 / 품종육성에활용할계획이다. * 주저자 : Tel , jjm0820@korea.kr OA-05 Hyun-Su Park *, Man-Kee Baek, Choon-Song Kim, Jeong-Kwon Nam, Gun-Mi Lee, Seul-Gi Park, Chang-Min Lee, Young-Chan Cho Rice panicle and grain-related traits have important roles in rice yield and quality. Korean japonica rice cultivars showed narrow genetic background on panicle and grain. To overcome the limit and pioneer new uses, we developed japonica rice with diverse panicle and grain shape, New Panicle Architecture (NPA) and Diverse Grain Size and Shape (DGS), and characterized those agronomic traits. For enhancing yield potential by increasing number of spikelets per panicle (NS), we developed dense panicle rice with clustered spikelets. Dense panicle rice with clustered spikelets have many spikelets clustered two to five together on more increased panicle branches. Rice with NPA was derived from a cross between Binhae Col.#1, lodging resistance rice germplasm with dense panicle, and ARC10319, tall rice germplasm with clustered spikelets. It showed dramatically increased spikelets (average NS ) compared to Nampyeong (93), Boramchan (96), Hanareum2 (114), Binhae Col.#1 (144), and ARC10319 (161). The other rice with NPA derived from same combination is very tall and high biomass rice with clustered spikelets, Jeonju626. It is the tallest among Korean rice cultivars with lodging resistance and fast-elongation. The plant height of Jeonju626 was 183 cm (culm length 155 cm, panicle length 28 cm), while those of Korean rice cultivars were cm (n=390). Jeonju626 is expected to contribute the transition from semi-dwarf to tall plant architecture in Korean rice cultivars. To diversify the grain size and shape of japonica rice, we developed 91 Breeding Materials with DGS (BM_DGS, DGS6-96) derived from four cross combinations between donor parents, extremely large grain germplasm Jizi 1560 (1,000 grain weight 48.1 g) and Jizi1581 (38.0 g), and Korean high-yielding rice cultivars, Deuraechan(24.7 g) and Boramchan (22.9 g), by anther culture. The grain size and shape of BM_DGS exhibited beyond the characteristics of previously developed Korean rice cultivars; most of Korean japonica rice cultivars have medium-short and semi-round shaped grain. BM_DGS are being practically utilized in the breeding programs to diversify the grain size and shape. Using DGS79, the breeding material with extra-long and spindle-shaped grain, we developed elite japonica rice line with long and spindle-shaped grain, Jeonju625. It showed good characteristics for rice yield and grain quality. Jeonju625 is expected to be used as cultivar aimed at exporting rice and the breeding programs for enhancing the resistance to biotic stress and diversifying the heading date of Jeonju625 are in progress. Developed rice with NPA and DGS could be contribute to diversify the genetic background and pioneer new uses of Korean japonica rice. *Corresponding Author: Tel , mayoe@korea.kr 11

68 OA-06 Le Duc Thao 1* 1 In Vietnam, soybean is one of the oldest and important food crops that provide human food and animal feed. However, in recent years, soybean production in Vietnam tends to decrease, while soybean demand is increasing. From 2010 to 2016, the area decreased from thousand hectares to 94.0 thousand hectares, output decreased from thousand tons to thousand tons. By 2016, the yield is 1.57 tons/ha, equal to 56.5% of the world average (2.78 tons/ha). Currently, soybean production only meets about 8% of domestic demand, while demand for soybean oil industry and animal feed is on the rise. In 2011, Vietnam imported 0.90 million tons of soybeans and by 2016 it increased to 1.56 million tons, mainly from the United States, Argentina and Brazil. In addition, Vietnam has to import millions of tons of soybean products. There have been more than 50 varieties released and applied in production through main methods are imported selection, hybridization and mutation. Soybean varieties commonly used in production now include: local varieties (Ha Giang Green, Muong Khuong Vang, Bac Ha Green...), imported varieties (ĐT12, AK03, VX92, DT 2000, DH4,...), hybrids (DT80, Đ92, Đ93, Đ96-02, DT42, TL57, 98-04, DT26, Đ2102, Đ2501, DT96, DT2001, ÐVN5,...) and mutant varieties (DT84, DT90, DT99, DT95, DT83...). Due to the features diverse ecologicals, soybean varieties were classified into groups: - The group of varieties suitable for cold crop: V74, AK02, AK03, AK04, AK05, VX92, VX93, ĐT2000, ĐN42, ĐT92, DT90, TLA57, 98-04, ĐT26, Đ The group of varieties suitable for hot crop (summer in Northern Delta): ĐH4 (ĐT76), M103, ĐT80 and local varieties. - The group of varieties suitable growing 3 crops/year: DT84, DT94, DT95, DT96, DT99, DT2001, ĐVN6, ĐVN10, AK06, ĐT93 The orientation of soybean breeding is focused on improving productivity, pest and disease tolerance and adaptation to different regions and seasons. Solutions of developing soybean production in Vietnam are: 1) Improving the yield; 2) Increasing the area by taking advantage of the land cultivating other crops and intercrops; 3) Using new varieties with high tolerance to diseases and unfavorable conditions (drought, flood, cold ) and good quality; 4) Extention agriculture and transfering advanced techniques; 5) Policies of supporting seeds, capital, supplies, market solutions. *Corresponding Author: Tel. (+84) , leducthao@agi.vaas.vn OB-01 Phu-Tri Tran 1,2, Kristin Widyasari 1, Jang-Kyun Seo 3, Kook-Hyung Kim 1,2,4* Soybean mosaic virus (SMV), a member of the genus Potyvirus, significantly reduces soybean production worldwide. Rsv3, which confers strain-specific resistance to SMV, was previously mapped between the markers A519F/R and M3Satt in chromosome 14 of the soybean [Glycine max (L.) Merr.] genotype L29. Analysis of the soybean genome database revealed that five different NBS-LRR sequences exist between the flanking markers. Among these candidate Rsv3 genes, the full-length cdna of the Glyma.14g was successfully cloned from L29. Over-expression of Glyma.14g in leaves inoculated with SMV inhibited viral infection in a soybean genotype lacking Rsv3. In addition, the transient silencing of the candidate gene caused a high accumulation of an avirulent strain in L29 carrying Rsv3. Our results therefore provide additional line of evidence to support that Glyma.14g is likely Rsv3 gene that confers strain-specific resistance to SMV. *Corresponding Author: Tel , kookkim@snu.ac.kr 12

69 OB-02 Boyoung Kim 1, Je Min Lee 2, In Sun Hwang 1, Hyung Jin Lee 1, Eunyoung Seo 3, Doil Choi 3, Chang-Sik Oh 1* Bacterial wilt caused by Ralstonia pseudosolanacearum (previously known as R. solanacearum) is a very devastating disease in tomato. Previous studies have identified two major quantitative trait loci (QTLs), Bwr-6 at the chromosome 6 and Bwr-12 at the chromosome 12, responsible for disease resistance against bacterial wilt in tomato cultivar Hawaii7996. However, the genetic identities of two QTLs have not been uncovered yet. In this study, using whole-genome resequencing, we analyzed genome-wide single-nucleotide polymorphisms (SNPs) that can distinguish a resistant group, including seven tomato varieties resistant to bacterial wilt, from a susceptible group, including two susceptible to the same disease. In total, 5,259 non-synonymous polymorphic SNPs (about 0.13% of the total homozygous SNPs) were found between the two groups. Among them, only 265 SNPs are located in the coding DNA sequences, and 53 and 168 out of these SNPs were located on chromosomes 6 and 12, respectively. The genes that both carry SNP(s) and are near Bwr-6 and Bwr-12 were selected for development of SNP markers. First, 13 SNPs in four genes encoding putative leucine-rich repeat (LRR) receptor-like proteins out of 26 genes near Bwr-12 in chromosome 12 were analyzed. Each SNP marker was validated by a high resolution melting method with 42 tomato cultivars and one segregation population. Consequently, one SNP marker, including a functional SNP in a gene, Solyc12g , could efficiently distinguish tomato varieties resistant to bacterial wilt from susceptible varieties. These results indicate that Solyc12g , the gene encoding a putative LRR receptor-like protein, might be tightly linked to Bwr-12. Next, analysis of SNPs in 15 genes near Bwr-6 is in progress. Overall, the SNP markers developed in this study will be useful for selection of tomato cultivars resistant to bacterial wilt. *Corresponding Author: Tel , co35@khu.ac.kr OB-03 Muhammad Irfan Siddique 1, Hea-Young Lee 1, Koeun Han 1, Jelli Venkatesh 1, Jin-Kyung Kwon 1, Byoung-Cheorl Kang 1* 1 Phytophthora capsici is a globally prevalent devastating Oomycetes pathogen causing root rot in pepper. Capsicum annum CM334 is widely used as a source of resistance. We constructed a high-resolution linkage map using 188 recombinant inbred lines (RILs) derived from a cross between resistant CM334 and susceptible ECW30R. A total of 8,979 high quality genotyping-by-sequencing derived single nucleotide polymorphism (SNP) markers were used to map resistance against low, medium and high virulent isolates ofp. capsici. Quantitative trait loci (QTL) analysis for P. capsici root rot (PcRR) mapped two major effect QTLs, qtl5.1, and qtl5.2 on chromosome P5 conferring broad-spectrum resistance to P. capsici isolates. In addition, QTLs with minor effects and isolate specificity were detected on P2, P4, P8, and P11. QTL analysis was complemented with a genome-wide association study (GWAS) of root rot resistance in a pepper core collection consisting of 352 diverse accessions. A total of 168,714 SNPs derived from two GBS libraries were used for GWAS. GWAS detected 98 significant SNPs associated with resistant to highly virulent isolate, and the regions on P2, P5, and P11 were co-located with QTLs identified in the present study. By leveraging combined use of QTL mapping and GWAS, clusters of nucleotide-binding site leucine-rich repeat (NBS-LRR) and receptor like kinases (RLKs), and Mildew resistance locus O(MLO) like protein candidate genes involved in plant disease resistance mechanisms were predicted within the QTL regions. Highly significant SNP markers identified through QTL mapping and GWAS for PcRR resistance herein could accelerate the marker-assisted breeding for durable resistance in pepper by combining alleles of race specific and non-race specific resistance in pepper. 13

70 OB-04 Marjohn Nino 1, Franz M. Nogoy 1, Me-Sun Kim 1, Ju-Young Yang 1, Le Van Trang 1, Kwon-Kyoo Kang 2, Yong-Gu Cho 1* 1 2 Pathogen infection modulates defense reactions in the host cells. Numerous findings proved that papain-like cysteine proteases (PLCPs) function as a central hub in plant defense. While diverse roles of PLCPs in different pathosystems have become more evident, information on which gene networks and signaling pathways are activated to orchestrate downstream responses remains limited. To understand the biological significance of cysteine protease against Xanthomonas oryzae pv. oryzae, RNAi-mediated knockout and overexpression of xylem cysteine protease gene were constructed in rice. Pathogenicity test showed that transgenic rice attenuated the virulence of X. oryzae pv. oryzae race K3a which could be attributed to a high accumulation of hydrogen peroxide and free salicylic acid. To provide useful insights into genome-wide transcriptome profile during early interaction with the pathogen, next-generation sequencing of RNA from transgenic and wild type plants infected for 30 minutes was carried out. A total of 2,086 combined differentially expressed genes were identified, 471 of which were exclusively regulated in the transgenic library. The resistance observed in transgenic rice is ascribed to an extensive participation of genes with predicted functions in intracellular signal transduction, transcription activity, secondary metabolic process including phenylpropanoid and lignin biosynthesis, and defense response. Moreover, protein-protein interaction network revealed the indispensable network of defense layer in transgenic rice. Acknowledgement: This work was supported by a grant from the Next-Generation BioGreen 21 Program (PJ ), RDA, Republic of Korea. *Corresponding Author: Tel , ygcho@cbnu.ac.kr OC-01 Byung-Wook Yun Reactive oxygen and nitrogen intermediates (ROIs and RNIs) are central features of the plant immune responses and abiotic stress tolerance. The latter is one of the major challenges that restricts plant growth and yield thereby affecting human race directly. Understanding the molecular mechanism of how these adversities effects plant life and how green producers cope with these insults, is of paramount importance. One of the relatively less-known feature of abiotic stress is the production of nitric oxide (NO), an important redox based signaling molecule. Among various others, chief of the mechanisms through which NO transfer its bioactivity is S-nitrosylation, the covalent attachment of a nitric oxide (NO) moiety to a protein cysteine thiol to form an S-nitrosothiol (SNO). This rapidly emerging prototypic, redox-based post-translational modification may act as a key switch in regulating biotic and abiotic stress responses. For instance, S-nitrosylation of NADPH oxidase regulates hypersensitive response during plant immunity. Similarly, an Arabidopsis homologous AtRBOHD at Cys 890, abolishing its ability to synthesize ROIs. Accordingly, mutation of Cys 890 compromised S-nitrosothiol-mediated control of AtRBOHD activity, perturbing the magnitude of cell death development. On the other side NO is required for ABA-induced stomatal closure, thereby enhancing plant resistance towards drought stress. NADPH oxidase is thought to have a role in the process hence playing vital role in both type of stresses. Recently, we found that mutation in one of the ABA biosynthetic pathway gene AAO3 resulted in reduced level of ABA and were unable to close stomata upon induction of drought stress. In addition, our current study to isolate novel nitrosative stress-responsive immune and abiotic stress regulators such as WRKY and MYB transcription factors by transcriptomic approach in both Arabidopsis and rice will be introduced, exemplifying with an agriculturally important trait of seed shattering involved in NO signaling and plant immunity. *Corresponding Author: Tel , bwyun@knu.ac.kr 14

71 OC-02 Joong Hyoun Chin 1*, Jae-Hyuk Han 1, Na-Hyun Shin 1, Soo-Cheul Yoo 2, Jae-Hoon Moon Pup1 (Phosphorus uptake 1) is a major QTL for phosphorus-deficiency tolerance in rice. PSTOL1, the major gene of Pup1 was firstly identified from a Bangladesh landrace, Kasalath. However, Pup1 is present in almost all the upland and/or drought tolerant varieties. Due to its contribution to early root vigor and water-p interaction mechanism, Pup1 might contribute to the drought tolerance in early rice growing stage. Vigorous shoot growth of the Pup1 introgression lines was observed in different varietal background in upland condition. In reality, the genetic interactions among multiple abiotic stresses should be considered. As one example, by combination of several abiotic-stress tolerant QTL near isogenic lines, two and three QTL pyramiding lines including Pup1, Sub1 for submergence, and AG1 for anaerobic germination were developed. Primary result showed that the pyramiding approach of Pup1 and Sub1 is not successful under low phosphate rainfed condition, implying the presence of genetic interactions between the QTLs. Here in this presentation, a progress of the multiple trait pyramiding for multi-abiotic stress tolerant breeding line development will be introduced and its implication for rice breeding will be discussed. *Corresponding Author: Tel , jhchin@sejong.ac.kr OC-03 Kiyoon Kang, Nam-Chon Paek * Plant-specific transcription factors (TFs) play essential roles in multiple aspects of development, leaf senescence, abiotic and biotic stresses. The rice genome has approximate 2487 genes encoding TF proteins which are classified into 84 families based on their domain composition. Among them, the OsNAC, OsMYB and OsWRKY TFs are largest families consisting of 144, 202, 103 genes, respectively, and more than half the member of their gene exhibits altered gene expression under salt and drought stress conditions. However, the protein functions of rice TFs in abiotic stress and leaf senescence have been revealed in only few reports. To find the rice OsNAC, OsMYB and OsWRKY TFs participating in regulatory mechanisms of abiotic stress and leaf senescence, we constructed an neighbor-joining trees with aligned domain of rice TFs and Arabidopsis TFs of which protein functions are known in abiotic stress and leaf senescence. We found that 60 rice TFs have highly amino acid sequence similarity with Arabidopsis TFs and show altered gene expression under abiotic stress and senescence conditions. To identify the functions of selected TFs in abiotic stress and leaf senescence, we obtained the T-DNA insertion mutants from Kyung Hee University and screened the mutant phenotypes. The results indicated that mutation of three genes encoding OsNAC, OsMYB and OsWRKY proteins, respectively exhibited hypersensitivity to salt stress and/or delayed leaf senescence. In this talk, I wil discuss the novel regulatory mechanisms mediated by the rice TFs involved in abiotic stress response and leaf senescence. *Corresponding Author: Tel , ncpaek@snu.ac.kr 15

72 OC-04 Yo-Han Yoo 1, Anil Kumar N.C 1, Jong-Chan Park 1, Yun-Shil Gho 1, Sang-Won Lee 1, Gynheung An 1 and Ki-Hong Jung 1* 1 Water deficiencies are one of the most serious challenges to crop productivity. To improve our understanding of soil moisture stress, we performed RNA-Seq analysis using roots from four-week-old rice seedlings grown in soil that had been subjected to drought conditions for 2 to 3 d. In all, 1098 genes were up-regulated in response to soil moisture stress for 3 d, which causes severe damage in root development after recovery, unlikely that of 2 d. We then validated the expression patterns of two candidate genes using a promoter-gus reporter system in planta and monitored the stress response with novel molecular markers. An integrating omics tool, MapMan analysis, indicated that transcription factor, kinase and RING box E3 ligases are significantly stimulated by induced drought. We also analyzed the functions of 66 candidate genes that have been functionally investigated previously. Of these, we used a T-DNA insertional mutant of rice phytochrome B (OsPhyB) that negatively regulates a plant s degree of tolerance to water deficiencies through the control of total leaf area and stomatal density based on previous finding. Unlike previous result, we found that OsPhyB represses the activity of ascorbate peroxidase and catalase mediating ROS processing machinery required for drought tolerance of roots in soil condition, suggesting the potential significance of remaining uncharacterized candidate genes for manipulating drought tolerance in rice. *Corresponding Author: Tel , khjung2010@khu.ac.kr OC-05 Song Lim Kim, Inchan Choi, Hyenso Ji, Hongseock Lee, Nyunhee Kim, Eungyeong Lee, JeongHo Baek, and Kyung-Hwan Kim * Plant phenomics is a nondestructive analyzing methodology using image information of various phenotypes. In this study, rice was used as a model plant for application of phenomics approaches. Agronomic traits of rice were measured using parameters such as leaf area (LA), leaf width (LW), leaf color, projected plant height (PPH), convex hull (CVH), center of mass Y (COMY), compactness (COMP), and eccentricity (ECC) with a recombinant inbred lines (RILs) population derived from a cross between Milyang23 and Gihobyeo. In seedling stage (2 and 4 weeks after sowing), two major growth related QTLs were discovered at semidwarf-1 (sd-1) region of chromosome 1 and loci of chromosome 12. In vegetative stage (6 and 8 weeks after sowing), growth related QTLs were detected at chromosomes 1, 2, 3, 7, 9, 11, and 12. Phytochrome B mutants (osphyb) was also investigated to analyze agronomic traits for drought stress. In recovery stage after drought stress, osphyb was more increased than WT in the leaf area (LA) and leaf width (LW) of RGB and water contents of Near infrared (NIR). It definitely well reflected drought resistance of osphyb. Besides, we are also trying to minutely detect nitrogen deficient traits through color classification of green color in leaf. We are gradually optimizing various traits analysis, and it will be widely applied for improving accuracy for crop breeding and phenotyping. *Corresponding Author: Tel , biopiakim@korea.kr 16

73 OC-06 Yun-A Jeon 1, Gynheung An 2, Lae-Hyeon Cho 2, Hyun-Sook Lee 1, Sun Ha Kim 1, Won-Yong Song 3, Sang-Nag Ahn 1* Flowering time (heading date) is an important agronomic trait that determines yield in rice (Oryza sativa L.). In this study, we characterized probable L-ascorbate peroxidase (APX4-P) gene in near isogenic line (NIL) derived from a interspecific cross between Hwaseong and Oryza rufipogon. The NIL plants showed delayed flowering about 6 days compared to Hwaseong under the natural long-day condition. To study how APX4-P is involved in the mechanism of flowering, we examined expression levels for flowering time regulators under short-day (12h light/12h dark) and long-day conditions (14h light/10h dark). No difference in days to heading was observed between Hwaseong and NIL in short-day condition whereas NIL plant showed delayed flowering in long-day condition. Also, to investigate the antioxidant activity in Hwaseong and NIL, the 3,3-diaminobenzidin (DAB) staining and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay were both conducted. The DAB staining was performed to understand whether APX4-P gene plays role in scavenging H 2 O 2 in rice. Based on DAB staining, dark-brown color was barely detected in NIL than Hwaseong. The DPPH scavenging ability of NIL plants showed higher value than Hwaseong. Hence, these results suggested that flowering tume and antioxidant activity might be controlled by APX4-P gene. Acknowledgement: This work was carried out with the support of "Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ )" Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , ahnsn@cnu.ac.kr OD-01 조준현 1*, 송유천 1, 한상익 2, 고종민 최근의급격한고령화가속화로건강기능식품에대한관심증가및연이은풍작이지속되고있는반면소비가크게줄면서쌀소비를촉진시키기위한방안으로고기능성식품개발을통한부가가치향상대책의필요성이크게대두되었다 년이후기능성쌀소비시장변화와다양한쌀가공품의시장진출에도불구하고쌀가공산업은아직시작단계이다. 이러한배경에는흑미중심의제한된기능성쌀의소비자인식부족과함께, 특히밀가루등타전분에비해낮은가공특성및고미나수입쌀사용에의한쌀가공품의신뢰성부족으로평가될수있다. 국내의경우기능성원료곡시장은흑미등일부유색미품종들을제외하면아직초기단계이다. 따라서기능성을이용한가공시장으로도약 발전하기위해서는다양한기능성품종개발에따른고부가가치상품의개발이요구되고있다. 향미의향기성분은심리안정등일부기능성관련보고도되어있으나기능성성분보다는기호성에따른소비형태로분류되고있다. 따라서향미는최근까지도일부소비층에서소비되는매우제한적인소비형태를유지하고있었으나, 해외근로자와다문화가족을중심으로향미벼의소비가크게늘고있는추세에있다. 국내의경우주로찰벼및기능성이복합화된흑미형태의향미품종들이개발보급되어있으나, 향의구분이불분명하고향기정도가약한품종이대부분이다. 쌀소비측면에서는기능성보다가공용품종의활용성이매우크다. 현재쌀가공용으로육성보급되고있는품종들의경우수입쌀등에비해가격이높아경쟁력이낮고, 특히다양한쌀가공품을위한가공적성이낮아활용이매우낮은실정이다. 따라서쌀소비촉진을위해서는기존의정체된유색미및양조 떡등전통식품을벗어난새로운쌀소비시장창출을위한다양한기능성품종개발과함께원료곡의가격경쟁력이높은초다수성품종의개발이절실한상황이다. 또한코팅기술을이용한고부가가치기능성쌀과함께다양한형태의전분을이용한산업용소재로의활용등특수미의활용성을높이기위한연구를통하여밥쌀중심구조에서벗어나시장창출이필요하다. * 주저자 : Tel , hy4779@korea.kr 17

74 OD-02 Sun-Hyung Lim Flavonoids belong to polyphenolic secondary metabolic compounds ubiquitously found in the plant kingdom. They play important roles not only in plant biological processes including accumulation of pigments in flowers, fruits and seeds for pollination and seed dispersal and protection of UV light, but also in human health including inhibition of cell proliferation and antioxidant properties. For these reasons, flavonoids metabolism have received increasing attention for breeders and researchers. For the past decades, many researchers tried to modify the flower colors through the modification of flavonoid biosynthesis. It has been reported the various strategies and approaches for successful metabolic engineering of flavonoids in plants. It requires an elucidation of structural and regulatory genes for the biosynthetic pathway and a detailed understanding of metabolic flux through competitive pathways. From this point of view, I will present our recent results divided into three parts; (I) modification of flower color by metabolic engineering; (II) elucidation of the regulatory mechanism controlling the onset of the pathways; and (III) identification of genes involved in the desired metabolites. *Corresponding Author: Tel , limsh2@korea.kr OD-03 Jin-Seoung Cho 1, Min-Ha Kim 1, Hyung-Woo Jeon 1, Eung-Jun Park 2, Hyoshin Lee 2, Young-Im Choi 2 Jae-Heung Ko 1* 1 2 Research of woody biomass production has increased as a potential renewable resource for biofuel. During past five years, we performed a project called development of poplar super clones supported by Korea Forest Service. Here, research products of the project will be shared and discussed. Firstly, a functional genomics study on the entire family of poplar MYB transcription factor will be introduced. Poplar, a model woody plant, has a total of 212 R2R3-MYBs, which is much larger than that of Arabidopsis (159 members). Expansion of poplar MYB family suggests specific components of poplar life history, such as secondary growth (e.g., wood formation), perennial growth, and reproductive development. As a first step towards understanding of functional role of MYB family in woody perennials, the entire MYB family members of P. trichocarpa were cloned in overexpression and suppression constructs. Through the phenotype-based screening of both transgenic poplar and Arabidopsis populations, we could identify many of plants having altered secondary growth or secondary cell wall formation. Secondly for the targeted approach, we utilized plant hormone Gibberellin (GA) and secondary wall forming MYB regulators as well. We produced transgenic Arabidopsis plants and Poplar expressing GA20-oxidase isolated from Pinus densiflora (PdGA20ox) under the control of either 35S or developing xylem-specific promoter, respectively. Both transgenic Arabidopsis plants and Poplar exhibit an accelerated stem growth and results in a massive increase of biomass compared to control plants. Finally, a novel approach for biotechnological improvement of the quantity and quality of woody biomass with minimizing undesirable growth penalties will be discussed. Our results demonstrate that the controlled production of GAs and secondary wall regulating MYB TF through a DX promoter can be utilized as an efficient biotechnological tool for producing enhanced plant biomass without undesirable side effects. This work was funded by the Korea Forest Service (S111213L080110). *Corresponding Author: Tel , jhko@khu.ac.kr 18

75 OD-04 Seung il Yoo 1, Hwayong Lee 2, Kesavan Markkandan 1, Suyun Moon 2, Junsu Ko 1, Hojin Ryu 2, Chang Pyo Hong 1* 1 2 Lentinula edodes is one of the most popular edible mushrooms in the world and contains useful medicinal components such as lentinan, beta-glucans and many metabolites. Light-induced brown film formation on vegetative mycelial tissues in L. edodes is one of the most important processes for ensuring the quantity and quality of this edible mushroom. However, the molecular mechanisms underlying this critical developmental process is still unclear especially at the level of transcriptome. In this study, we newly identified a proper L. edodes mutant, chamaram cultivar, showing abnormal functional brown film formation. We performed global genome wide transcriptomic analysis in the three different types of brown film mycelial tissues including white, brown and abnormal dark yellow partial brown film states. Comparative transcriptome analysis between normal browning and whitening status, that consisting of 1490 differentially expressed genes, revealed the significant changes of genes associated with hydrolase activity and carbohydrate metabolic process for browning, and proteolysis for whitening. Changes of transcription factors were also identified. Interestingly, genes encoding glucans, especially exg family, belonging to glycoside hydrolases were significantly changed, suggesting the involvement in lentinan biosynthesis. Partially browning strain showed expression pattern distinct from browning and whitening strains, with significant association with flavin adenine dinucleotide binding, indicating lack of catalysis difficult redox reactions. Our data will provide the understanding for browning formation in L. edodes and a foundation for future breeding. *Corresponding Author: Tel , changpyo.hong@theragenetex.com OD-05 Cheryl Adeva 1, Yun-A Jeon 1, Kyu-Chan Shim 1, Luong Ngoc Ha 1, Woo-Jin Kim 1, Jun-Hyun Cho 2, Sun Ha Kim 1, Yun Gyeong Lee 1, Hyun-Sook Lee 1, Changsoo Kim 1, and Sang-Nag Ahn 1* 1 2 Genotyping-by-sequencing (GBS) is the latest approach that facilitates and allows efficient and high-density SNP discovery and genotyping; thereby, can be used in several genetic studies like QTL mapping in a variety of crop species. Starch, the primary component of food crops for human diet, is normally digested in the small intestine. However, a proportion of starch in cereals is resistant to digestion and escapes degradation in the stomach and small intestine. This starch is known as resistant starch (RS). A cereal grain higher in amylose content (AC) is considered a good source of RS. Hence, cereal grains higher in RS are said to be an important contributor to improve gastro-intestinal health. In this study, 92 recombinant inbred lines (RILs) derived from a cross between two japonica rice cultivars, Dodam (high in RS) and Hwayeong (a non-waxy cultivar) were evaluated for mapping QTL for agronomic traits using GBS approach. One major QTL was identified and mapped on chromosome 2 resulting to an increased AC and RS which was similar to previous report. QTLs were also identified that are associated with the early heading trait and micronutrients. Thus, these results implied that using a high-density of SNP markers for QTL mapping for agronomic traits through GBS offers substantiation for various applications in rice research and breeding. Acknowledgement: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2017R1A2B ) *Corresponding Author: Tel , ahnsn@cnu.ac.kr 19

76 OD-06 Sae Hyun Lee 1, Hong-Seob Yu 1, Ho Jun Joh 1, Hyun-Seung Park 1, Jee Young Park 1, Tae-Jin Yang 1 1 Cynanchum wilfordii has long been regarded as a medicinal plant in Korea. The cultivation area and annual production have increased due to its various pharmacological effects, especially for menopause. However, C. wilfordii is morphologically similar with C. auriculatum, which may cause misidentification or mal-utilization in the market. Therefore it is necessary to develop discrimination methods between two species. Most of the markers are designed from chloroplast genome sequences for the identification of plant species. For Cynanchum species, several markers have been developed for species identification but they often showed confusable result which might be caused from intra-specific variations due to the natural diversity. Therefore, to develop more stable authentication markers, we assembled complete chloroplast genome and 45s rdna of four C. wilfordii using NGS data with dnalcw method(de novo assembly of low coverage WGS sequence). By comparative analysis, we identified six SNPs and six InDels in the chloroplast genome of four C. wilfordii accessions. Five SNPs were identified in the intergenic region and one SNP was found in the exon region. We also identified six InDels, two in the intergenic region and four in the exon region. Additionally, we filtered out the areas where the NGS reads were mapped heterogeneously. We also identified approximately 34.3% of chloroplast genome sequences were shared in the mitochondrial genome which show higher sequence homology. After excluding these chloroplast-mitochondrial flux genome regions, and ambiguous intra-species polymorphic regions, we identified 818 SNPs between two species. Among them, we developed seven KASP markers for the identification of C. wilfordii and C. auriculatum. The intra-specific variation of the chloroplast genome of C. wilfordii could be used for understanding of natural diversity of this species and developed KASP marker contribute to more accurate and clear authentication of C. wilfordii and C. auriculatum. This research was supported by Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ013238), Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , tjyang@snu.ac.kr 20

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79 PA-01 Seong-Woo Cho 1, Eun-jin Cho 1, Changsoo Kim 2, Youn-Soo Choi 3, Chon-Sik Kang 4, Chul Soo Park 1* Chromosomal karyotype of 26 Korean wheat cultivars were observed to identify diversity of B genome using (AAC)5 and (AAG)5 as simple sequence repeats (SSRs) by fluorescence in situ hybridization (FISH). Two SSR markers as a chromosomal marker are useful to identify B genome with a higher divergence rather than A- and D-genomes. Several chromosomes of A and D genomes were identified by the two SSR markers, and each chromosome of B genome was discriminated by those markers. Also, FISH revealed the same signal pattern(s) of the SSRs on satellite of chromosome 1B of B genome. However, in euchromatic region on the short arm of chromosome 1B, the signal patterns of the SSRs were different among the Korean wheat cultivars. Especially, Olgeuru had low density of (AAG)5 signal of chromosome 1B rather than the other Korean wheat cultivars, while Joeun showed wide distribution of (AAG)5 signal on the near centromeric region of chromosome 1B rather than the others. Johan showed low density of (AAC)5 signal of chromosome 1B rather than Keumkang as a control. Based on this study, it can be expected that other SSR markers such as (AC)8, (AG)12, and (CAG)5 are useful to identify other chromosomes of A and D genomes. Hence, we suggest that chromosomal markers using SSRs are useful to investigate and compare karyotype of Korean wheat chromosomes for Korean wheat breeding program. Acknowledgement: This work is supported by the Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ ), Rural Development Administration, Republic of Korea *Corresponding Author: Tel , pcs89@jbnu.ac.kr PA-02 Seong-Woo Cho 1, Taek-Gyu Kang 1, Seong-Wook Kang 1, Jung-Min Park 1, Chon-Sik Kang 2, Chul Soo Park 1* 1 2 Twenty-eight Korean wheats were evaluated tiller number according to STS markers for agronomic traits and processing quality genes and SSR markers related to tiller number for improvement of tiller number. For vernalization (Vrn), the mean of tiller number per m 2 (TN) of 23 Korean wheats harboring vrn-b1 allele was higher than it of the other Korean wheats harboring Vrn-B1a, and effective alleles (Ne), Shannon s information index (I), and Nei s gene diversity (h) were 1.41, 0.47, and 0.29, respectively at VRN-B1. At VRN-D1, TN of 17 Korean wheats carrying Vrn-D1a was higher than it of the other Korean wheats carrying vrn-d1, and Ne, I, and h were 1.91, 0.67, and 0,48, respectively. For photoperiod (Ppd), TN of 24 Korean wheats carrying Ppd-B1b was lower than the other Korean wheats carrying Ppd-B1a, and Ne, I, and h were 1.32, 0.41, and 0.25, respectively at Ppd-B1. For puroindoline (Pin), TN of 25 Korean wheats carrying Pina-D1a was similar to it of the other Korean wheat carrying Pina-D1b, and Ne, I, and h were 1.24, 0.34, and 0.19, respectively at Pina-D1. At Pinb-D1, TN of 15 Korean wheats carrying Pinb-D1a was lower than it of the other Korean wheats carrying Pinb-D1b, and Ne, I, and h were 1.99, 0.69, and 0.50, respectively. Among Korean wheats, markers related to tiller and fertile tiller inhibition were not contributed to evaluate tiller number. Also, it is possible to evaluate tiller number by six markers. Based on those results, Korean wheats were classified into three groups by unrooted dendrogram. Acknowledgement: This work is supported by the Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ ), Rural Development Administration, Republic of Korea *Corresponding Author: Tel , pcs89@jbnu.ac.kr 23

80 PA-03 Seong-Woo Cho 1, Taek-Gyu Kang 1, Seong-Wook Kang 1, Jung-Min Park 1, Chon-Sik Kang 2, Chul Soo Park 1* 1 2 Double haploid population with 104 lines were evaluated tiller number according to STS markers for agronomic traits and processing quality genes and SSR markers related to tiller number for improvement of tiller number. For vernalization (Vrn), the mean of tiller number per m 2 (TN) of 50 double haploid lines harboring vrn-b1 allele was similar to it of the other double haploid lines harboring Vrn-B1a allele, and effective alleles (Ne), Shannon s information index (I), and Nei s gene diversity (h) were 1.41, 0.47, and 0.29, respectively at VRN-B1 locus. At Pinb-D1 locus, TN of the double haploid lines carrying Pinb-D1a allele was similar to it of the other double haploid lines carrying Pinb-D1b allele, and Ne, I, and h were 1.97, 0.69, and 0.49, respectively. All lines of the double haploid population were harboring vrn-a1, vrn-d1, vrn-b3, Ppd-A1b, Ppd-B1a, Ppd-D1a, and Pina-D1a alleles. Most markers related to tiller number divided the double haploid population as two groups. TN of each group was not significantly different. Based on those results, the double haploid population was classified into two types. Acknowledgement: This work is supported by the Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ ), Rural Development Administration, Republic of Korea *Corresponding Author: Tel , pcs89@jbnu.ac.kr PA-04 Seong-Woo Cho 1, Taek-Gyu Kang 1, Seong-Wook Kang 1, Jung-Min Park 1, Chon-Sik Kang 2, Chul Soo Park 1* 1 2 Genetic F8 population with 157 lines were evaluated tiller number according to STS markers for agronomic traits and processing quality genes and SSR markers related to tiller number for improvement of tiller number. For vernalization (Vrn), the mean of tiller number per m 2 (TN) of 149 F8 lines harboring vrn-a1 allele was similar to it of the other F8 lines harboring vrn-a1a allele, and effective alleles (Ne), Shannon s information index (I), and Nei s gene diversity (h) were 1.11, 0.20, and 0.10, respectively at VRN-A1. At VRN-D1 locus, TN of 150 F8 lines were harboring vrn-d1a allele was similar to it of the other F8 lines harboring vrn-d1 allele, and Ne, I, and h were 1.09, 0.18, and 0.09, respectively. For photoperiod (Ppd), TN of 125 F8 lines were harboring Ppd-D1a allele was not significantly different from it of the other F8 lines harboring Ppd-D1b allele, and Ne, I, and h were 1.48, 0.51, and 0.32, respectively. For puroindolines (Pin), TN of 98 F8 lines were harboring Pinb-D1b allele was similar to it of the other F8 lines harboring Pinb-D1a allele, and Ne, I, and h were 1.88, 0.66, and 0.47, respectively. Among SSR markers related to tiller number, 5 SSR markers divided the F8 population as two types. TN of each type according to the 5 markers was not significantly different. Unrooted dendrogram revealed many branches with may subclasses. Acknowledgement: This work is supported by the Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ ), Rural Development Administration, Republic of Korea *Corresponding Author: Tel , pcs89@jbnu.ac.kr 24

81 PA-05 Taewook Kim 1, Sang-Yoon Shin 1,2, Minsu Park 1, Jemin Kim 1 and Chanseok Shin 1,2* 1 2 Pre-harvest sprouting (PHS) is one of the economic problems associated with seed dormancy. It seriously affects crop production in the regions under humid climate and causes a sharp reduction in grain quality. Therefore, understanding the processes controlling dormancy is crucial to overcome PHS problem. To unveil how seed dormancy is regulated in Arabidopsis, we compared two different ecotypes of Arabidopsis, Col-0 (low dormancy) and Cvi-0 (high dormancy) under three different stages of seed development. We investigated transcriptome-wide responses of small RNAs during breaking seed dormancy. We found that differential expression of well-known marker genes associated with processes of dormancy and germination between Col-0 and Cvi-0 (dog1, abi3, fus3, cyp707a2, ga3ox1). Also, we identified approximately 1,500 small RNAs and some of them located near genes which are related to dormancy. This study might provide a foundation for understanding dynamics of transcriptome during breaking seed dormancy. This work was supported by the Next-Generation BioGreen 21 Program (No. PJ ), Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , cshin@snu.ac.kr PA-06 Vimalraj Mani, Soyoung Park, Joon-Soo Sim, Su-Yeon Kim, Jaeeun Song and Bum-Soo Hahn * Capillary electrophoresis-mass spectrometry (CE-MS) can be considered a useful analytical technique for the global profiling of polar and charged metabolites in biological samples. In this study, we have established and evaluated for the separation and relative quantitative analysis of cationic polar metabolites in the 46 tomato germplasms. Experimental setting (the CE-MS interface, BGE (background electrolyte) and mass spectrometer) was optimized to afford a good separation of 20 polar metabolites among the 46 tomato germplasms in less than 40 mins. The sheath liquid of 50% (v, v) methanol was delivered through a syringe pump at a flow rate of 4µL/min. The BGE was consisted of 0.25 mol/l formic acid. The identification of each compound to be present in the extracts of 46 tomato germplasms was identified using available information of standard compounds (retention time, accurate mass and enhanced product ions). In this way, totally 20 targeted metabolites were identified with our available standard library as follows: Spermidine, 1,4-Butanediamine, L-2,4-Diaminobutyric acid monohydrochloride, N,N-Dimethylglycine, 4-Aminobutyric acid, Cytidine, Adenosine, 5 -Deoxy-5 -(methylthio) adenosine, L(+)- Isoleucine, L(+)-Lysine, L-Glutamic acid, L-Aspartic Acid, Glutathione reduced, L-pyroglutamic acid, L(-)-Phenylalanine, Tyramine, L-Citrulline, L-Threonine, L(-)- Proline, L-Histidine and L-Pipecolinic acid. Principal component analysis results showed the sum of two components amounted to 66%. In this case, the diversity of metabolites was found L-Histidine, L-Citrulline and Tyramine in the tomato germplasm. Moreover, we proposed CE-MS methods and a successful relative quantification of polar metabolites to understand metabolite diversity within tomato germplasm. Therefore, this approach help us understand a broad range of fields for identifying ionic components in plant germplasm. *Corresponding Author: Tel , bshahn@korea.kr 25

82 PA-07 Jae-Keun Choi 1,3, Kyu Jin Sa 2, Dae Hyun Park 2, Su Eun Lim 2, Si-Hwan Ryu 1, Jong Yeol Park 1, Ki Jin Park 1, Hae-Ik Rhee 3, Ju Kyong Lee 2* The double haploid (DH) technology offers an array of advantages in maize genetics and breeding. In our study, we constructed a maize genetic linkage map using SSR markers and DH population derived from a cross of normal corn (HF1) and normal corn (11S6169). Total of 200 SSR markers were assigned to 10 linkage groups which spanned 1,145.4 cm with an average genetic distance between markers of 5.7 cm. The 68 SSR markers showed Mendelian segregation ratios in the DH population at a 5% significance threshold. A total of 15 quantitative trait loci (QTLs) for plant height (PH), ear height (EH), ear height ratio (ER), leaf length (LL), ear length (EL), setted ear length (SEL), setted ear ratio (SER), ear width (EW), 100 kernel weight (100KW), and cob color (CC) were found in the 121 DH population. These QTLs were mapped to chromosomes (ch.) 1, 2, 3, 4, 5, 7, and 10. Among these QTLs, two QTLs was associated with PH on ch. 4 and 10, one with EH on ch. 10, one with ER on ch. 5, two with LL on ch. 2 and 7, two with EL on ch. 2 and 5, two with SEL on ch. 7 and 10, two with SER on ch. 2 and 4, one with EW on ch. 3, one with 100KW on ch. 7, and one QTL was related to CC on ch. 1. We found that four QTLs (qel5, qeh10, qew3, qcc1) were major QTLs based on over 15% for phenotypic variation. These new QTLs identified by the present study could provide as molecular markers as possible for selecting important agronomic traits in maize. The results of this study may help to improve the detection and characterization of agronomic traits and provide great opportunities for maize breeder and researchers using DH population in maize breeding program. *Corresponding Author: Tel , jukyonglee@kangwon.ac.kr PA-08 Kyu Jin Sa 1, Ik-Young Choi 2, Kyong-Cheul Park 2, Ju Kyong Lee 1* 1 2 In this study, 40 new simple sequence repeat (SSR) primer sets were developed from RNA sequences using transcriptome analysis. These new SSR markers were applied to analyze the diversity, relationships, and population structure among 35 accessions of the two cultivated types of Perilla crop and their weedy types. A total of 220 alleles were identified at all loci, with an average of 5.5 and a range between 2 and 10 alleles per locus. The MAF (major allele frequency) per locus varied from to 0.943, with an average of The average polymorphic information content (PIC) value was 0.603, ranging from to Based on population structure analysis, all accessions were divided into three groups: Group I, Group II and the admixed group. The GD of each locus for accessions of cultivated var. frutescens, weedy var. frutescens, cultivated var. crispa, and weedy var. crispa were 0.415, 0.606, 0.308, and 0.480, respectively. Both weedy accessions exhibited higher GD and PIC values than their cultivated types in East Asia. In conclusion, the new SSR primers of Perilla species reported in this study may provide potential genetic markers for population genetics to enhance our understanding of the genetic diversity, genetic relationship and population structure of Perilla crop in East Asia. In addition, new Perilla SSR primers developed from RNA-seq can be used in the future for cultivar identification, conservation of Perilla germplasm resources, genome mapping and tagging of important genes/qtls for Perilla breeding programs. *Corresponding Author: Tel , jukyonglee@kangwon.ac.kr 26

83 PA-09 안억근 1*, 원용재 1, 홍하철 1, 박향미 1, 정국현 1, 현웅조 1, 장재기 2, 이정희 1, 이점호 1, 이상복 1, 정응기 1, 서정필 년국내쌀생산량은 397 만톤, 일인당쌀소비량이 61.8 kg로생산과소비의구조적수급불균형현상이지속되고있다. 이에정부는쌀수급조절을위해 2018 년부터 2 년간한시적으로 논타작물재배지원사업 을추진하고있다. 사료용벼는논의형상과기능을유지하면서이러한수급불균형을해결할수있는좋은수단임에도불구하고현시점에서는밥쌀용과비교하여소득이낮아정부보조금없이는농가현장에서재배를기피하는경향이있다. 이에수량성, 내병충성향상등을통한경제적가치및재배안정성을올릴필요가있다. 이번에육성한사료용벼 미우 는도열병, 흰잎마름병 ( 균계 K1, K2, K3, K3a), 줄무늬잎마름병, 벼멸구및애멸구에저항성이있어병충해방제에들이는노력및비용을절감할수있어친환경안전조사료생산이가능할뿐만아니라농업경영비도절감할수있다. 미우 의지상부건물수량은중부평야, 영남평야및호남평야지 4 개소에서 3 년간시험한결과평균 19.9 톤 /ha 으로 녹양 대비높은수량을나타내경제적가치도높일수있을것으로기대된다. 더구나가축이소화흡수할수있는사료의영양가지표인가소화양분총량 (Total Digestible Nutrients, TDN) 도 70.7% 로일반사료작물과비교하여떨어지지않아사료적가치가높다고판단된다. 이처럼복합내병충성, 높은지상부건물수량및가소화양분을보유한 미우 는밥쌀용과비교해거의동등한수준까지경제적가치를올릴수있을것으로기대되어정부정책과제인쌀생산조정을통한수급조절에일익을담당할것이다. * 사사 : 본연구는농촌진흥청연구사업 ( 세부과제명 : 사료용벼유용형질도입계통육성, 세부과제번호 : PJ ) 의지원에의해이루어진것임. * 교신저자 : Tel , okahn@korea.kr PA-10 Joon Ki Hong 1, Eun Jung Suh 1, Hye-Jin Yoon, Jihee Park, Yeon-Hee Lee 1* 1 GROWTH-REGULATING FACTOR (GRF) genes encode plant-specific transcription factors, and play critical roles in regulating the growth and development of lateral organs. In order to explore the agricultural potential of Brassica rapa GRF genes (BrGRFs), we constructed two BrGRF-overexpressing B. napus plants (BrGRF3-1OX and -9OX). BrGRF3-1OX and 9OX developed larger cotyledons, leaves, and seeds than the wild type. The increases in size of these organs were due to increases in cell number, but not due to cell size. RT-PCR analysis revealed that BrGRFs regulated expression of a wide range of genes that are involved in gibberellin-, auxin-, cell division-related growth processes. Taken together, our data indicate that BrGRFs act as positive regulators of B. napus growth, thus raising the possibility that they may serve as a useful genetic source for crop improvement with respect to organ size and seed production. *Corresponding Author: Tel , yhl2222@korea.kr 27

84 PA-11 Ye-Ji Lee 1,4, Eun-Ju Byeon 1, Kyeong-Seong Cheon 1, Hyeonso Ji 2, Gang-Seob Lee 3, Ung-Han Yoon 1, Nam-In Hyung 4, Tae-Ho Kim 1* Rice (Oryza sativa L.) is one of staple food in the world, especially in Asia. As the population of world increase, food security is important because consumption is gradually increasing. Therefore, the development of new varieties with high productivity is a major goal in rice breeding. In this study, yield-related phenotypes and quantitative trait loci (QTLs) were analyzed using the recombinant inbred lines derived from 'Milyang23' and 'Gihobyeo' (MGRILs). In phenotype investigation, normal distributions were shown in all 11 traits; flag leaf length (FL), culm length (CL), plant height (PH), 100 grain weight (100GW), panicle length (PL), panicle number (PN), spikelet number per panicle (SNPP), spikelet density (SD), grain numbers per plant (GNP), grain weight per panicle (GWP) and grain density (GD). Also, significant difference was mostly shown between 10 traits excepted 100GW. For QTL analysis, newly developed 48 CAPS markers and previous 3,202 SNP and PCR-based markers were integrated to construct high-resolution genetic map. Total genetic distance and an average of marker density were 2,628cM and 0.82cM/marker, respectively. When the genetic map was applied to detect QTLs on yield-related traits, a total of 56 QTLs were identified. Among these QTLs, 7 QTLs were detected from both SNPP and FL, and 16 QTLs were detected from chromosome 1, which mostly included the high LOD value ( 10). In the future, it will be possible to narrow down QTL region and identify genes related to yield by map-based cloning. Furthermore, QTLs information of agriculturally various traits in this study will be used for rice breeding. *Corresponding Author: Tel , thkim@rda.go.kr PA-12 Chang-Ho Ahn 1*, Hyeong-Soo Park 2, Yong-Eui Choi The genus Thuja, a member of the Cupressaceae family, comprises five species in the world. Among them, Korean Arbor-vitae (KAV; Thuja koraiensis Nakai) only occurs in the northeast China and in high mountains over the Korea. As the warming due to climate change has progressed in recent years, natural populations of KAV have been declined. Thus, KAV is designated as a rare and endangered tree species in Korea. Cryopreservation is one of the promising conservation technology for long-term storage of plant cells and tissues. There has not been any study on somatic embryogenesis and cryopreservation system in the genus Thuja until now. Here, we established an in vitro propagation and cryopreservation system for KAV via somatic embryogenesis. Whole megagametophytes with zygotic embryos from immature cones were used as initial explants and cultured on initiation medium. The initiation frequency was about 23.7% although we could not observe the stages of zygotic embryo development due to limited seed resources. The frequency of somatic embryo formation from both non-cryopreserved and cryopreserved cell lines was also tested. There were no statistical differences on the production of somatic embryos between non-cryopreserved and cryopreserved cells (P = ). We also investigated the effect of cyopreservation on genetic fidelity of the plantlets regenerated from non-cryopreserved and cryopreserved embryogenic cell lines. From ISSR analysis, there was no genetic instability in the regenerated plantlets from cryopreserved embryogenic cell lines. The embryogenesis and cryopreservation systems described here have the potential to contribute the conservation and clonal propagation of KAV germplasm. *Corresponding Author: Tel , dosanahn@gmail.com 28

85 PA-13 김성용 1*, 김태균 1, 강형식 1, 홍순영 1, 송승운 1 1 제주지역의감자재배역사는 1950 ~ 60 년대 200 ~ 300ha 수준에서 1980 년대부터급격히증가하였고 1990 년대말 7,000ha 를상회하여전국재배면적의 30% 를점유하고, 생산액이 1,000 억원에이르는중요한소득작목이었다. 그러나제주주재배품종 대지 가 90% 이상계속적인연작재배로더뎅이병발생등문제점이발생하면서재배면적이 2005 년 6,174ha 에서계속감소되어 2016 년 1,636ha 까지감소하였다. 이러한 대지 품종을대처하기위하여제주특별자치도농업기술원에서는더뎅이병저항성등다양한품종육성을추진한결과맛이있고더뎅이병에강한신품종 탐나 를육성하였다. 탐나 의지상부형태는반직립형이며, 경장은 대지 에비하여크고, 화색은흰색으로꽃이많이피고, 숙기는만생종으로 대지 에비하여늦은편이다. 괴경모양은원형이고, 표피는매끄러우며, 엷은담황색을갖고있으며육색은흰색이다. 눈깊이가얕아소비자들이선호하는품종으로재배기간이충분하면상품수량이많은편이다. 탐나 의휴면기간은 20 저장시 일에 90% 이상이타파되어 2 기작재배를하는제주지역및남부지방에적당하다. 또한 탐나 는감자더뎅이병발생이제주주품종 대지 에비하여저항성을가지고있어재배농가들이선호하는품종이다. 탐나 의수량은지역적응시험결과대비품종 대지 와비슷한경향을보였다. * 교신저자 : T , F ksr2202@korea.kr PA-14 박노봉 1*, 남민희 1, 김상열 2, 여운상 2, 이지윤 2, 권오덕 1, 박동수 2, 이종희 2, 조준현 2, 송유천 밥맛에따른소비자기호성변화에대응하기위해국립식량과학원영덕출장소에서는밥맛이우수한최고품질벼품종을육성하고자노력한결과, 우리나라영남평야지와동해안냉조풍지에알맞은밥맛이매우우수한벼 새칠보 를개발하였다. 모본으로재배안전성이뛰어난 계화 26 호 를, 부본으로는최고품질 영덕 44 호 ( 칠보 ) 를 2005 년하계에인공교배하여 2005/2006 년동계온실에서 17 개체의 F 1 식물체를양성, YR25952 의교배번호를부여하였다 년하계포장에전개한 F 2 집단을포장과실내선발하여 2007 년하계에 F 3 세대 28 계통을육성하였다. 이후 F 4 F 6 세대를계통육종법으로전개하면서 2011 년예비선발시험을실시하여미질특성이우수하고병해와재해에도안정적인계통을선발하였다 년생산력검정예비시험, 년생산력검정본시험을실시한후중생이면서우량계통인 YR 을선발하여 영덕 61 호 로명명하였다 년부터 3 년간지역적응시험을실시한결과, 특히밥맛이매우우수한것으로평가되어 2016 년 12 월농작물직무육성신품종선정위원회에서 새칠보 로명명하게되었다. 새칠보 는보통기재배시출수기는 8 월 14 일로중만생종이며, 간장은 65cm 로단간이면서반직립초형이다. 탈립은잘되지않고이삭추출은양호하며까락이거의없다. 화성벼 보다주당수수는 2 개, 수당립수는 14 개더많으며, 현미천립중은 22.4g 으로다소가볍다. 쌀알이맑고투명하며, 밥맛은 화성벼 보다매우좋다. 또한불시출수는안되는편이고, 위조현상에강하고성숙기엽노화가느린편이며, 내냉성은 화성벼 보다약한중약정도이다. 잎도열병과이삭도열병에는약한저항성을보였고, 줄무늬잎마름병, 흰잎마름병 (K 1) 에는강하나오갈병, 검은줄오갈병에는약하고, 벼멸구등충해에는감수성이다. 쌀수량은지역적응시험보통기재배 7 개소에서 6.13T/ha 로 화성벼 보다 9% 증수되었으며적응지역인영남평야지, 남부중산간지, 동남부해안지에보급하게되었다. * 교신저자 : Tel , parknb@korea.kr 29

86 PA-15 구다은 1*, 주혜준 1, 권용락 1 1 낙엽송은결실주기가긴특성을가지므로조림수요를충족하기위해서는종자의활력저하를최소로하는저장조건을갖추는것이중요하다. 낙엽송종자는저장성이좋은종자로알려져있으나조림수요가꾸준히높은데비해결실예측이어려워종자공급에차질을빚고있으며, 구체적인저장조건과수명에대한자료는부족한실정이다. 따라서본연구는낙엽송종자의장기저장및안정적공급을위해저장기간별최적저장조건을찾아저장종자관리에이용하고자수행되었다. 이를위하여낙엽송종자를저장온도 5 조건 (-18, -4, 4, 15, 25 ), 종자함수율 3 조건 (5%, 10%, 15%) 을조합하여총 15 가지처리를하였으며, 이를각각 6 개월, 18 개월, 30 개월후에발아실험하였다. 발아실험결과를바탕으로저장기간별가장적합한저장조건을탐색하기위해데이터에이차회귀모형을적합하여최적반응값과요인수준을예측하는통계분석법인반응표면분석법 (RSM) 을이용하여저장기간별로최고발아율을보이는저장조건 ( 저장온도, 종자함수율 ) 을예측하였다. 분석결과최고발아율을보이는저장온도, 종자함수율은각각 6 개월저장종자에서 6.43, 9.83%, 18 개월저장종자에서 8.01, 7.36%, 30 개월저장종자에서 13.95, 9.76% 로나타났다. 본결과로미루어볼때낙엽송종자의 2 년이하단기저장의경우일반적인종자저장온도인 4 이상의온도조건에서도종자의활력이크게떨어지지않고, 저장기간이 2 년이상일경우약 14 의저온조건에서종자함수율 10% 내외로저장하는것이종자의활력유지에유리한것으로보인다. * 교신저자 : Tel , daeungu@korea.kr PA-16 구다은 1*, 페레즈무카사 2, 조병관 2, 주혜준 1, 권용락 본실험은초분광영상분석을수목종자에적용하기위해시험적으로선별된편백종자의선별결과를검증하고자수행되었다. 초분광영상은물체가광에반응하여방출하는파장을 slit 과분광장치를이용해수백개의구역 ( 밴드 ) 로세분하여촬영한영상으로, 초분광영상분석을통한종자의비파괴선별은개별종자의광스펙트럼을분석하여활력종자와퇴화종자를구분하는원리이다. 채종원생산단지별 ( 상효 69, 동홍 80) 로각각약 500 립의편백종자를충남대학교비파괴바이오센싱실험실에서초분광영상분석을이용하여선별한결과, 상효 69 에서생산된종자 37 립, 동홍 80 에서생산된종자 30 립이충실한것으로선별되었다. 선별결과를검증하기위한발아실험결과, 상효 69 의종자에서총 437 립의샘플중선별결과와발아실험결과가일치하는경우는 434 립으로 99.31% 의정확도를보였고, 동홍 80 의종자에서는총 430 립의샘플중 426 립의결과가일치하여 99.07% 의정확도를보였다. 검증실험결과초분광영상분석을통한선별이 99% 이상정확하게이루어졌으며, 조사기간중 7-9 일차에발아가집중되어선별된발아종자의종자세가좋은것으로나타났다. * 교신저자 : Tel , daeungu@korea.kr 30

87 PA-17 원용재 1*, 안억근 1, 정국현 1, 홍하철 1, 현웅조 1, 박향미 1, 윤미라 1, 이정희 2, 서정필 3, 정응기 1, 성낙식 1, 곽지은 1, 오세관 쌀의굳기가약한연질이고쌀배유에밀과같은형태의둥근전분을가지고있으면서건식쌀가루품질특성이개선된신품종 미시루 를개발하였다. 건식쌀가루전용벼 미시루 는 2007 년하계에중만생종이면서현미천립중이 34.8g 인대립으로쌀튀김성과현미튀김정립률이좋은 대립벼 1 호 를모본으로, 밥맛이우수한 일품 의돌연변이로뽀얀멥쌀특성을가져양조용으로산업화에성공한 설갱 을부본으로교배하여대립이면서뽀얀멥쌀의특성을조합하여개발된건식쌀가루전용품종이다. 미시루 의이삭패는시기는 8 월 17 일로중만생종이다. 벼키는 85 cm로 화성 보다작고, 현미천립중은 30.8g 으로 화성 보다 1.5 배정도무거우며, 쌀수량은 566kg/10a 이다. 쓰러짐에강하지만병해충에약하고, 수발아에중정도의반응을보여재배적응지역은중부평야이다. 미시루 는쌀알의굳기가 2,239g 으로대립벼 1 호 4,280g 의 52% 에불과하다. 배유내전분구조가밀과같이둥글어건식쌀가루의입자는평균 65 μm로작고, 손상전분이 5.1% 로낮아쌀가루용쌀로적합하다. 향후 미시루 의내병성, 내재해성을개선하고원료곡의경제성을높일수있도록수향성을향상시킬계획이다. 본연구는농촌진흥청연구사업 ( 세부과제명 : 중부지역적응초다수성쌀가루전용품종육성, 세부과제번호 : PJ ) 의지원에의해이루어진결과임. * 교신저자 : Tel , yjwon@korea.kr PA-18 이지윤 1*, 조준현 1, 이종희 2, 오성환 2, 박노봉 3, 송유천 1, 박병명 1, 오인여 1, 박동수 1, 남민희 3, 오명규 금강 1 호 는가공용원료곡의가격경쟁력향상을위해개발된초다수성벼품종이다 년하계에복합내병성을가지며수량성이높은다산 1 호와초형이직립하여수광태세가좋은금강을각각모본과부본으로인공교배를실시하였다 년하계에 F 1 양성, 2009 년 F 2 집단에서선발된 80 개체를 2010 년하계에 F 3 계통으로전개하였다. 이후 2011 년부터 2012 년까지 2 년간계통육종법에따라세대를진전시켰으며, 2013 년예비선발시험과 2 년간의생산력검정시험을거쳐밀양 307 호의계통명이부여되었다 년부터 2017 년까지 3 년간의지역적응시험결과, 수량성이높고흰잎마름병 (K1 ~ K3a), 줄무늬잎마름병, 도열병저항성등의우수성이인정되어 2017 년농작물직무육성신품종선정위원회에서 금강 1 호 로명명되었다. 금강 1 호 는출수기가 8 월 14 일인중만생종이며, 간장은 74 cm로 다산 과비슷하다. 수장과주당수수는각각은 26 cm와 13 개이며, 수당립수가 142 개로다산보다 25% 많다. 금강 1 호 는도복에강하고수발아가거의되지않는다. 금강 1 호 는쌀수량이 817kg/10a 로우리나라에서개발된벼품종중에서수량성이가장높다. 향후 금강 1 호 에대한다양한가공적성이구명된다면우리나라쌀가공용원료곡의가격경쟁력향상에일조할것으로전망된다. * 교신저자 : Tel , minitia@korea.kr 31

88 PA-19 Junewoo Lee 2*, Ronghui Yang 3, Sonia Philosoph-Hadas 4, Shimon Meir 4, Cai-Zhong Jiang 5, Caren Chang 6, Young-Hoon Park 2, Byoung-Cheorl Kang 1, Mark L. Tucker 3, and Joonyup Kim Abscission, organ separation, plays a crucial role in the life cycle of a plant. Precise and timely regulation of abscission is fundamental to improvement of crop productivity as it influences fruit quality and the timing of harvest. To gain insights into commonalities of abscission processes conserved across the plant kingdom, we examined the transcriptomes for abscission in tomato (flower pedicels), soybean (leaf petioles), and Arabidopsis (floral organs). Comparative analyses of the transcriptome data in three different systems allowed us to acquire unprecedented perspectives that assist in successful separation from the parent plant. We uncovered an early increase in the expression of genes that underlies the synthesis of a waxy-like cuticle, which mirrored the expression pattern for cell wall disassembly genes. Similarly, an abundance of expression for small pathogenesis-related (PR) genes also indicated that these genes are more closely related to the structural changes in the abscission-zone (AZ) than an enzymatic role in pathogen resistance. In addition, sequences and the expression of INFLORESCENCE DEFICIENT IN ABSCISSION(IDA) and IDA-like genes in three different systems were conserved and associated with the abscission processes. To functionally validate the IDA-like gene in tomato, we generated tomato (Solanum lycopersicum, VF36) RNAi lines that target AZ-specific expression of SlIDA1 (SlIDA1 RNAi) and examined the effects of suppression of SlIDA1. Alternatively, we investigated expression for SlIDA-like genes (SlIDA1 to SlIDA5) in pedicel AZ from a variety of natural abscission variants in tomato (i.e., jointless, functionally impaired jointless (knuckle-like AZ),normal joint). Whereas the suppression of SlIDA1 expression did not correlate with the expected delay in pedicel abscission of SlIDA1 RNAi, transcript profiles of SlIDA-like genes in the natural tomato variants indicated that expression of SlIDAs, to some extent, is associated with the formation of AZ. Treatment of ethylene action inhibitor, 1-MCP, in detached fruits and leaves of SlIDA1 RNAi that blocked dark-induced senescence processes further substantiated the previous finding that ethylene is essential in tomato abscission, and the function of SlIDA1 expression is ethylene-dependent in tomato. Functional redundancy and possible divergence of SlIDA-like genes in relationship with ethylene will be discussed. *Corresponding Author: uncleanone15@naver.com 32

89 PA-20 Woon Ji Kim 1, Hokeun Sun 2, Tae-Hwan Jun 3, Man Soo Choi 4, Sungtaeg Kang 5, Soon-Chun Jeong 6, Jung-Kyung Moon 7, Bo-Keun Ha 1* Soybean is a major crop that provides a crucial source of edible protein and oil. Wild soybean (Glycine soja) contains an important genetic variation for improving agronomic traits in cultivated soybeans. In this study, SNPs associated with protein and oil contents were identified using a core collection of wild soybeans genotyped with Affymetrix Axiom 180k SoyaSNP genotyping array by RDA (Rural Development Administration), Korea. Seed compositions of 445 wild soybean accessions were evaluated at Gwangju in 2016 and The content of the protein ranged from to 55.83% with the mean of 48.97% and the content of the oil ranged from 4.03 to 12.82% with the mean of 7.35%. The protein content negative correlated with oil content (P 0.01). The elastic-net method was used for the multiple-snp analysis. Based on the selection probability of 0.5, 28 SNP markers associated with protein content were identified across soybean chromosomes, while 64 SNP markers associated with oil content were discovered. This study provides resources for genomics-enabled improvements in soybean breeding. *Corresponding Author: Tel , bkha@jnu.ac.kr PA-21 Woon Ji Kim 1, Hokeun Sun 2, Tae-Hwan Jun 3, Man Soo Choi 4, Sungtaeg Kang 5, Soon-Chun Jeong 6, Jung-Kyung Moon 7, Bo-Keun Ha 1* Soybeans are one of the most important food crops because they contain all of essential amino acids. In this study, we identified genome-wide SNP markers associated with amino acid content in core collection of wild soybeans genotyped with Affymetrix Axiom 180k SoyaSNP array A total of 375 wild soybean accessions were used to determine amino acid content with amino acid auto-analyzer. The contents of total amino acids ranged from 33,843 to 50,819 (mg/100g), and all amino acids was positively correlated with each other (P 0.05). As a result of SNP estimation using elastic-net method, total of 59 SNPs associated with amino acids content were identified across soybean chromosomes. The identification of SNP markers associated with amino acids contents are expected to be helpful for the development of molecular markers that can be used for soybean breeding. *Corresponding Author: Tel , bkha@jnu.ac.kr 33

90 PA-22 손범영 1*, 백성범 1, 김정태 1, 이진석 1, 배환희 1, 고영삼 1, 김선림 1 1 국내조사료안정생산기반을마련하기위해사료품질이우수하고수량이많은사료용옥수수신품종 다청옥 을육성하였다. 다청옥 은 2016 년에농촌진흥청국립식량과학원에서자식계통 KS197 와 KS202 를교잡하여육성한다수성단교잡종이다. 다청옥 의종피색은황색이며입질 ( 립형 ) 은마치종이다 년 1 년간생산력검정시험을거쳐, 2014 년 ~ 2016 년까지 3 년동안 4 지역에서지역적응시험을수행하였다. 그결과우수성이인정되어 2016 년농작물직무육성신품종으로결정되었고 다청옥 으로이름지어졌다. 다청옥 의생육특성중출사일수가 80 일, 간장은 267cm, 착수고율은 51%, 도복은 1.5, 100 주당이삭수는 95 개, 후기녹체성은 2.9 로대비품종인 광평옥 과비슷한특성을가졌다. 이삭길이 (18.6cmm) 는 광평옥 보다길었지만통계적유의성은인정되지않았다. 깨씨무늬병 (5.7) 에는중간정도의저항성이며, 그을음무늬병 (1.0) 에는강한편이다. 조명나방 (6.7) 에는중간정도의저항성을보인다. 다청옥 의잎과줄기의조단백질함량 (7.3%) 은 광평옥 과비슷하나수입종인 P3394 보다많았다. 다청옥 의잎과줄기의사료가치인 NDF(64.2%) 와 ADF(36.3%) 는 광평옥 및 P3394 와유의한차이는없었다. 다청옥 의건물수량은 톤 /ha, TDN 수량은 톤 /ha 로각각 10%, 7% 광평옥 보다많았으나통계적유의한차이는없었다. 채종특성및수량을검토하기위해모본 ( 종자친 ) 과부본 ( 화분친 ) 의재식비율 ( 모본 : 부본 ) 을 4 1 로동시파종하여시험한결과 다청옥 은모본 (KS197) 의출사기와부본 (KS202) 의화분비산시기가잘일치하는품종이며채종수량 (2.46 톤 /ha) 이많았다. 다청옥 은전국적으로재배가가능한품종이다. * 교신저자 : Tel , sonby@korea.kr PA-23 Jeom-Sig Lee *, Jieun Kwak, Mi-Ra Yoon, Choon-Ki Lee, Eun-Yeong Sim, Namgeol Kim, Ha-Cheol Hong Temperate japonica rice (Oryza sativa L.) is usually grown in temperate regions. When grown in tropical areas, most temperate japonica rice plants flower prematurely and do not show sufficient vegetative growth. Fourteen japonica rice varieties and lines adapting to tropical environments were developed in the Philippines (tropical Asia) between 2008 and Their genomes were characterized by genome-wide single nucleotide polymorphism genotyping and their grain yields were examined in the Philippines during the wet and dry seasons and in a high-altitude area of Burundi (tropical Africa). Based on the genotyping, all 14 materials were found to belong to the temperate japonica rice group. Grain yields were more affected by the environment than by the genotypes. Two of the 14 rice materials showed more stable and higher yields than the check varieties across the three environments and one of the two has been released as a commercial variety in the Philippines. Collectively, these results demonstrate that rice plants genetically belonging to the temperate japonica group can be bred to adapt to tropical areas. *Corresponding Author: Tel , leejsyr@korea.kr 34

91 PA-24 Il Hwan Lee, Jinjoong Kim, Donghwan Shim * The chloroplast (cp) genome is useful in the study of phylogenomics, molecular dating, and molecular evolution. Red pine is an evergreen conifer in the genus Pinus and widely distributed worldwide. It is very important for its economic, scientific, and ecological niches in Korea. Here, we report the complete chloroplast genome sequence of Korean red pine (Pinus densiflora). Genome sequencing was achieved by a combination of Oxford Nanopore MinION and Illumina MiSeq technologies. With the rapid development of sequencing technology and the plummeting cost, assembling whole genomes from non-model plants will soon become routine for plant systematists and evolutionary biologists. In the present study, Korean red pine chloroplast genome size was 119,875 base pairs (bp) (LSC 65,654 bp and SSC 53,231 bp). Compared to the chloroplast of other congeneric species (P. sylvestris, P. thunbergii, P. taivanensis and P. tabuliformis), six highly variable intergenic regions (ndhc/psac, rps16/trnq, trnk/rps16, trnl/trnf, trnm/atpe, and trnq/psbk) were also identified. The chloroplast resources generated by the present study will help to elucidate chloroplast evolution within the genus and to resolve phylogenetic relationships within highly complex and reticulated lineages. *Corresponding Author: Tel , shim.donghwan@gmail.com PA-25 Neha Roy Samir *, Yong-Wook Ban, Hana Yu, Kyong Cheul Park and Ik-Young Choi Plant height is an important component of plant architecture, and significantly affects crop breeding practices and yield. We obtained few segregated dwarf soybeans in the populations derived from crossing of G. max var. Peking and G. soja var. IT in F5 RIL population. These could be useful genetic resource for plant breeders, geneticists and biologists. We tried to find differently expressed genes to classify and understand the regulation of genes related to plant growth in a mutant dwarf soybean, which occurred in F5 generation. With the help of Illumina high-throughput platform transcriptomes were generated and compared among normal and dwarf in triplicates. We found that the expressed genes relationship are complexed to the plant growth. There are highly significantly up-/down-regulated genes in the comparing of gene expression in normal and dwarf soybeans. The genes related to disease and stress responsive were found to be up-regulated in dwarf soybean. Such over-expression of disease resistance and other immune responsive genes could be targeted to understand the gene regulation of how the immune genes regulate the response of plant growth. In addition, photosynthesis related genes showed very low expression in dwarf lines. The transcriptome expression and genes classified related plant growth could be useful resource to researchers studying plant growth. Keywords: soybean, growth, dwarf, transcriptome, DEG Acknowledgement: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 2017R1A2B ) *Corresponding Author: choii@kangwon.ac.kr 35

92 PA-26 Young-Jin Kim *, Kyeong-Min Kim, Kyeong-Hoon Kim, Dong-Jin Shin, Jin-Kyeong Cha, Jong-Min Ko At present, domestic wheat has been developed various harvesting methods for labour-saving and high yielding in order to strengthen price competitiveness with imported wheat, but no cultivation method suitable for regional characteristics of the soil has been established. This study was carried out to determine the effect of different seeding methods on wheat yield. Wheat varieties, Keumkang and Jokyung were used, and sown in Nov. 2 nd 2017 at a paddy field in Haman region. Standard fertilizer level was kg (N P 2 O 5 K 2 O)/ha. The seeding methods consisted of 4 plots. According to the sowing method, When drill seeding with Jokyung, the heading was delayed by one day. When no-tillage broad ridge seeding and broad ridge seeding after rotary with Keumkang, the heading was two days earlier. The maturity was almost the same. Broadcast seeding-rotary showed the highest number of spikes per m 2 in Jokyung (888) and Keumkang (851). The tendency was similar to that of the 1,000-seed weight and grain weight (g/l), but they were heaviest in the broad ridge seeding after rotary and lowest in the broadcast seeding-rotary. Dry matter yield of Jokyung by seeding methods of wheat revealed that there was an increase in orders; broadcast seeding-rotary (502kg/10a) > no-tillage broad ridge seeding (394kg/10a) > broad ridge seeding after rotary (358kg/10a) > drill seeding (336kg/10a). The yields of Keumkang were higher in the order of broadcast seeding-rotary (517kg/10a), followed by broad ridge seeding after rotary (429kg/10a), no-tillage broad ridge seeding (367kg/10a) and drill seeding (332kg/10a). The highest yield was observed in broadcast seeding-rotary, which were sown more than the other sowing methods at 20kg/10a, because they were well grown without suffering from the harmful effects due to severe drought in the fall of 2017 and cold conditions during winter. *Corresponding Author: Tel , yjikim@korea.kr PA-27 Gileung Lee, Jeonghwan Seo, Zhuo Jin, Im Ji Hwan, Hee-Jong Koh * Rice hull consist of two bract-like structures, lemma and palea. This is an important organ protecting the seeds from environment, and determining shape and filling of kernel. An optimal size and morphology will be beneficial for seed development, affecting yield and quality. A split-hull (sph) mutant was induced by treating with a chemical mutagen, N-methyl-N-nitrosourea (MNU), on the fertilized egg cells of a japonica rice cultivar Hwaseonchal. The sph mutant showed split hull phenotype, a chasm between lemma and palea, during grain filling period and reduced seed setting rate. Hull splitting initiated 15 days after flowering (DAF), and about 40 percentage of grains showed split-hull phenotype after seed maturation. Genetic segregation analysis indicated that sph mutant phenotype is controlled by single recessive gene. To identify the SPH gene, BSA and fine-mapping was conducted using F2 and F3 population derived from the cross between sph mutant and Milyang 23 ( Tongil -type variety). SPH gene mapped to 113 kb region containing 27 annotated ORFs in chromosome 4. Through sequence analysis of the candidate ORFs, candidate gene for the mutant phenotype was identified. The SPH gene might be involved in the spikelet development. *Corresponding Author: Tel , heejkoh@snu.ac.kr 36

93 PA-28 Sun-geum Jeong 1, Eun Ju Lee 1, Jun Young Choi 1, Mi Jin Kim 1, Min Jung 1, Man-Sung Yang 2, Youn-Sung Kim 1* 1 2 Controlling flowering time is essentially important in crop plants for high agricultural productivity and adaptation to environmental changes. Currently, a growing number of genes related to flowering time have been studied in crop plants. This study was conducted to produce the plants with late-flowering time using A. tumefaciens mediated CRISPR-Cas9 system in chinese cabbage. A. tumefaciens harboring phatc containing genes encoding Cas9, guide RNA of target gene, and hygromycin phosphotransferase was used to infect hypocotyl explants. After 3 weeks, the calli and roots were observed from the explants. The shoots were developed from the calli after 7 weeks. The insertion of transgenes in plantlets was confirmed using PCR analysis. Targeted deep sequencing of transgenic plants revealed the indel at the target site. To obtain seeds, transgenic plants are being cultivated. These results showed that gene editing with CRISPR-Cas9 system in chinese cabbage could be useful for the development of the new valuable varieties. *Corresponding Author: Tel , yskim0907@naver.com PA-29 최창현 1*, 강천식 1, 손재한 1, 김양길 1, 이수경 2, 박종호 1, 박태일 1, 윤영미 1, 김경호 3, 이점호 국내에서밀은높은활용도와소비율에비해자급률이낮기때문에자급률을높이기위한고품질품종육성이시급함에도불구하고, 밀이가지고있는유전체구조는크고복잡하여유전체분석의어려움이크다. 본연구에서는다양한소비자욕구에맞는고품질밀개발을위한기반을조성하기위해, 국내기상환경에적합하면서유전적다양성을가진밀핵심집단의구축을위하여, 본연구팀은전세계 60 여개국가로부터수집한 1,969 점 ( 국내 350 여점, 중국 160 여점, 아프가니스탄 75 점, 미국 155 점, 멕시코 367 점등 ) 의밀유전자원을확보하여생육특성조사를수행하였다. 입모율, 엽색, 초형, 수형, 출수기, 성숙기등의표현형질을조사하였으며, 그외에도복율및이병율 ( 붉은곰팡이병, 녹병, 흰가루병등 ) 등을조사하였다. 현재전체유전자원으로부터국내기상환경에적합한 600 여점을선발하고, 밀품질특성및농업형질관련 functional marker (FM) 를이용하여 genotype 을분석하고있으며, 표현형과 genotype 간의연관관계를분석할예정이다. 아울러대부분의계통으로부터종자를수확하였으며, 앞으로 NIR 및 Seed counter 분석을통해밀가공적성등종자특성을조사할예정이다. 본연구결과는최종 400 여점의밀핵심집단을구축하는데기초자료로이용될것으로기대된다. * 교신저자 : Tel , chchhy@korea.kr 37

94 PA-30 강천식 1*, 손재한 1, 정영근 1, 최창현 1, 김양길 1, 윤영미 1, 박종호 1, 양선목 1, 김경호 2, 박태일 1, 이점호 국내밀재배는주로벼수확후 10 월중순부터파종하여이듬해 6 월모내기전에수확하는답리작재배방식으로진행되어왔다. 답리작재배에적합한숙기가빠른밀품종개발을목표로 2002 년도에조숙단간인수원 272 호 / 올그루의교잡종을모본으로하고, 조숙고품질인금강 / 수원 252 호의교잡종을부본으로인공교배하여 SW02059 조합을육성하였다. 경기도연천에서집단재배후계통을전개하여초형과수형이양호하며내한성이강한계통인 SW02059-B-B-B 는조숙계통으로 2010 년부터 2 개년간생산력검정을거쳐 익산 360 호 로계통명을부여하였다. 그후 2012 년부터 3 개년동안지역적응시험을실시한결과숙기가빠르면서답리작적응다수성이고, 수발아와도복에대한저항성이강하여생산안정성이우수하였다. 밀가루품질에있어서도중력분에적합한단백질과글루텐을함유하고있으며, 밀가루색과국수색택이좋고, 삶은국수의점성과탄성이우수하여 2014 년직무육성신품종선정심의회에서 조중 으로명명하였다. 그후국립종자원의재배심사를거쳐 2018 년종자산업법에따라품종보호등록원부에등록 ( 품종보호제 6970 호 ) 되었다. * 교신저자 : Tel , kcs1209@korea.kr PA-31 강천식 1*, 손재한 1, 정영근 1, 최창현 1, 김양길 1, 윤영미 1, 박종호 1, 양선목 1, 김경호 2, 박태일 1, 이점호 국내밀품종개발은 1970 년이후금강밀등 35 개품종이개발되었으나국수용이대부분이며, 과자용 3 품종, 빵용으로는조경밀 1 품종이개발보급되고있다. 이에농촌진흥청에서는국산밀용도의다양화를위해빵용밀품종개발을목표로 2005 년도에내한성이강하고밀단백질글루텐중 HMW-GS 조성이 5+10 을지니고있는탑동을모본으로하고, 빵용특성이우수한 Klasic 을부본으로인공교배를실시하여 IW 조합을육성하였다. 전라북도남원에서집단재배후에계통을전개하여초형과수형이양호하고내한성이강한계통인 IW B-B-B-6-10 는조숙계통으로 2011 년과 2012 년에 2 년간생산력검정시험에공시한결과생육특성, 생리장해, 내병성및수량성이우수하여 익산 366 호 로계통명을부여하였다 년도부터 3 개년동안전작 4 개소와답리작 4 개소에서지역적응시험을수행한결과, 숙기가빠르면서답리작적응다수성이고, 백립계이면서대립이고, 붉은곰팡이병에중도저항성이었다. 밀가루품질에있어서도제빵용에적합한단백질과글루텐을함유하고있으며밀가루색택이밝고, 식빵의부피가크고부드러워빵가공적성이우수하여 2015 년직무육성신품종선정심의회에서 백강 으로명명하였다. 그후국립종자원의재배심사를거쳐 2018 년종자산업법에따라품종보호등록원부에등록 ( 품종보호제 6966 호 ) 되었다. * 교신저자 : Tel , kcs1209@korea.kr 38

95 PA-32 강천식 1*, 손재한 1, 정영근 1, 최창현 1, 김양길 1, 윤영미 1, 박종호 1, 양선목 1, 김경호 2, 박태일 1, 이점호 최근비만, 당뇨와같은성인병을예방하기위하여흑미, 보리, 콩과같은잡곡류를취반시혼합하고있으며밀도통밀을취반용으로사용하고있다. 현재취반용으로이용되고있는 신미찰 1 호 는아밀로스함량이매우낮은찰밀이지만, 종피색이붉어일부소비자들이기피하기때문에도정을실시하여판매되는단점을가지고있다. 농촌진흥청에서는이러한단점을개선하고취반적성을향상시키기위하여 2006 년도에적립계찰밀인 신미찰 을모본으로하고, 백립계인금강밀을부본으로인공교배를실시하여 IW 조합을육성하였다. 그후 F1 세대를멕시코 CIMMYT 연구소에서반수체배양을실시하여 DH10326 계통을육성하였다. 전북익산에서내한성이강하고초형과수형이양호한 DH10326 은백립계찰밀이면서수량성이우수하여 2008 ~ 2009 년도 2 년간생산력검정에공시하였다. 생산력검정에서생육특성, 생리장해, 내병성, 내도복성, 수발아성및수량구성요소를조사한결과그특성이우수하여 익산 345 호 로계통명을부여하였다 년도부터 3 개년동안전작 3 개소와답리작 3 개소에서지역적응시험을수행한결과도복에대한저항성이강하고백립계이면서수발아중도저항성으로생산안정성이우수하였다. 품질에있어서도취반시흡수성, 퍼짐성및백도가높으며밥알이부드럽고점탄성이높아혼반용으로적합하여 2012 년직무육성신품종선정심의회에서 백찰 로명명하였다. 그후국립종자원의재배심사를거쳐 2018 년종자산업법에따라품종보호등록원부에등록 ( 품종보호제 6972 호 ) 되었다. * 교신저자 : Tel , kcs1209@korea.kr PA-33 박종호 1*, 박태일 1, 김양길 1, 윤영미 1, 강천식 1, 최창현 1, 손재한 1, 한옥규 2, 윤건식 3, 장지선 4, 배정숙 5, 송재기 청보리는곡류를포함한사료맥류로남부지역을중심으로대부분벼 + 보리작부체계로전통적인이모작방식으로재배되어왔다. 특히청보리에함유된곡실은고급육생산에유리하며, 배합사료를줄일수있어수입곡물에의존도가높은국내축산농가의사료비절감에크게기여하고있다. 국립식량과학원에서는우수한청보리품종개발에대한축산농가의요구에부응하여 2005 년에매끈망, 장간, 다얼성이면서내도복성이양호한 우호 를모본으로하고, 장간, 총체적성이높은 영양 을부본으로인공교배하여반매끈망, 다수성이면서, 사일리지품질이우수한 ' 연호 ' 를개발하였다. 연호 는직립초형으로초장은 100cm 로 영양 보다크며, 파성은 IV 이다. 답리작재배시출수기는 4 월 23 일, 황숙기는 5 월 22 일로 영양 보다하루빠르다. 줄기수는m2당 713 개로다얼성이며, 내한성은고휴에서고사주율이 72.0% 영양 보다강하고, 호위축병은나주 (Ⅰ 형 ), 진주 (Ⅳ ) 에서저항성, 익산 (Ⅲ 형 ) 에서중도저항으로 영양 과비슷한내재해성품종이다. 조사료건물수량은전작에서 17.3 톤 /ha 으로 영양 에비해 7%, 답리작에서평균 11.4 톤 /ha 로 영양 에비해 5% 높다. 조사료품질은조단백질함량이 9.3% 로영양보다높았다. 총가소화영양분 (TDN) 은 68.8% 로 영양 에비하여낮았으나, TDN 수량은 883kg/10a 로 영양 보다높았다. 사일리지품질은 ph4.4 로 영양 의 ph4.6 보다낮았다. 젖산함량은 68.2% 로높고초산및낙산함량이낮아사일리지품질이 Ⅰ 등급으로 영양 보다양호한품종이다. 연호 의적응지역은 1 월최저평균기온 -8 이상으로북부산간내륙지방을제외한전국에서재배가가능하다. 연호 는반매끈망으로, 총체다수성을가지면서, 사일리지품질이우수하여금후축산농가들로부터큰호응을얻을것으로기대되며, 금후신품종이용촉진사업을통한종자생산단계를거쳐 2020 년부터농가에보급될예정이다. * 교신저자 : Tel , ark0104@korea.kr 39

96 PA-34 강천식 1*, 손재한 1, 정영근 1, 최창현 1, 김양길 1, 윤영미 1, 박종호 1, 양선목 1, 김경호 2, 박태일 1, 이점호 현재국내밀보급종품종은금강, 조경, 백중이보급되고있으며, 금강밀이전체재배면적이 70% 이상을차지하였으나붉은곰팡이병에약하고, 다른품종에비해수량이적어농가에서재배를점차기피를하고있다. 이에농촌진흥청에서는금강밀의단점인붉은곰팡이병과수발아저항성을증진시키고, 다수확품종개발을목표로 2007 년도에금강밀을모본으로하고, 내재해성이강하고분얼이많아수량구성요소특성이우수한올그루밀을부본으로인공교배를실시하여 IW 조합을육성하였다. 그후 F1 세대를멕시코 CIMMYT 연구소에서반수체배양을실시하여 DH 계통을육성하였다. 전북익산에서내한성이강하고초형과수형이양호한 DH 은수량성이우수하여 2011 ~ 2012 년도 2 년간생산력검정에공시하였다. 생산력검정시험에공시한결과생육특성, 생리장해, 내병성및수량성이우수하여 익산 367 호 로계통명을부여하였다 년도부터 3 개년동안전작 4 개소와답리작 4 개소에서지역적응시험을수행한결과, 숙기가빠르면서답리작적응다수성이고, 수발아와붉은곰팡이병에저항성이강하였다. 밀가루품질에있어서도국수용에적합한단백질과글루텐을함유하고있으며, 밀가루와국수면대색이밝고, 면발이부드럽고쫄깃하여국수가공적성이우수하기때문에 2015 년직무육성신품종선정심의회에서 새금강 으로명명하였다. 그후국립종자원의재배심사를거쳐 2018 년종자산업법에따라품종보호등록원부에등록 ( 품종보호제 6967 호 ) 되었다. * 교신저자 : Tel , kcs1209@korea.kr PA-35 조지홍 1, 최장규 1*, 박영은 1, 임주성 1, 조광수 1, 장동칠 1, 천충기 1, 이영규 1, 강용구 2, 김성용 3, 조만현 우리나라가을감자는남부지방과제주도를중심으로재배되고있으며, 주품종은일본에서도입된대지와국내에서육성한추백이다. 대지는수량성이높고큰감자가많은좋은품종이지만더뎅이병에약하고전분함량과식미가낮은것이문제이다. 추백은숙기가빠른조생종이지만바이러스에약하고전분함량이낮다. 따라서더뎅이병에강하고식미도우수한 2 기작가을감자품종을육성하기위하여 2010 년고운과대관 호를교배하여 수선 을육성하였다. 수선 은중생종으로지상부는반직립성으로자라고꽃은흰색이며장과는거의달리지않는다. 감자괴경은원형으로표피는완숙시거칠거칠한러셋형태를보이며육색은흰색이다. 일반포장과다발포장에서시험하였을때더뎅이병발생이매우적었으며, 잎말림바이러스에도강한특성을보였다. 모자이크바이러스에는중도저항성이며, 감자역병과겹둥근무늬병에대해서도중도저항성을보였다. 수량성은봄재배시 3 지역평균 31.4ton/ha, 가을재배시 28.2ton/ha 로대지와비슷하였다. 그러나생리장해발생이적어상품성있는감자수량은더높았으며, 건물률도봄재배시 21.74%, 가을재배시 19.80% 로높게나타났다. 괴경의휴면기간은대지와비슷한수확후 50~60 일이다. 수선 의적응지역은전라남북도와경상남도의해안지방과제주도지역이다. 수선 은괴경형태가국내소비자기호에맞고더뎅이병에강하여남부지방 2 기작용으로재배면적이확대될것으로기대된다. * 교신저자 : Tel , jhcho0108@korea.kr 40

97 PA-36 김양길 1*, 이미자 1, 김경호 1, 강천식 1, 박종호 1, 박태일 1, 윤영미 1, 한옥규 1, 최진경 2, 배정숙 3, 송재기 4, 손재한 1, 최창현 1, 이점호 최근건강한삶을즐기려는소비자층이늘고이를겨냥한기능성곡물을이용한식품등이인기를얻고있다. 그로인하여기능성분이풍부하고다양한용도의차별화된품종개발이요구되고있다. 따라서이에적합한품종을육성하기위해 1998 년에쓰러짐에잘견디고, 유색, 다수형등특성을가진 긴쌀보리 / 창녕재배 계통을모본으로, 이삭이긴다수형 긴쌀보리 를부본으로여교배하여국내최초로흑색찰성쌀보리 흑보찰 을개발하였다. 흑보찰 은 6 조이며파성이 Ⅳ 인불시출수가안정적인병성쌀보리로이삭의형태는소수형이고, 까락이길며탈부성이좋다. 간장이 79 cm로쓰러짐에잘견디고, 토양전염바이러스병인보리호위축병에대한저항성인품종이다. 성숙기는새찰쌀보리와비슷한 5 월 25 일이며, 천립중은 30.4g 인대립종으로수량성 ( 조곡 ) 은 ha 당 4.02 톤으로새찰쌀보리의 95% 수준이다. 이품종은아밀로스함량이낮은찰성품종으로흡수율, 퍼짐성이좋으며, 출수후 25 일전 후자색발현이되고출수후 30 일전 후에서흑색이발현되는특징을가진품종으로안토시아닌함량이 0.116±0.005 mg/brain g 으로기존품종보다많이함유되어혼반및가공용이용될것으로기대되며, 보급될재배적응지역은 1 월최저평균기온이 6 이상인보리재배지역이다. * 교신저자 : Tel , kim5yk@korea.kr PA-37 서정현 1*, 한원영 1, 김현태 1, 강범규 1, 신상욱 1, 박장환 1, 김홍식 1, 오은영 1, 이병원 2, 윤홍태 2, 최만수 3, 이영훈 3, 곽도연 검정콩은우리나라에서주로주식인쌀과함께밥을지어먹는용도로사용되어왔다. 소비자들은검정콩껍질안의자엽색이녹색인것을선호하며알이굵어무름성이좋은콩을선호한다. 이러한검정콩은일반콩보다높은시장가격을형성하고있어농가의선호도가높다. 청자 5 호 는대립, 녹자엽인검정콩품종개발을목표로하여 밀양 181 호 와 YS1886( 청두 1 호 Tanbaguro) 을 2007 년인공교배하였으며, 계통육종법을통하여 YS2000-2B 을선발하였다. 13 ~ 14 년도에실시한생산력검정시험에서는만숙, 대립, 녹자엽계통으로유망하여 밀양 294 호 의계통명을부여하고 15 ~ 17 년 3 년간전국 7 개소에서지역적응시험을실시하였다. 청자 5 호 는유한신육형, 엽형이난형, 꽃색이백색, 모용색이갈색, 종실은편구형이며검은색종피에자엽색이녹색인고유특성을가지고있다. 성숙기는 10 월 24 일로표준품종인 청자 3 호 에비해 6 일늦은만숙종이며 100 립중이 37.0g 인대립종이다. 지역적응시험에서 청자 5 호 는 청자 3 호 보다불마름병및콩모자이크바이러스에강하였고, 밀식재배시도복에다소약하였으나시험포장과건조기를이용한실내검정에서 청자 3 호 보다꼬투리터짐에매우강한특성을나타냈다. 청자 5 호 의수량성은지역적응시험 7 개소평균 343kg/10a 로 청자 3 호 (265kg/10a) 보다 30% 증수되었다. 청자 5 호 는 청자 3 호 의환경에따른종피의열피 ( 裂皮 ) 현상을없게하고 청자 4 호 의종실크기를크게개량한품종으로서높은수량성과소비자의기호도에적합한종실품위를가지고있어농가소득향상과동시에소비자의기호를만족시킬수있는밥밑용콩으로서널리소비될것으로기대된다. * 교신저자 : Tel , next0501@korea.kr 41

98 PA-38 손지영 *, 공도훈, 김은경, 박현근, 최성태, 안광환, 권진혁 단감의주요육종목표는만생종 부유 에비하여수확이빠르거나, 씨가없거나, 고품질의특성을지니며떫은맛의우려가없는완전단감품종의육성이다. 완전단감은열성형질로완전단감과의교배조합에의해출현될확률이월등히높다. 고품질의완전단감을육성하기위해서는요구형질을지닌고품질의완전단감의종류가많아야하지만완전단감품종의수는제한적이고, 그중부본으로쓰일수있는수꽃이피는완전단감품종수는극히일부이다. 그러므로교배부본으로쓰일고품질의완전단감육성은꼭필요하다. 완전단감이면서수확기가빠르고고품질의특성을지니는수꽃이피는품종을육성하고자본시험을수행하였다. 단연 는 2009 년완전단감 양풍 과 태추 를각각모본과부본으로교배하여얻은수꽃이착생하는완전단감계통이다. 숙기가 10 월 6 일로빠르고, 과중이 147g 으로중 소과에속하지만당도는 17 Brix 로높고, 경도는 40 N 이상으로 태추 18.3 N 에비해육질이단단하고과피가깨끗한특징을지닌다. 태추 를부본으로사용하여육성된계통에서흔히나타나는과피오염이거의없다. 따라서숙기가빠른고품질완전단감육종의중간모본및부본용으로사용가치가높고, 과육이단단하므로중 소과를선호하는수출용단감으로서의효용가능성도있어이계통을선발하였으며, 추후세부특성조사수행후품종보호출원예정이다. * 교신저자 : Tel , jjmm12@korea.kr PA-39 박기진 *, 박종열, 류시환, 최승출, 최재근, 남궁민, 최재근, 최준근 찰옥수수육종은 80 년대중반이후국내재래종의수집을시작으로분리, 교배등의과정을거쳐약 40 년정도개량을지속하였다. 품질과도복등주요형질에서많은성과를거두었고간식또는여름철별미로인기가있어옥수수전체재배면적의약 60% 정도를점유하고있다. 하지만대부분흰색또는검정색품종개발에집중되어다양성이부족한실정이다. 찰옥수수시장을주도하는미백 2 호품종을활용하여보는맛과기능성을높인다양한색의미백 2 호를개발하기위하여품종의양친을각각반복친으로 3 4 회여교잡하였고최종적으로고정된다양한색의 8 계통을선발하였다. 이들여교잡계통과미백 2 호의양친을활용한다면총 10 계통 (HW9 계열 4 계통, HW3 계열 6 계통 ) 으로미백 2 호과유사한다양한색의안토시아닌품종을개발할수있게되었다. 기존의품종은대부분종피아래의호분층에서색깔이발현되고이색깔은수정당시화분유전자의영향으로색이변할수있는특징이있으나새롭게개발한다양한색의계통은안토시아닌함량에따른색의차이가분명하게나타나지만수정당시화분유전자의영향을받지않는종피에서발현되는특성을가지고있다. 따라서호분층의색에영향을줄수있는화분유전자의색유전자가종피색보다진하지않다면재배상의품종간격리가필요없게된다. 그러나종피에발현된안토시아닌계열의색은손에묻어나거나증숙으로쉽게물로용출되어가공또는취식과정에서다른색과분리해야하는특성이있지만진한보라색의경우기존의호분층에침착하는검정색찰옥수수보다 배이상함량이높아기능성으로활용가치가높다. * 교신저자 : Tel , kjp@korea.kr 42

99 PA-40 Kyong Mi Jun 1, Joung Sug Kim 2, Songhwa Chae 2, Yoon-Mok Pahk 1, Gang-Seob Lee 3, Yeon-Ki Kim 2 and Baek Hie Nahm 1,2* With the release of complete genome sequences in 2005, rice has become one of model systems for genomic studies of monocotyledonous plants. Aiming to improve agronomic traits through functional analysis of genes, we generated mutants via the insertion of Tos17, a mobile retrotransposon that is active during tissue culture. We used to two Korean cultivars, Oryza sativa L. ssp. japonica cv. Ilmibyeo (IM) and Baegjinju1ho (BJJ1), representing white and brown rice in the domestic market, respectively. We analyzed 7,608 flanking sequences of newly transposed Tos17 insertions by the flanking adaptor-ligation PCR method. By applying this strategy, 1,672 and 843 mutants (M 2 generation) were produced in IM and BJJ1, respectively. The Tos17 element was preferentially inserted into the genic regions (approximately 70%) of rice chromosomes. Among the 1,533 genes representing 2,515 domestic mutants, 830 genes harbored new mutations but not Nipponbare Tos17 mutants. The phenotypes of the Tos17 insertion lines showed three semi-dwarf mutants and various leaf type mutants, including those with narrow, pale green, and striped leaves at the vegetative stage. At the reproductive stage, 10 lines showed a 117~156% (2.9~3.9 g) increase in the 100-grain weight compared with that of the wild type. This study demonstrates the potential utility of Tos17 mutants for the improvement of agronomic traits using domestic rice cultivars via an efficient tissue culture method with relatively little time and cost. *Corresponding Author: Baek Hie Nahm Tel , bhnahm@gmail.com PA-41 백만기 1*, 박현수 1, 남정권 1, 조영찬 1, 김보경 1, 김기영 2, 김우재 1, 김정주 1, 고재권 1, 신운철 1, 강경호 1, 정지웅 1, 김춘송 1, 정종민 1, 이건미 1, 박슬기 1, 이영복 1, 이점호 예찬 은쌀밥맛이좋고외관미질이깨끗하고병에강하며재배안정성이높은남부지역적응중만생고품질품종개발을목적으로국립식량과학원에서 2010/2011 년동계에밥맛이좋고수량성이높으며내도복성이며복합내병성인호품을모본으로하고중생종으로밥맛이좋고쌀이깨끗하며흰잎마름병 K1, K2, K3, K3a 에강한익산 537 호 ( 해품 ) 를부본으로교배하였다. 인공교배로교배립 60 립을수확하였고이교배립은세대진전을위하여 2011 년하계에국립식량과학원벼육종포장에서 F1 30 개체를재배하였다. 우량품종을조기육성하기위하여 F1 개체에대하여약배양을실시하여 2011/2012 동계에분화된식물체를온실에재배하여임성이정상인 124 개체를수확하였다 년하계에포장에 AC1 세대 124 계통중초형과수량성, 미질, 잎도열병, 흰잎마름병검정을실시하여우수한 93 계통을선발하였다. 이중 HR29177-AC73-2 에대하여 2013 년과 2014 년에계통육성과동시에생산력검정을실시하였고생육특성, 수량성, 내병성 ( 도열병, 흰잎마름병, 줄무늬잎마름병 ), 미질, 밥맛등을조사하여내도복성이며흰잎마름병및줄무늬잎마름병에저항성이고쌀외관품위가우수한 HR29177-AC 계통을선발 익산 583 호 로계통명을부여하였다. 익산 583 호 는 2015 ~ 2017 년보통기재배전국 11 개소, 이모작재배 2 개소에서지역적응시험을실시한결과수량성, 내병성 ( 흰잎마름병, 줄무늬잎마름병 ) 이강하고쌀외관품위와밥맛이좋은계통으로 2017 년 12 월농촌진흥청농작물직무육성신품종선정심의회에서그우수성이인정되어품종명을 예찬 으로명명하였고충남이남평야지및서남부해안지 ( 충남, 전남북, 경남북 ) 에적응하는품종으로보급하게되었다. * 교신저자 : Tel , baekmg@korea.kr 43

100 PA-42 Sangkyu Park 1, Da-Hye Kim 1, Bora Park 1, Jong-Yeol Lee 1, Sun-Hyung Lim 1* 1 The ethylene response factor (ERF) genes that belong to APETALA2/ethylene-responsive element-binding factor (AP2/ERF) superfamily have a variety of functions in the transcriptional regulation of plant growth and development, and responses to biotic and abiotic stresses. We identified an ERF family gene, OsTF1, from rice. Sequence comparison conducted with two sequences of Arabidopsis ERFs classified as IXc subgroup revealed that OsTF1 protein has EDLL activation domain as well as highly conserved AP2 domain. Transgenic rice plants overexpressing OsTF1 (OsTF1-ox) and OsTF1 fused to EAR-repression domain (OsTF1-SRDX) were generated to demonstrate the functional role of OsTF1. The OsTF1-ox lines exhibited reduced height of plant, and this was recovered in the OsTF1-SRDX lines. This suggests that OsTF1 may negatively affect plant growth. However, the grain size of OsTF1-ox lines was found to be enlarged compared to non-transgenic rice. The length and width of the grains of OsTF1-ox lines increased approximately 15% and 5%, respectively, resulting in a significant increase in grain weight. Scannig electron microscpe (SEM) analysis revealed that the number of cells increased in lemma across the longitudinal axis, suggesting that OsTF1 may play a role in the control of grain size in rice by regulating cell proliferation. *Corresponding Author: Tel , limsh2@korea.kr PA-43 강석범 1, 문영일 1, 윤수현 1, 진성범 1* 1 제주도내부지화 [(C. unshiu C. sinensis) C. reticulata] 를포함한감귤류 ( 세또까, 탐나는봉, 청견 ) 들을재배하고있는대부분의농가들은감귤대목으로써탱자 (Poncirus trifoliate) 를주로사용하고있다. 그러나최근이러한탱자대목에접목된부지화와같은감귤류의접목부위에서자근이발생하여착화및착과량이감소하고품질이저하되는문제가발생하고있으나, 정확한자근발생여부를판별하는것은쉽지않다. 따라서, 본연구는이발생된뿌리가탱자인지자근인지를단시간내에정확하게판별할수있는 SCAR 마커를개발하기위하여, SRAP F4/R15 프라이머를가지고착과량이감소한부지화의자근뿌리와정상적인탱자뿌리로부터추출한 DNA 에대하여 PCR 를수행하여자근뿌리에서만특이적으로증폭된산물에대하여염기서열을분석하였다. 또한, SCAR 프라이머의조합은분석한염기서열을기초로하여자근품종에서만특이적으로증폭되도록 9 조합으로제작하였으며이들 9 조합중자근의발생으로문제가되는품종 ( 세또까, 탐나는봉, 청견 ) 에서만특이적으로증폭되는 5 개 (P1, P2, P3, P4 그리고 P6) 의 SCAR 마커를얻었다. 그리고이선발된 SCAR 마커는제주도내농가에서널리재배되고있으며자근발생으로심각한문제가대두되고있는부지화나무 12 주에대하여정확한판별이가능하였다. 따라서, 이선발된 SCAR 마커의개발은자근발생으로인하여문제화되고있는감귤류들을단시간내에정확하게판별할수있으며이를기초로하여개선된재배기술방법을적용함으로써정상적인착과량을갖도록관리체계를개선할수있을것으로기대된다. * 교신저자 : Tel , pfad7@naver.com 44

101 PA-44 Kyung Jun Lee *, Sebastin Raveendar, Jung-Ro Lee, Myoung-Jae Shin, Gyu-Taek Cho, Kyung-Ho Ma, Gi-An Lee Black soybean has been used as a food source in traditional medicines because their seed coats contain natural phenolic compounds. The objective of this study is to reveal the genetic variation on the antioxidant activity present in the 203 Korean black soybean landraces. Antioxidant activity of 203 Korean black soybean landraces was evaluated with DPPH, ABTS, ferric reducing antioxidant power (FRAP), and total phenolic contents (TPC) in 2012 and 2015, respectively. The antioxidant activities assessed by DPPH, ABTS, FRAP, and TPC assays showed wide variation among accessions and years, respectively. Using the relative antioxidant capacity index. we found that the IT had the highest antioxidant activity. In correlation analysis, days to 50% flowering (DF) and 100-seed weight (SW) showed the positive and negative correlations with antioxidant activity, respectively. In clustering analysis, 203 Korean black soybean landraces were classified into five clusters. Among them, cluster I contained 10 accessions with higher antioxidant activities, smaller SW, longer DF and days to maturity than other accessions. It is anticipated that our results will expand the antioxidant activity database and provide information on Korean black soybean landraces which could be valuable for development of new soybean varieties. *Corresponding Author: Tel , lkj5214@korea.kr PA-45 Wang Xiaohan, Kyung Jun Lee, Sebastin Raveendar, Gi-An Lee, Jung-Ro Lee, Myoung-Jae Shin, Seong-Hoon Kim, Gyu-Taek Cho, Kyung-Ho Ma, kyung-min Kim * Aegilops is closely related to Triticum. It is rich in genetic diversity which can be used as a donor material for wheat breeding. There is high ambiguity in the classification of Aegilops. Thus, it is crucial to conduct further investigation on classification of Aegilops. The pollen morphology based on LM quantitative pollen characteristics among 12 species of Aegilops was observed. The pollen parameters in the A. bicornis, A. columnaris and A. juvenalis species showed taxonomic value in discriminant analysis. However, significant difference was not found between and other types of Aegilops. Clustering analysis based on quantitative pollen characteristics showed a correlation with classification based on morphological data. This study provided the basis for interspecific classification among the species in the genus Aegilops. The basis for the interspecific classification of the genus Aegilops was provided. *Corresponding Author: Tel , kkm@knu.ac.kr 45

102 PA-46 Ga-hee Kim, Kyung Jun Lee, Sebastin Raveendar, Gi-An Lee, Jung-Ro Lee, Myoung-Jae Shin, Gyu-Taek Cho, Yoon-Sup So, Kyung-Ho Ma * Korean landrace black soybeans (Glycine Max L.) are rich in antioxidants, minerals, proteins and fats. The fatty components of black soybean include fatty acids and vitamin E, a fat-soluble vitamin. Vitamin E refers to α -tocopherol. Among the eight tocopherol isomers, it has the highest antioxidant activity in the body. α -tocopherols, like other antioxidants, prevent cancer and cardiovascular disease. We cultivated 225 Korean landrace black soybeans, which were collected in 2015 at the National Agrobiodiversity Center. The harvested seeds were re-cultivated in 2017 to determine the α -tocopherol content. The average amount of α -tocopherol was ± mg% and ± mg% in 2015 and 2017, respectively. In 2015, the highest α -tocopherol content, mg%, was found in IT and the lowest amount, 7.8 mg%, was in IT However, IT showed the highest α-tocopherol content, mg%, while IT showed the lowest, mg% in In comparison to the three standard soybean cultivars, Geomjeongkong 1, Cheongjakong 3, Ilpumgeomjeongkong, which contained respective α-tocopherol content of mg%, mg% and 415.5mg%, 9 accessions showed higher content of alpha tocopherol. The highest content was found in IT (968.5mg%), followed by IT (864.2mg%), IT (831.8mg%), IT (779.9mg%), IT (746.8mg%), IT (746.6mg%), IT (744.4mg%), IT (742.1mg%) and IT (727.6mg%). The α-tocopherol content data of Korean landrace black soybeans could contribute to the nutritional evaluation of genetic resources. *Corresponding Author: Tel , khma@korea.kr PA-47 백형진 1, 마경호 1, 윤문섭 1, 곽도연 2, 고지연 2, 최명은 2, 이경준 국제반건조열대작물연구소 (ICRISAT) 는 UN 의국제농업개발연구자문기구 (CGIAR) 산하연구기구로서조, 수수, 기장, 병아리콩, 땅콩등 11 개작물에대한유전자원을보존및관리하는기관으로약 12 만여점의유전자원을보유하고있다. ICRISAT 의자원분양패턴을보면각작물별총보유자원중 80% 이상이분양이되고있어서이상적인유전자원보존및관리의방향을제시하고있다. ICRISAT 에서는모집단의 10% 이하의자원으로전체의 80% 이상의변이를대변할수있는핵심집단과핵심집단의 10% 이하의자원으로구성된미니핵십집단을작성, 활용하고있다. ICRISAT 에서는병아리콩 1,956 자원, 땅콩 1,704 자원, 비둘기콩 1,290 자원, 진주조 2,094 자원, 수수 2,247 자원, 손가락조 622 자원, 조 155 자원수준의핵심집단을작성하여작물육종프로그램에활용중이다. 또한병아리콩 211 자원, 땅콩 184 자원, 비둘기콩 146 자원, 진주조 238 자원, 수수 242 자원으로미니핵심집단 ( 핵심집단의 10% 내외, 전체모집단의 1% 내외 ) 을작성하여육종프로그램에활용중이다. ICRISAT 에서는핵심집단및미니핵심집단을찾기위한평가를지속적으로수행중이며이중 19 세트의핵심집단과 84 세트의미니핵심집단은 20 여개국의연구자들에게분양되었고수량및품질향상, 내재해성도입등의육종프로그램에활용되었다. ICRISAT 는유전자원의특성평가에각작물별특이적으로개발된특성조사표를이용하고있으며사용자가필요로하는유용한특성정보를제공하고있으며이는품종개발등이용성이높을것으로판단된다. 또한 ICRISAT 에서개발한미니핵심집단의개념은작물육종프로그램에있어유전자원의이용을강화시키는목적으로연구자들에게인식되어지고있어제한된종자로구축한핵심집단및미니핵심집단이육종가들의연구와훈련에유용할것이라생각된다. * 교신저자 : Tel , hjbaek@korea.kr 46

103 PA-48 Sea-Hee Han, Kyung Jun Lee, Sebastin Raveendar, Gi-An Lee, Jung-Ro Lee, Myoung-Jae Shin, Gyu-Taek Cho, Kyung-Ho Ma * The National Agrobiodiversity Center (NAS, RDA, Republic of Korea) has been collecting the new valuable genetic resources continually. In this study, we collected 44 Vicia spp germplasm which includes wild type Vicia species in Korea. Genus Vicia is most popular as manure crops and cover crops in orchards. However, there are few studies on seed morphological characteristics. Therefore, we investigated the characteristics and diversity of seeds in order to provide basic information for its usage in breeding of agricultural traits. We investigated the morphological characteristics of Vicia seeds on the basis of 9 morphological traits (seed shape, seed color, seed surface, seed colour mottling, hilum shape, hilum colour, hilum length, seed length and seed width). Seeds size were spherical (45.5%) cubical (40.9%), and elliptical (13.6%). Seed color was black (47.7%), brown (22.7%), red-brown (11.4%), yellowish (15.9%), and Apricot (2.3%). Hilum shape was linear (79.5%), oblong (6.8%), and oval (13.6%). Principal component analysis (PCA) showed four principle components included PC-Ⅰ (30.7%), PC-Ⅱ (17.7%), PC-Ⅲ (14.1%), and PC-Ⅳ (11.3%). Cumulatively, the four components explained 73.8% of total variations. The cluster analysis based on morphological characters detected 5 main clades. This study provides the key insights to the characteristics of Vicia seeds and a basis information with Vicia spp. which could be useful for bio-industry. *Corresponding Author: Tel , khma@korea.kr PA-49 Phanna Phat 1, Kyung-Ho Ma 1, SeaHee Han 1, JaeJong Noh 2, Gi-An Lee 1, Kyu-Tae Cho 1, Kyung Jun Lee 1, and Ho-Jong Ju 3* Autotetraploids could be obtained spontaneously from the in vitro cultured cotyledon explants of small watermelons. Phenotypic dissimilarities observed in leaf size, reduction of fruit length and seed size and number of autotetraploid regenerants compared to its diploid donor plant suggesting a possibility of genetic alterations led to somaclonal variations or changes in genetic levels. Using AFLP and CAPS markers to analyze somaclonal variations between diploid donor plants and autotetraploids revealed a high polymorphism (64%, AFLP) and genetic alteration in gene control of fruit shape (CAPS). According to AFLP analysis, the highest number of fragments was found in diploid donor plants while all autotetraploid lines reduced approximately 6-27% of fragments in comparison to its diploid donor plant. The losses of fragments and genetic variations among diploid and autotetraploids clearly revealed genetic alterations during in vitro culture. UPGMA cluster analysis showed a significant separation between diploid and two groups of autotetraploids, indicating that AFLP is efficient for identifying the somaclonal variants. It may be the first report of using AFLPs and CAPS to identify somaclonal variations causing genetic changes in autotetraploids of small watermelon. These authors contributed equally to this work *Corresponding Author: Tel , juhojong@jbnu.ac.kr 47

104 PA-50 Sebastin Raveendar, Gi-An Lee, Kyung Jun Lee, Myoung-Jae Shin, Jung-Ro Lee, Gyu-Taek Cho, Kyung-Ho Ma * Little millet, Panicum sumatrense Roth ex Roem. & Schult., is an important cultivated species under the tribe Paniceae, sub-family Panicoideae and family Poaceae. In this study, we sequenced the complete chloroplast (cp) genome of P. sumatrense for the first time to investigate their phylogenetic relationship in the family Poaceae. The complete cp genome sequence of P. sumatrense is 139,384 bp in length with 38.6% overall GC content and exhibits a typical quadripartite structure comprising one pair of inverted repeats (22, 723 bp) separated by a small single-copy region (12,583 bp) and a large single-copy region (81,355). The P. sumatrense cp genome encodes 125 unique genes, which include 91 protein-coding genes, 4 rrna genes, 30 trna genes and 20 genes were duplicated in the inverted repeat region. This newly determined cp genome (P. sumatrense) could be valuable information for the breeding programs of this cereal crops in the family Poaceae. *Corresponding Author: Tel , khma@korea.kr PA-51 박명렬 1*, 서민정 1, 윤홍태 1 1 콩의기계화수확효율을증진시키기위해서는꼬투리와줄기, 잎이동시에성숙하는품종의개발이필요하다. 콩에서개화조절에관련된유용유전자에대한연구는다양하고심도있게진행되어졌지만성숙조절에관련된유전자의연구는미흡한편이다. 본연구는콩의꼬투리성숙관련유전체를분석하여성숙관련기능을갖는유전자를선발하고, 콩의부위별발현분석을통해평가된유전자를동시성숙성이높은콩품종개발을위한육종소재로활용하고자실시하였다. 발현분석용콩식물재료로는대풍콩을비롯하여성숙기가다른 18 품종을선발하였고, 각품종은꼬투리, 줄기, 잎으로분리하여유전자발현분석에이용하였다. 이미알려진 178 개꼬투리성숙관련유전자좌 (QTL) 영역내에 2,600 여종의유전자가존재함을확인하였고, 유전자들의 ontology 를분석하여 560 종의유전자가 seed development 등성숙관련기능을갖는것으로확인되었다. 유전자의발현분석을위해실시간유전자발현분석장치 (realtime PCR) 와성숙관련기능을갖는것으로확인된 560 종의유전자중염기서열정보가확인된 215 종유전자를이용하였다. 부위별로발현의양상이달라진유전자는 seed development 기능을갖는 Glyma.19G 를비롯하여 102 종이었고, 특히 senescence-associated 기능을갖는 Glyma.06G 등 21 종의유전자는만숙종인대풍콩보다 3 부위모두에서발현이 2 배이상증가하였다. Response to stress 기능을갖는 Glyma.19G 등 28 종의유전자는조숙종에서만숙종으로갈수록유전자의발현이증가하였고, oxidation-reduction process 기능을갖는 Glyma.18G 등 17 종은그반대의발현양상을보였다. 이런결과를통해 3 부위모두발현이 2 배이상증가하는유전자 Glyma.06G 등 21 종을동시성숙성이높은콩품종개발을위한육종소재로선발하였다. 사사 : 본연구는농촌진흥청아젠다사업 ( 과제번호 : PJ ) 의지원에의해수행되었다. * 교신저자 : Tel , park5260@korea.kr 48

105 PA-52 박은준 1, 양은영 2*, 문지혜 2, 이옥진 2, 채수영 2, 김상규 2, 김대현 2, 이금선 2, 김혜림 2, 박태성 3, 허온숙 3, 김옥례 4, 장길수 5, 박종숙 고추에서품종육성연한단축을위한반수체육종법중하나인약배양기술은국가연구기관및민간종자회사에서실제품종육성과정중중요한단계로자리매김하고있으며각연구기관및회사자체내에서고유프로토콜을보유하고있다. 최근에는약배양보다배가반수체획득효율이높은것으로알려진소포자배양기술에대한연구가진행되고있다. shed- 소포자배양기술은고체배지와액체배지를동시에이용하는변형된약배양으로고추에서다수의배를확보할수있는방법이다. 본연구에서는고추의 shed- 소포자배양기술확립을위해배지의 ph 및품종이소포자배발생에미치는영향을조사하였다. 밀양재래와고색소품종인 7QF4, 7QF9, 7QF36, 7QF38 등총 5 품종을재료로이용하였으며, 각품종에적합한 ph 를조사하였다. 밀양재래품종은전처리배지와배양배지모두 ph 6.0 일때소포자배발생효율이가장높았으며, 발생된배의총수또한가장많았다. 7QF4, 7QF9 품종의경우에는소포자배발생효율및배의총수모두전처리배지로 ph 8.0 을사용하고, 배양배지의 ph 를 4.0 으로조절하여사용한경우높았으나 7QF36, 7QF38 품종의경우에는각각다른경향을나타내었다. 5 가지품종을대상으로비교한결과최적 ph 가각기달랐으며, 소포자배발생효율및배발생총수모두품종에따른차이가크게나타났다. 고추 shed- 소포자배양시품종이소포자배발생및발달에미치는영향을조사하고자 12 개의품종을대상으로 shed- 소포자배양을실시한결과 1 개의꽃봉오리에서 1 개이상의배가발생한효율은 LV2319, ECO-1, 7QF4, 7QF9 순으로각각 95.8, 92.6, 60.8, 48.0% 로높았으며, 종간교잡 F 1 품종인 16NHC 품종의경우배가전혀발생하지않았다. 반면발생한배의총수는 LV2319, ECO-1, 밀양재래, 7QF4 순으로각각 24.8, 7.3, 6.3, 3.2 개였다. 본연구결과고정종인 LV2319 와밀양재래품종의경우배발생이높은것으로나타났으며, F 1 품종들에서는배발생효율이전반적으로낮게나타났다. 향후계속된연구를통해서형질고정정도및품종특성이소포자배발생에미치는영향에대한조사가필요할것으로판단된다. 본연구결과들은 shed- 소포자배양을통해반수체및배가반수체를생산하기위한연구의기초자료로이용될수있을것으로기대한다. * 교신저자 : Tel , yangyang2@korea.kr PA-53 박향미 1*, 이정희 2, 원용재 1, 정국현 1, 안억근 1, 현웅조 1, 정응기 우리쌀의도정수율향상을위하여쌀도정특성및입형을분석하고이와연관된분자마커를발굴하여분자육종에적용해보고자본연구를수행하였다. 연구결과를요약하면우선, 이삭특성과등숙율및도정수율과상관성을분석한결과, 등숙율은 2 차지경등숙율과정의상관의유의성 (0.764**) 인정되었고, 도정수율은주당수수와정의상관의유의성 (0.711**) 인정되었다. 따라서등숙율과도정수율을높이기위해서는 2 차지경등숙율이높고, 주당수수가많은것이좋은것으로나타났다. 다음으로, 도정특성및종자특성에대한 QTL 분석결과, LOD 값이 3.0 이상으로설명력이 10% 이상인형질은정현비율, 현미천립중, 길이, 너비, 현미장폭비이었다. 특히현미너비와장폭비, 현미천립중과관련된 QTL 은동일위치의 RM03328 마커로나타났으며, LOD 값이 15.0 과 13.0, 6.55 로설명력이 39% 와 35%, 20% 로높아실용적인 QTL 마커로활용성이예상된다. 또한, 정현비율과관련된 QTL 도 LOD 값이 4.54 로설명력이 14.1% 로나타나등숙률과도정수율이높은우량계통육성을위한분자육종에적용가능성을확인하였다. 이런결과들로볼때설명력높은 QTLs 연관분자마커활용으로등숙률과도정수율이향상된품종육성이가능할것으로기대된다. * 교신저자 : Tel , parkhm2002@korea.kr 49

106 PA-54 류시환 *, 박종열, 최재근, 남궁민, 최승출, 김문종, 용우식, 윤석원, 남경남, 박기진, 최준근 옥수수는세계에서가장중요한식량공급원중의하나이며, 유전육종적방법의발달과함께획기적인생산성증대의본보기이다. 단위면적당재식밀도의증가는옥수수생산량증대의또다른중요한요인이다. 미국 corn belt 지역의재식밀도와수량성변화를보면, 1930 년대에는 ha 당 3 만주파종하여 1.6 톤을수확하였으나, 현재는 ha 당 8 만주이상파종하여 9.5 톤을수확하고있다. 그러나국내에서는 ha 당 만주가일반옥수수표준재식밀도이다. 수량성증대를위해서는세계적인재배양식에따라밀식하는것이필요하다. 국내육성종실및사료용옥수수품종과밀식적응성이우수한수입품종을비교하여국내품종의밀식적응성을평가하고자본연구를수행하였다. 밀식재배시수입품종과국내육성품종의수량성을비교해본결과, 수입품종 (32W86) 의수량성을 100 으로할때, 국내품종의수량성은 의수준이었다. 시험한국내육종품종중에서밀식적응성이가장우수한교잡종은종교 128 호이며, 수량성은 32W86 대비 93 수준이었다. 밀식과표준재배에서국내육성품종의수량성및생육특성을비교해본결과, 밀식에서수량성이 28% 증수하여표준보다는밀식재배가수량증대효과가있는것으로확인되었다. 밀식재배의경우도복에약하였고, 간경, 이삭길이, 이삭폭및 100 립중은표준대비감소하였다. 국내육성품종은표준재식밀도에서선발되었으므로밀식적응성이수입품종에비해낮은것은당연한결과로판단된다. 향후세계수준의옥수수생산성을위하여국내에서도밀식재배에관심을가지고밀식적응성품종육성에노력해야할것으로생각되며, 밀식적응성품종육성은유전자원의개량뿐아니라도복등환경스트레스저항성선발이선행되어야할것으로판단된다. * 교신저자 : Tel , shr8921@korea.kr PA-55 Hwa-Yong Lee 1,2, Suyun Moon 1, Chang-Duck Koo 2, and Hojin Ryu 1* 1 2 Lentinula edodes is one of the most popular edible mushrooms in the world. Recently, the breeding and development of new cultivars of L. edodes have been actively facilitated, and thus the development of efficient molecular markers that can distinguish the new cultivars is required for protection of the breeder s rights. In this study, we developed cleaved amplified polymorphic sequence (CAPS) markers for the identification of L. edodes cultivars Sanmaru 1ho and Sanmaru 2ho. These markers were developed using whole genome sequence data from monokaryon strain B17 and resequencing data from other strains. A single nucleotide polymorphism (SNP) was identified on the position of scaffold 9 in Sanmaru 1ho and on the position of scaffold 2 in Sanmaru 2ho. DNA containing each SNP in each strain was amplified, and Sanmaru 1ho and Sanmaru 2ho was not cleaved by restriction enzyme TspRⅠ and HhalⅠ respectively. Therefore, Sanmaru 1ho and Sanmaru 2ho were distinguished from other cultivars. *Corresponding Author: hjryu96@chungbuk.ac.kr 50

107 PA-56 김춘송 *, 조영찬, 백만기, 박현수, 정종민, 남정권, 김보경, 이건미, 박슬기, 이창민 우리나라의밥쌀용벼품종은유전적배경이협소하므로식미를다양화하기위해고유유전자원인재래벼와잡초벼를활용한최고밥맛의고품질벼품종개발이시급하다. 유전적배경이다양한중생의고양식미품종개발을위해우리나라잡초벼와화영벼를교배하여육성한 F 5 세대 16 계통을생산력검정예비시험에공시하였다. 화영 / 횡성앵미 3 조합 7 계통과화영 / 나주앵미 15 조합 5 계통, 화영 / 남제주앵미 6 조합 4 계통에대하여주요농업적생육특성, 내병성, 미질및식미특성을대비품종인남평, 화영등과비교하였다. 공시한 16 계통중농업적특성이우수하고, 특히미질과밥맛및식미관련마커를통한기대식미치를종합적으로평가한결과화영 / 횡성앵미 3 조합 2 계통 (HR , HR ), 화영 / 나주앵미 15 조합 2 계통 (HR , HR ), 화영 / 남제주앵미 6 조합 2 계통 (HR , HR ) 등 6 개의우량계통을선발하였다. 선발한우량계통들은대부분출수기가 8 월 7 일부터 8 월 11 일인중생종에속하였으며수량성은화영에비하여 11 20% 높았으나남평과는유사하거나다소낮은특성을보였다. 내병성은도열병의경우중도저항성을보였고흰잎마름병 (K1 K3) 과줄무늬잎마름병에는강하였다. 쌀알은맑고투명하며백미완전미율과윤기치는대비품종과유사하였으나, 식미관련 13 개의마커를이용하여회귀식으로추정한기대식미치는 으로서대비품종인남평의 56.8 과화영의 74.3 보다높았다. 그리고식미패널에의한밥맛은남평 (0.00) 과화영 (0.14) 에비하여월등히좋은결과 ( ) 을보였다. 특히화영 / 횡성앵미 3 조합의 HR 과 HR 계통은기대식미치가각각 83.4, 93.3 으로높으면서밥맛도 0.45 와 0.52 로서가장우수한특성을보인유망계통이었다. 앞으로 유전자원접근 이익공유법 에따른자국생물자원에대한주권을강화하려는국제적추세에대응하기위해서는국내고유유전자원을이용한밥맛관련원천소재확보가매우중요하기때문에본연구에서선발한 6 개의우량계통을 2018 년생산력검정본시험에공시한후지역적응시험을거처고양식미품종으로개발할예정이다. * 교신저자 : Tel , kcs3925@korea.kr PA-57 Hyun-Su Park *, Man-Kee Baek, Jeong-Kwon Nam, Woon-Cheol Shin, Gun-Mi Lee, Seul-Gi Park, Chang-Min Lee, Choon-Song Kim, Young-Chan Cho To enhance rice yield and diversify grain quality of Korean japonica rice, we developed japonica rice line with long and spindle-shaped grain. Korean japonica rice cultivars have narrow genetic background of grain size and shape. Most of cultivars show medium-short and semi-round grain. To diversify the genetic background for grain, we developed Jeonju625, japonica rice line with long and spindle-shaped grain, derived from a cross between DSG79, the breeding material with extra-long and spindle-shaped grain, and Boramchan, japonica super high-yielding cultivar with medium-short and semi-round grain. Jeonju625 had GW2gs3qSW5+qGL3 allele type for grain-related genes, which conferred extra-long and spindle-shaped grain. The grain length and ratio of length to width of brown rice of Jeonju625 was 7.06 mm and 2.72, respectively. Jeonju625 was improved the deteriorated traits of DGS79, very late heading, long culm, long awn, droopy flag leaves, and susceptibility to lodging, by strong selection pressure focused on field breeding. The milled rice yield of Jeonju625 was 559 kg/10a, which was similar level of Boramchan (552 kg/10a) and 29% enhanced yield compared to DSG79. Jeonju625 had suitable characteristics for cooked rice. Jeongju625 showed lower protein contents and better glossiness and palatability of cooked rice than Boramchan and Hanareum2. Elite japonica rice line, Jeonju625, with extra-long and spindle-shaped grain showed good characteristics for rice yield and grain quality. Jeonju625 could be utilized practical cultivar and breeding material for enhancing rice yield and diversifying grain quality. *Corresponding Author: Tel , mayoe@korea.kr 51

108 PA-58 박현수 *, 백만기, 김춘송, 남정권, 이건미, 박슬기, 이창민, 서정필, 조영찬 우리나라자포니카벼의잠재수량성향상을위해서군집소수를가진고착립밀도이삭형벼를개발하였다. 교배모본으로 빈해수집 1 (IT251527) 과 ARC10319 (IT259789) 를이용하여단교잡인공교배하였다. 빈해수집 1 은강한도복저항성에이삭목이굵고이삭내착생립수가많아착립밀도가큰고착립밀도형이삭형태를가지고있으며, ARC10319 는키가크고이삭내지경의선단소수가 2-5 개씩군집되어있는군집소수형이삭형태를가지고있다. 빈해수집 1/ARC10319 와보람찬 /ARC10319 F 2 집단을이용한군집소수형이삭에대한유전분석결과군집소수형이삭은하나의우성유전자가불완전하게관여하는 1:2:1 의분리비를나타냈다. 분리세대에서군집소수고착립밀도이삭을가진개체를선발해나가면서고정계통을육성하였다. 예비선발시험에서 F 8 세대 23 개군집소수고착립밀도이삭형계통을선발하였고, 이들의이삭관련형질에대해서모부본및우리나라 178 품종 ( 자포니카 160 품종및통일형 18 품종 ) 과비교분석하였다. 군집소수고착립밀도이삭형계통들은주간의수당립수가 482 개 ( 개 ) 로빈해수집 1(342 개 ), ARC10319(302 개 ), 자포니카 (151 개, 개 ) 및통일형 (217 개, 개 ) 품종보다다립인이삭형태를나타냈다. 모부본에비해 1 차지경수와평균립수는비슷하거나중간값을가지나이삭목이두껍고 2 차지경수와평균립수가많아수당립수가증가하였다. 수당립수가증가한반면이삭길이는줄어들어착립밀도가증가하였고, 착생립의임실률은육성품종에비해크게떨어지는특성을나타냈다. 23 개계통중농업형질이양호하고수당립수가증가된 4 계통 (CD9, CD27, CD34, CD39) 을선발하여남평, 보람찬, 한아름 2 호, 모부본과함께생산력검정시험을수행하였다. 이들계통들의평균수당립수는 개로남평 (93 개 ), 보람찬 (96 개 ), 한아름 2 호 (114 개 ), 빈해수집 1(144 개 ), ARC10319(161 개 ) 에비해서증가하였으나수수가 9 개로적고 ( 남평 15 개, 보람찬 16 개, 한아름 2 호 16 개, 빈해수집 1 9 개, ARC 개 ) 등숙률이 %( 남평 89.1%, 보람찬 89.6%, 한아름 2 호 88.3%, 빈해수집 %, ARC %) 로낮아쌀수량성은 kg/10a 로모부본인빈해수집 1(429 kg/10a) 과 ARC10319(345 kg/10a) 에비해증가하였으나남평 (502 kg/10a), 보람찬 (554 kg/10a), 한아름 2 호 (766 kg/10a) 에비해적었다. 개발된군집소수고착립밀도이삭형계통은수당립수를증가시킴으로써잠재수량성을높인자원으로수당립수증대를위한육종소재로활용될수있을것으로생각된다. * 교신저자 : Tel , mayoe@korea.kr PA-59 박현수 1*, 남정권 1, 조영찬 1, 김보경 1, 백만기 1, 고재권 1, 김기영 2, 신운철 1, 김우재 1, 김정주 1, 이점호 1, 고종철 1, 정종민 1, 강경호 1, 정지웅 조농 은쌀의공급과잉에따른논이용다양화와사료의자급률을높이기위해개발되었다. 조농 은지금까지개발된사료용벼 8 품종중이삭패는데걸리는기간이가장짧은조생품종으로높은수량성을가지고있으며쓰러짐에강하여직파재배를이용한맥류의뒷그루재배가가능하다. 2010/2011 년동계에초기신장성이좋고도복에강한고바이오매스준조생유전자원 빈해수집 1(IT251527) 을모본으로하고자포니카초다수성품종으로소얼수중형초형에도복에강한중만생품종인 드래찬 을부본으로하여단교잡인공교배하였다. 고정계통을조기에확보하기위해서 2011 년하계에 F 1 식물체의이삭을채취하여약배양을수행하였다 년 210 개약배양계통중출수가빠르고바이오매스가큰계통을선발하여 년생산력검정시험을수행하였다. 공시계통중조생종이면서초기신장성이빠르며바이오매스가크고강한내도복성을가지고있는 HR29675-AC10 계통을선발하여 익산 581 호 로계통명을부여하였다. 익산 581 호 는 년 3 년간실시된사료용벼지역적응성검정시험결과출수기가평균 8 월 1 일로기존의사료용벼중가장빠른 녹양 의 8 월 12 일에비해 10 일이상빠르면서도조사료수량은 1,476kg/10a( 녹양대비 99%) 로비슷하고정조수량은 807kg/10a 로 녹양 보다 12% 가증수되어높은수량성을나타냈다. 또한가축이소화흡수할수있는사료의영양가지표를나타내는가소화양분총량 (TDN) 이 녹양 과같은 70.2% 로사료가치가우수하여 2016 년 12 월직무육성신품종선정위원회에서사료용신품종으로선정되었고조생종이면서조사료와농후사료에겸용으로활용될수있다는의미로 조농 으로품종명을명명하였다. 사료용벼 조농 은밥쌀용벼대체작물로논이용다양화와사료의자급률향상에기여할것으로기대된다. * 교신저자 : Tel , mayoe@korea.kr 52

109 PA-60 박슬기, 이건미, 이효정, 백만기, 박현수, 남정권, 김춘송, 김보경, 조영찬 * 쌀관세화에의한쌀시장개방으로수입쌀부정유통방지를위해국내육성벼품종과수입예상국가의쌀에대한분자표지를활용하여품종판별체계를확립하고자본연구를수행하였다. 공시재료는국내육성벼 320 품종과수입가능외국브랜드쌀 40 종, 일본주요자포니카 16 품종의시료를활용하였다. 식미관련마커, 도열병저항성연관마커와수입브랜드쌀의 re-sequencing 정보를활용하여개발한마커중 42 개를선발하여품종판별 DB 를구축할수있었다. 이들마커중재현성과다형성이높은 SNP_1T, SNP_5T, krif6, krif64, ImMF136, ImMF155, GPA 7 개의마커세트로국내벼재배면적을많이차지하는국내자포니카 34 품종과수입가능외국브랜드쌀 40 종, 일본자포니카 16 품종을분석한결과, 국내육성품종과외국품종을구분할수있었다. 그러나, 오륜벼등 6 개국내육성품종은외국품종과그룹화되었고, 수입브랜드쌀 B3 등 4 품종은국내육성품종과그룹화되었다. 국내육성품종과외국품종을명확하게구분할수있는최적의분자마커조합을개발하기위해서는추가적인마커선발이필요하다. * 교신저자 : Tel , yccho@hotmail.com PA-61 하건수 1*, 송윤호 1, 조영래 1, 오혜진 1, 조윤상 1, 이지우 1, 김상수 1 1 최근웰빙문화의확산으로건강과질병의예방및치료에대한소비자의관심이증가하면서건강기능성식품에대한수요도증가하고있다. 특히수수는곡류중탄닌을함유하는유일한작물로써다양한생리활성을갖는폴리페놀을함유하고있어건강기능성식품원료로써의이용가능성이매우크다. 그러나원료곡생산시생력재배를위한간장, 경태, 성숙기및콤바인수확수율등에서개선이필요한것또한사실이다. 따라서생력재배가가능하며소비자들의기호및수요에알맞은수수신품종 황금찰수수 2 호 를육성하였다. 육성모본으로는강원지역수수주산지의주재배품종인 황금찰수수 와울산지역수집종 (IT028592) 분리계통인강원 190 호를이용하였다. 육성목표는콤바인수확이가능한간장, 출수기등의생육특성과소비자선호건강기능성인항산화성분및항산화활성등이었으며, 육성과정중농업현장에서실증비교시험을수행하였다. 육성과정은 2004 년인공교배후 2010 년까지후대를양성하며선발과정을거쳤고 2011 년부터 2012 년까지생산력을검정하였다 년부터 2017 년까지강원지역춘천등 3 개소에서지역적응성을검정하였으며, 2017 년생력재배를위한농업현장비교시험을수행하였다. 황금찰수수 2 호 는다수성이며콤바인수확이가능한밀수형이삭으로찰성배유를가지며붉은색종피의혼반용품종이다. 가변특성으로간장 146cm, 이삭장 24cm, 천립중 26g 으로출수기가 8 월 5 일이다. 생력화를위한자탈형콤바인수확농가실증시험에서수량성은 390kg 으로파종부터수확까지생력화재배가가능하였다. 소비자가선호하는특성중품질특성은아밀로팩틴함량이 90.43% 로찰성배유이며, 무기성분증칼슘은 15.58mg/100g, 칼륨은 434mg/100g 이었다. 항산화성분에서폴리페놀은 mg/100g, 플라보노이드 mg/100g, 수용성탄닌 mg/100g 이었으며, 항산화활성은 DPPH 0.31±0.02, ABTS 2012±0.51 로매우높은기능성수수품종이다. * 교신저자 : Tel , redclover@korea.kr 53

110 PA-62 Wonwoo Cho 1*, Hyemin Lim 1, Jun-Won Kang 1, Hyunseok Lee 1, Sol-Ji Lee 1, Ganchudur Tsetsegmaa 2,3, Eunjeong Choi 2, Jiwon Han 2, Romika Chandra 2, Hoduck Kang The objective of this study was to select poplar clones with high adaptability and potential for advanced growth for combating desertification in Elsen tasarkhai an arid and semi-arid area in Mongolia. After the establishment of the research site in Elsen tasarkhai, the first screening was cunducted on 22 types of poplar clones. Based on the survival rate in the second year, DN 002, DN 247, DN 034, DN sim, TN 074, P. sibirica, P. simonii were selected for further studies. Studies on the morphological factors for clonal selection of the introduced poplar clones included measurement of survival rates, the relative growth rate of heights, collar diameter, characteristics of leaves, dry weights and differences of T/R ratio. As for physiological features, relative moisture content, water potential, photosynthesis and fluorescent reactions were measured. Biochemical analysis measurements included chlorophyll content, proline content, ascorbate and free sugar content. The measured values for adaptation index led to the decision on the adaptation capacity and the final selection of clones included P. sibirica, DN 034 and DN sim. DN 247 and TN 074 showed low adaptation capacity value. Selected poplar clone s adaptation index and the correlation analysis of morphological, biological and biochemical factor measurements revealed that the factors include survival rate, RWC, carotenoid, proline, total chlorophyll, glucose, fructose, and mannitol measured with positive correlations whereas the measured values with negative correlations were T/R ratio, Pn, Ci, Sucrose. *Corresponding Author: Tel , wonwoo.c@gmail.com PA-63 이지우 1*, 김성용 1, 정재환 1, 이혜리 1, 하건수 1, 송윤호 1, 조윤상 1 1 중생종대립향찰벼인 고향찰 은 2015 년강원도농업기술원에서육성한특수미품종으로누룽지향과찰벼특성을가지고있어떡, 혼반등쌀시장확대를통한수급안정에기여할것으로판단된다. 이에따라농가에안정적인종자보급을위한국가종자생산체계를구축하였으나재배확대에따른안정적인재배법확립이필요한실정이다. 따라서질소시비량차이에따른수량성을평가하고자 년에강원도농업기술원시험포장에서출수기, 수량구성요소및수량성등을조사하였다. 기타재배법은벼표준재배법을준용하였으며대조품종으로는 설향찰 을사용하였다. 질소시비량은 0, 7, 9, 11, 13 kg/10a 등 5 수준으로설정하였다. 시험결과, 생육은질소시비량이증가할수록엽면적지수및건물중이증가하였다. 수량구성요소인단위면적당수수는증가하였으나반면영화수는 N-9kg/10a 시험구에서가장많았고이후감소하는경향을보였다. 현미천립중은질소시비량이증가할수록무게가감소하는경향이었다. 백미수량은질소시비량이증가할수록증가하였으나 11kg/10a 이후증가폭이크게감소하여무비구를기준으로한수량지수에서 11kg/10a 시험구와 13kg/10a 시험구가같은수치를나타내었다. 따라서, 고향찰 의수량구성요소와수량을고려한 10a 당적정질소시비량은 11kg 내외로판단된다. * 교신저자 : Tel , paizem1987@korea.kr 54

111 PA-64 Sumin Jo 1, Tae-Heon Kim 1, Jun-Hyeon Cho 1, Ji-Yoon Lee 1, Yeong-Ho Kwon 1, Saes-Beul Lee 1, Joo-Won Kang 1, You-Chun Song 1, Dong-Soo Park 1, Jong-Min Ko 1, Jong-Hee Lee 1* 1 Japonica rice is getting popular and it has been able to attract huge number of consumers in Asian countries, particularly in the tropics. The temperate country-bred Japonica cultivars, such as the late-maturing varieties from Korea and Japan, show very low adaptability under tropical conditions as they are photoperiod- and thermosensitive varieties. Most of them are heading too early, the grain quality is also low, and yield is significantly reduced. So, there is urgent to develop temperate Japonica cultivars adapted to tropical conditions. Currently, the leading variety, IR142 (MS11), is lower in yield than the local Indica, and is also vulnerable to pests. Therefore, these traits need to be improved in order to expand seed distribution. YR29169 is a mid late maturing Japonica rice elite line adapted to tropical areas that was selected on the preliminary yield trial inirri. This elite lines was derived from three-way cross among Milyang265, Milyang244 and Suwon526. This elite lines have high-yield, which increased by 32% compared to IR142, and the number of grain per panicle increased by 38% and days to heading was longer by about 10 days. Grain yield exhibited significantly positive correlation with the number of grain per panicle (r=0.85 ** ), days to heading (r=0.89 ** ). Therefore, the results suggest that these traits can be used for selection of high yielding varieties in tropical areas. Also, this elite line has shown resistance against Brown planthopper (BPH), bacterial leaf blight (BLB) and Rice stripe virus(rsv). The protein content of this elite line is low. Eating quality analyzed by Toyo tastemeter was also higher than IR142. This result is indicating that this palatability of line is better than IR142 in taste. This results support that this elite line will be useful genetic resource developing for the adaptation of temperate japonica rice in the subtropical regions. *Corresponding Author: Tel , ccriljh@korea.kr PA-65 조광수 *, 조지홍, 박영은, 임주성, 장동칠, 최장규 감자는벼, 밀, 옥수수와함께세계 4 대식량작물로국내에서는매년약 20,000ha 가재배되고있다 년현재약 70 여품종이등록되었으나수미를포함한약 10 개의품종이전체재배면적의 90% 이상을차지하고있다. 국내에서는씨감자보급의민영화를통하여다양한민간업체, 지자체등에서다양한품종을생산하여보급하고있으나괴경형태, 화색등외부형태적특성으로는품종구분이매우어려운실정이다. 따라서본연구는국내에서주로재배되고있는수미, 대서, 대지등국내재배면적의약 95% 를차지하는 16 개의품종과 8,303 개의 SNP chip (Illumina SOLCAP Infinium 8 K chip) 을이용하여국내감자품종의유전적다양성을분석하고 16 개의감자품종을구분할수있는 5 개 SNP 마커를선발하였다. 전체 8,303 SNP 마커중 minor allele frequency (<5%) 를제외한결과약 4,000 개의 SNP 가선발되었다. 감자품종의평균 Observed Heterozygosity 가가장높은품종은 대서 로약 75% 였으며가장낮은품종은 홍영 으로 41% 로나타났으며평균적으로약 58% 로조사되었다. SNP 마커의평균 PIC (polymorphism information content) value 는약 로나타났다. STRUCTURE 를이용하여국내감자품종의집단구조를분석한결과 16 개의국내감자품종은 3 개의유전적조성 (delta K=3) 으로구분되었다. 16 개의품종을구분하기위하여 5 개의 SNP 마커를선발하여 TaqMan probe 를디자인하였다. 각각의 SNP 마커 allele 에 FAM 과 VIC 형광염색프로브를제작후 Real-Time PCR 을수행하였다. SNP 마커를 additive coding data set 형태로 allele discrimination 을수행한결과 16 개품종을효과적으로구분할수있었다. 또한시중에서유통되고있는감자칩으로부터 DNA 를추출하여가공품에사용된품종구분결과수미, 대서등이사용된것으로판별되었다. 사사 : 본연구는농촌진흥청아젠다사업 ( 과제번호 : PJ ) 의지원에의해수행되었음 * 교신저자 : Tel , kscholove@korea.kr 55

112 PA-66 Abinaya Manivannan, Jinhee Kim, Hye-Eun Lee, Eun-Su Lee, Do-Sun Kim and Seung-Kook Choi Recent advancements in next generation genome sequencing (NGS) platforms provide numerous opportunities for the development of molecular markers. Single-nucleotide polymorphisms are powerful tools for the genomics-assisted selection and breeding in horticultural crops. Radish (2n=2x=18) is an important root vegetable in Brassicaceae family which is highly cultivated and consumed in Asian countries. The sequencing of radish genome aids in the better understanding and tailoring of traits associated with economic importance. However, very little research has been progressed on the aspects of NGS assisted molecular marker development in Radish on comparison with Brassica rapa in Brassicaceae. In order to accelerate the genomics assisted breeding and genetic selection, transcriptomes of 35 radish inbred lines with diverse traits were sequenced for the development of SNP markers. The sequenced reads ranged from 90,560,400 to 38,048,086 with the GC (%) of to The phred quality score (Q30) of 97% were obtained in the present sequencing approach. The raw data were trimmed and utilized for the identification of SNPs using several bioinformatics tools. The results suggested the discovery of 64,746 SNPs which have been further filtered to 571 potential SNPs. Moreover the SNPs developed in the current endeavor will be genotyped and utilized for the genetic diversity analysis and molecular marker assisted breeding in radish. *Corresponding Author: Tel , Fax , PA-67 박영은 *, 조지홍, 이영규, 장동칠, 조광수, 임주성 우리나에서재배하는감자품종의약 60% 정도는미국에서도입하여국내적응성시험을통하여 1978 년에육성된 수미 (Superior) 품종이다. 이품종은더뎅이병에비교적강하지만감자잎말림바이러스 (PLRV), 감자역병 (phytoptora infestans) 등에약하여씨감자채종이어렵고산간지대여름재배작형에서방제비용이많이든다. 또한, 최근에기후온난화의영향으로기형서발생이증가하는등재배상많은문제점이발생하고있어이품종을보완할신품종보급이절실한실정이다 년육성된 다미 품종은숙기가중생이고지상부식물체는반직립성으로생장하여수광태세가좋아광합성에유리하다. 감자의모양은원형이며눈깊이가매우얕아조리에편리하다. 감자표피색은황색이지만고랭지재배시약한 Russet 형태를보이며육색은흰색이다. 잎은녹색이며꽃은흰색으로대체로개화량이많은편이다. 기형서, 열개서, 내부생리장해등생리장해발생은 수미 에비하여적다. 감자역병에대한포장저항성은 수미 에비해다소강한편이며, 진정저항성은중도저항성이다. 바이러스에대한저항성검정결과은 수미 는감수성, 다미 는중도저항성으로확인되었다. 평난지봄재배및고랭지여름재배시 수미 품종에비해수량이많고건물률은 20.5% 로비교적높다. 삶은감자는분질성으로맛이좋아일반부식용으로적당하다. * 교신저자 : Tel , papalove@korea.kr 56

113 PA-68 Hyejin An 1, Hwa-Yong Lee 2, Sebastin Raveendar 3, Tae-Young Heo 4, Yoon-Sup So 5 and Jong-Wook Chung 1* Button mushroom (Agaricus bisporus) is one of the most widely cultivated species in edible mushroom. Despite their economic importance, relatively little is known about genetic diversity of this strain due to their narrow genetic base. Illumina paired-end sequencing produced 43,871,558 clean reads and 69,174 contigs were generated from five offsprings. These contigs were subsequently assembled into 57,594 unigenes. These unigenes were annotated with reference genome in which, 6,559 unigenes were associated with Clusters of orthologous genes. GOntology classifications showed large numbers of unigenes were assigned. Based on genome data of five offsprings, 44 polymorphic SSR markers were developed. The major allele frequency ranged from 0.42 to 0.92; the number of genotypes and the number of alleles ranged from 1 to 4, and from 2 to 4; the observed heterozygosity and the expected heterozygosity ranged from 0.00 to 1.00, and from 0.15 to 0.64, respectively; and the polymorphic information content value ranged from 0.14 to The genetic distances and UPGMA clustering had showed discriminated offspring strains. The SSR markers developed in this study can be applied in the polymorphism analysis of button mushroom and cultivar discrimination. *Corresponding Author: Tel , jwchung73@korea.kr PA-69 안혜진, 권혁주, 정종욱 * 양송이버섯은전세계적으로가장많이재배되는식용버섯중하나로매년소비량과생산량이늘어나는추세이다. 그러나양송이의경제적중요성에비해분자유전학적연구는부족한실정이다. 나고야의정서발표에따라자국의유전자원에대한보호및주권강화에대한연구가필요하다. 본연구는양송이버섯의전장유전체를기반으로한국, 네덜란드등 6 개국가에서수집된 10 개유전자원을사용하여 SSR 마커를개발하였다. 213 개의 SSR 마커중 PCR 이되지않은 48 개 SSR 마커와단일대립유전자만나타나는 8 개의 SSR 마커를제외한 157 개 SSR 마커가다형성을나타냈다. 157 개 SSR 마커는유전적다양성을나타내는 PIC 값이 에서 로평균은 이었으며, 대립유전자의수는 2 개에서 9 개로평균 4 개였다. SSR 마커의 Repeated type 에따른분석결과 2bp repeated 에서 PIC 값과대립유전자의수가각각 과 3.4 개였으며 3bp repeated 에서는각각 과 3.7 개였다. 4bp repeated 에서는 PIC 값과대립유전자의수가각각 과 3 개였다. 개발된 SSR 마커는양송이버섯유전자원에대한품종구분및집단구조분석, 다양성분석등의연구에활용될수있을것이다. * 교신저자 : Tel , jakdukong@gmail.com 57

114 PA-70 방준형 1, 류호진 1, 정종욱 2* 1 2 감초는콩과의다년생식물로우리나라뿐만아니라세계적으로사용되는중요한약용식물이다. 현재감초는전세계적으로 20 종이분포하고있으며, 일반적으로만주감초 (Glycyrrhiza glabra), 유럽감초 (Glycyrrhiza uralensis), 창과감초 (Glycyrrhiza inflata) 의뿌리를통틀어감초라고한다. 감초의성분중 glycyrrhizin 은 triterpene 계열로주로항암, 해독, 항산화와같은약리작용을한다. 야생감초는재배종보다비생물학적, 생물학적요인들로부터강한저항성을가지고있으므로이와관련된유전자원을재배종에도입함으로써보다우수한자원을확보할수있을것이다. 본실험은감초야생종중의하나인 Glycyrrhiza lepidota 의전장유전체를기반으로 repeated type 2bp 인 AC, AG, GT 등 6 종류 60 개과 3bp 인 AAC, GTT, TTA 등 16 종류로 40 개, 총 100 개의 SSR 마커를제작하였다. 마커에대한다양성을나타내는 PIC 값은최대 0.730, 최소 0.346, 평균 이고, GD 값은최대 0.765, 최소 0.444, 평균 이다. 대립유전자의수는최대 5, 최소 2 이다. Repeated type 에따라 2bp 의 PIC 값은최대 0.730, 최소 이고 3bp 의 PIC 값은최대 0.685, 최소 이다. 본연구에서개발된마커는감초의유전적다형성, 집단구조분석품종구별등에활용될수있을것이다. * 교신저자 : Tel , jwchung73@chungbuk.ac.kr PA-71 Ji Seon Choi 1, Kyung Jun Lee 2, Sebastin Raveendar 2, Jinsu Gil 1, Yoon-Sup So 3 and Jong-Wook Chung 1* Licorice (Glycyrrhiza sp.) is an important medicinal herb, has long been used in traditional medicine for the treatment of several diseases worldwide. Understanding the genetic diversity within Glycyrrhiza species is important for the efficient conservation of these medicinal herbs. In this study, we have developed 20 polymorphic chloroplast microsatellite (cpssr) markers using the chloroplast genome of G. lepidota. The cpssr markers were tested on a total of 27 Glycyrrhiza individuals. The number of alleles per locus ranged from two to eight among the Glycyrrhiza accessions. Overall, the Shannon index (I) for each cpssr ranged from to 1.694, the diversity indices (h) were to and the unbiased diversity indices (uh) were to In addition, the cpssr markers were successfully divided the 27 Glycyrrhiza individuals into four groups. The cpssr markers developed in this study could be used in assessment of genetic diversity and rapid identification of Glycyrrhiza species. *Corresponding Author: Tel , jwchung73@korea.kr 58

115 PA-72 Ick Hyun Jo 1, Chi Eun Hong 1, Sebastin Raveendar 2, Kyong Hwan Bang 1 and Jong-Wook Chung 3* Licorice (Glycyrrhiza sp) is an important medicinal crop often used as health foods or medicine worldwide. The molecular genetics of licorice is under scarce owing to lack of molecular markers. Here, we have developed cleaved amplified polymorphic sequence (CAPS) and high-resolution melting (HRM) markers based on single nucleotide polymorphisms (SNP) by comparing the chloroplast genomes of two Glycyrrhiza species (G. glabra and G. lepidota). The CAPS and HRM markers were tested for diversity analysis with 24 Glycyrrhiza accessions. The restriction profiles generated with CAPS markers classified the accessions (2 4 genotypes) and melting curves (2 3) were obtained from the HRM markers. The number of alleles and major allele frequency were 2 6 and , respectively. The genetic distance and polymorphism information content values were and , respectively. The phylogenetic relationships among the 24 accessions were estimated using a dendrogram, which classified them into four clades. Except clade III, the remaining three clades included the same species, confirming interspecies genetic correlation. These 18 CAPS and HRM markers might be helpful for genetic diversity assessment and rapid identification of licorice species. *Corresponding Author: Tel , jwchung73@korea.kr PA-73 백정호 1, 이은경 1, 김년희 1, 김송림 1, 최인찬 1, 지현소 1, 최만수 2, 문중경 3, 김경환 1* 콩은식물성단백질및지방산의주요한공급원이며세계적으로중요한식량자원으로서종자의형태와색상은보관, 발아및기능과관련하여매우중요한의미를가진다. 식량과학원에서분양받은핵심집단의재배종 400 계통콩종자를 100 립씩상단 (Top) 1 장과측면 (Side) 4 장으로나누어촬영하여약 2000 장의이미지를획득하였다. 그리고, 분석프로그램 (ImageJ) 을활용하여각각의 39,065 립의이미지데이터를분리하였으며종자크기, 둘레, 부피등의정량적데이터를생산하였다. 이미지데이터에는유전적인돌연변이나환경적인요인에의해형태적으로특이한패턴을보이는개체들이확인되었다. 이러한개체들은계통내에서특정유전자의발현, 환경적응및피해에대한의미를갖기도하지만전체집단에서형질을분석할때왜곡된결과를도출하는요인으로작용한다. 따라서종자표면의매끄러움정도를 Convex 면적과 Area 면적의비율로판별하는 Solidity 값을이용해특이형태종자의검출방법을고안하였다. 하지만각계통마다다른형태적인특성으로 Solidity 값의기준이달라거리차에의한클러스터링방법 (Euclidean distance clustering) 을활용하여 400 계통에서 165 개전체기준으로약 0.4% 의특이형태콩종자를개별검출하였다. 이러한분석방법은병충해피해및환경적요인에의한특이한형태의종자를분류하는방법으로이용될수있고디지털표현형정보의품질을높여줌으로유전체정보를활용한유전자기능검정 (GWAS), 딥러닝을통한종자표현형연구등에정확하고신뢰성있는데이터를제공할수있을것으로예상된다. * 교신저자 : Tel , biopiakim@korea.kr 59

116 PA-74 Yang Jae Kang 1* 1 Machine learning applications, especially artificial intelligence (AI) prediction models impact the fields from science to industry. AI-based solutions on image recognition, natural language process, and sequential decision making problems were largely improved to have human like behavior that may replace human labors. In biology, researchers are trying to apply AI to solve biological problems, such as prediction of diseases, based on bio-bigdata massively generated by next generation sequencers and high-throughput imaging facility. However, another biological questions with regard to Proteins are hard to transit to machine learning problem because the protein sequence is not fixed in length to compare each other while the only way to compare has been sequence alignment among the proteins in same family. Here we benchmarked the natural language processing strategy for vectorization of sentences for our new strategy of changing protein sequences into fixed length of vector. The protein to vector strategy successfully represented protein identity similar with the sequence alignment strategy suggesting that this new strategy would accelerate machine learning based solutions on protein based questions such as protein-protein interaction prediction. Moreover, the precomputed protein matrix on well curated proteins from this strategy can be used for similarity search like BLAST without alignments and also without high computational burdens. *Corresponding Author: kangyangjae@gmail.com PA-75 Eun Ju Cheong 1*, Ik-Young Choi 2, Minho Na 1, Hwa Lee Prunus yedoensis is one of most popular ornamental tree species planting in roadside and parks in Korea. Also, there are many natural population in Jeju-Island that the wild morphological characteristics are not distinguishable to cultivate. We compared the chloroplast genome sequence and made up consensus cp genome sequence using the 6 individuals, in which two cultivates and 4 wild individuals are collected. The 4 complete cp genome sequence are generated by de novo assembly of the sequencing data generated by Illumina high-throughput sequencing platform and the other two cp genome sequence were obtained from Genebank. The consensus sequence was aligned as 158,149 bp with containing 86,210 bp of LSC, 19,153bp of SSC, and each 26,393 bp of IRA and IRB. A total AT ratio is 63.28% in consensus sequences. Although these are identified same species with the morphological characteristics, we found highly variable sequence in cp genome. A total of 168 SNPs and 531 INDELs were identified and these take 0.106% and 0.336% respectively. One individual was identified highly different cp genome from the others by neighbor-joining tree analysis. This would be good resource to study of genetic diversity in Prunus yedoensis. We identified SSRs from the cp genomes that discriminate the accessions and possibly use for the cultivar specific markers in the further investigation. Acknowledgement: This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2017R1D1A3B ). *Corresponding Author: Tel , ejcheong@kangwon.ac.kr 60

117 PA-76 Won-Tae Yang 1*, Ki-Deuk Bae 2 1 Dwarfing is one of the most important traits in rice, because regulatory mechanisms for plant growth and development. In rice, more than 60 dwarf mutants are well known and its present different phenotype, such as small grain, more tiller and leaf shapes or plant height. In last decade, manifold GA biosynthesis and signaling genes have been characterized in rice that are regulated cell division, development, hormones and various stress. In this study, a novel gene OsGASD (Oryza sativa Gibberellin Acid Sensitive Dwarf) was identified that an efficient system to create rice mutant by Ac/Ds transposon insertion mutagenesis, such as selected homozygous mutant in dwarf phenotypes. To research the function of dwarf OsGASD gene in GA biosynthesis pathway, we generated transgenic rice plants overexpressing OsGASD plants for analysis of GA signaling and uptake. Overexpressing OsGASD plants shown that internodes length, height, number of tillers and biomass were increased compared with wild-type plant, wherase osgasd mutant through Ac/Ds decreased. osgasd mutant includes smaller amount of active GAs than wild-type. osgasd mutant plant of GA biosynthesis pathway causes GA deficiency and dwarf plants, and endogenous GA suppliance can restore the wild type phenotype in this mutant. The result indicated that OsGASD gene regulated the elongation of shoot, stem, plant height and biomass. The increased expression of OsGASD gene dramatically induces expression of the factors associated with GA biosynthesis such as CPS, KO, KAO, GA20ox and GA2ox, whereas osgasd mutant suppression of the factors associated with GA biosynthesis, loading to dwarf phenotypes. That applied GA 3 at the plant development stage to survey the response of OsGASD gene to GA 3. We suggest that OsGASD gene is related to factors of GA biosynthesis pathway regulating rice biomass development. *Corresponding Author: Tel , wtyang@dau.ac.kr PA-77 김년희 1, 이홍석 1, 김송림 1, 이은경 1, 백정호 1, 지현소 1, 최인찬 1, 박부희 2, 안혜련 2, 김경환 1* 1 2 작물표현체연구는작물의형태적특징을영상데이터로수치화및객관화하여분석하는기술이다. 아시아열대지방등이원산지인호접란은화훼재배산업에서가장인기있는난초중의하나로교배종포함 20,000 여품종이넘으며최근소륜다화성미니호접란에대한관심이높아지고있다. 국립원예특작과학원에서호접란을분양받아 RGB phenome analyzer 를이용하여난초의형태적인특성을분석하여객관적인수치데이터로형질들을분류하고자하였다. 분석에사용된호접란 275 개체는꽃을 1 개체당최소 6 개씩카메라로촬영하여총 1,650 장의이미지를획득하였고, 영상처리프로그램 (Image J 와 Lemna Grid) 으로데이터를분석하였다. 분석된파라미터중국립종자원신품종심사를위한호접란특성조사표에따라꽃의크기, 너비, 길이의데이터를 5 단계로나누어정량화하였다. 너비 / 길이의비율 (Aspectratio), 이심률 (Eccentricity), 진원도 (Roundness) 와같은파라미터도새로운영상지표로호접란의형태적특성을분류할수있었다. 향후호접란의잎이나꽃대, 꽃차례등추가적인영상이미지를분석하고자하며기존의특성조사표를포함한새로운표현형특성지표를개발하여신품종육종과유전자기능분석, 정밀육종등에활용될수있을것으로기대된다. * 교신저자 : Tel , biopiakim@korea.kr 61

118 PA-78 최인찬, 김송림, 이홍석, 이은경, 김년희, 백정호, 지현소, 김경환 * 식물표현형연구는대량검정시스템 (High-throughput system) 을사용하여이미지데이터를획득하고분석하여식물의생장특성, 농업형질과의연관성을통해유전자기능분석, 육종선발등에활용한다. 영상을통한표현형분석과정의신뢰성을확인하기위해벼의초기생육과정에서실측값과이미지데이터와의상관관계를확인하고정성적인지표인색상변화를정량적으로표현하기위한연구를수행하였다. 동진벼 96 개체를파종후 16 일부터 65 일까지일주일단위로총 7 회벼의크기, 초장, 잎면적, 생체중과건물중을실측하고 RGB phenome analyzer 로식물체의이미지데이터를획득하였다. 실측과영상분석값의상관관계는 0.93 에서 0.99 로높아서, 초기생육시영상분석값이실측값을대체할수있을것으로판단되었다. 또한동진벼 40 개체를매달온실에파종하여 79 일까지재배하고 4 회반복하였으며매회 2 주부터일주일에한번씩총 9 회이미지데이터를획득하였다. 생성된영상데이터를이용하여생육특성과색상을분석하였으며색상은 HSI 색차계 Hue 값으로시작, 종료와단계값을지정하여빨간계열, 노란계열, 연한녹색계열과녹색계열로구분하였다. 보통작물재배기간이증가함에따라색차계의연녹색비율은감소하고녹색비율이증가하였으며파종후 65 일부터 72 일시기에녹색비율이감소하였다. 화분에질소비료를시비한후연녹색은감소하고녹색이증가함으로벼의색상변화가질소시비와연계됨을알수있었다. 향후이미지에서얻은식물의형태적, 색상적데이터를이용한정량적인식물표현형분석이가능하며유전자기능분석에도연계시킬수있을것이다. * 교신저자 : Tel , biopiakim@korea.kr PA-79 이은경 1, 백정호 1, 김송림 1, 김년희 1, 최인찬 1, 최만수 2, 문중경 3, 지현소 1, 김경환 1* 콩의종자는크기가작고둥근외형때문에버니어캘리퍼스같은장비를이용해서종자의장폭, 단폭등의표현형질측정시, 많은개체를일률적으로측정하기는어렵다. 최근주목받고있는영상을이용한표현체기술을이용하여콩종자의특성을분석하고자하였다. 국립식량과학원에서분양받은콩핵심집단 400 계통총 39,065 개체종자의영상데이터를획득후, 버니어캘리퍼스를이용한종자의실제측정값과영상을이용한측정값을비교분석했다. 상관계수는 0.95 이상으로높은상관관계를나타냈고, 영상분석을이용한표현형분석의신뢰도가높다는것을알수있었다. 종자크기측정시수분함량은평균 8% 였고, 수분함량에따라그크기및부피가변하기때문에수분함량에따른종자의표현형을분석하였다. 영상분석의신뢰성을높이기위해데이터품질개선과정 (Quality control) 을거쳐영상분석시결과의오차원인을제거하였다. 오차원인은주로충해, 종피균열, 외부압력에의한종자외형의변화또는혼입종자나영상촬영과정에서그림자에의한종자형태측정오차등이주된원인으로 1.96%(767 개 ) 를차지하였다. 데이터품질개선과정을통해서면적, 둘레, 부피등분석값의상관계수는전반적으로 0.95 이상이었으며둘레의상관계수는 0.86 에서 0.92 로최대폭으로증가하였다. 콩핵심집단 400 계통종자에대해영상을활용한표현형데이터를획득하였으며향후형태적특성분석, 표현형질과유전체정보와의연관분석 (GWAS) 을통해유전자기능을해석하는데활용하고자한다. * 교신저자 : Tel , biopiakim@korea.kr 62

119 PA-80 Jun Oh, Hyun-Ju Kang, Hyo Ja Oh, Song Lim Kim, Inchan Choi, Jeongho Baek, Kyung-Hwan Kim, Hyeonso Ji * Dense green leaf mutants can be utilized in discovery of genes regulating leaf growth and development. We found three dense green leaf mutant rice lines which are Ds061942, Ds073406, and Ds among the Ac/Ds insertion mutant population. For genetic analysis, the mutant lines were crossed to their wild type variety, Dongjin. All of F1 plants showed wild type phenotypes, and F2 segregation ratios were fit to 3:1 (wild type : mutant type), which indicated that single recessive gene controls dense green leaf trait in each mutant line. Resequencing of the three mutant lines were performed using Illumina Hiseq 2000 platform Gbp sequence data were produced per mutant line. After quality trimming and read-mapping onto rice reference genome sequence (Nipponbare), Gbp were mapped onto the reference sequence resulting in average mapping depth of x. By comparison with Dongjin resequencing data, 42,386-56,988 single nucleotide polymorphisms (SNPs) were found between each mutant line and Dongjin. MutMap analysis was performed by pooling mutant type F2 plants and resequencing the pooled DNA. It was found that the causative mutated genes were located on chromosome 3, 4, and 7. Map-based cloning of the genes regulating the dense green leaf trait of the mutant lines is under way. *Corresponding Author: Tel , jhs77@korea.kr PA-81 이홍석, 최인찬, 김년희, 김송림, 백정호, 김경환 * 관상용난초중호접란은한국, 일본, 유럽및미국에서가장인기가많으며, 2014 년에무역량이 2 억불이상의큰시장을형성하고있다. 이에국내에서도많은호접란의신품종을연구하고있다. 그러나국립종자원의분류방법인신품종특성조사요령은대부분육안에의한정성적분석으로이루어져있다. 이에영상을이용하여형태및색상을정량적수치로표기할수있는프로그램을개발하고이를이용하여객체분류및형태분류기술을사용할수있는지연구하였다. 호접란의꽃을꽃잎, 윗꽃받침, 측면꽃받침, 끝열편, 측열편, 캘러스 (Callus) 로총 6 개부분으로세분화하여자체개발한프로그램을이용하여분석하였다. 호접란의분석을위해서획득한영상은노이즈및배경제거, 색인화후형태학적수치와색상값을획득하였다. 영상데이터를통해획득된파라미터는기본특성을확인할수있는면적, 너비, 길이, 색상 (HSI-H*) 을객관적수치로서나타낼수있었다. 또한형태학적특징이잘나타나는이심률 (Eccentricity), 진원도 (Roundness), 컨백스홀영역 (Convex hull area), 밀집도 (Density) 를정량적수치로분석할수있었다. 개발된형태학적특징중에이심률과진원도를통해꽃잎및꽃의기관의원형도와표면의굴곡을확인할수있었다. 본연구를통해기존의특성조사표와개발된분석방법을통해특성분류에사용된다면더욱정밀한분류를실행할수있을것이다. * 교신저자 : Tel , biopiakim@korea.kr 63

120 PA-82 정지희 1*, 류민하 1, 권용락 1, 송현진 1, 김호방 헛개나무 (Hovenia dulcis) 는갈매나무과헛개나무속에속하는낙엽교목으로우리나라에는헛개나무속에한종만자생한다. 헛개나무과병에서추출한활성화합물이숙취해소간경화방지등에효과가있다는연구결과가알려지면서한약재뿐아니라건강음료등으로널리활용되어그수요가증가하고있다. 이에따라헛개나무자생지는급격히훼손되고있는데반해관련유전육종연구및보존관련연구는거의전무한실정이다. 본연구에서는헛개나무에대해다양한유전연구에활용가능한 microsatellite 표지를개발하였다. Glenn 과 Schable (2005) 방법에따라 microsatellite enrichment 라이브러리를작성하였다. 이를기반으로 microsatellite repeat 을가지는 197 개 contig 서열 (Genebank Acc. KP KP246700) 을확보하고모든 contig 서열정보를이용하여후보프라이머를제작하였다. 6 개헛개나무샘플을이용하여증폭양상을확인하여최종적으로변이가있고재현성이있는 21 개의프라이머를선발하였다. 충주 (34), 제주 (34), 강릉 (28) 세집단으로부터수집된 96 개헛개나무샘플을이용하여프라이머별특성및집단분석을수행하였다. 최종선발된 21 개프라이머의특성을확인한결과각프라이머에서는최소 2 개에서 14 개의대립유전자 (A, 평균 5 개 ) 가확인되었다. 이형접합도관측치 (H o ) 와기대치 (H e ) 는각각 ( 평균 0.488) 과 ( 평균 0.520) 의범위에서확인되었다. 14 개유전자좌에서 3 개집단의유전다양성을분석한결과대립유전자수 (A) 는집단에따라 , 이형접합도기대치 (H e) 는 로집단에따라큰차이가없었다. 집단내근친교배정도를예측할수있는고정계수 (F) 는 에서 으로 0 에가까운값을보여거의평형상태에가까운것으로확인되었다. 본연구를통해개발된 microsatellite 표지는헛개나무의유전다양성평가와육종연구에유용할것으로기대된다. * 교신저자 : Tel , florajh@korea.kr PA-83 박태일 1*, 박종호 1, 김양길 1, 박형호 1, 한옥규 1, 우제훈 2, 홍기흥 3, 최규환 4, 강천식 1, 윤영미 1, 손재한 1, 최창현 1, 이점호 귀리는다른맥류보다단백질함량과가소화양분함량 (TDN) 이높아사료가치가우수하고가축의기호성도뛰어나지만가을에파종하여재배할때추위에약한단점이있다. 따라서추위에강하면서건물수량이많고사료가치를높여수입종자를대체할수있는겉귀리품종개발이요구되었다. 이에국립식량과학원에서는추위에강하면서, 엽신비율이높고, 건물수량이많으면서도사일리지품질이좋은조사료용겉귀리신품종 신한 을육성하였다. 신한 의 3 년간지역적응시험결과, 이삭패는시기가평균 5 월 6 일로 삼한 보다 1 일정도늦었으나, 초장은 113cm 로 삼한 보다약간크며, 엽신비율이 16.0% 로 삼한 보다높다. 추위견딜성은 삼한 과대등하며, 경기도연천의낮은이랑재배에서내한성검정결과 100% 생존하였다. 신한 은현재보급되고있는 삼한 에비해생체수량과건물수량이헥타당각각 40 톤, 15.3 톤으로 6%, 8% 높다. 조단백질함량은 6.3%, 가소화양분함량 (TDN) 이 62.1% 로사일리지품질도 1 등급으로우수하다. 신한 은 1 월평균최저기온이 6 이상이고중산간지가아닌지역에서재배가가능하며, 조사료생산경종및축산농가의확대재배가기대된다. * 교신저자 : Tel , parktl@korea.kr 64

121 PA-84 최현모, 박단비, 배은경, 최영임 * Basic helix-loop-helix (bhlh) 도메인을갖는전사조절유전자는식물의생장과발달에중요한역할을하는것으로알려져있다. 본연구에서는발달과관련된유전자중에서 bhlh 도메인을갖는유전자를현사시나무에서분리한다음형질전환현사시나무를제작하였다. 이유전자의발현이증가된형질전환현사시나무는수고와직경생장이대조구에비해약 30% 이상증가하였다. 광학현미경을이용하여줄기의조직학적특성을분석한결과이차목부의세포수및크기가증가한것으로나타났다. 그리고식물의생장과관련된브라시노스테로이드생합성관련유전자들의발현에차이가나타났다. 형질전환현사시나무에서직경생장과발달에관여하는유전자들의발현변화를분석하기위하여마이크로어레이분석을실시하였다. 그결과 2 배이상발현이변화하는 1,276 개유전자를선발하여네트워크분석을통해 381 개유전자의상호관련성을확인하였다. 이를통해 MYB, NAC, WRKY, WOX 등생장과발달에관여하는전사인자들이 bhlh 유전자에의해조절될수있는표적유전자로추정되었다. 이들유전자의프로모터서열을분석한결과, bhlh 의결합부위로알려진 G-box 및 E-box 서열을포함하고있었다. 그리고선발된표적유전자들을 qrt-pcr 로검증한결과 bhlh 의발현이증가된형질전환현사시나무에서이유전자들의발현이증가하였다. * 교신저자 : Tel , yichoi99@korea.kr PA-85 최지원, 최현모, 최영임, 이효신, 배은경 * 현사시나무에서건조처리에의해발현이증가하는 stress-associated protein 11(SAP11) 유전자를분리하였다. 이유전자의발현을증가또는억제시킨형질전환현사시나무를만든다음환경스트레스에대한저항성을조사하였다. 그결과 SAP11 발현억제형질전환현사시나무는대조구에비해높은내염성과내건성을나타내었다. AP11 발현억제형질전환현사시나무의생장특성을확인하기위하여야외포지에식재하였다. AP11 발현억제형질전환현사시나무는대조구에비하여수고생장이저해된반면줄기생장과가지의수는증가하였다. 식재후 2 년간의모니터링결과뿌리를제외한상층부의생중량이 1.5 배그리고가지수는대조구에비해 2 배이상증가하였다. 가지발달에관여하는것으로알려진신호전달계유전자들의발현을조사한결과옥신관련유전자들의발현은감소한반면시토키닌관련유전자들의발현은증가하였다. 또한, 가지분화를억제하는것으로알려진스트리고락톤관련유전자들의발현이감소하였다. 따라서 AP11 발현억제형질전환현사시나무는건조지및간척지와같은척박지에서목질계바이오매스생산에유용하게활용될수있을것으로판단된다. * 교신저자 : Tel , baeek@korea.kr 65

122 PA-86 Suk-Bok Pae 1*, Myung-Hee Lee 1, Eun-Young Oh 1, Sung-Up Kim 1, Jung-In Kim 1, Un-Sang Yeo 2, Ki-Won Oh 3, Do-Yeon Kwak 1, Yong-Chul Kim Korean usually like to consume large grain of peanut for the roasted or boiled. One of peanut breeding programs is also focused on developing early maturing and high yielding vegetable peanut eaten after boil. A new peanut variety "Sewon"(Arachis hypogaea ssp. fastigiata.) with large grain and high yield was developed in the Department of Southern Area Crop Science, NICS, in Milyang This variety was developed from the crossing line between variety "Charmwon" with red testa Shinpung plant type and variety "Pungsan"(Milyang 27) with large grain Virginia plant type. "Sewon" which is short stem and semi-erect Shinpung plant type has 35cm of main stem length, 51cm of branch length and 13 branch number per plant. Each pod has two grains with ellipse shape of red testa and its yield components are composed of 38 mature pods of per plant, 113g of 100-seed weight, 72% of pod shelling ratio, and 81% of mature pod ratio in the regional yield trials(ryt). Its seed quality show 31.7% of crude protein and 35.2% of crude oil and 52.3% of oleate in fatty acid composition. This also have moderate resistance to early and late leaf spot, and more resistant to stem rot and lodging, compared with check variety Daekwang. Owing to these superior growth characteristics of more lodging resistance and source ability in late maturing stage, the average yield of "Sewon" was more productive than reference variety by 21% with MT/ha for fresh pod and by 16% with 5.23 MT/ha for dried grain in 3 year regional yield trials. *Corresponding Author: Tel , paesb@korea.kr PA-87 Sovetgul Asekova 1*, Sung Up Kim 1, Eun Young Oh 1, Krishnanand P. Kulkarni 2, Myoung Hee Lee 1, Jung In Kim 1, Suk Bok Pae 1, Do Yeon Kwak 1 and Tae Joung Ha Sesame is an important oil crop widely grown in Asian countries, including Korea. The genetic relationship and population structure analysis help to infer the history, gene flow, and domestication time of the crop and its wild ancestors, which is critical for designing breeding methods and for the development of improved cultivars. In the present study, we report the successful application of genotyping-by-sequencing (GBS) in revealing the genetic relationship and diversity in 89 black and white seed coat sesame genotypes, mostly from Korea. The GBS libraries were constructed using restriction enzyme ApeK1. More than a half million (575,712) SNPs were identified from the GBS data and filtered with the criteria of minor allele frequency (MAF)> 5%, missing data <30%, and with less than 3 read depth. The genotypes containing more than 30% missing genotype data were also removed. The final dataset comprising 9041 high-quality SNPs were then used for genetic diversity analysis. The STRUCTURE analysis divided the sesame genotypes into five subgroups, whereas the distance-based phylogenetic analysis formed 8 subgroups. The black-seeded wild sesame accessions and the gray-seeded Indian accessions formed distinct clusters. Similarly, the genotypes with white seed coat were clustered together, whereas, the light brown-, yellow- and brown-seeded genotypes clustered together at the close proximity or admixed with the white- or black-seeded sesame genotypes. The genotypes particularly from Korea were found to cluster together in each of the classified groups and were clearly distinct at both genetic as well as phenotypic levels. These results would be useful in understanding the genetic structure of the rough (black) and smooth seeded (white) sesame genotypes from Korea. *Corresponding Author: Tel , asekovasovetgul@korea.kr 66

123 PA-88 Sovetgul Asekova *, Sungup Kim, Eunyoung Oh, Myoung Hee Lee, JungIn Kim, Suk-Bok Pae, and Tae Joung Ha Recent genetic diversity studies involving sesame genotypes from Korea indicated the narrow genetic pool of sesame cultivars or inbred lines. The genetic diversity analyses utilizing molecular markers provides means to select diverse germplasm for variety development, thereby improving the genetic base. Additionally, such studies help to select the genotypes with a diverse background, which also might show variation for different phenotypes such as biotic or abiotic resistance. In the present study, we determined the genetic diversity and phylogenetic relationship among 86 Korean sesame genotypes, which included 68 (to 2 pathotypes of Phytophthora nicotiane; KX160004, KX160000) -susceptible and 18 ( KX160004, KX160000) -resistant genotypes. Over nine thousand genome-wide SNP markers were used to screen the selected set of phytophthora susceptible, and resistant genotypes. A dendrogram based on UPGMA tree showed that all the sesame genotypes could be grouped into distinct clusters. Ten of the 18 resistant genotypes were clustered into the groups having the majority of the resistant genotypes. The remaining 8 resistant genotypes, however, were found to be dispersed across the dendrogram tree and grouped with the susceptible genotypes. The results showed that the eighteen sesame phytophthora resistance genotypes have the distinct genetic background. Such diverse parents will be used for the development of mapping populations for Phytophthora resistance genes from different phylogenetic groups and for wise utilization of the Phytophthora-resistant germplasm in sesame breeding programs. *Corresponding Author: Tel , asekovasovetgul@korea.kr PA-89 Jongwook Ahn 1, Seula Choi 1, Min-Young Kang 1, Joung-Ho Lee 1, Jin-Kyung Kwon 1, and Byoung-Cheorl Kang 1* 1 Yellow leaf curl symptoms are caused by TYLCV (Tomato yellow leaf curl Kanchanaburi virus) and PYLCV (Pepper yellow leaf curl Thailand virus) viruses belonging to the Begomovirus group in the Geminiviridae family. Begomoviruses, circular single-stranded DNA viruses cause severe crop losses in tropical countries, particularly in tomato and pepper. In this study, TYLCKaV and PYLCThV were isolated from the yellow leaf curl symptomatic leaves of pepper. The viral genome was sequenced and infectious TYLCKaV and PYLCThV clones were developed. Both DNA-A and DNA-B were designed to have partial tandem repeats and were inserted into the pich86988 binary vector using golden gate and restriction enzyme cloning method. Agrobacteria containing each constructed clone were infiltrated into tobacco, tomato, and pepper to test their infectivity. One hundred percent infectivity was observed in the tobacco plants showing typical yellow leaf curl symptoms. Among two infectious clones, only TYLCKaV exhibited viral symptoms in tomato. However, TYLCKaV did not show any viral symptoms and no virus accumulation was observed in pepper. Now, we are testing the infectivity of PYLCThV infectious clones in pepper. *Corresponding Author: Tel. 02) , bk54@snu.ac.kr 67

124 PA-90 Saima Akhter, Elnura Torutaeva, Sung Aeong Oh, Soon Ki Park * Pollen development, a critical step for successful plant proliferation, is proceeded by an asymmetric pollen mitosis I and a symmetric pollen mitosis II under the elaborate genetic control. In order to identify genes important for the pollen development we morphologically screened DAPI-stained mature pollen grains from an activation tagging pool. As a result we isolated a heterozygous mutant line, AP-29-38, producing high levels of abnormal pollen grains. Detailed phenotypic analyses showed that the mutant microspores divide abnormally at pollen mitosis I. In addition, reciprocal crossing results revealed that genetic transmission of the mutant allele is highly reduced both through the male and the female, suggesting the gene function of the responsible gene is important for both sexes. Since the mutant line contains multiple T-DNA insertions we performed a map-based cloning approach and narrowed down to a handful of candidate genes including At1g50710 as the strongest candidate. At1g50710 encodes AUGMIN4, a member of the augmin complex which consists of eight subunits and plays an essential role for dynamic microtubule organization. Currently, we are carrying out genetic complementation analyses. Moreover, to confirm that AUG4 gene is responsible for the mutant phenotype SALK line independent T-DNA insertion lines analysis is in progress. *Corresponding Author: Tel , psk@knu.ac.kr PA-91 최장규 1*, 조지홍 1, 박영은 1, 임주성 1, 조광수 1, 장동칠 1, 천충기 1 1 우리나라감자품종육성은주로교배육종을통해서육성되고있으며실생세대부터 1 년에 1 세대가진전되어신품종출원까지 9 10 년이소요되고있다. 국내가공용감자시장은연간 20% 이상성장하고있어새로운감자품종에대한요구가증대하고있지만기존의육종체계에서는장시간이소요된다. 감자육종체계에서세대와연한을단축하여조기선발할수있는기술과체계를개발할필요가있다. 본연구는여름에진정종자를파종하여 9 월에선발하고이듬해여름에다시실생 2 세대를진전시키는기존육종방법을대신하여겨울온실에서진정종자를파종하여선발하였다. 그리고그해가을과이듬해봄에세대를진전시켜세대와연한을단축하고실생초기세대에서포트크기에따른생육, 괴경특성을비교하고자한다 년겨울온실에 G15D01 조합등 5 개교배조합의실생 1 세대포트별생육특성은대포트에서경장이 cm 로소포트 cm 보다경장이더길었다. 포트별괴경특성조사에서는대포트에서주당괴경수, 괴경중이 개 / 주, g/ 주로더많았다. 5 개교배조합의실생 2 세대선발은열개서, 기형서등생리장해가 G15M02, D03 교배조합계통에서 46 %, 40 % 발생하여도태되었다. 포트크기에따른괴경균일성은교배조합별로달관지수가 로포트의크기에상관없이괴경크기가균일하였다. 수량성에서는조합별로는 G15D01 교배조합에서달관지수가 로제일낮았으며포트크기에따른수량성은소포트에비해대포트에서선발한계통에서수량성이높았지만 G15D03 교배조합에서는소포트에서선발한계통에서수량성이더높았다. 실생 3 세대에서봄재배적응성검정과괴경, 가공성특성평가를통해실생초기세대육성방법에따른선발효율을분석하고감자육종연한단축과조기품종화가기대된다. * 교신저자 : Tel , dndn012@korea.kr 68

125 PA-92 Saminathan Subburaj 1, Luhua Tu 1, Ka-Youn Lee 1, Yong-Sam Jeon 1, Sangsu Bae 2, Geung-Joo Lee 1* 1 2 RNA-guided genome editing using the CRISPR/Cas9 CRISPR (clustered regularly interspaced short palindromic repeats)/cas9 (CRISPR-associated protein 9) system has been reported to have target-specific modifying potential in many crops. Plant protoplasts are useful for assessing the efficiency of CRISPR/Cas9-mediated mutagenesis. Here, we report successful targeted mutagenesis of a floral pigmentation gene called flavanone 3-hydroxylase (F3H) in Petunia protoplast cells. We transiently introduced RNA-guided endonuclease (RGEN) ribonucleoproteins (RNPs) complex with different sgrnas targeting five F3H regions in to isolated protoplast cells of Petunia (cv. midnight ). High resolution melt (HRM) curve analysis showed that the F3H gene was mutated at the targeted sites in the protoplast transfectants. In addition, the mutagenesis of INDEL (deletions and insertion types) at the targeted loci was confirmed by targeted deep DNA sequencing. Mutation rates from those five F3H-RGEN target sites was estimated which ranging from 0.8% to 49.3% with an average of 20.8 ± 7.2 %. A further analysis showed that the average ratio of deletion to insertion produced by the five F3H-targeting RNPs (F3H1 5) was about 45.4:54.6. Our results demonstrated that direct delivery of the RNPs into protoplast cells of Petunia can be exploited as an efficient tool for genetic manipulation of flower colors in important ornamental flowering plants without the use of traditional transgenic approaches. Keywords: F3H gene, flower color, RNA-guided endonuclease, protoplast, HRM assay, deep sequencing *Author for correspondence: Prof. Dr. Geoung-Joo Lee, gjlee@cnu.ac.kr PA-93 Jayern Lee 1, Sangrea Shim 1, Jungmin Ha 1,2, Suk-Ha Lee 1,2* 1 2 Vigna is a genus of flowering plants in the legume family, and about 100 species belong to this genus. Azuki bean, blackgram and mung bean are the representative crops of the species which are cultivated and consumed as human food in Asian country-wide. Unlike other major crops, phylogeny of Vigna species has not been classified using chloroplast genome, although the method has been established and generalized in other plant species. With the advancement of sequencing technology, RNA-seq became one of the most powerful tools for analyzing biological characteristics and numerous data have been produced for various purposes. In this study, phylogenetic trees of seven Vigna species were constructed using DNA and RNA sequences and compared. Each data was aligned to chloroplast reference genome of Vigna radiata to analyze variant among the species, using BWA for DNA and Tophat for RNA under consideration of sequence characteristics. As results, 1,836 nucleotide variations were identified using DNA sequence and distributed mainly along small single copy and long single copy region of chloroplast genome of V. radiata. While, only 165 variants were identified when using RNA-seq and distributed only on rrn23, psbc and psbd. Despite the relatively low number of sequence variations in RNA-seq compared to the result from DNA sequence data, the phylogenetic trees constructed using DNA and RNA sequence were consistent to each other, represent divergence of Vigna species. Consequently, we constructed phylogenetic tree of 22 Vigna species using 59 variants derived from RNA-seq data. In conclusion, phylogenetic analysis using RNA-seq is practically applicable to evolutionary studies in Vigna species. Furthermore, based on this phylogenetic study, gene expression patterns of chloroplast is under analysis. *Corresponding Author: Suk-Ha Lee, Tel , sukhalee@snu.ac.kr 69

126 PA-94 Trang Thi Do 1, Na-Hyun Shin 1,2, Maricar Ferrer Flores 1, Soo-Cheul Yoo Chlorophyll breakdown is a vital catabolic process of leaf senescence and fruit ripening as it allows for recycling of nitrogen and other nutrients. In this study, we found out that T-DNA insertional line ck40 maintained greenness with increased chlorophyll retention during senescence, while photosynthetic competence was not normally maintained during dark-induced leaf senescence, indicating ck40 is a non-functional stay-green mutant. qrt-pcr analysis of the genes related to chlorophyll catabolic pathway showed that transcript level of NOL was significantly lower especially in the early stage of senescence. This indicates that CK40, a receptor kinase, may indirectly control NOL expression by regulating a transcription factor. Ultrastructural analysis revealed that thylakoid membranes were thick but reduced in size in the chloroplasts of senesced ck40 mutant. In addition, chlorophyll b was highly accumulated as well as Light-harvesting chlorophyll b-binding proteins and a core protein PsbA remained stable in ck40 mutant than the wild type after dark-incubation. By hormone treatment test, we further revealed that CK40 function is involved in signaling of MeJA and ethylene-induced senescence but not in ABA. Agronomical traits analysis revealed that loss of CK40 function showed significant decreases in spikelet number and length of panicles, leading to yield reduction. Conclusively, these results indicate that CK40 function is critical for the regulations of spikelet number and yield as well as degradation of chlorophyll b and photosystem II during dark-induced senescence. Further analysis is necessary to identify the target of CK40, possibly a transcription factor, in order to elucidate CK40-mediated senescence mechanism. *Corresponding Author: Tel , dothitrang @gmail.com PA-95 Soo-In Sohn 1*, Young-Ju Oh 2, Ancheol Chang 1, Sun-Woo Oh 1, Gang-Seob Lee 1, Do Won Yun 1, Sung-Dug Oh A number of studies have been conducted on hybridization between transgenic Brassica napus and B.rapa or backcross of F1 hybrid to their parents. However, trait changes must be analyzed to evaluate hybrid sustainability in nature. In the present study, B.rapa and transgenic(bragl20) B.napus were hybridized to verify the early flowering phenomenon of F1 hybrids, and F1 hybrid traits were analyzed to predict their impact on sustainability. Flowering of F1 hybrid has been induced slightly later than that of the transgenic B.napus, but flowering was available in the greenhouse without low temperature treatment to young plant, similar to the transgenic B.napus. It is because the BrAGL20 gene has been transferred from transgenic B.napus to F1 hybrid. The size of F1 hybrid seeds was intermediate between those of B.rapa and transgenic B.napus, and ~40% of F1 pollen exhibited abnormal size and morphology. The form of the F1 stomata was also intermediate between that of B.rapa and transgenic B.napus, and the number of stomata was close to the parental mean. Among various fatty acids, the content of erucic acid exhibited the greatest change, owing to the polymorphism of parental FATTY ACID ELONGASE1 alleles. Furthermore, F2 hybrids could not be obtained. However, BC1 progeny were obtained by hand pollination of B.rapa with F1 hybrid pollen, withanout crossing rate of 50%. *Corresponding Author: Tel , sisohn@korea.kr 70

127 PA-96 Yedomon Ange Bovys Zoclanclounon 1, Hong-Il Ahn 1, Ji-Nam Kang 2, Ung-Han Yoon 1, Tae-Ho Kim 1, Byoung-Ohg Ahn 1,2, Keunpyo Lee 1* 1 2 Sesame is an important oilseed crop throughout the world. Phenotypic and genetic traits are crucial elements for crop improvement regarding yield and relative desirable traits. The aim of this study is to investigate phenotypic and genetic diversity of a worldwide collection of sesame. A total of 445 accessions from Africa, Asia, America and Europe continents were tested under field conditions for agronomic traits. The field experiment was designed following the augmented block design. The check genotypes were eight-time replicated. The space between two plants as 0.2 m and the sowing line per accession was 1.4 m. Agro-morphological parameters of early stage were recorded during the plants development. DNA extraction of each genotype will be performed and SSR molecular markers will be used for genetic analysis study. The phenotypic and genetic patterns of sesame accessions will provide preliminary information relative to the best accessions based on their performances. A second year of experiment will be planned following by genome comparison of the selected genotypes for a genomic variation investigation. *Corresponding Author: Tel , kplee@korea.kr PA-97 Hong-Il Choi *, Sung-Il Lee, Sung Min Han, Jae Wan Park, Sang Hoon Kim, Yeong Deuk Jo, Min Jeong Hong, Jin-Baek Kim, Si-Yong Kang In mutation breeding, ionizing radiations are widely used as physical mutagens. Proton beams (PBs) are known to have physically intermediate properties between low linear energy transfer (LET) gamma rays (GRs) and high LET ion beams, but their mutational effects have still not been characterized. This study was conducted to estimate the biological effect of proton beam irradiation compared with gamma irradiation and investigate the optimal dose for mutation induction in rice. Rice seeds were irradiated using a 100 MeV Linear Accelerator (TR103) at the Korea Multi-Purpose Accelerator Complex for PBs and a 60Co gamma irradiator at the Advanced Radiation Technology Institute for GRs, with doses of 50, 100, 150, 200, 250, 300, 400, 500, 600, and 700 Gy in both cases. The PB irradiated seeds did not germinate from 500 Gy or higher doses, whereas the GR irradiated seeds did not germinate from 600 Gy or higher doses. The dose showing a half germination rate against the control were 200 Gy and 350 Gy for PBs and GRs, respectively. The median lethal dose (LD50) and the median reduction dose (RD50) values estimated by analysing the survival rates and growth of the four-week-old seedlings were 150 Gy and 175 Gy for PBs, and 250 Gy and 300 Gy for GRs, respectively. These results suggest that the biological damage of PBs is more severe than that of GRs at the same dose. To induce mutations using PBs in rice, doses from 100 to 150 Gy seem to be proper. Now we are growing four large scale M 1 populations, with irradiation doses of 100 Gy and 150 Gy for PBs and 170 Gy and 250 Gy for GRs, to make mutagenized lines and subsequent genomic analysis. *Corresponding Author: Tel , hichoi@kaeri.re.kr 71

128 PA-98 Hyun min Kim *, Ga-young Lee, Ah young Kim, Sang hoon Ma, Seo young Park, Mai Thanh Dat, Ju hui Do, Chul-ho Yun, Young hee Joung * Plant NADPH-P450 reductase (CPR) is essential protein plays a role in transferring electrons for the catalytic reaction of P450 and is known as a key enzyme that regulates the P450 reactions. We identified several P450 genes involved in productivity through previous studies and isolated the CaCPR1 gene from hot pepper(capsicum annuum L. cv. Bukang) to regulate the reactions of the these P450 genes. To investigate the enzymatic properties, the CaCPR1 gene was heterologously expressed and purified in Escherichia coli. The enzymatic properties of CaCPR1 was confirmed by measurement of characteristic absorption spectrum and catalytic activities, which were assessed using protein and chemical substrates including P450, cytochrome c, cytochrome b 5, MTT, and CTC. In particular, CaCPR1 could support abscisic acid 8'-hydroxylation of purified plant CYP707A70 (ABA 8 -hydroxylase) better than rat CPR and FDX/FDR. These results reveal that the overall enzymatic properties of CaCPR1 is quite similar to those of typical CPR enzymes from other sources that have proven to be of utility value and indicate that CaCPR1 is better suited to promote the reactions of plant P450s than other sources of CPRs. *Corresponding Author: HM Kim, Tel , tblinj@naver.com YH Joung, Tel , yhjoung@jnu.ac.kr PA-99 권순욱 1, 박소연 1, 김동용 2* 1 2 춘란 (Cymbidium goeringii) 은 3~4 월에개화하는반음지성식물로서한국, 중국, 일본, 대만등의온대기후를갖는동아시아에자생하고있다. 수입되는동양란을대체하고경쟁력있는한국춘란품종을육성하고자개발된 색동이 품종의잎길이는 14.5cm 로형태적으로소형심비디움에속한다. 육성과정은 2004 년중국춘란과한국춘란을교배하여 2007 년복륜무늬를발견하였고, 2008 년까지기내고정후 2010 년포장시험에서무늬와형질이고정됨을확인하였다. 해당품종은맑고은은한향을가지고있고, 신아때부터복륜이형성되어잎끝에백황색의복륜이들며, 꽃에두가지의색상이발현되는복색화로서향기와무늬, 복색을가지고있어수출용으로경쟁력이높을것으로기대된다. 본연구는농림기술기획평가원기술사업화지원사업 ( ) 의지원에의해수행되었다. * 교신저자 : Tel , kdy5724@naver.com 72

129 PA-101 이창민 *, 박현수, 백만기, 김춘송, 남정권, 이건미, 박슬기, 서정필, 조영찬 Pecos 는 tropical japonica 생태형의미국벼품종으로우리나라 temperate japonica 품종과다른농업형질특성을가지고있다. Pecos 는중원형입형특성에황갈색외영, 자색의주두, 부선, 호영을가지고있으며외영과잎에모용이거의없는특성을나타낸다. 우리나라육성품종에없는새로운형질특성을평가하고육종사업에활용하기위해서 Pecos 를부본으로하고우리나라자포니카초다수품종인 보람찬 을모본으로인공교배하여단립종자후대법으로 F 7 세대 187 개재조합자식계통을육성하였다. 모 부본과재조합자식계통에대한출수기, 간장, 수장, 수수및입형관련형질을조사하였고, 소수관련형질인외영의모용성과색, 주두, 부선, 호영의색을조사하여유전분리비를분석하였다. 보람찬과 Pecos 의파종후출수기까지의기간은 109 일과 99 일이었으며, 재조합자식계통집단은 일에분포하였고보람찬보다출수가늦은계통이 117 계통이었다. 간장, 수장, 수수는대부분정규분포하는경향을나타냈다. 보람찬은현미장폭비 1.61 의단원형, Pecos 는 2.18 의중원형입형특성을나타냈다. 재조합자식계통의입형관련형질은대부분정규분포하는경향이었으며, Pecos 와같이현미장폭비가 2.0 이상인중원형계통은 51 개로단원형입형의계통이더많이존재하였다. 낟알의길이는낟알의두께, 장폭비, 천립중과정의상관을나타냈고, 낟알의두께는장폭비와부의상관을, 장폭비는천립중과부의상관을나타냈다. 소수관련형질의형태학적특성은보람찬은모용성이있는황백색외영에주두는백색, 부선과호영은짚색이었고, Pecos 는모용성이거의없는황갈색외영에주두와부선, 호영은자색이었다. 재조합자식계통의외영색, 주두색, 부선색은보람찬과 Pecos 형으로 3:1 의분리비를보여 Pecos 의형질특성은하나의열성유전자가관여하는것으로판단되었다. 형질들의관련성을보면외영의모용성은외영색, 주두색, 부선색, 호영색과는연관되었고, 주두색은부선색및호영색과연관되어있는것으로나타났다. 보람찬과 Pecos 유래재조합자식계통은우리나라자포니카품종과다른형태학적특성을가지고있어육종적가치를평가하기위해향후정밀한특성조사가필요할것으로생각된다. * 교신저자 : Tel , cropas@korea.kr 73

130 PA-102 김민주 1*, 박재호 1, 윤수현 1, 진성범 1, 박석만 최근우리나라의분화 (pot-flower) 경매거래량에따르면주로관상용으로이용되는분화거래량은꾸준한증가추세를보이고있으며, 그중거래되는과수분화류는감귤과레몬이유일하다. 따라서농촌진흥청에서는관상용레몬을육성하여관상용레몬소비를촉진코자관상용레몬 미니몬 을육성하였다. 미니몬 은 Maiyer 레몬의자연교잡우연실생으로기존 Maiyer 레몬과비교했을때과실크기가작고둥근모양이기때문에관상용으로적합하고식용으로도이용할수있다는것이가장큰특징이다. 미니몬 은 2006 년교배가된후 2007 년에종자를채종한후에시설에서종자를파종하고육묘를하였으며 2009 년봄탱자나무에고접을하였다. 이후 2013 년도에첫결실이이루어져서 2016 년까지특성조사를실시한결과우수하여 1 차선발하였고, 2017 년최종선발하여직무육성신품종심의회에서신품종으로선정되었다. 미니몬 은수세가중간정도이고나무자세도개장성이며, 과실은동그란구형이고열매크기는 40g 정도로매우작은편이다. 겉껍질은 2.6mm 정도로기존레몬에비해다소얇은편이다. 자가화합성이고평균 9 개의종자가형성되며형성된종자의배는다배성을나타낸다. 1 차개화시기의수확기과실당도는 8.7 Brix, 산도 5.00% 로대조품종과비슷한수준을나타낸다. 가지에작은가시가발생되나유년성이타파되면서가시가없어지는경향을나타낸다. 미니몬 의새순은 4 월중순에발아되고, 꽃은대부분 5 월에개화되는데사계성이있다. * 교신저자 : Tel , 88minju@korea.kr 74

131 PA-104 So-Yeon Park 1, Heng Wang 1, Da-Eun Im 1, San Mar Lar 1, Seong-Gyu Jang 1, Sang-Hyeon Jin 1, Ja-Hong Lee 1, Soon-Wook Kwon 1* 1 Direct-seeding cultivation is getting important to solve the problems such as water shortage, labor scarcity and cost down in all over Asia, including Korea. Low temperature germination and mesocotyl elongation are important traits for expanding of direct seeding cultivation. In this study, We evaluated elongation ability of mesocotyl, and performed GWAS analysis on core collection of 137 cultivars. Elongation ability of mesocotyl was appeard that average 3.2mm in Indica type, average 3.4mm in Japonica type and average 23mm in Aus type. In result of GWAS analysis, significant SNP was explored for elongation ability of mesocotyl on the chromosome 1, 3, 4, 8, 11. We performed haplotype analysis of candidate genes. These results will be able to use for development of suitable cultivar in direct-seeding. This study was performed by support of National research foundation of Korea (NRF-2015R1C1A1A ). *Corresponding Author: Tel , swkwon@pusan.ac.kr PA-105 김현태 1*, 강범규 1, 서정현 1, 이영훈 2, 이병원 3, 서민정 3, 고종민 1, 윤홍태 3, 신상욱 1, 김홍식 1, 곽도연 쌀생산조정에따라논에서의콩재배가증가하고있으며콩의재배가규모화, 집단화함에따라기계화의필요성이증가하고있다. 또한, 논재배에서배수불량에따른습해와함께뿌리썩음병이발생하기도한다. 이에식량과학원에서는병에강하고기계수확이가능한콩 평원 을개발하였다. 병과재해에강한육성계통 SS98205 를모본으로하고, 다수성인 대망 2 호 를부본으로 2008 년에인공교배하여 F 3 세대까지집단양성한후 F 4 부터계통으로전개하여선발하였다 년도에실시한생산력검정시험에서는다수성으로유망시되어 밀양 288 호 의계통명을부여한후 2015 ~ 2017 년 3 개년간전국 11 개소에서지역적응성을검정하였다. 평원 은유한신육형으로모용은회색이고, 협색은갈색이며종피와배꼽색은황색이고꽃색은백색이다. 개화기는대원콩보다 7 일늦으나성숙기는비슷하며, 경장이대원콩대비 13cm 짧은반면지면에서가장낮은꼬투리까지의높이가 4cm 더높으며, 100 립중이 22.8g 으로대원콩보다 3.6g 가벼운중립종이다. 불마름병에중도저항성이며검은뿌리썩음병에대한이병주율이낮다. 콤바인수확적성관련형질인도복에강하고, 협고가 14cm 로비교적높으며, 건조기를이용한협개열성조사에서탈립이되지않아기계수확이용이한품종으로생각된다. 메주수율은낮으나두부와청국장제조수율이높으며전국평균수량은 3.66ton/ha 로 대원콩 보다 10% 증수한다. * 교신저자 : Tel , sojatae@korea.kr 75

132 PA-106 Youra Hwang, Hee-Seung Choi, Hyun-Min Cho, Hyung-Taeg Cho * ROOT HAIR SPECIFIC (RHS) genes, which contain the root hair-specific cis-element (RHE) in their regulatory regions, function in root hair morphogenesis. Here, we demonstrate that an Arabidopsis thaliana basic helix-loop-helix transcription factor, ROOT HAIR DEFECTVE SIX-LIKE 4 (RSL4), directly binds to the RHE in vitro and in vivo, up-regulates RHS genes, and stimulates root hair formation in Arabidopsis. Orthologs of RSL4 from a eudicot (poplar, Populus trichocarpa), a monocot (rice, Oryza sativa), and a lycophyte (Selaginella moellendorffii) each restored root hair growth in the Arabidopsis rsl4 mutant. In addition, the rice and Selaginella RSL4 orthologs bound to the RHE in in vitro and in vivo assays. The RSL4 orthologous genes contain RHEs in their promoter regions, and RSL4 was able to bind to its own RHEs in vivo and amplify its own expression. This process likely provides a positive feedback loop for sustainable root hair growth. When RSL4 and its orthologs were expressed in cells in non-root hair positions, they induced ectopic root hair growth, indicating that these genes are sufficient to specify root hair formation. Our results suggest that RSL4 mediates root hair formation by regulating RHS genes and that this mechanism is conserved throughout the tracheophyte (vascular plant) lineage. *Corresponding Author: htcho@snu.ac.kr PA-107 송석보 1*, 고지연 1, 우관식 2, 정태욱 1, 최명은 1, 김정인 1, 고종철 3, 문중경 4, 박장환 1, 최유미 4, 곽도연 홍다 는기계화적성이우수하고기능성이뛰어난팥품종을육성하고자 2008 년하계에국립식량과학원에서쓰러짐에강한재래종 IT 를모본으로하고적색이고대립인일본수집종 K 을부본으로인공교배하여계통육종법으로선발한품종이다 ~ 2014 년생산력검정시험에서쓰러짐에강하고수량성이높아 밀양 30 호 로계통명을부여하였다 ~ 2017 년지역적응시험을실시한결과, 기능성이우수하고내재해성품종으로인정되어 2017 년농작물직무육성신품종선정위원회에서신규등록품종으로결정되었다. 홍다 의개화일수는 46 일로충주팥보다 7 일정도빠르고생육일수가 99 일로 5 일빠른중만생종이다. 경장은 61 cm이며협당립수는 7.1 개로충주팥보다많고쓰러짐에강하다. 100 립중은 14.1g 으로맑은적색의종피색을가진품종이다. 통팥가공적성이우수하고앙금수율이높아팥가공제품제조시품질면에서유리하다. 수량성은지역적응시험에서평균수량이 2.10MT/ha 로다수성품종이다. 적응지역은강원도산간고랭지를제외한전국팥재배지역에서재배가가능하다. 기계수확이가능한 홍다 는노동력과생산비절감으로생력화와농가소득증대에기여할것으로기대된다. * 교신저자 : Tel , songsb1254@korea.kr 76

133 PA-108 이형운 *, 남상식, 강용구, 양정욱, 유경단, 한선경, 고산, 이경보, 황엄지 고구마는가뭄등불량환경에대한적응성이뛰어난작물로알려져있으나최근고구마정식시기의가뭄지속, 병해충피해등으로인해단위면적당생산량이감소하고있다. 이러한고구마생산량감소에대응하기위해환경스트레스에내성이강한다수성품종육성이필요하다. 가뭄등환경스트레스에내성이강한다수성품종육성을위한교배모 부본자원을확보하기위하여페루에위치한국제감자연구소 (International Potato Center) 와공동으로본연구를수행하였다. 국제감자연구소보유유전자원중에서고구마주요병해충인덩굴쪼김병, 선충에저항성반응을보이는 5 개자원을교배부본으로선정하였고, 괴근수량이양호한 90 개자원을모본으로선정하여인공교배를하였으며 284 조합 7,999 립을채종하였다 년과 2016 년에교배종자를발아시켜국립식량과학원바이오에너지작물연구소내육묘상에서육묘하였고실생개체선발시험을통해괴근의비대정도가양호한 86 개계통을선발하였다 년에는 86 개계통으로계통선발시험을수행하여대비품종보다상품괴근수량이많거나포장에서의생존율이높은 18 개계통을선발하였다. 이중 등 3 계통은대비품종보다상품괴근수량이 55% 내지 107% 더많았다. 선발된계통들은내재해, 다수성고구마계통육성을위한교배자원으로활용할예정이다. * 교신저자 : Tel , leehu79@korea.kr PA-109 이덕렬 1*, 조승현 1, 송영은 1, 권석주 1, 이인석 1, 이송이 1 1 흑미주산지인전북지역에적합한고품질유색찰벼품종육성을목적으로 2006 하계에전라북도농업기술원에서 JR 를모본으로하고흑향벼를부본으로인공교배하여 F 3 이후부터는계통육종법에의하여육성선발하면서내도복성및색택검정을실시하여초형이직립이면서도복에강하고품질이양호한드림흑향찰을개발하였다. 드림흑향찰 은출수기가 8 월 18 일로 조생흑찰 보다 17 일늦은중만생종이며, 벼키는 67cm 로조생흑찰과비슷하나좌절중이무거워쓰러짐에강하고수발아율은 0.6% 로조생흑찰 11.6% 보다낮다. 드림흑향찰 은벼흰잎마름병과줄무늬잎마름병에약하고해충및바이러스에저항성이없다. 현미수량성은 년 3 년간실시한지역적응시험보통기보비재배에서 5.05MT/ha 로조생흑찰에비해 11% 정도많으며, 제현율은 80.5% 로조생흑찰 79.8% 보다높고향이있다. 드림흑향찰의적응지역으로는전북평야지 1 모작에적합이다. * 교신저자 : Tel , ldr0128@korea.kr 77

134 PA-110 김성훈 1*, 조규택 1, 마경호 1, 이정로 1, 이기안 2, 신명재 1, 세바스틴 1, 왕샤오한 농촌진흥청국립농업과학원농업유전자원센터의주요업무중의하나는자원접수및임시번호부여이다. 특히최근강화된기탁절차를숙지하지못하고국외에서우수한도입자원을가지고오는경우가존재한다. 우수한종자라도기탁절차에필요한서류를완벽히갖추지못하면농업유전자원센터에접수하는것은어렵다. 그래서농업유전자원센터의기탁절차를알리는것은물론 2017 년부터 2018 년까지농업유전자원센터에기탁된자원현황에대해서알리고자한다. 기탁자원접수현황은다음과같다. 17 년은 93 건 4,346 자원을접수했다. 밀, 동부, 병아리콩등 123 작물을입고되었고임시번호는 K261321~K 를부여했다 년 1 분기자원접수현황은 6 건 819 자원이다. 임시번호는 K266048~K 이주어졌으며벼, 밀등 54 작물이다. 2 분기자원접수현황은 9 건 604 자원이다. 임시번호는 K266867~K 이주어졌으며콩녹두등 15 작물이다. 임시번호를부여받은자원들은등록심의회를거쳐서 IT 번호를부여받을수있다. 학명, 자원명, 원산지, 자원구분등기초정보를충분히갖추고, 보존되어있는등록 (IT) 자원과중복되지아니하며등록기준량및발아율을만족하는자원이 IT 번호를부여받는조건이다. 기탁받은작물은식량작물, 원예작물, 특용작물, 기타작물순으로식량작물이가장높은비중을차지한다. 그러나최근에는원예, 특용작물의활용도도높아지고있으므로원예, 특용작물유전자원의확보에집중할필요성이있다. 농업유전자원센터에서작물의다양성을보다확보하여농업의발전에더욱이바지해야할것이다. * 교신저자 : Tel , shkim0819@korea.kr PA-111 Yuan Cao 1, Sang-Ho Chu 2, Kyu-Won Kim 2, Yong-Jin Park 1,2* 1 2 Betaine aldehyde dehydrogenase (BADH) is a key enzyme involved in the synthesis of glycine betaine (GB), a powerful osmoprotectant against salt and drought stress which found in many plant species. Our previous study found that the expression of betaine aldehyde dehydrogenase 1 gene (BADH1), encoding a key enzyme for the glycine betaine biosynthesis pathway, showed a close correlation with the salt tolerance of rice. Rice (Oryza sativa L.) is thought to be a non-accumulator of GB, but it does express BADH at low levels. In this study, we scanned the BADH1 sequences of 421 rice cultivars, and 54 wild rice accessions to determine their polymorphisms, gene functions and domestication. A total of 44 alleles for BADH1 were detected in transcribed regions of cultivars and wild species, while 2 alleles (exon 4 present T/A and exon 11 present A/C) showed high correlation with salt tolerance. These results suggest that the BADH1 gene could be an excellent candidate for rice functional research and breeding. *Corresponding Author: Tel , yjpark@kongju.ac.kr 78

135 PA-112 신명재 1*, 이기안 2, 김성훈 1, 마경호 1, 이정로 1, 옥현충 1, 조규택 농업유전자원센터는농촌진흥청산하의연구기관으로, 미래의식량전쟁을위한보루이자, 육종산업의발전을위한기반시설이다. 식물자원중종자를중심으로국가등록이된 IT 자원을직접보존하고있으며, 총 1,590 종 224,926 자원의식물 ( 종자 ) 유전자원을보존, 평가및분양을통해식물육종학의발전에이바지하고있다. 유전자원센터에서는유전자원의관리에많은예산과인력이소모되기에보존안전성에높은활용성을추가하기위해분양트렌드를파악하였다 년 ~2017 년에자원보존을위한증식분양 210,786 자원을제외하고분양된 390,416 자원을기준으로하였다. 육종을포함한식물소재산업의발전을위한분양은총 385,042 자원이분양되었고, 교육 전시및기타는 5,374 자원이분양되었다. 연도별로는 2000 년대에비해 2010 년대에연구목적분양이급격히증가하였으며, 5 년전과비교하였을때, 식량작물은 116%, 원예작물은 140%, 특용작물은 184% 증가하였다. 이러한현황을보았을때, 식물소재산업의발전을위한유전자원의활용은뚜렷하게증가하고있으며, 그중에서도특용작물 > 원예작물 > 식량작물순이다. 분양트렌드를통해, 육종을포함한식물산업내의관심과앞으로의발전방향을예측할수있으며, 그결과특용작물에대한관심과가능성을알수있다. 그러므로활용이전제로된보존을위해특용작물유전자원의확보및관리에집중할필요성이있으며, 이를통해식물소재산업의발전에더욱더이바지해야할것으로판단된다. * 교신저자 : Tel , smj1204@korea.kr PA-113 Lin Cheng 1, Kyu-Won Kim 2, Jungrye Nam 2, Yong-Jin Park 1,2* 1 2 Cultivated rice indica and japonica are the main rice crops of the world. The domestication process of rice is complicated. So far, extensive research on the origin of rice has been carried out, but they have not drawn a unified conclusion on the origins of cultivated rice. Recently, we have found interesting results supporting the independent origin of indica and japonica base on a phylogenetic research of rice chloroplast genome. Here, A total of 475 rice samples were collected from 28 regions of the world's rice-rich areas sequencing with a high average coverage (~15.88X), product ~3.42T raw data. We identified 1286 SNVs and 156 InDels in chloroplast genome among the whole samples. In order to have a more comprehensive understanding of cultivated rice and wild rice, we also classify those rice group in to 29 subgroups. The phylogenomic studies showed that japonica and indica were clearly separated. Phylogenetic analysis identified specific selection markers in different regions of their chloroplast genome. This indicates that different selection characteristics may be processed for indica and japonica during domestication. *Corresponding Author: Tel , yjpark@kongju.ac.kr 79

136 PA-114 Jee-Su Kang 1, Jungrye Nam 2, Kyu-Won Kim 2, Yong-Jin Park 1,2* 1 2 Bacterial blight (BB) caused by Xanthomona oryzae pv. oryzae (Xoo) is one of the major diseases of rice. It occurs in most areas that cultured rice, such as Africa and Asia. In particular, BB causes serious damage to rice cultured in Southeast Asia. Depending on the growing season, it can causes 20-50% reduction in yield, and it also decreases the quality of the rice. To date, about 39 genes are known to be resistant to blight, nine of which are cloned (Xa1, xa5, Xa10, xa13, Xa21, Xa3/Xa26, Xa23, xa25 and Xa27) and six of which are mapped (Xa2, Xa4, Xa7, Xa30, Xa33, and Xa38). Using this knowledge of genetic resistance, we analyzed the haplotype diversity in an effort to develop new BB resistant cultivars. We used the genomic information of 475 key clusters of Korean rice genetic resources including 54 accessions of wild rice. Our analysis of the mutation information of the BB resistance gene found that a large number of SNP and indel mutations exist in the BB resistance gene of the domestic core resource. We extracted alleles of MAF > 0.05 from these mutations and classified them into haplotypes. *Corresponding Author: Tel , yjpark@kongju.ac.kr PA-115 Rungnapa Phitaktansakul 1, Sang-Ho Chu 2, Kyu-Won Kim 2, Young-Sang Lee 3, Yong-Jin Park 1,2* The traits of fragrant rice (Oryza sativa L.) are attractive to consumers. Fragrant rice has a huge economic importance and provides a premium price in global trade. Fragrance in rice results from the loss of function of betaine aldehyde dehydrogenase (Badh2) gene on chromosome 8. In this study, whole-genome sequencing data of 475 rice germplasms reveals Badh2 gene in 49 cultivated and 31 wild rice accession numbers. Thirty-nine alleles in exon region of badh2 were detected in the sequence data. The novel allele (badh2-e2.22) of a single accession in cultivated rice was present one SNP (C/A) in exon 2. In wild rice, five SNPs novel alleles (badh2-e2.23, badh2-e3.24, badh2-e6.25, badh2-e10.26, and badh2-e13.27) were present C/G in exon 2, exon 3 present G/C, exon 6 present A/G, exon 10 present G/A, and exon 13 found A/T respectively. We developed the new functional SNP marker using primers and TaqMan probes for detection of SNP (C/A) in the badh2-e2.22 allele with real-time PCR. Twenty-one fragrant and non-fragrant rice varieties were selected to confirm the presence of the novel allele. The SNP marker reliably distinguished between all fragrant and non-fragrant genotypes and showed perfectly allelic discrimination plot between allele1 (A) and allele2 (C) in 20 rice accessions. Detection of this marker is a rapid and precise way to identify SNP fragrance rice and will be used in a Badh2 diversity study to improve the breeding of new fragrant rice varieties. *Corresponding Author: Tel , yjpark@kongju.ac.kr 80

137 PA-116 Ji-Min Yoo 1, Sang-Ho Chu 2, Kyu-Won Kim 2, Yoo-Hyun Cho 3, Yong-Jin Park 1,2* Granule-bound starch synthase I (GBSSI) is an enzyme involved in amylose synthesis during starch biosynthesis and is encoded as a waxy gene. This gene which is a starch synthase (SS) isozyme bound to starch particles, is a functionally different gene from the SS involved in amylopectin synthesis. GBSSI plays a role in polymerizing sugar molecules produced by photosynthesis into amylose, a form of stored carbohydrate. We performed a mutational analysis of GBSSI to provide important background data for improving starch quality based on genome information in rice breeding. To investigate the polymorphisms and genetic distribution of GBSSI, we implemented variant calling using a total of 475 resequencing data including 54 wild rice and 421 cultivated rice. As a result, GBSSI (Os06g ) was located on chromosome 6, and a total of 4 mutations were confirmed in 16 Exons. Particularly in Exon 1, a 24bp mutation caused by a frameshift mutation and one non-synonymous SNP was observed. In Exons 5 and 8, many non-synonymous SNPs altering amino acid coding were observed. *Corresponding Author: Tel , yjpark@kongju.ac.kr PA-117 Jungrye Nam 1, Kyu-Won Kim 1, Ji-Hye Hwang 3, Sook Kim 4, Hyun Namgung 4, Young-Chul Jeon 4, Gang-Seob Lee 5, Jung-Kyung Moon 3, Yong-Jin Park 1,2* We designed array based high-throughput DNA markers with high specificity for GMO detection and identification. The information that could be used for the marker design was not formalized, and different methods were used depending on the type of data. Two main types of information were entered into the GMO-related databases. A pair of PCR primers was converted into the PCR product and its marker was designed. If the sequence of a transgenic plant was provided, the marker was designed after confirming the boundaries between the host sequence region and the vector sequence region. However, direct information for identifying GMO sequences was still limited, so we also designed a wide range of markers for vector sequences to greatly enhance detection coverage. From this more extensive vector sequence database, the sequences in the promoter and terminator regions were sampled. To select highly specific markers, we oversampled a larger number of sequences than the number of required markers, and selected the markers with the highest specificity by performing pairwise sequence alignments. *Corresponding Author: Tel , yjpark@kongju.ac.kr 81

138 PA-118 Kyu-Won Kim 1, Jungrye Nam 1, Yong-Jin Park 1,2* 1 2 We analyzed genetic diversity among 3,475 rice accessions consisting of 58 accessions of wild rice, 417 accessions of the Korean rice core set (KRICE_CORE), and 3,000 accessions of Asian cultivated rice (by CAAS, BGI and IRRI). We performed whole-genome genotyping based on whole genome resequencing from 58 accessions of wild rice and 417 accessions of the KRICE_CORE. We extracted integrated high quality SNP/indel variant sites from 475 and 3,000 rice accessions. We identified linkage distribution, genetic diversity, and haplotype blocks in 3,475 accessions by using the variants, and the allelic and genic differentials between populations such as indica/japonica and wild/cultivated. *Corresponding Author: Tel , yjpark@kongju.ac.kr 82

139 PA-119 Kyu-Won Kim 1, Jungrye Nam 1, Ji-Min Yoo 2, Sang-Ho Chu 1, Rungnapa Phitaktansakul 2, Jee-Su Kang 2, Myeong-Hyun Min 2, Lin Cheng 2, Yuan Cao 2, Sang-Beom Lee 3, Ji-Hye Hwang 4, Sook Kim 5, Hyun Namgung 5, Young-Chul Jeon 5, Soo-Cheul Yoo 6, Joo-hyun Lee 7, Soon-Wook Kwon 8, Joong-Hyoun Chin 9, Ki-Hong Jung 10, Young-Sang Lee 11, Nam-Chon Paek 12, Gang-Seob Lee 13, Yoo-Hyun Cho 14, Sang-Won Park 3, Il-Pyung Ahn 15, Hyeon-So Ji 16, Seung-Hyun Kim 7, Byeong-Chul Moon 3, Hee-Jong Koh 12, Jung-Kyung Moon 4, Ill-Min Chung 7*, Yong-Jin Park 1,2* We designed a number of candidate DNA markers for the 833K rice genotype DNA array to be used for agricultural researches such as GS, GWAS, map-based cloning, subspecies specific gene analysis, plastid genome analysis, evolutionary study, and GMO identification. The SNP/indel site information to be used for the development of the array markers were collected from an approximate total of 3,500 rice accessions, including Asian cultivated rice, KRICE_CORE, as well as wild rice. We classified the markers into two major groups for genome-wide genotyping and the case study. The markers for the SNP/indels of the entire genome were designed within the genome-wide genotyping group, but the minor variants (alleles) of low frequency were filtered. In the case study group, the markers were divided into 5 subgroups according to research purpose, absent any minor allele filtration. The first group consisted of markers for a genetic diversity study on nucleic and plastid genomes, targeting both cultivated rice and wild rice. The second group consisted of markers for studying the diversity of proven genes shown to be key to major agronomic traits. The third group is for breeders consisting of markers covering genes in a wide range pertinent to traits favorable to breeding, such as eating quality, yield, and disease resistance. The fourth group consisted of markers for metabolic pathway analysis. The fifth consisted of markers for GMO identification. *Corresponding Author: (Yong-Jin Park) Tel , yjpark@kongju.ac.kr (Ill-Min Chung) Tel , imcim@konkuk.ac.kr 83

140 PA-120 신동진 1*, 김경훈 1, 김경민 1, 차진경 1, 김영진 1, 고종민 1 1 국내밀재배면적은 1 만 ha 내외로자급율은 2% 이하다. 자급율향상을위한밀재배면적확대가중요하나, 밀적정파종시기인 10 월중하순에잦은강우로파종을하지못하는경우가발생한다. 위를해결하고자국내육성 42 품종등총 1,117 자원을추파 (10 월 24 일 ) 와춘파 (2 월 9 일 ) 로파종하고춘파재배적응성을비교검정하였다. 국내육성품종 42 점중 영광 등 6 개품종은파성이높아춘파재배에서는줄기형성이지연되거나지연되어춘파용품종으로는적당하지않았다. 조경 등 36 개품종이성숙기가 6 월 10 일이전으로수확이 6 월 15 일이전에가능하였다. 특히 조품 과 조농 은춘파재배시출수기가 5 월 4 일과 8 일로다른품종보다빨랐으며, 조경 과 수안 이상대적으로초형이다른품종보다양호하였다. 하지만, 추파재배보다춘파재배에서국내육성품종의간장은평균 7.5cm(10%) 감소하였다. 국내육성품종이외의유전자원중에 회계 2009, 중계 4899 등중국도입의유전자원이출수기가 5 월 10 일이전으로빠른편이였다. 도입유전자원중 CHN 과 BECARD/KACHU 은직립형이며간강이 70cm~80cm 로상대적으로초형이우수했다. 특히, ICW K-1AP-0AP-4AP-2 은직립형이며수층이상대적으로균일하게위치하여품질이안정적인품종육성을위한유전자원으로활용할수있을것이다. 또한 ISENGRAIN 등의유전자원은파성이높았으며, 이들유전자원은파성이낮은유전자원보다뿌리신장이약 1.5 배길었다. 이는파성이높은유전자원이내한발성을포함한재배안정성이우수함을의미하며, 추후내한발성중간모본개발을위한부본으로활용할수있을것이다. * 교신저자 : Tel , jacob1223@korea.kr PA-121 Young-Chan Cho *, Man-Ki Baek, Hyun-Su Park, Jong-Min Jeong, Chun-Song Kim, You-Chun Song, Jun-Hyun Cho, Ji-Yun Lee, Bo-Kyeong Kim, Jeom-Ho Lee This study was tried to develop the long grain indica rices adaptable to tropical southeast Asia regions in Cambodian Agriculture Research and Development Institute (CARDI), Cambodia. The final goal is to develop rice varieties and export rice seed which can culture in diverse environmental conditions of tropical regions of southeast Asia under climate change. Using rice germplasm from Cambodia, India, Indonesia, Myanmar, Philippines and Vietnam in CARDI, there were developed 1,450 F1 cross combinations, and selected 1,045 lines in 74 cross combinations in F4 subsequent generation. We could select better lines of clear and translucent long grain from the cross combinations with aromatic varieties of Phka Rumdoul, Senpidao and Senkra Ob in Cambodia varieties, Jasmine85 in Thailand variety, and Basmati370, Pusa Basmati1, Sharbati and Sugandha in India varieties. We developed three promising lines of days of the growth duration from sowing to harvesting. These lines were evaluated for adaptability in three regions in Vietnam, and one region in Laos with IR66 as standard check variety. A aromatic line KR was higher 2 7% than that of IR66 except to Longan region in Vietnam. KR showed higher 16% than that of IR66 in Laos. KR was higher 33% than that of IR66 in Dong Nai region, Vietnam. The yields of these lines were higher than those of the leading varieties of OM4900 and OM5451 in Vietnam, and TDK8 in Laos. The percentage of ripened grains was important component traits for yield in dry season of tropical regions in this study. These results mean that heat stress was influenced to increase sterility and decrease the grain filling in dry season. In future, we will try to select the lines of heat tolerance in flowering time and high grain filling in ripening period. *Corresponding Author: Tel , yccho@korea.kr 84

141 PA-122 햠 조다님 1, 김민수 1, 하보근 2, 강성택 3, 송종태 4, 이정동 1* 콩의지방은일반적으로 5 가지지방산인 palmitic(16:0) 12%, stearic(18:0) 4%, oleic(18:1) 23%, linoleic(18:2) 53%, 및 linolenic acid(18:3) 8% 로구성되어있다, 그중 Oleic acid 에서 linolenic acid 로의생합성에관여하는 Fad2-1A 와 Fad2-1B 유전자가기능을잃을경우 oleic acid 가높아지는것으로보고되었다. 국내에서육성된재배콩광안은 FAD2 유전자에돌연변이없이약 40% 의 oleic acid 함량을보이는것으로보고되었으나, 이함량을조절하는 QTL mapping 연구는진행되지않아본연구에서실시하였다. 정상적인 oleic acid 함량을가진재배콩 5002T 와 oleic acid 함량이약 40% 인광안을교배한뒤 2016 년경북대학교군위실험장에서 F 6:7 세대 150 개의 RILs 를육성하였다. RIL 의유전자형은각각의 RIL 종자에서 DNA 를추출한후 Soybean 6K SNP chip 을이용하여확인하였다. RIL 의지방산변이를검정하기위해 2017 년에 4 개의환경에서 2 반복씩파종하였다. 재배환경별파종시기는경북군위에서 5 월 26 일과 6 월 20 일, 전남광주에서 6 월 23 일, 충남천안에서 6 월 3 일에파종을하였다. 지방산함량은 GC 를이용하여분석을하였으며, 염색체지도와 QTL 분석은 'ICIMapping' 프로그램을이용하여실시하였다 년 4 개의환경에서수확한 150 개 RILs 과양친의지방산함량을분석하였다. 양친의 oleic acid 의함량은광안콩이평균 39.7% ± 2.7 의결과를보이고, 5002T 이평균 27.8% ± 6.4 의결과를보여기존연구와같이광안콩에서높은함량을확인할수있었다. 150 개 RILs 에서의 oleic acid 함량은 20.7 ~ 40.4% 의범위를보였으며, 평균함량은 29.9% ± 3.8 로일반적으로알려진 23% 에비해높게조사되었다. 150 개의 RILs 는관찰된평균과분산을기준으로정규분포를구성하는것으로조사되었다. 조사한표현형과유전자형을토대로 ICIMapping 프로그램을이용하여 QTL 분석을한결과 13 번염색체에서한개의유의적인 QTL 을확인하였고, PVE 값은 20.6% 인것으로나타났다. 이에새로발견된 locus 를확인하는연구를진행하고있다. * 교신저자 : Tel , jdlee@knu.ac.kr PA-123 Ji-Min Yoo 1, Sang-Ho Chu 2, Yong-Jin Park 1,2* 1 2 Much research has been done on tocochromanols, also known as vitamin E, which play an important role in human and livestock health. Tocochromanols are metabolites synthesized via the vitamin E biosynthesis pathway and are affected by a number of genes involved in the synthesis pathway. Because of the importance of rice in the diets of many Asian populations, including Korea, studies on the genetic diversity of this crop related to the biosynthesis of tocochromanols have been carried out. In 475 accessions of the Korean rice core set, we analyzed the genetic diversity of pivotal genes in the pathway of tocochromanols biosynthesis that are provided in the rice reactome database. This allowed us to gather information on its genetic diversity, enabling a more systematic identification of varieties associated with tocochromanols content. *Corresponding Author: Tel , yjpark@kongju.ac.kr 85

142 PA-124 Hyunseok Lee *, Won-Woo Jo, Solji Lee, Hyemin Lim, Wi-Young Lee, Jun-Won Kang This study was carried out to enhance seed productivity and secure genetic resources of Pinus densiflora for. multicaulis(dm). The characteristics of cone and seed produced by mating design between Pinus densiflora(d) and P. densiflora for. multicaulis(dm) were investigated. The highest number of cone scales (63.0) was obtained from self-pollinated(sp) DM clone B, P. densiflora for. multicaulis (DM-sp-B), whereas the lowest number of cone scales (44.7) was obtained from two hybrid pines including DM-A D-075 and DM-A D Both the female parents of the hybrids were DM-A. The highest seed production capacity(80.8) was obtained from open-pollinated(op) DM clone B, DM-op-B, and the seed production capacities of DM-op-A, DM-B DM-B, and DM-B D-0111 were 67.4, 66.5, 63.1, 55.4, and 53.8, respectively. The highest number of fertile scales (41.5) was obtained from DM-op-B and the lowest number of fertile scales (28.8) was obtained from DM-A D-075. The DM-B D-0111 showed high number of developed seeds(43.8) as well as the lowest number of 2 nd aborted ovules (5.2) and empty seeds (9). Although the DM-op-B showed the highest number of developed seeds(47.6), the number of empty seeds was, also, the highest(41.2). Therefore, the mating combination of DM-B D-0111 could be recommended for future breeding program to improve seed yield of DM. In addition, the results showed that there was a strong correlation between the NS and NFS and NS and SP (R = 0.89 and R = 0.83, respectively; both P <.01). *Corresponding Author: Tel , elvangood@korea.kr PA-125 이솔지 1*, 이현석 1, 조원우 1, 강준원 1, 임혜민 1, 이위영 1, 김판기 우리나라에서는북미와대서양연안에자생하고있는리기다소나무와멕시코만과대서양연안이원산으로알려져있는테에다소나무를도입하여, 교잡육종으로중간형질을나타내면서우리나라의기후풍토에적합한리기테다소나무를육성하였다. 본연구에서는원산지의기후대가서로다른이들 3 수종을공시재료로하였다. 공시수종은서울대학교광양연습림내에서비교적우수한생장을나타내는 47 년생리기테다소나무와이것의육종모수종 ( 育種母樹種 ) 인테다소나무 (49 년생 ) 와리기다소나무 (43 년생 ) 5 개체씩을공시하였다. 이를사용해흉고단면적의연간생장량및춘추재생장량을분석하였으며, 기상데이터를활용하여생장과기후변화간상관관계를분석하였다. 기상변수로는일별기온 ( 최고 / 평균 / 최저 ) 과일강수량을사용하였다. 그결과, 리기다소나무는상록침엽수의광합성이최대에도달하는 7 월의기온이낮은해에는연간흉고단면적생장량이감소하는음 (-) 의상관을나타냈으며, 흉고단면적을구성하는춘재의형성량은그보다앞선 6 월의기온이낮을수록감소하는경향을나타냈다. 이러한경향은테에다소나무에서도나타났으나, 7 월의강수량이많은해에는연간흉고단면적생장량이증가하는양 (+) 의상관을나타냈다. 한편리기테다소나무는 3 월기온이높은해에연간흉고단면적생장량이증가하는양 (+) 의상관관계를나타내고, 8 월의기온이낮은해에는연간흉고단면적생장량이감소하는음 (-) 의상관관계를나타내서교잡육종의모수가되는리기다소나무및테에다소나무와는상이한경향을타나냈다. * 교신저자 : Tel solji730@korea.kr 86

143 PA-126 정영민 1, 이윤영 1, 이영실 1, 이하얀 1, 손미연 1, 지현소 2, 조영일 1* 1 2 차세대염기서열분석기술의발달로유전체에서단일염기다형성 (single nucleotide polymorphism, SNP) 가대량으로발굴되고있다. SNP 분자표지는빈도수가높고공우성인특성이있어그유용성이높지만 genotyping 이까다로워활용성에제한을받았지만최근다양한기술이개발되어이러한점이극복되고있다. 이중대립유전자특이적인 primer 로 SNP genotyping 을수행하는 Kompetitive Allele Specific PCR(KASP) 방법은분석이용이하고 high-throughput genotyping 이가능하여많이활용되고있다. 본연구에서는국내자포니카벼품종에서 517 개 SNP 를선발하고이를종자산업진흥센터의대용량자동화분석시스템에서사용가능한 KASP 분자표지로제작하였다. 제작된 KASP 분자표지는자포니카벼품종 20 개에적용하여그성능을검증한결과전체 517 개중 510 개 (98.6%) 가증폭이되었으며이중 424 개 (82.0%) 가적어도한품종이상에서다형성을보였다. 따라서이들 KASP 분자표지는벼의유전지도작성, QTL 탐지, 유전자동정, 여교배등에다양하게활용될것으로판단된다. * 교신저자 : Tel , breedy01@efact.or.kr PA-127 Cheol Won Lee, Yong Weon Seo * Wheat can be classified into Spring and Winter types based on growth habit. In winter wheat, it requires an induction of a plant's flowering process by exposure to the prolonged cold, known as vernalization. The regulation of various vernalization genes and transcription factors work during vernalization i.e. the time when early development stages of wheat. To clarify an influences of vernalization on the early stage of wheat development, we set experimental groups with different exposure length under low temperature. Two Korean cultivars, Keumgang and Yeongkwang, which have different maturity were incorporated in this study. Plants were exposed different periods under the 4. To establish the relationship between spike development and vernalization related genes, we performed qrt-pcr. The expression of Vrn1 and VER2 which interacted with Vrn1 shows a particular trend relative to development of spike primordium. We also figure out genes interacted with VER2 through yeast two hybridization. The obtained results will provide valuable information to understand the role of vernalization wheat aimed at establishing the mechanism of wheat development. Acknowledgement: This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (2017R1D1A1B ) *Corresponding Author: Tel , seoag@korea.ac.kr 87

144 PA-128 장성율 1, 이운석 1, 김형석 1* 1 토마토의생산성및품질에있어과실의생중량및부피의변화는중요지표중에하나이다. 기존의방법으로, 과실의생중량측정은파괴적인방법을통한무게측정을통해서, 부피의경우버니어캘리퍼를통한측정을통해서수행된다. 이러한방식들은측정시파괴적인과실의수확이요구되거나, 측정과정에서의많은시간과노동력이소요될수밖에없어과실비대과정중의연속적인데이터획득에장애요인이되고있다. 본연구에서는스마트폰의카메라를통해획득된토마토과실의이미지를딥러닝기반의영상처리및기계학습기반의생중량, 부피예측모델을통한분석으로실측대비 98% 정확도를갖는분석방법을개발하였다. 개발된방법은두과정으로구분된다. 첫째, 스마트폰을통해획득된이미지를통해과실의과장및과폭을측정하는딥러닝모델생성과정, 둘째, 딥러닝을통해획득된과실의과장및과폭정보를통해과실의생중량및부피를예측하는기계학습모델생성과정. 본연구는총 800 개과실정보를토대로 540 개의정보는모델생성을위한학습정보로, 260 개의과실정보는생성모델의검증을위해활용되었다. * 교신저자 : Tel , hkim58@kist.re.kr PA-129 Ju-Young Choi 1, Soo-Jeong Kwon 1, Ki-Hyun Kim 2, Seong-Woo Cho 3, Yong-Gu Cho 1, Y. Yasui 4 and Sun-Hee Woo 1* Common buckwheat has the sporophytic self-incompatibility mechanism and that s why it has the ability to cross pollinate between two plants with different styles (the pin type and thrum type). The S supergene is thought to govern self-incompatibility, flower morphology and pollen size in buckwheat. Already, we have produced self-compatible buckwheat lines by an interspecific hybridization between Fagopyrum esculentum and F. homotropicum by embryo culture. The pollen size of F 1 plants produced by a cross between a pin type plant and the self-compatible plant was similar to that of the self-compatible lines and segregated together with flower morphology without exception. The pollen tubes of the self-compatible plants were compatible with styles of the pin plants but incompatible with the styles of thrum plants. But, the pollen tubes of thrum flowers were compatible with the styles of self-compatible plants. Also, the pollen tubes of pin flowers were incompatible with the styles of self-compatible plants. Already, from these results, we have reported a tentative genotype for heterostyle and homostyle flower types. Homomorphism was controlled by a single allele S h, while the pin/thrum-complex gene was governed by a single genetic locus S, with two alleles, S and s, which control Ss (thrum-type) as well as the ss (pin-type), respectively. Corresponding represents the case of a single locus S with three alleles, S h, S and s, and the phenotypes, homomorphic, pin and thrum. It can be characterized by relationship of dominance, S>S h >s. Using the two self-fertile lines, one is considered as the long-homostyle flowers and the other is considered as the short homostyle flowers. If the short-homostyle trait had arisen by recombination in the S supergene, its genotype would be considered to be GI s i p a/gi s i p a.the pollen tubes of the short-homostylous plant should be compatible with the styles of thrum plants. Also, the pollen tubes of short-homostylous plants should be incompatible with the style of long-homostylous plants, and the reciprocal cross also should be incompatible, because the genotype of long homostyle is gi s I p PA/gi s I p PA. Furthermore, the flower morphology of F 1 plants produced by the cross between cross and short homostyle flowers should be thrum or short homostyle and only short-homostylous plants should be produced by the cross between pin and short homostyle flowers. However, the compatibility or incompatibility of short homostyle flower was not clarified. So, we need to clarify the compatibility or incompatibility of the style of short homostyle flowers for the next step. *Corresponding Author: Tel , shwoo@chungbuk.ac.kr 88

145 PB-01 Jin-Kee Jung *, Eun-Jo Shim, Hyun-Jung Kim, Sujin Lee, Hye-Won Kim, Mi-Su An, Sun-Young Ko and Seung-In Yi The lettuce(lactuca sativa L.) is a major horticultural crop from the family Asteraceae (Compositae). The genus Lactuca consists of about 100 species, 3 of which Lactuca serriola L. (prickly lettuce), Lactuca saligna L. (willowleaf lettuce), and Lactuca virosa L. (bitter lettuce) are wild species sexually compatible with L. sativa (Lebeda et al. 2007, Ivan et al 2008). Lettuce cultivars are classified into horticultural types based on head and leaf shape, size, and texture. All lettuce cultivars are self-fertilizing diploids with 2n = 2x = 18 chromosomes (Ivan et al 2008). For the development of novel SNP marker, we performed GBS (genotype-by-sequencing) with 96 lettuce cultivars, stored in KSVS(Korea seed variety service), varieties for mining reliable SNP loci. Finally we mined reliable 17,877 SNP loci among total 276,462 SNP s matrix for development of novel SNP marker for lettuce variety identification. The statistic result for transition and transversion ratio showed Out of 173,599 transition SNPs, C>T is 87,112 and A>G is 86,487. A transvesion SNP revealed 102,744, C>G is 16,367, A>T is 35,764, A>C is 25,430 and G>T is 25,183 respectively. All of SNPs are evenly distributed in each 9 chromosome. The result of genetic relationships analysis in Lettuce (Lactuca sativa L.) based on 17,877 SNP loci using MEGA program classified as 3 groups. These sequence-based SNP loci using GBS technology will be useful for develop novel SNP marker and reliable database for variety identification related to seed dispute and distinctness, uniformity and stability (DUS) test for lettuce. Acknowledgement: This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Agri-Bio industry Technology Development Program, founded by Ministry of Agriculture, Food and Rural Affairs(MAFRA) ( ) *Corresponding Author: Tel , jinkeejung@korea.kr PB-02 Jun Hee Jung 1*, Jungmin Ha 1, Moon Young Kim 2, Jung-Eun Kim 3, Hokyoung Son 3 and Suk-Ha Lee Due to their high iron and folate contents, mungbean (Vigna radiata (l.) R. Wilczek) is one of the most important crops in South-East Asia. However, considerable amount of the yield is lost due to various diseases. In Korea, limited information is available in public databases regarding the diseases of mungbean. The aim of this study was to isolate and identify the pathogens causing a leaf spot disease in mungbean. In October 2017, there was an outbreak of a leaf spot disease in Seoul National University Suwon Experimental farm. The diseased leaf was surface sterilized and grown in 2% water agar for 5 days. Mycellium from water agar was transferred to potato dextrose agar. The Internal Transcribed Spacer (ITS) sequences indicated that four species Alternaria alternata, Plectosphaerella cucumerina, Stagonosporopsis cucurbitacearum and Fusarium equiseti were isolated. Out of the four species, we obtained conidia of Alternaria alternata and Plectosphaerella cucumerina. The two species were further identified using the sequence of ribosomal large-subunit D1-D2 regions. The sequencing result of confirmed the identification based on ITS region. Using the two strains isolated, in vivo and in vitro tests on to fulfil Koch s postulate on mungbean is currently ongoing. To the best of our knowledge, this is the first time that either of the species are known to cause diseases in mungbean in Korea. The isolated and identified pathogens can be used to identify qualitative trait loci and candidate pathogen resistance genes which can assist in breeding disease resistant cultivars. *Corresponding Author: Tel , junheejung@outlook.com 89

146 PB-03 Daun Kim 1, Byeogil Je 1, Byung Sup Kim 2, Hee-Jeong Jung 3, Ill-Sup Noua 3, Younghoon Park 1,4* Reductions in growth and quality due to powdery mildew (PM) disease cause significant economic losses in tomato production. Oidium neolycopersici was identified as the fungal species responsible for tomato PM disease in South Korea in the present study, based on morphological and internal transcribed spacer DNA sequence analyses of PM samples collected from two remote regions (Muju and Miryang). The genes involved in resistance to this pathogen in the tomato accession KNU-12 (Solanum lycopersicum var. cerasiforme) were evaluated, and the inheritance of PM resistance in KNU-12 was found to be conferred via simple Mendelian inheritance of a mutant allele of the PM susceptibility locus Ol-2 (SlMlo1). Full-length cdna analysis of this newly identified mutant allele (Slmlo1.1) showed that a 1-bp deletion in its coding region led to a frameshift mutation possibly resulting in SlMlo1 loss-of-function. An alternatively-spliced transcript of Slmlo1.1 was observed in the cdna sequences of KNU-12, but its direct influence on PM resistance is unclear. A derived cleaved amplified polymorphic sequence (dcaps) and a high-resolution melting (HRM) marker were developed based on the 1-bp deletion in Slmlo1.1, and could be used for efficient marker-assisted selection (MAS) using KNU-12 as the source for durable and broad-spectrum resistance to PM. *Corresponding Author: ypark@pusan.ac.kr PB-04 Na-Rae Jeong, In-Taek Hwang, Heejin Kim, Jae-In Chun, Jin-Ho Kang * Trichomes are hair-like structures on the aerial surface of many plant species. Trichomes are well characterized for their roles as physical barriers and chemical defense against herbivore attack. Here, we describe the characterization of a monogenic recessive mutant of tomato (Solanum lycopersicum) called inquieta (ini). All trichome types on ini plants showed distinct morphological defects (e.g., swelling) that are known to be associated with defects in the actin cytoskeleton. Genetic mapping experiments positioned the Ini locus within a 1.5 cm interval on chromosome 11 that contains the tomato homolog of the Arabidopsis ARPC2A gene, which encodes a protein involved in nucleating the polymerization of actin filaments. Use of ARPC2A as a molecular marker showed that this gene strictly co-segregates with the target locus in a mapping population of 135 F 2 plants. Reverse transcriptase (RT)-PCR and genomic PCR experiments showed that full-length ARPC2A is amplified in wild-type but not in the ini mutant. Flanking PCR and Southern blot analysis showed that the ini mutation corresponds to a complex ~6-kb insertion in the 5 th intron of ARPC2A. Expression of a wild-type ARPC2A in the ini mutant background restored normal trichome development. These results provide molecular evidence that altered trichome development in the ini mutant is caused by a defect in actin cytoskeleton formation. *Corresponding Author: Tel , kangjinho@snu.ac.kr 90

147 PB-05 안율균 1*, 안영은 1, 홍규현 1, 이관호 1, 우영회 1, 김옥례 2, 이준구 3, 황인덕 4, 채수영 토마토재배시가장문제시되는병중하나인풋마름병은 Ralstonia solanacearu 으로가지과작물을주요기주로하며세계여러지역에분포하는병이다. 우리나라의경우도비닐하우스재배가늘어가고연작하는작부체계가일반적으로점점풋마름병의피해가증가하는추세에있다. 현재풋마름병에대한대책으로대목을사용하고있으며, 토마토접목재배가점차적으로늘어나는추세에있다. 또한토마토는장기재배에대한요구도가커재배기간동안다양한불량환경및병해충을견디고재배후기까지초세를유지하는대목과기존에개발된대목들이풋마름병에감수성으로바뀌고있어서저항성대목개발이필요한실정이다. 본연구는식물생체정보센서를사용하여풋마름병저항성을조기에예측하고대목사용시풋마름병의저항성을조기에분석하는방법을설정하고자시험을수행하였다. 시험재료는풋마름병저항성대목에접목을한것과접목을한지않은접수을화분에파종후본엽 5 6 매가되었을때풋마름병균을접종하였다. 병원균의접종농도는 1x10 8 /ml CFU 로접종하였으며, 조사는식물생체정보센서 (Telofarm) 를사용하여물의흐름정도를측정하였다. 측정결과균접종후접수로사용한토마토품종의경우 3 일정도에서물의흡수가줄어들었으며저항성대목으로접목한재료는정상적인물의흡수를보였다. 이상의결과를볼때 Micro Sap Flow 는풋마름병의조기진단에활용할수있을것으로보인다. * 교신저자 : Tel , aykyun@korea.kr PB-06 Sais-Beul Lee 1, Tae-Heon Kim 1, Joo-Won Kang 1, Su-Min Jo 1, Jun-Hyeon Cho 1, Ji-Yoon Lee 1, Jong-Hee Lee 1, Yeong-Ho Kwon 1, You-Chun Song 1, Jong-Min Ko 1, Dong-Soo Park 1* 1 Rice stripe virus (RSV) is one of the major constraints which is transmitted by the small brown planthopper (SBPH; Laodelphax striatellus). Typical symptoms of RSV are chlorosis and weakness of newly emerged leaves, white and yellow spots, stripe and necrotic on leaves, necrotic and wilting leaves, as a consequence, plant growth decline and the contaminated plants are gradually die. (Takahashi et al. 1991). In our previous study, we screened 5 RSV resistant cultivar including Padi Adongumarat, Tung Ting Wan Hien 1, 02428, Erguailai, and Daw dam, which harbors different resistance allele with Stv-b i. In this study, RSV resistance Six varieties, which does not harboring Stv-b i, reported by Kwon et al. (2012) were used to identify novel source of resistance on rice stripe virus. Sequence analysis revealed that Daw Dam and Erguailai contain resistant allele of OsSOT1. Genotype analysis on Stv-b using six Sid markers in the qstv11 Z region represent that Daw Dam and Erguailai harbors qstv11 Z, which showed Daw Dam and Erguailai expected to have OsSOT1 gene as well as Stv-b. Finally, we expect three varieties, and Tung Ting Wan Hien 1 as the novel source of Rice Stripe Virus resistance. *Corresponding Author: Tel , parkds9709@korea.kr 91

148 PB-07 Marjohn Nino 1, Franz M. Nogoy 1, Me-Sun Kim 1, Sothea Ouk 1, Ju-Young Choi 1, Yu-Jin Jung 2, Kwon-Kyoo Kang 2, Yong-Gu Cho 1* 1 2 Bacterial blight, caused by Xanthomonas oryzae pv. oryzae, is a serious disease problem of rice causing damage to rice quality and yield. To understand the transcriptional gene network involved in resistance against Xoo, a whole-genome oligonucleotide microarray of two popular japonica rice Dongjin and Jinbaek were used to infer transcripts of inducible genes between compatible and incompatible interactions at 48 hour post inoculation. A large number of genes are more evident in the resistant cultivar, which is threefold higher than in susceptible plant. Up-regulation of genes with predicted functions in signaling and transcription signifies orchestration of defense signals and robust cellular reprogramming leading to incompatible interaction. To further identify genes crucial to immunity, 13 Xoo-DEGs of different protein class were cloned and overexpressed using CaMV 35S promoter into rice. Most of the overexpression plants displayed improved resistance when screened against Xoo Korean race K2. Elevated transcripts levels of several defense-related genes at the downstream of defense signal network also corroborate the phenotype reaction of the transgenic plants. ROS levels continuously magnified after inoculation which indicates robust cellular sensing necessary to initiate cell death. Moreover, expression assays revealed regulation of these genes by cross-communicating signal-transductions pathways mediated by salicylic acid. These collective findings revealed the complexity of key immune signaling conduits critical to mount full defense against Xoo in rice. This work was supported by a grant from the Next-Generation BioGreen 21 Program (PJ ), RDA, Republic of Korea. *Corresponding Author: Tel , ygcho@cbnu.ac.kr PB-08 Joung-Ho Lee 1, Jongwook Ahn 1, Jin-Kyoung Kwon 1, and Byoung-Cheorl Kang 1* 1 Chili veinal mottle virus (ChiVMV) is the virus causing severe losses of pepper yield mostly in Asia and Africa. Nevertheless, only one resistance locus (Cvr1) with several Cvr1-linked markers have been reported up to date. In this study, we precisely mapped the single dominant resistance locus, Cvr1, in the pepper accession, CV3, and identified the other single recessive resistance locus, cvr4, in the pepper accession, CV9. To fine map the Cvr1 gene, we narrowed down the Cvr1 locus to 0 cm using two previously reported markers and pepper genome sequence. Due to the highly repetitive nature of this locus containing nucleotide binding leucine rich repeat (NB-LRR) sequences, we are trying to use another approach, Cas9-Assisted Targeting of Chromosome segements (CATCH) cloning, to fine map the Cvr1 gene. On the other hand, we used bulked segregant analysis RNA sequencing (BSA RNA-seq) approach to map the other ChiVMV resistance gene, cvr4. We identified that the cvr4 was located on the upper region on pepper chromosome 11. Based on RNA-seq data, we could map the cvr4 gene and developed several cvr4-linked markers. We are fine mapping the cvr4 locus with RNA-seq data combining three published pepper reference genome sets at present. This study could help not only the breeding of ChiVMV resistance cultivar and but also the genetic study of disease resistance in pepper. *Corresponding Author: Tel. 02) , bk54@snu.ac.kr 92

149 PB-09 Dongryung Lee, Yoon Kyung Lee, Taejun Kim, Eunbyeol Koh, Hee-Jong Koh * Lesion mimic mutants (LMMs) commonly exhibit spontaneous cell death similar to the hypersensitive defense response that occurs in plants in response to pathogen infection. Several lesion mimic mutants have been isolated and characterized, but their molecular mechanisms remain largely unknown. Here, a spotted leaf sheath (sles) mutant derived from japonica cultivar Koshihikari is described. The sles phenotype differed from that of other LMMs in that lesion mimic spots were observed on the leaf sheath rather than on leaves. The sles mutant displayed early senescence, as shown, by color loss in the mesophyll cells, a decrease in chlorophyll content, and upregulation of chlorophyll degradation-related and senescence-associated genes. ROS content was also elevated, corresponding to increased expression of genes encoding ROS-generating enzymes. Pathogenesis-related genes were also activated and showed improved resistance to pathogen infection on the leaf sheath. Genetic analysis revealed that the mutant phenotype was controlled by a single recessive nuclear gene. Genetic mapping and sequence analysis showed that a single nucleotide substitution in the sixth exon of LOC_Os07g25680 was responsible for the sles mutant phenotype and this was confirmed by T-DNA insertion line. Taken together, our results revealed that SLES was associated with the formation of lesion mimic spots on the leaf sheath resulting early senescence and defense responses. Further examination of SLES will facilitate a better understanding of the molecular mechanisms involved in ROS homeostasis and may also provide opportunities to improve pathogen resistance in rice. *Corresponding Author: Tel , heejkoh@snu.ac.kr PB-10 Won-Ki Hong, Na-Rae Jeong, Heejin Kim, Jin-Ho Kang * Trichomes are hair-like structures derived from the epidermis of plants and specialized epidermal structures that function in the plant defense against biotic and abiotic stresses. Trichomes exist in the wide range of plant species and are classified as either glandular or non-glandular types. The glandular trichomes function in a chemical defense against herbivory, and the non-glandular trichomes function as physiological barriers for biotic and environmental stresses. Trichomes of tomato are classified as being four glandular types (type I, IV, VI, VII) and three non-glandular types (type II, III, V). We describe the characterization of a monogenic recessive tomato mutant (Solanum lycopersicum) called no trichome (nt). To analyze the morphology of trichomes on the nt mutant in detail, we observed with a dissecting microscope and a cryo-sem. The results showed that the nt mutant has normal types of trichomes in all tissues except on young stems compared with wild-type plants. The nt mutant does not have any trichomes in young stems compared to wild-type plants. We are currently doing a map-based cloning to identify NT gene. *Corresponding Author: Tel , kangjinho@snu.ac.kr 93

150 PB-11 Beom-Kyu Kang *, Jeong-Hyun Seo, Hyun-Tae Kim, Hong-Sik Kim, Sang-Ouk Shin, Chang-Hwan Park, Do-Yeon Kwak Soybean seed appearance is a quite important factor for determining commercial value. An irregular cracking in soybean seeds is frequently induced in early mature soybean cultivars and reduce a seed quality. This study was conducted to identified QTL for an irregular cracking in soybean seed coat. 167 F 7 RILs crossed between soybean cultivar Uram, late mature and hard to appear crack and Chamol, early mature and easy to appear crack was used for QTL analysis. Phenotypes were evaluated in Daegu experiment station in 2016 and Genetic linkage map was constructed with Axiom 180K SNP array using IciMapping 4.1. The cracked seeds was counted with three replications in randomly collected 100 seeds from bulked harvested lines. The cracked seeds number of Uram and Chamol in combined year was 1.1 and 28.8, respectively. The mean of RILs was 12.9 and the range was from 0.3 to In correlation analysis, SCC was negatively correlated with plant height, first pod height, yield, flowering day, growing day, but positively correlated with 100-seed weight. The frequency distribution was analyzed after converting cracked seeds number to score from 1(hard) to 5(easy) and close to bimodal distribution which meant that SCC was controlled by one or two major regions. In two environments, a total of 7 QTLs were identified. Among them, qscc2 located on chromosome 2 showed positive additive effects explaining 15.04% of phenotypic variances with 12.9 of LOD score in combined environments. Although the gap of position of qscc2 existed depends on experimental years, it was consistently identifed in two years and combined year. This results showed that an irregular cracking in soybean seed coats in RIL population used in this study was highly related with QTL on chromosome 2. *Corresponding Author: Tel , hellobk01@korea.kr PB-12 Jae-In Chun 1*, Heejin Kim 1, In-Taek Hwang 1,2, Na-Rae Jeong 1, Won-Ki Hong 1, Jin-Ho Kang 1,2 1 2 Trichomes are fine outgrowths derived from epidermal cells on the aerial part of plants. They serve important functions in physical and chemical defense against biotic and abiotic stresses. Trichomes vary in morphology as much as they vary in function. Cultivated tomato (Solanum lycopersicum) has seven types of multicellular trichomes that are either non-glandular or glandular. Glandular trichomes synthesize and secret various metabolites, while non-glandular trichomes act as a physical barrier against herbivores and unfavorable environmental conditions. Despite the important roles of trichomes in plant defense, developmental processes of multicellular trichomes are poorly understood. To identify genes involved in trichome development, Micro-tom mutant population generated by EMS (Ethylmethane sulfonate) mutagenesis was screened. Four mutant lines showing abnormal trichome structure were screened. The mutant lines had distorted and twisted trichomes to a greater or lesser extent compared to wild-type plants. The distorted structure was observed from all the seven types of trichomes on entire plants. To identify genes affecting trichome structure in the mutants, map-based cloning will be performed using a mapping population obtained by crossing the mutant lines with Solanum pennellii. *Corresponding Author: Tel , janechun@snu.ac.kr 94

151 PB-13 Thanh-Tam Ho, Keon-Il Kim, So-Young Park * Nitric oxide (NO) is one of the important signaling molecules that activate defense responses in a plant, and it result in the accumulation of secondary metabolites. In the present study, we investigated the role of NO on ginsenoside biosynthesis from Panax ginseng adventitious root cultures after various concentrations and exposure times to three Lactic acid bacteria (LAB) [Lactobacillus rhamnosus GG (Lr), Lactobacillus sanfranciscensis (Ls), Leuconostoc citreum (Lc)] treatments. The changes of NO were analyzed using a nitric oxide colorimetric assay kit, and the individual ginsenosides were identified by HPLC after LAB treatments. NO was generated immediately after LAB treatments and reached the highest level at 24 h in almost treatment (Ls 0.02% and Lr 0.2% were maximum at 48 h). The highest of NO content (0.996 µmol mg -1 FW) was obtained in Lc 0.2% treatment at 24 h. Correlated with NO generation, total saponin content was enhanced in all treatments compared with the control after 7 days of treatments, especially in Lr 0.2% treatment (35.2 mg g -1 DW); whereas, biomass (FW and DW) production has no significant difference among treatments. The total of ginsenosides was increased after LAB treatments, and the highest of total ginsenosides content accumulation was obtained in Ls 0.2% treatment. This study suggested that NO play as a signal molecule after LAB treatment and it lead to ginsenosides accumulation in the adventitious root cultures of P. ginseng. Keywords: Adventitious root, ginsenosides, lactic acid bacteria, nitric oxide, Panax ginseng. *Corresponding Author: Tel , soypark7@cbnu.ac.kr PB-14 서민정 1*, 김율호 2, 강인정 3, 이영훈 4, 이영기 5, 박명렬 1, 윤홍태 콩은우리나라의전통식생활문화에서단백질을공급하는중요한식량작물로서영양학적가치뿐만아니라지력유지및증진, 논에서벼대체작물활용등의측면에서중요한작물로재배되고있다. 그런데우리나라는콩의발생기원지에속하여유전적다양성이높은만큼발생하는병의종류도다양하여기후변화에따라다양한병의돌발적인발생위험이내재되어있다. 그가운데콩들불병 (Pseudomonas amygdali pv. tabaci) 은불마름병과함께우리나라에서콩의생육후기에발생하는대표적인세균병으로수량감소를초래하는것으로알려져있는데약제방제의효과가미미하여저항성품종을재배하는것이피해를줄이는가장효과적인방법이다. 이러한이유로최근콩들불병저항성계통육성체계를확립하고자연구를진행하고있는데, 다양한변이체들을포함하는콩들불병병원균자체에대한정보가부족한상황이다. 본연구에서는들불병수집균주들을대상으로 GBS(genotyping by seqeucing) 분석을실시하였고이데이터를기반으로실험의편의성을고려하여아가로즈젤기반에서분석이가능한분자마커 (Insertion-deletion; Indel) 를개발하였다. 개발된분자마커는 14 세트이며 PCR 을수행하고제한효소 (SmaⅠ) 로절단하여그사이즈와조각수에대한정보를이용할수있다 (Cleaved amplefied polymorphic seqeunces; CAPs). 국내외에서수집된 45 점의콩들불병균주들을대상으로, 개발된분자마커를적용하여다형성을분석한결과, 균주들의다양성에대한동일한정보 ( 동일한패턴 ) 를제공하는분자마커를제외하고 11 종의분자마커세트를사용하는것이효율적임을확인하였다. 들불병균주들의특성을확인할수있는방법은여러가지가있겠으나본연구에서개발된분자마커를활용하면간편하고신속하게유전적다양성을확인할수있어, 신규로수집되는균주들의다양성및발생균주들의모니터링에도활용할수있을것으로기대된다. 사사 : 본연구는농촌진흥청연구사업 ( 과제번호 : PJ ) 의지원에의해수행되었다. * 교신저자 : Tel , mjseo77@korea.kr 95

152 PB-15 Won-Hee Kang 1, Boseul Park 2, Seon-In Yeom 1,2* 1 2 Receptor-like proteins (RLPs) are well-known to have crucial roles in plant development and defense response against pathogens. But a few RLPs in tomato (Solanum lycopersicum L.) have been functionally characterized though 176 genes encoding RLPs, which have been identified in the tomato genome. To further predict the possible role of tomato RLPs, we performed genome-widely classification and transcriptome analysis. Phylogenetic comparisons revealed that the tomato RLPs were divided into eight subgroups and evolved independently compared to those in Arabidopsis. The localization and physical clustering analyses showed that tomato RLPs were expanded primarily through tandem duplication events. Through analyses of tissue specific RNA-seq data, 71 RLPs were expressed in at least one of the following tissues: root, leaf, bud, flower, or fruit, of which several showed tissue specific expression. In addition, tomato RLP expression profiles after infection with different pathogens showed distinguishable gene regulations to disease induction and resistance response by bacteria and virus. Notably, Some RLPs were highly and/or unique expressed in susceptible tomato to pathogen, suggesting that the RLP could be involved in disease response, possibly as a host-susceptibility factor. Our study could provide an important clues for further investigations into the function of tomato RLPs involved in developmental and response to pathogens. *Corresponding Author: Tel , sunin78@gnu.ac.kr PB-16 Won-Hee Kang 1, Namjin Koo 2, Yong-Min Kim 2, Junesung Lee 1, Seungill Kim 3, Doil Choi 3, and Seon-In Yeom 1* Receptor-like proteins (RLPs) are cell surface receptors, and it is well known to be involved in plant development and defense. To study the characterization of RLPs in pepper, we identified and analyzed RLPs using pepper genome and transcriptome. A total of 438 RLP genes were identified in the pepper genome. CaRLPs were divided into 11 subgroups showing differential expansion. Phylogenetic comparison with Arabidopsis RLPs revealed that pepper and Arabidopsis RLP families evolved independently. Furthermore, transcriptome of RLP genes showed dynamics of global gene expression changes during either plant growth stages or pepper-pathogen interactions. Through transcriptomic data of biotic treatments, we constructed multi-dimensional co-expression network for predicting RLP gene functions by functional modules. Several pathogen-responsive regulatory module of CaRLPs were identified through integrating the co-exporession network and function enrichment tool.s, which cover variable function such as immune responses, metabolism, transcriptional regulation. We expected that the results would be useful keys for functional analysis of RLPs in pepper. *Corresponding Author: Tel , sunin78@gnu.ac.kr 96

153 PB-17 Nayoung Kim, Won-Hee Kang, Hakgi Jang, Kwanbeen Park, Hyunju Ha, Seon-In Yeom * Phytophthora capsici (P. capsici) is one of the destructive soil-borne oomycete in Capsicum spp. To date, Resistance against P. capsici in pepper is controlled by numerous quantitative trait loci (QTLs). The majority of the QTLs have been identified on chromosome 5. For this major QTL. Several molecular markers have been developed but they are still limit to search for P. capsici resistance pepper germplasm and further characterization of resistance gene. In this study, we attain QTL region (6.2Mbp 139.2Mbp) using integrated genetic and genomic data with previously developed molecular markers located on chromosome 5. After then, we re-analyzed domain structure of genes to select candidate resistance gene analogs (RGAs) which has 19 RGAs on extended QTL region. These RGAs are divided into 15 NBS-LRRs (nucleotide binding site leucine rich repeats), 3 RLKs (receptor like kinases) and 1 RLPs (receptor like proteins). To mining single nucleotide polymorphism between P. capsici resistance and susceptible germplasm, we performed multiple sequence alignment and then we developed 11 RGAs based markers. We confirmed that the 11 molecular markers are closely linked to the major QTL for P. capsici resistance through the high resolution melting analysis. Among the 11 molecular markers, M6 is verified as the highest co-segregation marker against P. capsici (86.7%). These could be helpful to characterization of resistance gene against P. capsici and investigation of the P. capsici resistance germplasm. *Corresponding Author: Tel , sunin78@gnu.ac.kr PB-18 박현수 1*, 남정권 1, 김우재 1, 백만기 1, 조영찬 1, 김보경 1, 김기영 2, 신운철 1, 김정주 1, 고재권 1, 이점호 1, 정지웅 1, 강경호 1, 김춘송 1, 정종민 1, 이건미 1, 박슬기 1, 이동규 아이에스 592 비비 (IS592BB) 는다른생태형에비해내병성이약한조생종벼의내병성을증진하기위해개발되었다. 2008/ 09 년동계에최고품질다수성조생품종인 운광 을모본으로하고 운광 과벼흰잎마름병및줄무늬잎마름병저항성유전자를보유하고있는 SR 계통을교배한 F 1 을부본으로삼원교배하여교배번호 HR28420 을부여하였다 년부터 2013 년까지계통육종법을수행하면서병원성이강한벼흰잎마름병 K3a 균계에대한생물검정과벼흰잎마름병과줄무늬잎마름병에대한저항성유전자도입확인을위한 DNA 분자표지선발을통해저항성유전자가집적된계통을선발해나갔다. 조생종으로내병성이증진되었으며농업형질이양호한계통을선발하여 년생산력검정시험을수행하였다. 생산력검정시험에서 조평 보다출수가 2-3 일늦은조생종이며벼흰잎마름병저항성유전자 Xa3+Xa21 과줄무늬잎마름병저항성유전자 Stv-b i 이집적되어있어벼흰마름병과줄무늬잎마름병에대한저항성이향상된우량계통 HR 을선발하여 익산 592 호 라계통명을부여하였다. 익산 592 호 는 년 3 년간실시된지역적응성검정시험결과조생종으로단간내도복다수성특성을가지고있으면서도열병저항성과함께벼흰잎마름병과줄무늬잎마름병에대한내병성이증진되었다는점이인정되어 2017 년 12 월직무육성신품종선정심의위원회에서조생종의내병성증진을위한중간모본으로선정되었고 아이에스 592 비비 (IS592BB) 로품종명을명명하였다. 아이에스 592 비비 (IS592BB) 는복합내병다수성중간모본으로조생종의내병성증진을위한육종소재로서조생종벼육종사업에다양하게활용되고있다. * 교신저자 : Tel , mayoe@korea.kr 97

154 PB-19 문지혜 1*, 허윤찬 2, 박동금 3, 박태성 1,4, 양은영 1, 김수 1,5, 채수영 1, 멜론덩굴쪼김병과검은점뿌리썩음병에저항성인유전자원을계통선발하여 PI 을모본으로하고검은점뿌리썩음병저항성유전자원 PI 을부본으로교배하여덩굴쪼김병과검은점뿌리썩음병에저항성인대목용멜론 뚝심 을육성하였다. 뚝심은덩굴쪼김병과흰가루병에저항성이며, 검은점뿌리썩음병에중간정도저항성을타나낸다. 유묘의배축길이는대조품종과비교하여길며, 떡잎크기는큰편이다. 잎자루길이는길고입자루자세는반직립이다. 과형은난형이고과피색은녹색을띄다가성숙할수록녹황색으로되며과피에점이있고점의밀도는높았다. 과육색은황색빛백색이고당도는 11 Brix 정도로대비종보다낮았다 년여름재배와 2017 년봄재배에서뚝심을대목으로이용하였을때멜론의수량및품질이무접목재배와비교하여비슷한수준이었다. 멜론재배시토양병해발생으로생산성이감소되고있는지역에뚝심을대목으로이용하여접목재배시안정적인생산이가능할것이다. * 교신저자 : Tel , jhmoon@korea.kr PB-20 Suk-Man Kim 1,2*, Rusell Reinke Bacterial blight (BB) disease by the Xanthomonas oryzae pv. oryzae (Xoo) is one of most severe factor regarding rice yield loss in most of rice cultivation countries especially in Asia. Deployment of cultivar with the resistance gene to BB is known as the most effective way to control the disease. However the evolution of new Xoo or pathotypes changed by single gene dependent abuse often result in breakdown of the resistance. Thus, efforts for searching new novel R-gene with sustainable BB resistance has been constantly required. In this study we have identified three QTLs on chromosomes 1, 4, and 11, respectively, with 493 F 2 individuals from across between P6 and Ilpum using 7K SNP-chip. Of theses one major QTL qbb_11 on chromosome 11 explained % of the total phenotypic variance in the population, with an LOD value of harboring SNPs and The single major R-gene which is recessive to BB was designated as xa44(t) and was narrowed down to 470 kb segment franked within 27.98Mbp to 28.45Mbp. The results may suggested useful information to understand BB resistance mechanism and provide DNA marker for MAS breeding to improve BB resistance in rice. *Corresponding Author: Tel , s.kim@irri.org 98

155 PB-21 Jiheun Noh 1*, Gi Hoon Kim 1, Jeong-Hee Kim 2, Dong-Hyuk Lee 2, Yun Su Do 3 and Cheol Choi Apples are important agricultural crops in worldwide. Apple Marssonina blotch that is usually called Apple Blotch disease is one of major apple diseases known in Korea and is caused by Diplocarpon mali.apple Marssonina blotch occurs on leaves and fruits, which lowers fruit quality and makes early defoliation of leaves leads to lower tree growth. Fuji and Hongro are main apple cultivars in Korea and these are known as susceptible to apple Marssonina blotch. But relative genome study has been rare. Genome-wide association study (GWAS) is useful for searching for related genes to the target trait. This study was carry out to detect candidate genes affection resistance to apple Marssonina blotch establish basic data for genomic study in future. From May, large quantity of conidia was produced, we monitored severity of infection on leaf of 730 apple germplasm until October and scored on a six scale follow to Agricultural science technology research analysis standard reference (RDA). Results of pathogenicity showed 1.9% Immune(I) and 4.2% resistant(r), 23.0% Moderately resistant(mr), 28.1% Moderately susceptible(ms), 26.0% Susceptible(S), 16.7% Highly susceptible(hs), respecively. SNPs of 187 core collection are called by using Genotyping-by-Sequencing (GBS) and are filtered thru TASSEL-GBS pipeline with apple reference genome. We performed GWAS analysis with SNPs data. *Corresponding Author: Tel , cc31@knu.ac.kr PB-22 강주원, 이지윤, 조준현, 권영호, 조수민, 박동수, 송유천, 이종희 * 고품질벼인운광벼의단점을개선하기위해줄무늬잎마름병에저항성을보이는해담쌀을 2 회여교배하여선발된 YR32548 에다시해담쌀을교배하여 BC 2F 1 24 개체를얻었다. BC 1F 1 및 BC 2F 1 식물체에서 InDel 7 마커로유전자형을검정한결과이형접합체가각각 12, 11 개체이었으며, X 2 -test 결과 1 개의유전자검정교배시이론적인분리비 1 : 1 에적합한것으로나타났다. 즉, 1 개의우성유전자로밝혀진줄무늬잎마름병저항성유전자가인디카품종 Modan 에서유래되었지만, 인디카 / 자포니카원연교잡에서발생할수있는연관불균형 (linkage equilibrium) 또는분리변형 (segregation distortion) 은발생하지않는것으로판단되어진다. 선발된 11 개체에대하여 background selection 을위한유전자지도작성을위해 KASP Marker 315 개를이용하여다형성검정을실시한결과 23.2% 인 73 개의마커에서양친간의다형성이검정되었고, 이를이용하여 11 개개체에대한유전자형검정을실시하였다. 선발된계통은평균 6.6 개의이형접합체와 1.3 개의해담쌀의염색체단편이이입되어있었으며, 평균단편이입률은이형접합체포함 20% 로 80% 이상운광벼로회복되었음을확인할수있었다. 육종에서의 MAB 의이용은목표형질의개량에있어목표형질을소실시키지않고, 시간과비용을절감할수있다는점에서그효율성이증명되고있다. 선발된개체는 2018 년하계에운광벼, 해담쌀과농업적특성을비교할예정이며, 애멸구접종후줄무늬잎마름병생물검정을실시할예정이다. * 교신저자 : Tel , ccriljh@korea.kr 99

156 PB-23 Hyun-Ju Kang, Jun Oh, Hyo Ja Oh, Song Lim Kim, Inchan Choi, Jeongho Baek, Kyung-Hwan Kim, Hyeonso Ji * Bakanae disease has become a serious threat in almost all rice cultivation regions worldwide. The incidence of bakanae disease is increasing with temperature rise due to global warming, and the advent of fungicide-resistant strains has rendered disease control difficult. We performed bioassay of bakanae disease resistance with 47 rice varieties by in vitro seedling screening method. Samgwangbyo showed the highest resistance with Nampyeongbyeo. We crossed Samgwangbyeo with a susceptible variety, Junambyeo, and developed an F2 population. A genetic map comprising 132 KASP markers which are polymorphic between the parents was constructed with 188 F2 plants. The total distance of the genetic map was 1,863.3 cm, and the average distance between markers was cm. Bioassay of 188 F3 families derived from the F2 plants was also performed by in vitro seedling screening method. The mortality rate of the F3 families ranged from 0 to 100 %, while that of Samgwangbyeo was 1.7% and that of Junambyeo was 83.3%. These data will be utilized in mapping QTLs of bakanae resistance in Samgwangbyeo, which may contribute to breeding bakanae disease resistant rice varieties. *Corresponding Author: Tel , jhs77@korea.kr PB-24 Kieu Thi Xuan Vo, Chi-Yeol Kim, Dae-woo Lee, Seok-Hyun Choi, Suhyeon Shim, Jong-Seong Jeon Numbers of studies last twenty years have emphasized the unordinary role of WRKY family in plants, remarkable in retroactions to environmental stimuli. Among a hundred of WRKY genes in rice, at least twelve were determined to regulate defense response either in positively or negatively fashion. Here we show that OsWRKY67 emerges as a member contributing in resistance to various pathogens in rice. The activation of OsWRKY67 gained by T-DNA tagging significantly improved resistance against Magnaporthe oryzae and Xanthomonas oryzae pv. oryzae (Xoo) whereas OsWRKY67 RNAi lines significantly reduced resistance. OsWRKY67 was then functional deciphered in XA21-mediated resistance as a negative regulator, characterized by the abolishment of XA21-mediated resistance in OsWRKY67 RNAi. Overexpression of OsWRKY67 in rice confirmed enhanced disease resistance, but revealed a restriction of plant growth with high levels of OsWRK67 protein. Quantitative PCR reveals numbers of PR genes are strikingly up-regulated if OsWRKY67 is enhanced meanwhile our transcriptional activity assay and localization that OsWRKY67 is a transcriptional activator. Accordingly, our study offers an additional WRKY candidate into transcription factor category for crop genetic improvement. *Corresponding Author: jjeon@khu.ac.kr 100

157 PB-25 Min Ju Kim 1, Seung Hee Han 1, Kyungmun Kim 1, Sang Hyeon Kim 1 and Si Hyeock Lee 1,2* 1 2 Frankliniella occidentalis is one of the polyphagous pest damaging flowers and leaves of horticultural and agricultural crops. Due to its wide host range and rapid development of insecticide resistance, F. occidentalis is extremely hard to control by conventional insecticides, thus requiring an alternative control measure. RNA interference (RNAi)-based control strategy has been developed to control various phytophagous chewing pests. However, no successful case of RNAi-based control has been reported for sucking pests including thrips. In this study, as the basic information for the establishment of ingestion RNAi against thrips, feeding amount and time course of plant subcellular fractions by F. occidentalis were determined by quantitative PCR (qpcr). F. occidentalis adults were starved for 24 h and then fed with kidney been leaf for 48 h. Thrips were then collected every 6-h interval, their genomic DNA was extracted and the ingested fractions of chloroplast and nuclear were quantified using rubisco and 5S rrna genes as markers, respectively. The ingested amount of rubisco and 5s rrna genes increased rapidly until 6 h after feeding and then slightly reduced over time. Detection of both genes confirms that F. occidentalis ingests both chloroplasts and nucleus along with cytosol during sucking. Relative ratio between two marker genes detected in thrips (1 : 24) was different from that of kidney bean tissue itself (1 : 8000), suggesting that thrips ingested nucleus along with cytosol more selectively compared to chloroplasts. Finally, two different feeding systems (sliced bean and leaf-disc) were tested as double-stranded RNA (dsrna) delivery methods, and the ingested amount of test dsrna (pqe30) was quantified using qpcr. *Corresponding Author: Tel , shlee22@snu.ac.kr PB-26 Kyung Hye Kim 1, Hong Min Koh 1, Ji-Min Kim 1, Taeklim Lee 1, Jinho Heo 1, Jiyeong Jung 1, Ju Seok Lee 2, Soon-Chun Jeong 2, Man Soo Choi 3, Jung-Kyung Moon 3, Bo-Keun Ha 4, Namshin Kim 5, Sungtaeg Kang 1* We developed a Korean soybean[glycine max (L.) Merr] Nested-Association Mapping Population(NAM) and conducted a Genome-Wide Association Study(GWAS) with flowering time. To develop the NAM population, Daepung was used as hub parent and 27 soybean varieties was chosen as founder parents, based on their genetic diversity. Total of 2,619 recombinant inbred lines of 27 combinations were produced. The 180K Axiom SoyaSNP array Chip was employed for genotyping of NAM population. We investigated the flowering time in three environments, Cheonan in 2015 and 2016, and Jeonju in The each flowering time in 3 environments were normally distributed, even though some lines showed different flowering time in different environment. First, to identify the flowering time-related genes/qtl, the genetic linkage map was constructed using each RILs, and QTL analysis was performed. Highly saturated genetic map was composited with a total length of 4,407 cm and an average distance of SNP marker was 0.15 cm. A QTL analysis results showed that QTLs from each population was not identical in 23 RILs and 3 environments. To employ more polymorphic SNPs in genetic linkage map construction and QTL identification, GWAS was conducted using 23 RILs. Interestingly, the results showed that identified QTLs were identical in all 3 environments. The mjaor QTLs were identified on chromosomes 6, 10, and 12, and minor QTLs were identified on many other chromosomes. Among them, QTLs on the chromosomes 6, 10 and 19 were in good agreement with previously reported soybean flowering time locus, E locus. Also many other newly identified QTLs were detected. In summary, constructed Korean soybean NAM population and following GWAS analysis showed that GWAS with NAM population is a highly accurate genes/qtl identification method, and has improved capability to gene identification and great potential for noble genes/qtls identification, comparing to traditional QTL identification procedure using single RIL population. *Corresponding Author: Tel , kangst@dankook.ac.kr 101

158 PB-27 고홍민 1, 김경혜 1, 김지민 1, 이택림 1, 허진호 1, 정지영 1, 강성택 1* 1 기후환경변화에따른오존층파괴는식물의생화학적대사기구장해를일으킨다. 이에대비하여자외선 (UV-B) 에저항성을보이는콩유전자원을지속적으로발굴하고, 발굴된저항성유전자를신속하고안정적으로신품종에도입하기위한 UV-B 저항성유전자의근접마커개발및후보유전자확보로금후고자외선환경하에서도안정적인작물생육과수량확보할수있는기반을마련코자본연구를추진하였다. 본연구에서는국내외수집보관중인재배콩및야생콩유전자원 1,500 여점과 UV-B 저항성유전분석을위해감수성자원과저항성자원을인공교배하여만든 F 2 집단으로저항성검정을실시하였다. 검정방법은다수의기연구자가수행한바와같이 UV-B 인공처리를위해 UV lamp 에 diacetate 필름을처리 (UV-C 제거 ) 하여만들었으며, 조사량은선행연구결과콩에서는 10kJ 이상처리시감수성품종과저항성품종간에차이를보이고있어본실험에서도 10kJ/m 2 /day 전후로처리하였다. 국내수집콩유전자원중재배종 1,276 점, 야생종 186 점을대상으로 UV-B 인공조사를통한저항성검정결과익산 10 호등재배종 5 점과야생종 9 점에서저항성반응을보이는자원을확보하였고, 감수성자원인대풍콩과신팔달콩을저항성자원으로발굴된익산 10 호와인공교배하여확보한 F 2 집단에 UV-B 인공조사후피해증상으로유전분석을실시한결과엽형과엽색및전체피해정도등모든형질에서저항성과감수성이 1 : 3 의분리비를보여자외선저항성유전자는단인자이며열성유전함을확인하였다. 본실험결과에서확인한 UV-B 저항성자원과유전분석결과는추후정밀검정을통해관련유전자확보와더불어분자육종방법을활용한 UV 저항성품종개발에유용하게활용될것으로판단된다. * 교신저자 : Tel , kangst@dankook.ac.kr PB-28 김경혜 1, 고홍민 1, 김지민 1, 이택림 1, 허진호 1, 강범규 2, 김성민 3, 한상훈 4, 이주석 5, 강성택 1* 콩에서탈립성은수량감수의직접적인원인이며저항성계통을선발하기위한특성검정도매우번거롭고많은시간이소요되어육종과정에서저항성계통선발에큰어려움이있다. 따라서본연구에서는내탈립콩품종개발에활용가능한분자마커를개발하고이를활용하기위한연구를수행하였다. 본연구에서는활용한콩탈립성연관 SNP Marker 는 Lee 등 (2017) 이 RIL Line 에서개발한 qpdh1-ks 유래 SNP 를활용하였다. SNP probe 의재현성을검증하기위해참올 대원 F 3 56 계통을 3 반복으로하여 Genotyping 후결과를비교하였고, 국내생산 SNP probe 의육종집단에서적용가능성을검증하고자참올 대원, 황금올 대원의초기세대 F 계통과지역적응시험및생산력검증시험계통 52 계통에대해 Oven dry 방법을통한탈립성검정을실시하고이를 genotyping 결과와비교하였다. 본실험에서활용한 SNP genotyping 은국내기술진에의해개발 (SFC) 된 probe 를사용하였고, SNP detection 은 RT-PCR (StepOne Plus) 로진행하였다. 실험결과, F 3 56 계통을이용한마커의재현성검정에서 3 회모두동일한결과를보여이용된 SNP 및 genotype 기술의안정성이확인되었다. 또한분리세대에서실내검정을통한포장검증표현형과 SNP genotype 비교결과, 초기세대 F 계통에서 92.9% 의정확도를보였고, 다양한모부본으로이루어진지역적응성 / 생산력검정용 52 계통에서는 100% 의정확도를나타내었다. 본연구를통해국내에서개발된탈립성연관 SNP marker 및국내개발 Probe 가콩에서내탈립성계통선발에매우높은정확도보여 DNA 마커를활용한내탈립성계통선발의가능성을확인하였다. 금후이러한기술은콩에서분자육종을통한우수한신품종의조기육성및관련기술확산에크게기여할것으로판단된다. * 교신저자 : Tel , kangst@dankook.ac.kr 102

159 PB-29 Hyun A Jang, Kyo-Sang Lee, Ha-Yeon Yoon, May Moe Oo, Tae-Seok Kwak, Mi-Reu Kim, Sang-Keun Oh * Grapes have cultivated worldwide, especially grown for brewing purposes and accounting for about one-third of the world's fruits production. However, grape ripe rot disease caused by Colletotrichum spp. is an economically important disease in grape production. Therefore, we conducted a study to identify the trait associated with grape disease-resistant and performed genome-wide association study(gwas) for pheno/genomics using grape core collections. Firstly, we conducted phenotypic characterization to prove the pathogenicity of the fungal isolates Colletotrichum acutatum and C. gloeosporioides which were inoculated onto healthy grape leaves. Results of pathogenicity test from 844 grape cultivars(obtained from RDA Korea) showed 726(87%) susceptible and 118(13%) resistance, respectively. Secondly, we selected 350 cultivars (118 resistance and 232 susceptible cultivars) and constructed the Genotyping-By-Sequencing(GBS) library with 96 barcode sets to find the disease-resistant related(nb-lrr) genes. After that, the experiment was carried out to select the candidate genes, phenotype data converted into analyzed format data and performed GWAS analysis with GBS data(using TASSEL software). As a results of GWAS, we identified 6 resistance-related candidate genes for C. gloeosporioides and 7 candidate genes for C. acutatum using filtered 77,126 SNPs by their trait. Among them, only 2 candidate genes were included disease resistance LRR family protein. However, it is necessary to confirm the current results which are actually related or not to resistance genes. Therefore, additional studies should be carried out to confirm resistance genes which are related to current candidate genes. *Corresponding Author: Tel , sangkeun@cnu.ac.kr PB-30 Dae Yoen Kim 1, Min Jeong Hong 2, Yong Weon Seo 1* 1 2 F-box gene family, as one of the largest gene families in plants, plays crucial roles in regulating plant development, reproduction, cellular protein degradation and responses to biotic and abiotic stresses. However, comprehensive analysis of the F-box gene family in wheat (Triticum aestivum L.) has not been analyzed yet. We identified a total of 1,796 F-box genes in wheat genome and these genes were further divided into various subgroups based on specific domains, such as FBA, FBD, DUF, Kelch, Tub, PP2 Arm, cupin_8 Actin, LysM, Myb-binding, pro-isomerase and WD40 domains. In addition, The F-box genes were physically mapped on the 42 wheat chromosomes and duplication events were investigated. F-box genes exhibited functional specificity based on GO analysis, and 47 % of the F-box genes were significantly enriched in ubiquitin-like protein transferase activity (GO: ). Transcriptome and digital expression analysis revealed differential expression patterns of F-box genes which were specifically expressed in various developmental stages and tissues. The genome-wide analysis of F-box genes provides new opportunities for characterization of candidate F-box genes and elucidation of biological roles in growth and development in wheat. Acknowledgement: This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (2016R1C1B ) *Corresponding Author: Tel , seoag@korea.ac.kr 103

160 PB-31 Hye Seon Kim, Kyong Sil Lee, Eun Hee Kim, Duk-Ju Hwang, Il-Pyung Ahn, Shin-Chul Bae, Sang Ryeol Park * We isolated NAC transcription factor related to disease resistance of bacterial and fungal pathogens, Xanthomonas oryzae pv. oryzae (Xoo), Magnaporthe grisea and Fusarium fujikuroi. NAC (NAM, ATAF, and CUC) transcription factors are plant specific gene family and they are involved in plant growth, development, and stress tolerance. This transcription factor family has five groups (I V). On the basis of phylogenetic analysis, isolated and selected OsNAC genes (OsRXI58, OsRXI69, and OsRXI85) fell into group II, III and IV, respectively. To investigate their biological function in the rice, we constructed vector for overexpression in rice, and then generated transgenic rice, respectively. NAC gene expression of overexpressed transgenic rice lines were analyzed by northern blot or RT-PCR, respectively. Analysis of disease resistance to the bacterial leaf blight, blast, and bakanae disease pathogen, OsNAC-overexpressed transgenic rice lines showing high expression level of OsNAC gene were shown more resistant than wild type. These results suggest that OsNAC genes may play regulatory role during bacterial and fungal pathogen infection. *Corresponding Author: Tel , srpark@korea.kr 104

161 PB-33 So-Yeon Park 1, Su-Hyang Song 1, Heng Wang 1, Da-Eun Im 1, San Mar Lar 1, Seong-Gyu Jang 1, Sang-Heon Jin 1, Ja-Hong Lee 1, Jun-Myeong Lee 1, Dong-yong Kim 2, Soon-Wook Kwon 1* 1 2 In domestic, Korean Cymbidium(Cymbidium goeringii) is belonging to Orchidaceae Cymbidium, perennial plant. Distinguishing the Korean cultivar from other national cultivars is difficult. Therefore, we selected 23 In/Del regions that have difference more than 10bp sequence among 370 Indel region which is identified between Korean cymbidium and China cymbidium (NC_028524) for development of cultivar distinguishing system. To develop Korean cymbidium specific markers, We detected adaptable marker by using the 12 Korean cymbidium and 12 Chinese cymbidium for develop specific marker through using selective region. Inel showed a moderately PIC value (0.24) in Korea cymbidium, Indel showed a high PIC value (0.58) in Chinese cymbidium. Especially, Indel 1520 didn t have polymorphism because of appeared only 205bp band in Korea cymbidium, it showed high PIC value (0.45) and didn t appeared 205bp band in Chinese cymbidium. When InDel merker was used with SSR marker, it would be powerful for distinguishing more than use only one marker. This study was performed by support of IPET ( ). *Corresponding Author: Tel , swkwon@pusan.ac.kr PC-01 Soo In Lee *, Jin A Kim, Mi-Jeong Jeong Serine/arginine-rich (SR) gene family members can diversify the transcriptome and proteome of eukaryotes by facilitating the alternative splicing (AS) of precursor messenger RNAs. Herein, we investigated the evolutionary dynamics, AS patterns, and expression levels of the Brassica rapasr (BrSR) gene family in young seedlings treated with abiotic stresses.a comparative genomic analysis identified 25 BrSR genes at 18 Arabidopsis loci and three BrSR-like genes at two Arabidopsis loci. Thirteen of these loci were singletons, while seven loci carried paralogs. All the duplicated pairs were determined to be under purifying selection pressure. The expansion of the BrSR gene family was found to be the result of segmental duplications only. Additionally, the expression levels of 78.6 % (22 of 28) and the AS patterns of 60.7 % (17 of 28) of the BrSR genes were altered in response to abiotic stresses. Among the analyzed abiotic stresses, oxidative, cold, and heat treatments induced the largest expression changes, while cold and heat stresses caused most AS events. Our findings provide insights into the evolutionary dynamics of BrSR genes following polyploidization events, and provide an important resource for future studies aimed at characterizing the specific function(s) of BrSR genes in plant growth, development, and defense. *Corresponding Author: Tel , silee@korea.kr 105

162 PC-02 Hyeong Cheol Park 1*, Jung Eun Hwang 1, Yun Jeong Kim 1, Myung Hwan Shin 1, and Woo Sik Chung Cadmium (Cd) is one of the most toxic heavy metals and a non-essential element to all organisms, including plants; however, the genes involved in Cd resistance in plants remain poorly characterized. To identify Cd resistance genes in rice, we screened a rice cdna expression library treated with CdCl 2 using a yeast (Saccharomyces cerevisiae) mutant ycf1 strain (DTY167) and isolated two rice phytochelatin synthases (OsPCS5 and OsPCS15). The genes were strongly induced by Cd treatment and conferred increased resistance to Cd when expressed in the ycf1 mutant strain. In addition, the Cd concentration was 2-fold higher in yeast expressing OsPCS5 and OsPCS15 than in vector-transformed yeast, and OsPCS5 and OsPCS15 localized in the cytoplasm. Arabidopsisthaliana plants overexpressing OsPCS5/-15 paradoxically exhibited increased sensitivity to Cd, suggesting that overexpression of OsPCS5/-15 resulted in toxicity due to excess phytochelatin production in A. thaliana plants. These data indicate that OsPCS5 and OsPCS15 are involved in Cd tolerance, which may be related to the relative abundances of phytochelatins synthesized by these phytochelatin synthases. *Corresponding Author: Hyeong Cheol Park, hcpark@nie.re.kr PC-03 Dool Yi Kim 1, Kyung Hwa Kim 1, Man Soo Choi 1, Mi na Jin 1 and Jae Buhm Chun 1 1 The EMS induced mutants has been used for disease resistance, abiotic stress, and quantitative including yielding ability. The two maize inbred lines treated with 0.3%, 0.5%, 0.7%, and 0.9% EMS respectively for 8 h. The results of phenotyping analysis of mutagenized maize population in each treatment condition that we couldn t obtained M 2 generation seeds from condition treated with 0.7% and 0.9% EMS(v/v) for 8 h. otherwise, it is shown variable phenotypic variation in conditions with treated 0.5% EMS(v/v) for 8 h, however we could obtained mutagenized M 2 generation seeds for a salt-tolerant analysis. A total of 1041 independent M2 familiar of EMS-induced maize inbred mutants were investigated for salt tolerance. We selected salt tolerance maize inbred lines from mutants populations treated with 0.7% NaCl for 3 weeks in the greenhouse. A salt tolerant mutants was identified in M2 mutant populations. We generated whole-genome sequencing data to the two maize inbred mutants for genetic variation analyses of salt-tolerant enhanced in mutant populations. we expect to the result which is significantly impacts genetic studies of these maize inbred lines as comparative genomics. *Corresponding Author: Doolyi Kim, Tel , dykim22@korea.kr 106

163 PC-04 김경화 1, 최만수 1, 전재범 1, 진민아 1, 안억근 2, 박향미 2, 김둘이 1* 1 2 염분은작물의수확량을줄이는주요비생물적스트레스중하나로알려져있다. 따라서간척지와같이염농도가높은토양에서사료용벼의재배를위해서는내염성이증진된사료용벼의개발이필요하다. 이러한내염성이증진된사료용벼의개발을위하여목양과 IR64 를교배하여얻은 58 계통에대해우선내염성이우수한계통을선발하기위하여기내배양실험을실시하였다. 기내배양에의한염처리방법은교배종으로사용한목양, IR64, 포카리, FL478 품종을 H 2 O, 0.1, 0.2, 0.3, 0.4% NaCl 농도에서 2 주간재배하여생육상태를분석한결과염농도가 0.3% 이상이되면뿌리생장이거의되지않았다. 또한, 0.3% 배지에서 1 주와 2 주간재배하여염이전혀없는배지에옮겨식물체의회복상태를확인한결과 0.3% NaCl 에서 1 주간재배한후물로옮긴배지에서는거의정상적으로회복이되었으나, 같은조건에서 2 주간재배한것에서는정상적인회복이되지않았다. 위실험조건을바탕으로목양과 IR64 의 58 교배계통을이용하여 0.3% NaCl 배지에서 2 주간재배하여식물생장을관찰한결과 58 계통중대조군인목양과 IR64 보다더잘생육하는 11 계통을선발하였다. 이들선발계통들에대해벼의내염성관련유전자로알려진 Saltol 유전자의 19 개마크를이용하여목양과 IR64 의 Saltol 유전자의삽입여부를 PCR 을이용하여확인하였다. 19 개프라이머들중목양과 IR64 의 Saltol 유전자가명확하게구별되는 6 개의프라이머를이용하여선발된 11 교배계통에대해 Saltol 유전자의삽입타입을결정하였다. PCR 결과 11 계통중 4 계통이목양과 IR64 의 Saltol 유전자가동시에삽입되어있는것을확인하였고, 4 계통은 IR64 유전자가삽입되어고정된타입, 2 계통은목양유전자가삽입되어고정된타입으로확인되었으나 1 계통은프라이머에따라목양과 IR64 Saltol 유전자가각각다르게나타나는결과를보였다. 위실험결과를토대로, 기내배양에의해선발된 11 교배계통들에대해토양에서도유사한내염성결과가나오는지에대해확인중에있다. 또한선발된 11 교배계통들에있어목양또는 IR64 타입의 Saltol 유전자간내염성증진과의상관관계를분석할예정이다. * 교신저자 : Tel , dykim22@korea.kr PC-05 Ryulyi Kang 1, Gyutae Kim 1, Woon Ji Kim 1, Si-Yong Kang 2, Dong-Kwan Kim 3, Bo-Keun Ha 1* Cowpea (Vigna unguiculata L.) is rich in vitamins (B1 and B2), lysine and polyphenols, which are good for muscular growth and development, anticancer and anti-aging in human. In addition, this crop is suitable for cooking with rice, rice cakes, soups and stews. In this study, morphological responses were investigated in cowpea plants with two different types of radiations, the proton-beam and the gamma-ray. Seeds of Okdang cultivar were exposed to 100, 200, 300, 400 and 500 Gy of gamma-ray and proton-beam, respectively. After 5 days of sowing, the germination rate tended to decrease with increasing dose regardless of the type of radiation, but all treatments showed more than 90% germination rate after 10 days of sowing. The survival rate decreased significantly over 300 Gy. The survival rates of proton beam and gamma ray at 500 Gy were 35% and 27%, respectively. The half-lethal dose (LD 50 ) of Okdang was 327 Gy in gamma ray and 330 Gy in proton beam. The plant height and the fresh weight of shoot tended to decrease with increasing dose in both radiations with a significant difference from the control group except 100 Gy of gamma-ray in fresh weight. This study will be valuable as a basic research to compare the mutagenic effects of two different types of radiation in cowpea. *Corresponding Author: Tel , bkha@jnu.ac.kr 107

164 PC-06 Kitae Song 1, Hyochul Kim 1, Kyung-Hee Kim 1, Jun-Cheol Moon 2, Jae Yoon Kim 3, Byung-Moo Lee 1* Maize has high food and industrial value, but has difficulties in research because of their complex and huge size genome. Nested association mapping (NAM) was constructed to better understand maize genetics. However, most studies were conducted using the reference genome B73, and only a few studies were conducted on tropical maize. We analyzed the genetic characteristics of Ki3, one of the NAM parent lines, which are tropical maize for various genetic studies using RNA sequencing and bioinformatics tools. As results, a total of 30,526 genes were expressed, and expression profile were constructed. In addition, high-density polymorphisms including 408,193 single nucleotide polymorphisms (SNPs), 22,367 multiple nucleotide polymorphisms (MNPs) and 83,793 insertions and deletions (InDels) were found compared to reference genome. Among them, 14.2 % of polymorphisms (73,172) were passed non-synonymous test which could alter amino acid sequences. A total of 15,396 expressed genes have non-synonymous polymorphisms. Our results offer resources for development of gene-based marker and will help to genomic studies of maize. Acknowledgement: This work was carried out with the support of Cooperative Research Program for Agriculture Science &Technology Development (Project No. PJ ) Rural Development Administration, Republic of Korea. This research was supported by Administration, Republic of Korea,the Golden Seed Project ( SB710), Ministry of Agriculture, Food and Rural Affairs (MAFRA), Ministry of Oceans and Fisheries (MOF), Rural Development Administration (RDA), and Korea Forest Services (KFS). *Corresponding Author: Tel , bmlee@dongguk.edu PC-07 Hyemin Kim, Hyeona Hwang and Hojin Ryu * A plant steroid hormone, Brassinosteroids (BR), is essentially involved in diverse growth and developmental processes in whole life cycles of plants. The BR related transcription factors, BES1 and BZR1, regulate a range of global gene expressions in response to BR and several external signaling cues. However, the molecular mechanisms of BES1/BZR1-mediated transcriptional reprograming are still unclear. In this study, we elucidate that protein complex formation of BES1/BZR1 with Histone deacetylase19 (HDA19) via evolutionary conserved EAR motif is essential for regulation of BR signaling related gene expressions. Defects in BR related functions of EAR motif mutated BRI1-EMS-SUPPRESSOR 1 (BES1) and BRASSINAZOLE-RESISTANT 1 (BZR1) were completely recovered by artificial fusion of either SRDX, TPL or HDA19 proteins. RNA-seq analysis of bes1-dmear and bes1-dmear-hda19 overexpression plants supported the essential roles of HDA19 activity for BES1/BZR1-mediated BR signaling regulations. In addition to BR related gene expressions, the BES1-HDA19 transcription factor complex is importantly involved in development, defense and abiotic stress related to drought stress tolerances. Our data suggest that activation of BR signaling pathways is integrated to complex formation of BES1/BZR1 with HDA19 for fine-tuning of BR related gene networks in plants. *Corresponding Author: hjryu96@chungbuk.ac.kr 108

165 PC-08 Ji Seon Song 1*, Suyeon Kim 1, Mi Hyun Cho 1, Man-Kee Baek 2, Doolyi Kim 3, Beom Gi Kim 1, and In Sun Yoon Salinity is a major factor limiting crop productivity worldwide. Salinity tolerance is a complex agronomic trait and diverse mechanisms are associated with different aspects of salt stress. Saltol is a major QTL associated with seedling stage salt tolerance, and OsHKT1;5 within the Saltol locus is known as a key gene responsible for maintaining high shoot K + /Na + ratio (SKC1) under the salt stress in rice. Cheongho Byeo is a moderate salt tolerant Korean rice cultivar developed for adaptation to the Gyehwa reclaimed saline land, but the molecular components underlying salt tolerance is not well characterized. In the present study, genotyping analysis of Saltol locus of Cheongho Byeo and other Gyehwa rice varieties was conducted by direct sequencing of OsHKT1;5 gene and PCR with simple sequence repeat (SSR) markers. The OsHKT1;5 alleles found in the Gyehwa rice varieties are different from those of salt tolerant Indica rice, Nona Bokra or FL478. This indicates that SKC1 allele is not incorporated in those Korean rice cultivars. It is noted that SSR marker RM3412 showed polymorphism among rice varieties with different salt tolerance, suggesting that genomic variation of this region may contribute salinity tolerance difference. Differential degree of salt-induced expression of OsHKT1;5 gene was observed among rice varieties. Morpho-physiological evaluation of Cheongho Byeo and confocal Na + imaging analysis in root tissues using CoroNa Green is currently undergoing. Supported by grants (PJ and PJ ) from RDA. *Corresponding Author: anny@@gmail.com PC-09 Mi Hyun Cho *, Suyeon Kim, Ji Seon Song, Beom Gi Kim, and In-Sun Yoon Drought and Salinity are major important factors affecting growth and productivity of rice. Some RING zinc-finger proteins, such as XERICOin Arabidopsis, have been reported to enhance drought and salt tolerance by increasing abscisic acid (ABA) biosynthesis. Here, we reported a newly identified gene encoding a RING zinc-finger protein which involved in drought and salt stress response in rice. From microarray analysis, a salt stress-induced C 3HC 4 type RING finger gene was selected for further analyses and designated OsRF1. To investigate its roles in drought and salt stress response, OsRF1 overexpressing rice (OsRF1-OE) was constructed. Soil-pot assay indicated that OsRF1-OE was more tolerant to drought and salt stress compare to wild-type. In leaf-water loss assay, OsRF1-OE exhibited decreased water loss rate compare to wild-type, suggesting that the drought tolerance trait of OsRF1-OE due to its lower water loss rate. Measurement of endogenous ABA content using TQ LC/MS revealed that the endogenous ABA content of OsRF1-OE was higher than that of wild-type regardless of salt stress. Consistent with this, expression level of some ABA biosynthesis genes and ABA-responsive genes were higher in OsRF1-OE than in wild-type. Further yeast two-hybrid assay indicated that the OsRF1 proteins interact with several PP2C proteins directly. The combined results suggested that OsRF1 confers drought and salt tolerance in rice by inducing ABA signaling via increasing ABA biosynthesis and direct interaction with PP2C proteins. Supported by grants from RDA. *Corresponding Author: chogyver73@daum.net 109

166 PC-10 Jin-Ae Kim, Nikita Bhatnagar, Myung Ki Min, Sujung Ra, In Sun Yoon, Young-Joo Seol, Beom-Gi Kim * Overexpression of ABA receptors has previously been reported to enhance drought tolerance, but also to cause stunted growth and decreased crop yields. Here, we screened the signaling activity of monomeric ABA receptors under various ABA concentrations using a transient expression system in rice. Among the ABA receptors, an OsPYL/RCA had the lowest ABA-responsive signaling activity. Further, transgenic rice overexpressing the OsPYL/RCAR showed neither an ABA-sensitive nor an osmotic stress-tolerant phenotype in plate assay, and had similar stem heights and total seed yields compared to those of the control rice cultivar. In yeast two-hybridization and biomolecular fluorescence complementation experiments, the OsPYL/RCAR required a higher concentration of ABA to interact with protein phosphatases compared to other ABA receptors. Under high-aba treatment conditions, the OsPYL/RCAR was also able to repress phosphatase activity, despite having much lower activity than OsPYL/RCAR5. Notably, transgenic rice overexpressing the OsPYL/RCAR exhibited enhanced drought tolerance, lost less water and showed no effect on growth compared to the wild type. Stress marker genes were induced more than in wild type only under very high concentrations of ABA. We conclude that the OsPYL/RCAR is functional as an ABA receptor only under high concentrations of ABA or harsh stress conditions. Thus, transgenic rice over-expressing OsPYL/RCAR showed drought tolerance, (i.e., harsh stress conditions) but exhibited no defects under normal growth conditions. These results lay the foundation for a new strategy to improve abiotic stress tolerance of crop without yield penalty. *Corresponding Author: Tel , bgkimpeace@korea.kr PC-11 Sujung Ra, Suk-Jun Moon, Jin-Ae Kim, Myung Ki Min, In Sun Yoon, Young-Joo Seol, Beom-Gi Kim * Plants adapt to adverse environments through molecular and physiological responses such as ABA signaling pathway, stomatal regulation or root elongation. Gene expression regulation is also one of major responses of plants against adverse environment. Several transcription factors are identified as master switches to induce the stress tolerant genes. DREBs is one of the important abiotic stress tolerant transcription factors. Those are evolutionary conserved in plants and a subfamily of AP2/ERF superfamily. Rice might have two different kinds of DREB groups, OsDREB1 and OsDREB2 which consists of 11 and 6 members in genome. We tried to characterize an unidentified member of DREB1, OsDREB1H which is induced cold specifically. This gene is induced only in the cold stress condition and expressed in leaf sheath and leaf blade but not in root and flower. Transgenic rice overexpressing this gene presents strongly cold tolerance and growth retardation like other transgenic rice overexpressing OsDREB1 genes. However, the transgenic rice doesn t show drought and salt tolerance. Cold responsive genes were induced much in transgenic rice overexpressing DREB1H. Promoters of those induced genes also can be induced by OsDREB1H in protoplasts. Thus, this OsDREB1H is a typical CBF/DREB1 member functioning specifically in cold stress. This gene can be very useful to develop transgenic rice for cold stress tolerance. *Corresponding Author: Tel , bgkimpeace@korea.kr 110

167 PC-12 So-Young Kim, Min Kang, Hye-min Lim, Seung Uk Ji, Gang-Seob Lee * Plants often face a variety of biotic and abiotic stresses that influence their development, growth and productivity. Salt stress is particularly an important abiotic stress that seriously affects plant growth and development. Transgenic potatoes expressing glyceraldehyde-3-phosphate dehydrogenase (GPD), isolated from the oyster mushroom, Pleurotus sajor-caju, had increased tolerance to salt stress. The over-expression of PsGPD in PsGPD-OX transgenic rice was confirmed by quantitative RT-PCR and western blot analysis. To elucidate the role of PsGPD in stress tolerance, responses of PsGPD-OX transgenic rice plants to salt stress conditions were examined. PsGPD-OX #5, #6, and #17 lines were treated with salt stress on MS medium containing 100 mm or 200 mm of NaCl for 5 and 14 days. Morphological analysis revealed differences between the three transgenic PsGPD-OX rice and the wild-type rice. The germination rates of the three transgenic PsGPD-OX lines of rice were significantly higher than that of the wild type rice, indicating that they were more tolerant to 200 mm NaCl than the wild type rice. In addition, the three transgenic PsGPD-OX rice lines had significantly longer length of root and shoot compared to the wild type rice. These results suggested that overexpression of PsGPD improve more tolerance to salt in rice. *Corresponding Author: Tel , kangslee@korea.kr PC-13 Min Kang, So-Young Kim, A-Ram Kim, Seung Uk Ji, Gang-Seob Lee * Plant is frequently exposed to various abiotic stress. Salt stress is particularly an important abiotic stress that seriously affects plant growth and development. BrTSR53 gene, a putative stress-related gene isolated from Brassica rapa, was used to generate overexpression transgenic rice. The over-expression of BrTSR53 in BrTSR53-OX transgenic rice was confirmed by quantitative RT-PCR and western blot analysis. To elucidate the role of BrTSR53 in stress tolerance, responses of BrTSR53-OX transgenic rice plants to salt stress conditions were examined. BrTSR53-OX #12, #28, and #32 lines were treated with salt stress on MS medium containing 100 mm or 200 mm of NaCl for 5 and 14 days. Morphological analysis revealed differences between the three transgenic BrTSR53-OX rice and the wild-type rice. The germination rates of the three transgenic BrTSR53-OX lines of rice were significantly higher than that of the wild type rice, indicating that they were more tolerant to 200 mm NaCl than the wild type rice. In addition, the three transgenic BrTSR53-OX rice lines had significantly longer length of root and shoot compared to the wild type rice. These results suggest that the BrTSR53 gene played an important role in the tolerance of rice to salt stress. Therefore, it might be a potential target for the purpose of improving salt tolerance of rice and other crops. *Corresponding Author: Tel , kangslee@korea.kr 111

168 PC-14 Jae Yoon Kim 1*, Sang Yong Park 1, Kyeong Do Min 1, Chang-Ho Kim 1 1 Pre-harvest sprouting (PHS) is the precocious germination condition of grains while the spike is still in the mother plant. Because PHS in wheat drastically reduced the quality and economic value of wheat grain, the improving PHS wheat is one of the most important breeding goal in Korean wheat breeding program In this study, we performed comparative expression analyses of genes related to phytohormone biosynthesis and signaling, especially ABA and GA, in two Korean wheat cultivars with different responses to PHS (tolerant/susceptible) using high-throughput RNA-seq technology. In order to perform RNA-seq, Two Korean cultivars of common wheat, Keumgang (Geuru/Kanto75//Eunpa, PHS sensitive, Korea RDA accession no. IT213100) and Woori (Geuru/Ol, PHS resistant, Korea RDA accession no. IT175538) were analyzed using Hi-Seq A total of 123 unigenes were related to the biosynthesis or signaling of ABA, gibberellic acid, indole-3-acetic acid, and cytokinin, and 1862 of differentially expressed genes (DEGs) were identified and categorized into eight groups. The majority of DEGs were involved in sugar-related processes, which interacted with ABA signaling in PHS-induced grains of the PHS-tolerant cultivar. These findings indicate that ABA-related genes are key regulators of dormancy and germination in winter wheat and provide insight into PHS-induced changes in the expression of plant hormone-related genes. Acknowledgement: This work was carried out with the support of "Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ )" Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , jaeyoonkim@kongju.ac.kr PC-15 현웅조 1*, 원용재 1, 홍하철 1, 정국현 1, 안억근 1, 박향미 1, 윤미라 4, 이정희 4, 이상복 2, 신동범 우리나라식량자급률제고를위해중부지역은다양한식량작물이재배되고있으며이러한작물들을효율적으로재배하기위해다양한작부체계가필요하다. 작부체계의성공을위해서는작물재배기간이중첩되는것을피해야하고이를위해벼를일반재배보다빨리재배하는조기재배또는늦게재배하는만기재배에적응할수있는벼품종개발이필수적이다. 또한중부지역에오랜기간재배되고있는외래품종의교체를위해도정특성과쌀품질이우수한품종개발역시필요하다. 중부지역에적응하는밥맛과재배안정성이우수한조생품종개발을목적으로 해들 은고품과강원 4 호를각각모부본으로하여 2007 년에교배되었다. 세대단축을위해 F 1 세대의약을배양하여 AC3 세대에서초형과쌀품질이우수하고도열병및흰잎마름병저항성을보인 SR31523-HB 을선발하여, 수원 588 호의계통명을부여하였다. 2 년간의생산력검정시험과 3 년간의지역적응시험결과그우수성이인정되어 2017 년직무육성신품종심의회에서최고품질벼로선정되었다. 해들 은조생종으로밥맛과재배안정성이우수하여생산자부터소비자까지만족할수있고, 다양한작부체계에적응할수있는품종으로역할이기대된다. 사사 : 본연구는농촌진흥청연구사업 ( 세부과제명 : 중부지역수요자참여형고품질밥쌀용벼품종개발, 세부과제번호 : PJ ) 의지원에의해이루어진것임. * 교신저자 : onlybio@korea.kr 112

169 PC-16 Young Sam Go 2, Tae Ho Ryu 1, Seung Hee Choi 1, and Jin-Hong Kim 1* 1 2 DNA double-strand breaks (DSBs) are toxic lesion that can lead genetic instability and can be occurred by gamma rays and genotoxic stress in plants. To preserve chromosomal integrity from DNA damage such as DSBs, plants have a DNA damage response (DDR) system that regulates programmed cell death (PCD), DNA repair, and cell cycle arrest. However, in plants, it was little unknown about transcription factors are involved in the DDR system. In this study, we confirmed that transcript of DDR-related genes and NAC103 gene was increased in gamma rays- and genotoxic stresses-treated wild type. In addition, the expression of DDR-related genes, such as Rad51, GRG, RPA1E, BRCA1 and PARP1 genes regulated in NAC103-GFP overexpression lines and nac103 mutant, but not changed in NAC103 overexpression lines. Moreover, generation of true leaves and cell death of roots are decreased in sog1 and nac103 mutant compare to wild type under genotoxic stress. Transcriptional activation assay and chromatin immunoprecipitation analysis suggested direct or indirect interaction of NAC103-GFP with promoters of DDR-related genes. Furthermore, expression of DDR-related genes was induced in NAC103-GFP OX lines and NAC103 OX lines of sog1 mutant under genotoxic stress. Taken together, these results demonstrate that NAC103, which is regulated by SOG1, serve as a part of regulator of DDR under DNA damage condition. *Corresponding Author: Tel , jhongkim@kaeri.re.kr PC-17 Dae Gyeom Oh 1* and Cheol Seong Jang 1 1 The general negative effect by global warming is affect on plant survival due to the damaging effect of high temperatures on plant development. However, it is far little known that functions of RING E3 ligase in response to high temperature in plants. To identify genes required for the heat stress response in the cereal crops, we found that the Sorghum bicolor (sorghum) ortholog of Oryza sativa heat and cold induced (OsHCI1), SbHCI1 protein, which is highly induced under high temperature condition in sorghum. Subcellular localization results showed that SbHCI1 was mainly associated with the cytosol and moved to Golgi apparatus when exposed to high temperature conditions. In addition, results of Bimolecular fluorescence complementation (BiFC) and yeast two-hybrid (Y2H) assay showed that SbHCI1 physically interacted with ortholog partner proteins of OsHCI1, i.e. SbbHLH, Sb and SbBGLU1, at cytoplasm within the cell. Moreover, in vitro ubiquitination assay revealed that SbHCI1 ubiquitinates each of three interacting proteins. Therefore, SbHCI1 is induced by heat stress and suggests that it s activation as an e3 ligase. *Corresponding Author: Tel , csjang@kangwon.ac.kr 113

170 PC-18 Hwan Hee Bae *, Sang Gon Kim, Jin-Seok Lee, Jung-Tae Kim, Beom-Young Son, Young Sam Go, Sun-Lim Kim, Seong-Bum Baek Despite its relevance, transcript regulation, protein regulation, and the physiological status of plant under water deficit are not well understood in F1 hybrid maize developed in Korea. In this study, F1 hybrid maize exposed to water deficit conditions by withholding water for 10 days at flowering stage. In both IImichal (Ilmi) and Gwangpyeongok (GPOK), the water deficit stress severely caused to reduce relative leaf water content and decrease leaf area by about 36 and 45%, respectively. Stem length and total dry matter of aerial parts of the water deficit-stressed plants was decreased in Ilmi and GPOK, respectively. Root dry matter accumulation was only reduced by about 25 % in GPOK. SPAD value and leaf conductance of the water deficit stressed plants severely decreased in both hybrids. 2-DE analyses were compared between well-watered and water-deficient F1 hybrid maize. Differentially expression was observed for 24 protein spots due to water deficit stress. Major identified proteins by MALDI-TOF mass spectrometry were involved in carbohydrate metabolism, stress response, and photosynthesis. Out of the 24 differentially expressed proteins, seven stress responsive proteins were highly expressed in both F1 hybrid by water deficit stress compared to well-watered plants. Interestingly, delta 3,5-delta 2,4-dienoyl-CoA isomerase and bifunctional 3-phosphoadenosine 5-phosphosulfate synthetase 2 were only expressed in GPOK. Otherwise, NAD-dependent epimerase/dehydratase, NAD(P)H-quinone oxidoreductase subunit 2 A, and uncharacterized protein were expressed in Ilmi response to water deficit stress. Semi-quantitative RT-PCR analysis showed that mrna expression level of most of genes encoding the identified proteins was well correlated with their protein abundance, suggesting their water deficit-dependent transcriptional regulation in F1 hybrid maize at flowering stage. *Corresponding Author: Tel , miami9@korea.kr PC-19 Yoonjung Lee 1, Mijeong Kim 1, Taegyu Kim 1, Tae-Ho Ham 1 and Joohyun Lee 1* 1 Rice yield is influenced by various factors, Salt is one of crucial factor. In this study, we conducted GWAS analysis with 137 varieties of rice core set to identifying genes which response to salt stress during germination in rice. Seeds were germinated and grown under 250nM NaCl solution for 10 days in a growth chamber with 12, 40% of relative humidity. After 10 days, we measured 137 varieties seeds of germination rate, germination speed, germination energy, germination uniform rate and shoot length. These phenotype data were analyzed by GWAS. As a result, We found 11 SNPs markers related with germination under salt stress. Two candidate genes for germination rate, 2 candidate genes for germination speed, 3 candidates genes for germination uniform rate, 2 candidate gens for germination energy and 2 candidate genes for shoot length were detected by GWAS analysis. *Corresponding Author: Tel , joohyun00@gmail.com 114

171 PC-20 In-Sok Lee 1*, Chan-Ho Kang 1, Jeong-Man Kim 1 1 The main objective of this study was to evaluate the four forage crops at the reclaimed land of Jeollabukdo-Province based on the evaluation of plant height, tiller, fresh weight, dry weight, and K/Na ratio. Four crops such as barley, rye, triticale, and italian ryegrass in this study were cultured at the reclaimed land. During cutivation period, the field was evaluated for moisture (%), electrical condutivity (EC, ds/m), and hardness (kg/cm 2 ) 13 times. The moisture ranged from 5.37 to The EC level was 0.45~1.27. The soil hardness showed 0.45~1.27 value. In vegetative period, a plant height (48.7 cm) of rye was the highest over the others. With regard to tiller number, italian ryegrass showed the greatest tillering capacity with 5.7 on average. But, there was no statistically significant difference. As compared to a dry weight per plant, barely showed the heaviest measurement as 1.64 g, italian ryegrass lowest as 0.38 g. During reproductive growth period, a plant height of rye was much higher than that of other crops. It is similar to the result of vegetative period. A tiller number was same in both barley and italian ryegrass with 3.4 on average. A fresh weight was high in the order of italian ryegrass (10.16 kg/m 2 ) > rye (5.53 kg/m 2 ) > triticale (4.2 kg/m 2 ) > barley (4.1 kg/m 2 ). A average dry weight of rye and Italian ryegrass was 1.8 and 1.81 kg/m 2, respectively, which was significantly high over that of the others. At non-reclaimed land, a K/Na ratio was nearly equal to rye, triticale, and italian ryegrass. But, its level (159.4) of rye was the highest compared to that of the others. A barley showed a heavy imblance of growth in the reclaimed land. The heading stage of barley was the fastest. Based on a K/Na ratio, it could be concluded that the rye is a suitable to produce a coarse forage in the reclaimed land. In the immediate future, the feed value of forage crops will be analyzed. *Corresponding Author: Tel , bioplant325@korea.kr PC-21 김정주 1*, 남정권 2, 정종민 1, 정지웅 1, 고재권 1, 김보경 1, 이점호 벼정조저장중저장조건에따라최고품질벼품종의도정특성변화에미치는영향을평가하기위하여저온 (15 ), 저온밀봉, 상온조건에서저장한벼품종의도정특성, 수분함량, 현미의경도및밥의윤기치등을조사하였다. 저장중밥의윤기치는저장전에비해감소하는경향이었고저온저장보다상온저장에서밥의윤기치감소정도가더심한경향이었다. 저장전시료의수분함량은 12.5 ~ 14.6% 이었고저장중수분함량변화는저온밀봉, 상온, 저온저장순으로감소하는정도가심하였으며현미의경도는저온밀봉, 상온, 저온저장순으로수분함량이감소하는정도에따라높게나타냈다. 저온저장의경우현미의수분함량이낮고현미의경도가높아싸라기발생률이높았으며현미를백미로도정할때의현백률이높게나타났다. 따라서, 백미를색체선별할때색체선별불완전미비율또한높아졌다. 그러나상온및저온밀봉조건에서저장한시료는저장전과비교해도정특성에는큰차이가없었다. 상온저장보다저온저장조건에서싸라기비율및현백률이높아진이유는상온저장에서는대체로정조의수분함량이낮아지는정도가적은반면저온저장에서는상대습도조절이안되는저장고에서찬바람을이용해온도가조절되는조건으로저장 1 개월만에 8~9% 로빠르게수분함량이감소하고현미의경도가높아졌기때문이다. 수분함량이낮은상태에서도정을하면싸라기발생률이높고도정이잘되지않아색체선별과정에서색체미로선별되어완전미도정수율이낮아지는원인이된다. 따라서, 저장시설부족으로상온에서야적할경우쌀의미질이나빠지기때문에 15 이하의조건에서저장함으로써미질저하를줄일수있으나완전미비율을유지하기위해서는저장고내의상대습도를조절할수있는시설을갖추어야할필요가있다. * 교신저자 : Tel , jjkim74@korea.kr 115

172 PC-22 Niaz Mohammad Rahimi 1, Jong-Tae Song 1, Jeong-Dong Lee 1* 1 High salt concentration is known to affect seed germination, nodule formation, seedling growth, and reduce soybean yield. The objective of the present study was to investigate the effect of NaCl concentrations on water uptake of seeds, germination, seedling growth, fresh weight, and dry weight in soybean. A total of 16 soybean salt tolerant and susceptible accessions (8 G. max and 8 G. soja) were utilized in the present study. Two replicates of seeds were grown within paper towels (30 seeds/replicate) pre-moistened with a range of NaCl solution (0mM,100mM and 200mM) and placed vertically in plastic trays into a dark plant growth chamber at 25 ± 2ºc for 8 days. The water uptake of seeds was measured 24 hours after sowing. The germination was recorded every 24 hours for 8 days. Seedling growth rate and fresh weight were measured 4, 6, and 8 days after germination. The results showed that the highest water uptake percentage was observed in G. soja tolerant accessions at 100mM and 200mM NaCl concentrations. The final germination percentage in all accessions was the same as control at 100mM NaCl concentration, whereas at 200mM germination decreased 29.4%. The G. max accessions showed higher germination percentage than G. soja at 200mM NaCl.The total average seedling growth, fresh and dry weights reduction in all soybean accessions was 49.0% and 10.8%, 41.0% and 10.1%, 51.1% and 23.3% of the control at 100mM and 200mM NaCl concentration, respectively. *Corresponding Author: Tel , jdlee@knu.ac.kr PC-23 김정태 1*, 배환희 1, 이진석 1, 손범영 1, 고영삼 1, 김선림 1, 백성범 1 1 최근내습성관련한인자의도입을위하여옥수수야생종인테오신트 (teosinte) 를이용한연구가진행되고있다. 따라서본연구에서는옥수수야생종인테오신트 (Zea diploperennis 등 6 종 ) 의내습성유용유전자를현재재배되는옥수수로도입가능성을타진하고자수집된테오신트를대상으로유묘기에습해처리후식물체생체량, SPAD 값변화및유전자발현양상을알아보고자수행하였다. 유묘기습해처리에따른테오신트의뿌리생체량은 Zea mays subsp. Parviglumis 을제외한나머지테오신트수집종에서습해처리후 3 일까지는증가하는경향을나타내지만습해처리후 6 일째에서감소하는경향을나타내었다. SPAD 값에따른엽록소함량측정결과에서습해처리후상위엽에비하여하위엽에서측정된 SPAD 값의감소가더크게났다. 그러나다른수집된테오신트에비하여 Zea diploperennis 은유묘기습해처리에따른 SPAD 값의감소율이가장낮게나타났다. 습해처리에따른유전자발현양상은각각의수집된테오신트에서반응정도가차이가있었으며, 내습성관련된 AP2-EREBP-transcription factor 180, Alcohol dehydrogenase 1, Alcohol dehydrogenase 2 등에서발현이높았다. 따라서생체량변화와유전자발현양상을검토해보았을때수집된테오신트에서습해저항유전자가있을것으로추정되었다. * 교신저자 : Tel , kimjt@korea.kr 116

173 PC-24 Tae-Heon Kim, Sumin Jo, Jun-Hyeon Cho, Jong-Hee Lee, You-Chun Song, Dong-Soo Park, Jong-Min Ko, Dongjin Shin * Rice is important cereal crops and staple foods worldwide. Drought is the most serious abiotic stress limiting rice production. However, little progress has been made in the genetic analysis of drought tolerance, because it is a complex trait controlled by a number of genes and affected by various environmental factors. We evaluate rice grain yield of Nagdong and Samgang under two different field conditions, rain-fed, irrigation. Grain yield of Nagdong decreased by 53.3% from 517 kg/10a to 241 kg/10a when compare to irrigation condition. By comparison, grain yield of Samgang decreased by 23.6% from 550 kg/10a to 420 kg/10a. To identify QTLs for drought tolerance, we examined visual drought tolerance (VDT) and relative water content (RWC) using a doubled haploid (DH) population consisted of 101 lines derived from a cross between Samgang (a drought tolerance variety) and Nagdong (a drought sensitive variety). Three QTLs for VDT were located on chromosomes 2, 6, and 11, respectively, and explained 41.8% of the total phenotypic variance. qvdt11, flanked by markers RM26765 and RM287, explained 19.9% of the phenotypic variance with LOD score of 7.1 and an additive effect of 1.0. To determine QTL effects on drought tolerance under rain-fed paddy conditions, seven DH lines were selected according to the number of QTLs they contained. Of the drought tolerance associated QTLs, qvdt2 and qvdt6 did not affect tiller formation, but qvdt11 increased tiller number. Tiller formation was most stable when qvdt2 and qvdt11 were combined. These results suggest that qvdt11 is important for drought tolerance and stable tiller formation under drought stress condition in field. *Corresponding Author: Tel , jacob1223@korea.kr PC-25 Do-Young Bae, Yun-Shil Gho, Ki-Hong Jung Auxin efflux carrier protein family genes play roles in the transport of auxins from the leaves to the roots. however, the biological functions in rice have not been well studied. In this study, we tried to find out functional roles of auxin efflux carrier protein family genes in rice through the diverse transcriptome analysis in the phylogenetic context. These transcriptome data include anatomical meta-expression profiles and differential expression patterns under hormone, abiotic stress, and nitrogen supplement. In rice, there are 12 auxin efflux carrier protein family members and phylogenetic analysis classified these members to four clades. Then, we analyzed the expression patterns in various tissues and organs for ACE genes using both meta-expression data and qrt-pcr. As a result, OsPIN1a, OsPIN1b, OsPIN2, OsPIN5c, and OsPIN9 showed the highest expression in the root. OsPIN1c and OsPIN1d were expressed highly in the young panicles. When nitrogen was supplemented with nitrogen-deficient plants, microarray showed that expression levels of four auxin efflux carrier protein family genes were significantly induced, indicating that auxin efflux carrier protein family in rice are strongly associated with nitrogen utilization. To identify the molecular mechanism for enhancing the nitrogen use efficiency in rice, we are undergoing functional studies for rice auxin efflux carrier protein family genes. *Corresponding Author: Tel , khjung2010@khu.ac.kr 117

174 PC-26 Green Jhang 1*, Gyoungju Nah 1, Sukha Lee Flooding is a one of the most critical factor has influence on decrease in crop growth and productivity. Soybean (Glycine max) is a crop relatively sensitive to wet soil environment, causing poor root respiration and nutrition uptake. MicroRNAs(miRNAs) are known to serve as key regulators of gene expression in relation to biotic and abiotic stress. To investigate the composition of mirnaome of Cheongja-3, known as flood-tolerant soybean, small RNA libraries from control samples (non-flood treated) and flood-treated samples (10 days) were constructed and sequenced by Illumina Hiseq sequencing. After size classification of small RNAs, the 24nt RNAs showed the highest proportion of 26% among the small RNAs ranged from 18 to 30nt. For further investigation to identify candidate mirnas, sequence similarity search was conducted using mirbase ( release 22). A total of 512 sequences were matched to the known mirnas of Glycine max (WS82), and 90 sequences to the mirnas of Arabidopsis thaliana. Of them, 55 sequences were detected in both G. max and A. thaliana. Our results in this analysis will be helpful to identify candidate mirnas in Cheongja-3, as well as the comparative mirnaome study with soybean WS82 and other related species. *Corresponding Author: Green Jhang, Tel , Valenti85@snu.ac.kr PC-27 Zhi-Fang Zuo 1, Hana Jeong 1, Hyeon-Jin Sun 2, Mi-Young Park 2, Hong-Gyu Kang 2*, Hyo-Yeon Lee 1,2 1 2 Plenty of genes are known to regulate the cold response in plants. ICE1 (Inducer of CBF Expression 1) is a regulator of cold-induced transcriptome, which plays an important role in plant cold response pathway. To enhance the cold tolerance of Zoysia japonica, one of the perennial warm-season turfgrass and mainly cultivated in subtropical and tropical Asian countries, it is crucial to understand the cold stress response mechanism in Zoysia japonica. In this study, we presented identification of stress-responsive ZjICE1 from Zoysia japonica and functional characterization in abiotic stress, mainly cold. Our results demonstrated that ZjICE1 shared the typical feature of ICE homologs protein, belonged to nucleic protein. ZjICE1 overexpression transgenic Arabidopsis showed improved tolerance to cold stress by increasing the antioxidant enzyme activity of SOD and POD, free proline content, with a reduction of MDA content, and facilitating the transcripts abundant of cold-responsive genes (AtCBF1, AtCBF2, AtCBF3, AtCOR47A, AtKIN1, and AtRD29A). All of these suggest that ZjICE1 is a positive regulator during cold stress and help to elucidate the cold response cascade in Zoysia japonica, with the purpose of improving cold stress tolerance of Zoysia japonica by molecular breeding programs. *Corresponding Author: Tel honggyu@jejunu.ac.kr. 118

175 PC-28 Cuc Thi Nguyen, Jagadeesh Sundaramoorthy, Gyu Tae Park, Jeong-Dong Lee, Jong Tae Song * Drought and flood are the major abiotic stresses affecting crops yield and production stability. Soybean (Glycine max (L.) Merr.) is an important leguminous crop known as a sensitive to drought and flood. The purpose of present study is to screen genetic variation in droughtand flood-tolerant accessions and utilize them as genetic resources for the development of high yielding drought- and flood-tolerant varieties. In the present study, we used leaf scorch score (LSS) index to identify tolerant accessions. A total of seven hundred and seventy one (386 accessions G. max and 385 accessions of G. soja) accessions of soybean core population in Korea were screened for drought tolerance in greenhouse condition. Out of them, 21 accessions showed very tolerant phenotype for drought screening. In case of flood tolerance screening, a total of three hundred and eighty lines of EMS population were tested and eight lines showed tolerant phenotype. In further studies, the tolerant lines isolated will be utilized for the breeding programme to identify the genetic resources involved in the drought and flood tolerance. Acknowledgement: This work was carried out with the support of Cooperative Research Program for Agricultural Science & Technology Development (Project No. PJ ) by the Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , jtsong68@knu.ac.kr PC-29 Jin Sol Park 1, Hye Jeong Kim 1, Hyun Suk Cho 1, Jae Seong Kim 1, Wan Woo Yeom 1, Jae Yong Han 1, Dae-Jin Yun 2, Young-Soo Chung 1* 1 2 YUCCA6 gene was introduced to produce drought tolerant transgenic soybean plants via Agrobacterium-mediated transformation using the improved half-seed method. The presence of the gene in transgenic plants were confirmed by PCR and Southern blot analysis, and the expression was investigated by RT-PCR. Transgenic line #2, #3 and #5 were tolerant to drought stress while non-transgenic plants were withered completely. Line #2, #3 and #5 were not affected remarkably by water deficit condition and lead to enhanced drought tolerance due to the prevention of cell membrane damage and maintenance of chlorophyll content. Moreover, the enhanced drought tolerance in transgenic lines resulted in reduced transpiration rate and low ROS content. *Corresponding Author: Tel , chungys@dau.ac.kr 119

176 PC-30 박단비, 최지원, 배은경, 최현모, 이효신 * Stress-associated proteins(saps) 을암호화하는 AN1/A20 zinc finger family 유전자는다양한스트레스에반응하여발현되는것으로알려져있다. 본연구에서는현사시나무에서 AN1/A20 도메인을갖는 19 개의유전자 (AN101 AN119) 를분리한다음염과건조스트레스처리에의해 2 배이상발현이변화하는 8 개의유전자를선발하였다. 선발된유전자의발현을각각증가또는억제시킨형질전환현사시나무를만들었다. 건조및염스트레스를처리하고광화학효율을조사한결과, AN101, AN111, AN112 그리고 AN119 의유전자발현이억제된형질전환현사시나무에서내염성의증가를확인하였다. 한편 AN105 의발현이증가된형질전환현사시나무는내건성이증가하였다. 따라서현사시나무의 AN1/A20 zinc finger family 유전자는내염성및내건성이증진된기능성임목을개발하는데유용하게활용될것으로기대된다. 현재, 형질전환현사시나무에서내염성및내건성이증진된기작을구명하기위해유전자발현정보분석등을수행중에있다. * 교신저자 : Tel , hyoshinlee@korea.kr PC-31 Xuefei Yang 1, Jungmin Ha 1,2, Suk-Ha Lee 1,2* 1 2 NACs are plant-specific transcription factors which play crucial roles in plant development and biotic and/or abiotic stress responses. This study was performed to characterize functions of a soybean NAC gene, GmNAC109 in stress response and lateral root formation. DNA sequence of GmNAC109 showed the highest identity with ATAF1 (Arabidopsis Transcription Activation Factor 1) which regulates both abiotic and biotic stress response. In soybean, expression of the GmNAC109 gene was significantly induced by salt stress, especially in root tissue. Similarly, overexpression of GmNAC109 in Arabidopsis plants resulted in higher tolerance to drought and salt stress. The stress response-related genes, such as DREB2A, COR15A and ABI5 were positively regulated by GmNAC109 in the transgenic Arabidopsis plants. Besides, overexpression of GmNAC109 significantly enhanced the lateral root formation which is known to be regulated by auxin pathway and auxin signaling-related genes, ERF5, AXR3 and ARF2 were up-regulated in roots of the transgenic Arabidopsis plants. This indicates that GmNAC109 may also be involve in auxin signal pathway to regulate hairy roots formation. Our results provide a basis for genetic manipulation to develop promising soybean lines by improving tolerance to abiotic stress. *Corresponding Author: Suk-Ha Lee, Tel , sukhalee@snu.ac.kr 120

177 PC-32 Kyu-Chan Shim, Sunha Kim, Hyun-Sook Lee, Yun-A Jeon, Luong Ngoc Ha, Cheryl Adeva, Woo-Jin Kim, Sang-Nag Ahn * Low-temperature germinability (LTG) is one of the most important traits in direct seeding method for rice cultivation. In our previous study, five QTLs controlling the LTG were identified using BC 4F 8 population derived from an interspecific cross between a Korean elite line Hwaseong and Oryza rufipogon (IRGC ). These five O. rufipogon QTL alleles increased the LTG and were located on chromosomes 1, 3, 4, 10, and 11 (qltg1, qltg3, qltg4, qltg10, and qltg11). To examine the interaction between QTLs, we selected one progeny, CR1022, which has both qltg1 and qltg3 O. rufipogon alleles with Hwaseong genetic background. CR1022 was crossed with Hwaseong and 769 F 2 plants were developed. Six different genotype groups for qltg1 and qltg3 were selected from F 2 population using flanking SSR and InDel markers. Seeds of six genotype groups were incubated at 13 in the growth chamber for LTG phenotyping and germinated seeds number was counted. Phenotyping results showed that the O. rufipogon alleles at qltg1 and qltg3 increased the LTG, respectively. Moreover, plants containing both qltg1 and qltg3 from O. rufipogon showed the highest LTG score. A two-way ANOVA indicated significant interaction between qltg1 and qltg3, indicating that the two QTLs possibly regulate the LTG in the same pathway. The qltg3 locus was closely located with the known qltg3-1 (Os03g ) gene. In addition, the sequence variation of qltg3-1 gene was found between Hwaseong and Oryza rufipogon and it might be responsible for the variation of LTG in CR1022. Because qltg1 locus is not yet cloned, further studies on molecular mechanism of qltg1 and qltg3 will be carried out in the future. Hence, understanding the genetic and molecular interaction of these QTLs will be useful in rice research and breeding especially in the development of better direct seeding varieties with LTG. Acknowledgment: This work has supported by the National Research Foundation of Korea(NRF) grant funded by the Korea government(msit)(2017r1a2b ). *Corresponding Author: Tel , ahnsn@cnu.ac.kr PC-33 Je-Hun Moon 1*, Jung-Woo Lee 1, Soo-Cheul Yoo 1,2 1 2 Rice is the staple food for half of the world population and is mainly grown in stress-prone rainfed regions where rice plants regularly suffer from various abiotic and biotic stresses. Rice production is likely to be more unstable in the future due to climate change. To cope with this, varieties capable of increasing stress tolerances need to be developed rapidly. Here, we selected genes and QTLs for climate-change related traits such as Sub1 and AG1 for submergence, DTY for drought, Saltol for salinity, Pup1 for root system and Pb1 for blast resistance. DNA polymorphisms of target genes or QTLs were found by comparing trait-specific SNPs/InDels between target-gene donors and elite recipient varieties using the SNP-Seek( database and the related literatures. To develop the allele-specific markers, SNPs/Indels information were used to design KASP (Kompetitive Allele Specific PCR) and PCR gel-based markers. These developed markers were applied to genotyping segregation populations including donor and recipient varieties for marker validation. The subsequent phenotyping will be performed for five abiotic stresses including and one biotic stress. The allele-specific markers developed in this study would be very useful for developing varieties tolerant to biotic and abiotic stresses. *Corresponding Author: Tel , hunjemoon@naver.com 121

178 PC-34 Sang-Beom Lee 1,2, Gyeong-Jin Kim 1, Yu-Ji Lee 1, Kyu-Won Kim 2, Sang-Ho Chu 2, Ji-Hyun Yoon 1, Buung Choi 1, Ji-Hyock Ryu 1, Byeong-Churl Moon 1, Yong-Jin Park 2*, Sang-Won Park 1* 1 2 Cadmium is one of the toxic substances exposed to the environment, threatening organisms including human beings and major crops through the natural circulation process. Cadmium contaminates most agricultural lands exposed by phosphate fertilizers, irrigation water, waste incinerator, contamination such as mines, etc. In particular, disease caused by cadmium are potential chronic features at the level of low toxicity. Generally, when rice is exposed to heavy metals such as cadmium, enzymes (MAPKKK, MAPKK and MAPK) involved in the phosphorylation reaction are induced in vivo. In particular, MAPK is known to be activated for details of Cd-resistant cultivars than Cd-susceptible cultivars. Therefore, in this study, we conducted genome-wide related research and eqtl analysis for breeding of low-cadmium rice varieties of the rice core-set of Kongju National University. Os01g and Os01g have the same function as the MPK5 (Mitogen-activated protein kinase 5) gene and affect cadmium genes such as OsMTP1 (Cation Diffusion Facilitator protein; Translocation of Zn, Cd and other heavy metals), Os08g (K/ Mg/ Cd/ Cu transporter family protein). As a result of the sequence analysis of Os01g , Group12 had an average cadmium content of μgkg -1 (p <0.0001) which was the highest than the other groups, and amino acid was substituted valine to methionine at the 24,785,497 position. Group4 of Os01g had an average cadmium content of μgkg -1 (p <0.001), which was the highest than the other groups. and it had deletion and amino acid substitution at the 23,980,869 and 23,981,207 position. Thus, the group with high cadmium content of Os01g and Os01g is presumed to be Cd resistant. Also, mutations in these genes are expected to reduce MAPK activity and cadmium content. *Corresponding Author: Yong-Jin Park: Tel , yjpark@kongju.ac.kr Sang-Won Park: Tel , swpark@korea.kr PC-35 Chan Seop Ko 1, Jin-Baek Kim 2, Min Jeong Hong 2, Kyeong Hoon Kim 3, Yong Weon Seo 1,4* Common wheat (Triticum aestivum L.) is the most cultivated crop species in the world. Wheat is frequently exposed to heat stress during grain development periods, which resulted in reduction of grain filling period, starch deposition and also grain size. In this study, plants were subjected to high temperature stress (34 /31, day/night) for 5 days at eight days after flowering. A putative gene which was annotated as barley pr17c precursor and was belonged to the BSP family showed a higher transcriptional level during grain development under heat stress in qrt-pcr. Total 15 BSP family member genes in Triticum aestivum can be grouped into six phylogenic clades, which were further confirmed by the following sequence analyses and motif structures. Based on the 51 identified homologs in seven Poaceae families, they were associated with six phylogenic clades of Triticum aestivum. The defensive role of BSP family in relation to plant defense against pathogenesis has been widely studied. Here, we revealed that BSP family genes are also important responsive genes under the high temperature. Acknowledgement: This work was carried out with the support of the R&D Agenda Agriculture & Technology Development Program (Project No. PJ ) of the Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , seoag@korea.ac.kr 122

179 PC-36 Young-Saeng Kim 1*, Il-Sup Kim 2, Jin-Ju Kim 3, Seong-Im Park The monodehydroascorbate reductase (MDHAR), which is responsible for growth and development and stress response in plants, is a key enzyme in the maintenance of ascorbate (AsA) pool through the AsA glutathione (AsA GSH) cycle and is induced by abiotic stresses. It has highly conserved regions containing FAD- and NAD(P)H-binding domains. In particular, NAD(P)H is a significant electron donor in the AsA GSH pathway. In this context, we introduced RNA interference (RNAi) to determine the functional role of Oryza sativa L. japonica MDHAR isoform 3 (MDHAR3) and developed transgenic (mdhar3) rice plants in which the NAD(P)H domain was silenced. The mdhar3 rice plants were more sensitive to salt stress than the wild-type (WT) plants. In addition, the mdhar3 rice plants showed decreased ability for environmental adaptation because of an imbalance in the redox homeostasis and reduced AsA pool. These plants showed increased hydroperoxide levels and ion leakage, and decreased chlorophyll content and ascorbate/dehydroascorbate ratio under the paddy field conditions; they also exhibited a reduction in the total biomass and grain yield. These results suggest that MDHAR3 plays a critical role in the intrinsic resistance, as well as in the sensitivity of seed maturation and productivity, of rice plants to environmental stresses, thereby, indicating the functional importance of NADH in MDHAR activity, in vivo and in vitro. *Corresponding Author: Tel , kyslhh1228@hanmail.net PC-37 Sun Ha Kim, Hyun-Sook Lee, Kyu-Chan Shim, Yun-A Jeon, Anh Quynh Le and Sang-Nag Ahn * Seed germination is being delayed or inhibited under several kinds of stress such as temperature, salt, and osmotic pressure. High germination rate for low-temperature is an important factor in growing rice. In direct-seeded method in rice, low-temperature germinability is considered as one of the factors for stable plant stand establishment in temperate regions and high altitude areas. Previously, we detected the qltg1 as the quantitative trait locus (QTL) that plays a vital role in controlling tolerance to a low temperature in seed germination stage using progenies derived from a cross between Oryza sativa (cv. Hwaseong) and Oryza rufipogon (Rufi). The qltg1 was detected and located between in CRM23-CRM15 on chromosome 1 within 56.4 kb region harboring 10 genes. To identify the genes targeted by qltg1, the expression profiles of the identified candidate genes and germination behavior of qltg1 under different low-temperature condition were investigated and compared to HS, Rufi, and TR5 (BC 3 F 7 ). These results indicated that qltg1 showed tolerance for several abiotic stresses such as salt, drought and low temperature. The qltg1 for low-temperature germinability would be useful in rice breeding programs especially in the development of lines possessing low-temperature germinability. Acknowledgement: This work was carried out with the support of "Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ )" Rural Development Adiministration, Republic of Korea. *Corresponding Author: Tel , ahnsn@cnu.ac.kr 123

180 PC-38 Young-yi Lee *, Hyung-jin Baek, Jung-yoon Yi, and Mun-seop Yoon The seeds of maize (Zea mays L.), sorghum (Sorghum bicolor), and cowpea (Vigna unguiculata) were examined the germination rate after 10 years of long-term storage (-18 C) conservation. For maize seeds, 4,463 accessions were examined and germination rate of 1,512 accessions was decreased with below 15% of initial germination rate. For 183 accessions of maize, germination rate was decreased with above 15% of initial germination rate after 10 years of long-term storage, which is needed to be rejuvenated. Germination rate of 1,404 accessions was increased and showed no change for 1,364 accessions after 10 years of long-term storage. For sorghum seeds, 830 accessions were examined and germination rate of 345 accessions was decreased with below 15% of initial germination rate. For 21 accessions of sorghum, germination rate was decreased with above 15% of initial germination rate after 10 years of long-term storage, which is needed to be rejuvenated. Germination rate of 368 accessions was increased and showed no change for 96 accessions after 10 years of long-term storage. For cowpea seeds, 497 accessions were examined and germination rate of 127 accessions was decreased with below 15% of initial germination rate. For 34 accessions of cowpea, germination rate was decreased with above 15% of initial germination rate after 10 years of long-term storage, which is needed to be rejuvenated. Germination rate of 238 accessions was increased and showed no change for 98 accessions after 10 years of long-term storage. Acknowledgement: This study was carried out with the support of "Development of long-term conservation techniques for several vegetatively propagated crops and short-lived seed species, and quality control for long-term conserved germplasm (Project No.PJ011996)", National Institute of Agricultural Sciences, Rural Development Administration, Republic of Korea. *Corresponding Author: , PC-39 Won-Kyung Lee 1, Jee Young Park 1, Sang-Choon Lee 1, Ho Jun Joh 1, Jaehyeon Jeon 1, Kihwan Song 2*, Tae-Jin Yang 1* 1 2 For identification of chilling tolerance related genes, three chilling susceptible Cucumber inbred lines and two chilling tolerant Cucumber inbred lines were used as materials and sequenced using Illumina Miseq. Reference genome sequences were collected from NCBI GenBank and annotation data was downloaded from cucumber genome database. Through read mapping analysis to reference Cucumber genome data, all nuclear, chloroplast, and mitochondrial genome sequences of five Cucumber inbred lines were compared to each other. As a result totally 26,579 common polymorphic sites were identified between chilling tolerant and susceptible lines. Among all of 26,579 polymorphic sites, 1,182 sites were located in CDS regions of nuclear genome and two polymorphic sites were in intron regions of mitochondrial genome sequences. We also conducted candidate gene approach to identify the cold tolerance gene. A total of 16 genes were selected for candidate cold tolerance responsible genes in two Cucumber inbred lines. We developed 12 dcaps markers one from each candidate gene based on these polymorphic analysis results. Candidate genes and developed markers will be valuable resource for molecular breeding of Cucumber. This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Agri-Bio industry Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (grant number HD0b0). *Corresponding Author: Tel , khsong@sejong.ac.kr, tjyang@snu.ac.kr 124

181 PC-40 Muhammad Rauf, Do Yoon Hyun *, Sejong Oh, Myung-Chul Lee, Yu-Mi Choi, Sukyeung Lee Direct-seeded rice cultivation (DSC) is getting farmer s attention due to its low water requirements and labor s cost. In temperate regions, DSC is followed by flooding by irrigation or heavy rainfall creating anaerobic conditions for seed germination. In such anaerobic conditions, successful seedling establishment requires rapid coleoptile growth to ensure access to oxygen near the water surface. However, not all rice varieties can tolerate anaerobic conditions; only tolerant accessions with strong anaerobic germinability (AG) can germinate and elongate their coleoptiles to escape from anaerobic conditions. Due to the limited availability of highly tolerant genotypes, there is an urgent need to select diverse rice germplasm with enhanced AG. In this study, we evaluated 185 rice accessions from six subpopulations for germination rate (AGR) and coleoptile length (ACL) under anaerobic conditions. The highest average AGR (60%) and longest average ACL (2.13 cm) were observed in tropical japonica and temperate japonica germplasm, respectively. We identified highly-tolerant and -susceptible accessions and compared relative expressions of AG-related genes with well known tolerant variety KHO via quantitative real-time PCR. The proton pyrophosphatase (OVP3) and rice alpha-amylase (RAmy3D) were expressed at significantly higher levels in the strong accessions than KHO and susceptible accession. In general, OVP3 was expressed at the highest levels in all strong accessions, whereas the expression level of rice ethylene-response element binding protein (OsEREBP1) and alcohol dehydrogenase 1 (ADH1) did not significantly differ among accessions. These findings could be helpful for breeders and lay the foundation for further genetic analysis. *Corresponding Author: Tel , dyhyun@korea.kr PC-41 이다솜 1, 이진원 1, 윤상대 1, 소문수 2, 오성앵 1, 박순기 1* 1 2 식물은성장하면서다양한환경스트레스에노출되며, 이러한스트레스는식물생육에영향을끼친다. 현재계속되는지구기후변화로인한기온상승과극단적기상변동등은농업에부정적인영향을미쳐작물의수확량감소를초래하는직접적인원인이되고있다. 따라서식물의비생물학적스트레스저항성을증진시키는것은환경조건의변화시에도식물생장과발달을유지하여안정적생산을가능하게해줄것이다. 벼의고온스트레스저항성증진할목적으로, 본연구에서는애기장대에서유래된비생물학적스트레스저항성관련후보유전자 6 종 (NDPK2, GolS1, PRE1, YUCCA6, Hsp101, CBF1) 들을벼유래화기조직특이적및상시발현을유도하는 4 종의프로모터 (TDF1, RMP1, OsLPS1, UBQ14) 들과결합시킨형질전환용벡터들을제작하였다. Agrobacterium 을이용한조직배양으로미성숙배로부터캘러스를유도시켜재분화를통해벼형질전환체들을육성하였으며, T0 식물체 genomic DNA 의 PCR 분석을통해유전자도입을확인하였다. 후대에서의저항성검정과 PCR 분석을통해 single copy 가들어간식물체들을선발하고 T2 세대에서유묘기, 출수기등고온스트레스에취약한발달단계에서저항성을검정한다. 다양한후보유전자들을대상으로고온저항성증진에효과적인유전자에관한연구결과들은향후지구온난화대비벼품종육성을위한기초자료로유용하게활용될것으로기대된다. * 교신저자 : Tel , psk@knu.ac.kr 125

182 PC-42 MyeongEun Choe *, Jee Yeon Ko, Seuk Bo Song, Do Yeon Kwak Sorghum is highly productive crop plant, which can be used for human food, livestock feed and alternative energy resource. The plant has a great adaptation potential to drought, high salinity and high temperature, which are important characteristics of crop growth. Salinity is an abiotic stress that limits both growth and yield of crops. Genotypic differences in salt tolerant exist and exploiting genetic variability to identify salt tolerant genotype is one of the strategies used to overcome salinity. For this purpose some experiment was carried out to evaluate the genetic variation of 136 sorghum genotypes for NaCl salinity response at germination and early seedling stages. Experiments were conducted in Petri plates and plots with 250mM NaCl levels to validate screening tools. Physiological parameters such as germination, shoot and root development were analyzed to identify the salt tolerant of representative line. Nampungchal and Btx623 were categorized as tolerant, SC372 were as sensitive ones. The results affirmed the presence of genotypic variation among the sorghum genotypes for sal tolerance. the selection of suitable lines that can be recommended for saline areas to improve yields. *Corresponding Author: Tel , cme0807@korea.kr PC-43 Sang Dae Yun 1, Jin Won Lee 1, Da Som Lee 1, Sung Aeong Oh 1, Moon-Soo Soh 2, Soon Ki Park 1* 1 2 As the world population and grain demand have been increasing, food security is the most urgent matter we face. In addition, environments of the plant growth are predicted to be changed due to global climate changes in the coming decades, which will seriously affect sufficient crop yield. There have been significant efforts to improve plant fitness that can withstand various stress conditions. There have been reports about positive relationship between C-repeat-binding factors(cbf) genes expression and cold acclimation. It has been also known that plants overexpressing the CBF1 gene exhibit growth retardation and late flowering. In this study, we investigated whether CBF1 gene also plays a role for heat tolerance in Arabidopsis. To this end, we generated transgenic lines harboring an overexpression vector proubq14-cbf1. We tested 10 day-old seedlings at heat stress conditions and checked their survival rates. Our preliminary results suggest that overexpression of CBF1 gene confers enhanced heat tolerance at seedling stages. Based on this result we will continue to test heat stress conditions during reproductive stages. *Corresponding Author: Tel , psk@knu.ac.kr 126

183 PC-44 Myeong-Hyun Min 1, Sang-Ho Chu 2, Kyu-Won Kim 2, Yong-Jin Park 1,2* 1 2 Using 378 Asian cultivated rice varieties, we performed GWAS and eqtl analysis independently. The result of GWAS showed that a number of significantly associated SNPs with preharvest sprouting (PHS) response tested in field for two years. eqtl analysis was performed to identify the genetic variants associated with the expression of each gene. Some of the significantly associated SNPs with PHS response by GWAS were also significantly associated eqtl of certain genes indicating the SNPs are involved in PHS response as a way of influencing the expression of specific genes. Keywords: preharvest sprouting, GWAS, eqtl, Asian cultivated rice *Corresponding Author: Tel , yjpark@kongju.ac.kr PC-45 Hye-Jin Yoon *, Seung-Bum Lee, and Yeon-Hee Lee We have developed the transgenic pool of over-expressing full length genes in rice to investigate the functions of rice genes and to create the resources applicable for the molecular breeding program as a fundamental approach for crop improvement. The overexpression vector pb2gw7 was modified for the construction of destination vector in the use of the insertion clones of a cdna library. It is constructed from the stress-related genes induced in rice under the condition by the treatment of both abiotic and biotic stresses. All the retrieved sequences of clones from the cdna library were scanned and determined using data of Rice Genome Annotation Project. Only a full-length insertion fragments were selected from the clones of the constructed cdna library, and the selected full-length genes for the transgenic lines are approximately over 400. The Agrobacterium strain LBA4404 harboring the modified pb2gw7 vector was introduced into embryogenic rice calli (Oryza sativa L. Japonica), and then the transgenic lines were preliminarily obtained by Agrobacterium-mediated transformation and regeneration method. Phenotypic observation of the positive T1 transgenic plants showed that a few of these transgenic lines exhibited potentially obvious phenotypes (yellowish leaves, more branches etc.). Subsequent phenotypic analysis is underway to demonstrate that these phenotypes were due to the overexpression of rice genes. *Corresponding Author: Tel , hyejinyoon@korea.kr 127

184 PC-46 Thuy Thi Le 1, Minsu Kim 1, Rupesh Tayade 1, Niaz Mohammad Rahimi 1, Jong-Tae Song 1, Jeong-Dong Lee * 1 Soybean (Glycine max) and wild soybean (G.soja) are annual legumes of Fabaceae family. Plant height is an important characteristic trait in soybean germplasm and cultivated soybean for evaluation. In this study, four elements were used to assess variation of early plant height in wild and cultivated soybean. Wild accessions 375 and 395 cultivated soybean used for a field experiment to identify and evaluate for plant height at early stage. The experiment was laid out in complete randomized design (RCBD) with three replications. Secondly, we evaluated the correlation between planting dates to the plant height in wild soybean. Third, the effect of four plant hormones GA 3, GA 3+4, GA 4+7 and prohexadione-calcium (inhibitor of GA synthesis) were used with four different concentration 0, 10, 20, and 40µM in three different plant groups for instance ( fast-growing, slow-growing and cultivated soybeans). Finally, we evaluated the segregation pattern for plant height when crossing between fast-growing and slow-growing accessions. In addition to plant height we examine other agronomic traits, such as the number of nodes, the number of stem diameter, the number of branches per plant after 30 days of planting. The result of tendency showed that the bioactive GA 3 and GA 3+4 were detectable to the elongation increased of three plant groups. Concentration 40µM. was found to be best among four treatments. Our results provide important information for rapid growth trait in wild soybean and a useful trait for evaluation plant height in soybean. *Corresponding Author: Jeong-Dong Lee (jdlee@knu.ac.kr) PC-47 Thuy Thi Le 1, Minsu Kim 1, Danim Jo 1, Rupesh Tayade 1, Niaz Mohammad Rahimi 1, Bo-Keun Ha 2, Jong-Tae Song 1, Jeong-Dong Lee 1* 1 2 Soybean (Glycine max) and wild soybean (G. soja) are annual legumes of Fabaceae family. Understanding genetic and environmental effect on plant height is important for soybean production. In this study, core collections including 375 wild accessions and 395 cultivated soybeans were used for a field experiment to identify and evaluate for plant height at early stage. The experiment was laid out in complete randomized design (RCBD) with three replications. Secondly, we evaluated the correlation between planting dates to the plant height in wild soybean. Third, plant hormones, GA 3, GA 4, and prohexadione-calcium (inhibitor of GA synthesis), were used to figure out the effect on plant height with four different concentrations 0, 10, 20, and 40µM in three different plant groups (fast-growing, slow-growing and cultivated soybeans) for instance. The result showed that there was big variation in early plant height in wild soybean and the bioactive GA 3 was detectable to the elongation increased of three plant groups. Our results provide important information for rapid growth trait in wild soybean and a useful trait for evaluation plant height in soybean. *Corresponding Author: Jeong-Dong Lee (jdlee@knu.ac.kr) 128

185 PC-48 Jang-Hwan Yu 1, Hyeong-Jun Park 1, Swapan Kumar Roy 1, Soo-Jeong Kwon 1, Ju-Young Choi 1, Seong-Woo Cho 2, Hong-Sig Kim 1 and Sun-Hee Woo 1* 1 2 Waterlogging (WL), a major environmental stress, is a severe constraint on crop growth and productivity in many regions and situations. Waterlogging can reduce the photosynthesis rate, induce oxidative stress, accelerate leaf senescence, inhibit plant growth and finally lead to crop yield loss. The present study was conducted to investigate the morpho-physiological and proteome responses of three- and five leaf stage of soybean to WL stress. The domestic cultivar, Uram was used to test the waterlogging stress. In the experiment of 3-leaf stage soybean, stem length, chlorophyll contents were decreased when the plants were exposed to WL stress. The 5-leaf stage experiment was similar to the 3-leaf experiment. In the waterlogging for 4 days, the stem length and chlorophyll contents showed significant change among other treatments. Characteristic related to leaf showed treatments was smaller than control regardless to soils. The results observed from experiment 1, 2 and 3 were considered to be influenced by the waterlogging stress more in the 5-leaf stage soybean, and as the waterlogging treatment progressed, the waterlogging stress influenced the growth difference between control and treatment. A total of 30 protein spots were analyzed using LTQ-FI ICR MS. As a result, 9 proteins were up-regulated in the treatment group and 4 proteins were down-regulated. Analysis of LTQ-FI ICR MS showed that 50% of the proteins involved in RNA processing, translation, biological process. Malate dehydrogenase protein and Glyceraldehyde-3-phosphate dehyderogenase protein increased the level of protein expression in 3 and 5-leaf stage under waterlogging stress. These proteins are known to function as antistrss agents. The expression of oxygen-evloving enhancer protein 1 related to photosynthesis was increased in treatment than control. Superoxide dismutase protein related to response to oxidative stress showed high expression level in 5-leaf stage treatment. These results suggest that waterlogging directly impairs photosynthesis and photorespiration. Acknowledgement: This work was supported by a grant from the Agenda project (No. PJ ) of the Rural Development Administration, Republic of Korea *Corresponding Author: Tel , shwoo@chungbuk.ac.kr PC-49 Hyun-Jin Jung 1, Sang-Heon Choi 1, Swapan Kumar Roy 1, Soo Jeong Kwon 1, Seong-Woo Cho 2, and Sun-Hee Woo 1* 1 2 Waterlogging is a common adverse environmental condition that limits plant growth and one of the major abiotic stresses affect in sesame (Sesamum indicum L.) yields resulting in increases of relative ion leakage, lipid peroxidation and in vivo H 2 O 2 content. The purpose of this study was to explore the protein expression patterns of sesame leaves under waterlogging stress. The plant height, stem length, chlorophyll content exhibited gradually decrease while chlorophyll and H 2O 2 content increased significantly in response to waterlogging stress. More than 300 protein spots were detected on 2-DE from 10-leaf growth stage and 20 protein spots were differentially altered, and their abundance was significantly responsive to waterlogging treatment, with more than a 1.5-fold change in intensity. In case of flowering stage, more than 400 protein spots were identified and a total of 31 protein spots that exhibited more than a 1.5-fold changes in intensity. Of 31 proteins 16 proteins were found to be up-regulated and 15 proteins were down-regulated under waterlogging stress. These findings shed light on the complex mechanisms underlying waterlogging tolerance in sesame. Acknowledgement: This work was supported by a grant from the Agenda project (No. PJ ) of the Rural Development Administration, Republic of Korea *Corresponding Author: Tel , shwoo@chungbuk.ac.kr 129

186 PC-50 Hae-Ryong Jeong 1, Swapan Kumar Roy 1, Soo-Jeong Kwon 1, Ku-Hyun Kwon 1, Seong-Woo Cho 2, and Sun-Hee Woo 1* 1 2 Waterlogging of soil is a major limiting factor for crop growth in humid regions. Prolonged rainy period or heavy rainfall in the field with poor soil drainage significantly reduces the seed yield of grain legumes. To explore the morpho-physiological and unravel the molecular tolerance mechanism of two- and five leaf stage of Azuki bean to waterlogging stress. The plant height, stem length, chlorophyll contents were decreased when the plants were exposed to waterlogging stress. In the 2-leaf stage, more than 400 protein spots were detected on 2-D gels, and quantitative image analysis revealed a total of 43 protein spots that exhibited more than a 1. 5-fold changes in intensity whereas 23 differentially expressed proteins were successfully analyzed using MALDI-TOF/TOF MS analysis. Among these proteins, a total of 14 proteins showed increased expression, and 9 proteins showed decreased expression in the treated samples compared to their levels in untreated seedlings. However, in the 5-leaf stage proteome, 29 protein spots were found to be expressed differentially upon waterlogging stress, with more than 1.5-fold differences in abundances between the control and waterlogging-treated samples. A total of 16 proteins were increased significantly while 13 proteins were decreased abundances. The abundance of the most identified protein species from the leaves that function in stress response and metabolism was significantly enhanced, while protein species involved in transcription and regulation were severely reduced. Acknowledgement: This work was supported by a grant from the Agenda project (No. PJ ) of the Rural Development Administration, Republic of Korea *Corresponding Author: Tel , shwoo@chungbuk.ac.kr PC-51 Kang-Heum Cho 1*, Jungmin Ha 1, Moon Young Kim 2, Sangrea Shim 1 and Suk-Ha Lee Basic region-leucine zipper (bzip) transcription factor family is one of the largest families in plant species, and is known to play crucial roles in plant development from various signaling responses to organogenesis. Because of its significance, genomic studies of bzip transcription factor (TF) have been conducted in many important plant species, such as Arabidopsis, rice and maize. Mungbean (Vigna radiata (l.) R. Wilczek), although recognized as one of the major crops in Asia for its high level of protein and vital micronutrients, has not been studied for its identification and characterization of bzip TF. Recently, reference genome sequence of mungbean has been published. In this study, based on the mungbean genome sequence and annotations, bzip TF genes in mungbean were identified using sequence similarities of known Arabidopsis bzip TFs, and further verified with domain search. Based on the neighbor-joining tree constructed from bzip TF sequences of Arabidopsis and mungbean, mungbean bzip TF clades were classified and other conserved motifs were analyzed. Additionally, possible gene duplications and sequence variations within regions of bzip TF sequences were found with synteny analysis of mungbean genome. To check expression level variations of bzip TF genes among four tissues of mungbean, including leaf, flower, pod and root, expression heatmap was built using RNA-Seq data. With the first genome-wide study of bzip TF genes in mungbean, the data can further be utilized to elucidate the mechanisms behind various stress responses and development of mungbean. *Corresponding Author: Tel , sukhalee@snu.ac.kr 130

187 PC-52 Franklin Hinosa Mancia 1, Ki-Byung Lim 2, Min Hee Ju 3, Hyo Bin Park 3, Lee Ye Jun 3, Hye Jin Seo 3, Min Hee Kim 3, Woo In Jung 3, Woo Chul Ju 3, Yoon-Jung Hwang 3* Chrysanthemum is popular for the beauty of its flowers and the health benefits it offers. The good keeping quality with thedifferent varieties of flowers has made its mark in the floricultural industry in Korea and in the other parts of the world. Karyomorphological study is performed on three tetraploid species of chrysanthemum by employing fluorescence in situ hybridization (FISH) technique using rdna probes. The three species have a chromosome number of 2n=4X=36. FISH results showed 8 and 4 signals of 45S and 5S rdna in Chrysanthemum boreale, Chrysanthemumindicum, and Chrysanthemumzawadskii. The number and distribution of rdna signals which are all located in metaphase chromosomes between C. boreale and C. indicum are more conversed. This may suggest that the genomic components of the 2 tetraploids are mostly homogeneous. The karyotype formulae of three polyploids are 27m+9sm+0st+0t, 23m+13sm+0st+0t, and 25m+9sm+2st+0t. The karyotypes of three species are found to be symmetric. This implies that the three chrysanthemums are primitive or primordial species. The cytological information coupled with molecular analysis are vital for the elucidation of the genetic make-up of plants and to better understand their phylogenetic relationship. Keywords: FISH karyotypeploidy, symmetry, rdna *Corresponding Author: hyj@syu.ac.kr; Tel PD-01 Da-Hye Kim 1, SangKyu Park 1, Jong-Yeol Lee 1, Sun-Hyung Lim 1* 1 Anthocyanins are important pigments that influence the quality of fruits and flowers. Anthocyanin biosynthesis is regulated by transcription factors and other proteins working in concert to finely tune the expression of genes involved in the flavonoid biosynthetic pathway. In rice (Oryza sativa), the R2R3 MYB transcription factor (TF) OsC1 and a bhlh TF, OsB2, were previously reported to control anthocyanin biosynthesis in vegetative tissues and seeds, respectively; however, the regulatory mechanisms of the anthocyanin biosynthesis TFs remain largely unknown. In this study, we identified OsBBX14, a homolog of Arabidopsis thaliana B-box domain protein 22 (AtBBX22), and investigated its function. The transcript level of OsBBX14 was high in pigmented rice seeds and gradually increased as the seeds matured. The ectopic expression of OsBBX14 in Arabidopsis resulted in a dramatic increase in anthocyanin accumulation in seedlings. Using a steroid receptor-based inducible activation system, OsBBX14 was found to partner with OsHY5 to directly activate the transcription of OsC1. Furthermore, OsBBX14 was found to physically interact with OsHY5; the second B-box domain of OsBBX14 and the bzip domain of OsHY5 were sufficient for their interaction. Taken together, these results show that OsBBX14 interacts with OsHY5 to induce and fine tune the expression of OsC1, and thereby regulates anthocyanin biosynthesis. *Corresponding Author: Tel , limsh2@korea.kr 131

188 PD-02 Andre Silvanovich 1, Carl Garnaat 1, Colton Kessenich 1, Qing Tian 1, Bradley Comstock 1, Marianne Malven 1, Will Urquhart 1, Hye Jung Hyun 2*, Jonghyun Kwon Molecular characterization of crops produced by transformation has traditionally been conducted using Southern blot analysis which has been used to determine T-DNA insert and copy numbers, the presence or absence of backbone (sequence outside of the T-DNA) and to demonstrate generational stability of the T-DNA insert. With the advancement of high-throughput Next Generation Sequencing (NGS) technology, efficient characterization of the transgene incorporated into the plant genome is now feasible by sequencing the entire plant genome. By generating NGS data with sufficient coverage depth followed by sequence read mapping to the plasmid vector, inserted plasmid sequence and flanking genomic sequence are identified. Through the analysis of mapped reads over multiple breeding generations, conclusions equivalent to those of Southern blot analyses, including insert number, copy number, absence of backbone and generational stability can be drawn. Stacked products are produced by conventional breeding of fully characterized single events to combine two or more GM traits into a single plant. Considering the nature of conventional breeding, a repeated full molecular characterization of each insert is not necessary as the transgene DNA behaves no differently than endogenous plant DNA. Therefore, transgenic events in a breeding stack can be confirmed by comparing the fingerprint, a blot that demonstrates the presence of an intact T-DNA insert and flanks, between the stack and the parental single event. Same conclusions can also be obtained through direct sequencing of the T-DNA insert and flanking genomic DNA in the stack when compared to the T-DNA insert and flanking genomic DNA in the parental single. Due to recent advances of DNA sequencing and bioinformatics technology, there is an emerging opportunity to use NGS for the molecular analysis of both single event and stacked event transgenic crops. NGS and direct sequencing yield conclusions that are comparable to those of Southern blotting without radioactive hazards and artifacts that are frequently observed in Southern blotting. *Corresponding Author: Tel , hye.jung.hyun@monsanto.com PD-03 Yinbo Ma, Xiaona Yu, Su Ryun Choi, Dong Hyun Kang, Lu Lu, Man-Sun Kim and Yong Pyo Lim * Radish, Raphanus sativus (2n = 18), belong to the Brassicaceae family, is one of the main economic vegetable in the world. However, the continuously threatened by devastating diseases,climatic variation and environmental stress which are affecting yield and quality of radish. In this study, we conducted genome-wide analyses of radish for two types of pattern recognition receptors which mediate a wide range of processes, including development, disease resistance, initiate an immune response, hormone perception, and self incompatibility. A total of 44 receptor-like kinase (RLK) and 10 of receptor-like protein (RLP) genes are identified in the genome of radish. RLK and RLP gene families each include different s-locus, G-lectin, PAN-2 and kinase functional domains. While a large number of RLPs resembling the extracellular domains of RLKs are also found in the radish genome. A phylogenetic analysis separated these genes into four groups. Group 1and 3 mainly comprise of kinase domain and group 2 mainly comprise of G-Lectin domain and group 4 with PAN domain. Characterizing this gene family will be useful for the genetic improvement of radish. Keywords: Raphanus sativus, receptor-like kinase (RLK), receptor-like protein (RLP), phylogenetics *Corresponding Author: yplim@cnu.ac.kr 132

189 PD-04 Sang Chul Choi, Yun Gyeong Lee, Yu-Na Kang, Kyeong Min Kim, Changsoo Kim * Sorghum bicolor (L.) Moench contains various phenolic compounds such as anthocyanin. We classified eleven sorghum accessions into five groups by seed color and measured antioxidant activity as well as the contents of total phenolic compounds (TPC) and anthocyanin in sorghum grains. The seed color was related to TPC content, but not to monomeric anthocyanin content. Moreover, the overall patterns of antioxidant activity levels in DPPH or ABTS assay were similar to those of the TPC content. We analyzed statistical correlations between TPC and anthocyanin contents, and antioxidant activities. The TPC and anthocyanin contents were statistically significant for positive correlation (P < 0.05), and the TPC content showed strong positive correlation against the DPPH and ABTS antioxidant activities. We expect that our results provide the basic data for breeding of sorghum varieties containing large amounts of antioxidants. Acknowledgement: This work was supported by National Agricultural Genome Program (Project No. PJ , Rural Development Administration, Republic of Korea). *Corresponding Author: Tel , changsookim@cnu.ac.kr PD-05 Eun Ji Lee 1, Jun Hyeon Cho 1*, Ji Yoon Lee 1, Ju Won Kang 1, Young Ho Kwon 1, Soo Min Cho 1, Jong Hee Lee 1, Dong Soo Park 1, You Chun Song 1, Jong Min Ko 1 1 This study was carried out to investigate the physicochemical properties and eating quality under freezing temperature storage in low amylose content rices. For textures of eating quality, the five cooked rice samples were restored by microwave for 2.5 mins from storage of freezing temperature(-18 ) for 6, 24 and 48 hours. In physicochemical analysis, the amylose contents were ranged from 5.14 to 16.62%. And Baekogchal (amylose 5.14%) showed the lowest value in peak, hot paste and cold paste viscosity as it is glutinous rice. Baegjinju (amylose 9.75%) and Saeilmi (amylose 16.62%) were the highest in breakdown viscosity and setback viscosity, respectively. The water absorption index of Baekogchal was the highest level. In eating quality, all the cooked rice samples showed increased textural properties except hardness after 6 hours of freezing storage. Interestingly, the hardness of Miho was increased while the others were decreased after freezing storage. Furthermore, Miho showed not much difference compared to other rices in springiness, gumminess, chewiness and cohesiveness under the freezing storage. Under the freezing and thawing conditions. Miho, Baegjinju and Saeilmi showed increased lightness while Baekogchal was decreased after 48 hours. In this study, we observed that Miho showed a good textural properties for eating quality and color values under freezing storage. Therefore Miho can be more acceptable in the refrigerate foods such as lunch box and processed freezing foods which are distributed under the low temperature. *Corresponding Author: Tel , hy4779@korea.kr 133

190 PD-06 Yang Qin, Ye-Jin Jang, Kong-Sik Shin, Tae-Sung Park, Myung-Ho Lim * Gene editing technologies such as transcription activator-like effector nucleases (TALENs) and CRISPR/Cas9 systems have been developed to create targeted DNA double-strand breaks (DSBs) in many crop plants. DSBs are mainly repaired by error-prone nonhomologous end joining (NHEJ), which often caused small insertions or deletions at break site to generate knockout mutations. Here, TALENs were engineered to target and disrupt the herbicide-resistance bar in two donor plants of Bt-resistance transgenic rice and herbicide-resistance transgenic rice. A total of 10 and 30 of rice plants were respectively regenerated from both donors. Sanger sequencing and bar elisa analyses indicated that four and 18 mutant plants from Bt-resistant rice and basta-resistant rice respectively showed various indel mutations and SNPs at target gene, but not at TALEN target site. Mutant plants carrying only the desired DNA sequence change but not the TALEN transgene would be selected from segregations in the T1 and T2 generations. These TALEN-mediated bar gene knockout mutant lines will be used to study substantial equivalence to non-transgenic donor rice variety Dongjin in the further study. *Corresponding Author: Tel , mlim312@korea.kr PD-07 Eun-Seok Oh and Man-Ho Oh * Proteinpost-translational modification by phosphorylation is essential for the activity and stability of proteins in higher plants and underlies their responses to diverse stimuli. There are more than 300 leucine-rich repeat receptor-like kinases (LRR-RLKs), a major group of receptor-like kinases (RLKs) that plays an important role in growth, development, and biotic stress responses in higher plants.to analyze auto- and transphosphorylation patterns and kinase activities in vitro, 43 full-length complementary DNA (cdna) sequences were cloned from genes encoding LRR-RLKs. Autophosphorylation activity was found in the cytoplasmic domains (CDs) of 18 LRR-RLKs; 13 of these LRR-RLKs with autophosphorylation activity showed transphosphorylation in Escherichia coli. BRI1-Associated Receptor Kinase (BAK1), which is critically involved in the brassinosteroid and plant innate immunity signal transduction pathways, showed strong auto- and transphosphorylation with multi-specific kinase activity within 2 h of induction of Brassica oleraceae BAK1-CD (BoBAK1-CD) in E. coli; moreover, the carboxy-terminus of LRR-RLKs regulated phosphorylation and kinase activity in Arabidopsis thaliana and vegetative crops. *Corresponding Author: Tel , manhooh@cnu.ac.kr 134

191 PD-08 안억근 1*, 현웅조 1, 정국현 1, 원용재 1, 홍하철 1, 박향미 1, 장재기 2, 이정희 1, 이점호 1, 성낙식 1, 서정필 1, 오세관 1, 윤미라 최근쌀과잉생산과소비감소의구조적문제로인해농가소득감소, 정부재정부담가중등으로한시적으로쌀생산조정제가 논타작물재배지원사업 으로시행되고있다. 이에풍흉에따라취사선택할수있는사료용과가공특성겸용용도다양화품종개발이요구되고있다. 또한세계적으로비만및성인병예방기능성간편식품의수요가증가추세에있으며, 우리나라에서도건강기능성이우수한발아현미를이용한간편식제품화에관심이증대되고있다. 발아현미는발아과정에서질감이부드러워지고 GABA 등기능성성분이증가되어치매, 비만및성인병예방에효과적인식품이다. 이번에개발한거대배아미 큰품 은출수기가 8 월 10 일이고기존거대배아미인 큰눈 에비해흰잎마름병 ( 균계 K1, K2, K3), 줄무늬잎마름병저항성을가지고있어내병성이강화되었고수량성 (534 kg /10a) 이 큰눈 에비해 10% 향상되었다. 또한동시발아율이높고제품화시쌀눈탈락율이낮아발아가공에적합한품종이다. 또한 큰눈 에비해외관품위가맑고깨끗하며현미의폴리페놀등항산화성분과항산화활성은 큰눈 보다높아국민건강증진및기능성가공식품소재로활용할수있어재배농가및가공업체소득증대에기여할것으로기대된다. 덧붙여곡실의특성을다양화할수있는소재로도활용가능하여사회경제적여건에따라변화무쌍한시대에용도를다변화할수있는품종개발에도이용가능하다. 본연구는농촌진흥청연구사업 ( 세부과제명 : 쌀용도다양화를위한맞춤형벼품종개발, 세부과제번호 : PJ ) 의지원에의해이루어진것임. * 교신저자 : Tel , okahn@korea.kr PD-09 Yoon Hee Jang, Do Hyeon Gwon, Ju Hun Noh, Je Dong Kim, Kyung-Min Kim * According to the Food and Agriculture Organization (FAO) statistics, GM crops are now commercially planted on about 100 million hectares in some 22 developed and developing countries. Recent global abnormal weather, desertification associated with global warming, dry land soils, and salinization of ground water associated with the large-scale agricultural irrigation agriculture all demonstrate that the agricultural environment is deteriorating rapidly. In agriculture, the rice is the one of important things. Many farmers and scientists have long tried to increase the yield of rice. One type of technology has given rise to a host of concerns and questions, namely GMOs. The significance of environment change and genetic safety has been recently recognized by the commercialization of GM crops. The scientific evidence concerning the environmental and health impacts of GMOs is still emerging, but so far there is no conclusive information on the definitive negative impacts of GMOs on health or the environment. Nevertheless, public perceptions about GMOs in food and agriculture are divided with a tendency toward avoiding GM food and products in many developed and developing countries. Also Korea is one of that country and is not allow the GMOs now. So I studied whether these GMOs are actually dangerous for environment and whether there are differences in cultivar characteristics such as germination test with TTC tetrazolium, germination test in frozen soil and gene-flow test with glufosinate and strip-bar test. These experiments will indicate that drought-tolerant GM rice may be used to detect genetic safety and evaluation standards in GM rice progeny. *Corresponding Author: Tel , kkm@knu.ac.kr 135

192 PD-10 Rahmatullah Jan, Kyung-Min Kim Chorismate mutase gene isolated from rice was cloned and functionally expressed in yeast. This gene encodes a bifunctional protein of 125 amino acids and KDa molecular mass. It possesses catalytic activity and catalyzed a very main step of converting an intermediate metabolite chorismate to prephenate and furthermore to phenylalanine and tyrosine which are the ultimate sources of secondary metabolites synthesized in shikimate pathway. Not only Phe and tyr are essential compounds for protein synthesis but also secondary metabolites have a predominant value in plant growth promotion and defense against environmental stresses. To functionally express the target gene were cloned in an episomal plasmid of RS series and prs42k was designed with PGK promoter and CYC1 terminator sites resistant to gentacine. This plasmid used as a shuttle vector i.e. multiply both in E.coli and yeast. In this experiment E. coli, DH5α and S. cerevisiae D452-2 strain were used for multiple copies of insert and functional expression respectively. Quantitative analysis of aromatic amino acid biosynthesis confirmed that expression of CM gene significantly increases phenylalanine 26% and tyrosine 24%. HPLC analysis revealed that expression of CM gene in transformed strain increases secondary metabolites than non-transformed strain and also demonstrated that the rate of secretion of secondary metabolites to liquid media was higher than that of accumulation inside the cell. Western blot analysis also shows that recombinant protein related to CM gene was significantly expressed in transformed strain as compare to wild type strain. Acknowledgement: This work was supported by a grant from the Next-Generation BioGreen 21 Program (No. PJ ), Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , kkm@knu.ac.kr PD-11 Leitao Pedro Isabel, Rahmatullah Jan, Kyung-Min Kim * Cultivated oat, Avena sativa L., is a self-pollinating,disomic, hexaploid (2n = 6x = 42) species. The genome of hexaploid Avena species consists of three basic subgenomes,referred to as the A, C, and D genomes, each of which contains seven pairs of chromosomes. The objective of this study is to construct the genetic map of cultivated hexaploid oat by EST-SSR markers and QTL analysis of major agronomic characters. Total RNA from both oats cultivars (seonyang and daeyang) were extracted from young leaves after 1 month of growing process using Trizol reagent. 100 mg of each leaf cultivars samples were well grind in LN2 after cutting using sterilized scissor and liquid nitrogen (-120 C). Measured the RNA concentration (seonyang: ng/μ L and ng/μ L and daeyang: ng/μ L and ng/μ L. RNA samples (10 μ L) from both cultivars were then utilized for cdna synthesis. From the samples checked in gel electrophoresis were excised the DNA fragment (cutting) with scalpel by gel extraction. Apart from this process another 100 oats cultivars were sowing and each DNA were extracted after 26 days of growing process according to Solgent Co.,Ltd instructions. *Corresponding Author: Tel , kkm@knu.ac.kr 136

193 PD-12 Sukyeung Lee 1*, Yu-Mi Choi, Do yoon Hyun, Myung-chul Lee, Sejong Oh, Onsook Hur, Hocheol Ko, and Gyu Taek Cho 1 One of most important global food trends is health. Oat is rich in protein and lipids compared to other grains, and contains a large amount of dietary fiber, β -glucan. Korean domestic consumption of oat, one of top 10 super food, is rapidly increasing due to the high nutritional value. However, the researches on evaluation of cultural and functional characteristics of oat genetic resources as breeding material has been insufficient because oat has been studied as feeds lather than food. Therefore, this study aims to provide information of agricultural traits, physicochemical property(crude proteins, lipids, fibers), and the most important nutritional ingredient, β -Glucan of 142 oat germplasms from Korea, China, and Japan which are maintained in National Agrobioversity Center(NAC) to be used as basic research as oat breeding. On morphological characteristics, Korean oats were averagely headed and matured earlier than Chinese and Japanese ones. Most of oat accessions were not suitable for double cropping with rice. But, 7 accessions including IT151107(Korea-origin) was matured before mid June which could be cultivated with paddy rice in Korean Southern region. IT166575(1.8g,Korea-origin) and IT128790(4.2g,China-origin) were the accessions having the lightest and the heaviest 100 seed weight, respectively. There were also some accessions having colorful outer glum as brown or even black(it162928, Japan-origin). Such resources having unique characteristics could be useful as breeding sources. The ranges of protein, lipids, fiber, and β -glucan contents of oat germplasms in this study were from 11.6% to 22.9%, from 1.4% to 12.0%, from 2.3% to 5.7%, and 0.80% to 2.69%, respectively. Chinese oats had averagely higher protein (17.1%) and β -glucan(4.1%) contents than Korean(15.9%, 3.7%) or Japanese ones(15.6%, 4.0%). Korean oats had averagely higher lipids(5.7%) and fiber(1.8%) contents than Chinese(4.9%, 1.3%) or Japanese(4.0%, 1.3%) ones. IT166594(China-origin), IT166584(China-origin), IT129802(China-origin), and IT151108(Korea-origin) showed the highest levels of protein, lipids, β -Glucan, and fiber content, respectively. These resources having high content of major nutrients can be useful breeding sources for functional oat food materials *Corresponding Author: Tel , reset00@korea.kr PD-13 Su-Jeong Kim *, Hwang-Bae Sohn, Su-Young Hong, Bon-Cheol Koo and Yul-Ho Kim Genetic resources of tartary buckwheat (Fagopyrum tataricum Gaertn.) preserve useful genetic variation, yet they remain untapped due to lack of genetic markers. In this study, we used high throughput next-generation sequencing (NGS) data in order to unravel the genetic resources for developing buckwheat as a highly functional food crop. Overall, 26 core resources of tartary buckwheat has been collected from six countries including China, India, and Nepal. A comparative genomic study has explored a large number of InDel (insertion/deletion) markers required for developing common platform. Bioinformatic analysis revealed 171,926 and 53,755 homo- and hetero-indels, respectively. Among them, 50 in silico polymorphic InDels from 26 accessions were selected by gel electrophoresis, which were converted as barcode types by comparing amplicon polymorphisms with the reference sequence. In order to make user-friendly common platform for genotype, phenotype and chemotype resources, we incorporated genotypic data with that phenotype and chemotype (rutin content) data of 26 buckwheat accessions. As a user friendly system, the homology between the accessions can be visualized in both one (1D) and two dimension (2D) as blocks. Our platform could be not only used in genetic research and breeding programs but also used for efficient resource management system in buckwheat. *Corresponding Author: Tel , sjkim30@korea.kr 137

194 PD-14 Yul-Ho Kim *, Su-Jeong Kim, Hwang-Bae Sohn, Su-Young Hong and Bon-Cheol Koo Tartary buckwheat possesses more interesting nutrient profiles, including higher levels of flavonoids compared to the common buckwheat. In this study, we report a draft genome assembly of a high-rutin tartary buckwheat (Fagopyrum tataricum), which included 43,771 protein-coding gene models captured in 526 million base-pairs (Mbps). The diploid genome showed a signature of a single whole genome duplication dated before its divergence from common buckwheat (F. esculentum) and after the divergence from Amaranthaceae family crop species. Comparative analyses identified an enrichment of transcription factors among Fagopyrum28 specific gene families. The genome of tartary buckwheat included higher copy numbers of genes encoding enzymes synthesizing precursors of rutin than those of common buckwheat and Amaranthaceae family crops. Notably, we identified an inverted tandem duplication of gene loci encoding paralogs of flavonol synthase 1 (FtFLS1) that showed flower-specific expression patterns in the tartary buckwheat. The inverted tandem duplication was also present in the co-linear region in the genome of grape, another species known for high flavonol content, but absent in genomes of common buckwheat and Amaranthaceae crops. The tartary and common buckwheat genomes included uniquely expanded additional copies of enzymes representing specialized metabolic profiles specific to each species. The tartary buckwheat genome has expanded gene families encoding nitrate and phosphate transporters as well as enzymes synthesizing phenylpropanoid and terpenoids, exemplifying an adaptive strategy that optimize growth with a buildup of defense molecules low in nitrogen and phosphorous in a nutrient-poor habitat. Our draft genome, the pan-genomes of buckwheat and the comparative analyses with other plant genomes provide insight and resources for studying the genomic basis of adaptive evolution specially related to flavonoids. *Corresponding Author: Tel , kimyuh77@korea.kr PD-15 B. Kim 1, C. Kim 2 and S. Kim 1* 1 2 A novel onion (Allium cepa L.) cytoplasm, cytotype Y, was found in a previous study. Cytotype Y contained unique stoichiometry of coxi and orf725, a candidate gene responsible for male-sterility induction in onions. A S 1 segregating population was produced from a single plant selected from PI Although male-fertility segregated in this population, the ratio significantly deviated from single-gene inheritance. However, genotypes of RF31446 marker perfectly linked to Ms locus controlling fertility restoration completely matched with male-fertility phenotypes, indicating that male-fertility restoration of male-sterility conferred by cytotype Y might be determined by the Ms locus. One plant derived from the S 1 population showed discrepancy between male-fertility phenotype and RF31446 genotype. Although the RF31446 genotype was homozygous recessive, reduced amount of pollen grains were observed in anthers. Many pollen grains of the unstable male-sterile plant were deformed. Analysis of 13 molecular markers flanking the Ms locus showed no crossover between the Ms locus and the RF31446 marker. Ten more unstable male-sterile plants were identified from open-pollinated progenies of the unstable male-sterile plant. Viable seeds were successfully produced from unstable male-sterile plants, indicating that pollen grains of the unstable male-sterile plants were partially viable. In addition, an umbel containing unstable male-fertile flowers was identified from one of maintainer lines, although both male and female organs might be sterile in these flowers. *Corresponding Author: Tel , dronion@jnu.ac.kr 138

195 PD-16 Mia Park 1, Gregory B. Tilton 2, Erin Bell Maize (Zea maysl.) is known as a highly genetically diverse species, which is reflected in the considerable natural variability in composition. The paper reviewed here provides an in-depth compositional analysis of a set of hybrids based on Nested Association Mapping (NAM) founder lines and landraces that were selected for their genetic diversity, and documents the variability in the levels of a large set of grain components. 25 inbred lines selected to represent genetic diversity in maize (NAM inbreds) and 24 inbred lines derived from a diverse collection of landraces were hybridized with B73, an inbred line that has a high-quality reference genome. These hybrids were planted in 2012 in a replicated trial at a single location in the United States. Components from the harvested grain were analyzed for proximates, fiber, minerals, amino acids, fatty acids, tocopherols, β -carotene, phytic acid, and raffinose. Results reported in the publication demonstrated that while composition segregated by group (ex. tropical vs. temperate varieties) extensive variation existed across all grain components assessed for both the NAM and landrace hybrids, reflecting the underlying genetic diversity of these lines. The results from these hybrids are important because they provide a first survey of grain composition in hybrids from two important genetic resources, the NAM founder lines and landraces. *Corresponding Author: Tel , mia.park@monsanto.com PD-17 Jiyoung Kim, Doh-Won Yun, Soo-In Sohn, Gang-Seob Lee, SongJae Ok, Ancheol Chang, Sung-Dug Oh * Genetically modified (GM) crops have been developed worldwide through the recombinant DNA technology and commercialized by global agricultural companies. Until now, GM crops have not been cultivated commercially in Korea. Commercialization of GM crops requires a compulsory assessment of environmental risk associated with the release of GM crops. This study was conducted to evaluate the frequency of pollen mediated gene flow from Bt transgenic rice (Agb0101) to japonica non-gm rice (Nakdongbyeo), indica non-gm rice (IR36), and weedy rice (R55). A total of 729,917, 596,318 and 230,635 seeds were collected from Nakdongbyeo, IR36, and R55, respectively, which were planted around Agb0101. Selection of the hybrids was determined by repeated spraying of herbicide and Cry1Ac1 immunostrip assay. Finally, the hybrids were confirmed by PCR analysis using specific primer. The hybrids were found in all non-gm rice and out-crossing ranged from % at IR36 to % at Nakdongbyeo. All of hybrids were located within 1.2 m distance from the Agb0101 rice plot. The meteorological elements including rainfall and temperature during rice flowering time were found to be important factors to determine rice out-crossing rate. Consideration should be taken for many factors like the meteorological elements of field and physiological condition of crop to set up the safety management guideline to prevention of GM crops gene flow. *Corresponding Author: Tel , ohbaboh@korea.kr 139

196 PD-18 Eun-Seok Oh and Man-Ho Oh Plants adjust to changing conditions and make decisions based on sensing substances such as water, light, external nutrients and internal nutrient reserves. Plants also utilize long distance signaling mechanisms for a variety of biological processes. Environmental responses can also involve peptides and Leucine-Rich Repeat Receptor-Like Kinases (LRR-RLKs). In Arabidopsis, CEP1 peptides, unrelated to CLE peptides, are synthesized and move through the xylem to shoots, where they are perceived by the two LRR-RLKs CEPR1 and CEPR2, resulting in long-range stimulation of root growth in nitrogen rich conditions. In addition, cytoplasmic domains of Flag-CEPR1 also autophosphorylate on threonine and tyrosine residues and thus are dual-specificity kinases. Primary root length of wild type seedlings was inhibited by cep1 peptide treatment under normal growth condition. Comparison of WT and cepr1/2 double knock-out mutants after treatment of cep1 peptide in terms of primary root length shown that cepr1/2 double mutants was not response to cep1 peptide treatment compare to root length of WT seedlings. As another interesting results, we monitored expression pattern of genes, possibly related to regulation of sugar/nitrate balance under cep1 peptide treatment in vivo. Among genes investigated, transcripts level of PPDK, PPC2, CYP79F1 and NRT2.1 are increased with cep1-dependent manner. Theseresult demonstrated that cep1 is interconnected to nitrate uptake and root growth in Arabidopsis. *Corresponding Author: Tel , manhooh@cnu.ac.kr PD-19 Sang-Woo Choi *, Jun-Hyun Park, Jong-Il Chung 1 1 Soybean [Glycine max(l.) Merr.] seed is an important dietary source of protein, oil, carbohydrates, isoflavones, and other nutrients for humans and animals. But, antinutritional factors in the raw mature soybean are exist. Kunitz trypsin inhibitor (KTI) protein and stachyose are main antinutritional factors in soybean seed. The genetic removal of the antinutritional factors will improve the nutritional value of black soybean seed. The objective of this research was to breed a new strains with the traits of lacking of KTI protein and low content of stachyose. Presence or absence of KTI protein was detected based on Western Blot technique. Content of stachyose in mature seed was detected by HPLC. Total five new strains (603-1, 603-1brown, 603-2, 625, and 694) with KTI protein free and low content of stachyose were developed. Four strains(603-1, 603-2, 625, and 694) have yellow seed coat and hilum. One strain(603-1brown) has brown seed coat and white hilum. Plant height of strain was 65cm and 100-seed weight was 29.2g. Plant height of strain was 66cm and 100-seed weight was 26.2g. Plant height of 625 strain was 64cm and 100-seed weight was 27.1g. Five strains selected in this research will be used to improve new cultivar with KTI protein free, and low content of stachyose. *Corresponding Author: Tel , jongil@gnu.ac.kr 140

197 PD-20 Sang-Woo Choi *, Jun-Hyun Park, Jong-Il Chung 1 1 Soybean [Glycine max (L.) Merr.] seeds contain protein, carbohydrate, oil, vitamins, minerals, and many functional components. Anthocyanins from black soybean seed coat are known to have many pharmaceutical effect. Soybean cultivar with large seed size and black seed coat is needed by soybean farmer. But, antinutritional factors in the raw mature soybean are exist. Soybean Kunitz trypsin inhibitor and lipoxygenase proteins are well known as antinutritional factors. Lipoxygenase is responsible for the beany flavor and Kunitz trypsin inhibitor (KTI) protein is responsible for the inferior nutritional quality of unheated or incompletely heated soybean meal. The objective of this research was to breed a new strains with black seed coat, large seed size, and lacking of lipoxygenase and KTI protein. A few parents were used. Presence or absence of KTI protein was detected based on Western Blot technique and absence of lipoxygenase protein were selected by SDS-PAGE analysis. New strain with black seed coat, large seed size, and lacking of lipoxygenase and KTI protein was developed. Plant height of new strain was 48cm and 100-seed weight was 36.0g. Acknowledgement: This work was supported by the IPET(Project No ) *Corresponding Author: Tel , jongil@gnu.ac.kr PD-21 Soon-Jae Kwon 1*, Min-Kyu Lee 1,2, Dong-Gun Kim 1,3, Jung Min Kim 1,2, Min Jeong Hong 1, Bo Mi Nam 1, Chang Hyun Jin 1, Hong-Il Choi 1, Bo-Keun Ha 2, Jin-Baek Kim Perilla frutescens var. crispa (Labiatae), which is known as Cha-Jo-Ki in Korea, Zi-Su-Ye in China, and Shiso in Japan, is widely cultivating in East Asia. It has been used as a medicinal herb and oil production. A new radiation mutant cultivar P. frutescens var. crispa (vs. Antisperill) was developed recently, which has a 25-fold higher content of isoegomaketone (IK) than wild type. The IK of perilla has an effect for arthritis. For determining and evaluation of IK related genes between Antisperill and wild type, we performed RNA-seq with three growth stages in both perilla cultivars. In total, 132,943 transcripts and 36,995 representative transcripts were identified in three repetitive mrnas. Of the 36,995 representative transcripts, 25,510 (69.96%) sequences had similarity with the GO, KOG, and KEGG amino acid sequences. We identified 65, 131, and 230 differentially expressed genes (DEGs) between the mutant and wild type in 70, 94, and 122 days after sowing, respectively. With the exception of redundancy, a clustering analysis was performed using 362 unigenes. Among these genes, 110 homologs of P. frutescens terpenoid biosynthesis pathway related genes were identified and seven genes were related to monoterpenoid biosynthesis, which is thought to be the pathway of IK. However, the correlation between the seven candidate genes and IK contents depending on the growth stage should be compared in a further study. *Corresponding Author: Tel , soonjaekwon@kaeri.re.kr 141

198 PD-22 Kesavan Markkandan 1, Jinuk Bhak 1, Dong Woo Lee 1* 1 In genomics era, genetic assisted breeding is more effective tool, which improves the crops cultivation from different environmental factors and prevent from revenue loss. Cross breeding of different phonotypes is resulted with new cultivars by the DNA recombination. Those, Cultivars are evolved with low levels of genetic diversity in specific genome loci with high recombination rate. Those regions are filled with high dense of nucleotide variations, and these regions are called as Variation Blocks (VB). These variations blocks are highly interlinked with the phenotypes. Here, we established barcode system approach based on insertions/deletions (InDel) markers of crop cultivars. The VBs were mined by analyzing whole genome data cultivars followed by putative InDels in the VB regions were identified for the development variation block browse. In addition, the changing of the VBs in a chromosomal level can be quickly identified due to investigation of the reshuffling pattern between cultivars. This browser is more useful for detecting the recombinant loci and trait associated marker, which are more effective for agricultural traits and to identify agronomical important genes, which could use for gene cloning applications. Moreover, VB browser facilitate genome wide VB visualizations using genome browsers. VB browsers is publicly available as Cropsquare ( *Corresponding Author: Tel , dongwoo.lee@theragenetex.com PD-23 Jaihyunk Ryu 1,3, Woon Ji Kim 1, Kyung-Won Kang 2, Sang Hoon Kim 3, Si-Yong Kang 3, Jeong-Hee Lee 4, Bo-Keun Ha 1* The genus Dendrobium is the third largest in the Orchidaceae, comprising approximately 1,190 species worldwide. Dendrobium plants are among the most prevalent orchids for horticultural industries and about 40 Dendrobium species have been used in traditional medicine from Eastern Asia including Korea, China, and Japan. Recently, new Dendrobium mutant lines showing several distinctive characteristics for stem, leaf and flower colors and/or dwarf have been developed by mutation breeding technology in Korea. In this study, we evaluated genetic variations in Dendrobium genotypes using single nucleotide polymorphism (SNP) markers generated from genotyping-by-sequencing (GBS) analysis and to develop a Kompetitive Allele Specific PCR (KASP) assay for a set of SNPs. GBS analysis was conducted with 18 Dendrobium genotypes, consisting of seven mutant lines and elven commercials cultivars. A total of 517,660 high-quality union loci were detected, which contained 443,305 homozygous SNPs and 74,355 heterozygous SNPs. A set of 37,721 filtered SNPs was used to perform a phylogenetic analysis, which showed that there were clear differences among the Dendrobium genotypes based on their species. The ten KASP assays were successfully developed to distinguish among the Dendrobium genotypes. This study demonstrated that the KASP method is an economically efficient tool for mutant screening in Dendrobium breeding programs. *Corresponding Author: Tel , bkha@jnu.ac.kr 142

199 PD-24 Jaihyunk Ryu 1,3, Woon Ji Kim 1, Kang-Seop Lee 2, Han-Jig Jo 2, Ee-Youb Kim 2, Si-Yong Kang 3, Jeong-Hee Lee 4, Bo-Keun Ha 1* Rubus is an economically important berry crops because of its potential health benefits on human. Recently, several Rubus mutant genotypes showed improved agronomic traits such as thorneless, higher fruit yields and disease resistance obtained from gamma-ray. This study investigated genetic diversity and variations in Rubus mutant genotypes using single nucleotide polymorphism (SNP) markers generated from genotyping-by-sequencing (GBS) analysis and to develop a Kompetitive Allele Specific PCR (KASP) assay for a set of SNPs in Rubus mutant genotypes. A GBS library of 14 Rubus genotypes, consisting of seven hybrid mutant lines, four blackberry mutant lines, and three original genotypes, were sequenced on the Illumina Hiseq2000 platform. A total of 50,831,040 reads of clean data were generated, and the trimmed length ranged from 116,380,840 to 509,806,521 bp, with an average of 228,087,333 bp per line. A total of 19,634 high-quality SNPs were detected, which contained 11,328 homozygous SNPs and 8,306 heterozygous SNPs. A set of 1,504 SNPs was used to perform a phylogenetic analysis, which showed that there were clear differences among the Rubus genotypes based on their origin. The genetic diversity matrix (GDM) revealed variability among the mutant genotypes ranging from to The six KASP assays were successfully developed to distinguish among the original genotypes and mutant genotypes in Rubus hybrid. This study demonstrated that the SNP and KASP method is an economically efficient tool for mutant screening in Rubus breeding programs. *Corresponding Author: Tel , bkha@jnu.ac.kr PD-25 고세리 1*, 장지영 1, 이주영 1, 최규환 1 1 시스템합성농생명공학사업단은 1-2 단계차세대바이오그린 21 사업을통해고부가가치유용물질생산용슈퍼농생물체개발및산업화를위한기초 원천기술개발연구과제들을수행하고, 우수한논문및산업재산권등의성과를거두었으나기술이전등을통한연구성과확산및실용화추진노력이필요한실정이다. 본연구에서는사업단수행과제로부터도출된총 390 건의산업재산권성과를분석, 국내외기업으로의기술이전촉진및성과확산이가능한유망핵심기술들을선발하고, 최신기술동향및시장동향조사를통한타겟시장및수요기업발굴과기업맞춤형개별기술, 또는관련기술의기술패키징을통한효율적성과확산추진체계를구축하고자한다. 시스템합성농생명공학사업단선행과제도출대량산업재산권은 SMART3 특허등급평가로분석, 권리성, 기술성, 활용성분야의특허등급을종합적으로평가하여실용화유망기술을객관적지표로 1 차선발하였다, 그결과, 2018 년 1 월현재국내등록유지특허 247 건중총점등급 BBB 등급이상기술은총 102 건 (42%) 이었고, 기술분류체계에따라분석하면, 분자육종소재개발연구기술이총 65 건, 기능성물질생산시스템 44 건, 기능성소재응용기술 77 건등으로전체특허의약 77% 이었고, 미생물제재등검출 / 진단기술등기타기술도약 25 건으로파악되었다. 따라서, 사업단의중점추진목표와부합하는고기능성산업용소재생산을위한미생물과식물시스템개발기술을중심으로국내화장품, 의약학품, 건강기능성식품산업분야로의성과확산이유망한기술을기술이전대상으로삼고, 온 - 오프라인기술마케팅을위한 SMK 제작및기술이전을위한연구자 - 사업단 - 수요기업간의네트워크연계를구축할예정이다. 또한, 3 단계수행과제중 1-2 단계연계또는신규로선발되어수행되는과제에서창출될미래성과들을기술사업화에활용하기위한전략적지식재산권포트폴리오구축등을지원할것이다. * 교신저자 : Tel , skohtn@naver.com 143

200 PD-26 Min-Kyu Lee 1,2, Dong-Gun Kim 1,3, Jung Min Kim 1,2, Bo-Keun Ha 2, Min Jeong Hong 1, Jin-Baek Kim 1, Si-Yong Kang 1, and Soon-Jae Kwon 1* This study was to survey the radiosensitivity of gamma-ray on seed of faba bean as well as identify genetic variation and mutation frequency among the mutants by TRAP markers. Ten elite faba bean lines were irradiated with gamma rays ( Gy), and the germination and survival rate, as well as representative morphological traits were measured. The extent of DNA damage was investigated using comet assay, and TRAP markers were used for evaluation of genetic variation, genetic diversity and mutation frequencies. The germination rate decreased at doses greater than 100 Gy. The survival rate and morphological traits decreased as the radiation dose increased. The comet assay revealed that increasing doses of gamma rays decreased head DNA levels. The phylogenetic and PCA indicated that the 555 individuals belonged to eight major groups. Genetic variations between the control and mutants were limited to the intra-group. The mutation frequencies were related gamma dosages in each mutant line. The optimal gamma dosage were revealed by 100~150 Gy. The TRAP markers were distinct separated mutant lines and showed association between mutation frequency and gamma dosages. This study will be very useful for faba bean mutation breeding and may be applicable to other crops breeding strategy. *Corresponding Author: Tel , soonjaekwon@kaeri.re.kr PD-27 Joung Sug Kim 1, Songhwa Chae 1, Kyong Mi Jun 2, Gang-Seob Lee 3, Dong-Eun Kim 4, Jong-Joo Cheong 5, Sang Ik Song 1, Baek Hie Nahm 1,2 and Yeon-Ki Kim 1* Plants have evolved strategies to cope with drought stress by maximizing physiological capacity and adjusting developmental processes such as flowering time. The WOX13 orthologous group is the most conserved among the clade of WOX homeodomain-containing proteins and is found to function in both drought stress and flower development. In this study, we isolated and characterized OsWOX13 from rice. OsWOX13 was regulated spatially in vegetative organs but temporally in flowers and seeds. Overexpression of OsWOX13 (OsWOX13-ov) in rice under the rab21 promoter resulted in drought resistance and early flowering by 7-10 days. Screening of gene expression profiles in mature leaf and panicles of OsWOX13-ov showed a broad spectrum of effects on biological processes, such as abiotic and biotic stresses, exerting a cross-talk between responses. Protein binding microarray and electrophoretic mobility shift assay analyses supported ATTGATTG as the putative cis-element binding of OsWOX13. OsDREB1A and OsDREB1F, drought stress response transcription factors, contain ATTGATTG motif(s) in their promoters and are preferentially expressed in OsWOX13-ov. In addition, Heading date 3a and OsMADS14, regulators in the flowering pathway and development, were enhanced in OsWOX13-ov. These results suggest that OsWOX13 mediates the stress response and early flowering and, thus, may be a regulator of genes involved in drought escape. *Corresponding Author: Tel , kim750a11@gmail.com 144

201 PD-28 Yun-Joo Kang 1, Bo-Mi Lee 1, Jae-Eun Oh 1, Moon Nam 1, Ki-Won Oh 2, Myoung-Hee Lee 2, Tae-Ho Kim 3, Sung-Hwan Jo 1*, and Jeong-Hee Lee 1* Perilla seeds are good source of polyunsaturated fatty acids such as DHA and EPA, ALA. Accumulation of oil contents in perilla are closely related to flowering time (FT). Three traits which associated with flowering time, days to visible flower bud (DtoFB) and days to flowering (DtoF), days to maturity (DtoM) are positively correlated (68% to 86%). To identify FT QTLs, F2 populations were developed by an interspecific cross between P. citriodora and P. hirtella. Through genotyping-by-sequencing (GBS) of 96 F2 populations, a total of 9,607 SNP markers were identified, of which 2,518 markers were grouped into 10 linkage groups spanning cm with an average distance of 0.56 cm. Using this map, QTL analysis was performed and two DtoFB QTLs, three DtoF QTLs, and one DtoM QTL were detected. In addition, orthologue gene analysis with known genes involved in the regulation of FT among the crop species is underway. These result might be useful tools for perilla breeding to develope new perilla varieties with high content of polyunsaturated fatty acid. Acknowledgement: This work was supported by a grant from the National Agricultural Genome Project (No. PJ ), Rural Development Administration, Republic of Korea. *Corresponding Author: shjo@seeders.co.kr, jhlee@seeders.co.kr PD-29 Me-Sun Kim 1, Jae-Young Song 1, Sothea Ouk 1, Franz M. Nogoy 1, Marjohn Nino 1, Kwon-Kyoo Kang 2, Sun-Hee Woo 1, Yong-Gu Cho 1* 1 2 Molecular markers are useful tools for evaluating genetic diversity and determining cultivar identity. In this study, we examined the genetic distance among Korean rice varieties using allele frequencies and a genetic diversity analysis with Simple Sequence Repeats (SSRs) markers. The analysis of the genetic diversity and genetic relationships of 243 Korean rice varieties was varied out using 20 SSRs markers. A total of 268 alleles were detected, ranging from 6 to 32, with an average of alleles per locus, and averages of gene diversity (GD) of Seven SSR markers were selected as key markers for discrimination among Korean rice varieties. As the results, 243 varieties (100%) were discriminated by using acrylamide gel and fragment analyzer-based markers. In conclusion, this study provides useful basic data that can be utilized in Korean rice varieties breeding and development. In addition, we will have to manage and conserve as a valuable genetic resource, without losing diversity of Korean rice varieties. Acknowledgement: This research was supported by the Ministry of Science, ICT and Future Planning and the Golden Seed Project ( WT211), MAFRA, Republic of Korea. *Corresponding Author: Tel , ygcho@cbnu.ac.kr 145

202 PD-30 Me-Sun Kim 1, Sothea Ouk 1, Le Van Trang 1, Franz M. Nogoy 1, Marjohn Nino 1, Ju-Young Yang 1, Kwon-Kyoo Kang 2, Yong-Gu Cho 1* 1 2 Leaf traits are always key issue to investigate in plant research. In this study, we conducted to investigate a morphological trait in 294 rice accessions including Korean breeding lines. We also carried out a genome-wide association study (GWAS) to detect significant single nucleotide polymorphism markers and candidate genes affecting major agronomic traits. A Manhattan plot analysis of GWAS using morphological traits showed that phenotypic and statistical significance was associated with a chromosome in each group. The significance of SNPs that were detected in this study was investigated by comparing them with those found previously studied QTL regions related to agronomic traits. As a result, SNP (S ), which is significant with regard to leaf angle, was located in the known QTL regions. To observe gene mutations related to leaf angle in a candidate gene, Os08g31950, its sequences were compared with sequences in previously selected rice varieties. In Os08g31950, a single nucleotide mutation occurred in one region. To compare relative RNA expression levels of candidate gene Os08g31950, obtained from GWAS analysis of 294 rice accessions and related to lateral leaf angle, we investigated relative levels by selecting 10 erect leaf-angle varieties and 10 horizontal leaf-angle varieties and examining real-time PCR. In Os08g31950, a high level of expression and various expression patterns were observed in all tissues. Also, Os08g31950 showed higher expression levels in the erect leaf-angle variety group and higher expression rates in the leaf than in the root. The candidate gene detected through GWAS would be useful in the development of new rice varieties with improved yield potential through future molecular breeding Acknowledgement: This work was supported by a grant from the Next-Generation BioGreen 21 Program (PJ ), RDA, Republic of Korea. *Corresponding Author: Tel , ygcho@cbnu.ac.kr PD-31 김용호 1*, 최도진 1, 이주원 1, 마무현 1, 이유정 1, 조민식 1, 이주희 1, 김홍식 이소플라본은콩에함유되어있는주요한기능성성분으로 phyto-hormone 역할을한다. 그동안이소플라본은콩종실, 콩잎등에서함량분석실험이수행되었을뿐조직배양에서의응용에관한연구는없었다. 따라서콩 callus 에서의이소플라본축적을확인하고자본연구를수행하였다. 콩장려품종대풍 2 호의종자에서배아를적출하고소독한후 MS 배지에치상하여 callus 를유도하였다. Callus 유도는첨가된식물생장조절제에따라반응이달랐으며, 생장조절제는또한 callus 의계대배양기간에도영향을미쳤다. 배아를치상한 1 개월후 callus 를수확하여 HPLC 를사용하여이소플라본함량을분석하였다. 전체이소플라본함량은여러가지처리에따라 1,280 2,140ug/g 변이를보였다. 또한총 12 가지이소플라본종류중 malonylglucosides 의함량이전체함량의 80% 이상을차지하여 malonylglucosides 가콩의주된이소플라본임을알수있었다. 한편, 식물생장조절제처리에따라이소플라본함량이달라짐을확인할수있었다. 이와같은결과를볼때추후조직배양을통하여이소플라본을대량생산할수있으리라판단된다. * 교신저자 : Tel , yohokim@sch.ac.kr 146

203 PD-32 김선림 1*, 김미정 2, 정건호 1, 이유영 1, 이진석 1, 손범영 1, 김정태 1, 배환희 1, 고영삼 1, 백성범 찰옥수수의출사후일수경과에따른종실의지방산조성과 phytosterol 의함량변화를구명하여고품질풋옥수수생산을위한기초자료로활용하고자본연구를수행하였다. 본시험에사용된찰옥수수는국립식량과학원에서육성된일미찰과흑진주찰 2 품종이었고, 출사후일수경과에따라 5 회 (21, 24, 27, 30, 33 일 ) 시료를채취하여백립중, 지방산조성및 phytosterol 함량을검토하였다. Phytosterol 의분석을위해서는시료를 saponification 처리후비누화반응이유도되지않은불검화물 (unsaponifiables) 을취하여 BSA[N,O-bis(trimethylsilyl)acetamide], pyridine, hexane 을순차적으로가하여 TMS 유도체화하였고, HP-5ms capillary column 으로분석을하였다. 본시험을수행하여얻어진결과를요약하면다음과같다. 출사후일수가경과할수록찰옥수수종실의조지방함량은지속적으로증가를하였고, 흑진주찰은일미찰보다조지방함량이다소높았다. 출사후일수의경과에따른지방산의조성비는뚜렷한변화를보였는데, palmitic(c16:0) 및 linoleic acid(c18:2) 는점차감소되었으나, oleic acid(c18:1) 는증가되는것으로나타났고, 흑진주찰은일미찰에비해불포화지방산 (USFA) 의조성비가다소높은것으로나타났다. 출사후일수가경과할수록총 phytosterol 의함량은증가되었으나품종간총함량의차이는보이지않았다. 그러나 phytosterol 의조성비는품종간차이를보였는데, 일미찰은 β-sitosterol > stigmasterol > campesterol 순으로조성비가높았으나, 흑진주찰은 β-sitosterol > campesterol > stigmasterol 순으로조성비가높았으며, 성분별로볼때 campesterol 및 ß-sitosterol 함량은품종간차이가없었으나 stigmasterol 은흑진주찰이일미찰에비해함량이높은것으로나타났다. 출사후 100 립중증가비율에따른조지방, USFA 및 phytosterol 의증가비율의관계를검토한결과 100 립중이증가할수록조지방및 phytosterol 함량도증가되었으나, USFA 의조성비는변화가적었다. * 교신저자 : Tel , kimsl@korea.kr PD-33 최재근 1*, 박종열 1, 장은하 2, 류시환 1, 박기진 1, 남궁민 1, 윤석원 1, 용우식 1, 최준근 국내팝콘옥수수는대부분수입에의존하고있으며, 매년 10,000 톤정도가수입되어극장및놀이공원등에서소비가되고있다. 옥수수연구소에서는수입산팝콘원료를대체하기위하여 1997 년부터튀김옥수수연구를시작하여현재오륜팝콘, 지팝콘, 오륜 2 호를개발하여국내재배농가에보급하고있다. 하지만수입산에비해높은생산가격으로수요가확대되지못하고국내산을선호하는소비자층을중심으로소비가되며원료공급을위한재배단지가전국적으로 20ha 정도조성되어있다. 국내산품종의품질을높이기위해 2017 년부터지역특화과제로수확후관리기술개발을위해저장온도및저장방법등표준화기술을개발하여국내산팝콘품질을수입산과대등하게높여나가고있다. 국산품종의소비를높이기위해서는생산비를낮추고수량이높은품종을개발하고, 재배가쉬운품종을개발하다면팝콘원료의수입을대체할수있을것이다. 따라서본연구는튀김부피및수량성이높은품종을개발하고자수행하였다. 기찬팝콘 은튀김용모집단에서분리된 GP6 를모본, GP3 를부본으로하여개발하였으며, 수량성은 오륜팝콘 대비 6% 증수한 489kg/10a 로수량이높으며, 도복지수는 1 로 오륜팝콘 의 3 보다낮아내재해성도강하며, 100 립중은 15.6g 으로 오륜팝콘 16.2g 보다소립인특성을갖고있다. 튀김부피는알곡수분이 11.0~12.0% 일때 기찬팝콘 이 33.7 cm3 /g 로 오륜팝콘 의 31.1 cm3 /g 배보다높아가공용으로도적합한특성을가지고있어앞으로농가에보급하여재배단지를확대하면수입산을대체할품종으로기대한다. 사사 : 본연구는농촌진흥청지역특화기술개발연구사업 (PJ ) 의지원에의해이루어진것임 * 교신저자 : Tel , jaekeun@korea.kr 147

204 PD-34 Junki Lee 1, Hae Rim Park 1, So Yeon Kim 1, JongSung Lim 1, Green Jhang 1, Hyang Sook Chun 2, Kisung Kwon 3, Youngho Koh 3, Tae Sun Kang 3 and Gyoungju Nah 1* Adenophora triphylla and Codonopsis lanceolata have been used as a valuable medicinal sources and food in East Asia. However, both plants are difficult to be distinguished due to its similarity in morphology. The chloroplast (cp) genomes of both pharmaceutical plants were revealed and analyzed to investigate phylogenetic relationships and discover potential molecular markers for its authentication. We performed phylogenetic analysis indicating considerable distance between A. triphylla and C. lanceolata. In this analysis, the potential DNA markers based on the cp genome sequences were demonstrated. We identified potential DNA markers that carry Insertion/Deletion (InDels) that are able to identify these two species around genic-intergenic regions of ycf2, ndhb, rps7, and ycf1 loci. The cp genomes identified in this study would serve as useful tools for fundamental molecular understanding and future authentication of Adenophora and Codonopsis species. This research was supported by a grant (17162MFDS065) from Ministry of Food and Drug Safety, Korea, in Acknowledgement: We highly appreciate Plants Research Center at Hantaek Botanical Garden, Korea, for providing us plant materials. *Corresponding Author: Tel , gnah.nicem@snu.ac.kr PD-35 Junki Lee 1, So Yeon Kim 1, JongSung Lim 1, So Yeon Kang 1, Hyang Sook Chun 2, Kisung Kwon 3, Youngho Koh 3, Tae Sun Kang 3 and Gyoungju Nah 1* Chloroplast (cp) genome sequence has been served as a valuable source for understanding evolutionary history and developing molecular markers. The cp genomes of Hemerocallis fulva (medicinal plant)and Veratrum japonicum (inedible toxic plant) were revealed and analyzed to investigate phylogenetic relationships and discover potential molecular markers for its authentication. Phylogenetic analysis was indicated considerable distance between H. fulva and V. japonicum. We developed the potential DNA markers based on the cp genome sequences in this research. The five potential DNA markers including Insertion/Deletion (InDels) could be able to discriminate these two species around genic-intergenic regions of psba, psbl, atpa, psbz, psbm, psbd, and rps14 loci. The cp genomes identified in this study would help as useful tools for fundamental molecular understanding and future authentication of Hemerocallis and Veratrum species. This research was supported by a grant (17162MFDS065) from Ministry of Food and Drug Safety, Korea, in Acknowledgement: We highly appreciate Plants Research Center at Hantaek Botanical Garden, Korea, for providing us plant materials. *Corresponding Author: Tel , gnah.nicem@snu.ac.kr 148

205 PD-36 Jung Min Kim 1,2, Jaihyunk Ryu 2, Min-Kyu Lee 1,2, Dong-Gun Kim 1,3, Min Jeong Hong 1, Jin-Baek Kim 1, Si-Yong Kang 1, Bo-Keun Ha 2, Joon-Woo Ahn 1, Soon-JaeK won 1* Hibiscus belong to Malvaceae family consist of about 300 species such as H. cannabinus, H. sabdariffa, and H. rosa sinensis. However, the limitation of molecular markers have brought about low genetic diversity and relationship of Hibiscus germplasm resources. This study showed that we screened and evaluated transferability of 102 EST-SSR markers derived from kenaf to other 18 hibiscus species. One-hundred and one EST-SSR markers were successfully amplified. Among them, 100 markers showed polymorphism to 94 genetic resources/cultivars in the hibiscus genus. As a result, transferability rates varied from 82.35% (H. trionum) to 98.04% (H. ponticus) and the average revealed 89.02%. A total of 827 alleles were generated from the use of 101 EST-SSR markers, and the number of alleles ranged from 1 (RBRC_Hc_ES_73) to 16 (RBRC_Hc_ES_80) per locus and the average was 8.6. The PIC values ranged from 0 (RBRC_Hc_ES_73) to 0.86 (RBRC_Hc_ES_95), and the average was Moreover, we identified the genetic relationship among 18 hibiscus species. According to UPGMA clustering and a PCoA analysis, 18 hibiscus species were classified into three clusters. Cluster Ⅰ contained one species (H. acetosella), cluster Ⅱ included two species (H. sabdariffa and H. radiates), and the remaining 15 species were clustered in Cluster Ⅲ. The population structure with 94 genetic resources/cultivars was divided into three groups as well. In this study will be useful resources to evaluate genetic diversity and population structure of unclear germplasm among the hibiscus genus. *Corresponding Author: Tel.: , soonjaekwon@kaeri.re.kr PD-37 Jin-hyuk Kim 1, Junki Lee 1, Eun-Kyoung Kim 1, Minjung Kim 1, Sanghee Um 1, Soon-Sung Choi 1, Hwa-Young An 1, Hyang Sook Chun 2 and Gyoungju Nah 1* 1 2 The chloroplast plays a crucial role in maintaining life on Earth. Chloroplast genomes from a variety of land plants has enhanced our understanding of intracellular gene transfer, chloroplast biology, conservation, evolutionary history, diversity, and the genetic basis by which chloroplast transgenes can be engineered to enhance plant agronomic traits. The cp genomes of Kalopanax septemlobus and Zanthoxylum ailanthoides were analyzed to investigate phylogenetic relationships and to discover potential molecular markers for their recognition. We were able to distinguish these two species around the genic-intergenic regions with a potential DNA marker developed based on the sequence of chloroplast genomes. The cp genomes identified in this study would serve as useful tools for fundamental molecular understanding and future authentication of Kalopanax and Zanthoxylum species. This research was supported by a grant (17162MFDS065) from Ministry of Food and Drug Safety, Korea, in Acknowledgement: We highly appreciate Medicinal Herb Garden at College of Pharmacy, Seoul National University, Korea, for providing us plant materials. *Corresponding Author: Tel , gjnah@snu.ac.kr 149

206 PD-38 손재한 1*, 강천식 1, 최창현 1, 김양길 1, 박종호 1, 박태일 1, 윤영미 1, 정영근 1, 김경호 2, 이점호 밀의단백질함량과조성은빵, 면과같은밀의가공에매우큰영향을미친다. 밀의단백질함량은유전적, 환경적인영향을받지만, 유전적요인이큰것으로알려져있다. 빵용특성을갖는밀은 5+10 단백질조성을기본적으로갖고있어야하며, 최근 7 번단백질이밀의단백질함량을높이는것으로알려져있다. 특히 Bx7 OE 유전자를갖는밀의단백질함량이높아지는연구결과들이보고되고있다. 따라서본연구는밀의가공특성에맞는밀품종개발에유용하게사용할수있는자원개발을위해국내뿐만아니라북한, 몽골등외국자원을포함하고있는유전자원에대하여 Bx7 OE 유전자와관련된분자마커를평가하였다. 608 개자원평가결과를통해 7 개자원에서 Bx7 유전자가과발현하는것을확인하였다. 7 개계통은북한, 일본등여러지역에서수집된자원들이포함되어있었다. 이들계통의평균단백질함량은평균 12.5% 로확인되었으며, 특히 7 번단백질의함량이뚜렷하게높아지는것을확인하였다. 앞으로실제종자를이용하여단백질, 회분, 글루텐, 침전가와같은밀의품질특성과유전자간의상호관계를밝혀내고, 뛰어난자원을선발할수있는표지인자를개발한다면밀육종프로그램에매우유용하게이용될수있을것으로생각된다. * 교신저자 : Tel , pathfinder1@korea.kr PD-39 Dong-Gun Kim 1,2, Young Eun Jang 1, Min-Kyu Lee 1, Jung Min Kim 1, Min Jeong Hong 1, Jin-Baek Kim 1, Si-Yong Kang 1, Chang-Hyu Bae 2, Joon-Woo Ahn 1, Soon-Jae Kwon 1* 1 2 Soybean [Glycine max (L.)] is the most widely grown grain legumes in the world, which is widely used as the major sources of vegetable oils and plant proteins. Despite the economic importance of soybeans, there have been no TRAP marker system studies on genetic relationships between/among mutant lines. To develop a strategy of Mutant Diversity Pool (MDP) conservation, a study on the genetic diversity of 210 soybean mutant lines (8 cultivars and 202 mutants) was performed through a TRAP analysis. TRAP was conducted using sixteen primer combinations. The highest polymorphism level (86.96%) was obtained using a MIR157B + Sa4, whereas the lowest polymorphism level (31.03%) was obtained using a B14G15B + Ga3. Phylogenetic and principal component analysis (PCA) analyses indicated that the 210 lines belong to five groups based on the 16 combination TRAP markers. AMOVA showed 21.0% and 79.0% variations among and within the population, respectively. Overall, the genetic similarity of each cultivar and its mutants were higher than within other mutant populations. Our results suggest that the TRAP marker system may be useful for assessing the genetic diversity among soybean mutants and help to improve our knowledge of soybean mutation breeding. *Corresponding Author: Tel.: , soonjaekwon@kaeri.re.kr 150

207 PD-40 Kunyan Zou 1, Daewoong Lee 1, Nayoung Kwon 1, Tae-Hwan Jun 1* 1 Peanut is an allotetraploid because the single recent polyploidization caused significant decrease in genetic diversity. Development of SNP-based markers such as CAPS that for PCR amplification of DNA restriction enzyme analysis are widely applied in next-generation sequencing (NGS). This study aimed 1) to evaluate the availability of SNP markers and 2) to identify the peanut genetic diversity and structure populations using molecular markers and 96 peanut accessions from Peru, China, Argentina, Brazil, and Korea for data analyses. A total of 30 CAPS markers from 13 different chromosomes were selected, and twenty-eight of the CAPSs were in intergenic and two CAPSs were in coding sequence. PCR amplifications were conducted in 20 µl reactions. PCR product was digested with enzyme (AseI, DraI, HpaII, MseI, MspI, PstI, Taq. I) and were resolved on 1.5 % agarose gels to detect the polymorphism. Genetic relationship among 5 different origin groups was evaluated using Neighbor-joining tree and population structure. Principle Coordinate Analysis and Analysis of Molecular Variance were performed based on genetic distance matrix. As a result, the high genetic dissimilarities were observed between Korea and other 4 countries; genotypes between other countries had F ST values range 0.15 to 0.25 mean moderate differentiation; genotypes Brazil and Argentina had the lowest genetic dissimilarity. This means that Korean peanut varieties have fewer common origins with others, while those from the other four regions have more closely related origins. *Corresponding Author: Tel , thjun76@pusan.ac.kr PD-41 Hee Rang Yun, Jee Hye Kim, Hae Eun Kim and Jae Bok Heo * Perilla (Perilla frutescens) is an annual plant and grown in Asia. As one of important oil crops, its seeds are used to make a product and leaves are used as a vegetable, nevertheless, much is unknown about research of perilla. we already found a gene in RNA-seq results which may play a role in flowering time, and it was identified to be an ortholog of OsHd3a in rice. Hd3a promote heading under short-day conditions. Here, we focused on function of pfhd3a. Since tissue culture system using agrobacterium was developed, we made a pfhd3a-overexpressing plant in perilla using agrobacterium-mediated transformation. Also, we constructed knock-out mutant using CRISPR/Cas9 genome editing system. A reproducible shoot was induced from hypocotyl explants on MS basal medium supplemented with 3.0 mg/l 6-Benzylaminopurine (BA), 0.01mg/l indole-3-acetic acid (IAA), 250mg/l cefotaxime, 500mg/l carbenicillin and 1.2 mg/l PPT and candidates were selected. Rooting was induced on half-strength hormone-free MS medium. The transformants were confirmed by PCR of PPT resistance region. Next generation seeds were used for flowering phenotype check compared wild type under daylength condition. *Corresponding Author: Tel , jbheo72@gmail.com 151

208 PD-42 Jinwon Lee *1, Gynheung An 2, Soon Ki Park The plastid ribosomal proteins (PRPs) are essential for plastid protein biosynthesis, ribosome biogenesis and chloroplast development. To identify the leaf senescence mutants in rice, we screened T-DNA insertional mutant lines and isolated three different albino, seedling death phenotype mutants. Two mutants, PRP1 and PRP2 phenotypes were co-segregated with T-DNA.by genotyping analysis. PRP1 and PRP2 ribosomal proteins encode the large subunit components of chloroplast 50S ribosomal proteins, which contain chloroplast localization signal peptides, respectively. GUS assay showed that expression of PRP1-GUS detected in the root, sheath and leaf blade in seedling stage. *Corresponding Author: Tel , psk@knu.ac.kr PD-43 Jinwon Lee *1, Gynheung An 2, Soon Ki Park The plastid ribosomal proteins (PRPs) are essential for plastid protein biosynthesis, ribosome biogenesis and chloroplast development. To identify the leaf senescence mutants in rice, we screened T-DNA insertional mutant lines and isolated three different albino, seedling death phenotype mutants. Two mutants, PRP1 and PRP2 phenotypes were co-segregated with T-DNA.by genotyping analysis. PRP1 and PRP2 ribosomal proteins encode the large subunit components of chloroplast 50S ribosomal proteins, which contain chloroplast localization signal peptides, respectively. GUS assay showed that expression of PRP1-GUS detected in the root, sheath and leaf blade in seedling stage. *Corresponding Author: Tel , psk@knu.ac.kr 152

209 PD-44 Rahul Vasudeo Ramekar 1, Youn Sung Kim 2, Kyong Cheul Park 1* and Ju Kyong Lee 3* Maize (Zea mays ssp. mays) is among the most predominant food sources in the grass family (Poaceae), along with wheat (Triticum aestivum) and rice (Oryza sativa). Also, maize is an economically important crop and serves as a primary component of biofuels and animal fodder, as well as a source of byproducts used in textile, adhesive, and papermaking industries. Based on both endosperm and kernel constitution, maize can be classified into two categories, non-glutinous (common maize) and glutinous (waxy maize). In Korea, demand for both the types of maize is high and will increase exponentially in coming years. The maize genome is primarily composed of transposable elements, for which large and stable insertions generate variations that reflect selection during evolution. Mutator (Mu) transposon superfamily, a class of DNA transposons, is the most complex and active element in maize genome suggesting a unique role in plant evolution. In the present study, we have designed, a novel set of Mu-specific primers based on terminal invert repeats (TIR) and utilized a transposon insertion display method (MU-TD) for genotyping. Based on this method we have analyzed the distribution pattern of Mu insertion in teosinte (wild relative), sorghum (distant relative) and few domesticated maize accessions (dent, sweet and waxy). While 17% of the Mu insertion appeared to be fossil insertion shared between teosinte and maize, there is substantial evidence of recent activity of Mu element in investigated species. Apart from it, our other objective was to utilize the newly developed system for linkage analysis, identifying QTLs for yield-related traits, association mapping and to estimate genetic diversity in waxy and common maize. Results demonstrate the efficiency of the new system (Mu-TD) which will be useful for future maize breeding. *Corresponding Author: Tel , kyongcheul.park@kangwon.ac.kr Tel , jukyonglee@kangwon.ac.kr PD-45 Hye-Kyung Son, Kishor Doddanakatte Shivaramegowda, Somyung Lee, You Seok Kang, Hee-Jong Koh * In this study, we identified a stunted growth mutant from a japonica rice cultivar, Samgwang, treated with N-metyl-N-nitrosourea(MNU). The mutant showed dwarf, narrow leaf and sterile panicle. The retardation growth of the mutant initiated at the seedling stage and became severe at the reproductive stage. The plant height was reduced by 34% compared to that in wild type and showed significantly decreased first five internode length. The width of flag leaf was reduced by 46% compared to that in wild type. Anatomical analysis of the leaf suggested that less number of the vascular bundles and epidermal cells caused narrow leaf. Also, mutant plants showed low pollen viability and complete sterility. Genetic analysis indicated that the mutation was controlled by a single recessive gene. The F2 generations of a cross between mutant and Milyang23 were used for mapping. Candidate region was detected to a short arm of chromosome 5 near the marker S05000 and S05032 via BSA method. The mutant was fine-mapped at an interval of 84kb flanked by the markers NC and NdC Sequencing of the region identified that the mutant carries 11bp insertion in the third exon of LOC_Os05g03550, a gene which contains two SANT domains related to histone deacetylation. The insertion led to premature stop codon in cdna. Accordingly, a novel gene that cause inhibition of plant growth was isolated and the results may provide a basis for functional studies of the gene associated with growth and development of rice. *Corresponding Author: Tel , heejkoh@snu.ac.kr 153

210 PD-46 Rahul Vasudeo Ramekar 1, Yong Bog Kim 2, Byong Gon Choi 2, Sun Gang Choi 1, Ik Young Choi 1* and Kyong Cheul Park 1* 1 2 Ligularia fischeri is a popular edible herb in Korea containing broad ranges of pharmacologically important compounds. It is used in traditional medicine for treating infectious and inflammatory diseases. Despite its importance as herbal medicine, there are no transcriptome/genome sequences available in the public database limiting its research at molecular level. To address this issue, L. fischeri leaf transcriptome was sequenced using Pacific Biosciences single molecule long-read isoform sequencing platform. We identified 60,646 polished, high-quality non-redundant full-length transcripts with a total length of 116.5Mb. Among these 27,453 transcripts were annotated to known genes in different species based on non-redundant and uniport database. Functional classification using Gene ontology identified 11,279 transcripts of which majority were associated with the cellular and metabolic process. The Kyoto Encyclopedia of Genes & Genomes pathway analysis identified 1332 transcripts encoding 160 enzymes related to secondary metabolism with a higher number of transcripts for biosynthesis of antibiotics. Furthermore, we observed alternate splicing, in a total of 1,030 transcripts covering a total of 2250 isoforms with a variable of 2-11 isoforms. This data led us to identify 117 transcripts containing 271 isoforms involved in various metabolic pathways along with 36 transcripts containing 84 isoforms involved in various stress responses. This is the first detailed transcriptome analysis of L. fischeri. The resulting transcriptome along with the identified alternative splicing events provides insights into the biological process including the genes related to biosynthesis of characteristic secondary metabolites. This data will be valuable resource as transcriptome reference for further studies in the genetics and breeding of L. fischeri. *Corresponding Author: Tel , kyongcheul.park@kangwon.ac.kr Tel , choii@kangwon.ac.kr PD-47 Song-Yee Lee 1*, Deok-Ryeol Lee 1, Seung-Hyun Cho 1, So-Ra Choi 1, Eun-Ju Song 1, Young-Eun Song 1, Hyun-Ah Han 1, So-Hee Shin 1 1 Aromatic rice is a variety of rice with nice aroma and flavor. These rice were much higher price than high quality non-aromatic rice in international market. This study was performed to investigate the differences among the 31 varieties of aromatic rice and characterization of aromatic rice breeding line by using a GC-based electronic nose. We was conducted to optimize the analysis condition quantify the 2-acetyl-1- pyrroline(2ap) in the brown aromatic rice. The results of e-nose analysis showed that 2AP contents of Aromi and Jeonbuk10 were higher than those of domestic resources. As a result of SIMCA analysis of Jeonbuk10, We confirmed Daohuaxiang2 which was used as a crossbreeding combination of Jeonbuk10 showed the most similar aroma to Jeonbuk10. So this study would be useful to the fragrance analysis system for the aromatic rice breeding as objective data in the future. *Corresponding Author: Tel , song0922@korea.kr 154

211 PD-48 Su-Jin Choi 1, You-Ran Jang 1, Susan B. Altenbach 2, Min-Ki Lee 1, Sun-Hyung Lim 1, Jong-Yeol Lee 1* 1 2 It is well known that the composition of low-molecular weight glutenin subunits (LMW-GS) in wheat flour is important for end-use quality. However, the contributions of specific LMW-GS to quality have been difficult to assess because of the complexity of LMW-GS within a cultivar as well as the allelic variation between cultivars. Thus, the accurate and reliable determination of LMW-GS alleles in wheat germplasm is very important for breeding efforts. To locate individual LMW-GS corresponding to different alleles encoded by the Glu-A3, Glu-B3 and Glu-D3 loci, we analyzed a set of 15 near isogenic lines (NILs) from Aroona containing unique LMW-GS alleles in the same genetic background. Proteins in glutenin fractions were separated by two-dimensional gel electrophoresis (2-DGE) and the resulting protein patterns were compared to the pattern from Aroona. For most lines, the identifications of protein spots corresponding to LMW-GS alleles were consistent with results using a set of standard wheat cultivars for Glu-3. However, some spots in lines containing the Glu-B3b, Glu-B3g and Glu-D3c alleles differed from the previous study. To confirm their identities, these spots were excised from 2-D gels, digested with chymotrypsin and subjected to tandem mass spectrometry (MS/MS). We also developed a practical and optimized method for RP-HPLC analysis of LMW-GS using a Waters Xbridge BEH, C4 peptide column that results in better resolution than previous studies. The results will be used to identify LMW-GS alleles in germplasm prior to breeding and to screen for desirable LMW-GS alleles in wheat quality improvement. *Corresponding Author: Tel , jy0820@korea.kr PD-49 Jin Sun Kim 1, Su-Jin Choi 1, You-Ran Jang 1, Sun-Hyung Lim 1, Jong-Yeol Lee 1* 1 By establishing a system that can efficiently induce transgenic plants using plant tissue culture and molecular biology techniques, it can be applied to wheat to produce crops with useful traits. But wheat is considered a plant that is recalcitrant to genetically modify because of its low transgenic efficiency and genotype dependency. Therefore, various wheat transformation methods are underway to increase efficiency. So far, studies of transformation methods based on the characteristics of Korean wheat cultivars have been reported mainly on the regeneration efficiency and the confirmation of transient expression in embryogenic callus, and studies of systematic transformation method have rarely been progressed. Therefore, the aim of this study was to establish transgenic plants in Korean wheat cultivar Keumkang which try to introduce the centrifuges and embryo axis excision as well as the conventional methods to increase the transformation efficiency by biolistic method. Immature embryos (IEs) between the 13th and 23th days of DPA were dissected and used for transformation. A total of 9,030 IEs were tested with gold particle bombardment to introduce the GFP gene. The phosphinothricin (ppt) resistance test, the incidence of callus and the rate of green spot occurrence in Keumkang wheat were checked according to whether embryo axis was removed or not. Based on results of this study, it will provide valuable information for further development and commercialization of transgenic wheat. *Corresponding Author: Tel , jy0820@korea.kr 155

212 PD-50 김경민 1*, 김경훈 1, 신동진 1, 차진경 1, 김영진 1, 고종민 1 1 귀리 (Avena sativa L.) 는세계적으로벼과작물중에서밀, 옥수수, 벼, 보리다음으로생산량이많은작물로대부분이가축사료로쓰이나, 종실은단백질과지질이풍부하고체내이용률이높은식이섬유인베타글루칸이 3~6% 들어있어식용으로도그가치를인정받고있다. 귀리는탈곡후종실의껍질유무로구분되는데겉귀리는종실에내영과외영이라는껍질이있고쌀귀리는껍질이없다. 이런이유로겉귀리는수확후껍질을탈피하기위한노력이필요하지만쌀귀리는탈곡과함께껍질이제거되어식용으로이용하기유리하여우리나라에서는쌀귀리를식용으로이용하고있다. 하지만종실의껍질을제거한후에는겉귀리와쌀귀리가외관적으로비교가잘되지않고도입되는귀리가국내산쌀귀리와판별이어려운실정이다. 이에겉귀리와쌀귀리의탈부특성과품질이연관성이있는지알아보기위해본연구를수행하였다. 시험재료는국내에서재배되고있는겉귀리 7 품종과쌀귀리 5 품종총 12 품종을이용하였으며, 겉귀리와쌀귀리의탈부여부는수확후탈곡기를이용해탈곡되어나오는종실의껍질의유무로탈부성을확인하였다. 조단백질함량은질소 / 단백질분석기 (Kjeltec 8400) 를이용하였고, 조지방은조지방자동추출기 (SoxtecTM 2050) 를이용하여정량하였다. 베카글루칸은 Megazyme kit 를이용하여분석하였고, 지방산조성은 Gas Chromatography 를이용해분석하였다. 그결과단백질은쌀귀리가 13.45~16.56% 함량분포를나타내었고겉귀리는 12.95~15.31% 함량분포를나타내었으며, 지방은쌀귀리가 7.26~10.34% 의함량분포를나타내었고겉귀리는 7.53~10.82% 함량분포를나타내었다. 식이섬유인베타글루칸은쌀귀리가 4.19~5.05% 의함량분포르나타내었고겉귀리는 3.49~5.60% 의함량분포를나타내어귀리품종간에는품질특성이차이가나타났으나쌀귀리와겉귀리를구분할수있을정도는아니었다. 지방산조성비율은쌀귀리가겉귀리에비해올레인산 (C18:1, Oleic acid) 과스테아르산 (C18:0, Stearic acid) 이높은비율을나타냈으며, 겉귀리는쌀귀리에비해리놀레산 (C18:2, Linoleic acid) 과리놀렌산 (C18:3, Linolenic acid) 이더높은비율을보였다. * 교신저자 : Tel , raiders87@korea.kr PD-51 Beom-Kyu Kang *, Jeong-Hyun Seo, Eun-Young Oh, Hyun-Tae Kim, Hong-Sik Kim, Sang-Ouk Shin, Chang-Hwan Park, Do-Yeon Kwak Sprouting speed of soybean [Glycine max Merr (L.)] seed is various among genotypes. It has been known that the contents of lipid and sugars were changed in the germination process and several enzymes relating to glyoxylate cycle were also activated during germination of oil seeds. These factors seemed to be associated with energy distribution mechanism in soybean seed. To determine important factors for sprouting speed of soybean seed, four genotypes with different chemical composition and sprout characteristics, Pungsannamul, Wonheug, S04 (low saturated fatty acid, SFA) and S11 (high SFA), were grown for soybean-sprout in dark chamber for five days with 20 air condition and irrigating water 3 minutes per every 4 hours. Sprout characteristics, amounts of chemical components (lipid, fatty acid and sugar) and the level of gene expression (ICL : isocitrate lyase enzyme gene) of cotyledon were investigated during the sprouting process. Sprout length of Wonheug was 20.1cm and much higher than other genotypes. Lipid and sugar contents in seed were different among soybean genotypes, but significant variation of their contents during sprouting process was not observed. The fatty acid composition was also not changed during sprouting process. The level of ICL gene expression of Wonheug was highest with a value of 15.76(p<0.01) in 1-day old sprout as compared to that of Pungsannamul(2.66), S04(1.82), and S11(1.10). These results showed that the contents of lipid and sugar and the composition of fatty acid were not major factors to determine sprouting speed of soybean. The level of ICL gene expression might be related to the sprouting speed in soybean. *Corresponding Author: Tel , hellobk01@korea.kr 156

213 PD-52 So-Jin Lee 1*, Yong-Hee Kwon 1, Chun-Hwan Kim 1, Chan-Kyu Lim 1, Seung-Yeob Song 1, Doo-Gyung Moon 1 1 Many functionality roles are widely known about tea leaves such as amino acids like theanin and polyphenol like catechin can help psychological stability or prevent various illness. These components are contained different volume depending on tea cultivars and cultivation style. For breeding new cultivars which are contained many or unique functionality components, we tried to distinguish domestic and foreign tea cultivars using extracted components from fresh tea leaves. FT-IR analysis were performed about 26 tea cultivars including 10 domestic tea, 12 Japanese tea, 3 Taiwan tea and 1 Chinese tea. According to spectra result, significant spectra were identified on three spectrum range and some cultivars had remarkable spectra. Part of cultivars like Yabukida, Meiryoku, Sangnok were assumed certainly different from others and many of cultivars which were nearly located from each other were identified following results of Principal Component Analysis(PCA), Partial Least Squares Discriminant Analysis(PLSDA) and Hierarchical Cluster Analysis(HCA) dendrogram. These results mean tea cultivar which have similar characteristics are appeared similar components. Thus, breeding line and characteristic were important for composing various component and this results will provided basic information for breeding new functionality tea cultivars. We will perform metabolome analysis about target component by adding actual measurement data of polyphenol contents in further study. *Corresponding Author: Tel , dlthwls324@korea.kr PD-53 김영천, 차아라, 이상협 * 배추과작물에서주로발견되는 glucosinolates 유래의분해산물은인체내에서항암작용을하거나, 돌연변이를억제하는등다양한생리활성효과를나타낸다고알려져있다. 현재까지 120 여종의 glucosinolate 가다양한배추과작물에서밝혀졌다. 특히 glucosinolate 는식물체가상처를받을경우 myrosinase 라는효소작용으로 isothiocyanate, glucose, 산성황산염등으로분해된다고알려져있다. 이중에서 isothiocyanate 는강한항암, 항균, 살충작용과같은생체방어반응을유도하는것으로알려져있다. 브로콜리 (Brassica oleracea) 는배추과작물로항암, 고혈압예방, 심혈관질환예방을비롯한다양한효능이있다고알려지면서타임지가선정한세계 10 대푸드중하나에속하기도한다. 따라서국내에서도 2000 년도이후소비량이급속히증가되고있는실정이다. 브로콜리의대표적인기능성물질은 sulforaphane (S-methylsulfinylbutyl isothiocyanate) 으로알려져있다. 브로콜리는 sulforaphane 의전구물질인 glucoraphanin 을합성해서축적하고있다가, 조직이기계적인상처를입을경우 myrosinase 에의해분해되면서 sulforaphane 과 sulforaphane nitrile 을함께생성된다고알려져있다. 따라서브로콜리가가지는다양한생리활성효능은 glucoraphanin 함량과비례한다고여겨지며, 함량을높이는시도가진행되어왔다. 최근에는기존품종대비 glucoraphanin 함량이 2-3 배증가된품종이개발되어시장에출시되는상황이다. 영국연구진에의해서 Glucoraphanin 함량은높은야생 brassica (Brassica villosa) 자원이수집되었고, 이후 15 년간의반복적인교배를통해서 2011 년 Beneforte 라는품종이몬산토에서출시되었다. 하지만전통적인교배육종을통해서 glucoraphanin 고함량품종을육성하기에는너무나많은제약요건이많다. 따라서본연구진은 CRISP/Cas9 이라는최신유전자편집기술을육종계통에직접적용하여단기간에 Glucorapanin 함량이증대된브로콜리품종을육성하고자한다. Acknowledgement: This work was supported by Bio-industry Technology Development Program of ipet (Korea Institute of Planning and Evaluation for Technology in Food Agri-culture and Forestry) and Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center No. PJ ) of Rural Development Administration of Korea. * 교신저자 : Tel , sanglee@sejong.ac.kr 157

214 PD-54 Da-Hye Kim 1, Bora Park 1, SangKyu Park 1, Jong-Yeol Lee 1, Sun-Hyung Lim 1* 1 Anthocyanins, pigments that accumulate in flowers and fruits, have attracted a lot of attention due to their antioxidant properties. Anthocyanin biosynthesis is transcriptionally regulated by the MBW complex composed of R2R3-MYB, bhlh, and WD40. To identify the regulatory mechanism in anthocyanin production in rice, here, the molecular and functional properties of three rice homologues corresponding to the three components of the MBW, OsC1 (R2R3-MYB), OsB2 (bhlh), and OsTTG1 (WD40) were investigated. We analyzed the subcellular localizations of these proteins, showing that OsC1 and OsB2 localized in nucleus, and OsTTG1 localized in cytoplasm as well as nucleus. Yeast two-hybrid showed the interaction between each component of the MBW. In addition, the gene expression with steroid receptor-based inducible activation system revealed that the transcript levels of anthocyanin biosynthetic genes were increased by co-expression of the three genes. These results suggest that anthocyanin biosynthesis in rice could be directly activated by MBW complex composed of OsC1, OsB2, and OsTTG1. Complementation analysis of tt2, tt8 and ttg1 Arabidopsis mutants showed that anthocyanin and proanthocyanidin accumulations in their seeds were recovered by OsC1, OsB2, and OsTTG1 expression, respectively, and trichome development in the leaf of ttg1 was restored by OsTTG1 expression. These indicate that OsC1, OsB2, and OsTTG1 can be a functional combination as an MBW complex in planta, and the MBW regulatory machineries are well conserved in dicot and monocot plants. *Corresponding Author: Tel , limsh2@korea.kr PD-55 Hyo-Ju Lee, Ye Rim Lee, Yeo Jin Jung, Jong Hee Kim, Dong Hyun Kim, Jae Uk Lee, Hee Kyong Kim, Chang Sun Sin, Ki Hong Nam, Jeong Sun Park, Yu Jin Jung, Kwon Kyoo Kang * Starch is a major component of human diets. The physio-chemical properties of starch influence the nutritional value of starch and the functional properties of starch containing foods. A population of 233 breeding lines of rice was analysed for variation in 17 rice starch synthesis genes, encoding seven classes of enzymes, including ADP-glucose pyrophosphorylase (AGPases), granule starch synthases (GBSS), soluble starch synthase (SS), starch branching enzyme (BE), starch debranching enzyme (DBE) and starch phosphorylase (SPHOL) and phosphate translocator (GPT1). To obtain variant plants with diverse starch structure in endosperm, we used the CRISPR / cas9 system for the starch biosynthesis-related 22 genes, respectively. Sixty-nine Cas9 vectors constructed by selecting three sgrnas for each of the 22 genes were introduced by the Agrobacterium method and obtained 3960 transgenic T0 plants. As a result of the NGS analysis of the transgenic plants, 2900 plants were selected with 75% of the mutated plants at the target site. In the compilation of genes, base addition and deletion were observed at the target site. Homo, Hetero and Bi genes were also found. Homo plants breed T1 seeds and 60 Tl seeds were seeded to discriminate resistance and susceptibility with 4 ppm Barstar treatment. Also, the presence of T-DNA was reaffirmed by a bar-strip kit from susceptible individuals. Among the susceptible plants, individuals with very normal phenotype were selected and recognized as Null line. The amylose and amylopectin contents in the Null lines were varied and it could be used as a rice breeding material with double mutant breeding and new type of starch constitution. *Corresponding Author: Tel , kykang@hknu.ac.kr 158

215 PD-56 Namhee Jeong 1, Jaebuhm Chun 1, Dool-Yi Kim 1, Mina Jin 1, Jung-Kyung Moon 2, Man Soo Choi 1* 1 2 Developing a core collection in soybean, one of the most important crop resources worldwide, is an important and valuable task. Here, we developed a Korean soybean (Glycine max) core collection consisting of 430 accessions, using Affymetrix Axiom R 180k (180,961) SoyaSNP genotyping array and GenoCore method. In addition, we performed genetic diversity, morphological trait and population structure analyses to construct the core collection from a total of 2,872 collections. Furthermore, to evaluate the representative nature of the developed core collection for entire germplasm accessions, genome-wide association studies (GWAS) for various important, agronomic traits were conducted and compared. Sample call rates less than 97% were excluded, along with duplicate samples with more than 99.9% similarity, according to genotype analysis using Axiom R 180K SoyaSNP from the entire collections. As a result, we constructed the core collection of genetic diversity that reflects 99% of the total collections, including 430 soybeans. Finally, we developed the Korean soybean core collection, approximately 15% of the total collection. The Korean soybean core collection developed in this study was divided into 6 groups based on a population structure analysis. Further, morphological aspects of the Korean soybean core collection were confirmed to represent an average of 18.1% of the total collection. In addition, we validated the core collection through GWAS for already known controlling genes such as days to flowering, flower color, pubescence color, and growth habit. Consequentially, the Korean soybean core collection developed in this study should provide useful material for both soybean breeding programs and GWAS. Acknowledgement: This work was supported by a grant from the Next-Generation BioGreen 21 Program (No. PJ ), Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , mschoi73@korea.kr PD-57 Dong Jin Lee 1, Chang-Kug Kim 2, Yi Lee 3, Chang Pyo Hong Schisandra chinensis (Omija) is a fruit-bearing vine, and its purple-red berries are described as having five tastes. In particular, the seeds contain lignans, having beneficial effects on health. To obtain transcriptomic data that offers a more comprehensive view of fruit development in S. chinensis, we generated genome-wide transcriptome data from different tissues using PacBio isoform sequencing (Iso-Seq) technology. A total of 132,856 assembled transcripts were generated with an average length of 1.9 kb and high assembly completeness. Of those unigenes, 71.6% were predicted to be complete full-length (FL) ORFs and exhibited a high gene annotation rate. Furthermore, we successfully identified unique full-length genes involved in polyphenol synthesis. Based on these unigenes, we have identified the expression change of genes from different ripening stages of fruit, thus extrapolating regulatory networks genes, especially regulators, related to polyphenol synthesis. In conclusion, our results suggest that long-read, full-length or partial-unigene data with high-quality assemblies are invaluable resources as transcriptomic references in S. chinensis and can be used for comparative analyses for fruit development in closely related medicinal plants. *Corresponding Author: Tel , changpyo.hong@theragenetex.com 159

216 PD-58 Youn Jung Choi *, Young Ran Lee, Yun Im Kang Freesia (Freesia hybrida Hort.) Blue Angel was developed for the cut flower in the National Institute of Horticultural Herbal Science in This hybrid was crossed and selected from a seedling of 04C3-43 and CV06C3-009 with single blue and pink petals, respectively, in Morphological characteristics of the selected freesia hybrid were investigated for 5 years from 2011 to 2016, and then it was named Blue Angel in Blue Angel has blue single petals (RHS, VG84A). The average flower width is 5.8 cm and the average yield is 6.0. The growth of the plant shows vigorous and the average height is 114.7cm. The average number of floret per stalk was 11.7, and 9.8 cm length that of control cultivar Cascade, 10.3 and 10.2 cm length, respectively. The average days to first flowering of Blue Angel, days and the average vase life and yield is 12.3 days and 6.0 cormlets per plant, respectively. *Corresponding Author: Tel , lillium@korea.kr PD-59 Youn Jung Choi *, Young Ran Lee, Yun Im Kang Freesia (Freesia hybrida Hort.) Pink Bubble was developed for the cut flower in the National Institute of Horticultural Herbal Science in This hybrid was crossed and selected from a seedling of Kristie and the seedling of Michelle and Rpssini crossed with White Lace in Morphological characteristics of the selected freesia hybrid were investigated for 5 years from 2011 to 2016, and then it was named Pink Bubble in Pink Bubble has pink double petals (RHS, RPN57A). The average flower width is 5.8 cm and the average yield is 5.0. The growth of the plant shows vigorous and the average height is 104cm, and it is higher than about 16 cm that of control cultivar Honeymoon. The average number of floret per stalk was 10, and stalk was 9.0 cm length that of control cultivar Honeymoon, 10 and 10.3 cm length, respectively. The average days to first flowering of Pink Bubble, 118 days and the average vase life and yield is 11.7 days and 5.0 per plant, respectively. *Corresponding Author: Tel , lillium@korea.kr 160

217 PD-60 Seon-Hwa Bae 1, Kyeong-Seong Cheon 1, Ye-Ji Lee 1, Hong-Il Ahn 1, Myoung-Hee Lee 2, Jeong-Hee Lee 3, Ung-Han Yoon 1, Jundae Lee 4, Tae-Ho Kim 1* Perilla species have been known to belong to the Lamiaceae family of plants and are widely grown in East Asia. They also have been taken as a traditional herbal medicine or a functional food for a long time. To find single nucleotide polymorphisms (SNPs) in Perilla species and analyze a phylogenomic relationship, we determined the complete sequences of the chloroplast genome (cpdna) and 45S nuclear ribosomal DNA (45S nrdna) of six cultivated and three wild Perilla species. The complete cp genome ranged in size from 152,588 bp to 152,656 bp and the length variation in cp genomes was 68 bp. The length of the 45S nrdna ranged from 6,235 bp to 8,303 bp and the main variations of length differences was caused by the intergenic spacer (IGS) region. Comparative analysis of the cp genome sequences of nine Perilla species revealed low genetic diversity at the intra- and inter-species level. Using SNP analysis of cpdna, we detected 42 synonymous SNPs (sysnps) from 27 genes and 37 non-synonymous SNPs (nssnps) from 15 genes. A comparison of the 45S nrdna sequences revealed two SNPs in the 18S rrna, five SNPs in the 26S rrna, three SNPs and two InDels in the internal transcribed spacer (ITS) 1 region, and six SNPs in the ITS 2 region. Our phylogenomic analysis suggests that the tetraploidization of Perilla cultivars may have arisen from the P. citriodora genome. The genotyping data from this study may be used to develop molecular markers associated with useful traits for use in Perilla breeding. Acknowledgement: This research was carried out with the supports by National Agricultural Genome Program (PJ013355) of National Institute of Agricultural Science, Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , thkim@rda.go.kr PD-61 Sin-Gi Park 1*, Kesavan Markkandan 1, In-Gang Shin 1, Boram Choi 1, Chang Pyo Hong 1, Jung Sun Kim As an increasing number of plant genome sequences become available, it is clear that gene content varies between individuals, and the challenge arises to predict the gene content of a species. However, the high quality assembled genome is important for understanding the genome structure, genetic variation and evolution on crop species. Here, we present the comparison of different sequencing platform and de novo assembly protocol for Brassica rapa cultivar chiffu genome. For this, we generated two types sequencing data. 1) short-reads paired-end data from Illumina HiSeq4000 platform (385.23X). 2) Long-reads Single Molecule Real Time (SMRT) data from PacBio Sequal platform (61.64X). Furthermore, we assembled B. rapa genome using three different tools, in order to achieve high quality assembly (Platanus [Illumina short-read] - 391Mb, CANU [PacBio long-read] - 353Mb, FALCON-Unzip [PacBio long-read] - 320Mb). We observed that, the assembled genome size was less than the estimated genome size (452Mb) of B. rapa based on k-mer measurement. In addition, assembly results revealed that the contig N50 size was longer in long-read assembly than short-read assembly. To address the assessment and completeness of assembled genomes, we used BUSCO and CEGMA tools revealed complete matches of single copy orthologs in B. rapa. Finally, we confirmed the assembled B. rapa genome with the previously reported B. rapa (Chiifu ) genome using QUAST for assessment. Besides, our results are the first step towards efficient comparison of assembly protocol and highlight the potential sources of error in future production of a B. rapa pangenome. *Corresponding Author: Tel , singi.park@theragenetex.com 161

218 PD-62 김경훈 *, 차진경, 신동진, 김경민, 김영진, 현종내, 고종민 유색밀은종피에자색또는흑색이착색되어색을띄는밀이다. 유색밀아리흑을실험재료로사용하여색소성분과영양성분을분석해일반밀금강밀과비교하였다. HPLC 를이용해안토시아닌색소계열별함유량을비교했을때아리흑에서는 C3G(cyanidin-3-O-glucoside) 가 1.53 μg /g, Pn3G(peonidin-3-O-glucoside) 는 0.38 μg /g 검출되었고, 금강밀에서는검출되지않았다. 아리흑의 DPPH 라디칼소거활성을측정했을때, 40% MeOH 에서 30 분에 5.32±2.22%, 60 분에 19.45±2.82% 였고, 80% MeOH 에서는 30 분에 12.18±0.83%, 60 분에 22.06±3.18% 로금강밀보다높게나타났다. 통밀가루로분쇄한후알칼리가수분해방법을이용해결합형탄닌과총페놀성화합물, 항산화능등을분석한결과, 유색밀의결합형탄닌함량은 ±83.27( μg catechin Eq./g), 총페놀성화합물함량은 ±18.37( μg GAE/g) 로모두금강밀의탄닌함량 ± 20.62( μg catechin Eq./g), 총페놀성화합물함량 ±17.21( μg GAE/g) 보다높게나타났다. Trolox equivalent antiocidant capacity(teac) 항산화능측정결과유색밀은 ±28.64( μg trolox Eq./g) 로금강의 ±4.77( μg trolox Eq./g) 보다높았다. 아리흑과금강밀통밀가루의무기이온함량비교결과, 칼슘성분은아리흑에서 35.65mg/100g 로금강밀보다 5.95mg/100g 높았고철은 4.76mg/100g 로금강밀보다 mg/100g 높았으며, 인은 284.5mg/100g 로금강밀보다 mg/100g 높았다. 그러므로유색밀을통밀로활용하여가공품을만들경우기존의일반밀보다기능성성분및영양성분이풍부할것으로생각된다. * 교신저자 : Tel , k2h0331@korea.kr PD-63 이진석 1*, 손범영 1, 김정태 1, 배환희 1, 백성범 1, 정태욱 2, 김병주 황금맛찰은자식계통 KY37 을종자친 ( 모본 ) 으로하고 KY29 을화분친 ( 부본 ) 으로하는단교잡종찰옥수수이다. 황금맛찰은 2012 년과 2014 년수원에서생산력검정시험을수행하였고 2015 년 ~ 2017 년전국 5 개지역에서지역적응시험을수행하였으며 2017 년농촌진흥청직무육성신품종선정위원회에서그우수성이인정되어직무육성신품종으로선정되었다. 황금맛찰은카로티노이드를함유하고있어이삭의색이황색이고줄기에굴곡이있으며수염에안토시아닌색소가강하게발현된다. 황금맛찰은출사일수가 69 일로표준품종인일미찰보다 2 일빨랐고착수고는낮았으며분지수는적었다. 이삭길이는일미찰과유사하였고이삭폭과착립장률은다소작았다. 황금맛찰은깨씨무늬병, 그을음무늬병, 조명나방등병해충저항성과내도복성에서일미찰과차이가없었으며황금맛찰의 10a 당이삭수와이삭중은각각 6,389 개 /10a, 1,246kg/10a 으로일미찰과유사하였다. 황금맛찰의베타카로틴함량은 16.4 μg /100g 으로일미찰보다 8 배이상높았고과피두께, 백립중, 전체기호도등에서는일미찰과유의한차이는없었다. 황금맛찰은찰옥수수열성유전자 (wx) 를보유하고있어일반옥수수, 초당옥수수 (sh, sh2, bt 등 ), 단옥수수 (su, se) 등다른종류의옥수수꽃가루로수정되면고유한특성이사라지므로반드시시간적혹은공간적으로격리재배하여야하고조명나방에감수성이어서적기방제가필요하며품질이우수한찰옥수수생산을위하여적기에수확 ( 출사후 22~23 일 ) 하여야한다. 본연구는농촌진흥청연구사업 ( 세부과제명 : 식용옥수수우량계통육성시험, 세부과제번호 : PJ ) 의지원에의해이루어진것이다. * 교신저자 : Tel , z @korea.kr 162

219 PD-64 하건수 1*, 송윤호 1, 조영래 1, 오혜진 1, 조윤상 1, 이지우 1, 김상수 1, 송원종 2, 강철홍 강원도농업기술원에서육성한비린내없는기능성소립검정콩 약선콩 을이용한고품질기능성두유생산을위하여일반적으로시중에유통되는쥐눈이콩과비교하여두유가공시관능성을평가하였다. 이는특수목적콩육성후현장실용화를위해서육성품종의기능성물질특성뿐아니라소비자가직접접하는제품형태의관능성평가결과를수반하여산업화하기위함이다. 관능성평가는기존제품대비약선콩두유의맛품질경쟁력을파악하기위하여서울및수도권거주 30~50 대여성들을대상으로관능평가전문기관인 센소메트릭스에서수행하였다. 평가방법은블라인드검사로객관적결과도출후시장에서인식되는상품성수준파악을위하여제품컨셉을제공한후같은방식으로평가하였다. 제품컨셉은 이번에제공되는제품은예로부터건강에더좋다고알려진국산검은콩 ( 쥐눈이콩 ) 만껍질채갈아넣어약콩의풍부한영양성분을그대로담았습니다. 또한설탕이나유화제등을첨가하지않아더욱건강한제품입니다. 이다. 주요관능속성평가는맛에대한종합기호, 외관, 향미, 입안느낌, 뒷맛의세부기호를평가하였고, 인지강도와희망강도는두유맛, 고소한맛, 단맛, 비릿함, 목넘김부드러움, 텁텁함속성을평가하였다. 결과분석은 SensMine( 센소메트릭스 ) 를활용하였으며, 종합선호결과는각시료에대한선호선택빈도를선호유의차검정 (Binomial Test) 하여 95% 신뢰수준차이에서비교분석하였다. 기호및강도평가결과는 LSD(Least Significant Difference) 방법으로 95% 신뢰수준에서다중비교검정하였다. 관능품질개선방향에대해서는종합기호도와관능속성의인지및희망강도반응값에대해 Ideal Profile Method(IPM) 방법으로분석하였다. 종합평가결과약선콩제품은기존재래종제품에비하여종합선호, 기호는물론세부기호에서높게평가되었다. 세부속성강도에서약선콩은대표적인긍정속성중하나인목넘김부드러움의경우뚜렷하게강하고, 부정속성중의하나인비릿함은뚜렷하게약한것으로평가되어관능속성프로파일관점에서유리하였다. 이러한결론은약선콩두유제품은기능성측면과함께맛품질측면에서도높은경쟁력을가진것으로평가된다. * 교신저자 : Tel , redclover@korea.kr PD-65 Gyu Tae Park 1, Jagadeesh Sundaramoorthy 1, Hak Soo Seo 2, Jong Tae Song 1* 1 2 To investigate the molecular mechanism of development contributing to coiled morphology, screening was carried out from Arabidopsis activation tagging lines obtained by activation T-DNA treatment that have curly/wavy morphology. The mutant named cbr, was found to have a wavy and curly morphology with coiling branches. Plasmid rescue and genomic southern blot analysis revealed the site of T-DNA insertion in the genome. RT-PCR was performed to monitor expression levels of the genes adjacent to the T-DNA integration site and showed the activation of an E3 ubiquitin ligase gene. Database search revealed that the protein with the C3HC4 type RING domain belongs to a family of E3 ubiquitin ligases. Complementation test by overexpression and RNA interference of the gene showed that activation of the novel gene caused the cbr mutant phenotypes. Ubiquitylation has been linked virtually to every cellular process including plant development. E3 ubiquitin ligase has been reported to recognize target proteins that are to be ubiquinated for further degradation by the proteasome complex. Therefore, we are performing 2-DE and Y2H experiments to find specific substrate(s) of the novel E3 ubiquitin ligase gene. Acknowledgement: This work was carried out with the support of Cooperative Research Program for Agricultural Science & Technology Development (Project No. PJ ) by the Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , jtsong68@knu.ac.kr 163

220 PD-66 Chi-hwan Kim 1, Junki Lee 1, Eunkyoung Jun 1, Yoojin Kim 1, Hyang Sook Chun 2 and Gyoungju Nah 1* 1 2 DNA barcodes developed from organellar genomes in mitochondria or chloroplast have been playing important role in distinguishing authentic food materials from fraud ones. Our primary objective is to develop a comprehensive DNA barcode database, which will serve as a resource for future scientific authentication for food safety. In order to build such database, we used Tripal open-source databaseinfrastructure. The Tripal-based DNA barcode database consists of three interconnected modules: search, profile, and analysis. These modules make the database enable both single marker review and batch analysis with multiple kinds of data and multiple species. To date, we have deposited more than 200 barcode markers designed from mitochondrial and chloroplast genomes. Equipped with specialized functional modules and modernized visualization tools, and populated with multiple kinds of data, our database will provide a quick and easy data analysis platform for both industry and academia. This research was supported by a grant (17162MFDS065) from Ministry of Food and Drug Safety, Korea, in Keywords: Chloroplast genome, DNA barcode marker, Database, food authentication *Corresponding Author: Tel , gjnah@snu.ac.kr PD-67 남궁민 *, 류시환, 최재근, 박종열, 박기진, 최승출, 김문종, 용우식, 남경남, 윤석원, 최준근 베타카로틴 (β-carotene) 은동물의대사작용을통해비타민 A 로전환될수있는카로티노이드에속하는주요성분으로시각과관련된망막질환과백내장등의예방에매우중요한역할을한다. 카로티노이드는주로빨강색, 노란색, 주황색과일또는채소에많이함유되어있으며심장병및암에도의학적효과가있다고한다. 최근이러한식물의천연색소, 식이섬유, 단백질원료등을활용하기위한연구가진행되고있으며, 색소성분의함량이높은품종육성이육종가의주요목표가되고있다. 본연구에서는국제밀옥수수연구소 (CIMMYT) 에서개발한베타카로틴생성에관련된 hydroxylase 1(crtRB1) 유전자를선발하기위한두가지분자마커를 (5 TE, 3 TE) 활용하여 F 3 분리세대 (17Cr) 옥수수의계통을분석및선발하였다. 또한, 선발된계통에대한베타카로틴의함량을평가하고자고성능액체크로마토그래피 (HPLC) 분석을실시하였다. 본연구에서는 704 개의분리계통을평가하였고, 각각의 DNA 는잎에서추출하여 crtrb1-3 TE, 5 TE 두개의프라이머로분석하였다. 분석결과한개의특이증폭밴드를가진 39 계통을선발하였다. 선발된계통및그렇지않은계통들을베타카로틴함량분석을한결과 17Cr243 계통에서최고 38.6 μg /g 의베타카로틴이검출되었고, 이수치는선발되지않은계통들을분석한함량중에서불검출을제외한최저함량인 1.6 μg /g(17cr422) 에비해약 24 배높았다. 본연구의결과를통해 crtrb1 분자마커를활용한베타카로틴고함유옥수수계통선발은색깔을통한육안으로선발하는육종방법에비해시간과노력이절감되며정확한선발을할수있을것으로기대된다. 사사 : 본연구는 GSP 식량종자사업 (PJ ) 및기관고유사업 ( 배가반수체육종효율증진연구 ) 의지원에의해이루어진것임 * 교신저자 : Tel , ngm0623@korea.kr 164

221 PD-68 Tackmin Kwon 2 and Jaesung Nam 1* 1 2 Syringin, sinapyl alcohol 4-O-glucoside, is well known as a plant-derived bioactive monolignol glucoside. In Arabidopsis, recombinant chimeric protein UGT72E3/2 has been previously reported to lead to significantly higher syringin production than the parental enzymes UGT72E2 and UGT72E3. In this study, we demonstrated that the production of syringin in Arabidopsis leaves can be enhanced through metabolic regulation. To enhance metabolic flow through the phenylpropanoid pathway and maintain a high homeostatic concentration of sinapyl alcohol in the plant leaves without cosuppression, we combined various UGT72E-Myc with F5H encoding ferulate 5-hydroxylase or/and Myb58 encoding the lignin biosynthesis transcriptional activator in a single vector. The results showed that transgenic plants expressing simultaneously UGT72E3-Myc+F5H+Myb58 accumulated syringin 3 ~ 6 times more in their leaves compared to transgenic plants expressing UGT72E3-Myc+F5H or UGT72E3-Myc+Myb58. More importantly, transgenic plants expressing UGT72E3-Myc+F5H+Myb58 exhibited the highest ratio of syringin to coniferin, suggesting that is optimal combination of genes on regulating metabolic flow for syringin production in plants. We also are attempting to transform this multiple gene expression vector into several mutants blocking the metabolic branch pathways from phenylpropanoid pathway, such as chs mutant deficient chalcone synthase that catalyses the flavonoid/isoflavonoid biosynthesis pathway and aldh mutant deficient aldehyde dehydrogenase that bypasses sinapyl aldehyde to sinapate instead of sinayly alcohol. These metabolic regulation methods are of value in the development crop plants efficiently producing biologically active secondary metabolites. *Corresponding Author: Jaesung Nam, jnam@dau.ac.kr PD-69 Jagadeesh Sundaramoorthy 1, Gyu Tae Park 1, Seokhyung Lee 1, Jeong-Dong Lee 1, Hak Soo Seo 2 and Jong Tae Song 1* 1 2 Saponins are a group of secondary metabolites available in soybean [Glycine max (L.) Merr.]. Soybean saponins are classified as group A and DDMP saponins. Group A saponins are undesirable component of food products due to its bitterness and astringency. In contrast, DDMP saponins and their derivatives are not possessing bitterness and astringency but beneficial to human health when consumed as regular diet. The objective of the present study was to identify and characterize the gene which is encoding a protein responsible for biosynthesis of DDMP saponins, and then finally to reveal a role of saponins in soybean plants. We isolated two EMS mutant lines (PE2248 and PE2371) with DDMP-deficient saponins. The breeding cross has been made with these two mutants along with two cultivars, Pungsannamul and Uram to study the segregation and genetic linkage analysis, respectively. The segregation analysis showed that the mutant phenotype is controlled by a single recessive gene. The position of locus (Sg-9) involved in the biosynthesis of DDMP deficient saponins was mapped using bulk segregation analysis and fine mapped on chromosome 16 (130 kb) between two SNP markers. The genomic sequencing of Sg-9 gene showed a single nucleotide polymorphism in PE2248 (G626A) and PE2371 (C137T) mutant lines and new alleles were designated sg-9-a and sg-9-b, respectively. Allelism test between two mutants PE2248 and PE2371 exhibited that the mutation sites lie on the same gene. The enzymatic assay showed that mutant recombinant proteins showed lower glycosyltransferase activity than wild-type Sg-9. Further, tissue-specific expression study indicated that Sg-9 expression corresponds to the accumulation of DDMP saponins in soybean seeds. These results support that Sg-9 is likely to play a role in the biosynthesis of DDMP saponins in soybean. This work was carried out with the support of Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ ) Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , jtsong68@knu.ac.kr 165

222 PD-70 Min Jeong Hong 1, Dae Yeon Kim 2, Hong-Il Choi 1, Yeong Deuk Jo 1, Sang Hoon Kim 1, Soon-Jae Kwon 1, Joon-Woo Ahn 1, Yong Weon Seo 2, Jin-Beak Kim 1* 1 2 F-box protein is components of SCF ubiquitin ligase complex, serves as substrate adaptors to mediate the degradation of a large number of regulatory proteins involved in diverse processes. F-box protein plays important roles in the regulation of various development processes in plants. About 1796 F-box genes have been identified in the wheat genome, but their detailed functions remain unknown. We isolated five F-box protein genes from wheat grain development. The cdnas encoding TaKFB1, TaKFB2, TaKFB3, TaKFB4, and TaKFB5 contained 364-, 450-, 354-, 383-, and 457-bp open reading frames, respectively, and all deduced TaKFBs contained a F-box domain (IPR001810) and Kelch repeat type 1 domain (IPR006652) except TaKFB2. The TaKFBs showed elevated expression during grain development stages of color pigmentation. To clarify how TaKFBs and SKP proteins combine in wheat, we examined whether the 5 TaKFB proteins showed specificity for 6 SKP proteins using a yeast two-hybrid assay. And also, a yeast two-hybrid screen performed to search for proteins capable of binding the TaKFB proteins identified an interaction with partner of paired. To examine the subcellular localization of TaKFBs, we transiently expressed TaKFB-GFP fusions in tobacco leaves; the TaKFB-GFP fusions were detected in the nucleus and the cytoplasm. These results will provide useful information for further functional studies on wheat F-box proteins and their possible roles. *Corresponding Author: Tel , jbkim74@kaeri.re.kr PD-71 Yu Mi Choi *, Do Yoon Hyun, Sukyeung Lee, Myung-Chul Lee, Sejong Oh, Hocheol Ko, On-Sook Hur, Munsup Yoon, Gyu Taek Cho 1 This study was conducted to provide information by evaluating the nutritional and functional components as well as the agricultural characteristics related to the possibility of growing tropical and subtropical minor legume crops in Korea. The materials were used 260 accessions including lentil(68 acc.), lima bean(48acc.), chick pea(72acc.) and guar(72acc.). Four Legume crops were sown on 14 th March, and planted on 10 th April 2017, at green house in Nongsaeng myeongro, Jeonju city. Days to flowering were ranged from 48 to 85 days, those of lentil was the shortest with 48 days, those of lima bean was the longest with 85 days. The average days of growth were ranged from 82 days(lentil) to 164 days(guar). The maturity period was mostly distributed from the last of May to the middle of September. The flowering days of common bean controls, Gangnangkong# 1 and Shinseondu were 55 days and 54 days, days of growth of those were 102 days and 98 days, respectively. The average 100 seed weight by crops was 2.2g of lentil, 80.5g of lima bean, 22.9g of chick pea, 3.8g of guar. The average of crude protein contents in legume crops was ranged from 17.5% to 26.4%, the highest in guar, and the lowest in chick pea. The average of crude oil contents in legume crops was generally low from 0.8%(lentil, lima bean) to 4.3%(chick pea). The crude protein contents of common bean controls was between 17.2 % and 19.7 %, % of the crude oil, and 4.0 % of the crude fiber. The average of crude protein and crude fiber contents in legume crops were higher than common bean and the crude oil contents of those were similar with that. According to the characteristics of tropical and subtropical legume crops, lima beans, chick pea and guar was possible to grow in Korea, but the lentils needed to reconfirm. total sixty eight accessions of lentil were used in this study, 58 accessions of them can be harvested and 29 accessions of which can be obtained enough yield for evaluation. The agricultural characteristics of tropical and subtropical legume crops do not differ much from those of control common bean. In addition, legume crops were excellent than common bean in physicochemical properties. Therefore tropical and subtropical legume crops, chick pea, guar and lima bean seem to be useful to utilize in domestic. *Corresponding Author: Tel , cym0421@korea,kr 166

223 PD-72 Wan Woo Yeam 1, Hye Jeong Kim 1, Hyun Suk Cho 1, Jin Sol Park 1, Jae Yong Han 1, Cha Young Kim 2, Young-Soo Chung 1* 1 2 Anthocyanin, flavonoid-based natural pigment, is contained in flowers and fruits of plants. Anthocyanins are anti-oxidant and anti-cancer agents when ingested by humans. IbMYB1a gene derived from Ipomoea batatas, is a transcription factor that promotes ezymatic activity of anthocyanin biosynthetic pathway. To use soybean as antioxidant, transgenic plant was created by inserting IbMYB1a gene into soybean genome along with over-expression promoter. Transgenic plant (T 0 ) was produced by using modified half-seed method, and PCR analysis of T-DNA was performed to confirm the insertion of IbMYB1a gene. Among transgenic T 1 plants, line #6 plant showed dark purple color. Moreover, high level of gene expression was verified in line #6 plant by using RT-PCR. Leaves of the transgenic plants were analyzed by HPLC. As a result, anthocyanin content of the line # 6 was 160-times higher than wild type soybean. Transgenic line #6 plant (T 1 ) was grown to identify the phenotypic change. Line #6 plant was strongly purple, but leaves were dry and consequently failed to produce flowers. In addition, we are identifying phenotype by germinating line #7 plant seeds, which anthocyanin content is measured 14-times higher than wild type soybean. *Corresponding Author: Tel , chungys@dau.ac.kr PD-73 Geum Sol Kim 1, Cheol Seong Jang 1* Some toxic plants are morphologically very similar to edible plants. Accidents of poisoning often occur by confusing wild toxic plants as edible plants. In fact, due to a similar appearance between the two species, 42 patients have occured in the past five years. Caltha is one of the plants with poison. The anemonine, which is the cause of the toxicity of the Caltha, has a strong irritating effect, which causes nausea, vomiting and diarrhea and stimulates the kidneys to cause hematuria and proteinuria. Therefore, we needed to develop a discrimination method that distinguishes between Ligularia and Caltha..Seeds of Ligularia and Caltha were obtained from the National Institute Of Biological Resources. We used chloroplast genes such as matk, ndhf, and rbcl to develop species-specific primers. The sensitivity of primer sets are assessed serially ten-fold diluted of total DNA and efficiency analyzed in each primer sets using the regression test. The efficiency of each primer set was within %. A linear correlation (R 2 >0.99) were obtained between the crossing point values and log DNA concentration. We determined the Ct value of 10pg of the target species as the cut-off line, and that the Ct value of all non-target species amplified more than 2 cycles lather than this cut-off line. We developed species-specific primer sets that it could be as a useful tool to distinguish toxic plants and edible plants. *Corresponding Author: Tel , csjangl@kangwon.ac.kr 167

224 PD-74 Chong Chen and Jae Bok Heo * The Nug protein family is an important part of GTPases which involves in ribosome biogenesis, cell proliferation, and cell growth. Recently, We characterized a rice (Oryza sativa) nuclear/nucleolar GTPase 2 (OsNug2), which belongs to the YlqF/YawG family of GTPases, playing a role in maturation of pre-60s ribosomal subunit. Yeast two-hybrid screening, using OsNug2 as bait, was carried out to find out potential interaction factors, and rice serine/threonine kinase 1 (OsSTK1) was identified as a candidate. When recombinant OsSTK1 was added into OsNug2 assay reaction mixtures, OsSTK1 increased the intrinsic GTPase activity of OsNug2 significantly. The kinase assay of OsSTK1 in vitro reveals that, OsSTK1 strongly phosphorylated serine 209 of OsNug2. Yeast complementation test result in a GAL::OsNug2(S209N) mutant-harboring yeast strain, exhibited a growth-defective phenotype on galactose medium at 39 C, divergent from that of a yeast strain harboring GAL::OsNug2. The intrinsic GTPase activity of mutant OsNug2(S209N) was similar to that of OsNug2, but could not be enhanced upon weak binding of OsSTK1. Our findings reported here suggest that OsSTK1 functions as a positive regulator of OsNug2 through binding and enhancing the GTPase activity of OsNug2, and that the phosphorylation of serine 209 of OsNug2 is essential for the complete function of OsNug2 in ribosome biogenesis. *Corresponding Author: cc @gmail.com PD-75 Ji Yul Lee *, Gi hoon Kim, Cheol Choi Abstract Domesticated apple (Malus domesitica Borkh.) is one of the most widely cultivated and loved temperate fruit crops in world. One of domesticated apple Fuji (Ralls Genet Delicious) apple is favorite apple cultivar to Koreans has been very famous after it was promoted in 1958, Japan. But, Fuji apple has genetic problem that cause restriction high quality fruit production. To solve these 'Fuji' apple s problems, various and numerous somatic mutation cultivars were bred and selected. These somatic mutation cultivars are classified with three major group of fruiting spur group, early season group, and coloring group. However, 'Fuji' and its somatic mutation cultivars have genetic similarity and not much different phenotype too. For these reason, it has hard to the identification of between 'Fuji' and its somatic mutation cultivars. Therefore, in this research, we identify variety genetic diversity different between Fuji and its somatic mutation cultivars by using Re-sequencing. We used Re-sequencing using Golden Delicious apple genome to reference genome. Insertion/deletion (InDels) and Single nucleotide polymorphism(snp) of Fuji apple and its somatic mutation cultivars were discovered. we selected and designed the Indel marker and Allele specific PCR (AS-PCR) primer out of several markers considering reproducibility and resolution power. And data from result had selected and had converted into InDel markers and allele specific PCR primers to used it for identify between 'Fuji' and its somatic mutation cultivars. And, the results obtained with InDel markers and AS-PCR primers which distinguish Fuji and its somatic mutation cultivars will be advanced that helpful to Fuji somatic mutation cultivar breeders. *Corresponding Author: Tel , roche0302@naver.com 168

225 PD-76 Ju-Pyo Hong, Hea-Young Lee, Na Young Ro, Jin-Kyung Kwon and Byoung-Cheorl Kang * Pungency is an unique trait that only pepper (capsicum spp. genus) has. Pepper s pungency is originated from its secondary metabolite, capsaicinoids that are valuable for industrial and medical purpose. Although several studies have identified loci that control capsaicinoids contents, still it is difficult to utilize on a breeding program and shows low efficiency since the trait controlled by QTL. Combining with high density SNP marker and statistical prediction model, genomic selection (GS) has been proposed as a solution to limitations of QTL mapping in plant breeding. To construct GS model of capsaicinoids contents, 351 pepper core collection were used to quantify its pungency level during 2 years. Genotype was obtained from genotype by sequencing (GBS) data which provides 168,714 SNPs. Four capsaicinoids compound (capsaicin, dihydrocapsicin, capsiate, dihydrocapsiate) were extracted from the mature fruits and quantified its pungency level by HPLC analysis. Through GBS genotype data, we conducted population stratification based on principle component analysis (PCA) in which pepper core collection divided into four sub-populations. Through HPLC analysis, we obtained overall capsaicinoids level distribution sorted by species. To Compare these two data set, we plotted a capsaicinoids compound level heatmap with phylogenetic tree that shows correlation between genotype and phenotype. Results of this experiment, ultimately, could be used to construct an accurate genome-wide selection model as an explanatory variable. Furthermore, it could provide information in which breeders can choose specific core collection for pungency trait pre-breeding. *Corresponding Author: Tel , bk45@snu.ac.kr PD-77 Inkyu Park 1, Sungyu Yang 1, Wook Jin Kim 1, Pureum Noh 1, Hyun Oh Lee 2, Byeong Cheol Moon 1* 1 2 Ipomoea species (morning glories) are economically valuable as horticultural species and scientifically valuable as ecological model plants to investigate mating systems, molecular evolution, and both plant-herbivore and plant-parasite interactions. Furthermore, the dried seeds of I. nil or I. purpurea are used in Korean traditional herbal medicines. In this study, chloroplast (cp) genomes were sequenced from six Ipomoea species, namely, I. nil and I. purpurea and, for the first time, I. triloba, I. lacunosa, I. hederacea, and I. hederacea var. integriuscula. The cp genomes were 161, ,750 bp in length and exhibited conserved quadripartite structures. In total, 112 genes were identified, including 78 protein-coding regions, 30 transfer RNA (trna) genes, and four ribosomal RNA (rrna) genes. Comparison of the six Ipomoea cp genomes revealed locally divergent regions, mainly within intergenic spacer regions. In addition, the protein-coding genes accd, cema, and ycf2 exhibited high sequence variability and were under positive selection (Ka/Ks >1), indicating adaptive evolution to the environment within the Ipomoea genus. Phylogenetic analysis of the six Ipomoea species revealed that these species clustered according to the APG IV system. We uncovered high-resolution phylogenetic relationships between Ipomoeeae. Finally, indel markers (IPOTY and IPOYCF1) were developed for the discrimination of the important herbal medicine species I. nil and I. purpurea. The cp genomes and analyses in this study provide useful information for taxonomic, phylogenetic, and evolutionary analysis of the Ipomoea genome, and the indel markers will be useful for authentication of herbal medicines. *Corresponding Author: Tel , bcmoon@kiom.re.kr 169

226 PD-78 Sungup Kim *, Sovetgul Asekova, Eunyoung Oh, Jung In Kim, Myoung Hee Lee, Suk-Bok Pae, Tae Jung Ha, Do-Yeon Kwak Sesame is cultivated in both in the field and in the greenhouse followed postharvest of vegetables and fruits. Sesame seeds contain 50% oil, 25% protein and 0.5% lignan content. It is considered as valuable vegetable oil, easy to extract as roasted, as well as refined unroasted oil. In this study, we evaluated the variation of seed quality of sesame grown in field and greenhouse conditions. We analyzed crude fat, crude protein, lignan contents and fatty acid composition of twelve cultivar Ansan, Daheuk, Dodam, Galmi, Hwangbaek, Jinki, Jinyul, Kangheuk, Pyeongan, Sangbaek, Seodun, Yangbaek. The cultivar Seodun showed the highest crude protein and oleic acid composition of 31.2% and 49.2%, respectively. The cultivar Sangbaek showed the highest crude fat of (52.4%) content whereas, Yangbaek showed the highest lignan content (6.31mg/g). Estimated crude protein, crude fat, lignan and fatty acid contents of sesame seeds were varied among cultivars and influenced by environment. The crude protein, lignan and linoleic acid contents were relatively high whereas, crude fat and oleic acid contents were slightly low when grown in greenhouse condition. The results showed that sesame seeds grown in the greenhouse had higher quality than those grown in the field. *Corresponding Author: Tel , PD-79 Sung Jin Choi *, Junkyoung Choe, Jae-eun Oh, Sung-Hwan Jo The devolopment of Next Generation Sequencing (NGS) technique and bioinformatics skills have led a daramatical increase of genomic data. This information can be used to develop comprehensive and precise molecular markers for breeding since thousands upon thousands of SNPs are extracted from NGS dataset. To use of these data for molecular breeding the linkage of the information to phenotype data is an essential requirement, however phenotype information seem relatively insufficient to utilize genome information in agriculture. Thus, we developed a new module for management of phenotype data in the existing web-based database, Phenome and Genome Database for Breeding (PGDB) that can search molecular markers associated with traits of interest. At present, the types of phenotypes are divided into a total of 42 traits of tomato, and user can input these data in bulk through CSV files or input them individually for each item using web interface. In addition, phenotype images are also stored in it. In the future, we will link seed numbers commonly used among related researchers for clear management of these information. These features from this study can allows seed company and breeders to manage phenotype information of their seeds and then could be extended further to various crops. *Corresponding Author: Tel , jc@seeders.co.kr 170

227 PD-80 Chang-Ho Eun 1*, In-Jung Kim Citrus fruits are well-known as an important dietary source of nutrients and health-promotion. It is widely distributed in the tropical and subtropical regions in the world and also Jeju island in south korea. Several studies have reported anti-oxidant activity and phenolic compounds in commercially available citrus species but not yet investigated for various stages during fruit development and tissues. Aging of human skin is an inevitable biological phenomenon by intrinsic and extrinsic factors as accumulation of reactive oxygen species (ROS) which leads to the activation of elastase, collagenase and hyaluronidase. So far, the extract from several plant, such as rice, alfalfa and tea etc, have been investigated for elastase, collagenase and hyaluronidase inhibitory activities, but anti-aging assay from the extract of citrus fruit have not been elucidated yet. In this present study, anti-oxidant activity and total phenolic/flavonoid content of Citrus unshiu extract during fruit development and tissues were evaluated as well as inhibitory activities of elastase and collagenase. And we also investigated the total carotenoid and chlorophyll a/b content *Corresponding Author: Tel , mong6908@gmail.com PD-81 Minjeong Park, Dogyeong Lee Joung-Ho Lee, Koeun Han, Byoung-Cheorl Kang The pungent flavor of chillies is induced by capsaicinoids found only in the genus Capsicum. apsaicinoid biosynthesis occurs in the placental epidermis cells, secreted towards the outer cell wall, and finally accumulate blisters located on the placenta surface. However, extremely pungent pepper such as C. chinense Trinidad Moruga Scorpion, extreme character of chilli is owing to a volatile phenolic amine Capsaicin, a molecule which is responsible for the pungency of chilli peppers and found mainly in the placenta as well as fleshy tissue of the fruit. The goal of the present study was to identify putative genes involved in controlling capsaicinoid biosynthesis in the pericarp by analyzing changes in global gene expression patterns. RNA-seq was used to analyze the expression profiles in the pericarp tissue over three developmental stages of three Capsicum cultivars with different capsaicinoid content: a highly pungent cultivar C. chinense Trinidad Moruga Scorpion, a pungent cultivar C. chinense Habanero, and a non-pungent C. annuum Early Calwonder (ECW). Changes in gene expression patterns were determined by comparing two cultivars at each developmental stages: 18 days after pollination (DAP), 34 DAP, and 45 DAP. We identified genes differentially expressed in the pericarp of Scorpion compared to Habanero 1,437, and 1,717, and 676 were up-regulated while 1,195, 1,313, and 654 were down-regulated at 18, 34, and 45 DAP, respectively. Additionally, to functionally categorize DEGs, these DEGs were mapped to terms in the KEGG database. Furthermore, we analyzed the expression patterns of 12 genes of known function in the capsacinoid biosynthesis pathway. Multiple genes including Pun1, pamt and KAS were up-regulated in the pericarp of Scorpion. Increasing capsaicinoid content is an important objective of pepper breeding. Our comprehensive transcriptional overview will be helpful for revealing genes involving capsaicinoid biosynthesis in the pericarp, and thus enhancing the capsaicinoid content in the whole fruit. *Corresponding Author: Tel. 02) , bk54@snu.ac.kr 171

228 PD-82 Hye Jeong Cho *, Yu Jin Kim, Gi Hoon Kim, and Cheol Choi To overcome genetic weakness of Fuji leading pale coloration, its enhanced somatic mutants were widely cultivated including Benishogun. In order to examine whether apple peel phenotype difference between cultivars was due to expression of anthocyanin and carotenoid biosynthesis related genes, RNA was isolated from fruit skin samples during six developmental stages and cdna was synthesized immediately. And then, that was analyzed by quantitative real time PCR. The results generally showed that the expression level of most genes were increased at maturity stage. But there was a significant difference between Fuji and Benishogun. In case of eight anthocyanin involved genes, MdCHI, MdF3 H, MdMYB10 and MdGST and about three carotenoid involved genes, MdZISO, MdCRTISO and MdLCY-ε highly expressed in Benishogun than Fuji. These observations may have increased the accumulation of anthocyanin and carotenoid in Benishogun inducing diverse apple skin phenotype. *Corresponding Author: Tel , hyejeong725@naver.com PD-83 A Reum Han *, Hyun Wook Shin, Gi Hoon Kim, and Cheol Choi Fruit breeding is imperative that new cultivars are selected to have outstanding consumer appeal. To improve fruit quality, it is necessary to confirm association between genetic and fruit traits useful for fruit breeding. Genome-wide association study(gwas) that links molecular genetic information with phenotypic information is a method that is being used in various fields today, and it can be used to search for genes that are related to the target trait of the genome. In this study, using GBS(genotyping-by-sequencing) data of NGS technology generated from 308 apple germplasm, GWAS was performed to indentify SNPs associated with fruit traits. High association level SNPs were confirmed on chromosome 9 about skin color and were identified on chromosome 16 about acidity. The results of this study will can be proposed candidate gene and marker including SNP associated with fruit traits. Also, application of molecular markers developed through phenotypic and genomic analysis to apples mating population will be able to select useful individuals and used as the basis of apple molecular breeding. *Corresponding Author: Tel , har0903@naver.com 172

229 PD-84 Tae-Ho Lee *, Jae-Hyeon Oh, Do-Wan Kim, Dong-Jun Lee, Byung-Ohg Ahn With the fast development of NGS technology and the decline in prices, various genotyping methods based on sequencing have been developed. In particular, Genotyping-By-Sequencing (GBS) technology has been used in genotyping of agricultural organisms since 2011 and has become a useful technology in agricultural plant research since the announcement of the TASSEL-GBS pipeline in The GBS pipeline is divided into the referenced-base method and the de novo method, depending on whether the reference is used. However, many agriculturally important crops have large genomes due to polyploidy and a huge number of repeat sequences but have no reference genome. At present, Stacks and UNEAK support the de novo method. Studies have shown that the two pipelines work relatively well in diploid and medium-sized genome plants, but the performance of the pipelines for the large genomes is not clear. In this study, we tested the pipelines with a bread wheat genome (Triticum aestivum) which has large hexaploidy genome(15.3 Gb). The results show both pipelines have a proper performance for the large genomes but need to be improved. *Corresponding Author: Tel , thlee0@korea.kr PD-85 Tae-Ho Lee *, Cho-Yil Cho, Jae-Hyeon Oh, Do-Wan Kim, Byung-Ohg Ahn The genome sequence is a base of the modern breeding technologies such as genomic selection. Notably, it is more important to obtain high-quality genome sequence for a crop which has complex genome structure. Up to date, to improve a genome assembly quality, there were many tries to use information of synteny generated by speciation and a (whole) genome duplication which are general evolutionary events in plants. However, the most usages of synteny information are determining of order and orientation of contigs(scaffolding), but there was no noticeable report in a determination of the length of a junction between contigs(gap). Herein, to address the possibility that the synteny is used to determine gap length, we identified synteny regions between 23 representative plant genomes and compared the features such as the number of genes and the length. The results such as a distribution of the length difference show that the information can be used to improve a genome assembly. *Corresponding Author: Tel , thlee0@korea.kr 173

230 PD-86 Seunghye Park, Jung Heo, Min-Sung Kang, Soon Ju Park Apical shoots dominantly grow upright to maintain the top position until stems are fully mature or terminate to reproductive stems such as pedicel and floral stem. Plant stems have a critical time point to react to gravity or light vectors. In Solanaceae, shoot architecture has been remarkably diverged, developing sympodial (inflorescence) shoot carrying single flower to both sympodial shoot and inflorescence producing compound flowers. To understand this divergence, we hypothesize that the direction of sympodial growths were decided by maturation rates. In tomato, new sympodial shoot take place of main erect stem after primary shoot transition to inflorescence and terminate to flowers. Here we show tomato inflorescence could be shifted to sympodial shoot growth with erect growth in mutants, indicating inflorescence meristems can be reverted to vegetative status by the delayed maturation in the meristem. mc sp double and s single mutant developed inflorescence growing upright, which is similar that of S. peruvianum. Erect type inflorescences reverted to vegetative state were produced in sft, mc, and j background. Moreover, sft, j double produced only sympodial shoots carrying single flower, which is similar to the sympodial shoot of N. benthamiana. Interestingly, molecular state of inflorescence meristems indicated delayed maturation of the meristems in s mutant and sft mutant. Therefore, we suggest that sympodial shoot meristem and sympodial inflorescence meristem might have been evolved by variations in maturation state and activity of erect growth response on each young stem in Solanaceae. *Corresponding Author: Tel , sjpark75@wku.ac.kr PD-87 Chuan-Jie Zhang, Min Jung Yook, Hae-Rim Park, Do-Soon Kim * With increasing GM crops imports and exports worldwide, the concern of transgene flow and environmental risks from GM crops is growing. Particularly in Asia including Korea which has smallholder farming system, concerns about ecological impact of GM crops on non-target organisms, such as their closely-related relative species are high.. However, few studies about environmental risks of GM crops have been conducted under smallholder farming systems in Asia with diverse crops in co-existence. Thus, in this study, two-year field study was conducted to assess potential environmental risks deriving from transgenic flow glufosinate-ammonium resistant (GR) Brassica napus to its conventional relatives, B. napus, B. juncea, and Raphanus sativus under simulated smallholder field conditions in Korea. Screening with glufosinate-ammonium was conducted on F1 hybrids, and PCR analysis was also conducted to detect bar-specific gene introgression. The result showed that hybridization frequency ranged between 0.007% (75m) to 2.33% (2m) for B. napus, and 0.025% (16m) to 0.076% (2m) for B. juncea. No gene flow was observed to R. sativus. Results suggest that long-distance gene flow from GR B. napus to B. napus and B. juncea is unlikely, but gene flow can potentially occur between adjacent fields where the smallholder farming systems exist. Acknowledgement: This research was supported by Rural Development Administration, Republic of Korea (Project code PJ ; Project of LMO Environmental Risk Assessment Center). *Corresponding Author: Tel , dosoonkim@snu.ac.kr 174

231 PD-88 김홍식 *, 김현태, 강범규, 서정현, 신상욱, 박장환, 곽도연 콩이소플라본은건강기능성식의약소재로서중요한생리활성성분이다. 국산콩의소비촉진과수입산과의차별화를위해본연구에서는용도별국내육성주요콩품종에대한이소플라본함량을분석하고원료곡자원선발및육종기초자료로활용하고자한다. 장류 두부용, 나물용등용도별구분된총 44 개품종을시험재료로국립식량과학원대구시험지에서 2016 ~ 2017 년수확한콩종자의이소플라본총량및조성별함량을 HPLC 로분석하였다. 이소플라본총량및조성별함량변이에대한품종간차이가인정되고, 시험년도는 daidzein 과 genistin 성분함량을제외하고유의성이인정되었다. 전품종의 2 년간평균이소플라본함량은 2,935 μg /g 이며, 용도별품종군평균함량은나물용 (3,850.4 μg /g) > 장류용 (3082.8) > 유색콩 (2345.8) > 올콩류 (1,298.6) 순이었다. 2 년간이소플라본함량에대한 44 개품종의순위상관은고도로유의하였으나 (r=0.92 *** ), 함량변이에대한품종과시험년도의상호작용효과도유의하였다. 나물용품종 소원콩 이전체공시품종에서함량이가장높았고 (5,226 μg /g), 신화 (4,956) 와 소록 (4,830) 이높은수준을나타내었다. 장류 두부용품종에서는 대풍 (4,962 μg /g) 이가장높고 대풍 2 호 (4,619), 새금 (4,602), 우람 (4,305) 순이었다. 반면, 태광콩, 새단백, 단백콩 등은 1,500 μg /g 미만으로낮았다. 생육기간이짧고대립인올콩류와안토시아닌함량이높고대립인검정콩품종은이소플라본함량이낮은경향이었다. 이소플라본조성성분비율은 malonyl 계배당체 ( 평균 2,437 μg /g) 가전함량의 83.0% 로가장높고 glucoside(9.5%), aglycon 비배당체 (5.4%), acetyl 계배당체 (2.1%) 순으로낮았다. 검정소립콩 소청자 등을제외하고종자크기가작을수록이소플라본함량은증가하는경향이었다. 단기성콩을포함한품종별파종 ~ 성숙기까지의생육일수는이소플라본함량변이와유의한상관을보였으나 (r 2016 =0.40 **, r 2017 =0.44 ** ), 개화기 ~ 성숙기까지의생육일수는유의성이없었다. 품종별개화이후성숙기까지의기온이높을수록이소플라본함량은낮은경향을보여역의상관관계를나타내었다. 향후이소플라본함량이안정적으로높거나낮은품종을식품및산업소재화함으로서시장과소비자의다양한기능성신수요창출및지원이기대된다. * 교신저자 : Tel , kimhongs@korea.kr PD-89 Minjeong Park 1, Ju-pyo Hong 1, Jinhee Kim 2, Do-Sun Kim 2, Sun-yi Lee 2, Heayoung Lee 1, Koeun Han 1, Byoung-Cheorl Kang 1* 1 2 The cultivated strawberry, Fragaria x ananassa, is an allo-octoploid species (2n = 8x = 56) with an estimated genome size of Mb. Although strawberry is one of the important vegetable crops in Korea, the complex structure of the genome prevented research in genomics of strawberry. However, advances of genomics in Fragaria has been made by releasing genome sequences of diploid F. vesca and octoploid F. ananassa. In this study, we constructed linkage maps of strawberry families using single nucleotide polymorphism (SNP) markers discovered by genotyping-by-sequencing (GBS). Three F. ananassa varieties were used for development of two F2 cultivated strawberry families: Benihoppe x Chandler ( BC ) and Benihoppe x Doyonoka ( BD ). We compared the SNP discovery results when utilizing two reference genomes. In BC population, a total of 192,649 and 2,245 SNPs were obtained for F. vesca and F. ananassa respectively. In BD population, a total of 165,893 and 748 SNPs were obtained for F. vesca and F. ananassa respectively. A linkage map for BC population consisted of 25 linkage groups possessing 145 high-quality SNPs identified from F. ananassa reference genome and total map length was 686 cm. 134 SNP markers obtained by mapping to F. vesca reference genome were used for a linkage map of BD population and the total length of seven linkage groups was 1,234 cm. In addition, we showed the complementary application of two reference genome according to SNPs to be analyzed. This SNP-based linkage maps will be helpful for the genetics and breeding of allo-octoploid strawberry. *Corresponding Author: Tel , bk45@snu.ac.kr 175

232 PD-90 Do Yoon Hyun *, Muhammad Rauf, Sejong Oh, Myung-Chul Lee, Yu-Mi Choi, Sukyeung Lee 메밀은마디풀과 (polygonaceae) 의메밀속에속하는일년생초본으로야생종을포함하여 20 여종이확인되고있다. 현재재배되고있는메밀재배종은보통메밀 (Fagopyrum esculentum Moench) 과쓴메밀 (Fagopyrum tataricum Gaertn., 타타리메밀 ) 등두종이주류를이루고있으며우리나라에서는보통메밀이주로재배되어왔다. 본연구는농업유전자원센터에서보유중인메밀유전자원 183 점을봄, 여름에파종하여재배시기별생육특성과플라보노이드함량을비교하기위해수행되었다. 봄, 여름파종은각각 2017 년 4 월 14 일과 8 월 17 일에전주소재농업유전자원센터포장에하였으며개화기, 성숙기, 주경절수, 총분지수, 백립중등 5 개농업형질을조사하였다. 봄파종시개화기는대부분 5 월 25 일전후하여파종일로부터약 40 일이소요되었고성숙기는 6 월말부터 7 월중순까지분포하였다. 여름파종시는 9 월 15 일전후개화하여파종후 일이소요되었고 10 월말에성숙하였다. 종자생산과관련된주경절수와총분지수는봄파종과여름파종에서많은차이를나타냈으며봄재배시영양생장이왕성하여가을재배보다많은주경절수와총분지수가조사되었다. 그러나백립중에서는여름파종이봄파종보다무거운경향을보여주경절수, 총분지수와백립중간부의상관관계가있었다. 일반메밀유전자원을국내수집지별로나누어조사한결과주경절수와총분지수는봄, 여름파종모두에서전남수집자원이가장많았으며, 강원, 경남, 경북, 전북, 충북수집자원과유의성있는차이를나타내었다. 강원, 충북수집자원은제일낮은주경절수와총분지수를보였으나백립중은가장높게조사되었다. 성숙종자의플라보노이드함량을조사한결과봄재배자원은 0.22 mg/g 을나타내었고가을재배자원은 2 배높은 0.44 mg/g 의함량을보여가을재배시더높은플라보노이드를축적함을알수있었다. 수집지역별로는전남수집자원이봄, 가을재배모두에서가장높은플라보노이드함량을보였으며, 봄재배시지역간유의성있는함량차이가나타났지만가을재배에서는비슷한경향을보여봄재배가메밀유전자원의플라보노이드함량차이에더많은영향을미치는것을확인하였다. *Corresponding Author: Tel , dyhyun@korea.kr PD-91 조준현 1*, 강주원, 이지윤, 조수민, 권영호, 이샛별, 김태헌, 송유천, 박동수, 고종민, 이종희 1 일품벼의 MNU 돌연변이처리로육성된설갱벼의분상질 (fluory) 에관여하는유전분석및 DNA 연관마커를개발하고자정상품종인새일미벼와설갱벼가교배된 F1 식물체에서채종한 F2 종자의현미를내어유전분석을위한표현형을조사하였다. 분상질과정상의각각 35 개, 115 개로서 1:3 으로분리하였으며 (X 2 =0.222, p=0.637), 1 개의열성유전자에의해지배됨을확인하였다. 또한설갱의분상질유전자지도작성및연관마커를탐색을위해 370 개의 KASP 마커이용하여모부본다형성을분석하였다. 다형성을보인 153 개마커로연관분석용 Frame map 을작성하였 ek. 또한열성형질인분상질식물체 12 개체를선발하여 recessive class analysis 방법으로유전자형을분석한결과 3 번염색체의단완에 KJ03_017(6.0Mb) ~ KJ03_029(12.0Mb) 사이의분자마커와연관성이높은것을확인하였다. BC1F2 집단으로분자마커와표현형의연관분석을수행한결과 3 번염색체 RM218(8.3Mb) 와 KJ0329(12.3) 사이설갱벼의분상질유전자가존재하는것을확인하였다. 또한 3 번염색체 10.1Mb 에위치한 Indel 마커 RD03-25 는분상질유전자와조환가가 1.2% 로밀접히연관되었음을확인하였다. 이는현재까지알려진분상질유전자와다른새로운위치에존재하고있어이유전자를 flo14(t) 로명명하였다. * 주저자 : Tel , hy4997@korea.kr * 교신저자 : Tel , ccriljh@korea.kr 176

233 PD-92 Kyung Eun Lee, Sang Gu Kang * Rice Purple pericarp (Prp) and the Purple leaf (Pl w ) traits were analyzed. Rice Prp trait is controlled complementary gene interaction of Pb and Pp. The Pl w trait is resulted by the epistatic interaction of Pl gene with a complementary gene. Dominant Pb allele encodes OsB1 protein and expressed in seed specific mode for purple color determination in seed pericarp. The Pl gene is expressed OsB2 protein in purple leaf. BothOsB1 and OsB2 contain a helix-loop-helix (HLH) domain for transcription factors. However, it is still ambiguous that both genes are involved in expression of anthocyanins in both seed and/or leaf. We identified Pl w trait was governed by a dominant Pl allele encoding a functional OsB2 protein molecule for purple leaf only. In molecular level, recessive alleles of these two traits were caused by frame-shift mutations. In phenotypes, we identified that purple color phenotypes in different tissues either seed pericarp or leaf were determined by the gene expression patterns of Pb and Pl genes. Differentiated gene expression of Pb and Pl were resulted in differential accumulation of anthocyanin in specific tissues. Because these two genes having very similar domain structures, it may be possible share the same complementary gene in genetics. Here we defined that the two genes are high homologous but different manners in tissue specific regulation resulting in purple seeds(prp) was produced by Pb gene but purple leaf (Pl) was produced by Pl gene. *Corresponding Author: Tel , kangsg@ynu.ac.kr PD-93 Yeeun Jang 1, Woojong Jang 1, Hyun-Jin Koo 1, Hyunjo Koo 1, Vo Ngoc Linh Giang 1, Min Jung Kim 1, and Tae-Jin Yang 1* 1 Panax ginseng is a valuable medicinal plant, as containing various ginsenosides which have many benefits to human health. A lot of pharmacological studies for the herb have been carried out for a long time, however, genomic studies are fewer because of unusual slow growth and complex genome characteristics. To construct molecular breeding basis for ginseng, genetic researches using various genetic resources are demanded. We analyzed various 119 ginseng breeding lines using genotyping by sequencing (GBS) technique to elucidate genetic variations and develop useful markers. Through mapping to reference sequence, 173,138 SNPs were identified. Among them, high quality of 1,229 SNPs (0% missing data, 5 minimum depth) were used for analysis of population structure. As a result, the accessions were clustered into four populations. Also, phylogenetic analysis showed that 14 cultivars were evenly distributed in various groups. It indicated that ginseng breeding lines have abundant genetic diversity and these variations could be used as valuable information for further breeding researches. Consequently, we identified abundant genome-wide SNPs and elucidated population structure using the 119 ginseng accessions. These results will be used for genome wide association studies about diverse agricultural traits and for development of useful markers which applicable to other ginseng genetic resources for breeding. This work was carried out with the support of "Cooperative Research Program for Agriculture Science & Technology Development (PJ )" Rual Development Administration, Republic of Korea. *Corresponding Author: Tel tjyang@snu.ac.kr 177

234 PD-94 On-Sook Hur *, Na-Young Ro, Jung-Sook Sung, Jae Eun Lee, Ho-sun Lee, Sok Young Lee, Awraris Derbie Assefa, Ju-Hee Rhee This study evaluated the content variation of major carotenoids in 250 tomato germplasm also with agriculatural fruit characteristics to search for good quality breeding resources. One hundred and three accessions among 250 accessions using in this study are wild species including Solanum pimpinellifolium, S. habrochaites, S. peruvianum. The number of Solanum lycopersicum var. lycopersucum and S. lycopersicum var. ceraciforme are 101 and 46, respectively. Three individual carotenoids, lutein, lycopene, and β -carotene were quantified using fully matured tomato fruits at harvest using HPLC. The lutein content of 250 tomato germplasm showed a variation ranging from nt(not detected) to 9.3 mg/100g, DW. The lycopene contents were varied from 19.3 to mg/100g, DW. The content of β -carotene was evaluated from 19.2 to mg/100g, DW. The nine accessions including IT could be selected as potential high-lycopene resources over 500 mg/100g, DW. IT showed the highest soluble solid content with 14.0±0.6 o Brix. These accessions could be used as potential breeding resources with respect to contents in sugar or functional chemicals, and further research is needed for the antioxidative and anticancer activities. Keywords: carotenoid, tomato, HPLC, lycopene, β -carotene, lutein *Corresponding Author: oshur09@korea.kr; Tel.: ; Fax: PD-95 Mi Jeong Jeong *, Joo Yeol Kim, Soo In Lee, and Jin A Kim We previously reported thatsound wave delays tomato fruit ripening by altering the expression of gene in ethylene biosynthesis. ). Here, we evaluated the molecular mechanism underlying this delaying fruit ripening by performing RNA-sequencing analysis of tomato fruits at 6 h, 2 days (d), 5 d and 7 d after 1 khz sound vibration treatment. Bioinformatic analysis of differentially expressed genes and non-coding small RNAs revealed that some of these genes are involved in plant hormone and cell wall modification processes. Ethylene and cytokinin biosynthesis and signalling genes were downregulated by sound vibration treatment, whereas genes involved in flavonoid, phenylpropanoid and glucan biosynthesis were upregulated.our results indicate that sound vibration helps delay fruit ripening through the sophisticated regulation of coding and non-coding RNAs and transcription factor genes. On the other hand, as the quality of life has improved, interest in secondary metabolites such as vitamin C, flavonoid and anthocyanin etc. We treated to alfalfa with various sound wave frequency, treatment time, and treatment period. Sound wave treated alfalfa sprouts had higher vitamin C content than non-treated sprout vegetables. To identify the mechanism of vitamin C content increase by sound wave, the expression pattern of vitamin C biosynthesis-related genes in sound-treated alfalfa sprouts was analyzed by qrt-pcr. *Corresponding Author: center1097@korea.kr 178

235 PD-96 Seung Woo Jin 1, Yeeun Jang 1, Sae Hyun Lee 1, Tae-Jin Yang 1* 1 Brassica oleraceae is one of the 10 most cultivated crops in the world. According to report, in Brassica oleraceae, the competitiveness of korean traditional breeding is world-class. With more advanced molecular breeding, Korea is expected to have global competitiveness in Brassica oleraceae. For development of SNP array, resequencing data of 44 accessions is mapped to reference genome. More than 3 million SNPs that is genotyped from more than 40 accessions are filtered. After filterings that are mean to enhance the accuracy of markers, 3446 SNPs that are from genic regions are filtered. Since paralogous seuqences can interfere fluorescent signal, SNPs with flanking sequences that have paralogous sequences in other regions are filtered. From 849 SNPs in single copy genes, 240 markers that are distributed throughout the genome to represent genome, are designed. After SNP array experiments, 192 markers that have high accordance rate with resequencing data are selected and these markers are utilized to 92 samples from LG farmhannong. 150 markers that can be used in MAB are selected finally with criteria of No call rate and clustering form of data points. Phylogeny tree that is drawn with 150 markers shows group 1 and group 2 that are consisted with cabbage and other Brassica oleracea subspecies each, are classified well and match with phenotype data well. These result shows these markers are working well and can be utilized to facilitate breeding period instantly. *Corresponding Author: Tel tjyang@snu.ac.kr PD-97 우관수 1*, 심동환 1, 강승미 2, 안지영 1, 이제완 1, 홍경낙 서로다른환경조건에서자란동일한채종원산소나무 (Pinus densiflora) 가계들이소나무재선충 (Bursaphelenchus xylophilus) 에인공적으로감염된후감수성및병진전에어떤차이를보이는지알아보고자본연구를수행하였다. 국립산림과학원산림생명자원연구부온실 (982 본 ) 과경남산림환경연구원포지 (1,079 본 ) 에서동일한병원성재선충균주로인공접종을실시한결과, 온실접종묘평균감염률은 91.5%, 진주포지평균감염률은 19.2% 를보여큰차이를나타냈다. 특히강원 13 은온실실험에서전체평균감염률에비해 17% 정도낮은감염률을보여다른가계에비해내병성이높은가계로확인되었으며강원 72 는감수성이가장높은가계로나타났다. 지역별로감염성에큰차이를보인주요원인으로는나지묘 vs. 포트묘라는차이점과포지 vs. 온실이라는묘목생장조건차이에따른것으로사료되며특히소나무재선충은 24~25 에서가장번식이왕성하므로인공접종후온도차가가장중요한 limiting factor 인것으로여겨진다. 두지역에서가계별감수성을비교해보면그순위가유사한것으로나타났는데이는소나무재선충에대한유전적감수성이환경차에도불구하고확연히나타남을알수있다. 성숙목에대해인공접종을실시하여내병성을보이는개체를선발하는것이가장이상적이긴하나선발하기까지걸리는시간적제약등여러가지문제로인해묘목인공접종을통해소나무재선충병내병성품종육성을추진하게되는데, 본연구결과로비추어묘목을이용하더라도나지묘와포트묘, 온실과포지간소나무재선충에대한감수성에큰차이가있음을전제로수행되어야할것으로본다. * 교신저자 : Tel , woo9431@korea.kr 179

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237 차세대바이오그린21사업 식물분자육종사업단 2018년 7월 13일(금) 제주 라마다플라자 호텔

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239 OE-01 Jong-Yeol Lee 1, You-Ran Jang 1, Jin Sun Kim 1, Susan B. Altenbach 2, Sun-Hyung Lim Gliadin proteins are a major component of wheat flour and important determinants of bread making quality, but also present significant health problems for consumers with celiac disease or wheat allergies. In the present studies, we have attempted to explain the biochemical and molecular bases of these compelex and unfavourable properties, and to develop strategies for their elimination. Firstly, to construct proteomic reference map, we extracted ethanol-soluble gliadin fractions from flour of the Korean bread wheat cultivar Keumkang. Proteins were separated by 2-dimensional gel electrophoresis (2-DGE) and individual spots were excised from gels, digested with chymotrypsin and subjected to tandem mass spectrometry (MS/MS). α -, γ - and ω -gliadins were identified as the predominant proteins in 31, 28 and one of 98 spots, respectively. Protein sequences were analyzed for specific epitopes related to celiac disease and food allergy. Secondly, to assign the individual gliadins to their chromosome encoded, we performed profiling of gliadins from Chinese Spring and its aneuploid lines missing chromosome 1 and 6 (and 6 short arms) using SDS-PAGE, A-PAGE, 2-DGE and MS/MS. In RP-HPLC, all peaks except one peak could be explained, but some bands (spots) could not be explained in SDS-PAGE, A-PAGE and 2-DGE. In particular, 11 out of 33 gliadin spots were not assigned in 2-DGE experiments. To confirm the identities of 11 unassigned spots, eleven individual spots were excised from 2-D gel, digested with chymotrypsin and subjected to MS/MS analysis. Unexpectedly, these spots were identified gamma and alpha gliadins of group 1 and 6 chromosome. The results may imply that certain regulatory mechanism may be involved in the regulation of gliadin protein expression. Thirdly, a new wheat mutant missing proteins encoded at Glu-B3 and Gli-B1 loci was discovered among double haploid lines obtained from a cross between the Korean wheat cultivars Keumkang and Olgeuru. Absence of the Glu-B3 LMW-GSs, w-5 gliadins and some γ -gliadins was determined by SDS-PAGE, 2-DGE and MS/MS. The deletion of Glu-B3 and Gli-B1 loci was also demonstrated using structure gene specific and loci specific DNA markers. Basic agronomic traits, protein content, dough mixing properties and bread loaf volume of DH20 and parental wheat cultivars were evaluated in field-grown wheat over a two year period. This mutant will be useful as a valuable resource for breeding efforts to reduce allergenic potential. Finally, we have attempted a transformation strategy to apply it directly to Korean commercial wheat cultivar 'Keumkang', along with the breeding strategy by obtaining the DH20 double haploid mutant lines. So far not only there is no transformation research on the Korean commercial wheat cultivar 'Keumkang', but also studies of systematic transformation method in Keumkang have rarely been progressed. Although the transformation efficiency is as low as ~1%, we succeeded to establish transgenic wheat. Our study contributes to a better understanding of how the heterogeneity of gliadins among wheat cultivars influences the immunogenic potential of the flour and also provides us with a valuable information to find and/or produce hypoallergenic wheat lines with reduced levels of harmful gliadin proteins using either breeding or biotechnology approaches. *Presenting author: Jong-Yeol Lee, jy0820@korea.kr 183

240 PE-01 Hyunhee Joo, Chae Woo Lim, Sung Chul Lee * Plants have evolved complex defense mechanisms to adapt and survive under adverse growth conditions. Abscisic acid (ABA) is a phytohormone that plays a pivotal role in the stress response, especially regulation of the stomatal aperture in response to drought stress. Here, we identified the pepper CaASRF1 (Capsicum annuum ABA Sensitive RING Finger E3 ligase 1) gene, which regulates drought stress tolerance via ABA-mediated signaling. We found that CaASRF1 contains a C3H2C3 type RING finger domain, which functions as an E3 ligase by attaching ubiquitins to the substrate target proteins. CaASRF1 expression was enhanced after exposure to ABA, drought, and NaCl. Loss-of-function in pepper plants and gain-of-function in Arabidopsis plants revealed that CaASRF1 positively regulates ABA signaling and the drought stress response. Moreover, CaASRF1 interacted with and was associated with degradation of the pepper bzip transcription factor CaAIBZ1 (Capsicum annuum ASRF1-Interacting bzip transcription factor 1). Contrary to CaASRF1 phenotypes, CaAIBZ1-silenced pepper plants and CaAIBZ1-overexpressing Arabidopsis plants exhibited drought-tolerant and drought-sensitive phenotypes, respectively. Taken together, our data indicate that CaASRF1 positively regulates ABA signaling and the drought stress response via modulation of CaAIBZ1 stability. *Corresponding Author: Tel , sclee1972@cau.ac.kr PE-02 Junsub Lim, Chae Woo Lim, Sung Chul Lee * Plants are sessile organisms; hence they constantly respond to environmental stress for their growth and development. The regulation of transpiration via stomata plays crucial roles in plant adaptation to environmental stresses, particularly drought stress. Lots of enzyme encoding genes involved in regulation of transpiration via modulating stomatal opening/closure. Here, we demonstrate that CaLEA5, encoding a late embryogenesis abundant protein, is critical regulator of transpirational water loss in pepper (Capsicum annuum). The expression level of CaLEA5 in pepper leaves was up-regulated after exposure to abscisic acid (ABA) and drought. The phenotype analysis showed that CaLEA5-silenced pepper and CaLEA5-OX plants exhibited reduced and enhanced drought tolerance, respectively, accompanied by an altered transpiration rate. Furthermore, the ABA sensitivity were significantly reduced in CaLEA5-silenced pepper, but increased in CaLEA5-OX plants compared with control plants, which resulted in opposite response to drought stress. Taken together, our data indicate that CaLEA5 positively regulates the ABA signaling and drought stress tolerance. *Corresponding Author: Tel , sclee1972@cau.ac.kr 184

241 PE-03 Woonhee Baek, Hyunhee Joo, Chae Woo Lim, Sung Chul Lee * Abscisic acid (ABA) is major plant hormone involved in regulating abiotic stress responses. Several studies have established that an ABA-signaling transduction pathway from ABA perception to response functions in plant cells. The group A PP2Cs constitute core components of ABA signaling, and they negatively regulate ABA signaling and stress responses. Recent studies have identified and functionally analyzed regulators of PP2C activity; however, the precise regulatory mechanisms remain unclear. In the present study, we used a yeast two-hybrid (Y2H) screening analysis to identify the DEAD-box RNA helicase RH8, which interacted with PP2CA in the nucleus. rh8 knockout mutants exhibited ABA hyposensitivity and drought-susceptible phenotypes characterized by high levels of transpirational water loss via reduced stomatal closure and decreased leaf temperatures. However, rh8/pp2ca double mutants showed ABA hypersensitivity and drought-tolerant phenotypes, indicating that RH8 and PP2CA function in the same ABA-signaling pathway in the drought stress response; moreover, RH8 functions upstream of PP2CA. In vitro phosphatase and kinase assays revealed that RH8 inhibits PP2CA phosphatase activity. Our data indicate that RH8 and its interacting partner PP2CA modulate the drought stress response via ABA-dependent signaling. *Corresponding Author: Tel , sclee1972@cau.ac.kr PE-04 Taewook Kim, Jemin Kim, Minsu Park and Chanseok Shin * Soybean growth is one of the most important traits for high yield breeding development. We selected normal and dwarf phenotype soybeans, which are F4 recombinant inbred lines from a cross between G. max (Peking, female) and soybean G. soja (male), and compared the gene expression associated with growth type. Whole transcriptome sequence was generated by Illumina HiSeq2500 sequencing platform. A total of more than thousand genes were identified to be highly differentially expressed between normal and dwarf mutant lines. The dwarf phenotype showed less expression of photosynthesis- and protein biosynthesis-related genes than normal phenotype. Interestingly, plastid-related genes are rarely expressed in the dwarf phenotype. Given that chloroplast, one kind of plastids, performs photosynthesis in plants, the dwarf phenotype seems to have low photosynthetic efficiency. Moreover, most of photosynthesis related genes such as thylakoid reaction and Calvin-Benson cycle were less expressed in dwarf phenotype than normal phenotype. Sucrose and other metabolites produced by the photosynthesis process have been found to interact with hormones to regulate and integrate many plant metabolic processes. We expect that these candidate genes will be useful for subsequent studies on plant growth. This work was supported by the Next-Generation BioGreen 21 Program (No. PJ ), Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , cshin@snu.ac.kr 185

242 PE-05 Juhyun Kim, Jaeil Kim, Hanul Kim, Eun-Jung Kim, Jihye Park, Annhee Im, and Kyuha Choi * Meiotic crossover recombination affects genetic diversity in population and is a critical tool for breeding. During meiosis, meiotic DSBs are repaired to reciprocal crossovers through class I and class II pathways. Anti-crossover factors such as FANCM, RECQ4 and FIGL1 were known to restrict crossovers in class II pathway dependent manner. However, the mechanism underlining how crossover number is limited to one to three along chromosome remains unexplored. We performed a high throughput genetic screening of higher crossover rate (hcr) mutants by using fluorescent seed-based system, enabling the measurement of crossover frequency in individual plants. We found that hcr mutants (hcr1, hcr2, hcr3) were new anti-crossover mutants by genetic analysis and deep-sequencing. We present characterization of hcr2 and hcr3 mutants, including mapping of them and their effects on crossover frequency in defined intervals along chromosomes. *Corresponding Author: Tel , kyuha@postech.ac.kr PE-06 Sunyoung Yang and Sangkee Rhee Nitrogen is an essential element for growth and reproduction of plant. In the past decade, it has been characterized in plants and many microbes that purine, a nitrogen-rich compound, is subject to enzyme-dependent degradation and the resulting products are ammonia and/or urea. In brief, the ureide pathway in plants is capable of utilizing urate, an early product from purine degradation, as substrate and undergoes sequential enzyme reactions to produce ammonia, glyoxylate, and/or urea. This ureide pathway has been suggested as one of the possible metabolic pathway for recycling nitrogen in plants. Unlike the downstream of purine catabolism by ureide pathway, the early biological events for purine degradation have been studied recently. In Arabidopsis thaliana, among those early events in purine degradation, guanosine deaminase (GSDA), a plant-specific enzyme, was characterized to be responsible for converting guanosine into xanthosine. Product xanthosine is then catalyzed into xanthine for further conversion into urate, a substrate for the ureide pathway. Presence of plant specific GSDA is very unique, because in most organisms guanine serves as a substrate of guanine deaminase (GDA) to produce xanthine. In order to understand substrate specificity of GSDA from Arabidopsis thaliana (AtGSDA) and its mechanistic features, we are undergoing structural studies of AtGSDA. In particular, AtGSDA is related in sequences to GDA but differs in its function. Specifically, they contain catalytic residues common for the cytidine/deoxycytidylate deaminase superfamily but their substrate specificities are unclear. Our analyses will provide structural basis for substrate specificity of AtGSDA and characterize enzyme mechanism in the early events of purine degradation in plants. This work was supported by Next Generation BioGreen 21 program of Rural Development Administration (Plant Molecular Breeding Center) of Republic of KOREA. *Corresponding Author: biotecher@snu.ac.kr 186

243 PE-07 Hwang-Bae Sohn *, Su-Jeong Kim, Su-Young Hong, Bon-Cheol Koo and Yul-Ho Kim Flowering time adaptation is a major breeding goal in soybean (Glycine max L.), yet they remain untapped due to the genetic linkage between the few useful alleles and hundreds of undesirable alleles. To investigate the genetic architecture of flowering time, a variation block analysis of flowering time was conducted with a diversity panel comprising 96 soybean cultivars and inbred lines grown in three agroecological conditions. In total, 7,087 variation blocks (VB) were mined by analyzing whole genome sequencing data of 96 soybean genotypes. Phenotypic associations with flowering time and seed yield were calculated in the panel over the year We identified 290 VB s associating with the onset of flowering among all soybean cultivars. Three clusters were inferred by STRUCTURE analysis, which is in good agreement with a neighbor-joining tree. In addition, soybean orthologs for a number of candidate genes for adaptation were detected, including soybean maturity locus E1. Further, backcross recombinant inbred lines (BC 2 F 3, Hwangkeum Ⅹ Daepoong ) exhibited significant variations in their onset of flowering, with a range of days due to obvious difference in E1 locus. Hence, VB analysis of candidate regions suggested that, selection of genes involved not only in flowering time but also in other trait may have high impact on diverse soybean cultivars. Furthermore, our study provides a valuable framework to improve the genetic resources of crop plants under changing environments. *Corresponding Author: Tel , misson7@korea.kr PE-08 Seungmee Jung 1, Jongchan Woo 2, Jeffery L. Caplan 3, Doil Choi 1, Savithramma P. Dinesh-Kumar 2, Eunsook Park 1,2* Chloroplasts play an essential role in producing immune signals in plant cells upon pathogen s attack beside their major role of using sunlight to provide energy for plant growth and development. Chloroplasts dynamically change their characteristics, proliferation, and positions in cells. Remarkable change of their morphology is to produce thin tubular structures, stromules, in cells faced to the various stress conditions. However, molecular function of stromules and the regulatory mechanism of stromule production remain elusive. Recently, we observed that active repositioning of the chloroplast close to nucleus occurs in the cells undergoing immune responses to bacterial and viral pathogens. Interestingly, numerous stromules were observed to extend toward the nucleus and attach their tips to the nucleus in the infected cells. Further study revealed that dynamic stromule induction is a part of plant immune responses and cytoskeletons in the infected cells might regulate the rapid changes in stromule length and position. In summary, we propose that stromule might provide a path to transfer signaling molecules from chloroplast to the nucleus as well as a driving force to translocate chloroplast body close to nuclei during plant innate immunity. *Corresponding Author: Tel , arabidopsis1004@snu.ac.kr 187

244 PE-09 Nam-Soo Jwa * Microbial pathogens have evolved protein effectors to promote virulence and cause disease in host plants. Pathogen effectors delivered into plant cells suppress plant immune responses and modulate host metabolism to support the infection processes of pathogens. Reactive oxygen species (ROS) act as cellular signaling molecules to trigger plant immune responses, such as pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) and effector-triggered immunity. In this review, we discuss recent insights into the molecular functions of pathogen effectors that target multiple steps in the ROS signaling pathway in plants. The perception of PAMPs by pattern recognition receptors leads to the rapid and strong production of ROS through activation of NADPH oxidase Respiratory Burst Oxidase Homologs (RBOHs) as well as peroxidases. Specific pathogen effectors directly or indirectly interact with plant nucleotide-binding leucine-rich repeat receptors to induce ROS production and the hypersensitive response in plant cells. By contrast, virulent pathogens possess effectors capable of suppressing plant ROS bursts in different ways during infection. PAMP-triggered ROS bursts are suppressed by pathogen effectors that target mitogen-activated protein kinase cascades. Moreover, pathogen effectors target vesicle trafficking or metabolic priming, leading to the suppression of ROS production. Secreted pathogen effectors block the metabolic coenzyme NADP-malic enzyme, inhibiting the transfer of electrons to the NADPH oxidases (RBOHs) responsible for ROS generation. Collectively, pathogen effectors may have evolved to converge on a common host protein network to suppress the common plant immune system, including the ROS burst and cell death response in plants. *Corresponding Author: Tel , nsjwa@sejong.ac.kr PE-10 Yeonhwa Jo, Jung Min Yoon, Kook-Hyung Kim and Hoseong Choi * Host proteins of Nicotiana benthamiana interacting with potato virus X (PVX) RNA stem-loop 1 (SL1) structure were identified by northwestern blot in previous study. Based upon previous results and N. benthamiana genome sequences draft, we identified two proteins, Potyviral CP-interacting protein 2a (NbCPIP2a) and Potyviral CP-interacting protein 2b (NbCPIP2b) that contains five amino acid differences. Electrophoretic mobility shift assay (EMSA) showed the interaction between PVX structures and two NbCPIPs. We observed that NbCPIPs bind to PVX SL1(+), SL1(-), and 3 SL. Especially, NbCPIP2a strongly interacted with PVX 3 SL structure than SL1(+) and SL1(-). Bimolecular Fluorescence Complementation (BiFC) was investigated to determine protein-protein interaction between two proteins and PVX viral proteins. By BiFC, we confirmed that NbCPIPs bind to only PVX coat protein (CP) in planta. Transient over-expression of NbCPIP2a and NbCPIP2b in N. benthamiana increased accumulation of GFP expressing PVX in local area. In protoplast experiment, PVX replication was also increased by transient overexpressed NbCPIP2a. Overexpression of NbCPIP2a positively affected systemic movement of PVX in N. benthamiana, whereas NbCPIP2b overexpression did not affect systemic movement of PVX. RNAi-mediated silencing experiment showed that PVX replication was hampered in NbCPIPs-silenced protoplast. These results suggest that PVX exploits a host protein NbCPIP2a than NbCPIP2b for local replication and systemic movement by interacting with SL cis-elements in PVX RNA and CP subunit. *Corresponding Author: Tel , bioplanths@snu.ac.kr 188

245 PE-11 Jinmi Yoon 1, Lae-Hyeon Cho 1, Wenzhu Yang 2, Yunfei Wu 1, Woo-Jong Hong 1, Charlotte Bureau 3, Soo Jin Wi 4, Tao Zhang 5, Rongchen Wang 6, Ki-Hong Jung 1, Ky Young Park 4, Christophe Périn 3, Yunde Zhao 5,7, and Gynheung An 1* Lateral roots are the most important component of the root system. Their functions include the acquisition of water and nutrient elements, anchorage of the plant, and biosynthesis of hormones. Although auxin plays a major role in their development and growth, the regulatory elements for its biosynthesis in lateral roots have not been well elucidated. Using rice, we identified an activation tagging line of a zinc-finger homeobox gene, ZHD2, which has longer-than-normal lateral roots. Because ZHD2 did not alter the density of lateral roots, it appears to function mainly in their emergence and elongation. When compared with the wild type (WT), the overexpressing plants absorbed nitrogen at a higher rate and grew better under a nitrogen deficiency. Knockout mutations of ZHD2 did not show any visible phenotypic alterations. However, double mutations of ZHD2 and ZHD1 reduced lateral root growth. To investigate how ZHD2 induces lateral root development, we performed transcriptome analyses of roots from activation tagging line ZHD2-D and the WT when sampled at the initiation stage. Genes for ethylene biosynthesis were up-regulated in ZHD2-D and those plants also had higher levels of ethylene than in the WT. Results from our ChIP assay suggested that ZHD2 interacts with the chromatins of SAM2 and ACS5. This implies that ethylene biosynthesis genes are controlled by ZHD2. We also generated transgenic rice plants expressing DR5::GUS in the WT and ZHD2-D. Histochemical staining indicated that expression of the DR5 reporter was greater in ZHD2-D than in the WT, especially at the root tip. This demonstrated that ZHD2 also affects auxin biosynthesis. These observations show that ZHD2 enhances lateral root development by influencing the biosynthesis of both ethylene and auxin. *Corresponding Author: genean@khu.ac.kr PE-12 Sangyool Lee 1, Sun Hyun Chang 1 and Geupil Jang 1* 1 Plastid-encoded RNA polymerase (PEP) plays an essential role in chloroplast biogenesis by regulating the transcription of chloroplast genes. Recent findings show that the PEP interacts with PEP-associated proteins to form PEP complex, and the formation of PEP-complex is key to controlling the activity of PEP to regulate expression of chloroplast genes and chloroplast development. FSD3 encoding iron superoxide dismutase is a PEP-associated protein. We found that FSD3 gene produces FSD3, a original form of FSD3, and FSD3S, a splicing variant of FSD3 by alternative splicing. Transcript level of FSD3 was higher than that of FSD3S in young leaves carrying well-developed chloroplasts, but lower in old leaves carrying senescent chloroplasts, gerontoplasts. Additionally, the enrichment of FSD3 and FSD3S transcripts was differently regulated in response to light, a key environmental factor governing chloroplast development. The transcript level of FSD3 increased in response to light, while the transcript level of FSD3S increased in response to darkness. The FSD3 and FSD3S transcripts encode proteins with identical N-termini, but different C-termini, and we found that the C-terminus of FSD3S exhibited higher hydrophobicity than that of FSD3. Further bioinformatics analysis revealed that the higher hydrophobicity of FSD3S is caused by a transmembrane domain, which is highly conserved in the TM domain proteins of plants. These findings suggest that FSD3S is a transmembrane proteins unlike FSD3S, and its function in chloroplast development might be different from that of FSD3. *Corresponding Author: Tel , yk3@snu.ac.kr 189

246 PE-13 고세리 *, 장지영, 이주영, 최규환 전통유전육종기술과첨단식물분자육종기술을융합하여고품질, 다수성기후변화대응신품종작물개발연구를수행하는차세대바이오그린 21 사업식물분자육종사업단은안정적이고지속적인식량자원확보를통한국가안보강화를이루고, 국내농생명바이오산업분야의활성화를위한우수연구개발성과활용방안을모색하고있다. 본연구에서는 1-2 단계 ( 년 ) 수행과제로부터도출된총 260 건의산업재산권과 86 건의농업형질개선효과가있는신품종작물품종보호출원성과들을종합분석하여, 성과확산및실용화추진대상으로유망산업재산권 52 건과슈퍼홍미, 고플라보노이드벼등우수신품종을 1 차선발하였다. SMART3 특허등급평가는국내등록유지특허를대상으로권리성, 기술성, 활용성부분을평가하여대량산업재산권성과물에서핵심특허를선발하는방법으로널리활용되고있다. 분석일현재기준국내등록특허로유지되고있는사업단도출특허 172 건은기술분류체계에따라분자육종소재개발연구기술 109 건, 형질도입 / 조직배양기술 26 건, 검출 / 진단기술 23 건이었고, 기능성물질생산시스템, 기능성소재응용기술, 그리고기타기술은각각 1, 9 건, 4 건으로분류되어, 식물분자육종사업단의최종연구목표에맞는연구개발성과물이도출되었음을알수있었다. 172 건중 SMART3 총점 BBB 등급이상의특허는 52 건으로, 분자육종소재개발연구기술 36 건, 그외기술은총 16 건이었고, 전반적으로권리성은높으나, 활용성이낮은기술이다수인탓에총점등급이하향되었음을알수있었다. 특히, 기후변화대응생물학적 / 비생물학적스트레스저항성분자육종소재개발기술과분자표지기반품종구별및병저항성품종선발둥다양한정밀분자육종기술이 BBB 등급이상의우수기술로다수포함되어있었다. 따라서, 해당분야의최신기술동향및시장동향조사를통한타겟시장분석과성과확산을위한수요기업을발굴하고, 수요기술과유사관련우수기술들의패키징및맞춤형기술홍보자료를제작및기술마케팅을통한성과확산을진행할예정이며, 연구자 - 사업단 - 수요기업간네트워크형성에기반한실용화추진체계구축은개별기술의기술이전시발생하던규모적한계와절차적한계를극복할수있을것이다. * 교신저자 : Tel , skohtn@naver.com PE-14 Boyoung Kim 1, Ankita Pandey 2, Hayoung Moon 2, Kee Hoon Sohn 2,3 and Cécile Segonzac 1* Ralstonia solanacearum (Rso) is a causal agent of bacterial wilt disease in Solanaceae crops and especially for pepper, which is second most important vegetable crop in Korea but no efficient genetic control has been identified. For tracking new genetic source of resistance in pepper, screening phenotypes of segregation by infection assay is used, however, Rso infection assay has limitations due to influence of the environment on disease development, host genome complexity and the genetic diversity inherent to Rso strains. To supplement the limitations, we develop a powerful methodology termed effector-assisted breeding to improve disease resistance in pepper against Rso. This approach relies on scoring the specific recognition of an avirulent effector by the matching resistance (R) gene which trigger hypersensitive response (HR), a form of programmed cell death that can be easily observed on pepper leaf. To establish single effector delivery system into pepper leaves, we conducted preliminary experiment using AvrBsT, a well-known avirulent effector from Xanthomonas euvesicatoria. We assayed two delivery systems, Agrobacterium tumefaciens-mediated transient transformation and non-pathogenic Pseudomonas fluorescens, (Pf0-1) which carry a functional type III secretion system (T3SS) in a commercial cultivar of pepper. As a result, the recognition of AvrBsT transferred by both single-effector delivery systems induced HR in commercial pepper leaves. Using this delivery system, we plan to identify avirulent Rso effectors and use them to screen the presence of matching R gene in recombinant inbred pepper lines. This approach will simplify and speed up the fine mapping of resistance loci, and the development of molecular markers tightly linked to bacterial wilt disease resistance in pepper. *Corresponding Author: Tel , csegonzac@snu.ac.kr 190

247 PE-15 Du-Hwa Lee, Seung Jun Park, Chang Sook Ahn, and Hyun-Sook Pai * Control of global translation activity is critical for cellular adaptation to fluctuating growth conditions and environmental stimuli, especially photosynthetic produced sugar level in plants. Therefore, photo-autotrophic plants adjust a valance of translation efficiency with cellular sugar availability through massive transcriptional modulation and phosphorylation of the translation machinery by Target of Rapamycin signaling. Here, we report that Arabidopsis thaliana MRF (MA3 DOMAIN-CONTAINING TRANSLATION REGULATORY FACTOR) family genes encode translation regulatory factors under TOR control, and their functions are particularly important in energy-deficient conditions. Four MRF family genes (MRF1 MRF4) are transcriptionally induced by dark and starvation (DS). Silencing of multiple MRFs increases susceptibility to DS and treatment with a TOR inhibitor, while MRF1 overexpression decreases susceptibility. MRF proteins interact with eif4a and co-fractionate with ribosomes. MRF silencing decreases translation activity, while MRF1 overexpression increases it, accompanied by altered ribosome patterns, particularly in DS. Furthermore, MRF deficiency in DS causes altered distribution of mrnas in sucrose gradient fractions, and accelerates rrna degradation. MRF expression, and MRF1 ribosome association and phosphorylation are modulated by cellular energy status and TOR activity. *Corresponding Author: planty@daum.net PE-16 Young Jeon and Hyun-Sook Pai * Ribosome Biogenesis GTPase A (RbgA) homologs are evolutionarily conserved GTPases that are widely distributed in both prokaryotes and eukaryotes. In this study, we investigated functions of chloroplast-targeted RbgA. Nicotiana benthamiana RbgA (NbRbgA) and Arabidopsis thaliana RbgA (AtRbgA) contained a conserved GTP-binding domain and a plant-specific C-terminal domain. NbRbgA and AtRbgA were mainly localized in chloroplasts, and possessed GTPase activity. Since Arabidopsis rbga null mutants exhibited an embryonic lethal phenotype, virus-induced gene silencing (VIGS) of NbRbgA was performed in N. benthamiana. NbRbgA VIGS resulted in a leaf-yellowing phenotype caused by disrupted chloroplast development. NbRbgA was mainly co-fractionated with 50S/70S ribosomes and interacted with the chloroplast ribosomal proteins cprpl6 and cprpl35. NbRbgA deficiency lowered the levels of mature 23S and 16S rrnas in chloroplasts and caused processing defects. Sucrose density gradient sedimentation revealed that NbRbgA-deficient chloroplasts contained reduced levels of mature 23S and 16S rrnas and diverse plastid-encoded mrnas in the polysomal fractions, suggesting decreased protein translation activity in the chloroplasts. Interestingly, NbRbgA protein was highly unstable under high light stress, suggesting its possible involvement in the control of chloroplast ribosome biogenesis under environmental stresses. Collectively, these results suggest a role for RbgA GTPase in chloroplast rrna processing/ribosome biogenesis, affecting chloroplast protein translation in higher plants. *Corresponding Author: planty@daum.net 191

248 PE-17 Jinsu Gil 1, Jaebok Lee 1, Sang Ik Park 1, So Hyeon Park 1, Kyoungmin Lee 1, Sung Cheol Koo 2, Ho Bang Kim 3, Yi Lee 2* Angelica species is a representative medicinal plants and it has been used in traditional herbal therapies. The Angelica species used in herbal medicine varies by country according to specific regulations, i.e. A. gigas Nakai in Korea, A. sinensis Diels in China, and A. acutiloba Kitagawa in Japan. Because of the similarity between the names, Danggui, they can be confused in the traditional medicine markets. In this study, twenty-four chloroplast insertion or deletion (CPInDel) markers were developed for the classification of Angelica species using chloroplast DNA sequences of A. gigas. Primer sets were designed from flanking sequences of the discovered InDel loci using CLC Main Workbench (version 6.8.4) with the following parameters: primer length = bp (Opt. 23 bp); GC% = 50 70% (Opt. 60%); Ta = C (Opt. 58 C); product size range = bp. Polymorphism and genotype analysis of thirteen Angelica species were performed using the CPInDel markers. The 24 CPInDel markers developed in this study could be used for genetic diversity analysis and classification of Angelica species. Acknowledgement: This work was carried out with the support of "Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ )" Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , leeyi22@cbnu.ac.kr PE-18 Hye-Jung Lee 1, Joon ki Kim 1, Yu-Jin Jung 2, Kwon-Kyoo Kang 2, Ku-Hyun Kwon 1, Sang-Heon Choi 1, Michael Kovach 3, Susan McCouch 3*, Yong-Gu Cho 1* A deletion analysis of the Oryza sativa dihydroflavonol reductase (DFR) promoter defined a 25 bp region (-386 to -362) sufficient to confer pericarp-specific expression of a β-glucuronidase (GUS) reporter gene in transgenic rice. Site-specific mutagenesis of these conserved sequences and subsequent expression analysis in calli which transiently expressed the mutated promoter::gus gene showed that both bhlh (-386 to -381) and Myb (-368 to -362) binding sites in the DEL3 (-440 to 70) promoter were necessary for complete expression of the GUS gene including the tissue-specific expression of DFR::GUS gene. The GUS gene was expressed well in the mutated Myb (-368 to -362) binding site, but not as strong as in normal condition, implying that the Myb is also necessary to express GUS gene fully. Also, we found the non-epistatic relation between Rc and DFR. There were no changes of expression patterns GUS under the Rc and rc genotypes. Thus, DFR expression might be independent of the presence of functional Rc gene and suggested that Rc and Rd (DFR) share the same pathway controlling the regulation of flavonoid synthesis but not a direct positive transcriptional regulator of DFR gene. These authors contributed equally to this work. This work was supported by a grant from the Next-Generation BioGreen 21 Program (PJ ), RDA, Republic of Korea. *Corresponding Author: Tel , ygcho@cbnu.ac.kr 192

249 PE-19 JinSu Lee * Brassinosteroids (BR) are plant steroid hormones playing crucial roles in diverse growth and developmental processes in plant life cycles. The canonical BR signaling and its crosstalks with other signaling pathways are involved in pleotropic regulation of plant growth and development. However, their biological roles in one of the most important crops, tomato (Solanum lycopersicum), are largely unknown. Here, we performed comparative physiological and in-silico analysis between cultivated wild-type tomato, BGA and a BR biosynthetic mutant, micro-tom (MT). As previously reported, the BR-deficient MT tomato displayed stunt growth phenotypes and we further revealed abnormal xylem development in tomato stem tissues. These BR-defective phenotypes were completely recovered by either exogenous epi-bl treatments or complementation of BR biosythetic and signaling components. Conversely, impaired BR signaling pathways in tomato by overexpressing SlGSK3s or knocking-out SlBRI1 with a CRISPR-Cas9 genome editing system were resulted in more severe defects in the xylem developments. Using RNA-seq and bioinformatic analysis of a BR defected MT tomato plant, we confirmed the correlation between BR signaling pathways and diverse development or stress related gene networks. Furthermore, we successfully selected and analyzed a putative novel direct target gene for the BR-mediated xylem development. The molecular functional studies of the novel target gene showed that BR directly modulate auxin homeostasis during the xylem formation. In this study, we reveal BR-mediated novel molecular networks for plant vascular developments and signaling integration with auxin in the important crop tomato plant. *Corresponding Author: Tel , jinsulee90@gmail.com PE-20 Jinsu Gil 1, Jaebok Lee 1, Sang Ik Park 1, So Hyeon Park 1, Kyoungmin Lee 1, Sung Cheol Koo 2, Ho Bang Kim 3, Yi Lee 2* Codonopsis lanceolata has been used for a long time in the private sector since its efficacy and safety had been proven in East Asian countries including Korea, China, and Japan. In Korea, most of them are cultivated in mountain farms and they are prescribed as therapeutic characteristics in oriental medicine. However, the cultivation remains only at the farm level, and the basic genetic studies are inferior to the element and efficacy studies. Although C. lanceolata is also a high-efficiency crop, but standard cultivar was not developed yet. Therefore it needs to establish an early screening system for genetic resources that can be used for breeding. In this study, we developed SSR (Simple Sequence Repeat) markers based EST (Expressed Sequence Tag) by analyzing the transcriptomes of C. lanceolata and hope the markers could be used for the breeding and trait-related genes discovery from C. lanceolata in the futures. Acknowledgement: This work was carried out with the support of "Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ )" Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , leeyi22@cbnu.ac.kr 193

250 PE-21 Suyeon Kim 1*, Sun Mi Huh 1, Hay Ju Han 1, Myung Hee Nam 3, Ju Hee Jung 3, Gang Seob Lee 2, Yong Sic Hwang 4, Beom Gi Kim 1 and In Sun Yoon Seed dormancy is an important agronomic trait affected by complex genetic and environmental interactions not yet comprehensively understood. By screening of rice mutants generated by a Ds transposable element, we identified a viviparous mutant dor1. Caryopses of the dor1 mutant display opaque endosperm with abnormal morphology of aleurone layers, and germinate faster at developing stages as well as in mature state. A single insertion of Ds element was found at the second exon of a novel gene Dor1. The viviparous and opaque phenotype of dor1 mutant caryopses was complemented by reintroduction of the Dor1 gene. Ectopic expression of the Dor1 gene in transgenic rice and Arabidopsis enhanced seed dormancy. Dor1 preferentially expressed in the seed embryo and aleurone cells and showed no homology with known proteins except a short glycine-rich domain. We determined that Dor1 protein is capable to bind to the GA receptor protein GID1 and other hormone sensitive lipase. The dor1 mutant featured a hypersensitive GA-response of α -amylase gene expression during seed germination. The combined results suggested that Dor1 protein is a putative negative player of GA signaling operated in the maintenance of seed dormancy. Supported by grants (PJ and PJ ) from RDA. *Corresponding Author: ggyu0116@korea.kr PE-22 Kyoungwon Cho 1*, Oksoo Han 1* 1 The accumulation of transcriptomic data from diverse stress conditions, development stages, organs and cultivars and gene co-expression network analysis can provides precise information for identifying the function of novel genes. Up to now, we have gathered 210 transcriptome datasets resulted from Agilent rice 44K DNA chip in NCBI GEO and our research team. Systemic network analysis among oxylipin-related genes including lipoxygenase (LOX), allene oxide synthase (AOS), hydroperoxide lyase (HPL), allene oxide cyclase (AOC) and 12-oxo phytodienoic acid reductase (OPR) provided candidate genes participating a novel oxylipin pathway. Indeed, the characteristic analysis of protein encoded by the candidate gene showed that the gene participate in the new oxylipin pathway (9-lipoxygenase pathway), indicating that the network analysis of gene to gene using transcriptome could be a powerful approach to mine novel genes regulating rice seed storage proteins such as prolamin, glutelin and globulin. For the network analysis, Pearson correlation analysis was performed between genes encoding prolamin, glutelin and globulin and all genes (43494) on rice DNA chip. We selected genes with high correlation coefficient value, annotated their functions, and then found genes involved in rice seed storage proteins. *Corresponding Author: KC: kw.cho253@gmail.com, OH: oshan@jnu.ac.kr 194

251 PE-23 Yuna Kang 1*, Hak Soo Seo 1 1 Sumoylation regulates numerous cellular functions including the localization, level and stability of various proteins in plants as well as animal systems. However, the regulatory mechanisms controlling E3 SUMO ligase are poorly understood. Here, sumoylation and ubiquitination of the Arabidopsis E3 SUMO ligase AtSIZ1 was specifically regulated by abiotic stresses. AtSIZ1 ubiquitination was induced by exposure to heat stress in transgenic plants overexpressing the E3 ubiquitin ligase COP1. In addition, AtSIZ1 ubiquitination was strongly enhanced in transgenic plants overexpressing SUMO isopeptidase ESD4 under heat stress. By contrast, drought stress induced sumoylation rather than ubiquitination of AtSIZ1 and sumoylated forms of AtSIZ1 accumulated in esd4and cop1-4 mutants. Moreover, siz1 mutants were found to be tolerant to heat and drought stresses. Our data indicate that ubiquitination and sumoylation of AtSIZ1 in response to abiotic stresses depend on the activities of COP1 and ESD4, and that the activity and stability of AtSIZ1 can be specifically controlled by different abiotic stresses. Acknowledgement: This work was supported by a grant from the Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center no. PJ ), Rural Development Administration, Republic of Korea. *Corresponding Author: abcd7892@naver.com PE-24 박도이 1, 양은영 2, 조명철 2, 채수영 2, 사무엘티라훈 2, 이준대 1* 1 2 당뇨병은전세계적으로심각한문제가되는질병중의하나이며그환자수가매년급격히증가하고있다. 당뇨병은인슐린의분비량이부족하거나정상적인기능이이루어지지않는대사질환의일종으로혈중포도당의농도가높아지는고혈당을특징으로하며이때심각한합병증을유발한다. 알파 - 글루코시데이즈 (a-glucosidase) 는이당류를단당류로분해하여소장에서의탄수화물흡수를촉진시키는효소이다. AGI(a-glucosidase inhibitor) 는이분해효소의활성을억제하여탄수화물의흡수속도를지연시켜체내의혈당을낮춰주는기능을한다. 현재의료용으로사용되는경구혈당강하제는미생물로부터추출한것들이며소화기장애와같은부작용을초래하기도한다. 때문에경구혈당강하제를대체할천연물질을찾고자많은선행연구들이진행되었다. 그중고추에서 AGI 함량이높다는보고가있었으며고추의과실보다잎에서더높은활성을보인다는결과가있었다. 이러한결과를바탕으로 AGI 고활성잎전용고추품종을육성하고자하며이를위해고추잎에서 AGI 활성분석방법의확립이필요하다. 기존에알려진 AGI 활성분석방법을따라, 추출용매로에탄올을사용하면잎의엽록소도같이추출되어 AGI 활성을측정하는데문제가되었다. 이러한문제를해결하기위해첨가된 a-glucosidase 및기질 (pnpg) 용량을변화시켜실험을수행하였고, 또한추출되는엽록소를줄이기위해에탄올대신에물로추출하는실험도수행하였다. 고추과실에서추출용매로물과에탄올을사용하였을때 AGI 활성정도가비슷하였지만, 고추잎에서는두추출용매를사용하였을때 AGI 활성에차이를보였다. 또한에탄올에서추출된엽록소의흡광도값을낮추기위하여마지막단계에 sodium carbonate 로희석되는양을다양하게하여분석하여보았다. 희석되는양을두배로늘린결과엽록소의흡광도값이약 1/6 로감소하였다. 개선된분석방법을사용하면에탄올에의해추출되는엽록소에크게영향을받지않고 AGI 활성을분석할수있었다. * 교신저자 : Tel , ajfall@jbnu.ac.kr 195

252 PE-25 Hayoung Moon 1*, Maxim Prokchorchik 1, Ankita Pandey 1, Gayoung Jung 1, Honour C. McCann 2, Hongsik Won 3, Kwangsoo Cho 3, and Kee Hoon Sohn 1, Ralstonia solanacearum(rso), the causal agent of bacterial wilt disease, is one of the most devastating phytopathogens. With a surprisingly broad host range, it threatens more than 200 plant species, including major crops such as potatoes, tomatoes, peppers, etc. It is so genetically heterogeneous in nature that it is difficult to develop disease resistance against it. Rso translocate special molecules (known as effectors) into their plant host cells in order to effectively suppress plant immunity. One of the most important objectives for plant breeding is to confer disease resistance. These days, effectors are used as an uprising tool to breed disease resistance, referred to as effector-assisted molecular breeding. We aim to comparatively analyze Rso genomes and investigate their effector diversity and its interactions with host plants. We sequenced 30 Rso strains, which were isolated from different host plants in different geographic regions of South Korea. We applied comparative genomics approach to investigate the phylogenetic structure of this strain set and currently we report that, surprisingly, these strains show high level of genetic diversification and significant phylogenetic distances even in a single phylotype. We also screened wild-type potato plants and their relatives against the Rso Korean strains and identified avirulence gene candidates based on the comparative genomic analysis. The gene candidate constructs would be cloned or synthesized to be tested whether they can induce plant disease resistance. We hope it contribute to the development of effector-assisted disease resistant potato breeding system so expect it to accelerate breeding for disease resistant lines. *First Author: Tel , hayoung.moon@postech.ac.kr PE-26 Chang-deok Han *, Vikranth Kumar Enhancing yield has been a major challenge of agriculture. In rice, tiller number is one of the important biomass and yield components. A maize mutant grassy tillers1(gt1) increases lateral branches in maize. The GT1gene encodes a class I homeodomain leucine zipper (HD-Zip) protein. In maize, the gt1expression is induced by shading and is dependent on the activity of teosinte branched1(tb1), a major domestication locus controlling tillering and lateral branching. To estimate the biological role and agricultural utility of gt1in rice, rice homologue (OsGT1) has been isolated and its overexpressors and RNAi lines were generated. Field data have shown that OsGT1overexpressors reduce the number of tillers and panicles while RNAi lines increase them, compared to ones of wild type. Since shade signal is an important factor in determining lateral branching, the relationship between OsGT1and shade avoidance has been explored. Plants have been grown under 50% shading in the field. Also, double genetic combinations with phytochrome mutants (phya, B, and C) are being examining for tillering phenotype. These researches could provide insights in determining the action of OsGT1on branching and shade avoidance in rice. This research is supported by the Plant Molecular Breeding Center of PostBiogreen 21 *Corresponding Author: Tel , cdhan@gnu.ac.kr 196

253 PE-27 Su Jang, Choonseok Lee, Jin seok Moon, Mikyong Kang, Hee-Jong Koh * Balancing panicle-related traits such as panicle length and the numbers of primary and secondary branches per panicle, is key to improving the number of spikelets per panicle in rice. Identifying genetic information contributes to a broader understanding of the roles of gene and provides candidate alleles for use as DNA markers. Discovering relations between panicle-related traits and sequence variants allows opportunity for molecular application in rice breeding to improve the number of spikelets per panicle. In total, 142 polymorphic sites, which constructed 58 haplotypes, were detected in coding regions of ten panicle development gene and 35 sequence variants in six genes were significantly associated with panicle-related traits. Rice cultivars were clustered according to their sequence variant profiles. One of the four resultant clusters, which contained only indica and tong-il varieties, exhibited the largest average number of favorable alleles and highest average number of spikelets per panicle, suggesting that the favorable allele combination found in this cluster was beneficial in increasing the number of spikelets per panicle. Favorable alleles identified in this study can be used to develop functional markers for rice breeding programs. Furthermore, stacking several favorable alleles has the potential to substantially improve the number of spikelets per panicle in rice *Corresponding Author: Tel , heejkoh@snu.ac.kr PE-28 You-Ran Jang, Joo Ohk Sohn, Su-Jin Choi, Sun-Hyung Lim, Jong-Yeol Lee * High-molecular weight glutenin subunits (HMW-GSs) play an important role in bread making quality. In this study, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was established to identify HMW-GSs. Four major factors influencing mass spectra including solvent components, resolving volume, matrix II components and treatment with alkylation reagent in glutenin extraction were optimized using HMW-GS of Chinese Spring. To obtain accurate molecular weights for individual HMW-GSs, 24 standard wheat cultivars covering all HMW-GSs in hexaploid wheat were analyzed 3 times using this optimized MALDI-TOF-MS method. 38 Korean wheat cultivars previously determined using RP-HPLC and SDS-PAGE were used to verify the allelic compositions. 675 wheat crossing blocks that were harvested by RDA National Institute of Crop Sciences were also used to analyze the composition of HMW-GSs. Results showed that some varieties have 1Ax2*, 1Bx7 OE, 1Bx17+1By18, 1Dx5+1Dy10 which are specifically associated with good breed making quality.although 3-5 subunits are usually expressed in common bread wheat cultivars, but two lines have only two subunits. HMW-GSs of a wheat cultivar are analyzed within one minute by MALDI-TOF-MS, so it is expected to be suitable for the high-throughput analysis of HMW-GSs. MALDI-TOF-MS will be useful to improve end-use quality in wheat breeding programs. *Corresponding Author: Tel , jy0820@korea.kr 197

254 PE-29 Nam-Chon Paek *, Kiyoon Kang In rice, moderate leaf rolling increases photosynthetic competence and raises grain yield; therefore, this important agronomic trait has attracted much attention from plant biologists and breeders. However, the relevant molecular mechanism remains unclear. Here, we isolated and characterized Rolled Fine Striped (RFS), a key gene affecting rice leaf rolling, chloroplast development, and reactive oxygen species (ROS) scavenging. The rfs-1 gamma-ray allele and the rfs-2 T-DNA insertion allele of RFS failed to complement each other and their mutants had similar phenotypes, producing extremely incurved leaves due to defective development of vascular cells on the adaxial side. Map-based cloning showed that the rfs-1 mutant harbors a 9-bp deletion in a gene encoding a predicted CHD3/Mi-2 chromatin remodeling factor belonging to the SNF2-ATP-dependent chromatin remodeling family. RFS was expressed in various tissues and accumulated mainly in the vascular cells throughout leaf development. Furthermore, RFS deficiency resulted in a cell death phenotype that was caused by ROS accumulation in developing leaves. We found that expression of five ROS-scavenging genes (encoding catalase C, ascorbate peroxidase 8, a putative copper/zinc superoxide dismutase, a putative superoxide dismutase, and peroxiredoxin IIE2) decreased in rfs-2 mutants. Western-blot and chromatin immunoprecipitation (ChIP) assays demonstrated that rfs-2 mutants have reduced H3K4me3 levels of ROS-related genes. Loss-of-function in RFS also led to multiple developmental defects, including pollen development, grain filling, and root development. Our results suggest that RFS is required for many aspects of plant development and its function is closely associated with epigenetic regulation of genes that modulate ROS homeostasis. *Corresponding Author: Tel , ncpaek@snu.ac.kr PE-30 Jina Bae and Ilha Lee * The suppression of floral repressor, FLOWERING LOCUS C (FLC), is a crucial mechanism for the promotion of flowering in Arabidopsis. VERNALIZATION INSENSITIVE 3 (VIN3) encoding a PHD finger domain protein is associated with the epigenetic repression of FLC. The induction of VIN3 is mediated by long-term winter cold, called vernalization, but how VIN3 is transcriptionally regulated is poorly understood. Here we show that EMF1 recruits a HISTONE DEACETYLASE 19 (HDA19) and directly suppresses VIN3 by histone deacetylation. To find upstream regulator of VIN3, we performed mutagenesis and isolated a mutant which exhibiting reduced activation of VIN3. A VIN3 hyposensitive mutant, called rev79 (reduced activation of VIN3), contains a T-DNA at the promoter region of EMBRYONIC FLOWER 1 (EMF1), and EMF1 is overexpressed in rev79. So we named this mutant emf1-101d. emf1-101d had a similar VIN3 induction pattern during the vernalization, but the level of transcription was reduced compared to WT. Consistently loss-of-function mutant, emf1-1 and emf1-2, showed higher expression of VIN3 at both with and without vernalization. We further demonstrate that EMF1 forms a protein complex with HDA19 and hda19 mutant showed higher expression of VIN3. To better understand how EMF1 regulates target gene, VIN3, we examined the dynamics of histone modification patterns during vernalization and found that histone H3 and H4 acetylation were reduced at VIN3 locus in emf1-101d. Thus, our data suggest that EMF1 controls vernalization sensitivity through histone deacetylation of VIN3. *Corresponding Author: ilhalee@snu.ac.kr 198

255 PE-31 Yoon-Sun Hur *, Sunghan Kim and Choong-Ill Cheon Plants absorb photosynthetically active radiation (PAR, nm) to generate carbohydrates for the energy source. The chlorophylls of leaves selectively absorb the red (R) and blue light, whereas they reflect and transmit the far-red (FR) light. Total light intensity or R:FR ratio are reduced in the dense vegetation. To obtain the unfiltered light, plants in the shade promote the hyponastic growth, hypocotyl elongation and petiole elongation, a response known as the shade avoidance syndrome (SAS). As shade promotes the immature and rapid elongation of leaf-bearing organs and early flowering, SAS affects the vegetable crop yield significantly. In shade-avoiding plants such as Arabidopsis, this changes in light quality and quantity are sensed by phytochrome (phy) photoreceptors. Under normal vegetation, active phytochrome (Pfr) interact with phytochrome-interacting factors (PIFs). We have sought ways to regulate SAS for modulating the plant response, so that crop yield can be maintained with compromised responses to shade condition. Expression of some transcription factors, known and unknown, have been regulated by transgenics overexpressing them or expressing amirnas against them. Transgenic plants under simulated shade conditions have been analyzed. These approach should give us insights on how we can modulate crop plants for preventing significant yield loss due to SAS. *Corresponding Author: Tel , yshur@sookmyung.ac.kr PE-32 Ho Jun Joh, Hong-Seob Yu, Hyeonah Shim, Hyun-Seung Park, Sae Hyun Lee, Shin-Jae Kang, Tae-Jin Yang * Peucedanum japonicum, a perennial herbal plant species of Apiaceae family, was initially cultivated as oriental medicine is nowadays being cultivated as healthy edible vegetable. Previous study, we have discovered two types (Long/Short) of inverted repeats (IRs) correlated with the sequence polymorphisms in other regions of chloroplast (cp) genomes through comparative analysis of collected wild accessions. To extend further to this study, we have applied the InDel marker to the collected wild and cultivate P. japonicum accessions and learned that the band pattern in the long type P. japonicum has lesser variation than the band pattern from the short type P. japonicum. For faster and efficient method to differentiate the IR type of each P. japonicum accession, we have designed the SNP dcaps marker in advance to the KASP marker for later experiment. To specifying more intra-species variations as well as genotyping wild accessions of P. japonicum, 45S nuclear ribosomal DNA sequences, and simple sequence repeats (SSRs) was examined. Comparative analysis on 45S nuclear ribosomal DNA sequences, resulted three SNP polymorphism has been found, including two types of SNPs located within 26S region. SSR markers was able to detect two type of allele among seven P. japonicum accession. Taken together, sequences and polymorphisms identified in this study will be valuable genetic resources that can be applied for molecular breeding of P. japonicum. This research was supported by Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ013238), Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , tjyang@snu.ac.kr 199

256 PE-33 강유나 1*, Jong Tae Song 2, Si Wan Ryu 3, Hak Soo Seo Doubled haploids (DHs) in maize breeding programs reduce the time required to generate and evaluate new lines to 2 years or less. However, efficient techniques that accurately discriminate between haploid and diploid maize kernels are required. Here, we investigate the effects of several hormones and chemicals on the germination of haploid and diploid maize kernels, including auxin, cytokinin, ethylene, abscisic acid (ABA) biosynthesis inhibitor (fluridone), ABA catabolism inhibitor (diniconazole), methyl jasmonate (MeJA), and NaCl. Ethylene effectively stimulated the germination of both haploid and diploid maize kernels. The ABA biosynthesis inhibitor fluridone, the ABA catabolism inhibitor diniconazole, and MeJA selectively stimulated the germination of haploid maize kernels. By contrast, gibberellin, 1-naphthaleneacetic acid (NAA), kinetin, and NaCl inhibited the germination of both haploid and diploid maize kernels. These results indicate that the germination of haploid maize kernels is selectively stimulated by fluridone and diniconazole, and suggest that ABA-mediated germination of haploid maize kernels differs from that of diploid maize kernels and other plant seeds. This work was supported by a grant from the Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center no. PJ ), Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , abcd7892@naver.com PE-34 김영천, 한지훈, 이상협 * 고추의매운맛은캡사이시노이드 (capsaicinoids) 물질에의해나타나며, 그중캡사이신 (capsaicin) 과다이하이드로캡사이신 (dihydrocapsaicin) 이양적인측면에서주를이루고있다. 따라서캡사이시노이드를합성하는 Pun1 (acyltransferase) 유전자의기능이소실되면매운맛이없어진다고알려져있다. 매운맛에대한최근까지의연구는 Pun1 유전자에집중되어진행되어왔지만, 고추유전체서열분석결과에의하면최소 3 개이상의 Pun1 homologs 가존재하는것으로알려져있다. 따라서본연구에서는이들유전자들과매운맛과의관계를규명하고자한다. Acknowledgement: This work was supported by Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center No. PJ ) of Rural Development Administration of Korea. * 교신저자 : Tel , sanglee@sejong.ac.kr 200

257 PE-35 김영천, 이주영, 이상협 * Alzheimer s disease (AD) involves accumulation, oxidative damage and inflammation and there is currently no clinically accepted treatment to stop its progression. Its risk is known to reduce with increased consumption of antioxidant and anti-inflammatory agents. Fibrillar aggregates of A are major constituents of the senile plaques found in the brains of AD patients and have been related to AD neurotoxicity. The senile plaques are produced by the sequential cleavage of the amyloid precursor protein by secretases. Recently peppers are noted for their antiaging and cognitive enhancing properties. Thus, in this study, the effects of total extracts from pepper on amyloid production and aggregation in vitro were investigated. Our study indicate that pepper could be a possible dietary intervention into the management of AD. Acknowledgement: This work was supported by Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center No. PJ ) of Rural Development Administration of Korea. * 교신저자 : Tel , sanglee@sejong.ac.kr PE-36 김영천, 최다은, 이상협 * Abstract. Plant-based food have been used to treat diabetes mellitus recently, becausethe number of type II diabetic patient has increased dramatically in worldwide. The effective diabetes prevention is the controlling of postprandial hyperglycemia by α -glucosidase inhibition. This study present data of α -glucosidase inhibitory activity in extracts of 14 pepper fruits (Capsicum annuum L.) highly consumed as vegetable in Korea. The pepper fruit extracts were prepared with 70% ethanol and water, subjected to dilution (50 and 25%) prior to α-glucosidase inhibitory assay. Significant differences of α -glucosidase inhibitory activity were not shown between two different aqueous extract. Mee-In and Dda-Go-Ddo-Dda-Go cultivars possessed highest and sustainable inhibitory activity (almost 100%) against α -glucosidase. Whereas, the extracts from Soon-Han-Gil-Sang, Gil-Sang, Sweety, Hyul-Jo K, Shin-Hong, and Ai-Mat showed sharp decreasing pattern between 100 and 50% extract concentration. These results suggest the possibility that existence of unknown inhibitors at different concentration in the different pepper cultivars. Hence, further studies may needed to identify the unknown key α -glucosidase inhibitors in pepper fruits. Acknowledgement: This work was supported by Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center No. PJ ) of Rural Development Administration of Korea. * 교신저자 : Tel , sanglee@sejong.ac.kr 201

258 PE-37 Jinseul Kyung, Myeongjune Jeon * and Ilha Lee Vernalization is a process that plants acquire competence to flower after long-term winter cold. How plants recognize long-term cold is still in veil although the molecular mechanism of winter cold memory has been studied well. In Arabidopsis, VERNALIZATION INSENSITIVE 3 (VIN3), which confers long-term cold memory by mediating epigenetic silencing of floral repressor(s), is the only known protein-coding gene induced by vernalization. To understand the mechanism how plants sense vernalization, we identified cis-element responding to long-term cold from VIN3 promoter. This cis-element is named as VRC (vernalization responding cis-element). Plants became vernalization-insensitive when the motif is mutated. We also found CCA1 and LHY, evening element-binding circadian rhythm regulators, are involved in VIN3 induction during vernalization process. They directly bound to VRC and vernalization-mediated VIN3 induction was impaired in cca1 lhy mutant. The prolonged cold dampens the diurnal rhythms of CCA1 and LHY, thus their level at certain time, i.e. at dusk, gradually increases during vernalization. The altered rhythm of CCA1 and LHY is coincident with VIN3 expression pattern, which shows peak at dusk. Taken together, VIN3 transcription is induced by rhythmic change of circadian rhythm regulators, CCA1 and LHY, during vernalization. This study would be adopted for agricultural development. Chinese cabbage (Brassica rapa pekinensis) has conserved VIN3 homologs. We found that among the three VIN3 homologs in Chinese cabbage, two of them have the conserved VRC. This result implies that Chinese cabbage has conserved vernalization-response mechanism. Therefore, it is plausible to generate vernalization-insensitive, thus more productive, Chinese cabbage by editing VIN3 homologs. *Corresponding Author: Tel , iammj2@snu.ac.kr PE-38 Hyo-Ju Lee 1, Ye Rim Lee 1, Hee Kyung Kim 1, Yeo Jin Jung 1, Sangsu Bae 3, Ji Hyeon Yu 3, Kwon Kyoo Kang 1,2, Yu Jin Jung 1,2* Genome editing technology have been successfully applied to improve various crop traits such as ZFN, TALEN and CRISPR/Cas9 system. Among them, CRISPR/Cas9 system is now conveniently available as a precise and efficient genome engineering tool. Targeted mutagenesis using CRISPR/Cas9 system is especially useful for plant breeding and gene functional analysis. In this study, we applied the CRISPR/Cas9 system to modify rice seed colors. Binary vectors harboring expression cassettes of Cas9 nuclease, single guide RNA (sgrna) targeting the leucoanthocyanidin dioxygenase (LDOX) and bar as a selectable marker gene were constructed and developed transgenic rice plants via Agrobacterium-mediated methods. Targeted mutations were analyzed in bar-resistant shoots by NGS analysis. Transgenic plants clearly contained DNA mutations such as nucleotide substitutions, insertions and deletions at the target site, which varied depending on the transgenic lines. Also, these mutant lines showed that seed color modified. Therefore, these data will be discussing relationship between modification of seed-color (phenotypic variation) and mutagenesis of target site (genotypic variation) in transgenic plants. *Corresponding Author: Tel , yuyu1216@hknu.ac.kr 202

259 PE-39 Nuri Oh 2, Yong-Jin Shin 1, Jimin Lee 1, Jaeho Kim 1, Pil Joong Chung 2 and Choonkyun Jung 1,2* 1 2 Recently, long noncoding RNAs (lncrnas) have emerged as important regulatory factors of diverse biological processes in both plants and animals. However, the number and functional roles of lncrnas in crops remain largely unknown. In particular, systematic examination of rice lncrnas involved in abiotic stress responses has not been performed. In this study, we re-analyzed the expression profile of lncrnas in publicly available rice transcriptome datasets derived from abiotic stress treatments to unveil the potential roles of rice lncrnas in abiotic stress responses. Overall, we identified 10,831 rice lncrnas that were significantly altered in shoot and/or root tissues under four different abiotic stresses, including ABA, drought, cold, and high salt. Out of them, 5,516 and 5,315 rice lncrnas were upregulated or downregulated, respectively, in a highly stress-specific or tissue-specific manner. These stress-responsive lncrnas were classified into 15 groups from their stress-specific expression patterns. Based on Venn diagram analysis, we observed strong crosstalks between different stress signaling pathways, showing transcriptional regulatory networks underlying lncrna expression changes in response to abiotic stresses. Lastly, qrt-pcr validation confirmed the differential expression patterns of these lncrnas under various conditions. This study shows the first comprehensive identification of a group of rice lncrnas that are involved in abiotic stress responses. The results suggest that rice lncrnas may play crucial roles in abiotic stress tolerance mechanisms. *Corresponding Author: Tel , jasmin@snu.ac.kr PE-40 Bo-ram Choi 1, Soo-Jung Han 2, Siwon Choi 2, Kyeong-Jae Heo 1, Myung-Hwi Kim 1, and Jang-Kyun Seo 1,2 1 2 While pepper (Capsicum annuum L.) is a highly recalcitrant species with respect to current genetic transformation technologies, plant virus-based vectors can provide alternative and valuable tools for transient regulation and functional analysis of genes of interest in pepper. In this study, we established an effective virus-based vector system applicable for over-expression of genes of interest in pepper using broad bean wilt virus 2 (BBWV2). We engineered a single gene insertion cassette between the movement protein and large coat protein-encoding cistrons in RNA2 by modifying the infectious cdna clone of the BBWV2 strainrp1 which cause no visible symptoms in pepper) and named in as pbbwv2-oe vector. Successful expression of GFP from the BBWV2 vector was observed in various pepper cultivars and other plant species including Nicotiana benthamiana,arabidopsis thaliana, and Perilla frutescens var. japonica Hara during the early infection stage of the virus. However, the GFP expression level was gradually decreased as viral infection progressed systemically in pepper. To overcome this phenomenon and enhance GFP expression, we sought to examine co-expression of a viral RNA silencing suppressor together with GFP from the BBWV2 vector. To this end, we first engineered an additional gene insertion cassette into pbbwv2-oe and this dual-gene expression vector was named as pbbwv2-oex2. Next, to investigate the effects of viral RNA silencing suppressors on enhancing gene expression from the BBWV2-based vector, GFP was simultaneously expressed with viral RNA silencing suppressors including the tomato bushy stunt virus p19, cucumber mosaic virus 2b, and flock house virus B2 using pbbwv2-oex2 in N. benthamiana and pepper.the results showed that GFP expression was most effectively enhanced by B2 when co-expressed from the BBWV2-based vector, while 2b has only marginal effect on enhancing GFP expression and co-expression of p19 resulted in increasing symptom severity. This BBWV2-based gene delivery system provides a convenient approach for rapid and simple gain-of-function studies in pepper. *Corresponding Author: Tel , jangseo@snu.ac.kr 203

260 PE-41 In-Taek Hwang, Na-Rae Jeong, Heejin Kim, Jin-Ho Kang * Potato is one of the important crops in the world. Cultivated potatoes are vulnerable to insect attacks. However, several wild potato species including S. berthaultii are resistant to insect herbivories. To find genes related to insect resistance, we performed a comparative transcriptome analysis between cultivated potato S. tuberosum and wild potato S. berthaultii. RNA-seq data showed 679 DEGs were up-regulated and 303 DEGs were down-regulated in S. berthaultii compared to S. tuberosum. Among them, 145 genes were related to biosynthesis of terpenoids and flavonoids, which are well known for insect resistant metabolites. The expression level of genes involved in terpenoids and flavonoids biosynthesis pathways was higher in S. berthaultii than S. tuberosum, and these results were confirmed by qrt-pcr. GC-MS analysis confirmed that the higher expression of those transcripts in S. berthaultii led to the higher accumulation of several terpenoids. These results suggest that secondary metabolites including terpenoids play an important role in plant defense against insect attack. *Corresponding Author: Tel , kangjinho@sun.ac.kr PE-42 Kyu Jin Sa 1, Ik-Young Choi 2, Kyong-Cheul Park 2, Ju Kyong Lee 1* 1 2 We used Illumina/HiSeq sequencing to analyses gene expression profiling among the four maize seed at 10 DAP (days after pollination). we generated total 88,993,000 (CM3), 103,817,340 (CM6), 103,139,640 (CM5), 66,978,958 (CM19) sequence reads with read length of about 0.9, 1.0, 1.0 and 0.7 billion bp. We obtained 69.1 (CM3), 71.0 (CM6), 71.2 (CM5), 71.8 (CM19) % high quality reads from the raw data. we compare to the reference RNA sequence in the public DB (NCBI). We revealed that mapped reads were 58%, 63%, 62% and 62% of the EST reference in CM3, CM6, CM5 and CM19. more than 51,000 genes were expressed based on RPKM criteria (over 0.25 value) in each CM3, CM6, CM5 and CM19 inbred lines. In DEG analysis, we found that 3,527 genes were differentially expressed with at least two times with 1,709 up-regulated in waxy maize inbred lines and 1,818 up-regulated in dent maize inbred lines. To identify expressions of DEGs associated with metabolic pathways using MAPMAN, some genes upregulated in waxy and dent were only expressed in independent metabolic pathway for dent upregulated genes, such as cell wall proteins.agps, degradation.mannan-xylose-arabinose-fucose (in cell wall), nitrate metabolism, N-degradation (in N-metabolism), synthesis.glutamate family.proline, synthesis.aromatic aa.chorismate, synthesis.histidine, degradation.serine-glycine-cysteine group.glycine (in amino acid metabolism), simple phenols, N misc (in secondary metalbolism), ascorbate and glutathione (in reduction-oxidation), synthesis.pyrimidine, phosphotransfer and pyrophosphatases, deoxynucleotide metabolism (in nucleotide metabolism), non-reductive PP (in OPP), and for waxy upregulated genes such as pectin*esterases (in cell wall), synthesis for aspartate family.lysine, branched chain group.common, branched chain group.leucine specific, serine-glycine-cysteine group.glycine, serine-glycine-cysteine group.cysteine, aromatic aa.phenylalanine and tyrosine and degredation for glutamate family.glutamine, serine-glycine-cysteine group.cysteine, aromatic aa.tryptophan (in amino acid metabolism), trehalose.tpp (in minor CHO metabolism), S-assimilation. The differently expressed gene (DEGs) profiling set and pathway analysis can server to understand of gene regulation depends on kernel types in dent and waxy maize. *Corresponding Author: Tel , jukyonglee@kangwon.ac.kr 204

261 PE-43 Ju Seok Lee 1, Kyung Hae-Kim 2, Ji-Min Kim 2, Juhyun Im 1, and Sungtaeg Kang 2* 1 2 The sap-sucking insects infect a wide range of host plants worldwide. The foxglove aphid, Aulacorthum solani (Kaltenbach), is a Hemipteran insect that causes serious yield losses in soybean. The objective of this study was to narrow down the Raso2 region, which is previously identified QTL conferring foxglove aphid resistance, using high-density SNP array, Axiom 180K SoyaSNP, and identify the candidate genes. The F 4:8 recombinant inbred lines were developed from a cross between susceptible Williams82 and resistant wild soybean PI , were used to QTL analysis. The antibiosis and antixenosis were evaluated through choice and no-choice assays with total plant damage and primary infestation leaf damage. The high-density genetic linkage map was constructed with total 169,028 SNPs. Using inclusive composite interval mapping analysis, Raso2 on chromosome 7 narrow down to 3cM, corresponding to 76-kb of genomic region based on the Williams 82 genome assembly (Wm82.a2.v1) including 8 annotated gene models. Furthermore, comparing the nucleotide sequences of eight gene models between both parents, Williams 82 and PI , total 11 SNPs with 4 nonsynonymous substitution were identified. Interestingly, all 4 nonsynonymous substitution were located in Glyma.07g077700, which contain NB-ARC domain. In conclusion, we narrow down the Raso2 up to 76-kb interval, and identified the 4 nonsynonymous SNPs on Glyma.07g using genome-wide high-density SNP assay. These result could provide the useful information in breeding for new foxglove aphid resistant soybean cultivar. *Corresponding Author: Tel , kangst@dankook.ac.kr PE-44 Young-Sang Lee 1*, Kyu-Won Kim 2, Yoo-Hyun Cho 3, Yong-Jin Park Paradigm of Korean rice breeding is recently shifting from yield to palatability and health-beneficial effects. In an attempt to develop a new rice variety superior in both fragrance and nutritional value, 19 candidate advanced breeding lines with aroma properties were selected, and their grain phytochemicals such as tocols, squalene, and phytosterols were determined, and their grain volatile profiles were also characterized by using a headspace solid phase microextraction (HS-SPME) combined with an GC/MS. From all fragrant rice breeding lines total 133 volatiles were identified, among which 46 volatiles were found commonly in all tested 19 lines, while 14 volatiles were observed in less than 5 lines. The most frequently and abundantly observed volatile was nonanal, which consisted 16.9 to 34.8% of all identified volatiles in all tested lines. A popcorn-flavoring 2-acetyl-1-pyrroline, the most determinant flavoring compound in rice exhibited 0.3 to 5.6% of volatile compositions in 18 lines. Total tocopheorols, tocotrienols, squalene, and tota phytosterols contents ranged 5.3 to 33.7, 12.3 to 19.7, 8.1 to 220 ppm, and to ppm, respectively. Based upon all results describe above, a breeding line JS-29 which showed 3.6% of 2AP composition, 8.8 ppm of total tocopherols, 12.3 ppm of total tocotrienols, ppm of total phytosterols, and 15.6 ppm of squalene contents could be selected as the most promising candidate line for a fragrant rice variety with high nutritional value. *Corresponding Author: Tel , mariolee@sch.ac.kr 205

262 PE-45 Jiyeon Jeong 1, Jin-Kyu Woo 1, Young Chul Park 2, Sukman Park 3, Su-Hyun Yun 3, Yi Lee 4, Gun-Hyoung Cho 1, Kwan Jeong Song 5, Ho Bang Kim 1* Regardless of the importance of a viral pathogen, citrus tristeza virus (CTV), in citrus industry, molecular marker tools closely associated with its resistance trait have not been developed yet. A Ctv locus, one of at least 2 CTV-resistant loci that was cloned from trifoliate orange and displayed a distinct feature of enriched R gene composition. Based on such molecular characteristics of the genetic locus, we developed multiplex PCR marker sets to specifically detect 7 R genes consisting of Ctv locus. By using the multiplex PCR marker sets, we surveyed composition of 7 R genes consisting of the genetic locus in a total of 156 citrus genetic resources including 12 of Korean citrus landraces. Genetic composition of 7 R genes in the locus was variable among the genetic resources. However, all of 7 R genes were detected only in both trifoliate orange, Poncirus trifoliata and its derivative cultivar, P. trifoliata Flying dragon, which have been reported to be resistant to CTV. Multiplex PCR marker sets established in this study would be an effective molecular tool to develop scion or rootstock cultivars with high resistance trait against CTV in citrus breeding program. Acknowledgement: This research was carried out with the support of the Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ ), Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , hobang@ibiomedic.co.kr PE-46 Hyeonso Ji 1* Jun Oh 1, Hyun-Ju Kang 1, Hyo Ja Oh 1, Song Lim Kim 1, Inchan Choi 1, Jeongho Baek 1, In Sun Yoon 1, Yong-Jae Won 2, Kyung-Hwan Kim Plentiful variations in the important agronomic traits such as disease resistance and pre-harvest sprouting exist within Korean japonica rice varieties. Therefore, it is possible to do mapping useful genes with populations derived from crosses between them. But, deficiency of molecular markers discriminating Korean japonica rice varieties has made these efforts difficult. However, abundant SNPs can be revealed between closely related crop varieties by genome sequencing enabling development of sufficient DNA markers. We collected genome sequencing data of 13 Korean japonica rice varieties to detect SNPs among them. The data size varied from 5.8 to 36 Gbp with sequencing depth of x. Using CLC Assembly Cell program and Python programs developed in-house, the genome sequence data was processed and SNPs among the varieties were identified. Totally, about 740,000 SNPs were detected. Chromosoe 11 showed the highest number of SNPs (163,557) and chromosome 5 showed the lowest number of SNPs (16,017). Currently, we are developing SNP markers to be utilized for gene mapping and maker assisted selection (MAS). *Corresponding Author: Tel , jhs77@korea.kr 206

263 PE-47 Bo-Ra Park, Da-Hye Kim, Sangkyu Park, Jong-Yeol Lee, Sun-Hyung Lim * Chrysanthemums (Chrysanthemum morifolium) exhibit a broad range of flower colors through the accumulation of anthocyanins. In this study, the cdna sequences of the seven structural genes CmCHS, CmCHI, CmF3H, CmF3 H, CmDFR, CmANS, and CmGT and two regulatory genes CmMYB, Cmbasic-helix-loop-helix (CmbHLH) involved in anthocyanin biosynthesis were isolated from three different Chrysanthemum cultivars, OB, DP, and RM. Sequence analysis revealed that most of the cdna sequences have a few variations, but bhlh cdnas from DP and RM were exactly same as the previously deposited sequence from another cultivar. Gene expression analysis showed that all of the structural genes were highly expressed in the pink flowering (DP) and red flowering (RM) cultivar compared to the white flowering (OB) cultivar. In particular, the transcript levels of CmF3H, CmF3 H, CmDFR, CmANS, and CmGT were most prominent in RM exhibiting the darkest red flower color. To demonstrate the regulatory mechanism of anthocyanin biosynthesis in the florets of Chrysanthemums, yeast two-hybrid was conducted with recombinant MYB, bhlh, and WD40 (TTG1) proteins from OB and RM, which showed that the three regulatory proteins interacted with each other, and this suggests that the anthocyanin biosynthetic pathway could be transcriptionally controlled via MBW complex. *Corresponding Author: Tel , limsh2@rda.go.kr PE-48 Taeyoung Lee 1, Suk-Ha Lee 1,2* 1 2 Seed size is one of the factors determining soybean yield. Large seed size is, therefore, preferable trait for most soybean breeders. However, small seed size is also preferred for soybean sprout in Korea. In this research, we studied genetic structure and genetic variances associated with soybean 100 seeds weight using Korean soybean landraces and cultivars. We developed 25,914 SNPs using genotype-by-sequencing and identified that Korean landraces and cultivars were clustered into 3 subgroups. Principle component analysis exhibited that landraces and cultivars were clearly divided into two groups and, cultivars were clustered depending on their usage. Genome wide association study (GWAS) showed that 30 SNPs were significantly associated with 100 seeds weight and were co-localized with 39 QTLs related to seed size. The SNPs were associated with soybean homologs to Arabidopsis seed size genes and seven of them have not been reported in previous soybean GWAS studies. We identified novel genetic factors associated with 100 seeds weight in soybean. This study will contribute to breed soybeans with preferable seed sizes for diverse usage. *Corresponding Author: Suk-Ha Lee, Tel , sukhalee@snu.ac.kr 207

264 PE-49 Jae-Hyuk Han 1, Na-Hyun Shin 1, Je-Hun Moon 2, Soo-Cheul Yoo 2, Joong Hyoun Chin 1* 1 2 Drought stress in early growing season became serious recent years in Korea. Improvement root vigor and P uptake might be a solution to overcome the problems in that stage. Early establishment by help of those traits will provide the benefits increasing yield of MS11, which is a japonica rice variety adaptive to tropical regions. Two QTLs conferring stress tolerance, DTY2.2 and Pup1, were introgressed into MS11 using marker-assisted backcrossing. Backgroud genotyping of pyramiding lines of BC 2F 5 showed that more than 95% of similarity of them to MS11. The finally selected lines were tested and they showed same in normal growth condition in the overall plant type and yield capacity. Drought stress were applied by growing in rainfed condition and MS11-drought tolerant lines showed better yielding. These lines are being grown in normal irrigation, rainfed, terminal drought, high temperature, and salinity conditions by collaborations. The pyramiding of Pup1 and drought QTLs might provide the meaningful breeding materials for the climate-ready rice development. Further, MS11-QTL lines might be considered for new candidate varieties in problem regions of south-east Asian countries. *Corresponding Author: Tel , jhcin@sejong.ac.kr PE-50 Sang-Kyu Lee, Seok-Hyun Choi, Suhyeon Shim, and Jong-Seong Jeon To elucidate the starch synthetic pathway and the role of this reserve in rice pollen, we characterized mutations in the plastidic phosphoglucomutase, OspPGM, and the plastidic large subunit of ADP-glucose (ADP-Glc) pyrophosphorylase, OsAGPL4. Both genes were upregulated in maturing pollen, a stage when starch begins to accumulate. Progeny analysis of self-pollinated heterozygous lines carrying the OspPGM mutant alleles, osppgm-1 and osppgm-2, or the OsAGPL4 mutant allele, osagpl4-1,as well as reciprocal crosses between wild type (WT) and heterozygotes revealed that loss of OspPGM or OsAGPL4 caused male sterility, with the former condition rescued by introduction of the WT OspPGM gene. While iodine staining and transmission electron microscopy analyses of pollen grains from homozygous osppgm-1 lines produced by anther culture confirmed the starch null phenotype, pollen from homozygous osagpl4 mutant lines, osagpl4-2 and osagpl4-3, generated by CRISPR/Cas system, accumulated small amounts of starch, which were sufficient to produce viable seed. Such osagpl4 mutant pollen, however, was unable to successfully compete against WT pollen, validating the important role of this reserve in fertilization. Our results demonstrate that starch is polymerized mainly from ADP-Glc synthesized from plastidic hexose phosphates in rice pollen, and that starch is an essential requirement for successful fertilization in rice. *Corresponding Author: jjeon@khu.ac.kr 208

265 PE-51 Yoo-Jung Yoon, Jelli Venkatesh, Jin-Kyung Kwon, Byoung-Cheorl Kang * Mutant lines with desirable agronomic traits have important implications for the plant breeding. The advent of CRISPR/Cas9 technology has made genome editing possible in virtually all plant species, including those plant species less amenable to genetic manipulations. However, targeted genome-editing using CRISPR/Cas9 approach in many crop plants is still in progress and optimal strategies need to be developed. In the present study, Agrobacterium mediated stable expression and TRV mediated transient expression systems are being standardized for CRISPR/Cas9 genome editing. As a proof of principle, genome editing in tomato PDS gene, a key gene functions in carotenoid biosynthesis pathway is being performed. Additionally, targeted genome editing for loss-of-function in the eif4e and eif4e-iso genes, which are known to be involved in recessive resistance of potyvirus is underway. For rapid validation of grnas, we performed in vivo Cas9 activity analyses and CRISPR-Cas9 efficiency was recorded up to 15%. Agrobacterium transformation efficiency of about % was recorded for CRISPR/Cas9-sgRNA-SlPDS, -SleIF4E and -SleIF4E-Iso constructs. Cas9 overexpressing tomato lines have been developed for TRV mediated CRISPR/Cas9 genome editing. This ongoing optimization of stable and transient expression of CRISPR/Cas9 systems has the potential for the development of an efficient procedure for targeted genome editing, and creates a platform to efficiently generate transgene-free tomato and it may provide insights into sophisticated site-specific genome engineering techniques for other crop species. *Corresponding Author: Tel. 02) , bk54@snu.ac.kr PE-52 Min-Sung Kang 1, Jung Heo 1, Sukgui Oh 2, Jong Hyang Bae 2, Soon Ju Park Regulation of shoot growth and termination is a major factor for yield improvement in tomato. self pruning (sp classic) mutant inducing shoot termination has been used for breeding field tomatoes for over 90 years. Most recent question addressed that shoot life variation under determinate growth give a new window for manipulating tomato yield in the field. In this study, we isolated new three sp mutant alleles from 242 Core Collection (C.C) lines, which show variations in shoot determinacy. One deleterious sp mutants, sp-2798, resulted in no expression of sp represented similar shoot termination with less yield than sp classic. A newsingle amino acid substitution mutant, sp-5732, produced more sympodial shoots on main shoot and axillary shoots and improved tomato fruit yield up to 42%. Therefore, we suggest that newly discovered sp alleles are new resources for manipulating shoot growth and yield of determinate tomato in the field. *Corresponding Author: Tel , sjpark75@wku.ac.kr 209

266 PE-53 Ju Hee Kim and Cheol Seong Jang * Environmental stresses such as cold, drought, high temperature, and soil salinity, negatively affect seed germination, vegetative growth, and reproductive development of plants. Soil salinity is the major agricultural problem because cause cellular dehydration and ionic toxicity and leads to a decrease in the productivity of crops. Here, we report on salt-induced RING finger E3 ligase, Oryza sativa microtubule-associated RING finger protein 2 (OsMAR2). Transcript analysis of OsMAR2 gene highly expressed at various abiotic and hormone stresses, such as ABA (100 mm), NaCl (200 mm), drought, and heat (45 C). In addition, in vitro ubiquitination assays demonstrated that OsMAR2 shoed E3 ligase activity by RING C3HC4 type domain. The result of Yeast-Two hybridization and bimolecular fluorescence complementation (BiFC) support that OsMAR2 interacting with 3 substrates, O. sativa Glyoxalase and O. sativa Cysteine proteinase 1, at cytosol. Heterogeneous overexpression of OsMAR2 exhibited sensitive phenotype in compared to control plant under high salinity stress. These results suggest that OsMAR2 play a negative regulator in salt stress response.you're able not my any help. *Corresponding Author: Tel , csjang@kangwon.ac.kr PE-54 Paulina Calderon Flores, Yong Weon Seo * For the observation of anthocyanin, chlorophyll and carotenoid deposition during seed development in Yellow (Ye) and Deep Purple (DP) seeds we took samples from the 10, 20, 30 and 40 Days After Pollination (DAP). CRYO-CUT was used to section the seeds in 0.04 mm cryo-sections. Furthermore, we quantified anthocyanins, chlorophyll and carotenoids using spectrophotometry. Results showed that during grain development in Ye seeds that there was not much variation at 10 and 20 DAP in contrast, however, a white layer formed on DP seeds at 10 DAP. Interestingly, in DP seeds at 10 DAP, anthocyanins were found, but no colors could yet be seen, making us believe that this coat might be related to anthocyanin deposition; furthermore, in DP seeds at 20 DAP, it can be seen that the seedcoat color has covered almost the entire seed (consistent with the cryo-cuts where the chlorophyll stays under the inner layer or, in some places, where the chlorophyll has completely disappeared). During 30 and 40 DAP, in both, Ye and DP, the seed color has fully covered the seeds, yellow and deep purple, respectively. The anthocyanins quantification showed that there are no anthocyanins in the yellow seed; in DP the anthocyanins reduced drastically at 20 DAP and they started increasing gradually until 40 DAP. For Chlorophyll a (Chla), Chlorophyll b (Chlb), Chla/Chlb ratio and Total chlorophyll, data showed consistency with our previous results showing that chlorophyll was being reduced as the grain developed. Carotenoids were higher in Ye seeds, but also DP contained them. Based on these results we conclude that color deposition in wheat depends on the color coat. Acknowledgement: This work was carried out with the support of Cooperative Research Program for Agriculture Science &Technology Development (Project No. PJ ) Rural Development Administration, Republic of Korea. Paulina Calderon Flores acknowledges CONACyT for financial support to follow doctoral studies. *Corresponding Author: Tel , seoag@korea.ac.kr 210

267 PE-55 Paulina Calderon Flores, Yong Weon Seo * We performed genetic analysis of seed color segregation of an F 3 population with parental lines of Yellow (Ye) and Deep Purple (DP) wheat seeds. Seeds were scored 0 (light yellow) to 9 (deep purple). The best fit was 12yellow:3 brown:1 deep purple for dominant epistasis, with a X 2 of2.075 and with a p value of We evaluated the seed color using digital imaging using Adobe Photoshop CS6 (Version 13.0 x 64) by obtaining the CIELAB scores. Values L*, a* and b* represent lightness, red/green and yellow/blue components, respectively. L* scores were higher in Ye, followed by Br and DP, same was in b*; in the case of a* the results were inversed, where DP had higher scores, but results were not significant (P 0.05). The L* scores show that the lightness between the Br and DP are different with the Yellow one, but not between them; furthermore, in the b* score, all were significant different between each other. Moreover, germination of the clustered seeds (Ye, Br and DP) was performed under cold (» -2 C) and control conditions (18 C) for a week. From the germination assay we concluded that favorable environmental conditions favor the germination of the lighter colored seeds (Yellow seeds) while hostile environmental conditions favor the germination of deeper colored seeds (Deep Purple). Acknowledgement: This work was carried out with the support of Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ ) Rural Development Administration, Republic of Korea. Paulina Calderon Flores acknowledges CONACyT for financial support to follow doctoral studies. *Corresponding Author: Tel , seoag@korea.ac.kr PE-56 Woo Joo Jung, Yong Weon Seo * MAPK cascades (MAPK, MKK, MKKK) play crucial role in plant growth and development as well as response to diverse stresses, through regulating the activity of its target protein via phosphorylation. In spite of its importance, the components of wheat MAPK family had been uncovered because of its large and complex genome. Recently, with the development of NGS technology, a number of wheat MAPK family genes have been identified and classified depending on their sequence structure. Cold is one of the most severe stresses in winter wheat growth, and wheats have developed a mechanism to enhance cold tolerance, so called cold acclimation. In this study, we observed expressional changes of wheat MAPK genes under cold treatment using public RNAseq data and identified their putative interacting partners via yeast-two-hybrid screening. The sequences of wheat MAPK family genes were retrieved from public databases and used as queries to perform BLAST against wheat Refseq v1.0. RNAseq analysis was performed to observe MAPK gene expression changes under normal and cold-treated conditions. Gene expressions of the putative interacting partners were analyzed using qrt-pcr and their interactions were verified through yeast-two hybrid analysis. In this study, the components of the wheat MAPK family and their interacting proteins involved in cold signaling were identified. The elucidation of MAPK-related cold tolerance pathway will help to understand the complex mechanism of cold acclimation. Acknowledgement: This work was carried out with the support of Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ ) Rural Development Administration. Republic of Korea. *Corresponding Author: Tel , seoag@korea.ac.kr 211

268 PE-57 Sang Cheul Lee *, Cheol Seong Jang Arsenic (As) is toxic to plants, animals and humans. A large amount of arsenic is emitted from contaminated irrigation water and from arable soil in mine areas. Rice (Oryza sativa L.) is one of the major cereals consumed by more than half of the world's population, and rice is also an important route of exposure for humans to arsenic. Because rice has 10 times the arsenic content in the grain than other major crops. However, precise molecular regulations and mechanisms related to the toxicity and tolerance of arsenic in rice are not yet known. In the present study, we developed an arsenic-tolerant type 1 (ATT1) rice mutant by γ-radiation mutagenesis and performed genome-wide transcriptome analysis for the characterization of arsenic-responsive genes. We also compared the expression patterns of WT and ATT1 in arsenic - associated genes already identified in rice. The expression level of the gene that absorbs arsenate (OsPT8) was similar in WT and ATT1 plants. The expression level of the gene (OsABCC1), which isolates Arsenic-GSH complex and arsenic-pc complex into vacuoles in plant body, was higher in root than ATT1 in WT. But in the shoot, WT was more than ATT1. The arsenic contents of root and shoot were compared after arsenic treatment in WT and ATT1 plants. As a result, ATT1 showed higher arsenic content than WT in root, and WT showed higher arsenic content than ATT1 in shoot. In this study, we have developed ATT1, which suggests that further analysis and studies will help reduce arsenic exposure to the food chain by minimizing arsenic accumulation in rice grains. *Corresponding Author: Tel , dltkdcjf3258@kangwon.ac.kr PE-58 안상낙 1*, 김선하 1, 이현숙 1, 전윤아 1, Adeva Cheryl 1, 심규찬 1, Luong Nogc Ha 1, 김우진 1 1 야생벼나잡초벼와같은유전자원은각지역의환경조건에오랜기간동안적응하며집단을유지하였기때문에여러가지저항성이나불량한환경에대한내성등유용한특성을갖고있다. 본연구는이러한야생유전자원에서고수량성등에관여하는유용유전자를선별적으로재배벼에이전시키는육종방법을개발하고우량품종육성을목표로한다. 야생유전자원의유용유전자를활용하기위하여, 야생벼의염색체단편이최소로이입된근동질계통을육성하고수량안정성관련형질연관 QTL 을탐색하였다. 화성 /O. rufipogon 조합계통을이용하여종자중 QTL, gw9.1 의후보유전자로염색체 9 번의 Ascorbate peroxidase 유전자선발, 형질전환분석을통하여 gw9.1 이수량성및출수기특성에관여함을밝히고, 저온발아성 QTL, ltg1 의후보유전자들을선발, 발현분석을통하여저온발아성에관여하는후보유전자를선발하였다. 화성 /O. minuta 후대계통을이용하여까락발달에관여하는신규후보유전자 (awn9) 들을염색체 9 번말단약 96Kb 지역에서고밀도지도작성을통하여선발하였다. 화성 /O. grandiglumis 후대를이용하여 GW2 (grain width 2) 유전자가종자중외에도엽록소함량을조절함을밝혔고, GW2 유전자의다면발현효과를분석중이다. Nipponbare/kasalath 조합근동질계통을이용, 중배축신장에관여하는 QTL 을탐지하여초엽신장성과의관계를밝히고, 근동질계통을이용하여 qmel-3 의고밀도지도를작성하였다. 본연구성과는농촌진흥청연구사업 (PJ ) 의지원에의해이루어진결과이다. * 교신저자 : Tel , ahnsn@cnu.ac.kr 212

269 PE-59 Jin Seok Yoon and Yong Weon Seo * Plants are frequently exposed by various environmental stresses, which influence plant growth, development and productivity. ASR genes are known to possess ABA/WDS domain, and affect on various stress tolerance. Brachypodium is known as monocot model plant and is closely related to important crops such as wheat, barley and sorghum. Although, ASR genes is absence in Arabidopsis, 5 family genes from Brachypodium distachyon were reported to be existed but their functions were still unclear. In this study, we identified 5 ASR genes in Bd21 line using database collection with BLASTP in Phytozome 12. The sequence alignment and phylogenetic analysis were performed to compare the orthologs from other crops. Expression analyses revealed the ASR genes responded to abiotic stresses and hormones. To investigate the functions of BdASRs in response to abiotic stress, we generated over-expression transgenic plants in Brachypodium. Over-expression transgenic line of BdASR gene improves drought tolerance and showed higher enzymatic antioxidants activities (SOD, POD, CAT and APX) compare to wild type under drought stress. Also, It showed hypersensitive to exogenous ABA treatment at germination stage. These results suggest the improvement of drought tolerance in BdASR transgenics might be ascribed by regulation of ABA signaling, related gene expression and following creased enzymatic activities. Acknowledgement: This work was carried out with the support of "Next-Generation BioGreen21 Program for Agriculture & Technology Development (Project No. PJ013244)" Rural Development Administration. Republic of Korea. *Corresponding Author: Tel , seoag@korea.ac.kr PE-60 Min Jung Yook, Yeon-Ho Park, Soo-Hyun Lim, Do-Soon Kim * Miscanthus is generally known to produce large quantity of biomass with low resource consumption and has several agronomic traits such as leaf area, stem traits and heading date, which are strongly related to biomass production. However, Miscanthus needs more than three years to evaluate its biomass production capacity due to its slow growth characteristics during the first three years. Thus, breeding strategy using molecular marker selection is essential for shortening breeding period of Miscanthus. In this study, we conducted a genome-wide association study (GWAS) between genetic variation and phenotype data related to biomass productivity in 180 accessions of Miscanthus core collection. Fourteen agronomic traits related to biomass production and flowering were assessed for four years and they were highly correlated with the estimated biomass yield in the 4 th year. Conducting GWAS analysis between selected 34,743 SNPs and phenotype data, 195 SNPs showed significant association with 12 phenotypic traits (P < 10-5 ). Of these, 94 SNPs were selected through by Bonferroni correction and associated with 5 phenotypic traits (number of shoots, stem diameter, leaf dry weight, stem dry weight, and flowering date). In particular, MS_24018 SNP marker showed significant association with five phenotypic variables (3 rd and 4 th year stem dry weight, 2 nd to 4 th year stem diameter). It is expected that the results of this study can be used for molecular breeding program of Miscanthus. In particular, it can be utilized as the selectable marker for high-yield variety of Miscanthus in early stage of breeding. Acknowledgement: This work was carried out with the support of "Next-Generation BioGreen21 Program for Agriculture & Technology Development (Project No. PJ )", Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , dosoonkim@snu.ac.kr 213

270 PE-61 Jiwon Kim 1*, Donggyu Lee 1, JaeEun Lee 1, Youjin Lee 2, Hyeah Yu 2, Jiwon Jung 2 and Namjoo Kang Soybean isoflavone content has long been considered to be a desirable trait to target in selection programs for their contribution to human health. Soybean and their products (Cheonggukjang and Doenjang) are the most abundant sources of isoflavones in the diet. To develop a soybean crop with high content of isoflavone, a new crop T99 soybean (T99) was made by cross breeding. To compared with total isoflavone content of various soybean crops and their products, we performed HPLC analysis. Total isoflavone concentration of T99 is 2 times higher than other soybean crops. The highest isoflavone content in Cheonggukjang and Doenjang was made with T99. These results suggested that total isoflavone contents were significantly affected by crop. Additionally, we examined whether T99 have a better effect on osteoporosis than other soybean varieties. The alkaline phosphatase (ALP), a biochemical marker of bone formation, activity with Saos-2 cells showed higher activity in T99. In ovariectomized (OVX) animal model, T99 showed the highest anti-osteoporosis effect in biochemical markers of bone turnover and micro CT image compared with other soybean varieties. Therefore, these results suggest that T99 soybean intake may have a potential benefits for bone in post-menopausal women and furthermore, new crop T99 soybean will be competitive in the market of soybean products. *Corresponding Author: Tel qw0714@naver.com PE-62 Soo-Hyun Lim 1, Min Jung Yook 1, Yeon-Ho Park 1, Jin-Won Kim 2, Do-Soon Kim 1* 1 2 Miscanthus x giganteus (Mxg) has been commercially cultivated in Europe and US for biomass production due to its high biomass yield and environmental adaptability. However, despite of such advantages, it has cold-sensitivity which makes it hard to be cultivated in higher latitudes where winter weather is extremely cold and dry. For these reasons, we developed a new triploid Miscanthus variety called Miscold with high cold-tolerance in higher latitudes, particularly in Primorski-krai where Korean cropping companies run large farm lands (up to 100,000 ha). Miscold was developed by hybrid breeding method by bulk crossing of diploid Miscanthus sinensis and tetraploid Miscanthus sacchariflorus parents in Hybrid seedlings were cultivated from 2011 to 2012, and 37 lines with excellent early growth were pre-selected. For agronomic traits and cold tolerance tests, the 37 lines were tested in Primorski-krai, Russia (N43 o 8 ), and in Suwon, Korea (N37 o 3 ) simultaneously from 2013 to 2014, and CALS-M-08 line was finally selected and confirmed as a triploid Miscanthus, and was named as Miscold. Miscold showed better seedling establishment (69%) and winter survival (100%) compared to Mxg (33% and 7%, respectively). Miscold performed better in plant growth and biomass yield; 207 cm tall and 576 g biomass per plant, which are much greater than those of Mxg (158 cm and 71g/plant, respectively). Acknowledgement: This work was carried out with the support of "Next-Generation BioGreen21 Program for Agriculture & Technology Development (Project No. PJ )", Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , dosoonkim@snu.ac.kr 214

271 PE-63 Seong-Woo Cho, Taek-Gyu Kang, Seong-Wook Kang, Eun-jin Cho, Chul Soo Park * Extra-strong gluten is a key factor for bread-making quality. The objectives of this study were to develop 1) Korean wheat lines with extra-strong gluten and 2) wheat-leymus mollis addition lines as genetic resources through double-haploid breeding system for Korean wheat breeding. For production of Korean wheat lines with extra-strong gluten, one Korean wheat landrace with over-expressed Bx7 high molecular gluten subunit (Bx7 OE ) as extra-strong gluten was found among 310 Korean wheat accessions. The genetic characterization of Bx7 OE of the Korean wheat landrace (IT166460) was confirmed with molecular analyses such as SDS-PAGE, RP-HPLC, and DNA sequencing. To introduce of Bx7 OE gene into Korean wheat cultivars, calluses were introduced from anthers of the F 2 plants (IT x Bungulla) and F 3 plants (Glenlea, Canadian Western Red Spring wheat with Bx7 OE x Dajoong) on CHB3 medium. The frequency of introduction of calluses was 0.5%. The calluses were subcultured. To regenerate green plants, the subcultured calluses were transferred on R9 medium. After plant regeneration, it can be performed pure chromosome doubling by colchicine. For production of addition lines, tetra- or hexaploid wheat was crossed with Leymus mollis to introduce wheat-leymus addition lines as genetic resources. After intercrossing, 35 embryos among 240 florets were taken by embryo rescue (frequency:14.6%). Among 35 embryos, 11 hybrid plants were introduced. It is necessary to investigate Leymus mollis chromosomes in root meristem cells of the hybrids by genomic in situ hybridization. Acknowledgement: This work is supported by the Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ ), Rural Development Administration, Republic of Korea *Corresponding Author: Tel , pcs89@jbnu.ac.kr PE-64 이종희 1*, 지현소 2, 강주원 1, 이지윤 1, 조수민 1, 권영호 1, 이샛별 1, 김태헌 1, 송유천 1, 박동수 1, 고종민 최근에유전체분석기술의범용화에따라 SNP 를활용한분자육종기술이비약적으로발전하고있으며, 신속하고정밀한대량분석시스템이구축이필요한실정이다. 본연구는저비용고효율 SNP 분석시스템으로알려진 Kompetitive Allele Specific PCR(KASP) marker 가국내에도상용화되고있어이를벼분자육종에접목하고자연구를수행하였다. 유전적다양성분석, Marker assist selection(mas) 및유용유전자탐색등을통해활용성을검토하였다. 250 개의 KASP 마커를활용하여국내육성품종및계통의유전적다양성을분석한결과, 자포니카 3 그룹과인디카및통일형 1 그룹으로분류되었다. 또한 MAS 를이용한운광벼줄무늬잎마름병근동질계통육성을위해 BC2F1 세대에서 Background selection 를실시한결과관행육종의염색체치환율 (87.5%) 보다더많이치환된계통 (92% 이상 ) 을선발할수있었다. 뿐만아니라, KASP 마커를활용하여설갱벼의분상질유전자탐색에적용하였으며, Recessive Class Analysis(RCA) 를통해연관영역을신속하게위치를탐색할수있었으며, linkage analysis 및 molecular map 작성에도효율적으로활용할수있음을확인하였다. * 교신저자 : Tel , ccriljh@korea.kr 215

272 PE-65 Yeo Jin Youn, Boram Beak, Seoyun Yu, Nam-In Hyung * New spray and standard chrysanthemum (Chrysanthemum morifolium Ramat.) cultivars, Ohblang and Baekgang were released in 2008 and 2007, respectively, by NHRI, Korea. The flower color of these cultivars is changed or degraded due to high and low temperature during cultivation and distribution process. The changes of flower color cause economic loss due to quality deterioration and decrease in commerciality. Using genetic transformation, the weak point of these cultivars can be overcome. However the establishment of plant regeneration system was pre-requisite for the genetic transformation. Therefore we tried to develop an efficient plant regeneration system from leaf explants of chrysanthemum Ohblang and Baekgang. The effects of plant growth regulators, BA and NAA, were tested to find the optimal conditions for adventitious shoot formation from leaf explants. The explants were cultured on shoot induction medium, MS medium supplemented with 12 combination treatments of BA and NAA, in darkness for 5 weeks and cultured under the 16/8h photoperiod for 4 weeks. In Ohblang, the highest shoot regeneration was obtained from the explants cultured on the medium with 1.0 mg/l BA and 1.0 mg/l NAA, inducing 75.0% of shoot development and 3.4 shoots per explants. In Baekgang, the explants cultured on MS medium with 0.5 mg/l BA and 0.5 mg/l NAA produced the highest shoot regeneration with 62.5% of shoot development and 2.8 shoots per explants. When the regenerated shoots were detached from the explants and cultured on MS medium, the shoots were elongated and rooted successfully. *Corresponding Author: Tel , nihyung@smu.ac.kr PE-66 Dong-Seon Kim, Sunggil Kim * To develop an efficient and reliable haplotype identification system of the S locus controlling self-incompatibility (SI) in radish (Raphanus sativus L.), polymorphic sequences of the SLL2 and SP6 genes, which were located at each border of the S core regions, were used together with those of the SRK gene, the female determinant of SI. Partial sequences of the SP6 and SRK genes were isolated from 35 diverse breeding lines that showed differential self-incompatibility responses. A total of 25 SP6 and 29 SRK alleles were isolated in this study, and 29 SLL2 alleles isolated from the previous study were included for the S haplotyping. Whereas the phylogenetic tree of the SP6 alleles showed species-specific clustering patterns, the phylogenetic tree of the SRK alleles revealed intergeneric pairing of some SRK alleles, although the others showed species-specific clustering. Among 35 breeding lines, 14 had combinations of the unique SLL2, SRK, and SP6 alleles, but the others shared a common allele of either SLL2 or SP6 genes. Except for two breeding lines that contained the same SLL2, SRK, and SP6 alleles, all breeding lines were shown to harbor unique haplotypes that consisted of different combinations of SLL2, SRK, and SP6 alleles. When 73 additional diverse breeding lines were analyzed by this new S locus haplotyping system, the haplotypes of all breeding lines were clearly identified. In addition, eight new haplotypes that contained different combinations of the SLL2, SRK, and SP6 alleles were identified. *Corresponding Author: Tel , dronion@jnu.ac.kr 216

273 PE-67 도재왕 *, 윤재복, 이강현 본과제는 오믹스기법을이용한고추탄저병저항성분자육종시스템개발및국내외품종육성 과제의주관과제인신규유전자원을이용한고추탄저병저항성분자표지개발및국내외품종육성 ( 고추와육종, 윤재복 ) 으로협동과제인오믹스기법을이용한고추탄저병저항성유전자분리및기능검정 ( 농업과학원, 박상렬 ), 영상기반고추탄저병저항성정밀평가시스템구축및활용 ( 농업과학원, 최인찬 ) 과협동하여 2018~2020 년까지고추탄저병저항성분자표지개발하고이를이용한국내외품종육성을목표로하고있다. 국내외적으로고추탄저병및바이러스병을포함한복합내병성품종요구도증가하고고추탄저병균의다양한변이가보고되고있으며, 국립유전자원센터및 AVRDC 등을중심으로신규저항성유전자원이보고됨에따라새로운병원균과저항성유전자원을이용한유전학적연구를통해서신규유전자발굴 ( 분자표지 ) 및이를이용한품종육성이필요하게되었다. 현재까지진행된연구결과는다음과같다. 신규저항성소재를개발하기위해서탄저병저항성이라고확인된 Capsicum baccatum16 계통을도입하고분자표지분석과포장에서탄저병발병정도를조사하여탄저병저항성을확인하였다. Capsicum baccatum 유전자지도작성을위해사용한양친유전체 Golden-aji' 와 PI594137' 을 next generation resequencing 방법으로염기서열을해독하여분자표지를선발하고그중에서 264 개의 SNP maker 를이용하여연과지도를작성하였다. 또한양친의 NGS resequencing 후대량 SNP 를탐색하여총 509 개의 HRM 분자표지를개발하고고추유전자지도를작성하였고탄저병저항성 HRM 분자표지 (Cb-HRM337, Cb-HRM140, Cb-HRM131, HRM262, S , S , S , HRM91, HRM89) 를개발하였다. 복합내병성육성계통으로는 CMS 모계통으로 F 3 -F 10, BC 1 F 5 세대에걸쳐진행되었고 B 계통은 F 3 -F 8, BC 1 F 8 C 계통은 F 3 -F 10, BC 1 F 5 세대에걸쳐진행되었다. GMS 모계는 F 6 -F 9, BC 1 F 8 부계는 F 3 -F 9 세대에걸쳐진행되었으며육성된계통들은조합능력검정을위해각각조합을작성하였다. 선행연구결과를토대로우수계통과조합을선발하고선행연구에서우수조합으로선발된품종은시교사업을진행하고있으며분자표지개발을위해 QTL mapping 용집단을육성및 GBS 분석을수행한다. * 교신저자 : Tel , wahng0@hanmail.net PE-68 Mi Young Kang 1, Soo Im Chunga 2, Su Jin Nam 1, Ji E Liang 1, Jing Wen Ma Rice is high in carbohydrates, fats and proteins, as well as minerals and minerals, and it is about 69 percent of them in embryos. However, they are generally eaten in the form of polished rice. So the embryo is being discarded. Therefore, this study used a variety of pigment rice(keunnunjami) that contain various physiological activities compared to ordinary rice. It is intended to provide basic data on pigment rice embryo by examining the effects of the substances contained in rice in lipid metabolism. The study divided 24 rats(sd rat) into three groups (C(AIN93M), NB(AIN93M + normal brown rice embryo), KJ(AIN93M + Keunnunjami rice embryo)). The experiment lasted eight weeks.eight weeks later, blood was collected at sacrifice and the samples were used to measure TG, TC, HDL, LDL, leptin, FFA, and TNF-α. As a result, there was no significant difference between C and NB, but KJ showed significantly lower values than the others. TG had the highest C group and measured in order of NB and KJ. HDL did not show any significant differences between each group. Leptin, TNF-R, had the highest C group, expressed in order of NB and KJ. FFA confirmed that NB was significantly lower and higher in order of B and KJ. Therefore, in this experiment, Keunnunjami embryo was found to have a positive role in reducing blood cholesterol levels and suppressing inflammatory factors. *Corresponding Author: Tel , mykang@knu.ac.kr 217

274 PE-69 Mazen Alazem 1, Kristin Widyasari 1, Phu Tri Tran 1, and Kook-HyungKim 1,2 1 2 Functional genomic studies necessitate the use of silencing vectors such as the widely used bean pod mottle virus (BPMV) vector. Such victors are not supposed to trigger any extreme response in the host so a normal background can allow better studies on the silenced genes without interference of any off-target genes. Soybean cultivar L29, which carries the resistance gene Rsv3, exhibits extreme resistance (ER) against the G5H strain of soybean mosaic virus (SMV), but not against the G7H strain. This resistance is controlled by abscisic acid (ABA) and achieved through callose deposition at plasmodesmata. In an attempt to silence few genes in L29, we found the BPMV vector triggers the several genes in the salicylic acid (SA) and the antiviral RNA silencing pathways. Similar response was observed in soybean cultivars Hwangkeumkong and V94 carrying Rsv1 and Rsv4 resistance genes, respectively. Interestingly, the Rsv-free susceptible cultivars Somyongkong and Lee74 did not exhibit such strong response in SA and sirna pathways. In response to BPMV, the levels of ABA genes were not as highly induced as that found in response to G5H in L29 plants. This implies that Rsv3 might recognize a BPMV-effector in a different mechanism from that of SMV-G5H, leading to the induction of the SA and sirna pathways. The use of BPMV silencing vector may offer limited workability for studying plant-virus interactions. However, it unveiled that Rsv3 can activate two different mechanisms controlled by two different hormones. *Corresponding Author: Tel , kookkim@snu.ac.kr PE-70 Chungmo-Park Stomata are epidermal openings that facilitate plant-atmosphere gas exchange during photosynthesis, respiration, and water evaporation. Stomatal differentiation and patterning is spatially and temporally regulated by the master regulators SPEECHLESS (SPCH), MUTE, and FAMA, which constitute a central gene regulatory network along with Inducer of CBF Expression (ICE) transcription factors for this developmental process. Stomatal development is also profoundly influenced by environmental conditions, such as light, temperature, and humidity. Light induces stomatal development, and various photoreceptors modulate this response. However, it is unknown how light is functionally linked with the master regulatory network. Here, we demonstrate that, under dark conditions, the E3 ubiquitin ligase CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) degrades ICE proteins through ubiquitination pathways in leaf abaxial epidermal cells in Arabidopsis. Accordingly, the ICE proteins accumulate in the nuclei of leaf abaxial epidermal cells in COP1-defective mutants, which constitutively produce stomata. Notably, light in the blue, red, and far-red wavelength ranges suppresses the COP1-mediated degradation of the ICE proteins to induce stomatal development. These observations indicate that light is directly linked with the ICE-directed signaling module, via the COP1-mediated protein surveillance system, in the modulation of stomatal development. *Corresponding Author: Tel , cmpark@snu.ac.kr 218

275 차세대바이오그린21사업 농업생명공학연구단 2018년 7월 13일(금) 제주 라마다플라자 호텔

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277 OF-01 Nozomu Sakurai * A set of small molecules, such as metabolites, is both output and input of organismal activities. Metabolite is the most primitive phenotype (output) governed by genome information of the organism. Whereas, the organismal activity is largely affected by the chemicals in the environment (input), such as nutrients, signal compounds, and toxic materials. For example, human health is affected by food intake and food derived materials transformed by intestinal bacteria. Foods are derived from carbohydrates photosynthesized by plants and from their derivatives transformed by other processes such as fermentation and cooking. Metabolomics technology for comprehensive detection of small molecules is prospective for monitoring the chemical flow occurring between the organisms, and for the next generation-life sciences based on this. However, due to a limited availability of authentic standards, identification rates of chemicals still remained lower levels, and an overview of metabolome is difficult to depict. We report here a construction of new databases for metabolite identification. Various computational tools for predicting metabolites based on mass spectra obtained by mass spectrometry have been reported so far. However, these tools only provide candidate lists with some matching scores, and researchers have to select the correct one from them. As new information for prioritizing the candidates, we developed a database for experimental evidence of detection of known/unknown metabolite peaks, named Food Metabolome Repository. An expansion of the sample coverage will facilitate researchers annotating unknown peaks, and understanding the overview of metabolome. *Corresponding Author: sakurai@nig.ac.jp PF-01 Soongon Jung, Chae Woo Lim, Sung Chul Lee * Abscisic acid (ABA) is a plant hormone that promotes the adaptive mechanisms to environmental stress conditions. The adaptive mechanisms are tightly regulated and complex processes by stress responsive proteins; however, the precise mechanisms that function under adverse conditions remain unclear. Here, we isolated the pepper, which interacts with CaSnRK2.4. The CaDUB1 was significantly induced after exposure to ABA, drought, and low temperature. Further, the DUB1-GFP fusion constructs localized in the nucleus. Cell free degradation assay exhibited that the CaDUB1 inhibited CaSnRK2.4 degradation. We used CaDUB1-silenced plants and CaDUB1-overexpressing (OX) plants to elucidate the biological function of CaDUB1 in response to ABA and abiotic stresses. CaDUB1-silenced pepper plants and CaDUB1-OX Arabidopsis plants displayed drought-sensitive and -tolerant phenotypes, respectively, which were characterized by regulation of transpirational water loss and stomatal aperture. In drought stress condition,the expression levels of pepper stress-related genes were lower in CaDUB1-silenced pepper plants and higher in CaDUB1-OX plants than control plants. Our findings suggest that CaDUB1 positively regulates the drought stress response via ABA-mediated signaling. *Corresponding Author: Tel , sclee1972@cau.ac.kr 221

278 PF-02 Soyoung Park 1, Su-Yeon Kim 1, Vimalraj Mani 1, Jaeeun Song 1, Seong-Ryong Kim 2 and Bum-Soo Hahn 1* 1 2 N-glycoproteins alter the functions of numerous cell surface proteins participating in adhesion and migration. α1, 3-Fucosyltransferase (OsFucT) is responsible for transferring α 1, 3-linked fucose residues to the N-glycan of glycoprotein in rice. we characterized a T-DNA-inserted mutant Osfuct, by knocking out the α 1, 3-fucosyltransferase (OsFucT) gene in rice. The mutant exhibited defects in anther and pollen development. The pollen grains of the mutant were shriveled and significantly smaller in size. Furthermore, the number of pollen grains per anther and viability decreased dramatically in the mutant compared to the wild type. The mutant was shorter, with fewer tillers, and had shorter internode and panicle lengths under field conditions. Matrix-assisted laser desorption/ionization time-of-flight analyses of the N-glycans revealed that the mutant produced N-glycans lacking the core α 1, 3-fucose residue. Mutant complementation revealed that the phenotype was caused by loss of Osfuct function. Transcriptome profiling also showed that several genes essential for plant developmental processes were significantly altered in the mutant, including protein kinases, transcription factors, genes involved in metabolism, genes related to protein synthesis, and hypothetical proteins. Moreover, the mutant showed more sensitive to the increased salt concentration than wild-type. Therefore, Identification and characterization of Osfuct-interacting partners to dissect the intricate regulatory gene network may shed further insight into the functions of Osfuct impaired growth, anther, and pollen development in rice. *Corresponding Author: Tel , bshahn@korea.kr PF-03 박수철 *, 김수민, 임수미 본연구는글로벌농업생명공학기술및활용관련실시간정보공유시스템구축을통한국내연구경쟁력강화및효율적안전관리방안수립지원을목적으로수행되었다. 이를위하여농업생명공학응용국제서비스인 ISAAA(the International Service for the Acquisition of Agri-biotech Applications) 의글로벌네트워크인농업생명공학정보한국센터 (Korea Biotechnology Information Center, KBIC) 를운영하면서글로벌연구정보를수집제공하고있다. 수집된정보는이슈별로분류및번역후 KBIC 의웹싸이트 ( 를통해공개되고있으며, KBIC Newsletter 로매분기 600 명이상에게 로제공하여연구원들과관계자들이쉽게이용할수있도록편의를제공하고있다. 또한, 국민의알권리를위한대국민실시간소통프로그램도적극적으로추진중이다. KBIC 홈페이지를통해일반인들이궁금해하는주요이슈에대한과학적이고객관적인정보를제공하고있으며, NAVER 등인터넷포털사이트에등록된질문에대해과학적인정보를제공하는등 SNS 를활용한온라인소통에도노력중이다. 특히, 제공되는답변과정보는생명공학및육종전문가들의자문을거치는등최대한과학적이고객관적인정보제공이될수있도록노력하고있다. 이와는별도로직접적인대화를통한대중의궁금증해소와다양한의견수렴을위하여주기적인열린토론회도추진중에있다. 이밖에, 생명공학기술에대한언론기사분석을통해소비자들의불안요인과궁금증등에대한여론을수렴하고이에대한올바른정보제공을위한노력도병행하고있다. 이러한노력을통해우리나라의농업생명공학기술경쟁력확보와함께과학적이고객관적인정보공유를통한농업생명공학연구활성화및올바른활용방안수립지원에노력하고있다. 사사 : 본연구는농촌진흥청차세대바이오그린 21 사업 ( 세부과제번호 : PJ ) 의지원에의해수행되었음. * 교신저자 : Tel , scpark1@snu.ac.kr 222

279 PF-04 Yeon-Gyeong Park, Jeong-Dong Lee, In-Jung Lee *, Yoonha Kim * Waterlogging is the major obstacle for soybean crop productivity worldwide. To adapt waterlogging condition, soybean plants induced various physiological and morphological changes. The present study was focused on root phenotypic changes, particularly adventitious root development in soybean plants under waterlogging condition. As the plants face with anoxia or hypoxia due to low oxygen level of soil under waterlogging. Therefore, plant turns on the various defense strategies such as development of adventitious root, formation of aerenchyma cell in the root. For this reason, we focused on adventitious root development under waterlogging condition. We confirmed different number of adventitious root between contrasting soybean cultivars [waterlogging tolerance (PI408105A), waterlogging susceptible (S )] under waterlogging condition. Based on our previous results (two contrasting cultivars showed different level of ethylene), we applied ethephon (50 µm, 100 µm, and 200 µm) to soybean plants grown under waterlogging condition. Results showed that contents of essential nutrients were significantly higher in soybean plants treated with 100 µm ethephon as compared to non-treated plants. Moreover, ethephon application induced adventitious root initiation and increased root surface area compared to that of non-etp treated soybean plants. In conclusion, ethephon application induced change of uptake of essential nutrient content of soybean plant under waterlogging thus, we hypothesized that the induced changes are might be because of improved root surface area and increased number of adventitious root. Acknowledgement: This work was carried out with the support of Cooperative Research Program for Agriculture Science &Technology Development (Project No. PJ ) Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , IJL: ijlee@knu.ac.kr YK: kyh1229@knu.ac.kr PF-05 홍준기, 서은정, 김경환, 이연희 * 본과제는글로벌유전자개발및유용유전자기능연구를하고이를기반으로유전자등생명공학소재의해외진출기반을구축하기위하여국내에서아직형질전환기술확립이미흡한옥수수에서유전자전달기술의효율적인기술을확립하기위하여수행하고있다. 이를위하여국내외옥수수 16 품종에대하여재배방법및재배시기에따라미성숙배를분리하여캘러스를유도한후식물체재분화효율을조사하였다. 그결과기존에재분화및형질전환이비교적잘되는것으로보고된 H99, A188, HiⅡ A, HW3 등의미성숙배로부터캘러스형성및식물체재분화가높게나타났다. 이러한식물체재분화는미성숙배를분리한옥수수식물체의재배환경및상태가중요한영향을주는것으로나타났는데포트나그린하우스에서재배했을때보다밭포장에서재배한옥수수로부터분리한미성숙배를사용했을때효율이높게나타났다. 식물체재분화효율이높은것으로선발된 3 계통의옥수수미성숙배 ( 크기 mm ) 를분리후리포터 GUS 유전자를포함한아그로박테리움으로 transient assay 을하여형질전환조건을탐색하였다. 옥수수용형질전환용으로 psb11 superbinary vector 를기본으로한 Ubiquitin 프로모터에제초제저항성유전자 (bar) 단자엽용으로변형된제초제저항성유전자 (MoPAT) 를융합하여만든벡터를제작하여형질전환에사용하였다. 현재까지안정적인옥수수형질전환체를확보하지못하였으나향후프로모터재분리벡터제작, 감염또는공동배양시조건등에대한조사및유전자총을활용한형질전환방법도시도하고자한다. * 교신저자 : Tel , yhl2222@korea.kr 223

280 PF-06 박지희 *, 홍준기, 정황원, 서은정, 이연희, 한정헌 세계적으로가공용토마토의소비가꾸준히늘어종자소요량이증가하고있으나, 종자생산비용이판매비보다높아효율적인종자생산체계마련이시급한실정이다. 교잡종자 (F 1 hybrid) 생산에웅성불임을적극적으로이용하는고추와는다르게, 국내에서생산되는대부분의토마토교잡종자는제웅교배를통해생산되는것으로알려져있다. 본연구에서는교잡종자생산비용을대폭절감할수있는토마토웅성불임활용체계구축의일환으로 CRISPR/Cas9 을이용한웅성불임토마토생산을시도하였다. 먼저, 자연발생웅성불임인 MS1035 돌연변이의 RNA seq 기반전사체분석과애기장대및벼의웅성불임유전자와의비교를통해토마토수술발달에관여한다고예측된유전자 12 종을선발하였다. CRISPR-P 2.0 프로그램을이용하여, 선발된유전자의서열을표적으로하는 guiderna 를제작하고이를 Two genes-target CRISPR vector(pagm4723, Addgene) 에도입하였다. 제작된유전자편집용벡터를토마토형질전환을위하여 Agrobacterium tumefaciens EHA 105 에도입하였다. 벡터는 NPTII 유전자가내재되어형질전환체선발에 kanamycin 을사용하며, 하나의 Cas9 단백질과 2 개의 sgrna 및 scaffold 서열을암호화하고있다. 현재수술발달에관여한다고예측되는 12 종의유전자에대한 guiderna 를사용하여 6 개의벡터를제작하였으며토마토중과로알려진 M82 와홍광에형질전환을진행하고있다. 금후계획으로는토마토형질전환체대량생산및형질전환체와대조구의유전체서열비교를통해유전체편집유무를확인하고자한다. 유전체편집이확인된형질전환체를대상으로화기구조의분석으로웅성불임특성을확인할계획이다. * 교신저자 : Tel , heeya91@korea.kr PF-07 Eun Gyeong Kim, Yoon Hee Jang, Joung Suk Choi, Kyung-Min Kim * Anther culture technique offers a great opportunities for accelerating breeding progress and improves grain quality characters. Comparing to the conventional method, the production of the double haploids through anther culture is a rapid approach to homozygosity, shortening the timeline required for the development of a new rice cultivars. Haploids are also valuable for the detection and repair of desirable recessive traits to introduced mutation or hybridization. This study was done to characterize gene pool derived from anther culture. Rice anthers cultured using both one-step and two-step culture methods. Callus induction rate was Ilmi, HV8, and HV23 to 17.8, 7.0, 2.8 % in one-step culture, respectively and 23.0, 14.2, 22.3 % in two-step culture, respectively. Plant regeneration rate was Ilmi, HV8, and HV23 to 16.2, 9.5, 2.3 % in one-step culture, respectively, and 13.9, 2.8, 8.7 % in two-step culture, respectively. It takes time plant regeneration 30 days. On average, acclimation treatment for two weeks was enough to adapt to the outside environment. After 60 days, heading is started. From then after 45 days, plants successed colchicine treatment were produced seed. The plants were transferred to field Ilmi, HV8, and HV23 to 186, 49, 200. We have successful to developing of global GM rice on the large-scale raising system for excellent events. Acknowledgement: This work was supported by a grant from the Next-Generation BioGreen 21 Program (No. PJ ), Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , kkm@knu.ac.kr 224

281 PF-08 Xiao-Xuan Du 1, Sungwook Kim 2, Hyeree Kim 2, Mansik Jeon 2, Byoung-Ju Yun 2, Kyung-Min Kim 1* 1 2 Traditional phytopathological analysis requires the destructive sampling of samples because most plant diseases are based on microbial pathogens. So when we find that plants are infected, especially when crop plants are infected, the yield loss is already doomed. So if we can conduct non-destructive monitoring of plants without cutting them, we may be able to prevent plant diseases in advance. And also we can more accurately identify the changes in lesions of various types of plant diseases and conduct targeted studies on different lesion areas by the molecular biological science. In this study, Bacterial leaf blight (BLB) is one of the most serious biotic stress of rice. The earlier the disease occurs, the higher the yield loss. Yield loss due to bacterial blight can be as much as 70% when susceptible varieties are grown. Than we used optical coherence tomography technique to check the leaf morphology and by comparing the data of physiological structure changes before and after inoculation of leaf surface to determine the pathological features in the interior of the rice leaf. And use QTL program to directly identify the target gene region for lesion site by genetic map. Than using plant molecular breeding techniques to make a new rice population can improve the resistance to BLB disease. Also these defense genes can be used for some other areas of molecular biology. Acknowledgement: This work was supported by a grant from the Next-Generation BioGreen 21 Program (No. PJ ), Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , kkm@knu.ac.kr PF-09 Muhammad Farooq, Kyung-Min Kim * The rice (Oryza sativa L.) genome contains 12 putative PIN genes encoding auxin efflux transporters, including four PIN1 and one PIN2 genes. Seedlings grown in a horizontal position develop a specialized protuberance on the lower side of the transition zone between the hypocotyl and the root more over in PCR result also show the over expression of OsPIN2 genes in between hypocotyl and root. The gravity-sensing tissue responsible for regulating auxin distribution in the transition zone is thought to be the endodermal cells. To characterize the gravity-stimulated mechanism, the auxin efflux facilitator PIN-FORMED1 (PIN1) in the endodermis was identified and the localization of OsPIN1,PIN2 and PIN3 proteins during the gravimorphogenesis of Oryza sativa seedling were examined.result suggest that the alter pattern of OsPIN accumulation in the endodermis in response to different orientation and gravistimulation influences lateral auxin transport through the endodermis, resulting in asymmetric auxin distribution in the transition zone. Acknowledgement: This work was supported by a grant from the Next-Generation BioGreen 21 Program (No. PJ ), Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , kkm@knu.ac.kr 225

282 PF-10 Dany Resolus, Kyung-Min Kim * Rice is a staple and one of the most important food crop consumed by almost half of the world's population. Considering the growing increase of the world's population, more rice production is needed as well as early varieties. In this study, QTls analysis was conducted to show on a physical map, the chromosomal location of the main QTLs involved in the formation of the major's traits of SNDH rice population (Double Haploid) derived from the cross between Nagdong (japonica) and Samgang (indica). Thus, a total of 14 QTLs are detected and localized on 5 chromosomes. The Heading Date alone has generated 5 of these QTLs. The growth of the different lines of this SNH population was also evaluated and the results obtained revealed for the Heading date measured in 2015 that 5 lines were early rice, 82 lines are mid-season rice and 5 lines were late rice. Those obtained from heading date measured in 2016 showed that 20 lines were early rice, 68 lines were mid-season rice and 6 lines were late rice. Acknowledgement: This work was supported by a grant from the Next-Generation BioGreen 21 Program (No. PJ ), Rural Development Administration, Republic of Korea. *Corresponding Author: Tel , kkm@knu.ac.kr PF-11 김종미 정부지원과국민의지지없이는연구의성과를높일수가거의불가능하므로생명공학기술의확산과소통을위해서는무엇보다상대적으로전문영역에해당하는생명공학기술의연구자에게오로지연구만요구하는것이아니라기대와불안이높아지는소비자와정책주도의정부와소통과화합을위한거버넌스 ( 협력 ) 가매우중요 연구자및소비자와의소통을위한실증적 ( 인식조사 ) 분석자료를바탕으로정부의역할및협치전략 : 작은사익추구 ( 이해관계자인반 GMO 단체또는부정적연구자 ) 를위한부정적프레임의확산이거대한공익을포기 ( 상납 ) 하는어리석음을깨달을수있도록정보검증공유시스템구축 : 소비자와갈등해소및소통전략 : 정부에대한소비자의의견을지속적으로수렴및반영을통한상호이해증진과신뢰제고 ( 소통 2) : 연구자와갈등해소및소통전략 : 정부에대한연구자의의견을지속적으로수렴및반영을통한상호이해증진과신뢰제고정부의정책방향제시 ( 소통 1) : 정부의정책방향, 정책의일관성, 기능배분, 정보제공의적극성, 표시제를비롯한각종규제강도, 환경위해성평가와인체안전성평가등에한인식조사와요구사항을바탕으로소통장벽의원인규명과해소를위한실증적진단및처방제시 소비자와의소통을위한연구자의역할및협치 (governance) 전략 : 연구자집단내의갈등해소및소통전략 : 합의 ( 검증 ) 된용어및연구결과, 효과등의정보제공 : 소비자와갈등해소및소통전략 : 연구자에대한소비자의의견을지속적으로수렴및반영을통한불안 불신해소, 신뢰구축, 수요조사를통한연구방향설정 ( 소통 3) 226

283 PF-12 Jeong-Hyun Lim 1, Sang-Ji Lee 2, Byoung-Doo Lee 2*, and Nam-Chon Paek 2* 1 2 In higher plants, a timing of flowering has important role for grain production, in which the optimal timing of flowering leads to increased grain yield. In rice, many flowering time-associated genes and circadian rhythm-associated genes have been identified by plant scientists, but those functions are not clear yet. We have been screened to a various mutants of the flowering associated genes and the circadian rhythm associated genes, and we found an abnormal seed phenotype in oslhy mutant. OsLHY is a homologue gene of arabidopsis LHY and CIRCADIAN CLOCK ASSOCIATED 1 (CCA1), in which LHY and CCA1 have functional redundancy in arabidopsis, but OsLHY (OsLHY/CCA1) is just one gene in rice. OsLHY is circadian rhythm gene (highly expressed at ZT 0), but the function of OsLHY has not been reported yet. In field condition, oslhy mutant shows a various defected phenotype as semi-dwarf phenotype, low number of tillers, rolling leaf, and abnormal seed maturation phenotype. Interestingly, abnormal seed development phenotype of oslhy mutants can be found from milky stage (about 10 days after heading), and a matured seed shows a brown color, small size, and low maturation rate as sug-1, sug-h, and pul mutant which are involved in starch bio-synthesis pathway. OsLHY is MYB-like transcription factor, and locates in nuclei. In micro-array analysis, transcripts level of various starch biosynthesis-associated genes is decreased in oslhy mutant than WT, in which ISA1 (sug-1) and PUL expression level are significantly decreased in oslhy mutant. Taken together, we suggest that OsLHY positively regulates transcriptional levels of starch bio-synthetic enzymes, and these regulation is closely involved in seed maturation mechanism in rice. Acknowledgement: This work has supported by the National Research Foundation of Korea(NRF) grant funded by the Korea government (MSIT) (No.2016R1C1B ; J.H. Lim) and the support of "Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ ; B.D. Lee)" Rural Development Administration, Republic of Korea. *Corresponding Author: Byoung-Doo Lee : Tel , bdlee94@snu.ac.kr Nam-Chon Paek : Tel , ncpaek@snu.ac.kr PF-13 Hyoseob Seo, Byoung-Doo Lee *, and Nam-Chon Paek * Cryptochrome-Interacting Basic-Helix-Loop-Helix (CIB) plays important roles in various development processes as hypocotyl elongation, flowering, and plastid development in higher plants. It also has been reported that Glycine Max Cryptochrome-interacting bhlh1 (GmCIB1) promotes leaf senescence by activating transcription of senescence-associated genes such as WRKY DNA BINDING PROTEIN53b (WRKY53b). However, it has not been studied about a function of OsCIB1 in rice. In this study, we shows a T-DNA inserted mutants, oscib1-d, which is highly expressed to OsCIB1 by 35S promoter inserts in OsCIB1 promoter region. This gain of function mutant, oscib1-d, has a wide leaf angles and long-slender grain phenotype, in which is similar to brassinosteroid (BR) associated phenotype. In real time PCR analysis, we found that over-expressed OsCIB1 leads to expression of various BR signalling genes, but not leads to BR biosynthesis-related genes. Interestingly, osicb1-d plants showed sensitive phenotype to BR, in which we found a increased cell length in adaxial surface of lamina joint in oscib1-d, resulting in larger angles. Moreover, we found that the cell elongation-associated genes, as Expansins (EXP s) and Xyloglucan endotransglycosylase/hydrolases (XTH s), were significantly increased in oscib1-d. Taken together, these results suggest that OsCIB1 is involved in the BR signaling pathway, and affects not only leaf inclination but also grain shape by regulating cell elongation-related genes. Acknowledgement: This work has supported by "Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ ; B.D. Lee)" Rural Development Administration, Republic of Korea. *Corresponding Author: Byoung-Doo Lee : Tel , bdlee94@snu.ac.kr Nam-Chon Paek : Tel , ncpaek@snu.ac.kr 227

284 PF-14 Byoung-Doo Lee and Nam-Chon Paek * In Arabidopsis thaliana, CONSTANS (CO) plays an essential role in the regulation of photoperiodic flowering under long-day conditions. CO protein is stable only in the afternoon of long days, when it induces the expression of FLOWERING LOCUS T (FT), which promotes flowering. The blue-light photoreceptor FLAVIN-BINDING, KELCH REPEAT, F-BOX1 (FKF1) interacts with CO and stabilizes it by an unknown mechanism. Here we provide genetic and biochemical evidence that FKF1 inhibits CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1) dependent CO degradation. Light-activated FKF1 has no apparent effect on COP1 stability but can interact with and negatively regulate COP1. We show that FKF1 can inhibit COP1 homodimerization. Mutation of the coiled-coil domain in COP1, which prevents dimer formation, impairs COP1 function in coordinating flowering time. Based on these results we propose a model whereby the lightand day length-dependent interaction between FKF1 and COP1 controls CO stability to regulate flowering time. *Corresponding Author: Byoung-Doo Lee: Tel , bdlee94@snu.ac.kr Nam-Chon Paek: Tel , ncpaek@snu.ac.kr PF-15 Jin-Suk Kim 1, Ga-Hyeon Kim 1, Eui-Shik Rha 1, Doh-Won Yun 2, Yong-Eui Choi 3, So-Hyeon Baek 1* Panax ginseng produces triterpene saponins called ginsenosides, which are classified into two groups by the skeleton of aglycones, namely dammarane type and oleanane type. Amoung them, Dammarenediol-II is the basic triterpene skeleton in dammarene-type saponin in Panax ginseng. Dammarenediol-II is a useful candidate both for pharmacologically active triterpenes and as a defense compound in plants. This study was conducted to develop transgenic rice plants that produce Dammarenediol-II by overexpression of Panax ginseng dammarenediol-ii synthase gene (PgDDS). To produce a valuable Dammarenediol-II in rice, recombinant binary vector containing PgDDS gene was introduced into rice plants by Agrobacterium-mediated gene transfer. Through Agrobacterium-mediated transformation of rice calli, 63 transgenic plants were obtained. The results of Southern analysis, transgenic plants contained PgDDS transgene ranging from one to five copies. The compound of Dammarenediol-II, the products of PgDDS, was identified by LC-MS in the T 1 seeds of PgDDS transgenic rice plants. Therefore, PgDDS transgenic plants can be utilized as a source of pharmacologically active medicinal materials, which suggest that availability of Panax ginseng dammarenediol-ii synthase gene could make it possible to engineer ginsenoside biosynthesis to produce ginsenoside aglycones, protopanaxadiol and protopanaxatriol, in transgenic rice plants. *Corresponding Author: Tel , baeksh@scnu.ac.kr 228

285 PF-16 Dong-Keun Lee, Seowon Choi, Jae Sung Shim, Sung Hwan Kim, Hye In Jeong, Chang Mi Kim, Se Nam Lee, and Ju-Kon Kim * Nitrogen (N) is an essential nutrient for plant growth and development. N supply from natural soil is insufficient for crop production and rest of N sources are inevitably supplied by N fertilizer. Although N fertilizer induces great benefits in crop yield, overdose of the fertilizer results in a negative impact on the environment. Improvement of N-use-efficiency (NUE) of crops aims to reduce N fertilizer usage while maintaining crop yield. At first, we developed an N molecular sensor system to monitor N status in a rice plant. We identified two genes for allantoin metabolism, ALLANTOINASE (OsALN) and UREIDE PERMEASE 1 (OsUPS1), to be highly responsive to N status. OsALN was rapidly up-regulated under a low N condition, whereas OsUPS1 was up-regulated under a high N condition. Taking advantage of their nature in response to N status, we generated N sensors as proaln::aln-luc2 and proups1::ups1-luc2 in rice plants. The transgenes mimicked transcriptional regulation of the endogenous OsALN and OsUPS1 genes in response to N status. Importantly, the N sensors showed similar levels of specificity to nitrate and ammonium, inferring the sensing ability of the sensors. Transgenic rice plants with proups1::ups1-luc2 sensor showed strong luminescence activity under a high N condition (> 1 mm N source), whereas transgenic plants with proaln::aln-luc2 sensor showed strong luminescence activity under a low N condition (< 0.1 mm N source). Interestingly, >1 mm N substantially increased internal N levels, indicating the luminescence signals of molecular sensors reflect internal N status in rice. With the N sensor-integrated transgenic rice, we generated an EMS mutant population (10,000 individual lines). We are screening the high NUE rice under low N conditions based on the N sensor system and trying to identify key players in rice N metabolism based on bulk segregant analysis and next generation sequencing techniques. *Corresponding Author: Tel , jukon@snu.ac.kr PF-17 Jin Joo Choi 1*, DongSub Kim 2, Jang Ryol Liu 3, Kyu Hang Kyung A French molecular biologist G.-E. Seralini performed a two-year feeding study of GMO corn and Round-up herbicide in rats and reported increases in tumors and mortality among rats fed GMO corn and the Round-Up, published by Food and Chemical Toxicology in September Numerous independent scientists and regulatory agencies concluded that the study's design was flawed and its findings unsubstantiated. One of the most frequently indicated criticism was that the study used too few rats (10 animals/treatment) to obtain statistically meaningful data, particularly because the strain of rat (Sprague Dawley) used develops tumors at a high rate over its lifetime of about two years. Following widespread criticism by scientists and regulatory agencies over the world, Food and Chemical Toxicology retracted the paper in November 2013 for the reason that its data were inconclusive and its conclusions unreliable. In June 2014 an amended version of the article was republished in Environmental Sciences Europe, a local non-sci journal. Controversial contents of the article and its impact to the public communications of GMO in Korea are to be presented. *Corresponding Author: Tel , frvjj@hanmail.net 229

286 PF-18 Sangyool Lee, Sun Hyun Chang, Taeyoung Um and Geupil Jang * Development of chloroplasts largely affects plant growth and productivity because chloroplasts are responsible for photosynthesis and production of biomaterials such as lipids, amino acids and hormones. Transcription of chloroplast genes is a key step determining the development of chloroplasts, and plastid-encoded RNA polymerase (PEP) is a major RNA polymerase governing the transcription of chloroplast genes. PEP regulates transcription of chloroplast genes as a complex involving interaction with PEP-associated proteins (PAPs), suggesting that PAP proteins are essential regulators controlling the activity of PEP and the transcription of chloroplast genes. We attempted to identify 12 PAPs genes in rice whose expressions are suppressed in the rice grown in darkness. To identify key PAP genes in rice, it was attempted to generate CRISPR/Cas9-mediated rice mutants of the 12 OsPAP genes. CRISPR/Cas9 system can generate target-specific mutagenesis with the activity of guide RNA. Total 24 guide RNAs were designed for 12 OsPAPs mutagenesis (2 independent guide RNAs X 12 OsPAPs), and recombined into prgeb31, a CRISPR/Cas9 binary plasmid. These recombinant DNAs were introduced into rice by rice callus transformation using Agrobacterium tumefaciens LBA4404. Additionally, it was also attempted to isolate full-length cdna of OsPAPs for the generation of OsPAP-overexpressing transgenic rice. Further analysis of CRISPR/Cas9-mediated ospap mutants and OsPAP-overexpressing transgenic rice will expand our understanding of PAP-mediated chloroplast biogenesis in rice. *Corresponding Author: Tel , yk3@snu.ac.kr PF-19 Hyun Young Sing, Chang Kil Kim * Virus-induced gene silencing (VIGS) is widely used for functional analysis of genes in plants. Due to its variation in effectiveness among the plant species, VIGS system was normally optimized using phytoene desaturase (PDS) gene as a visible indicator. In this study, we optimized efficient VIGS system for petunia using PDS gene isolated from pepper (Capsicum annuum) as a visible indicator. Application of the CaPDS gene effectively induce gene silencing in petunia cv. Mirage Rose, cv. Mirage Pink, and cv. Picobella Blue. However, silencing effectiveness was observed to be limited by genotypes and inoculation methods because apical meristem application was found to be an appropriate method, while the highest silencing effectiveness was found in cv. Picobella Blue among the cultivars. In addition, it was found that higher silencing effectiveness was associated with higher degradation of the endogenous PhPDS mrna. Moreover, in term of plant age and temperatures, 3-week-old pants grown at 20 C day/ 18 C night showed high silencing effectiveness for all cultivars. Taken together, infection of apical meristem of 3-week-old plants grown at 20 C day/ 18 C with ptrv2-capds exhibited the highest silencing effectiveness for all cultivars. Therefore, this study indicated usage of CaPDS for VIGS experiment in petunia and involvement of the different factors in the mechanism influencing silencing effectiveness in petunia, and these results will be helpful to enable high-throughput functional analysis of genes associated with commercially important traits in petunia. *Corresponding Author: Tel , ckkim@knu.ac.kr 230

287 PF-20 고세리 *, 장지영, 이주영, 최규환 산업화및기후변화로점차악화되고있는농업환경에서안정적이고지속적으로식량자원확보는국가안보와직결되는문제로인식되고있다. 특히, 미국, 중국등은기후변화대응환경스트레스내성 / 다수성생명공학작물개발연구를전략적으로진행하고있으며, 이와같은생명공학작물종자가세계종자시장에서차지하는비율도점차높아지고있다. 본연구는농업생명공학연구단의생명공학작물개발기술관련산업재산권및육성중인생명공학작물이벤트성과들을종합분석하여, 세계종자시장진입및미래농업환경에대응할수있는우수생명공학작물개발기술을발굴하고, 국내외다양한농생명바이오산업분야로연구개발성과물들이활용될수있는방안을강구하고자한다. 차세대바이오그린 21 사업 1-2 단계선행연구성과로출원된 259 건의산업재산권중국내등록유지특허 109 건에대해 SMART3 특허등급평가로권리성, 기술성, 활용성분야를분석하고총점 BBB 등급이상의특허 55 건을실용화유망우수기술로 1 차선발하였다. 해당선발기술들은기후변화대응농업형질개량을위한분자육종소재개발연구기술 37 건, 형질도입 / 조직배양기술 7 건, 기능성물질생산시스템 7 건이었고, 기능성소재응용기술과검출 / 진단기술은 2 건으로나타났다. 특히생명공학기술로육성한식량작물의재배및활용에관한규제심화에따른연구개발이제한적인현실을감안하여, 생명공학작물의활용분야를국내의 약학품, 화장품, 건강기능식품산업등으로의재설정하고, 고기능성산업용원료소재생산시스템으로서의생명공학식물개발연구성과물을활용하여가치증대하는방안을모색하고, 최신특허동향및산업동향조사기반전략적 IP 포트폴리오및기술패키징을수행할예정이다. * 교신저자 : Tel , skohtn@naver.com PF-21 장안철 1*, 이범규 2, 곽영신 1, 김지영 우리농업의경쟁력확보를위해서는식량안보확보차원의식량생산뿐만아니라부가가치창출과경쟁력제고를위한종자산업등농업생명공학을육성해야한다. 이를위해서는국민들에게농업생명공학에대한객관적이고투명한정보제공과국민들의알권리향상, 지속적인소통프로그램운영을통한농업생명공학에대한이해증진과새로운기술의수용성제고노력을통해국가발전을위한사회적비용을절감해야한다. 최근국내미승인 GMO 작물발견등농업생명공학관련안전성에대한찬반논쟁이심화되고, 국민의안전성에대한우려와안전관리에대한요구도증가하였다. GMO 작물발견지에대한민관협력안전관리등으로국민과의소통기회가이전보다확대되어상호소통을추진한결과농업생명공학에대한상호입장에대한이해부족과이를위한과학적정보기반소통의필요성을인식하였고또한농업생명공학에대한정보공개및서비스제공에대한국민요청이증가하고있다. 이를위해서는농업생명공학에대한올바른과학적정보를체계적으로제공할수있는온라인포털정보시스템의강화와이를통한지속적과학기반정보제공노력이강화되어야한다. 국내몇몇사이트에서 GMO 관련다양한정보를제공하고있으나농업생명공학포털정보시스템에서는단편적정보를포함해보다다양한관점과시각에서과학적이해는물론 GMO 안전성과사회적이슈에대한사회적소통을위한콘텐츠개발을강화하고서비스하는것이더욱중요하다. 본연구에서는대국민소통을목표로다양한과학적, 사회적정보서비스콘텐츠를적극개발제공할수있도록농림축산용 GMO 정보시스템을강화하고자한다. 현재국내외농업생명공학관련정보제공사이트의정보제공메뉴와정보내용등구조분석을추진중이며향후정보제공사이트의구조와정보제공내용개선을집중적으로추진할계획이다. * 교신저자 : Tel , abychan@korea.kr 231

288 PF-22 Young Koung Lee 1,3*, Yinping Jiao 1,2, Zhanguo Xin 2 and Doreen Ware 1, Inflorescence architecture mainly contributes to final grain yield and grain number per panicle (GNP) is a major determinant of grain yield in cereals. Sorghum [Sorghum bicolor (L.) Moench] inflorescence is basically composed of one fertile sessile spikelet (SS) and two infertile pedicellate spikelets (PS). To identify regulatory factors involved in the inflorescence architecture, we screened an EMS mutagenesis population from the pedigreed sorghum mutant library. We found inflorescent architecture mutants, named as multiseeded mutants, msd1,2,3,4, with gained fertile ability in PS and also an increased number of floral branches. In natural sorghum populations, it is not common that are fertile. A detailed dissection of developmental stages of wild type and msd1 mutant described that the PS in wild type do not have floral organs, including ovary, stigma, filament and anther, while the msd1 mutants generate intact floral organ in the sessile spikelet. We found MSD1 encoded a TCP (Teosinte branched/cycloidea/pcf) transcription factor using bulk segregant analysis (BSA) of F2 population, and was a strongly enriched expression during inflorescence developmental stages. We proposed that MSD1 functions to suppress floral organ maintenance at PS during inflorescence development in Sorghum. To explore the regulatory network associated with PS fertility, whole genome expression profiling was performed at 4 different developmental stages in 6 various tissue types between wild type and msd1. Whole-genome expression profiling reveals that jasmonic acid (JA) biosynthetic enzymes are transiently activated in pedicellate spikelets. Young msd1 panicles have 50% less JA than wild-type (WT) panicles, and application of exogenous JA can rescue the msd1 phenotype. Our results reveal a new mechanism for increasing GNP, with the potential to boost grain yield, and provide insight into the regulation of plant inflorescence architecture and development *First Author: Tel , leeyk@cshl.edu PF-23 Su Hyun Park *, Seunghoon Baek, Ara Cho, Jeong-Hwan Mun, Sang-Bong Choi Long non-coding RNAs (lncrnas) play a vital role in a wide variety of gene regulatory networks related with entire developmental processes and environmental cues. So far, identification and functional analysis of lncrnas have been limited to several model plant species. Although Brassica species are important vegetable crops, their lncrnas are not well-predicted thereby only less than 3200 lncrnas are reported mainly focusing B. napus. To explore lncrnas in Raphanus sativus, we analyzed RNA-Seq data generated from distinct 18 tissues and developmenta stages that encompass 152 Gb and 1.7x10 9 reads. Of the total 17,448 lncrna candidates, 12,466 were located in intergenic regions, and 5,022 lncrnas were in genic. The 11,148 lncrnas were predicted as antisense while 6,340 were sense RNAs, and mapped on the radish genome (n=9, Mbp). To identify tissue-specific patterns, we compared the expression levels of lncras derived from 5-week old seedling, 10-week old leaf and root, petal, anther, and pistil. Total 3,230 differentially expressed lncrnas were obtained. Further, differentially expressed lncrnas that are related with vernalization were analyzed, and thereby 309 lncrnas were neo-synthesized and 77 were repressed at the end of 10-week cold treatment. Our predicted lncrnas will be verified in their expression and functionally analyzed. *Corresponding Author: Tel , shattersun@naver.com 232

289 PF-24 윤도원 1*, 오성덕 1, 손수인 1, 장안철 1, 백소현 2, 최용의 사포닌은식물의이차대사산물에서다양하고풍부한물질중의하나로, 인삼, 콩, 도라지, 미나리, 마늘, 양파, 영지버섯, 은행, 칡등에풍부하며, 그중인삼은생리활성이가장높은진세노사이드사포닌을함유하고있다. 인삼의주요약리성분인사포닌합성유전자를벼에형질전환하여사포닌합성을유도하였다. 사포닌합성벼의엘리트라인을선발하기위해약 60 라인을포장에전개하였다. 모든라인의종자를 50 립씩파종하여 2 주후선발마커인 bar 유전자의삽입을확인하기위해제초제 ( 바스타 ) 를처리한후생존개체를 12 주씩정식하였다. 이개체들에대한유전자삽입여부, 삽입 copy 수, 삽입위치등을분석할예정이다. 엘리트라인중에서모본과농업형질이유사한계통을선발하여후대고정할예정이다. * 교신저자 : Tel , dwyun@korea.kr PF-25 윤도원 1*, 오성덕 1, 손수인 1, 장안철 1, 이효연 2, 서석철 2, 선현진 GM 작물을개발또는수입하여환경에방출하는등의재배또는상업화를목적으로하는경우에는반드시 GM 작물의환경안정성평가항목에따른과학적인실험결과를토대로안전성평가심사를거쳐야만한다.. 또한, 전세계적으로 GM 작물의개발대상형질이점점다양화되고, 재배면적이급속하게증가하고있으므로, 우리나라도이러한 GM 작물개발과생산에대비해야한다. 따라서작물의특성별로 GM 작물안전성을평가할수있는위해성평가모델시스템을개발하여국내에서 GM 작물을개발하는연구자들이활용할수있도록기반을조성하는것이매우중요하다. 제초제저항성잔디의개발되어실용화전단계에이르렀는데, GM 잔디의전주와제주지역의생육상의비교평가를통해지역적응성시험을실시하였다. 또한 GM 잔디재배포장주변의환경모니터링을실시하여비의도적환경방출의가능성을검토하였다. * 교신저자 : Tel , dwyun@korea.kr 233

290 PF-26 Yun Young Kim and Jeong Sheop Shin * Alfalfa has widely been cultivated to be used as forage for livestock due to its high nutrient content. However, the digestibility and utilization of alfalfa in livestock industry is hampered by its lignin content. Lignin is a cell wall component along with cellulose and hemicellulose constituents that accumulates in the plant tissues, particularly in the stem. While a certain amount of lignin is essential for healthy plants to be stand upright, lignin is an indigestible component of plants and reduces the fiber digestibility of forages in the rumen of livestock. Therefore, forage producers and commodity purchasers desire alfalfa with lower lignin levels but without loss of nutritional components such as protein and fiber (also called as high-quality alfalfa ). In this work, we intend to develop biotechnology-derived high-quality alfalfa which has reduced lignin and increased biomass using useful genes including CBSX2 (Cystathionine-β-synthase Domain-Containing Protein 2) and novel promoters which were screened from our previous studies with Arabidopsis thaliana. Through intensive phenotype analysis, we demonstrated that CBSX2-overexpressed (CBSX2-Ox) Arabidopsis plants showed reduced lignin deposition in the stem as well as delayed senescence and abscissions that led elevated biomass production when compared to wild-type plants at the same growth stage. These results strongly indicate that the CBSX2 gene is a useful source to substantially improve quality and yield traits of alfalfa. Reproducibility of these phenotypes is going to be re-evaluated with CBSX2-Ox alfalfa plants that are currently under construction. *Corresponding Author: Tel , jsshin@korea.ac.kr PF-27 Seon-Woo Oh 1*, So-Young Lee 1, Hwi-Young Park 1, Soo-Yun Park 1, Sang-Gu Lee 1, Seong-Kon Lee 1, Young-Soo Chung 2, Hyun-Suk Cho This study was to evaluate the nutritional profiles of 14 kinds of soybeans cultivated in the three locations which were Daegu, Suwon, and Jeonju of south Korea. All of these soybeans were cultivated by standard and conventional agricultural practice. Commercial varieties of soybeans were analyzed to compare the nutritional profiles according to the different environmental conditions. The ph of soil, annual rainfall, and weather temperature were investigated for the environment differences. The micronutrients of vitamine, minerals and fatty acids were investigated for the environmental effects. The contents of minerals were not different among three locations but the contents of amino acid and lipid were different significantly (p<0.05) among three locations. Micronutrients were affected more by the environment conditions such as cultivated locations than kinds of varieties. The fatty acid profile showed that linoleic acid, (10.2~12.5% of total) was the most abundant fatty acids followed oleic acid, palmitic acid, linolenic acid and stearic acid. Analyses of mineral content indicated that the most abundant mineral was potassium, followed by magnesium, calcium, iron, zinc, sodium and manganese. These data could be used in the safety assessment of new plants developed by the biotechnology by comparing the substantial equivalence. *Corresponding Author: Tel , ohsw0507@korea.kr 234

291 PF-28 Gagan Kumar Panigrahi, Ki Hun Shin, Annapurna Sahoo, Kyung Man Kim, Sang Hyon Kim * Nonsense mediated mrna decay is an essential mrna control process in eukaryotes that eliminates potentially harmful transcripts containing premature termination codons. Although splicing errors may generate aberrant transcripts carrying upstream ORFs, intron(s) in the 3 UTR or long 3 UTR, the natural transcripts may also become NMD targets by possessing the above NMD-triggering features. A subset of Arabidopsis Resistance genes transcripts are reportedly stabilised during bacterial infection, which suggests decrease of the NMD efficiency by this event. However, whether or not the individual R transcripts are the NMD substrates, and molecular details on release of NMD by bacterial challenge remain at large. We showed that 81.2% and 65.1% of fully spliced natural TIR-NBS-LRR (TNL) and CC-NBS-LRR (CNL) transcripts, respectively, retain signatures of NMD regulation. Recognition of bacteria initiates the destruction of UPF1, UPF2 and UPF3 within 30 minutes post-infection via the independent ubiquitination of UPF1 and UPF3 and the 26S proteasome pathway, and subsequently, NMD-sensitive TNL and CNL transcript levels increase. Induction of UPF1/UPF3 ubiquitinations was delayed specifically in mpk3 or mpk6. Our findings therefore demonstrate that NMD is the control tower through which pattern recognition receptors can fine-tune NLR transcript levels to reduce fitness costs and achieve effective immunity. *Corresponding Author: Tel , kim6189@mju.ac.kr PF-29 박정호, 박지영, 엄민식, 김혜진, 김창기 * 콩은단백질함유량이높기때문에단백질화장품소재생산에적합한작물이다. 본연구에사용된형질전환콩의경우기존에이용되어온대장균발현방법에비하여화장품소재단백질생산단가의 90% 이상절감이가능하며콩재배농민들에게기존일반콩수매가보다 6 배이상의가격으로수매가가능할것으로예상된다. 본연구에서는화장품소재단백질을생산하는형질전환콩 2 종류의인체위해성평가와환경위해성평가중잡초화가능성평가를수행하였다. 형질전환콩의인체위해성평가를위해서대장균에서대체생산을위한도입단백질의대량정제법을개발하였다. 또한대장균에서생산된대체단백질과형질전환콩의도입단백질과의동질성검정을위하여 SDS-PAGE 분석, Western blot 분석및 N- 말단분석을진행하였다. 형질전환콩의환경위해성평가중잡초화가능성검정을위하여토양에매몰된형질전환콩종자의활력과잡초와경합하는환경에서지속성을분석하였다. 본연구를통하여비임상단회투여독성검정을위한시료생산법을개발하였고형질전환콩에도입된단백질의정제를위하여항체를제작하고항체컬럼을제작하는데성공하였으며이를이용하여 10 개 N- 말단아미노산서열이대장균에서발현된단백질서열과일치하는것을확인하였다. 토양에매몰된형질전환콩와모품종콩종자모두월동이가능하지만 6 개월이내에활력을잃었다. 이번연구의결과는새롭게개발되는고부가가치형질전환작물의위해성평가방법개발에기여할것으로사료된다. * 교신저자 : Tel , cgkim@kribb.re.kr 235

292 PF-30 신윤희 1*, 우은택 2, 박영두 점박이응애 (Tetranychus urticae) 는약 3,800 개의넓은기주작물범위를가지고있는잡식성해충이며, 한국에서중요한가치를지닌채소중하나인배추도점박이응애에의해피해를입는다. 또한살응애제에저항성을갖는점박이응애개체의증가로이를방제할다른방안의필요성이대두됨에따라본연구는식물매개 RNA interference(rnai) 기법을적용하기위해수행되었다. 배추의형질전환을위한 RNAi vector 는점박이응애에서유래한상보적인 COPB2 유전자단편과선발마커로사용된 bar 유전자를포함하였다. Agrobacterium 을이용한형질전환이수행되었고 DNA 및 RNA 수준의분석을통해생명공학배추를선발하였다. 점박이응애 50 마리를접종하여생물검정을수행한결과, 생명공학배추의잎을흡즙한점박이응애는낮은생존율을보이다접종 10 일후에는모두사멸되는것을보아 RNAi 기법이적절히작동하여생명공학배추가점박이응애에저항성을보이는것으로확인되었다. 또한 T-DNA 내부 PCR 분석을통해도입된 T-DNA 의내부변형없이안정적으로도입되었음을확인하였으며 T-DNA 삽입지역의분석을통해도입된 T-DNA 가 intergenic 지역에삽입된것을확인하였다. 이후선발된생명공학배추는뇌수분을이용한세대진전을하여선발과고정단계를진행하고있다. 본연구결과 RNAi 기법을이용한점박이응애저항성생명공학배추의개발이가능함을입증하였다. * 교신저자 : Tel , yunhee94@naver.com PF-31 Seungsu Han 1*, Ji-Young Lee 2, Yeongmok Lee 1, Jiseok Beak 1, Jaeeun Jung 1, Tae-Houn Kim 2, and Sangho Lee The abscisic acid (ABA) signaling pathway is based on protein-protein interaction between clade A type 2C protein phosphatases (PP2CAs), ABA receptor (PYL/RCAR) and kinase protein (SnRKs) in plants. In stress, ABA trigger for PP2Cs to release stress/aba-activated protein kinases by binding to ABA-bound receptors for activation. Thus, the interaction of PP2CA with PYL/RCAR is a signaling trigger to respond biotic/abiotic stress. Although the wedging tryptophan in PP2Cs is critical in the interaction with PYL/RCARs in Arabidopsis and rice, it remains elusive as to how other interface regions are involved in the interaction. Previously, we reported the identification of a conserved region on PP2Cs, termed the VxGΦ L motif, which modulates the interaction with PYL/RCARs through its second and fourth residues. In this study, the effects of the second and fourth residues on the interaction of OsPP2C50 with several OsPYL/RCAR proteins are investigated by systematic mutagenesis. ABA response strength can be finely tuned with the alteration of hydrophobic environments of the motif to tiny, modest and bulky residues. The fourth residue of the VxGΦ L motif seems to be a major determinant of the interaction between OsPP2C50 and OsPYL/RCAR3 by X-ray crystallography, HDX-MS, bio-layer interferometry (BLI) and phosphatase inhibition assay. Modulation of ABA signaling by mutations in the VxGΦ L motif is demonstrated using rice protoplast system and transgenic Arabidopsis plants. Taken together, the VxGΦ L motif of PP2Cs appears to modulate the affinity of PP2Cs with PYL/RCARs and thus is likely to alter the ABA signaling, leading to the differential sensitivity to ABA in planta. *Corresponding Author: Tel , hsspoket@hanmail.net 236

293 PF-32 문성윤 1, 강세라 1, 김영군 1, 은창욱 1, 손영진 1, 오성덕 2, 박수윤 2, 장안철 2, 서상재 1* 1 2 형질전환대두 ( 비타민 A 강화콩등 ) 의환경방출과비의도적환경방출에의한야생콩과의교잡종이곤충상에미치는영향평가방법개발한다. 생태계에서식하는곤충군집은구성하는종마다생물학적및생태학적특성이다르기때문에적절한모니터링방법을사용하여야군집및개체군의변동을해석할수있으며, 조사방법에따른모니터링방법간상호효율성평가한다. 또한, 형질전환대두 ( 비타민 A 강화콩등 ) 재배에따른곤충상의발생양상변화및잠재해충의발생가능성등생물환경안전성평가를위한형질전환대두및야생콩과의교잡종에서해충과천적등곤충군집상에대한영향을분석하기위하여, 포장에발생하는주요곤충과거미류등의절지동물의계절발생과먹이그물을고려하여절지동물군집구조, 생물다양성, 발생밀도및생태학적기능군의구조를분석한다. * 교신저자 : Tel , sjsuh@knu.ac.kr PF-33 오성덕 1*, 장안철 1, 서상재 2, 윤도원 1, 박수윤 GM 콩은전체 GM 작물재배중 50%(9,140 만 ha) 를차지하여가장많이재배되는작물이며, 특히우리나라는야생콩의지리적원산지로서자연생태계확산과이에대한안전관리문제를야기할수있다. GM 콩의환경위해성평가와안전관리를위해서 GM 콩로부터야생콩으로수직유전자이동가능성, 교잡콩의잡초화 / 침입가능성, 종자의특성, 타감작용평가기술을연구개발할필요성이있다. 제초제저항성유전자를포함한기능성물질생산 GM 콩 ( 비타민 A 강화콩등 ) 을이용하여 LMO 격리포장에서개화기일치등자연교잡조건을확립하고야생콩으로의유전자이동을통한교잡종을개발하여교잡종의월동성, 휴면성, 생식생육특성, 경합력및침입성등잡초화가능성항목조사설정및조사한다. 복수년차이상의기간동안교잡종의생식, 생장특성을조사하기위해장기영향평가포장을조성하여생태, 생식등의변화를대조군과비교하여교잡종의잡초화가능성평가및환경모니터링분석방법을개발할예정이다. * 교신저자 : Tel , ohbaboh@korea.kr 237

294 PF-34 오성덕 1*, 박정호 2, 김재광 3, 장안철 1, 손수인 1, 윤도원 1, 박수윤 GM 작물은 2016 년기준, 26 개국 백만헥타르에서재배중이며, 최초상업화대비 100 배이상성장하였으며, 세계종자시장의 35% 를차지하고있다. 현재전세계재배되는콩중 82% 가형질전환콩이며, 전체재배된 GM 작물중 50% 에달하는가장많은비중을차지하고있어경제적 상업적가치가높은작물이다. 현재국내에서상업적인목적으로재배 판매되고있는형질전환콩은없으나, 형질전환콩의환경위해성평가와안전관리를위해서주변환경에미치는영향에대한조사, 분석하고농업환경생물종위행성평가기술을연구개발할필요성이있다. 이를위해화장품소재단백질생산용형질전환콩에대한농업환경생물종선정및시료생산하고농업환경생물종평가용형질전환콩과비형질전환콩시료생산하고농업환경생물종위해성평가방법및평가기준설정한다. TRX/EGF 단백질생산형질전환콩에대한농업환경생물종의영향평가를수행하고비의도적인환경방출에의한형질전환콩도입유전자발현도분석할예정이다. 또한, 화장품소재용 TRX/EGF 단백질생산형질전환콩에대한농업환경생물종의영향평가표준화및가이드제시할것이다. * 교신저자 : Tel , ohbaboh@korea.kr PF-35 Joohee Choi 1,2*, Pil Joong Chung 1,2, Jooyeon Bae 1, Jung Suk Kim 1, Jae Suk Jan 1, Ju-Kon Kim 1* 1 2 Abiotic stresses are major constraints of agricultural productivity. Drought, the most serious stress, and its negative impacts are likely to increase worldwide. Recent studies have shown that abiotic stresses induce aberrant expression of many noncoding RNAs, including mirnas, thus suggesting that mirnas may be promising targets for genetically improved crop tolerance to abiotic stresses. In general, abiotic stress induces mirnas to downregulate their target mrnas, and their downregulation leads to accumulation and activation of positive regulators. This implies that mirnas do not control directly plant growth and development but control indirectly plant development by mediating a mirna-target gene network. Therefore, it is evident that endogenous mirnas have been shown to work as developmental switches and to regulate drought-responsive genes under drought stress. Previously, we identified the rice noncoding RNAs (66 mirnas and 98 lncrnas), whose expressions were highly regulated by drought conditions, and whose transcript levels were negatively correlated with the putative target genes. For a further investigation of the biological functions of each mirna, we generated 12 mirna overexpressing and knockout lines using constitutive GOS2 promoter and CRISPR/Cas9, respectively. During cultivation, we found several phenotypes in the overexpression lines, including premature leaf senescence, increased number of tillers and grain yield along with the drought tolerance phenotype. The use of mirna-overexpressing and knockouts and their targets will be a promising technique for determining the native functions of individual mirnas in response to drought stresses. The identification of the specific positions of mirnas underlying their regulatory networks represents a convincing research area to pursue in the future. *Corresponding Author: Tel , solivefree@snu.ac.kr 238

295 PF-36 Youn Shic Kim 4*, So-Yoon Seong 4, Jong Hoon Kim 1, Sue Yeon Lee 2, Woo Jin Kim 1, Jae Young Choi 2, Seung Tae Kim 3, Seok Hee Lee 1, Ying Fang 1, Dong Hwan Park 1, Min Gu Park 1, Ra Mi Woo 1, Bo Ram Lee 1, Ju-Kon Kim 4, Yeon Ho Je The transgenic rice expressing cry1ac gene, which is linked to the rice rbcs promoter and its transit peptide sequence (tp), was highly resistant against all instars of Cnaphalocrocis medinalis (Guenetée) (Lepidoptera: Crambidae). In this study, we evaluated the larval mortality, behavior change, and field occurrence of three main rice pests, C. medinalis, Naranga aenescens (Moore) (Lepidoptera: Noctuidae), and Parnara guttata (Bremer & Grey) (Lepidoptera: Hesperiidae) in T4 generations of three Bt rice events (rbcs3:cry1ac; , and ) and non-bt rice. All of the three Bt rice events were resistant to C. medinalis which showed significantly higher mortality for all instars compared to non-bt rice. The resistance of Bt rice events against the larvae decreased gradually as the larvae developed. However, the survived larvae which ingested Bt rice events died eventually without further development. The resistance of three Bt rice events was investigated in the pot test, which was conducted with 3rd instars of C. medinalis, N. aenescens, and P. guttata, showed mortalities of over 70%. In behavioral assay, C. medinalis fed on the Bt rice events showed feeding avoidance and less leaf rolling behavior compared to that of the larvae fed on non-bt rice. A 2-yr field survey conducted with larvae of C. medinalis and P. guttata also showed that the three Bt rice events significantly had lower damaged on leaves compared to that of non-bt rice. Overall, the three Bt rice events were highly resistant to the larvae of lepidopteran target rice pests. *Corresponding Author: Tel , younshic@snu.ac.kr PF-37 정기홍 *, 문선옥, 김유진, 유요한, Anil Kumar N.C, 김효용, 홍우종, 배도영, 안혜련 차세대바이오그린 21 사업과포스트게놈다부처유전체사업을통해농생명빅데이터생산및관련자료축적이급속히증가했지만이의활용을위한연구는여전히제한적이다. 또한, 현재까지농업형질이개선된우수한형질전환식물체가여럿제작되었음에도불구하고유전자변형생물체에대한오해및우려가높아이를실용화하기란매우어려운실정이다. 따라서, 이를극복하기위해사람이인위적으로변형한형질전환식물체가아닌자연적으로변이가일어난벼품종을활용하여유용작물을발굴할필요가있다. 우리는차세대염기서열해독기술을활용하여 483 국내재배벼및핵심계통에대한전장염기서열해독자료를기반으로개별벼유전자단위 (Locus ID) 로 reference 벼품종인 Nipponbare 에대해 insertion/deletion (indel) 형성여부를확인할수있는데이터베이스를공주대학교연구팀의도움으로구축하였다. 이를토대로기존에보고된논문중가뭄스트레스에저항성을가지는유전자 5 개를선발한후, 5 개유전자모두에서 indel 형성이일어난품종 5 개 (Cheongdo-donggok-4, Chungdo Hwayang 12, Wase Gingbouzu, AI-CHIAO-HONG, GUAN-YIN-TSAN), indel 형성이하나도일어나지않은품종 5 개 (Pyeongbuk 3, Golyeong-2, Orido, Jejubukjeju , Jejubukjeju ) 를확보하였다. 그다음정말로 indel 이형성또는형성되지않았는지 sequencing 을통해다시한번재확인할예정이다. 이는염분스트레스에저항성을가지는품종을선발하는과정에서도동일하게적용될것이다. 현재공동연구를통해서관련품종이전개되어있으며, 돌연변이가확인된품종에대해서수확후가뭄및염분스트레스실험을수행할예정이다. 우리는이과제를통해빅데이터를활용한유용품종을탐색및확보하고, 이를실용화할수있는토대를만들고자한다. * 교신저자 : Tel , khjung2010@khu.ac.kr 239

296 PF-38 우은택 1*, 장창순 1, 박영두 배추의농업애로사항해결을위한분자육종방법에는배추자체의형질개선을이용하여재배및육종을용의하게하는방법과생물학적및비생물학적스트레스에대한저항성을부여하는방법이있다. 배추에서의자가불화합성은 1 대잡종종자를유지하기위한모본유지시뇌수분, CO 2 처리등을사용하고있어인건비및탄소배출등의문제가있다. 또한배추좀나방의경우식해량은적으나살충제에대한감수성이낮아살충효과가적어큰피해를주고있으며, 흡즙성해충인응애또한잎의조직을파괴하여생육에영향을주게된다. 그러므로전통육종및분자육종의유기적조화를통한신품종개발은이러한육종효율증진및배추병해충피해억제에도움이될것이다. 이에따라, 본연구에서는분자생물학적방법으로선발된유용생명공학배추를이용하여, 주요목표형질과함께농업형질의실질적동등성을확인하고선발을통하여유용계통으로의고정여부를판단하였다. 각형질들은국립종자원에서공시한작물별특성조사요령에맞추어식물체의초장, 바깥잎의크기및모양등과같은총 33 가지의특성들을조사하였다. 이후배추형질전환에사용된대조군인 inbred line CT001 과생명공학배추를비교분석하여유용개체를선발및계통화여부를결정하였다. 분석결과원예형질의실질적동등성은확인되었으며, 해충에대한섭식피해가유의적으로적은계통들이확인되었다. 이와같은연구결과는생명공학배추의형질분석및고정연구에기반이될것이다. * 교신저자 : etwoo1@hanmail.net PF-39 이강섭 * 국내에서의 GM 작물개발은유전자의지적재산권결여, 우량형질전환이벤트부족, 소비자의사회문화적수준을고려하지않은 GM 작물개발등의문제점으로인하여아직까지실용화된예가없다. 따라서 GM 작물의개발초기부터, 품종화까지모든단계에적용되는체계적인 GM 작물개발프로토콜과기술이필요하다. 본연구에서는유전체연구를통하여유전자기능을검정한후보들을활용하여환경저항성관련유용유전자를선발하였으며, 국내고유로개발된벡터를이용하여 35S:BrTSR15, 35S:BrTSR53, 35S:ArCspA, 35S:PsGPD 벡터로재구성하엿다. Agrobactrium(LBA4404) 을이용하여총 383 개의독립된형질전환벼식물체를제작하였으며, TaqMan real time-pcr 방법으로 copy 수를분석하여, 35S:BrTSR15 는 29 계통, 35S:BrTSR53 는 38 계통, 35S:ArCspA 는 52 계통, 35S:PsGPD 는 84 계통의 one copy T-DNA 삽입식물체를확보하였다. TaqMan copy number assay 방법을이용하여확보된 1 copy 삽입식물체를 FSTs 분석방법을이용하여 T-DNA 삽입위치를분석하여 intergenic 식물체를선별하였다. 1 copy/intergenic T-DNA 삽입형질전환벼식물체를세대진전하여 T1 세대에서 copy number 분석법과배지에서항생제선별방법을이용하여 homozygosity 계통을 27 계통선발하였다. 형질전환체의발현유무를확인하기위해 T0 의재분화식물체를 RT-PCR 을수행하였다. 대부분의식물체들이정상적으로발현된것을확인하였다. 이벤트개발을위해확보된식물체는계속적으로세대진전을하며포장에서의표현형검정을할예정이며또한각유전자의기능검정을위해분자생물학 생리학적분석을수행중에있다. * 교신저자 : Tel , kangslee@korea.kr 240

297 PF-40 * 朴钟泽 GM 작물개발에활용할플랫폼을구축하기위하여사업단에서추천하는국내개발유용유전자를분양받아형질전환시스템을확립하고, 이들유전자를조기에고정하여이벤트를개발하는것을목표로한다. 또한개발된이벤트를육종프로그램을활용하여각지역에적합한품종에의도입을목적으로최적의육종프로그램을개발하려한다. 또한국내특허보유유전자및운반체를중국에제공하여, 중국지역에적합한품종에도입하여세계시장진출의교두보로활용하려하고있으며, 고정품종뿐아니라이벤트를인디카계열의웅성불임계통에교배를통해유전자를이전하여 1 대잡종품종을개발하는것을목표로한다. 내재해성및내충성, 내병성을검정하기에적합한지역을선정하여후보유전자의기능을검정하고이들우수계통을이벤트화하고중국내각지역에적합한 GM 품종을개발하고이들품종을동남아지역까지확대해서보급하려는과제이다. 금년도중국해남도세대촉진시험단지에서 68 교배조합을작성하였으며, Bt 벼품종계통 200 개를세대촉진하였다. PGMS 불임계통 16 개와품종비교시험계통 8 개를종자증식완료하였다. 작년 Bt 잡종강세시험결과를토대로그중우수한 12 조합을대량체종하여현재품종비교시험을통한생산성검정시험을진행하고있는중이다. Bt134 GM 계통을중간시험단계를완료하고현재중국농업부에환경방출시험허가를받아환경방출시험을실시하고있다. * 교신저자 : Tel , zzpaio@hotmai.com PF-41 Wen-Xing Hu 1, Jung-Kyu Kim 1, Hye-Kyoung Ju 1, Hanhong Bae 2, Leslie L. Domier 3, Hyoun-Sub Lim 1* Several CRISPR-Cas9 orthologues have been used for genome editing in plant or animal systems, etc. Recently, a small Cas9 orthologue derived from Campylobacter jejuni (CjCas9) has been shown to be functional for efficient genome editing in vivo in animals. Here we used a potexvirus viral vector to efficiently express CjCas9 proteins systemically in Nicotiana benthamiana, with confirmation of expression in the newly-formed upper leaves demonstrated by RT-PCR. The single-guide RNA (sgrna) required for activity is packaged in the viral vector multiple cloning site, flanked by hammerhead ribozyme sequences at both sides, such that the sgrna is able to precise self-cleavage at these specific site to release the mature sgrna sequence. Furthermore, a Theophylline-dependent hammerhead ribozyme switch is also used to control the release of the sgrna. Our work is targeted to production of genome edited seed from the plant viral-cas9 infected crop, which is expected to be a more convenient, cost-effective, and high-efficiency genome editing method. *Corresponding Author: Tel , hyounlim@cnu.ac.kr 241

298 PF-42 Yun-Shil Gho 1*, Sunok Moon 1*, Eun-ha Park 1, Ki-Hong Jung 1 1 This study has a purpose to identify the root development promoting genes using system biology and advanced rice genetic resources and to confirm the enhancement of nutrient utilization efficiency. To do this, a large number of genes predominantly expressed in the root hair or root of rice were identified and analyzed, and seven genes involved in root hair development and revealed by knockout mutations or overexpression studies were selected. Among them, one of overexpression line enhanced tolerance in response to phosphate starvation, and phosphorus content of the transgenic rice plant were about 50% higher than those of the wild-type plant. Another strategy is the evaluation of root-preferred promoters. To investigate the applicability of root-preferred promoters, transgenic rice plants overexpressing OsPT4 which is involved in phosphate transport were generated by transforming root-preferred promoter::ospt4 and root hair-preferred promoter::ospt4 into Dongjin cultivar. Later, we will test the possibilities for enhanced phosphate use efficiencies compared to those of ubiquitin promoter::ospt4 plant. In addition, we analyzed big data sequence information for 483 rice varieties and checked the genetic variations of known nutrient use efficiency relating genes. To confirm the variations of these genes among rice varieties, we selected 23 varieties and will analyze the variation patterns. After then, we will utilize the related varieties to confirm the possibility of application as a breeding material of discovered good agricultural traits through natural variation. *Corresponding Author: Tel , khjung2010@khu.ac.kr PF-43 Hyung-Keun Ku 1#, Young-Joo Jung 1#, Yeo Jin Lee 1, Byeong Hoon Kim 1, Min Kyoung You 1, Ye Sol Jeong 1, Jae Kwang Kim 2, Sun-Hwa Ha 1* 1 2 As a proof-of-concept, the β -carotene biofortified rice, stpac (stpsy-f 2A-PTp-stCrtI) rice, has been developed via bicistronic expression of rice codon-optimized two synthetic genes encoding phytoene synthase (PSY) and carotene desaturase (CRTI) using ribosomal pausing 2A sequence (F 2A ) derived from mammalian virus. To develop this β -carotene enriched rice events being able to be commercialized, safety of transgenes were evaluated and vector components to minimize patent infringement and improve the efficiency of transgenes expression were reconstituted. By allergeniocity test, any elements in recombinant proteins were not found to possess similarities to known allergens. Through intensive patent search, patent-free vector, endosperm-specific promoters and chloroplast targeting transit peptides (Tp) were found, leading vector replacement from pmj103 into ppzp200 and selection of globulin (GB) promoter from other two endosperm-specific promoters, gluterin-type B and C promoters and PTp/R3Tp from R1Tp, as most efficient components on β -carotene production in rice endosperm. By comparison of ribosomal pausing activity among 2A sequences, T 2A derived from non-mammalian virus were selected to replace F 2A since T 2A induced complete ribosomal pausing compared to F 2A, only inducing ~80% ribosomal pausing. On the basis of these results, we generated GB::stPTAC (GB::stPsy-T 2A -PTp-stCrtI) and GB::stPTARC (GB::stPsy-T 2A -R3Tp-stCrtI) constructs and integrated them into rice genome. Through mass production of rice transformants, 325 stptac and 239 stptarc plants were obtained and 90 and 68 plants were selected as 1 copy-insertion lines by TaqMan-PCR. 45 single T-DNA insertion in stptac plant was confirmed by further flanking PCR with leaf tissue at T 0 generation. Among them, 23 plant lines showed the intergenic gene insertion after confirmation of their flanking loci in either or both ends of right and left border on rice genome. The same analysis regarding flanking PCR and intergenic gene insertion in stptarc plants are being carried out. Collectively, we obtained the final candidates satisfying the eligibility condition as GMO events with 7% efficiency from initial number of rice transgenic plants. #co-first authors: Hyung-Keun Ku and Young-Joo Jung *Corresponding Author: Tel , sunhwa@khu.ac.kr 242

299 PF-44 Ye Sol Jeong 1#, Hyung-Keun Ku 1#, Young-Joo Jung 1, Jae Kwang Kim 2, Sun-Hwa Ha 1* 1 2 In our previous study, the β -carotene biofortified rice, PAC (Psy-F 2A -CrtI) rice, showing golden color phenotypes was developed via a bicistronic expression of two genes encoding synthetic phytoene synthase (PSY) and carotene desaturase (CRTI) involving a ribosomal pausing 2A peptide (F 2A) from foot and mouth disease virus (Ha et al., 2010). In succeeding study, the more β -carotene-accumulated version of PAC rice, stpac (stpsy-f 2A -stcrti) rice were invented by replacing Psy and CrtI gene into codon-optimized synthetic Psy (stpsy) and CrtI (stcrti) (Jeong et al., 2017). To overcome the limit of a mammalian pathogenic viral origin, 2A peptide sequences from Thosea asigna virus (T 2A) and Infectious myonecrosis virus (I 2A) were chosen from non-mammalian viral origins considering the reported in vitro efficiency and examined in planta their efficiency in transgenic rice endosperms. As a result, two recombinant genes of stptac (stpsy-t 2A -stcrti) and stpiac (stpsy-i 2A2 -stcrti) were built to compare their efficiencies for β -carotene production. Both transgenic rice plants expressed golden color and accumulated β -carotene in rice endosperms. Expression analysis of transgenes showed higher expression of proteins in stptac than stpiac line. Interestingly, none of stptac and stpiac lines generated a large form of fusion protein, PSY-2A-CRTI, unlike stpac line, supporting the better efficiency of both T 2A and I 2A2 than F 2A to induce ribosomal pausing. Therewith, by linking two additional synthetic genes encoding β -carotene hydroxylase (BCH) and β -carotene ketolase (BKT), required for zeaxanthin and astaxanthin biosynthesis, into stptac, tri-, tri- and tetra-cistronic expression constructs, stpsy-t 2A -stcrti-i 2A1 -stbch as stptac-iabc, stpsy-t 2A -stcrti-i 2A1 -stbkt as stptac-iabk, and stpsy-t 2A -stcrti-i 2A1 -stbch-i 2A2 -stbkt as stptac-iabc-iabk, were generated, and successfully produced zeaxanthin, astaxanthin and astaxanthin in rice endosperm, respectively. Especially, accumulated astaxanthin in stptac-iabk indicated that BCH is not indispensable and can be omitted for astaxanthin metabolic engineering. Immunoblot analysis exhibited co-expression of PSY and CRTI in stptac-iabc and stptac-iabk lines but only CRTI in stptac-iabc-iabk line. #co-first authors: Ye Sol Jeong and Hyung-Keun Ku *Corresponding Author: Tel , sunhwa@khu.ac.kr PF-45 So Young Park, Dong-Hoon Jeong * Epigenetic regulation has been implicated in the many aspects of plant development and stress responses. The underlying mechanisms of epigenetic regulation include DNA methylation, histone modification, and non-coding RNA-mediated regulation of gene expression. Of these, non-coding small RNAs, including micrornas (mirnas) and small interfering RNAs (sirnas), play a crucial role in negative regulation of gene expression at both transcriptional and posttranscriptional levels. To study the epigenetic role of rice small RNAs, small RNAs were sequenced from drought stress-treated seedlings and panicles as well as from control tissues. Using a static clustering method, we identified sirna loci that matched to a set of nt sirnas that was significantly up- or down-regulated by drought stresses. This result implies that stress-inducible or repressible sirnas may contribute to epigenetic regulation of gene expression. We also examine the possible role of a mirna in epigenetic regulation of environmental stress response. In rice, mir820 has been known to be down-regulated by drought stress. It targets OsDRM2, which is involved in de novo DNA methylation of CG and non-cg sequences in the rice genome through a RNA-dependent DNA methylation mechanism. To explore the function of mir820 during drought stress, transgenic rice plants over-expressing mir820 was generated. The transgenic plants exhibited drought-resistant phenotype compared with wild type plants. In addition, several transposable elements, including RIRE7, CACTA, and Tos17, were up-regulated in these transgenic plants. These results might be due to down-regulation of OsDRM2, which is responsible for the suppression of those transposable elements. Possible roles of this epigenetic regulation by mir820 and OsDRM2 as well as their agricultural impacts on drought stress resistance will be discussed. *Corresponding Author: Tel , dhjeon73@hallym.ac.kr 243

300 PF-46 김년희, 김송림, 최인찬, 지현소, 이홍석, 이은경, 백정호, 양종목, 안은숙, 김경환 * 식물표현체연구는영상정보를이용하여다양한작물표현형을비파괴적으로분석하는기술로단자엽모델식물인벼를이용하여농업형질특성조사에활용하고자하였다. 벼의영상데이터를획득하고분석알고리즘을통해관심영역 (region of interest, ROI) 의잎면적 (leaf area, LA), 잎폭 (leaf width, LW), 잎색 (leaf color, LC), 영사초장 (projected plant height, PPH), 볼록선체 (convex hull, CVH), 무게중심 (center of mass Y, COMY), 밀집도 (compactness, COMP), 이심률 (eccentricity, ECC) 의파라미터를추출하여형질분석에활용하였다. 실제로, 밀양 23 호와기호벼의재조합유전자식세대 (recombinant inbred line, RIL) 를이용벼초기생장률양적형질분석에사용하었다. 그결과, 초기생장률의경우는 2 주와 4 주의유묘기에서잎면적 (LA) 과영사초장 (PPH) 이염색체 1 번의반왜성단간유전자 (semi-dwarf) 인 sd-1 과염색체 12 번의유전자좌가주요양적형질로조사되었다. 반면에, 영양생장시기인 6 주와 8 주된식물체들은영상추출파라미터들의분석을통해 1 번과 12 번염색체외에도, 2, 3, 7, 9, 11 번염색체에서생장관련양적형질이탐색되었다. 아울러 Phytochrome B T-DNA 삽입돌연변이체 (osphyb) 를이용하여건조저항성형질분석결과, 건조후회복시 osphyb 가대조구보다잎면적, 잎폭이크고식물의수분함량이높아서개체간유의성있는차이가있어건조저항성형질분석시활용가능할것으로판단되었다. 표현체기술은다양한품종및형질특성분석을통해유전자기능대량분석, 육종활용, 자원의선발등에폭넓게적용될것으로기대된다. * 교신저자 : Tel , biopiakim@korea.kr PF-47 Minjin Kim *, Byeongrim Go 국가 R&D 성과의활용을위한기술수요조사및연구개발비투입비율이대학 / 연구소에편중되어있고, R&D 사업화를위한산업체의참여는저조한실정으로 R&D 결과물의사업화는다양한형태의직 간접적상호작용을통해비유형화된형태의성과로창출되고있으나이에관련한지식확산활동노력은여전히부족한실태이다. 또한기술이전사업화과정및성과에중요한영향을미치는요인들을사업화환경으로범주화할수있는기술사업화관련정책및제도, 기술이전사업화를위한인프라 ( 기술평가, 기술금융, 기술거래중개등 ) 로구분되며, 연구개발사업단의경우, 성과확산을위한인프라의구축이필수적인상황이다. 이에본과제에서는농림식품산업의기여및민간생명공학작물개발기업의육성이라는농림식품 R&D 분야의정책적목표달성조기화를위해생명공학작물 R&D 분야에기술전사업화역량을집중하는방안을마련하고자하였다. 본과제추진내용으로농업생명공학연구단 1~2 단계기수행과제및 3 단계과제진단및분석을진행하였으며, 3 단계과제를대상으로사업화전략수립을진행하고있다. 수요기업발굴, 기술자료집제작, 기술설명회개최등으로기술마케팅을지원하고있으며, 수행과제기반 (Tech-Push) 네트워크구축및타겟기업기반 (Market Pull) 네트워크를구축하여, 통합교류회운영및전문가진단으로워킹그룹을운영하여사업화전략수립및공유를지원하고있다. 또한연구단성과홍보및우수사례확산을위한기술가치평가, 맞춤형비즈니스보델개발, 성과및기업통합 DB 구축에대한연구확산프로세스를개발하고자한다. 본과제를통해연구기관 - 기술사업화전문회사간의기술사업화관점에서의기술활용극대화를통한성공모델확보및기술 Value-chain 형성하였으며, 이전대상기술의기술성, 대상기업의재무건정성등경영안정성, 사업추진에따른비즈니스모델의사업성등을종합적으로고려하여성공적인 Business Model 확립하고자한다. 또한기술실시기업대상상용화현황및애로사항을파악하고, 갭중개연구등의지원을통해연구성과의성공적인사업화에견인역할을하고자한다. * 교신저자 : Tel , mjkim1@wips.co.kr 244

301 PF-48 Ye Rim Lee 1, Ji Yun Ko 1, So Young Park 3, Kwon Kyoo Kang 1,2, Yu Jin Jung 1,2* Brazzein is the smallest sweet protein and was isolated from the fruit pulp of Pentadiplandra brazzeana Baillon, native to tropical Africa. From ancient times, the indigenous people used this fruit in their diet to add sweetness to their daily food. Brazzein is 500 to 2000 times sweeter than sucrose on a weight basis and 9500 times sweeter on a molar basis. This unique property has led to increasing interest in this protein. However, it is expensive and difficult to produce brazzein other than in its native growing conditions which limits its availability for use as a food additive. In this study, we have studied high production yields of brazzein protein in transgenic rice cell lines. Brazzein gene was constucted by SWPA2 promoter which specific overexpressed in somatic cell of sweetpotato(kim et al. 2003), and transformed via Agrobacterium methods. Total 160 cell lines introduced by Ti-plasmid vector were selected with growth speed. These cell lines were confirmed by PCR analysis and checked gene expression by RT-PCR and Western blot analysis. These results demonstrate that recombinant brazzein was efficiently expressed in rice cell lines, and that we will be development of pilot-scale system to product natural sweetener. *Corresponding Author: Tel , yuyu1216@hknu.ac.kr PF-49 Sung Un Huh * Autophagy is involved in maintaining cellular homeostasis and regulates biotic/abiotic stresses in eukaryotes. Furthermore, autophagy contributes with both pro-death and pro-survival functions to specific pathogen infections. Several pathogens have evolved strategies to manipulate host autophagy pathways to suppress plant immunity. In this study, the plant autophagy-related gene 8 (Atg8) is coiped and acetylated by Ralstonia solanacedarum effector PopP2 which has an acetyltransferase activity. We further identify a putative Atg8 acetylation amino acid residue via a protein sequence analysis. PopP2 acetylates clade-specific Atg8 proteins but still is able to interact with other clade Atg8 proteins. Together, we demonstrate that autophagy Atg8 might be targeted by bacterial pathogen effector to manipulate host autophagy pathway. Regulation of Atg8 acetylation will provide new approaches in crop engineering. *Corresponding Author: Tel , sungun@kunsan.ac.kr 245

302 PF-50 김민식 1, 윤철원 2, 노현수 1* 1 2 식용버섯은다양한생리활성작용과독특한풍미로인하여, 소비가증가하고있는건강식품이다. 특히, 양송이버섯은세계적으로가장많이생산되고소비되는대표적인식용버섯으로안전성이입증된유용한생물자원이다. 본연구에서는양송이버섯에철분의흡수와항생효과, 항암효과를가진시데로포아를합성하는유전자를도입한형질전환체를제작하고, 이를이용하여시데로포아를대량생산하는물질생산공장으로개발하고자하였다. 이를위하여양송이버섯에서시데로포아생합성유전자의발현을 RT-PCR 뷴석을통하여확인하였다. 그결과철분의존재에의하여발현이조절되는 HapX 와 SidA 의존재를확인하였고, 이들의대량발현을위하여 Agrobacterium tumefaciens mediated transformation (ATMT) 를이용한형질전환체를제작하였다. 형질전환용벡터 DNA 에상기한유전자를삽입하고, 상시발현 promoter 인 GPD promoter 에의하여발현되게하였다. pbgghg-hapx 또는 pbgghg-sida 를양송이버섯과새송이버섯 gill 에각 800 개의형질전환시도를통하여 10 개내외의형질전환체를확보하였다. 확보된형질전환체의유전자서열과발현분석을통하여 HapX, SidA 유전자의발현을분석중이며, 이를통하여시데로포아생산성을검증할계획이다. * 교신저자 : Tel , rohyeon@gnu.ac.kr PF-51 Hwang-weon Jeong *, Jihee Park, Sang Ryeol Park, Inchan Choi, JeongHo BAEK, Young-Joo Seol Massive genomic data of tomato has been generating after tomato genome sequence in 2012 and improvement of NGS technology. But most of phenotype data is still collected by using naked eye. Therefore, production speed of phenotype data is limited and it results in the lack of omics data for new cultivar development in agricultural industry of Korea. In order to improve such circumstance, there are needs for gathering phenotype data using image measurement which is possible to improve production speed. Thus development image-based traits for breeding are important for image measurement. In this study, we will develope image-based traits for image measurement through measuring whole growth period of tomato. Model tomato for the research was selected Micro-Tome which is under 90d for growth period and lower then 20cm height, suitable for image measurement. Development of traits process is as follows: 1) For candidate phenotype traits, gathering phenotype traits are guided based on International Union for the Protection of New Varieties of Plants(UPOV), interest of breeders, plant biology, plant anatomy, plant development and growth. 2) For categorizing candidate traits, indexing specific phenotype trait and it's related. 3) Getting morphological traits using image like plant height, leaf area, fruit size, density of shoots and setting condition for suitable image measurement for plant. 4) Comparing candidate traits and morphological traits, then find useful phenotype traits for breeding and image analysis. Through the development of image-based traits for model tomato, it will be used for phenotype data collection based on image measurement and numeric. In addition to the study, we expanded research area to normal tomato from model tomato for developing "Support system establishment on imaging modality for phenotype and selection of breeding tomatoes". Acknowledgement: This work was supported by a grant from the Next-Generation BioGreen 21 Program (PJ ), RDA, Republic of Korea. *Corresponding Author: Tel , yjseol@korea.kr 246

303 PF-52 Sang-Gu Lee 1*, Seon-Woo Oh 1, Soo-Yun Park 1, So-Young Lee 1, Hwi-Young Park 1, Seong-Kon Lee 1, Young-Soo Chung 2, Hyun-Suk Cho This study was conducted to evaluate the natural variability of total phenolics, phytic acid, and trypsin inhibitors in soybean varieties and to estimate whether the contents and distribution of these compounds are various among soybeans. 14 samples of commercials were cultivated in the three locations which were Daegu, Suwon, and Jeonju of south Korea. Total phenolics were estimated by using gas chromatography-time of flight mass spectrometry (GC/TOF MS) including tert-butyldimethylsilyl (TBDMS) derivatization. High linearity values of r2>0.99 were obtained in the standard calibration curve. Total soluble phenolic acids (free and esterified forms) were characterized to determine the diversity among the phenolic acids. The profiles of 6 phenolic compounds were subjected to statistical analysis, including principal component analysis (PCA). 6 phenolic compounds from the commercial soybeans which were p-hydroxybenzoate, vanillate, syringate, coumarate, ferulate, and sinapate. These phenolic compounds were determined based on the retention time of the standards. PCA allowed for the visualisation of complex data and reveal that the varieties separated from the other PC 1 and PC 2. From this results, it could be concluded that the combined chemometric tools and statistical methods could be useful to the safety assessment for the new biotechnology organisms. *Corresponding Author: Tel , sangulee@korea.kr PF-53 Jung Yeon Han 1, Do Won Yun 2, So Hyeon Back 3, Yong Eui Choi 1* Dammarenediol-II is biologically active tetracyclic triterpenoid, which is basic triterpene skeleton of ginsenoside saponin in Panax species. Here, we constructed the transgenic rice overexpressing Panax ginseng dammarenediol-ii synthase gene (PgDDS) driven by rice endosperm-specific alpha-globulin promoter and confirmed the production of dammarenediol-ii (DD) in rice grain by LC-MS analysis. Interestingly transgenic rice produced not only dammarenediol-ii but also protopanaxadiol (ginseng sapogenin). In Panax giseng, PPD is synthesized via the hydroxylation of DD by CYP716A47 enzyme. It has been known that PPD has a wide range of pharmacological activities. Production of DD and PPD was confirmed by comparison of retention times of total ion chromatogram and mass spectra of their peaks. Non-transgenic rice showed any signals for DD and PPD in rice grains. It is known that rice does not have CYP716 family genes which are lost by evolutionary event. It is interesting that transgenic rice can hydroxylate the DD into PPD, which might be resulted from the hydroxylation activity of unknown intrinsic CYP enzyme in rice. We isolated the putative CYP716B subfamily genes from rice and tested their possible role in hydroxylation activity of DD for the conversion to PPD. *Corresponding Author: Yong Eui Choi, Tel , yechoi@kangwon.ac.kr 247

304 PF-54 선현진 1, 정옥철 1, 강홍규 1, 진일두 1, 이동희 2, 정혜린 3, 이효연 1,3, 서석철 1,3* 한국잔디의일종인들잔디 (Zoysia japonica Steud.) 는우리나라를포함한동아시아지역과대부분의온대지역에분포하고있는난지형잔디중의하나이다. 잔디는경관보호, 토양보호, 신변보호등의목적으로이용되고있으며, 도로, 주택, 공원, 스포츠경기장등으로그활용범위가점차확대되고있다. 이와더불어잔디관련산업규모가확대되고있으며, 병해충저항성, 제초제저항성, 환경스트레스내성, 왜성, 녹기연장등다양한특성을가지는신품종잔디의개발이요구되고있다. 잔디의신품종개발에는주로전통육종방법이이용되어왔으나, 전통육종을통한품종개량은오랜시간이걸릴뿐만아니라개량가능한형질에도한계가있으므로, 최근에는생명공학기술을이용한신품종개발연구가증가하고있다. 본연구팀은선행연구를통하여제초제저항성들잔디를개발하였고, 최근에는이제초제저항성들잔디를부본으로야생형금잔디와교배하여제초제저항성과왜성형질을가지는신품종잔디를선발하여육성하였다. 신품종잔디는들잔디에비해초장및엽장이짧은왜성형질을나타내었고, 엽폭이좁은세엽의특성을가지고있었으며부본의특성인제초제저항성도가지고있음을확인하였다. 이와같이육성된신품종잔디는외래유전자가도입된 GM 작물이므로상용화를위해서는각종환경위해성평가를수행하여야한다. 본연구에서는 GM 작물의환경위해성평가항목중 GM 잔디의주변생태계에미치는영향에대한평가를수행하고자하였다. 현재까지신품종잔디의집수정내의수서미생물로의수평적인유전자전달여부를분석하였으며, 그결과, 도입유전자의수평적유전자전달은일어나지않은것으로확인되었다. 이와더불어신품종잔디추출물을사용하여제브라피쉬의생육에미치는영향을평가하고있으며, 향후이벤트잔디의곤충상에미치는영향, 토양세균의다양성에미치는영향등다양한평가를수행하여신품종잔디가주변생태계에미치는영향을종합적으로분석할계획이다. * 교신저자 : Tel , scsuh@jejunu.ac.kr PF-55 선현진 1, 정옥철 1, 강홍규 1, 진일두 1, 이동희 2, 서석철 1,3, 이효연 1,3* 들잔디 (Zoysia japonica Steud.) 는난지형잔디의일종으로우리나라를포함한동아시아지역에자생하고있으며, 공원, 묘지, 경사면녹화등에이용되어왔다. 최근에는잔디의이용범위가스포츠경기장, 골프장, 개인주거지등으로점차확대되고있으며, 이에따라다양한특성을가지는신품종잔디의개발이요구되고있다. 신품종잔디에요구되는주요형질로는제초제저항성, 환경스트레스내성, 병해충저항성, 왜성, 녹기연장, 시각적품질개선등이있으며, 이들형질의대부분은전통적육종방법으로개발하기에는한계가있다. 본연구에서는제초제저항성을가지면서초형, 질감, 밀도등시각적품질이개선된신품종잔디를개발하기위하여선행연구에서개발된제초제저항성들잔디와야생형금잔디를교배하여신품종잔디를선발 육성하였다. 선발된교배종잔디는제초제저항성을가지고있으므로잡초관리가용이하고, 시각적품질이우수하기때문에경제성도높을것으로기대되고있다. 그러나개발된잔디는생명공학기술이활용된 GM 작물이므로상용화를위해서는안전성심사에서요구되는각종환경위해성평가가반드시수행되어야한다. 따라서본연구에서는국내의 GM 작물개발자들이활용할수있는환경위해성평가모델을개발함과동시에신품종잔디의환경위해성평가연구를수행하고자하였다. 신품종잔디를증식하여농업적특성평가구역, 유전자이동성평가구역, 잡초화가능성평가구역을각각조성하였고, 연구계획에따라순차적으로환경위해성평가를수행할계획이다. 현재까지신품종잔디의초장, 엽장, 엽폭, 엽각도등의농업형질을조사하였으며, 그결과, 신품종잔디는초장, 엽장, 엽폭등이들잔디에비해작은왜성및세엽형질을가지고있었으며, 잔디의질감도들잔디에비해개선된특성을나타내었다. 본연구를통해육성된교배종잔디는잔디관리에있어서매우중요한요소들인예초및잡초제거에소요되는비용및노동력의절감에기여할수있을것이며, 우수한시각적품질은소비자들이선호하는형질이므로경제적가치도매우높을것으로기대된다. * 교신저자 : Tel , hyoyeon@jejunu.ac.kr 248

305 PF-56 박수윤 1*, 이경민 1, 오성덕 1, 서상재 2, 오선우 1, 이상구 1, 이성곤 1, 조현석 유전자변형 (Genetically Modified, GM) 콩의재배는꾸준히증가하여 2016 년에전세계에서재배되는콩중 78% 가 GM 콩인것으로조사되었다. 이는전체재배된 GM 작물중 50% 에달하는규모로, GM 콩의경제적 상업적가치가높음을나타낸다. GM 콩이국내에식품및농업용으로수입승인되고있어, 이의비의도적환경방출에대한조사및연구의중요성이커지고있다. 때문에본연구에서는 GM 콩과야생콩과의교잡에의해발생된교잡콩의대사체분석을통해교잡종을판별할수있는검출법개발을목표로하였다. 이를위해, 기능성물질생성증가를목표로개발된비타민 A 강화 GM 콩과야생콩을대상으로대사체변화를확인할수있는분석체계를먼저구축하고, 이후얻게될교잡종에이를적용할계획이다. GM 콩의목적성분인비타민 A 의전구체인카로티노이드를분석하기위해 HPLC 를이용하였다. 카로티노이드 (carotenoids) 는자연계에약 500 여종이존재하며이들중약 10% 만이레티놀 (retinol) 로전환될수있는것으로알려져있다. 분석결과, 비타민 A 강화콩의목적성분인베타 - 카로틴 (beta-carotene) 은야생콩에서상대적으로미량존재하였고, 관련된주요대사물질인루테인 (lutein) 은모품종 ( 광안콩 )>GM 콩 > 야생콩순서로함량차이가있었다. 이러한기능성 2 차대사물질의생합성경로를추적하기위해서는 1 차대사물질프로파일링연구가필수적이다. 현재, GM 작물의목적성분과관련된더많은주요대사물질을분석할수있는체계를확대구축하고있으며, 더불어 GC-TOFMS 를이용하여친수성 1 차대사물질을분석할수있는체계를콩에맞춰구축해나가고있다. 대사체학을이용한포괄적인대사물질프로파일데이터확보는기능성강화 GM 작물의비의도적환경방출로인해발생가능한교잡종의대사변화를연구하는데중요한역할을할수있을것이다. * 교신저자 : Tel , psy22@korea.kr PF-57 Hwi-Young Park, Sang-Gu Lee *, Seon-Woo Oh, Soo-Yun Park, Seong-Kon Lee, Hyun-Suk Cho 생명공학기술의급속한발전에따라새로운생명공학작물이지속적으로개발되고있다. 이와함께생명공학작물의비의도적이며예기치못한영향평가를위해서실질적동등성개념을기본으로한안전성평가가이루어지고있다. GM 작물을식품으로이용할경우코덱스국제식품규격위원회 (Codex Alimentarius Commission) 의규정및가이드라인에따라인체건강위해에대한안전성을검증받도록하고있다. 특히알레르기유발성은코덱스의평가방법에서도별도의항목을둘정도로중요하게여기고있다. 알레르기중식품알레르기는식품이나식품첨가물을섭취한후발생되는이상반응중면역기전에의해발생되는경우를지칭하며, 대부분의사람에게는섭취하여도문제가되지않는성분이일부민감한사람에게는섭취시비정상적인면역반응을일으켜큰문제가될수도있기에, GM 작물에도입된새로운단백질의알레르기유발성평가는필수적이다. 따라서본연구는오메가 -3 함량을증대시킨기능성콩형질전환체의작물학적가치평가를위해, 기존에재배되고있는작물과의영양성분및항영양소비교분석뿐만아니라알레르기유발가능성평가를실시하고자한다. 이를위해서이미알려져있는알레르겐과의아미노산서열상동성을확인하고인공소화액, 열안정성검정등물리화학적특성시험법을개발하여 GM 콩품종뿐만아니라새로운생명공학작물의알레르기유발가능성을확인하고나아가 GM 작물안전성평가에대한자료를제공할수있을것으로기대된다. *Corresponding Author: Tel , sangulee@korea.kr 249

306 PF-58 So-Young Lee *, Seon-Woo Oh, Hwi-Young Park, Soo-Yun Park, Sang-Gu Lee, Seong-Kon Lee, Hyun-Suk Cho This study was to evaluate the nutritional profiles of 12 kinds of hot pepper fruits cultivated in the two locations which were Youngyang of Kyeongbuk and Imsil of Jeonbuk for two years. All of these peppers were cultivated by standard and conventional agricultural practice. Commercial varieties of hot peppers were analyzed to compare the nutritional profiles according to the different environmental conditions. The ph of soil, annual rainfall, and weather temperature were investigated for the environment differences. The micronutrients of vitamine, minerals and fatty acids were investigated for the environmental effects. The contents of minerals were not different between two locations but the amino acid contents were different significantly (p<0.05) between two locations. Micronutrients were affected more by the environment conditions such as cultivated years and locations than kinds of varieties. The fatty acid profile showed that linoleic acid, palmitic acid, oleic acid, stearic acid and linolenic acid (0.2~0.5% of total) as the most abundant fatty acids followed lauric acid, arachidic acid, and behenic acid ( % of total). Analyses of mineral content indicated that the most abundant mineral was potassium, followed by magnesium, calcium, iron, zinc, sodium and manganese. These data could be used in the safety assessment of new plants developed by the biotechnology by comparing the substantial equivalence. *Corresponding Author: Tel , lsy0321@korea.kr PF-59 정하나 2, 좌지방 2, 손지희 2, 선현진 1, 양대화 1, 강홍규 1*, 이효연 1,2 1 2 들잔디 (Zoysia japonica) 를포함한난지형조이시아 (zoysiagrass) 잔디는우리나라, 중국, 일본, 동남아시아, 호주등지에자생하고있다. 현재조이시아잔디는아시아뿐만아니라미국, 유럽까지전파되어전세계적으로정원, 공원, 골프장, 야외스포츠공간등에서널리이용되고있으며경제적가치가높은산업성농작물로분류되고있다. 잔디는재배혹은잔디밭조성시에잡초관리비용이매우높으므로제초제내성은경제적가치가매우높은형질이다. 본연구는글루포시네이트저항성유전자변형들잔디 JG21(Z. japonica) 와비변형금잔디 (Z. matrella) 의종간교잡을통해육성된고품질제초제내성교배종잔디계통 (JG21-MJ) 의 LMO 안전성을평가하기위한시험연구이다. 당해연도 (2018 년 ) 에는도입유전자주변염기서열의안정성검정, T-DNA 의복제수및도입특성검정, T-DNA 의 backnone DNA 의비의도적 DNA 도입검정, 교배종잔디 (1 세대 ) 의수분및종자수확을포함한다. 현재연구를위한식물재료인 JG21 의모본들잔디, JG21, 교배친금잔디, 교배종신품종잔디계통등을확인하고관리하는기본적인일들을수행하였다. 또한차후고품질호모계통잔디의선발, 복수세대에서의도입유전자안정성평가등을위해교잡 1 세대식물의자가수분종자를채종하였다. 교배종잔디내도입 DNA 가삽입된게놈상의위치를평가하기위해도입 DNA 의양쪽주변염기서열의특성을분석하였다. * 교신저자 : Tel , honggyu@jejunu.ac.kr 250

307 PF-60 김진주, 박성임, 윤호성 * 지구환경변화로인해발생하는다양한환경스트레스는작물의수확량감소에직접적인원인으로작용한다. 본연구에서는환경스트레스에내성이있는기능이알려지지않은벼유래의유전자 (AK102606) 와 1, 2 단계에서검정된배추 (Brsasica rapa sp.) 유래의비타민 C 환원유전자 (BrMDHAR) 의기능검정및생체내유전자의특성을심도있게규명하고글로벌화가능신규유용유전자를개발하고자한다. 1 차년도 (2018 년 ) 개발목표중하나인 AK 와 BrMDHAR 형질전환체의 GMO 포장지에서의재해내성및수량성분석을위해각 개체씩이앙하였다. 또한 GMO 포장지의비점오염원분석및날씨모니터링을위해온도계를설치하여최대 최저기온을측정하고, 관개수의이온함량측정을진행중이다. 추가적인유전자기능검정을위해 AK 와 BrMDHAR 두유전자를콩에도입시키기위해형질전환에이용되는벡터인 pentr-topo vector 로클로닝을완료하였다. 1 차년도남은기간동안 AK 벼형질전환체의전사체기반유전자발현및대사체물질분석을통해환경스트레스내성메커니즘에대한심층적인분석을진행할계획이다. * 교신저자 : Tel , hsy@knu.ac.kr PF-61 Ye Rim Lee 1, In Hye Lee 2, Yeo Jin Jung 1, So Young Park 3, Kwon Kyoo Kang 1, Yu Jin Jung 1* Brazzein is the smallest sweet protein and was isolated from the fruit pulp of Pentadiplandra brazzeana Baillon, native to tropical Africa. From ancient times, the indigenous people used this fruit in their diet to add sweetness to their daily food. Brazzein is 500 to 2000 times sweeter than sucrose on a weight basis and 9500 times sweeter on a molar basis. This unique property has led to increasing interest in this protein. However, it is expensive and difficult to produce brazzein other than in its native growing conditions which limits its availability for use as a food additive. In this study, we report high production yields of, brazzein protein in transgenic rice plants. An ORF region encoding brazzein and driven by the 2 x CaMV 35S promoter was introduced into rice genome (Oryza sativa Japonica) via Agrobacterium-mediated transformation. After transformation, 17 regenerated plant lines were obtained and these transgene-containing plants were confirmed by PCR analysis. In addition, the selected plant lines were analyzed by Taqman PCR and results showed that 9 T0 lines were found to have a single copy out of 17 transgenic plants. Moreover, high and genetically stable expression of brazzein was confirmed by Western blot analysis. These results demonstrate that recombinant brazzein was efficiently expressed in transgenic rice plants, and that we have developed a new rice variety with a natural sweetener. *Corresponding Author: Tel , yuyu1216@hknu.ac.kr 251

308 PF-62 김도훈 1*, 김종민 거미피브로인단백질을발현하는당대사개선용형질전환벼의이벤트육성과제 2 형당뇨치료효과의검증자료확보및제시하고자본연구를수행하였다. 연구결과를요약하면거미피브로인단백질은항산화작용, 간기능개선작용, 인슐린분비촉진작용, 혈중콜레스테롤상승억제작용, 신경계질환예방및개선작용등의임상적효과가입증되어있다. 당대사개선용형질전환벼를대상으로 genotyping 을이용하여유전자가안정적으로도입된호모라인을선발하였고, southern blot 분석을실시하여형질전환벼에도입된유전자가 single copy 로존재하고있음을확인하였다. 또한, northern 및 western blot 분석을실시하여형질전환벼에도입된유전자가안정적으로강하게발현되는것을확인하였고, 목적단백질인피브로인단백질이안정적으로합성되는것을확인하였다. 선발된형질전환벼를경북대학교 GM 포장에이앙하여바스타처리를통한고정계통선발, 농업형질안정계통및우수계통선발및검정교배에의한고정계통제작을통한유전분석을실시중에있다. Murine fibroblast cell line, NIH 3T3 cell 을대상으로 cytotoxicity, MTT assay 및 caspase-3 activity 분석하여거미실크피브로인단백질이세포에대한독성이없으며및세포사를유도하지않는것으로확인되었고, Murine macrophage cell line J774 를이용하여 mcrophage stimulation 실험을통해거미실크피브로인단백질이 proinflammatory mediator 및 cytokines 의생성을유도하지않는것으로확인되었다. 자연발생제 2 형당뇨병모델쥐 (BKS.Cg-m + /+ db mice) 에형질전환벼를급이하여당뇨관련에너지대사과정조사, 인슐린신호전달과정조사및당뇨관련각조직에서병태생리변화조사실험을진행중에있다. 이런결과들로볼때형질전환벼를의약소재로활용이가능할것으로예상되어진다. * 교신저자 : Tel , dhkim@dau.ac.kr PF-63 조윤성 *, 임명호, 신공식 1994 년과육연화지연특성을주입한토마토, FLAVR SAVR TM 가상업용으로최초승인된이래현재까지 11 개의유전자변형 (GM) 토마토가상업용으로승인을받았다 (2018 년 6 월현재, ISAAA). 최근외국에서수입된유채, 면화종자에 GMO 가혼입되어문제가되고있는상황에서 GM 토마토의유입에대비하기위해 GM 토마토를검출하기위한시스템을개발하고자하였다. 1 차적으로토마토의유전자변형여부를검정하기위한 universal marker 개발에는 cauliflower mosaic virus 35s (CaMV 35s) promoter, Agrobacterium tumefaciens 의 nopaline synthase (nos) terminator, neomycinphosphotransferase (nptii) 를이용한다중 pcr 을개발하였다. 또한 GMO 로판별된토마토를대상으로이벤트를판별하는방법을개발하였다. 여기에는각이벤트의고유한유전자인 1-aminocyclopropane-1-carboxylic acid (ACC) synthase, 1-aminocylopropane-1-carboxylic acid deaminase (accd), S-adenosylmethionine hydrolase (SAM) gene, anti-sense ethylene-forming enzyme (anti-efe), polygalacturonase (pg) gene, Cry1Ac, cucumber mosaic virus coat protein (CMVcp) 를목표유전자로이용하였다. 이벤트선별용검정에는 universal marker 를이용한다중 pcr 의결과에따라사용되는목표유전자의조합을달리하였다. * 교신저자 : Tel , younsung@korea.kr 252

309 PF-64 Hyun Suk Cho 1, Hye Jeong Kim 1, Wan Woo Yeam 1, Jin Sol Park 1, Jae Yong Han 1, Hyun Uk Kim 2, Young-Soo Chung 1* 1 2 α -linolenic acid, an omega-3 fatty acid, is taken as health supplement. For economic reasons, α -linolenic acid is mainly extracted from fish. We have tried to transform soybean cultivated globally to produce competitive crops. PfFAD3-1 gene, which is derived from Physaria fendleri called Lesquerella plant, strongly produces α -linolenic acid. PfFAD3-1 gene was introduced into soybean by Agrobacterium-mediated transformation method. In transgenic plants (T 0 ), PfFAD3-1 gene was identified by using PCR and Southern blot analysis of T-DNA. In addition, content of α -linolenic acid in the transformed seeds (T 1) was confirmed by gas-chromatography analysis, and α -linolenic acid content was measured 6-times higher than wild type soybean seeds. Agronomic characters including plant height, the number of nods per plant, branches per plant, pods per plant and total seed weight of 12 transgenic lines (T 1 ) with high α -linolenic acid content were investigated in the GMO field. As a result, the yield was increased in harvested T 2 seeds. *Corresponding Author: Tel , chungys@dau.ac.kr PF-65 Minhee Kang 1, Eunhye Goo 1, Jae Yun Lim 1, Jinwoo Kim 2, and Ingyu Hwang 1* 1 2 Burkholderia glumae is the causal agent of bacterial panicle blight and has a single LuxI-R type quorum sensing (QS) system. N-octanoyl homoserine lactone (C8-HSL) is biosynthesized by signal synthase, TofI, and is recognized by its cognate receptor, TofR. TofR complexes with C8-HSL to regulate production of toxoflavin, one of the major virulence factors of B. glumae. We collected 58 B. glumae isolates from diseased rice panicles, broken rice, and solanaceae crops. The differences in virulence of the isolates were determined, and its whole genome sequences were analyzed. Most isolates have similar genome structures of B. glumae BGR1 possessing two chromosomes and four plasmids. However, five of the isolates were significantly different from BGR1; two isolates had one big merged chromosome, and one isolate had significant gene rearrangement between two chromosomes. In the other two isolates, the genome structure of chromosome 1 was closely related to that of other Burkholderia species. Most of the strains isolated from broken rice did not produce toxoflavin. These toxoflavin-negative strains contained an IS element insertion in the promoter region of toxoflavin biosynthetic gene cluster. These results suggested that the Burkholderia species isolated from various inoculum have genome plasticity and it affects bacterial virulence. *Corresponding Author: Tel , ingyu@snu.ac.kr 253

310 PF-66 Wen-Xing Hu 1, Jung-Kyu Kim 1, Hye-Kyoung Ju 1, Hanhong Bae 2, Leslie L. Domier 3, Hyoun-Sub Lim 1* Several CRISPR-Cas9 orthologues have been used for genome editing in plant or animal systems, etc. Recently, a small Cas9 orthologue derived from Campylobacter jejuni (CjCas9) has been shown to be functional for efficient genome editing in vivo in animals. Here we used a potexvirus viral vector to efficiently express CjCas9 proteins systemically in Nicotiana benthamiana, with confirmation of expression in the newly-formed upper leaves demonstrated by RT-PCR. The single-guide RNA (sgrna) required for activity is packaged in the viral vector multiple cloning site, flanked by hammerhead ribozyme sequences at both sides, such that the sgrna is able to precise self-cleavage at these specific site to release the mature sgrna sequence. Furthermore, a Theophylline-dependent hammerhead ribozyme switch is also used to control the release of the sgrna. Our work is targeted to production of genome edited seed from the plant viral-cas9 infected crop, which is expected to be a more convenient, cost-effective, and high-efficiency genome editing method. *Corresponding Author: Tel , hyounlim@cnu.ac.kr PF-67 이향기 *, 송민경 일반적으로소비자들은식품안전성문제에있어매우민감하게반응하고충분한정보를제공받지못하거나불확실한정보를제공받는경우소비자들의식품안전에대한불안감은커진다. 소비자들의우려를불식시키거나위험인지수준을낮추기위해서는정확한정보가중요하다. 국내에서의유전자변형식품에대한사회적인식은부정적인경향이높다. 따라서오피니언리더그룹의정보전달방법과전달할내용을맞춤형으로정보제공을통해오피니언리더그룹에의한 GMO 인식이왜곡되지않도록하고올바른 GMO 인식을확산시킬필요가있다. 본조사에서는 19 대국회의원 (2015 년 )~20 대국회의원 (2017 년 ) 에걸쳐 GMO 에대한국회의원인식도조사를실시했다. 주요정책을수립하는국회의원은오피니언리더로서국민여론형성에매우큰영향을미치고있어국회의원의 GMO 인식도파악을통해정확한정보제공을하고올바른 GMO 정책수립을위한기초자료로제공하고자한다. 국회의원의 GMO 에대한인식도는 2015 년 42.4%, 2016 년 37.9%, 2017 년 46.9% 로 50% 에미치지못하고있고 GMO 에대해관심을갖는이유는 GMO 의안전성여부 (36.1%) 와국민의불안감 (25.3) 이높게나타났다. GMO 에대한지식정도는평균정답률이 2015 년 54.5%, 2016 년 52.5%, 2017 년 53.5% 로매년비슷한응답을보여 GMO 에대한지속적인정보전달이필요한것으로나타났다. 국내유전자변형기술에대한국회의원의인식은 식량위기를극복할수있는대안이될수있다 는응답이 2015 년 23.6%, 2016 년 21.6%, 2017 년 28.5% 로식량확보대안의응답이 10 명중 3 명이다. 환경파괴, 인체위해등이우려된다 는응답은 2015 년 32.5%, 2016 년 40.5%, 2017 년 29.8% 로 2017 년도에는다소감소한경향을보였다. GMO 표시제확대법안에대해찬성하는국회의원의의견은 2015 년 93.1% 로높았으나 2016 년 79.7%, 2017 년 74.1% 로다소감소하는경향을보였다. GMO 표시제확대법안에반대하는이유는 표시로인해소비자의불안을야기할수있다 와 GMO 단백질이남아있지않는경우는검출이불가능하다 였다. GMO 확대법안에찬성하는이유는 소비자알권리보장하기위해서 (82.7%) 이높은응답을보였다. 우리나라의 GM 작물개발에대해서가장많은응답은 GM 작물중식용이아닌사료나화훼, 의료용등의 GM 작물로만국한해서개발해야한다 로 2015 년 38.4%, 2016 년 41.9%, 2017 년 34.6% 이고, 그다음 GM 작물개발은자국의농업환경을바꿀수있고식량을안정적으로확보할수있으므로우리나라도 GM 작물을상업화시켜야한다 로응답율이 2015 년 15.8%, 2016 년 14.9%, 2017 년 25.9% 로 2017 년이 2016 년보다 11% 증가했다. GMO 에대한의사소통시우선대상으로는대국민정보제공의응답율이가장높아 2015 년 58.3%, 2016 년 62.5%, 2017 년 39.0% 이고, 국회의원을포함한오피니언리더는 2015 년 9.6%, 2016 년 13.8%, 2017 년 33.8% 로오피니언리더를대상으로한정보제공의필요성이점점증가하는것으로나타났다. 필요정보로는 식품으로서의안전성에대한정보 (61.3%) 와 GM 기술의기대와우려에대한정보 (15.1%) 로나타났다. 국회의원의 GMO 인식도는 50% 이하로여전히낮은것으로나타나지속적인정보제공이필요한것으로사료된다. * 교신저자 : Tel , lhk1234@hanmail.net 254

311 PF-68 이범규 * 이용자중심의농업생명공학정보제공효율화방안도출과생명공학관련오프라인정보제공분석을통해농업생명공학에대한효율적정보제공프로그램을개발하고자연구를수행되었다. 온라인농업생명공학정보제공효율화방안도출을위한이용자의니즈 ( 필요정보 ) 를분석하기위해설문조사를수행하였다. 농업생명공학정보제공실태에대한설문조사는농업생명공학연구자와일반인 ( 성인, 학생, 농업인 ) 으로대상을구분하여진행하였으며, 농업생명공관련연구자 450 명및중고등학생 400 명을대상으로수행되었다. 현재설문조사결과를분석중에있으며, 향후연구자 200 명및학생 400 명에대한설문조사를추가로진행할예정에있다. 생명공학관련오프라인정보제공분석을통한효율적정보제공프로그램개발을위해청소년직업박람회학생참석자 100 명을대상으로 DNA 분리체험및농업생명공학소개를수행하였다. 향후중 고생및대학생을대상으로한농업생명공학오프라인교육을추가적으로 4 회이상수행할계획이다. 또한일반성인소비자를대상으로농업생명공학교육을실시하고피드백을통한정보제공프로그램효율화방안도출도수행할계획이다. * 교신저자 : Tel , leebk@jj.ac.kr PF-69 Junho Lee 1*, Md Reyazul Islam 1 and Inhwan Hwang 1,2 1 2 Various approaches have been attempted to achieve high production of recombinant proteins in plant tissues, such as strong promoter design, specific 5 untranslated region (UTR) insertion, utilization of virus RNA replication system and so on. In this study, we investigated Gal4/Upstream Activation Sequence (UAS) and post transcriptional gene silencing (PTGS) suppressor to utilize them synergistically for high production of recombinant proteins in plant tissues. Compared with the construct driven by the CaMV 35S promoter that is generally used for protein overexpression in plant tissues, coexpression of the Gal4-VP16 led to more than 2 times higher expression level increase of the target protein regulated by the UAS promoter in Arabidopsis protoplasts. However, Gal4-VP64m induced protein expression with only similar amount of the target protein driven by the 35S promoter, indicating that 4 repeats of VP16 minimal domain are not sufficient to increase protein expression significantly as reported in previous researches. The PTGS suppressor P38 coexpression also enhanced the target protein expression level effectively and combination with Gal4/UAS system accomplished much higher protein production, suggesting that the synergic effect of these independent approaches can be usefully applied for high production of recombinant proteins. Taken together these results, we concluded that each approach using Gal4/UAS system or PTGS suppressor is remarkably effective for high production of recombinant proteins in plant tissues and sophisticated utilization combining them can be the useful strategy to increase recombinant protein expression level even more dramatically. *Corresponding Author: Tel , cowboy98@postech.ac.kr 255

312 PF-70 Jin-Hyun Kim 1, Joo-Seok Park 2, Yoram Choi 2, Min-Gyun Jeong 2 and Hong-Kyu Choi 3* Comparative genome analysis is a powerful approach to look into the genomic organizations among different, but evolutionary related, species, to predict function of certain genes of interest and to interpret evolutionary relationships between compared species. For such reasons, development of efficient and automated bioinformatic visualization tool is essential in this research field. To achieve this goal, we intended to construct an interactive and flexible bioinformatic interface for the comparative analysis focused on legume genomes, named LegCompara. This platform consists mainly of two parts: a web-based user interface and corresponding relational databases. The database harbors a diverse array of genomic information (e.g., functional annotation, ortholog groups) for seven legumes (M. truncatula, G. max P. vulgaris, C. cajan, V. radiata, C. arietinum) and two model plants (A. thaliana, O. sativa). This genome browser, unlike other traditional genome browsers, was designed for researchers to dynamically interact with user interface, so it can navigate multiple chromosomes of different or same species simultaneously, resulting in genome-wide and/or regional comparisons by depicting corresponding syntenies with either blocks or lines between orthologous regions or genes. It is expected that LegCompara may provide researchers and breeders with useful resources for more efficient and user-friendly comparative genome analysis. *Corresponding Author: Tel , hkchoi@dau.ac.kr PF-71 Joo-Seok Park 1, Jin-Hyun Kim 2, Yoram Choi 1, Mingyun Jeong 1, Hong-Kyu Choi 3* Genome-wide transcriptome analysis is one of the most powerful means to gain a broad and deep insight into the molecular mechanisms that underlie dynamic interactions among numerous genes in organisms. Although several bioinformatic platforms for gene expression profiling have been developed for individual species, platform for cross-species transcriptome analysis is not currently available. We employed the technical concept of translational genomics between different species and aimed to build the platform in user-friendly manner. This DB-linked platform, named LegExpress, harbors wide array of transcriptome data for three representative species with relatively the most comprehensive gene expression information, including Glycine max, Medicago truncatula and Arabidopsis thaliana. All these expression data were collected from publically available ArrayExpress( and composed mainly of Affymetrix GenChip data. Raw data were processed to select high-quality transcriptome data and normalized by the RMA standardization method. We developed a program for visualization of the data and organized the user interface according to suitable criteria, such as organs, developmental stages, time courses and different stimuli (e.g., hormones, biotic/abiotic stresses). It is anticipated that LegExpress may play a useful role for breeder/researcher-friendly transcriptome analysis platform and can be applied to design breeding programs through helping breeders discover trait-associated genes. *Corresponding Author: Tel , hkchoi@dau.ac.kr 256

313 PF-72 이운석 1, 김형석 1, 김준식 2* 1 2 작물의 3 차원표현체를자동추출하기위해다중시점영상을활용하는경우, 카메라의자세및초점거리등을정밀하게얻어내는과정이필요하다. 한국과학기술연구원에서개발하고있는다중시점영상취득시스템은정밀하게제어되는회전로봇팔에 RGB 카메라, IR 카메라, 깊이카메라 (depth camera) 등이설치되어있어단시간내에단일작물의다중시점영상을취득할수있는데, 특히 IR 카메라와깊이카메라의경우적외선영상을취득하여활용한다. 본연구에서는 chamber 내에설비된로봇팔과그위에장착된 IR 카메라의위치를정밀하게추정하는방법을개발하였다. 이를위해 Chamber 벽면에전방향으로입사광을반사하는 retro-reflective 재질의 marker 를배치하고그위치를 3 차원레이저로미리정밀하게측정해둔다. IR 카메라의뒷면에배향된조명에의해전반사마커가 IR 영상에취득되게되며, 미리측정해둔 3 차원위치정보와영상내에서검지된마커의위치를이용해서카메라의자세및변수를측정한다. 이과정은제한된화각을가지는카메라의경우정확히자세를추정하지못하는문제가발생하므로, 본연구에서는이에덧붙여영상취득시의로봇팔의자세와팔끝단에고정된카메라의자세변동을동시에추정하여더욱정밀한자세추정을얻을수있다. 이때, 보통 1 축의회전을가하는로봇운동의특성을고려한전방자세최적화를수행하여노이즈에강인하고정확한카메라자세추정이가능하다. 다중깊이영상을단일모델로정합하는실험을통해 IR 카메라의자세추정정밀도는재투영오차를기준으로 1 pixel 이내이며 3 차원에서도기존의방법에비해정밀한위치정합결과를얻어작물의 3 차원모델을빠른시간안에얻을수있음을보였다. * 교신저자 : Tel , junsik.kim@kist.re.kr PF-73 Yong-Sung Park, and Cheol-Won Yun * FgFtr1 and FgFtr2 are putative iron permease and FgFet1 and FgFet2 are putative ferroxidase of Fusarium graminearum. They have high homologies to iron permease, ScFtr1 and ferroxidase, ScFet3 of Saccharomyces cerevisiae respectively at the level of deduced amino acids. Interestingly, the genes encoding iron permease and ferroxidase were localized on the same chromosome in the manner of FgFtr1/FgFet1 and FgFtr2/FgFet2. The GFP-fused versions of FgFtr1 and FgFtr2 showed normal functions when compared with FgFtr1 and FgFtr2 in S. cerevisiae system, and the cellular localizations of FgFtr1 and FgFtr2 in S. cerevisiae depended on the expression of their putative ferroxidase partners, FgFet1 and FgFet2 respectively. Although FgFtr1 was found on the plasma membrane when FgFet1 and FgFtr1 were co-transformed in S. cerevisiae, most of the FgFtr1 was found in the vacuole when FgFet2 was co-expressed. Furthermore, FgFtr2 was found on the vacuolar membrane when FgFet2 was co-expressed and the vacuolar iron contents were increased when FgFtr2 was deleted. From the two-hybrid analysis, it was confirmed that FgFtr1 and FgFet1 interacts physically and same result was found between FgFtr2 and FgFet2. Iron-uptake activity also depended on the existence of the respective partner. Finally, the FgFtr1 and FgFtr2 were found on the plasma membrane and vacuole respectively in the F. graminearum. Taken together, these results strongly suggest that FgFtr1 and FgFtr2 in the F. graminearum encode the iron permeases of the plasma membrane and vacuole membrane, respectively, and require its specific ferroxidases to carry out normal function. Furthermore, this report suggests that the reductive iron uptake system is conserved from yeast to filamentous fungi. *Corresponding Author: Tel , cheolwony@korea.ac.kr 257

314 PF-74 Sung Aeong Oh 1*, Thi Hoai Thuong Nguyen 1, Hyo Jin Park 1, Mingmin Zhao 2, David Twell 2, Sang Ju Lee 3, Jeong Heo Kim 3, and Soon Ki Park Correct development of gametophytes is central for sexual reproduction in flowering plants. The male gametophyte consists of two sperm cells and a vegetative cell which are formed through a meiotic and two mitotic cell divisions under an elaborate genetic program. In order to further understand the genetic regulation underlying the male gametophytic development, we adopted a forward genetics approach and isolated a mutant line, AP28-23, which dehisces a high level of aborted pollen grains. Genetic analysis revealed that the mutant allele is transmitted normally through the female but rarely through the male. Developmental analysis further showed that mutant microspores develop normally to polarized microspore stage but fail to enter pollen mitosis I and gradually degenerate. Map-based cloning and complementation analysis showed that the mutant pollen phenotypes are caused by a 2-amino-acid deletion in the R2R3 domain of a MYB transcription factor family member. RT-PCR and promoter-gus reporter analyses showed that this MYB family member is expressed in a male-specific manner. In addition, the MYB-RFP fusion protein driven by the native promoter is detected specifically in the nuclei at the microspore stages and no longer detected after pollen mitosis I. Taken together, we identified a microspore-specific R2R3 MYB transcription factor essential for microspore division which is a determinative step for the male gamete production in Arabidopsis. *Corresponding Author: Tel psk@knu.ac.kr PF-75 Sung-Aeong Oh 1*, Hyo-Jin Park 1 and Soon-Ki Park 1 1 Flowering plants proliferate through double fertilization mediated by the male and female gametophytes. The male gametophyte consisting of two sperm cells as the male gametes and a vegetative cell to generate the pollen tube is formed through an elaborate genetic control. To expand our understanding on the genetic control during pollen development, we morphologically screened mature pollen grains from a mutagenized pool. As a result, we isolated a mutant line, AP26-09, displaying a range of abnormal pollen phenotypes at maturity, which include pollen grains with the germline cells remained against the pollen wall. Genetic analysis showed that the female transmission of the mutation is normal but the male transmission is highly reduced. Developmental analysis revealed that the callose surrounding the germ cell is abnormally accumulated and the germ cell detachment from the pollen wall is impaired. By a map-based cloning and complementation analysis we show the pollen defects in the AP26-09 mutant line arise from a genetic lesion in a Domain of Unknown Function 707 (DUF707) gene. Publicly available microarray data and our expression analysis show that the DUF707 gene is broadly expressed in both somatic and male gametophytic cells. Importantly, promoter fusion and protein fusion experiments show that the DUF707 is specifically expressed in the germ line cells during pollen development. Our results show that the function of this DUF707 member is required for the germ cell to migrate inward after pollen mitosis I by ensuring correct callose metabolism in the germ cell. *Corresponding Author: Tel psk@knu.ac.kr 258

315 PF-76 Irisappan Ganesh 1,3, 배성우 1,2,3, Sandeep Kumar Tata 1,2, Tengfang Ling 1, 유병태 1,2* 천연고무는교통기관, 항공, 의료등전세계적으로매우다양한산업에이용되는중요한원자재중하나이다. 그러나이런중요성에도불구하고, 현재천연고무생산은파라고무나무 (Para rubber tree, Hevea brasiliensis) 단한종에만의존하고있다. 따라서천연고무를생산할수있는대체작물에대한연구가필요하다. 러시안민들레 (Russain dandelion, Taraxacum kok-saghyz, 이하 Tks) 는고무나무의천연고무와비슷한수준의품질을가진천연고무를생산하는식물로알려져있다. 다만천연고무의생산비용은고무나무에비해부족하므로이를해결하기위해우리는민들레개체당고무생산력을증가시키는방법을찾아보았다. 식물의바이오매스와뿌리발달에대한메탄올의영향은많은사람들에의해상세히연구되어왔으며, 메탄올을사용하여작물의수확량과생산성을증가시키는데관한몇가지보고가있다. 그래서우리는민들레에메탄올을처리한뒤, 고무생산에어떤변화가일어났는지확인해보았다. 실험결과, 메탄올을처리한민들레와처리하지않은민들레에는육안으로확인가능한분명한차이가있었다. 또, 25, 50, 75% 의메탄올처리시식물개체당고무함량및고무생산량이 25% 처리구에서많이증가하였고 75% 에서는감소, 50% 에서는중간정도의증가를보였다. 25% 메탄올을이용하여옆면살포와뿌리살포를비교하는실험을했을때, 옆면살포에서더큰효과를보였다. 주위환경에노출된밭에서실험을했을때도, 메탄올이처리된식물에서더많은고무생산량을보였다. 실험의결과들을종합하여생각했을때, 메탄올은식물조직내부에탄소공급을증대시키고증가된탄소들은식물체내의 MVA 및 MEP 경로에의해고무생합성을촉진하는것으로보인다. 따라서메탄올을사용하면생산량과함량이증가한다. 다만고농도의메탄올이식물에작용할경우반대의작용을하는것으로보인다. * 교신저자 : Tel , sbryu@kribb.re.kr PF-77 Seung-Ho Paek 1, Ju-Young Choi 1, Bum-Soo Hahn 2, and Seong-Ryong Kim Plant-based molecular farming has advantages in several ways including production cost and safety. However, differences in the N-glycosylation pattern between plant and human proteins act as barriers to the molecular farming using plants. α1,3-fucosyltransferase (OsFucT) is responsible for transferring α 1,3-linked fucose residues to the glycoprotein N-glycan in plants. Osfuct mutant displayed pleiotropic developmental defects such as impaired pollen development and shorter plant height. Transgenic rice expressing a human α 1,6-Fucosyltransferase (HsFucT8) in Osfuct mutant is being analyzed with their N-glycan pattern using LC/MS. For the systematic removal of plant-specific N-glycans, CRISPR/Cas9 vectors were constructed and in vitro cleavage activity of each targets using ribonucleoprotein(rnp) CRISPR/Cas9 were analysed. These studies would facilitate a further understanding of the function of genes mediating N-glycan modification in plants. *Corresponding Author: Tel , sungkim@sogang.ac.kr 259

316 PF-78 Ki-Hwan Kim, Won-Chan Kim * Abiotic stresses such as drought, heat, cold and salinity negatively affect the growth and productivity of crop. In order to cope with abiotic stresses, it necessary to understand the molecular regulatory networks that allow organisms to respond to adverse environmental stresses. Synthetic promoters are commonly used as tools for high level protein production or pathway engineering due to altering upstream regulatory sequence such as transcription factor binding sites. In this study, we screened 5 stress-inducible promoters that are expressed only under stress conditions, thereby founding 40 cis-element in stress-inducible promoters using bioinformatics tool. We also used hybrid-synthetic promoter engineering to construct tightly-controlled, stress-inducible promoters that only express in abiotic stress such as drought. This is achieved by combining cis-elements from the native promoters which are expressed only under abiotic stress. Overall, control of the transcriptional networks is an efficient and useful strategy to be resistant to stress without causing growth delays. Furthermore, this approach enabled us to provide as an enabling tool for future synthetic biology applications that seek to exploit stress-resistance within a plant. *Corresponding Author: Tel , kwc@knu.ac.kr PF-79 Young Jin Park 1, Jeong Su Yoon 1, Seung-A Baek 1, Ju-Seok Seo 2 and Jae Kwang Kim 1* 1 2 The development and cultivation of genetically modified organism (GMO) has been increased continuously over recent years. As cosmetic materials, genetically modified soybeans (GM soybean) event CT-1001 and CT-4025 were developed. These soybeans contain genes that encode epidermal growth factor (EGF) and thioredoxin (TRX) genes for reducing skin wrinkles and improving skin whitening function, respectively. In addition, the phosphinothricin-n-acetyltransferase gene was used as a selectable marker gene for glufosinate tolerance. However, commercialization of these GM soybeans requires the safety assessment. Forty-six key nutrients (proximates, amino acids, fatty acids, isoflavones, vitamins and anti-nutrients) were analyzed in GM soybeans (non-sprayed and sprayed with glufosinate) and non-transgenic soybeans because comparison of composition is an important consideration in the safety assessment of GMO. Soybeans were cultivated in 2017 at two representative regions (Ochang and Jeonju) located in the Republic of Korea. Statistical analysis was performed to assess compositional equivalence between the GM soybean and non-transgenic soybean. The univariate statistical analysis of the linear model showed that most of the analyzed components in the GM soybeans had non-significant differences compared with its non-transgenic soybean. In addition, most nutritional components of GM soybeans fell within the range of values reported for other commercial lines. These comparisons support the conclusion that CT-1001 and CT-4025 are compositionally equivalent to the non-transgenic soybeans. *Corresponding Author: Tel , kjkpj@inu.ac.kr 260

317 PF-80 Sam-Geun Kong 1*, Jae-Woo Han 1, Gyeong-Hoon Lee 1, Koji Okajima 2, Aino Komatsu 3, Fumio Takahashi 4, Takayuki Kohchi 3, Masamitsu Wada Light influences the life of plants not only as an energy source for photosynthesis but also as an environmental signal. Chloroplast photorelocation movement is essential to optimize photosynthetic ability and/or to prevent photodamage under various light conditions. Chloroplasts accumulate at the cell surface under weak light conditions (accumulation response) and move to the anticlinal walls parallel to the direction of incident light under strong light conditions (avoidance response). Chloroplasts attach to the plasma membrane via chloroplast actin (cp-actin) filaments. During the movement, cp-actin filaments are reorganized dynamically according to light intensity and direction. CHLOROPLAST UNUSUAL POSITIONING1 (CHUP1) is a key player not only for chloroplast movement as an actin nucleator but also in chloroplast anchoring by unknown protein-protein interactions. To investigate the molecular mechanism underlying chloroplast anchoring, we carried out yeast two-hybrid assays and identified a CHUP1-INTERACTING PROTEIN 1 (CHIP1) that is a novel plant-specific gene found only in land plant. The interaction between CHUP1 and CHIP1 was further confirmed by in vitro and in vivo binding assays. Furthermore, CHIP1 was phosphorylated in vitro by phot2. Since Arabidopsis has the other two CHIP1-LIKE genes (CHIL1 and CHIL2), any significant phenotypic changes were not observed in the single and double mutants of CHIP1 family. In contrast, chloroplasts in the chip mutants cell aggregated as shown in chup and kac mutant cells in Marchantia polymorpha that has single copies of those genes. These data suggest that chloroplast anchoring is mediated via protein-protein interaction between CHUP1 and CHIP1. *Corresponding Author: Tel , kong@kongju.ac.kr PF-81 Young-Sun Riu 1*, Chang Ho Ahn, Yong Eui Choi, Sam-Geun Kong Light plays pivotal roles not only in plant growth and development as an important environmental signal but also as an energy source for photosynthesis. Chloroplasts change their intracellular positions to optimize photosynthetic efficiency and/or reduce photodamage in response to the intensity and position of light. Chloroplast movement is redundantly mediated by phototropins (phot1 and phot2). The Arabidopsis phot2 mutant is susceptible to photodamage under strong light conditions in which the chloroplasts are accumulated at the cell surface. On the other hand, it is expected that the photosynthetic rate of phot2 mutants could be optimized when light intensity is set at less than a certain critical value to induce photodamage. On the basis of the characteristics of the phot2 mutant, molecular breeding of vegetable crops is challenged to increase plant productivity in the light-controlled plant cultivation systems of plant factory. We chose green leaf lettuce and butterhead lettuce, of which exhibit high chloroplast motility as Arabidopsis does. Two PHOT1 and PHOT2 genes were cloned using PCR, based on the genetic information of Cicerbita plumieri, both of which belong to the same plant family, Compositae. To make lettuce phot2 mutants using CRISPR/Cas9 system, three single-guide RNAs (sgrna) target sites were designed in exons near the beginning of ORFs and were cloned into the Cas9-sgRNA plasmids. Transgenic lettuce plants with antibiotic resistance were obtained using the Agrobacterium-mediated transformation procedure. *Corresponding Author: Tel youngsun432@smail.kongju.ac.kr 261

318 PF-82 Joon-Yung Cha 1*, Masatoshi Nakajima 2, Dae-Jin Yun 3, Woe-Yeon Kim 1* Phytohormone auxin (IAA) is involved in every aspect of plant growth and development and is synthesized from tryptophan. YUCCA (YUC) proteins consisting of 11 homologs in Arabidopsis constitute a family of flavin monooxygenases (FMOs) and participate in indole-3-pyruvic acid (IPA) pathway catalyzing the conversion of IPA to IAA. Previously we reported that Arabidopsis YUC6 exhibits thiol-reductase in vitro and the activity facilitates drought tolerance in planta. Here, we also examined whether overexpression of YUC6 confers other abiotic stress tolerance. First we found that Arabidopsis plants overexpressing YUC6 driven by 35S promoter (YUC6-OX) and activation tagging yuc6-1d mutant exhibit enhanced oxidative stress tolerance with controlled ROS accumulation, suggesting that YUC6 may involve in multiple abiotic stress responses via redox homeostasis. Second, yuc6-1d shows extreme tolerance to nickel-induced heavy metal toxicity. Third, yuc6-1d displays reduced ROS accumulation by cold stress compared to wild-type (WT) plants. To identify whether other phytohormones are also regulated by induced IAA levels through YUC6 overexpression, we analyzed major phytohormones induced by stress condition. While IAA levels increased in YUC6-OX compared to wild type, the levels of jasmonic acid (JA) and salicylic acid (SA) decreased in YUC6-OX plants but abscisic acid (ABA) levels did not change. It suggests that YUC6 overexpression may affect IAA levels only, and be sensitive to biotic stress responses. These data suggest that YUC6 mainly confer the tolerance to abiotic stresses via maintenance of ROS homeostasis. [Supported by a grant from the Next-Generation BioGreen 21 Program (PJ ), Rural Development Administration, Republic of Korea] *Corresponding Author: Joon-Yung Cha, Tel , jycha@gnu.ac.kr/joonyung@hotmail.com PF-83 임명호 1*, 이정동 2, 박태성 1, 조윤성 1, 신공식 차세대바이오그린 21 GM 작물개발사업단의제 1 단계 ( 11~ 14) 및제 2 단계 ( 15~ 17) 의연구성과와사업단을계승한농업생명공학연구단의연구성과물보존과새로운자원으로서의가치부여를통한성과의확대재생산을위한연구사업을추진하고있다. 연구성과물은생명정보 ( 유전자염기서열정보 ), 생명자원 ( 유전자클론, 플라스미드벡터 ), 생물자원 ( 종자, 영양체 ) 으로구분되며이들정보를관리하고보존하며활용하는것이궁극적인목적이다. 주요연구내용은 1) 기존 GM 작물개발사업단연구성과물의유지, 증식, 보존, 2) 미기탁성과물의기탁활성화및연구단신규자원의통합관리, 3) 생명 / 생물정보및자원의데이터베이스구축, 4) 생물자원의중장기보존사업, 5) 생명 / 생물정보및자원의분양과연구소재로의재활용등이다. 구체적실현방안으로농업생명공학연구단홈페이지를통하여기탁된생명 / 생물정보와자원에대한연구이력, 상세정보, 관련참고문헌, 연구결과보고서를 DB 화한후일반 ( 회원 ) 공개하고필요시후속연구에대한추가정보를제공한다. 생물자원의중장기보존은국립농업유전자원센터와업무협약및공조를통하여중장기무상보존할예정이다. 자원 ( 유전자클론, 종자 ) 의분양서비스는연구단이생물자원개발자와수요자를연계하는중개자의역할을수행하는방법으로진행할것이다. 아울러기탁된자원의활력을증진하고특성을검정하기위한소규모의연구사업도병행할계획이다. 이연구사업을통하여연구단성과물의공공소재로의활용을촉진하여관련연구개발사업의연속성유지에기여하고자한다. * 교신저자 : Tel , mlim312@korea.kr 262

319 PF-84 Rupesh Tayade, Krishnanand P Kulkarni, Jeong-Dong Lee Starch is primary insoluble polymeric carbohydrate consisting of glucose, amylopectin and amylose produced mainly by most of higher plants. It also an essential nutritional component of human as well animal diet, also has various food and non-food industrial applications. Legumes which are affordable source of proteins and has potential to produce the starch which has advantage as resistance starch over cereal starch and can be alternative source to meet the increasing world wide demand of starch for various applications. Here we review research in key areas. Frist, we assess the general mechanism of starch synthesis in heterologous organ of legume. Second, we discuss factors influencing of starch digestibility and chemical composition of major legumes. Third, we access the advances at genetic, transcriptomic and metabolomics level in legumes. Finally, we discuss the breeding and biotechnological approaches being used for the improvement in the seed starch content and nutrition quality in legume crops. This may leads to enhance the food and non-food industrial uses of legume starch and give economic benefit to farmer. *Corresponding Author: Jeong-Dong Lee (jdlee@knu.ac.kr) PF-85 Ju Hui Do, Ah Young Kim, Sang Hoon Ma, Hyun Min Kim, Seo Young Park, Thank Dat Mai, Young Hee Joung * Phosphinothricin (PPT) inhibits the synthesis of glutamate synthase and accumulates ammonium in plants and kills plants. The bar gene encodes phosphinothricin acetyltransferase (PAT) and confer resistance to PPT. Thus, the bar gene is used as a selection marker to make herbicide resistant plants. But some dicot plants such as tomato and tobacco, was sensitively affected by phosphinothricin and declined transformation efficiency. We have introduced with two approaches the bar selection system to solve the problems. First was increase bar gene expression level using strong promoter such as enhanced 35S promoter (double 35S), and increase translation efficiency using the modified GUBQ1 promoter (G1-3:tobaint) which was isolated from the gladiolas polyubiquitin gene and replacement 5' intron of the promoter with 5' intron of tobacco polyubiquitin gene. The other was targeting the PAT enzyme to chloroplast using the transit peptide of oxygen evolving protein/rubisco small subunit. This study was carried out by two methods, transient and trangenic plant. The transient was compared with bialaphos treated with leaves, and the transgenic plant was compared with the normal shoot and root expression on medium containing bialaphos. As a result, in tobacco, PAT expression level was highest when using chloroplast transit peptide of rubisco small subunit and transformation yield was best when using modified GUBQ1 promoter. *Corresponding Author: Tel , yhjoung@chonnam.ac.kr 263

320 PF-86 Seo Young Park, Sang Hoon Ma, Ah Young Kim, Hyun Min Kim, Thanh Dat Mai, Ju Hui Do, Chul-Ho Yun, Young Hee Joung * There are various P450s exist in plant and they need electron to do function. Plant NADPH-cytochrome P450 (CPR) is membrane protein that transfer the electron to various plant P450s. Two types of CPR genes (CaCPR1, CaCPR2) were isolated from hot pepper (Capsicum annuum L. cv. Bukang). Quantitative PCR analysis was used for determining CaCPRs mrna expression levels in various hot pepper tissues. The CaCPR1 expression level was gradually increased during fruit ripening. In case of CaCPR2, however, mrna was constitutively expressed in all tissues and expression level was lower than CaCPR1. Under the stress condition, both of CaCPR1 and CaCPR2 were increased. There are two types of CaCPR2 (CaCPR2-f, CaCPR2-d) result from alternative splicing and different mrna expression patterns were observed. CaCPRs were heterogously expressed in Escherichia coli to investigate the enzymatic properties. The enzymatic properties of CaCPRs were determined by characteristic absorption spectrum and catalytic activities measurement, which were assessed using protein and chemical substrates including P450, cytochrome c, ferricyanide and MTT. These results reveal that although the CaCPR2-f is not a major CPR in most tissues in hot pepper, but it could plays important role under the stress condition. *Corresponding Author: Tel , yhjoung@jnu.ac.kr PF-87 Thanh Dat Mai, Ju Hui Do, Seo Young Park, Sang Hoon Ma, Hyun Min Kim, Ah Young Kim, Young Hee Joung * The Cytochrome P450 proteins are the most abundant in number of members at plant comparing the other organisms. As many researches previously reported, plant P450s are necessary for the catalysis of many major reactions, typically in production of primary and secondary metabolites. The functions of these P450s normally involve the biochemical synthesis pathways included of terpenoids, fatty acids, lipids, as well biosynthesis of plant hormones. Especially, the many products which were metabolized by P450s are extremely important for chemical defense mechanism, and those things are their outstanding and noticeable role. Tomato plants also contain numerous CYP genes in their genome, however most of them have unknown function. To study their function, a P450 in CYP736A subfamily, CYP736A72 gene, isolated from tomato (Solanum lycopersicum cv.micro-tom) was selected. To discover the enzyme character of CYP736A72, this gene was heterologously expressed in E. coli, and the protein obtained after induction was used for analytical assays. The initial results show that this enzyme has 7-ethoxycoumarin O-deethylation activity. To identify the function of CYP736A72 in plant, the transgenic lines of tomato and tobacco (Nicotiana tabacum cv.xanthi-nc) which over-expressed CYP736A72 gene were developed and observed. *Corresponding Author: Tel , yhjoung@jnu.ac.kr 264

321 차세대바이오그린21사업 시스템합성농생명공학사업단 2018년 7월 13일(금) 제주 라마다플라자 호텔

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323 OG-01 Beum-Chang Kang 1,2, Je Wook Woo 1, Sang-Tae Kim 1, Minkyung Choi 1, Su-ji Bae 1, Hyeran-Kim 1, Jin-Soo Kim 1,3*, Sang-Gyu Kim 1,4* Cas9 proteins fused with cytidine deaminase can induce C to T nucleotide substitutions at a specific site when directed by guide RNAs. We examined the substitution activity and the substitution range of two base-editing systems: APOBEC1-nCas9 and ncas9-pmcda1 to each other in the protoplasts of Nicotiana tabacum and Brassica napus. We then converted the specific amino acid in the acetolactate synthase gene of N. tabacum to generate herbicide-resistant plants. This study provides guidelines on which a base editor to use and how to adjust the length of a guide RNA for nucleotide substitutions at the desired genomic position in plants. *Corresponding Author: Tel , sgkim1@kaist.ac.kr OG-02 Soon Ju Park 1*#, Sebastian Soyk 2#, Daniel Rodrı guez-leal 2#, Zachary Lippman 2* 1 2 Inflorescence architecture highly influence flower production and crop yields. The inflorescence variations is determined by subtle changes of molecular programs during meristem maturation at the floral transitional stage. Here we introduce two most recent achievements for manipulation of inflorescence architecture especially for improving tomato productivity. First, we achieved a remarkable continuum of inflorescence variants by crossing natural and engineered alleles of inflorescence branching regulators with enhancing dosage sensitivity of the regulators. s/+ and j2 TE ej2 w /+ successfullyallowed breeding of higher yielding genotypes. Second, CRISPR/Cas9 genome editing of S promoter resulted in diverse cis-regulatory alleles that provide beneficial variations in inflorescence branches. Six distinct promoter alleles (S CR-pro ) are revealed a range of inflorescence branching which would lead to breeding process for improving tomato productivity. Therefore, our approaches allow two methods for induction of inflorescence variations and immediate selection for fine-tuning of yield productivity. *Corresponding Author: Tel , sjpark75@wku.ac.kr #. These authors contributed equally 267

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325 차세대바이오그린21사업 농생물게놈활용연구사업단 유전체육종 활성화를 위한 빅데이터 플랫폼 소개 2018년 7월 13일(금) 제주 라마다플라자 호텔

326 - 유전체육종활성화를위한빅데이터플랫폼소개 - 시간 발표 270

327 OH-01 유의수 *, 최아영 작물유전체연구의주요목표중하나는대량의유전체정보에서획득되어진고밀도마커와다중오믹스정보의통합해석의결과를육종프로그램내에적용하여목표형질선발의효율성을강화할수있는형질연관마커의개발및관련유전자의기능적동정에있다. 따라서표준유전체정보가완성된이후국내외적으로벼, 콩, 옥수수등의주요식량작물들은유전체재분석또는 GBS 방법을활용한유전변이확보가가속되고있으며또한다양한형질데이터를확보하기위하여다중오믹스정보 ( 전사체, 표현체, 대사체, 단백질체, 이온체등 ) 의데이터생산에주력하고있다. 이두종류의데이터는형질연관분석을통하여다양하고복잡한양적형질의동정에활용되며유전체기반분자육종을활성화하여종자개발에효율성을증대시킬것으로여겨진다. 데이터베이스구축, 분석도구개발, 가시화및정보의연동등이유전체, 다중오믹스정보를육종에효율적으로활용하기위해반드시필요한요소이지만현재까지각과제별수행결과물중심으로만들어져정보업데이트, 표준화, 연동, 공유등이매우취약한상황이다. 향후데이터를생산, 해석하고이를분자육종에효율적으로적용하기위해서사용자중심의효율적인정보분석플랫폼의개발이반드시필요하다. 이노력의일환으로본과제는사용자편의성이설계된웹인터페이스기반유전변이분석, 마커설계, 집단유전체분석및결과가시화, 형질연관분석및결과가시화, 오믹스데이터연동 SNP 브라우저의개발을통해작물육종에적극활용될수있는오픈소스플랫폼개발을목표로하고있다. 본발표를통해서인터페이스에포함될분자마커개발파이프라인, 형질연관분석및시각화툴등과제수행을통해서구축될도구들을소개하며앞으로의방향을소개하려한다. 이런노력을통해서다양한유전체 / 오믹스정보를분자마커개발, QTL 탐지, 후보유전자동정등분자육종에효율적으로활용할수있게하며, 분자육종의선진화에기여하고자한다. * 교신저자 : Tel , yeisooyu@dnacare.co.kr OH-02 Sung-Hwan Jo *, Junkyoung Choe, Ji-Eun Kim, Bong-Woo Lee, Jeong Hee Lee, Moon Nam It is necessary for molecular breeders to overcome the difficulties in applying abundant genomic information to crop breeding. Candidate orthologs would be discovered more efficiently in less-studied crops if the information gained from studies of related crops were used. We developed a comparative analysis tool and web-based genome viewer to identify orthologous genes based synteny as well as sequence similarity between tomato, pepper and potato. The tool has a step-by-step interface with multiple viewing levels to support the easy and accurate exploration of functional orthologs. Furthermore, it provides access to single nucleotide-polymorphism markers from the massive genetic resource pool in order to accelerate the development of molecular markers for candidate orthologs in the Solanaceae. This tool provides a bridge between genome data and breeding by supporting effective marker development, data utilization and communication. Database URL: *Corresponding Author: Tel , shjo@seeders.co.kr 271

328 OH-03 Jin-Hyun Kim 1, Joo-Seok Park 2 and Hong-Kyu Choi 2* 1 2 Innovation and advancement of NGS technology, in recent years, have played a key role in acceleration of genomic data production and fast accumulation of related omics information. Literally, It is certain that biology is moving towards big data era of omes. Such big data should be useful for molecular breeding and will open a new venue for omics-driven breeding programs. Towards this direction, we are developing and constructing a integrative bioinformatic platform for legume genomics-assisted breeding (named as TGIL: translational genomics interface for the legumes, To build up the platform, a wide array of genome data, including seven fully sequenced legume species (G. max, M. truncatula, L. japonicus, P. vulgaris, C. arietinum, Cajanus cajan and V. radiata) and two non-legume models (A. thaliana and O. sativa), were employed as the fundamental information resources. The platform consists of three major modules, i.e., databases, analytical module and user interface. Including basic genome DB, the database contains gene functional information, orthologous gene DB, transcriptome DB and gene network DB. Analysis platform is equipped with interactive comparative genome analysis module and CSGM (cross-species genic marker) designer program ( as well as other general tools for genome data processing. User interface is being developed with the intention of providing breeder-friendly platform on which they can readily obtain data and/or information associated with traits of interest and design molecular markers. We anticipate that the integration of these bioinformatic modules and tools will contribute to accomplishing a modern concept of genomics-driven breeding, so called reverse breeding or breeding-by-design. *Corresponding Author: Hong-Kyu Choi, hkchoi@dau.ac.kr OH-04 김남신 1,2* 1 2 유전체기반콩연구는 816 점의재배종, 야생종핵심집단의제작, NAM 집단및표현체작성등다양한오믹스를중심으로활발히진행되고있으며특히유전체연구는고밀도 SNP-array 및 NGS 데이터를통하여전장유전체수준에서 GWAS 및집단유전체등다양한오믹스분석방법을적용하고있다. 핵심집단및 NAM 집단의유전자형 - 표현형정보를활용하여상가효과를갖는마커를바탕으로개화기, 지방함량, 단백질함량, 백립중에대하여유전체예측기법으로예측을수행하고있다. 최근에는인공지능기법을통해예측율을향상시키는노력을진행하고있다. 신규핵심집단알고리즘에의해선발된핵심집단을바탕으로전장유전체데이터를생산하였고이를활용하여수천만개이상의방대한량의 SNP, INDEL 등의마커를발굴하였고잠재적으로이를활용하여집단유전체분석기법을도입하여재배화유전자의발굴, 품종간의근연관계등의유추등의오믹스기반연구를수행하고있다. 또한전장유전체데이터는반수체정보를제공해줄수있는리소스로서동위내에존재하는일배체형정보는기존마커의동위마커의검색및활용에사용될수가있다. 이러한모든리소스는콩연구의활성화를위하여현재 k-crop.kr 오픈소스플랫폼을개발하고있으며, 다른작물을포함하여내년부터본격적인서비스를시작할예정이다. * 교신저자 : Tel , n@rna.kr 272

329 Golden Seed Project - 채소종자사업단 / 원예종자사업단 / 식량종자사업단 년 7월 13일(금) 제주 라마다플라자 호텔

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331 OI-01 성동렬 세계인구는계속증가하여 2050 년이면 97 억명이된다는보고가있다. 산업화로인한경작지감소, 집약적인농업의발달로인한농업용수감소, 지구온난화로인한환경의변화, 농업종사자의고령화를고려해보면, 계속증가하는인류를위해식량을공급하는일은매우도전적인일이되리라예측된다. 새로운품종개발및농업생산성을높일수있는기술개발이병행되어야증가하는인구에맞춰식량증산을이뤄나갈수있을것이다. 다행히, 농업산업전반에서다양한기술적혁신이이루어지고있어서당면한인구증가에따른식량증산의문제를어느정도해결할수있으리라기대된다. 기대되는기술혁신을짚어보면, 컴퓨터를이용한산술및예측연산의발달로인한인공지능혹은기계학습분야의발달, 화학적염기분석에서 NGS(Next Generation Sequencing) technology 를기반으로하는유전자염기서열분석의획기적인전환, CRISPR/CAS9 으로본격적으로시작된유전자편집기술등은다양한품종개발을가능케할뿐아니라앞당길수있으리라예상된다. 이러한최신기술동향을엘지화학의그린바이오산업에관한비전과연계시켜고찰해보고자한다. * 교신저자 : dysung@lgchem.com OI-02 Sang Hee Kim 1,2 1 2 Plants are exposed to a wide variety of potential pathogens and have developed a plethora of strategies aimed at protection. Pathogen effectors are injected into host cells where they target components of the host immune system to suppress immunity. Plants, in turn, evolved a defense response using resistance proteins to monitor pathogen effectors. If not properly regulated, immune responses have the potential to be deleterious to the host. For the ultimate goal of engineering durable resistance in crop plants, it is therefore necessary to improve our knowledge of the fundamental principles of plant immune system regulation as well as of resistance protein activation. Our research focuses on developing a novel method to engineer disease resistance in plants that takes advantage of the Arabidopsis resistance gene RPS5, which encodes a CNL resistance protein. RPS5 is normally activated by the proteolytic cleavage of a second host protein, PBS1, by the pathogen-secreted protease AvrPphB. RPS5 senses a conformational change in PBS1 that results from cleavage. Replacement of the AvrPphB-cleavage site within PBS1 with the recognition sequence for AvrRpt2, a different bacterial effector, and with the cleavage site for the NIa protease of Turnip Mosaic Virus activates RPS5 in the presence of the corresponding protease. Significantly, transgenic Arabidopsis expressing PBS1TuMV accumulates less virus. These data suggest that the AvrPphB cleavage site within PBS1 can be substituted with cleavage sites for other pathogen proteases, which will then enable RPS5 to confer disease resistance to new pathogens. The future research aims to understand the molecular mechanisms of plant immune activation and regulation. Ultimately, we plan to translate this foundational knowledge to crops with the goal of engineering durable and broad-spectrum resistance. *Corresponding Author: Tel , sangheekim@gnu.ac.kr 275

332 OI-03 Ujjal Kumar Nath, Song Samnang, Pen Sreyvatey, Jong-In Park, Ill-Sup Nou * Interspecies hybridization is the tool to introduce target traits from one species to another. The family Brassicaceae has two related taxa, Brassica oleracea and B. rapa of important vegetable crops. Due to close genetic relationship, interspecies crosses between them facilitate to transfer beneficial agronomic traits, like glucosinolates (GLS) and β -carotene of secondary metabolites and the resistance genes/qtls of clubroot disease; the devastating one for Brassica vegetables. Cabbage and Chinese cabbage suppose to have GLS and β -carotene (orange colour leaves), respectively. However, no orange colour in cabbage yet to report, aside with the present status of GLS content (ca. 200 μ mol/g) even not satisfactory. In addition, seven CR (clubroot resistance) loci so far reported yet in B. rapa are tried to transfer in cabbage for conferring satisfactory resistance. Therefore, our objectives are to develop cabbage cultivars with orange colour (β -carotene), enhanced GLS status and improved clubroot resistance. Three different interspecies breeding schemes are ongoing; i) high GLS cabbage cultivar, SCNU36 (ca. 200 μ mol/g GLS) was crossed with the twiggy turnip (B. fruticulosa; ca μ mol/g GLS in leaf), ii) a cross was made between orange Chinese cabbage hybrid cultivar β-flash (OR mutant) with cabbage cultivar ASC-82 (white leaves), and iii) clubroot resistance Chinese cabbage lines LCR36 and LCR38 were crossed with two cabbage lines (ASC-82 and Plimio). Embryos were rescued after 20 days of hybridization and placed on MS medium, F1 embryos were confirmed by PCR with A-, F- and C-genome specific markers. The interspecies hybrids were treated with 0.2% colchicine as root-dip method. Amphidiploid plantlets were selected by flow cytometry. BC1F1 and BC2F1 were retrieved by crossing back to SCNU36, ASC-82, and Plimio lines with their amphidiploid F1s. Desired BC2F1 plantlets were selected using HPLC of GLS, PCR amplification of OR and CR allele specific markers, and bioassay for clubroot resistance. Significance changes in GLS profile, pale orange, and clubroot resistance phenotypes of cabbage were detected. We believe that BC2F1 progeny given new types of cabbage lines with enriched secondary metabolites and clubroot resistance. Acknowledgement: Golden Seed Project (Grant No CG100), the Ministry of Agriculture, the Food and Rural Affairs (MAFRA), the Ministry of Oceans and Fisheries (MOF), the Rural Development Administration (RDA) and the Korea Forest Service (KFS), Korea. OI-04 Sathishkumar Natarajan, Ill-Sup Nou * Molecular markers are valuable tools for breeders to accelerate crop improvement. Specifically, High throughput sequencing technologies facilitate the discovery of large-scale variations that have led to direction of next generation plant breeding. Already, a number of bioinformatics methodologies have been developed for molecular markers identification using high-throughput sequencing data. Also, utilizing these technologies, several plants genome sequences and discovery of genetic variations were increased rapidly much faster and cheaper. In this regard, we have customized existing bioinformatics methods/pipelines and successfully implemented in horticultural marker development with aid of sequencing and also public resources. Large number of horticultural samples were collected and sequenced using different sequencing methods (Restriction-site associated DNA sequencing (RAD-seq)), Genotyping by Sequencing (GBS-seq)), Whole genome re-sequencing (WGR)) with specific objectives associated with various diseases. Further, unique bioinformatics pipeline was implemented to handle these large datasets to identify genetic variants such as single nucleotide polymorphisms (SNPs) and insertion and deletions (InDels) in large scale. Thus, the identified potential genetic variants along with available public resources have been correlated together to find out casual genes associated variants, construction of QTL, genetic map, and assisted to marker assisted backcrossing (MABC). In addition, we have developed a simple PCR based pathovar and race specific detection marker for black rot disease and clubroot diseases in Brassica speciesthrough whole genome re-alignment methodologies. (This research was supported by the Golden Seed Project, Grant no CG100). *Corresponding Author: Ill-Sup Nou, Tel , nis@scnu.ac.kr 276

333 OI-05 서상기 중앙아시아는건조한사막성대륙성기후로대부분은수목이잘자라지못하는스텝지역인데천산산맥을중심으로해발 1,500m ~ 2,000m 의고원지대에서감자의주산지가형성되어있다. 겨울철에많이내린눈이고온건조한여름내내작물재배에필요한물을공급함으로써품질이우수한감자를생산하는데좋은조건을가지고있다고볼수있다. 이지역의감자재배면적은약 41 만 ha 로생산량은약 900 만톤에이르고있다. 그중재배면적으로볼때카자흐스탄이약 44% 를차지하고있으며우즈베키스탄과키르기즈스탄이각각 20% 정도를재배하고있다. 구소련시절중아아시아에서는씨감자의생산과유통체계가잘갖추어져있었으나독립후농업체계, 특히씨감자의보급체계가무너졌으며, 중요한식량작물임에도불구하고아직도씨감자의대부분은네델란드나독일에의존하고있는실정이다. GSP 를통해 2014 년부터이지역에서의씨감자사업을위한시장조사, 지역적응성시험을통한우수품종선발, 현지파트너물색등을실시해왔다 년도에는현지법인 SK Seeds' 를설립하였으며금년도알마티인근에씨감자생산농장을확보하여본격적인생산에들어가있다. 중앙아시아는적합한품종개발과씨감자생산및유통체계를확보한다면중앙아시아자체는물론, 전세계감자재배면적의각 30% 와 10% 를차지하는중국과러시아가있어시장의확장성이용이하다고볼수있다. * 교신저자 : Tel , ; sepe33@hanmail.net OI-06 류시환 *, 최재근, 박종열, 남궁민, 박기진, 최준근 옥수수계통육종은우량교잡종을육성하기위한필수단계로서, 99% 이상의순도를가진계통을육종하기위해서는전통적인방법에의해 7 회이상의인공교배 (selfing) 를실시하여야한다. 많은노동력과시간이소요되는전통육종의이러한단점을보완하고자최근선진국을중심으로배가반수체 (Doubled Haploid) 방법에의한계통육종방법이실용화되고있다. 배가반수체방법에의하면 2 3 작기에순도 100% 의계통을육성할수있다. 선진기술인배가반수체기술을도입하여국내의옥수수계통육종에활용하고자강원도농업기술원옥수수연구소에서는국제옥수수 밀연구소 (CIMMYT) 와협력하여배가반수체기술이용에필수적인반수체유기체 (Inducer) 의사용권리를확보하였고, 2014 년부터이기술을국내환경에맞게정착시켜오고있다. 국내에서의옥수수육종은 1 년에 1 작기가일반적이고, 교배시기의잦은강우는화분량이적은배가식물체의교배에부적합하므로시설하우스를설치하여안정성을향상시켰다. 국내에적합한배가반수체기술은유기체와의교배를통한반수체유기및종자선별, 염색체배가및인공교배를통한계통육성, 그리고육성계통의특성평가및종자증식등 3 단계로이루어진다. 찰옥수수및종실용옥수수집단의반수체유기율은각각 % 와 % 였다 년과 2017 년 6 집단으로부터총 431 계통을육성하였다. 육성된계통의표현형적특성평가를실시하여적응성, 도복, 내병성등이우수한종실용 DHF1 및찰옥수수 DHW1 을선발하여국내최초로배가반수체기술에의해육성된계통을국립종자원에출원하였다. 육성된계통은기존의엘리트계통과의교배를통해조합능력및생산력검정을수행하고있으며, 배가반수체기술의국내도입및성공적인정착으로향후옥수수계통및교잡종육성의효율성이크게향상될것으로기대된다. 사사 : 본연구는농촌진흥청차세대바이오그린 21 사업 (PJ ) 및 GSP 사업 (PJ ) 의지원에의해이루어진것임 * 교신저자 : Tel , shr8921@korea.kr 277

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335 산림분야발표 - 산림육종 년 7월 13일(금) 제주 라마다플라자 호텔

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337 OJ-01 S-H Kwon 1, J-M Park 1, Y-G Kim 1, H-I Kang 1, D-B Yeom 1, K-W Kim 1, S-J Na 2 and K-S Kang 1,3* In Korea, people traditionally thought that forests were nature and there was no owner of the forest until Joseon dynasty ( ). The depletion of forests stated to deplete because of the conception. Furthermore, Korean forests had been destroyed much during Japanese colonial ruling ( ) and the Korean War ( ). During the Japanese occupation, a large area of forest was destroyed by over-cutting. In addition, the Korean War lasted for three years. After that, many people went to the forests and cut the trees without any permit from the authority. Thus, almost all mountains except remote areas were denuded. Forest Genetics Research Institute was established in First, the Institute has done much of hybrid breeding such as hybrid pines (Pinus regitaeda) and hybrid poplars (Populus alba x P. glandulosa). Second, introduction breeding has been done. During the Japanese occupation, which was the first phase of introduction, a total of 376 species was introduced from 30 foreign countries and tested adaptation ability at 388 different sites. In the second phase, 415 species from 38 countries were introduced again and eight species were selected and released. In the third phase (1996-present), continuous adaptation test and selection have been doing. Third, plus trees have been selected from wild forests since 1959 and used for establishingseed orchards. Lastly, special purpose tree breeding program has been doing and developed many new varieties of fruits. Recently, molecular breeding and genomics are being applied into conventional works. In my presentation, major achievement and prospect from tree breeding works will be stated and the trend and prospect will also be discussed. *Corresponding Author: Tel , kangks84@snu.ac.kr OJ-02 Eung-Jun Park The aims of this study were 1) to investigate the molecular mechanisms of metabolic-transcriptional regulations in both nut weight (NW) and its sugar contents during entire nut developmental stages, and 2) to select NW-associated RNA-based SNP markers for Korean chestnut trees (Castanea crenata). Omic-datasets were first obtained by metabolic and transcriptomic profiling using two representative genotypes (large vs small nut-bearing individual). Both datasets were analyzed by integrative correlation analysis and further confirmed significant negative correlation between nut weight and sugar contents at Stage III using test accessions (n=30). We also performed RNA sequencing of 42 training accessions. We identified 397,059 high quality SNPs and revealed their transcriptome-wide distribution. Both association and machine learning (ML) studies using 8 different marker sets further showed a clear differentiation between large and small nut-bearing groups. Finally we validated a marker set (21 SNPs, p<10-5 ) with Sanger sequencing, showing that the prediction accuracy by ML matrix ranged 0.70~0.78. Our findings suggest that variation determined using the RNA-seq technology might become an established tool to address future chestnut productivity issues. *Corresponding Author: Tel , pahkej@korea.kr 281

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339 2018년 한국육종학회 학회상 시상 2018년 7월 12일(목) 17:00 제주 라마다프라자 호텔 그랜드볼륨

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341 2018 년한국육종학회학회상시상. - 수상자김용권 ( 신경대학교생명과학과 ) - 선정사유원예종자산업발전을위하여종자개발, 보급및국산화에기여하였고교단에서젊은육종가를배출하는등농가소득증대와인재양성에크게공헌함 - 수상자 진중현 ( 세종대학교바이오산업융합학과 ) - 논문제목 Identification of Heterosis QTLs for Yield and Yield-Related Traits in Indica-Japonica Recombinant Inbred Lines of Rice (Oryza sativa L.) (PBB ) - 수상자 손황배 ( 국립식량과학원고령지농업연구소 ) - 논문제목 콩전장유전체기반의 202개 InDel 마커를활용한한국콩품종의염색체재조합양상구명 (KJBS ) - 수상자 김성용 ( 제주특별자치도농업기술원 ) - 품종명 탐나 [ 감자 ], 제6531호 - 수상자 김세현 ( 국립산림과학원 ) - 품종명 슈퍼자미 [ 벼 ], 제4151호 - 수상자 배석복 ( 농촌진흥청국립식량과학원 ) - 품종명 신팔광 [ 땅콩 ], 제5261호 - 수상자 ( 한국육종학회지 ): 이주경 ( 강원대학교농업생명과학대학 ) - 수상자 (Plant Breeding & Biotechnology): 문중경 ( 국립식량과학원 ) - 수상자 ( 한국육종학회지 ): 박철수 ( 전북대학교농업생명과학대학 ) - 수상자 (Plant Breeding & Biotechnology): 양태진 ( 서울대학교농업생명과학대학 ) 285

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343 한국육종학회연구윤리교육 연구자의연구윤리준수의중요성 연구윤리가제대로확립되었을때비로소연구의진실성이확보된다. 진실성이결여된연구를통해서는진리에도달할수없고, 인류사회발전에기여할수도없다. 진리를추구하는연구자는누구보다도스스로연구윤리를엄격히준수해야한다. 연구윤리를훼손하는행위는학문공동체의성장동력을파괴할뿐아니라국가의위신과장래까지도훼손할수있기때문이다 - 출처 : 연구윤리확립추진위원회, 연구윤리확립을위한권고문 ( ) 가. 출처를표시하지않은경우 유형 1: 타인의저작물에있는독창적인아이디어나사고구조 ( 생각의프레임 ) 를출처를밝히지않고자신의것인것처럼활용하는경우 유형 2: 일반적지식이아닌타인의독창적인개념 ( 용어 ), 어휘 ( 구 ), 문장, 단락, 그림, 표, 사진, 데이터등을출처를밝히지않고활용한경우 유형 3: 타인이쓴글을그대로쓰지않고저자가말바꿔쓰기 (paraphrasing) 또는요약 (summarizing) 했지만, 출처를표시하지않은경우나. 출처를표시했지만부적절하게표시한경우 유형 1: 활용한타인의저작물에대해출처를표시했지만직접인용하면서도인용부호 ( ) 없이그대로사용한경우 유형 2: 2 차문헌표절 저자가외국인이쓴 1 차문헌을직접보고그대로번역하거나말바꿔쓰기또는요약을하지않고 2 차문헌에인용된내용을그대로쓸때, 재인용표시를해야함에도직접원문을본것처럼 1 차문헌을출처표시한경우 유형 3: 인용된부분에출처를표시했지만본인의것이라고인정할수없을정도로너무많은아이디어나어구및문장을가져온경우 유형 1 : 출처표시를하지않은중복게재 ( 자신의이전저작물의일부나상당부분이이후의저작물에서활용되고있지만출처를표시하지않아, 마치새로운것을처음발표하는것으로오해케하는경우 유형 2: 출처표시가정확하지않은중복게재 ( 자신의이전저작물을여기저기서가져와활용하면서도어느일부분만출처를표시하고나머지부분은표시하지않는경우 ) 유형 1 : 저자로서정당한자격을갖춘사람에게저자자격을부여하지않거나, 저자로서정당한자격을갖추지못한사람에게저자자격을부여한경우 유형 2 : 연구내용중일부를연구자가아닌다른사람에게 2 차용역을주고그결과를최종보고서에포함시킬때, 저자표시를적절하게하지않은경우 유형 1 : 본문에서활용한저작물에대한출처의누락혹은부정확한표기 - 출처 : 경제 인문사회연구회, 연구윤리사례집 ( ) 287

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347 Name Page Name Page Name Page A Reum Han 172 A-Ram Kim 111 Abinaya Manivannan 56 Adeva Cheryl 212 Adrian F. Powell 3 Ah Young Kim 73, 74, 104 Ah young Kim 72 Aino Komatsu 261 Ancheol Chang 70, 139 Andre Silvanovich 132 Anh Quynh Le 123 Anil Kumar N.C 16, 239 Ankita Pandey 190, 196 Annapurna Sahoo 235 Annhee Im 186 Ara Cho 232 Awraris Derbie Assefa 178 B. Kim 138 Baek Hie Nahm 43, 144 Beom Gi Kim 109, 194 Beom-Gi Kim 110 Beom-Kyu Kang 94, 156 Beom-Young Son 114 Beum-Chang Kang 267 Bo Mi Nam 141 Bo Ram Lee 239 Bo-Keun Ha 33, 101, 107, 128, 141, 142, 143, 144, 149 Bo-Kyeong Kim 84 Bo-Mi Lee 145 Bo-Ra Park 207 Bo-ram Choi 203 Bon-Cheol Koo 137, 138, 187 Bong-Woo Lee 271 Bora Park 44, 158 Boram Beak 216 Boram Choi 161 Boseul Park 96 Boyoung Kim 13, 190 Bradley Comstock 132 Bum-Soo Hahn 25, 222, 259 Buung Choi 122 Byeogil Je 90 Byeong Cheol Moon 169 Byeong Hoon Kim 242 Byeong-Chul Moon 83 Byeong-Churl Moon 122 Byeongrim Go 244 Byong Gon Choi 154 Byoung-Cheorl Kang 13, 32, 67, 92, 169, 175, 209 Byoung-Doo Lee 227, 228 Byoung-Ju Yun 225 Byoung-Ohg Ahn 71 Byung Sup Kim 90 Byung-Moo Lee 108 Byung-Ohg Ahn 173 Byung-Wook Yun 14 C. Kim 138 Cai-Zhong Jiang 32 Caren Chang 32 Carl Garnaat 132 Cha Young Kim 167 Chae Woo Lim 184, 185, 221 Chan Seop Ko 122 Chan-Ho Kang 115 Chan-Kyu Lim 157 Chang Ho Ahn 261 Chang Hyun Jin 141 Chang Kil Kim 230 Chang Mi Kim 229 Chang Pyo Hong 19, 159, 161 Chang Sook Ahn 191 Chang Sun Sin 158 Chang-deok Han 196 Chang-Duck Koo 50 Chang-Ho Ahn 28 Chang-Ho Eun 171 Chang-Ho Kim 112 Chang-Hwan Park 94, 156 Chang-Hyu Bae 150 Chang-Kug Kim 159 Chang-Min Lee 11, 51 Chang-Sik Oh 13 Changsoo Kim 19, 23, 133 Chanseok Shin 25, 185 Charlotte Bureau 189 Cheol Choi 99, 168, 172 Cheol Seong Jang 113, 167, 210, 212 Cheol Won Lee 87 Cheol-Won Yun 257 Cheryl Adeva 19, 121 Chi-hwan Kim 164 Chi-Yeol Kim 100 Chi Eun Hong 59 Cho-Yil Cho 173 Chon-Sik Kang 23, 24 Chong Chen 168 Choon-Ki Lee 34 Choon-Song Kim 11, 51 Choong-Ill Cheon 199 Choonkyun Jung 203 Choonseok Lee 197 Christophe Périn 189 Chuan-Jie Zhang 174 Chul Soo Park 23, 24, 215 Chul-Ho Yun 104 Chul-ho Yun 72 Chun-Hwan Kim 157 Chun-Song Kim 84 Chungmo-Park 218 Colton Kessenich 132 Cuc Thi Nguyen 119 Cécile Segonzac 190 D-B Yeom 281 Da Som Lee 126 Da-Eun Im 75, 105 Da-Hye Kim 44, 131, 158, 207 Dae Gyeom Oh 113 Dae Hyun Park 26 Dae Yeon Kim 166 Dae Yoen Kim 103 Dae-Jin Yun 119, 262 Dae-woo Lee 100 Daewoong Lee 151 Daniel Rodrı guez-leal 267 Danim Jo

348 Name Page Name Page Name Page Dany Resolus 226 Daoweng Wang 4 Daun Kim 90 David Twell 258 Deok-Ryeol Lee 154 Do Hyeon Gwon 135 Do Won Yun 70, 247 Do Yeon Kwak 66, 126 Do Yoon Hyun 125, 166, 176 Do yoon Hyun 137 Do-Soon Kim 174, 213, 214 Do-Sun Kim 56, 175 Do-Wan Kim 173 Do-Yeon Kwak 66, 94, 156, 170 Do-Young Bae 117 Doh-Won Yun 139, 228 Doil Choi 13, 96, 187 Dong Hwan Park 239 Dong Hyun Kang 132 Dong Hyun Kim 158 Dong Jin Lee 159 Dong Soo Park 133 Dong Woo Lee 142 Dong-Eun Kim 144 Dong-Gun Kim 141, 144, 149, 150 Dong-Hoon Jeong 243 Dong-Hyuk Lee 99 Dong-Jin Shin 36 Dong-Jun Lee 173 Dong-Keun Lee 229 Dong-Kwan Kim 107 Dong-Seon Kim 216 Dong-Soo Park 55, 91, 117 Dong-yong Kim 105 Dong-Yup Lee 5 Donggyu Lee 214 Donghwan Shim 35 Dongjin Shin 117 Dongryung Lee 93 DongSub Kim 229 Doo-Gyung Moon 157 Dool Yi Kim 106 Dool-Yi Kim 159 Doolyi Kim 109 Doreen Ware 232 Du-Hwa Lee 191 Duk-Ju Hwang 104 Ee-Youb Kim 143 Elnura Torutaeva 68 Erin Bell 139 Eui-Shik Rha 228 Eun Gyeong Kim 224 Eun Hee Kim 104 Eun Ji Lee 133 Eun Ju Cheong 60 Eun Ju Lee 37 Eun Jung Suh 27 Eun Young Oh 66 Eun-ha Park 242 Eun-jin Cho 23, 215 Eun-Jo Shim 89 Eun-Ju Byeon 28 Eun-Ju Song 154 Eun-Jung Kim 186 Eun-Kyoung Kim 149 Eun-Seok Oh 134, 140 Eun-Su Lee 56 Eun-Yeong Sim 34 Eun-Young Oh 66, 156 Eunbyeol Koh 93 Eung-Jun Park 18, 281 Eungyeong Lee 16 Eunhye Goo 253 Eunjeong Choi 54 Eunkyoung Jun 164 Eunsook Park 187 Eunyoung Oh 67, 170 Eunyoung Seo 13 Franklin Hinosa Mancia 131 Franz M. Nogoy 14, 92, 145, 146 Fumio Takahashi 261 Ga-hee Kim 46 Ga-Hyeon Kim 228 Ga-young Lee 72 Gagan Kumar Panigrahi 235 Ganchudur Tsetsegmaa 54 Gang Seob Lee 194 Gang-Seob Lee 28, 43, 70, 81, 83, 111, 139, 144 Gayoung Jung 196 Geum Sol Kim 167 Geung-Joo Lee 69 Geupil Jang 189, 230 Gi Hoon Kim 99, 172 Gi hoon Kim 168 Gi-An Lee 45, 46, 47, 48 Gileung Lee 36 Green Jhang 118, 148 Gregory B. Martin 3 Gregory B. Tilton 139 Gun-Hyoung Cho 206 Gun-Mi Lee 11, 51 Gyeong-Hoon Lee 261 Gyeong-Jin Kim 122 Gynheung An 16, 17, 152, 189 Gyoungju Nah 118, 148, 149, 164 Gyu Tae Park 119, 163, 165 Gyu Taek Cho 137, 166 Gyu-Taek Cho 45, 46, 47, 48 Gyutae Kim 107 H-I Kang 281 Ha-Cheol Hong 34 Ha-Yeon Yoon 103 Hae Eun Kim 151 Hae Rim Park 148 Hae-Ik Rhee 26 Hae-Rim Park 174 Hae-Ryong Jeong 130 Hak Soo Seo 163, 165, 195, 200 Hakgi Jang 97 Han-Jig Jo 143 Hana Jeong 118 Hana Yu 35 Hanhong Bae 241, 254 Hanul Kim 186 Hay Ju Han 194 Hayoung Moon 190, 196 Hea-Young Lee 13, 169 Heayoung Lee

349 Name Page Name Page Name Page Hee Kyong Kim 158 Hee Kyung Kim 202 Hee Rang Yun 151 Hee-Jeong Jung 90 Hee-Jong Koh 3, 36, 83, 93, 153, 197 Hee-Seung Choi 76 Heejin Kim 90, 93, 94, 204 Heng Wang 75, 105 Ho Bang Kim 192, 193, 206 Ho Jun Joh 20, 124, 199 Ho-sun Lee 178 Hocheol Ko 137, 166 Hoduck Kang 54 Hojin Ryu 19, 50, 108 Hokeun Sun 33 Hokyoung Son 89 Hong Min Koh 101 Hong-Gyu Kang 118 Hong-Il Ahn 71, 161 Hong-Il Choi 71, 141, 166 Hong-Kyu Choi 256, 272 Hong-Seob Yu 20, 199 Hong-Sig Kim 129 Hong-Sik Kim 94, 156 Hongseock Lee 16 Hongsik Won 196 Honour C. McCann 196 Hoseong Choi 188 Hwa Lee 60 Hwa-Yong Lee 50, 57 Hwa-Young An 149 Hwan Hee Bae 114 Hwang-Bae Sohn 137, 138, 187 Hwang-weon Jeong 246 Hwayong Lee 19 Hwi-Young Park 234, 247, 249, 250 Hyang Sook Chun 148, 149, 164 Hye In Jeong 229 Hye Jeong Cho 172 Hye Jeong Kim 119, 167, 253 Hye Jin Seo 131 Hye Jung Hyun 132 Hye Seon Kim 104 Hye-Eun Lee 56 Hye-Jin Yoon 27, 127 Hye-Jung Lee 192 Hye-Kyoung Ju 241, 254 Hye-Kyung Son 153 Hye-min Lim 111 Hye-Won Kim 89 Hyeah Yu 214 Hyejin An 57 Hyemin Kim 108 Hyemin Lim 54, 86 Hyenso Ji 16 Hyeon-Jin Sun 118 Hyeon-So Ji 83 Hyeona Hwang 108 Hyeonah Shim 199 Hyeong Cheol Park 106 Hyeong-Jun Park 129 Hyeong-Soo Park 28 Hyeonso Ji 28, 63, 100, 206 Hyeran-Kim 267 Hyeree Kim 225 Hyo Bin Park 131 Hyo Ja Oh 63, 100, 206 Hyo Jin Park 258 Hyo-Jin Park 258 Hyo-Ju Lee 158, 202 Hyo-Yeon Lee 118 Hyochul Kim 108 Hyoseob Seo 227 Hyoshin Lee 18 Hyoun-Sub Lim 241, 254 Hyun A Jang 103 Hyun Min Kim 73, 74, 104 Hyun min Kim 72 Hyun Namgung 81, 83 Hyun Oh Lee 169 Hyun Suk Cho 119, 167, 253 Hyun Uk Kim 253 Hyun Wook Shin 172 Hyun Young Sing 230 Hyun-Ah Han 154 Hyun-Jin Jung 129 Hyun-Jin Koo 177 Hyun-Ju Kang 63, 100, 206 Hyun-Jung Kim 89 Hyun-Min Cho 76 Hyun-Seung Park 20, 199 Hyun-Sook Lee 17, 19, 121, 123 Hyun-Sook Pai 191 Hyun-Su Park 11, 51, 84 Hyun-Suk Cho 234, 247, 249, 250 Hyun-Tae Kim 94, 156 Hyung Jin Lee 13 Hyung-jin Baek 124 Hyung-Keun Ku 242, 243 Hyung-Taeg Cho 76 Hyung-Woo Jeon 18 Hyunhee Joo 184, 185 Hyunjo Koo 177 Hyunju Ha 97 Hyunseok Lee 54, 86 Ick Hyun Jo 59 Ik Young Choi 154 Ik-Young Choi 26, 35, 60, 204 Il Hwan Lee 35 Il-Pyung Ahn 83, 104 Il-Sup Kim 123 Ilha Lee 198, 202 Ill-Min Chung 83 Ill-Sup Nou 276 Ill-Sup Noua 90 Illsup Nou 5 Im Ji Hwan 36 In Hye Lee 251 In Sun Hwang 13 In Sun Yoon 109, 110, 194, 206 In-Gang Shin 161 In-Jung Kim 171 In-Jung Lee 223 In-Sok Lee 115 In-Sun Yoon 109 In-Taek Hwang 90, 94, 204 Inchan Choi 16, 63, 100, 206, 246 Ingyu Hwang 253 Inhwan Hwang 255 Inkyu Park 169 Irisappan Ganesh 259 J-M Park 281 Ja-Hong Lee 75, 105 Jae Bok Heo 151, 168 Jae Buhm Chun

350 Name Page Name Page Name Page Jae Eun Lee 178 Jae Kwang Kim 242, 243, 260 Jae Seong Kim 119 Jae Suk Jan 238 Jae Sung Shim 229 Jae Uk Lee 158 Jae Wan Park 71 Jae Yong Han 119, 167, 253 Jae Yoon Kim 108, 112 Jae Young Choi 239 Jae Yun Lim 253 Jae-Eun Oh 145 Jae-eun Oh 170 Jae-Heung Ko 18 Jae-Hoon Moon 15 Jae-Hyeon Oh 173 Jae-Hyuk Han 15, 208 Jae-In Chun 90, 94 Jae-Keun Choi 26 Jae-Woo Han 261 Jae-Young Song 145 Jaebok Lee 192, 193 Jaebuhm Chun 159 Jaeeun Jung 236 JaeEun Lee 214 Jaeeun Song 25, 222 Jaeho Kim 203 Jaehyeon Jeon 124 Jaeil Kim 186 Jaesung Nam 165 Jagadeesh Sundaramoorthy 119, 163, 165 Jaihyunk Ryu 142, 143, 149 Jan Dvorak 4 Jang Ryol Liu 229 Jang-Hwan Yu 129 Jang-Kyun Seo 12, 203 Jayern Lee 69 Je Dong Kim 135 Je Min Lee 13 Je Wook Woo 267 Je-Hun Moon 121, 208 Jee Hye Kim 151 Jee Yeon Ko 126 Jee Young Park 20, 124 Jee-Su Kang 80, 83 Jeffery L. Caplan 187 Jelli Venkatesh 13, 209 Jemin Kim 25, 185 Jeom-Ho Lee 84 Jeom-Sig Lee 34 Jeong Hee Lee 271 Jeong Heo Kim 258 Jeong Ho Baek 16 Jeong Sheop Shin 234 Jeong Su Yoon 260 Jeong Sun Park 158 Jeong-Dong Lee 116, 119, 128, 165, 223, 263 Jeong-Hee Kim 99 Jeong-Hee Lee 142, 143, 145, 161 Jeong-Hwan Mun 232 Jeong-Hyun Lim 227 Jeong-Hyun Seo 94, 156 Jeong-Kwon Nam 11, 51 Jeong-Man Kim 115 JeongHo BAEK 246 Jeongho Baek 63, 100, 206 Jeonghwan Seo 36 Ji E Liang 217 Ji Hyeon Yu 202 Ji Seon Choi 58 Ji Seon Song 109 Ji Yoon Lee 133 Ji Yul Lee 168 Ji Yun Ko 245 Ji-Eun Kim 271 Ji-Hye Hwang 81, 83 Ji-Hyock Ryu 122 Ji-Hyun Yoon 122 Ji-Min Kim 101, 205 Ji-Min Yoo 81, 83, 85 Ji-Nam Kang 71 Ji-Yoon Lee 55, 91 Ji-Young Lee 236 Ji-Yun Lee 84 Jieun Kwak 34 Jihee Park 27, 246 Jiheun Noh 99 Jihye Park 186 Jimin Lee 203 Jin A Kim 105, 178 Jin Joo Choi 229 Jin seok Moon 197 Jin Seok Yoon 213 Jin Sol Park 119, 167, 253 Jin Sun Kim 155, 183 Jin Won Lee 126 Jin-Ae Kim 110 Jin-Baek Kim 71, 122, 141, 144, 149, 150 Jin-Beak Kim 166 Jin-Ho Kang 90, 93, 94, 204 Jin-Hong Kim 113 Jin-hyuk Kim 149 Jin-Hyun Kim 256, 272 Jin-Ju Kim 123 Jin-Kee Jung 89 Jin-Kyeong Cha 36 Jin-Kyoung Kwon 92 Jin-Kyu Woo 206 Jin-Kyung Kwon 13, 67, 169, 209 Jin-Seok Lee 114 Jin-Seoung Cho 18 Jin-Soo Kim 267 Jin-Suk Kim 228 Jin-Won Kim 214 Jina Bae 198 Jing Wen Ma 217 Jinhee Kim 56, 175 Jinho Heo 101 Jinjoong Kim 35 Jinmi Yoon 189 Jinseul Kyung 202 Jinsu Gil 58, 192, 193 JinSu Lee 193 Jinuk Bhak 142 Jinwon Lee 152 Jinwoo Kim 253 Jiseok Beak 236 Jiwon Han 54 Jiwon Jung 214 Jiwon Kim 214 Jiyeon Jeong 206 Jiyeong Jung 101 Jiyoung Kim 139 Jonathan LaMantia 9 Jong Hee Kim 158 Jong Hee Lee 133 Jong Hoon Kim 239 Jong Hyang Bae 209 Jong Min Ko 133 Jong Tae Song 119, 163, 165, 200 Jong Yeol Park

351 Name Page Name Page Name Page Jong-Chan Park 16 Jong-Hee Lee 55, 91, 117 Jong-Il Chung 140, 141 Jong-In Park 276 Jong-Joo Cheong 144 Jong-Min Jeong 84 Jong-Min Ko 36, 55, 91, 117 Jong-Seong Jeon 100, 208 Jong-Tae Song 116, 128 Jong-Wook Chung 57, 58, 59 Jong-Yeol Lee 4, 44, 131, 155, 158, 183, 197, 207 Jongchan Woo 187 Jonghyun Kwon 132 JongSung Lim 148 Jongwook Ahn 67, 92 Joo Ohk Sohn 197 Joo Yeol Kim 178 Joo-hyun Lee 83 Joo-Seok Park 256, 272 Joo-Won Kang 55, 91 Joohee Choi 238 Joohyun Lee 114 Joon Ki Hong 27 Joon ki Kim 192 Joon-Soo Sim 25 Joon-Woo Ahn 149, 150, 166 Joon-Yung Cha 262 Joong Hyoun Chin 15, 208 Joong-Hyoun Chin 83 Joonyup Kim 32 Jooyeon Bae 238 Joung Sug Kim 43, 144 Joung Suk Choi 224 Joung-Ho Lee 67, 92 Ju Hee Jung 194 Ju Hee Kim 210 Ju Hui Do 73, 74, 104 Ju hui Do 72 Ju Hun Noh 135 Ju Kyong Lee 26, 153, 204 Ju Seok Lee 101, 205 Ju Won Kang 133 Ju-Hee Rhee 178 Ju-Kon Kim 229, 238, 239 Ju-Pyo Hong 169 Ju-pyo Hong 175 Ju-Seok Seo 260 Ju-Young Choi 88, 92, 129, 259 Ju-Young Yang 14, 146 Juhyun Im 205 Juhyun Kim 186 Jun Hee Jung 89 Jun Hyeon Cho 133 Jun Oh 63, 100, 206 Jun Young Choi 37 Jun-Cheol Moon 108 Jun-Hyeon Cho 55, 91, 117 Jun-Hyun Cho 19, 84 Jun-Hyun Park 140, 141 Jun-Myeong Lee 105 Jun-Won Kang 54, 86 Jundae Lee 161 Junesung Lee 96 Junewoo Lee 32 Jung Eun Hwang 106 Jung Heo 174, 209 Jung In Kim 66, 170 Jung Min Kim 141, 144, 149, 150 Jung Min Yoon 188 Jung Suk Kim 238 Jung Sun Kim 161 Jung Yeon Han 247 Jung-Eun Kim 89 Jung-In Kim 66 Jung-Kyu Kim 241, 254 Jung-Kyung Moon 33, 81, 83, 101, 159 Jung-Min Park 23, 24 Jung-Ro Lee 45, 46, 47, 48 Jung-Sook Sung 178 Jung-Tae Kim 114 Jung-Woo Lee 121 Jung-yoon Yi 124 JungIn Kim 67 Jungmin Ha 69, 89, 120, 130 Jungrye Nam 79, 80, 81, 82, 83 Junho Lee 255 Junki Lee 148, 149, 164 Junkyoung Choe 170, 271 Junsu Ko 19 Junsub Lim 184 K-S Kang 281 K-W Kim 281 Ka-Youn Lee 69 Kang-Heum Cho 130 Kang-Seop Lee 143 Kee Hoon Sohn 190, 196 Keon-Il Kim 95 Kesavan Markkandan 19, 142, 161 Keunpyo Lee 71 Ki Hong Nam 158 Ki Hun Shin 235 Ki Jin Park 26 Ki-Byung Lim 131 Ki-Deuk Bae 61 Ki-Hong Jung 16, 83, 117, 189, 242 Ki-Hwan Kim 260 Ki-Hyun Kim 88 Ki-Won Oh 66, 145 Kieu Thi Xuan Vo 100 Kihwan Song 124 Kisung Kwon 148 Kitae Song 108 Kiyoon Kang 15, 198 Koeun Han 13, 175 Koji Okajima 261 Kong-Sik Shin 134 Kook-Hyung Kim 12, 188 Kook-HyungKim 218 Krishnanand P Kulkarni 263 Krishnanand P. Kulkarni 66 Kristin Widyasari 12, 218 Ku-Hyun Kwon 130, 192 Kunyan Zou 151 Kwan Jeong Song 206 Kwanbeen Park 97 Kwangsoo Cho 196 Kwon Kyoo Kang 158, 202, 245, 251 Kwon-Kyoo Kang 14, 92, 145, 146, 192 Ky Young Park 189 Kyeong Do Min 112 Kyeong Hoon Kim 122 Kyeong Min Kim 133 Kyeong-Hoon Kim 36 Kyeong-Jae Heo 203 Kyeong-Min Kim 36 Kyeong-Seong Cheon 28, 161 Kyo-Sang Lee 103 Kyong Cheul Park 35, 153,

352 Name Page Name Page Name Page Kyong Mi Jun 43, 144 Kyong Sil Lee 104 Kyong-Cheul Park 26, 204 Kyong Hwan Bang 59 Kyoungmin Lee 192, 193 Kyoungwon Cho 194 Kyu Hang Kyung 229 Kyu Jin Sa 26, 204 Kyu-Chan Shim 19, 121, 123 Kyu-Won Kim 78, 79, 80, 81, 82, 83, 122, 127, 205 Kyuha Choi 186 Kyujung Van 9 Kyung Eun Lee 177 Kyung Hae-Kim 205 Kyung Hwa Kim 106 Kyung Hye Kim 101 Kyung Jun Lee 45, 46, 47, 48, 58 Kyung Man Kim 235 Kyung-Hee Kim 108 Kyung-Ho Ma 45, 46, 47, 48 Kyung-Hwan Kim 16, 63, 100, 206 Kyung-Min Kim 135, 136, 224, 225, 226 Kyung-Won Kang 142 Kyungmun Kim 101 Lae-Hyeon Cho 17, 189 Le Duc Thao 12 Le Van Trang 14, 146 Leah K. McHale 9 Lee Ye Jun 131 Leitao Pedro Isabel 136 Leslie L. Domier 241, 254 Lin Cheng 79, 83 Lingli Dong 4 Lu Lu 132 Luhua Tu 69 Luong Ngoc Ha 19, 121 Luong Nogc Ha 212 M. A. Rouf Mian 9 Mai Thanh Dat 72 Man Soo Choi 33, 101, 106, 159 Man-Ho Oh 134, 140 Man-Kee Baek 11, 51, 109 Man-Ki Baek 84 Man-Sun Kim 132 Man-Sung Yang 37 Mansik Jeon 225 Marianne Malven 132 Maricar Ferrer Flores 70 Marjohn Nino 14, 92, 145, 146 Mark L. Tucker 32 Masamitsu Wada 261 Masatoshi Nakajima 262 Maxim Prokchorchik 196 May Moe Oo 103 Mazen Alazem 218 Md Reyazul Islam 255 Me-Sun Kim 14, 92, 145, 146 Mi Hyun Cho 109 Mi Jeong Jeong 178 Mi Jin Kim 37 Mi na Jin 106 Mi Young Kang 217 Mi-Jeong Jeong 105 Mi-Ra Yoon 34 Mi-Reu Kim 103 Mi-Su An 89 Mi-Young Park 118 Mia Park 139 Michael Kovach 192 Mijeong Kim 114 Mikyong Kang 197 Mikyung Sung 9 Min Gu Park 239 Min Hee Ju 131 Min Hee Kim 131 Min Jeong Hong 71, 103, 122, 141, 144, 149, 150, 166 Min Ju Kim 101 Min Jung 37 Min Jung Kim 177 Min Jung Yook 174, 213, 214 Min Kang 111 Min Kyoung You 242 Min-Gyun Jeong 256 Min-Ha Kim 18 Min-Ki Lee 155 Min-Kyu Lee 141, 144, 149, 150 Min-Sung Kang 174, 209 Min-Young Kang 67 Mina Jin 159 Mingcheng Luo 4 Mingmin Zhao 258 Mingyun Jeong 256 Minhee Kang 253 Minho Na 60 Minjeong Park 175 Minjin Kim 244 Minjung Kim 149 Minkyung Choi 267 Minsu Kim 128 Minsu Park 25, 185 Moon Nam 145, 271 Moon Young Kim 89, 130 Moon-Soo Soh 126 Muhammad Farooq 225 Muhammad Irfan Siddique 13 Muhammad Rauf 125, 176 Mun-seop Yoon 124 Munsup Yoon 166 Myeong-Hyun Min 83, 127 Myeong-Jin Kang 127 MyeongEun Choe 126 Myeongjune Jeon 202 Myoung Hee Lee 66, 67, 170 Myoung-Hee Lee 145, 161 Myoung-Jae Shin 45, 46, 47, 48 Myung Hee Nam 194 Myung Hwan Shin 106 Myung Ki Min 110 Myung-Chul Lee 125, 166, 176 Myung-chul Lee 137 Myung-Hee Lee 66 Myung-Ho Lim 134 Myung-Hwi Kim 203 Na Young Ro 169 Na-Hyun Shin 15, 70, 208 Na-Rae Jeong 90, 93, 94, 204 Na-Young Ro 178 Nam-Chon Paek 15, 83, 198, 227, 228 Nam-In Hyung 28,

353 Name Page Name Page Name Page Nam-Soo Jwa 188 Namgeol Kim 34 Namhee Jeong 159 Namjin Koo 96 Namjoo Kang 214 Namshin Kim 101 Naxin Huo 4 Nayoung Kim 97 Nayoung Kwon 151 Neha Roy Samir 35 Niaz Mohammad Rahimi 116, 128 Nikita Bhatnagar 110 Ning Zhang 3 Nozomu Sakurai 221 Nuri Oh 203 Nyunhee Kim 16 Oksoo Han 194 On-Sook Hur 166, 178 Onsook Hur 137 Paulina Calderon Flores 210, 211 Pen Sreyvatey 276 Phanna Phat 47 Phu Tri Tran 218 Phu-Tri Tran 12 Pil Joong Chung 203, 238 Pureum Noh 169 Qing Tian 132 Ra Mi Woo 239 Rahmatullah Jan 136 Rahul Vasudeo Ramekar 153, 154 Randall Nelson 9 Robyn Roberts 3 Romika Chandra 54 Rongchen Wang 189 Ronghui Yang 32 Rungnapa Phitaktansakul 80, 83 Rupesh Tayade 128, 263 Rusell Reinke 98 Ryulyi Kang 107 S-H Kwon 281 S-J Na 281 S. Kim 138 Sae Hyun Lee 20, 179, 199 Saes-Beul Lee 55 Saima Akhter 68 Sais-Beul Lee 91 Sam-Geun Kong 261 Samantha Mainiero 3 Saminathan Subburaj 69 San Mar Lar 75, 105 Sandeep Kumar Tata 259 Sang Cheul Lee 212 Sang Chul Choi 133 Sang Dae Yun 126 Sang Gon Kim 114 Sang Gu Kang 177 Sang Hee Kim 275 Sang Hoon Kim 71, 142, 166 Sang Hoon Ma 73, 74, 104 Sang hoon Ma 72 Sang Hyeon Kim 101 Sang Hyon Kim 235 Sang Ik Park 192, 193 Sang Ik Song 144 Sang Ju Lee 258 Sang Ryeol Park 104, 246 Sang Yong Park 112 Sang-Beom Lee 83, 122 Sang-Bong Choi 232 Sang-Choon Lee 124 Sang-Gu Lee 234, 247, 249, 250 Sang-Gyu Kim 267 Sang-Heon Choi 129, 192 Sang-Heon Jin 105 Sang-Ho Chu 78, 80, 81, 83, 85, 122, 127 Sang-Hyeon Jin 75 Sang-Ji Lee 227 Sang-Keun Oh 103 Sang-Kyu Lee 208 Sang-Nag Ahn 17, 19, 121, 123 Sang-Ouk Shin 94, 156 Sang-Tae Kim 267 Sang-Won Lee 16 Sang-Won Park 83, 122 Sang-Woo Choi 140, 141 Sang-Yoon Shin 25 Sanghee Um 149 Sangho Lee 236 Sangkee Rhee 186 SangKyu Park 131, 158 Sangkyu Park 44, 207 Sangrea Shim 69, 130 Sangsu Bae 69, 202 Sangyool Lee 189, 230 Sathishkumar Natarajan 276 Savithramma P. Dinesh-Kumar 187 Se Nam Lee 229 Sea-Hee Han 47 Sebastian Soyk 267 Sebastin Raveendar 45, 46, 47, 48, 57, 58, 59 Sejong Oh 125, 137, 166, 176 Seo Young Park 73, 74, 104 Seo young Park 72 Seok Hee Lee 239 Seok-Hyun Choi 100, 208 Seokhyung Lee 165 Seon-Hwa Bae 161 Seon-In Yeom 96, 97 Seon-Woo Oh 234, 247, 249, 250 Seong-Bum Baek 114 Seong-Gyu Jang 75, 105 Seong-Im Park 123 Seong-Kon Lee 234, 247, 249, 250 Seong-Ryong Kim 222, 259 Seong-Woo Cho 23, 24, 88, 129, 130, 215 Seong-Wook Kang 23, 24, 215 Seowon Choi 229 Seoyun Yu 216 Seuk Bo Song 126 Seul-Gi Park 11, 51 Seula Choi

354 Name Page Name Page Name Page Seung Hee Choi 113 Seung Hee Han 101 Seung il Yoo 19 Seung Jun Park 191 Seung Tae Kim 239 Seung Uk Ji 111 Seung Woo Jin 179 Seung-A Baek 260 Seung-Ho Paek 259 Seung-Hyun Cho 154 Seung-Hyun Kim 83 Seung-In Yi 89 Seung-Kook Choi 56 Seung-Yeob Song 157 Seunghoon Baek 232 Seunghye Park 174 Seungill Kim 96 Seungmee Jung 187 Seungsu Han 236 Shimon Meir 32 Shin-Chul Bae 104 Shin-Jae Kang 199 Si Hyeock Lee 101 Si Wan Ryu 200 Si-Hwan Ryu 26 Si-Yong Kang 71, 107, 142, 143, 144, 149, 150 Sin-Gi Park 161 Siwon Choi 203 So Hyeon Back 247 So Hyeon Park 192, 193 So Yeon Kang 148 So Yeon Kim 148 So Young Park 243, 245, 251 So-Hee Shin 154 So-Hyeon Baek 228 So-Jin Lee 157 So-Ra Choi 154 So-Yeon Park 75, 105 So-Yoon Seong 239 So-Young Kim 111 So-Young Lee 234, 247, 250 So-Young Park 95 Sok Young Lee 178 Sol-Ji Lee 54 Solji Lee 86 Song Lim Kim 16, 63, 100, 206 Song Samnang 276 Song-Yee Lee 154 Songhwa Chae 43, 144 SongJae Ok 139 Sonia Philosoph-Hadas 32 Soo Im Chunga 217 Soo In Lee 105, 178 Soo Jeong Kwon 129 Soo Jin Wi 189 Soo Min Cho 133 Soo-Cheul Yoo 15, 70, 83, 121, 208 Soo-Hyun Lim 213, 214 Soo-In Sohn 70, 139 Soo-Jeong Kwon 88, 129, 130 Soo-Jung Han 203 Soo-Yun Park 234, 247, 249, 250 Sook Kim 81, 83 Soon Ju Park 174, 209, 267 Soon Ki Park 68, 126, 152, 258 Soon-Chun Jeong 33, 101 Soon-Jae Kwon 141, 144, 150, 166 Soon-JaeK won 149 Soon-Ki Park 258 Soon-Sung Choi 149 Soon-Wook Kwon 75, 83, 105 Soongon Jung 221 Sothea Ouk 92, 145, 146 Sovetgul Asekova 66, 67, 170 Soyoung Park 25, 222 Su Eun Lim 26 Su Hyun Park 232 Su Jang 197 Su Jin Nam 217 Su Ryun Choi 132 Su-Hyang Song 105 Su-Hyun Yun 206 Su-Jeong Kim 137, 138, 187 Su-ji Bae 267 Su-Jin Choi 155, 197 Su-Min Jo 91 Su-Yeon Kim 25, 222 Su-Young Hong 137, 138, 187 Sue Yeon Lee 239 Suhyeon Shim 100, 208 Sujin Lee 89 Sujung Ra 110 Suk Bok Pae 66 Suk-Bok Pae 66, 67, 170 Suk-Ha Lee 69, 89, 120, 130, 207 Suk-Jun Moon 110 Suk-Man Kim 98 Sukgui Oh 209 Sukha Lee 118 Sukman Park 206 Sukyeung Lee 125, 137, 166, 176 Sumin Jo 55, 117 Sun Gang Choi 154 Sun Ha Kim 17, 19, 123 Sun Hyun Chang 189, 230 Sun Mi Huh 194 Sun-geum Jeong 37 Sun-Hee Woo 88, 129, 130, 145 Sun-Hwa Ha 242, 243 Sun-Hyung Lim 18, 44, 131, 155, 158, 183, 197, 207 Sun-Lim Kim 114 Sun-Woo Oh 70 Sun-yi Lee 175 Sun-Young Ko 89 Sung Aeong Oh 68, 126, 258 Sung Cheol Koo 192, 193 Sung Chul Lee 184, 185, 221 Sung Hwan Kim 229 Sung Jin Choi 170 Sung Min Han 71 Sung Un Huh 245 Sung Up Kim 66 Sung-Aeong Oh 258 Sung-Dug Oh 70, 139 Sung-Hwan Jo 145, 170, 271 Sung-Il Lee 71 Sung-Up Kim 66 Sunggil Kim 216 Sunghan Kim 199 Sungtaeg Kang 33, 101, 205 Sungup Kim 67, 170 Sungwoo Lee 9 Sungwook Kim 225 Sungyu Yang 169 Sunha Kim 121 Sunok Moon 242 Sunyoung Yang 186 Susan B. Altenbach 4, 155, 183 Susan McCouch 192 Susan R. Strickler 3 Suyeon Kim 109, 194 Suyun Moon 19,

355 Name Page Name Page Name Page Swapan Kumar Roy 129, 130 Tackmin Kwon 165 Tae Ho Ryu 113 Tae Joung Ha 66, 67 Tae Jung Ha 170 Tae Sun Kang 148 Tae-Heon Kim 55, 91, 117 Tae-Ho Ham 114 Tae-Ho Kim 28, 71, 145, 161 Tae-Ho Lee 173 Tae-Houn Kim 236 Tae-Hwan Jun 33, 151 Tae-Jin Yang 20, 124, 177, 179, 199 Tae-Seok Kwak 103 Tae-Sung Park 134 Tae-Young Heo 57 Taegyu Kim 114 Taejun Kim 93 Taek-Gyu Kang 23, 24, 215 Taeklim Lee 101 Taewook Kim 25, 185 Taeyoung Lee 207 Taeyoung Um 230 Takayuki Kohchi 261 Tao Zhang 189 Tengfang Ling 259 Thanh Dat Mai 73, 104 Thanh-Tam Ho 95 Thank Dat Mai 74 Thi Hoai Thuong Nguyen 258 Thuy Thi Le 128 Toni Mohr 4 Trang Thi Do 70 Ujjal Kumar Nath 276 Un-Sang Yeo 66 Ung-Han Yoon 28, 71, 161 Vikranth Kumar 196 Vimalraj Mani 25, 222 Vo Ngoc Linh Giang 177 Wan Woo Yeam 167, 253 Wan Woo Yeom 119 Wang Xiaohan 45 Wen-Xing Hu 241, 254 Wenzhu Yang 189 Wi-Young Lee 86 Will Urquhart 132 Woe-Yeon Kim 262 Won-Chan Kim 260 Won-Hee Kang 96, 97 Won-Ki Hong 93, 94 Won-Kyung Lee 124 Won-Tae Yang 61 Won-Woo Jo 86 Won-Yong Song 17 Wonwoo Cho 54 Woo Chul Ju 131 Woo In Jung 131 Woo Jin Kim 239 Woo Joo Jung 211 Woo Sik Chung 106 Woo-Jin Kim 19, 121 Woo-Jong Hong 189 Woojong Jang 177 Wook Jin Kim 169 Woon Ji Kim 33, 107, 142, 143 Woon-Cheol Shin 51 Woonhee Baek 185 Xiao-Xuan Du 225 Xiaona Yu 132 Xuefei Yang 120 Y-G Kim 281 Y. Yasui 88 Yang Jae Kang 60 Yang Qin 134 Ye Rim Lee 158, 202, 245, 251 Ye Sol Jeong 242, 243 Ye-Ji Lee 28, 161 Ye-Jin Jang 134 Yedomon Ange Bovys Zoclanclounon 71 Yeeun Jang 177, 179 Yeo Jin Jung 158, 202, 251 Yeo Jin Lee 242 Yeo Jin Youn 216 Yeon Ho Je 239 Yeon-Gyeong Park 223 Yeon-Hee Lee 27, 127 Yeon-Ho Park 213, 214 Yeon-Ki Kim 43, 144 Yeong Deuk Jo 71, 166 Yeong-Ho Kwon 55, 91 Yeongmok Lee 236 Yeonhwa Jo 188 Yi Lee 159, 192, 193, 206 Yinbo Ma 132 Ying Fang 239 Yinping Jiao 232 Yo-Han Yoo 16 Yong Bog Kim 154 Yong Eui Choi 247, 261 Yong Pyo Lim 132 Yong Q. Gu 4 Yong Sic Hwang 194 Yong Weon Seo 87, 103, 122, 166, 210, 211, 213 Yong-Chul Kim 66 Yong-Eui Choi 28, 228 Yong-Gu Cho 14, 88, 92, 145, 146, 192 Yong-Hee Kwon 157 Yong-Jae Won 206 Yong-Jin Park 78, 79, 80, 81, 82, 83, 85, 122, 127, 205 Yong-Jin Shin 203 Yong-Min Kim 96 Yong-Sam Jeon

356 Name Page Name Page Name Page Yong-Sung Park 257 Yong-Wook Ban 35 Yoo-Hyun Cho 81, 83, 205 Yoo-Jung Yoon 209 Yoojin Kim 164 Yoon Hee Jang 135, 224 Yoon Kyung Lee 93 Yoon-Jung Hwang 131 Yoon-Mok Pahk 43 Yoon-Sun Hur 199 Yoon-Sup So 57, 58 Yoonha Kim 10, 223 Yoonjung Lee 114 Yoram Choi 256 You Chun Song 133 You-Chun Song 55, 84, 91, 117 You-Ran Jang 155, 183, 197 Youjin Lee 214 Youn Jung Choi 160 Youn Shic Kim 239 Youn Sung Kim 153 Youn-Soo Choi 23 Youn-Sung Kim 37 Young Chul Park 206 Young Eun Jang 150 Young Hee Joung 73, 74, 104 Young hee Joung 72 Young Ho Kwon 133 Young Jeon 191 Young Jin Park 260 Young Koung Lee 232 Young Ran Lee 160 Young Sam Go 113, 114 Young-Chan Cho 11, 51, 84 Young-Chul Jeon 81, 83 Young-Eun Song 154 Young-Hoon Park 32 Young-Im Choi 18 Young-Jin Kim 36 Young-Joo Jung 242, 243 Young-Joo Seol 110, 246 Young-Ju Oh 70 Young-Saeng Kim 123 Young-Sang Lee 80, 83, 205 Young-Soo Chung 119, 167, 234, 247, 253 Young-Sun Riu 261 Young-yi Lee 124 Youngho Koh 148 Younghoon Park 90 Youra Hwang 76 Yu Jin Jung 158, 202, 245, 251 Yu Jin Kim 172 Yu Mi Choi 166 Yu-Ji Lee 122 Yu-Jin Jung 92, 192 Yu-Mi Choi 125, 137, 176 Yu-Na Kang 133 Yuan Cao 78, 83 Yul-Ho Kim 137, 138, 187 Yun Gyeong Lee 19, 133 Yun Im Kang 160 Yun Jeong Kim 106 Yun Su Do 99 Yun Young Kim 234 Yun-A Jeon 17, 19, 121, 123 Yun-Joo Kang 145 Yun-Shil Gho 16, 117, 242 Yuna Kang 195 Yunde Zhao 189 Yunfei Wu 189 Zachary Lippman 267 Zhanguo Xin 232 Zhi-Fang Zuo 118 ZhongZe Piao 9 Zhuo Jin 36 朴钟泽 241 강경호 43, 52, 97 강범규 41, 75, 102, 175 강석범 44 강성택 85, 102 강세라 237 강승미 179 강용구 40, 77 강유나 200 강인정 95 강주원 99, 176, 215 강준원 86 강천식 37, 38, 39, 40, 41, 64, 150 강철홍 163 강형식 29 강홍규 248, 250 고산 77 고세리 143, 190, 231 고영삼 34, 116, 147 고재권 43, 52, 97, 115 고종민 17, 75, 84, 156, 162, 176, 215 고종철 52, 76 고지연 46, 76 고홍민 102 공도훈 42 곽도연 41, 46, 75, 76, 175 곽영신 231 곽지은 31 구다은 30 권석주 77 권순욱 72 권영호 99, 176, 215 권오덕 29 권용락 30, 64 권진혁 42 권혁주 57 김경민 84, 156, 162 김경혜 102 김경호 37, 38, 39, 40, 41, 150 김경화 107 김경환 59, 61, 62, 63, 223, 244 김경훈 84, 156, 162 김기영 43, 52, 97 김남신 272 김년희 59, 61, 62, 63, 244 김대현 49 김도훈 252 김동용 72 김둘이 107 김문종 50,

357 Name Page Name Page Name Page 김미정 147 김민수 85 김민식 246 김민주 74 김병주 162 김보경 11, 43, 51, 52, 53, 97, 115 김상규 49 김상수 53, 163 김상열 29 김석만 11 김선림 34, 116, 147 김선하 212 김성민 102 김성용 29, 40, 54 김성훈 78, 79 김송림 59, 61, 62, 63, 244 김수 98 김수민 222 김양길 37, 38, 39, 40, 41, 64, 150 김영군 237 김영진 84, 156, 162 김영천 157, 200, 201 김옥례 49, 91 김용호 146 김우재 43, 52, 97 김우진 212 김유진 239 김율호 95 김은경 42 김재광 238 김정인 76 김정주 43, 52, 97, 115 김정태 34, 116, 147, 162 김종미 226 김종민 252 김준식 257 김지민 102 김지영 231 김진주 251 김창기 235 김춘송 43, 51, 52, 53, 73, 97 김태균 29 김태헌 176, 215 김판기 86 김현태 41, 75, 175 김형석 88, 257 김혜림 49 김혜진 235 김호방 64 김홍식 41, 75, 146, 175 김효용 239 남경남 50, 164 남궁민 42, 50, 147, 164, 277 남민희 29, 31 남상식 77 남정권 43, 51, 52, 53, 73, 97, 115 노현수 246 도재왕 217 류민하 64 류시환 42, 50, 147, 164, 277 류호진 58 마경호 46, 78, 79 마무현 146 문선옥 239 문성윤 237 문영일 44 문중경 59, 62, 76 문지혜 49, 98 박기진 42, 50, 147, 164, 277 박노봉 29, 31 박단비 65, 120 박도이 195 박동금 98 박동수 29, 31, 99, 176, 215 박명렬 48, 95 박병명 31 박부희 61 박석만 74 박성임 251 박소연 72 박수윤 237, 238, 249 박수철 222 박순기 125 박슬기 43, 51, 52, 53, 73, 97 박영두 236, 240 박영은 40, 55, 56, 68 박은준 49 박장환 41, 76, 175 박재호 74 박정호 235, 238 박종숙 49 박종열 42, 50, 147, 164, 277 박종호 37, 38, 39, 40, 41, 64, 150 박지영 235 박지희 224 박태성 49, 98, 262 박태일 37, 38, 39, 40, 41, 64, 150 박향미 27, 31, 49, 107, 112, 135 박현근 42 박현수 43, 51, 52, 53, 73, 97 박형호 64 박희영 4 방준형 58 배도영 239 배성우 259 배은경 65, 120 배정숙 39, 41 배환희 34, 116, 147, 162 백만기 43, 51, 52, 53, 73, 97 백성범 34, 116, 147, 162 백소현 233 백정호 59, 61, 62, 63, 244 백형진 46 사무엘티라훈 195 서민정 48, 75, 95 서상기 277 서상재 237, 249 서석철 233, 248 서은정 223, 224 서정필 27, 31, 52, 73,

358 Name Page Name Page Name Page 서정현 41, 75, 175 선현진 233, 248, 250 성낙식 31, 135 성동렬 275 세바스틴 78 소문수 125 손미연 87 손범영 34, 116, 147, 162 손수인 233, 238 손영진 237 손재한 37, 38, 39, 40, 41, 64, 150 손지영 42 손지희 250 송민경 254 송석보 76 송승운 29 송영은 77 송원종 163 송유천 17, 29, 31, 99, 176, 215 송윤호 53, 54, 163 송재기 39, 41 송종태 85 송현진 64 신공식 252, 262 신동범 112 신동진 84, 156, 162 신명재 78, 79 신상욱 41, 75, 175 신운철 43, 52, 97 신윤희 236 심규찬 212 심동환 179 안광환 42 안상낙 212 안억근 27, 31, 49, 107, 112, 135 안영은 91 안율균 91 안은숙 244 안지영 179 안혜련 61, 239 안혜진 57 양대화 250 양선목 38, 39, 40 양은영 49, 98, 195 양정욱 77 양종목 244 엄민식 235 여운상 29 오명규 31 오선우 249 오성덕 233, 237, 238, 249 오성앵 125 오성환 31 오세관 31, 135 오은영 41 오인여 31 오혜진 53, 163 옥현충 79 왕샤오한 78 용우식 50, 147, 164 우관수 179 우관식 76 우영회 91 우은택 236, 240 우제훈 64 원용재 27, 31, 49, 112, 135 유경단 77 유병태 259 유요한 239 유의수 271 윤건식 39 윤도원 233, 237, 238 윤문섭 46 윤미라 31, 112, 135 윤상대 125 윤석원 50, 147, 164 윤수현 44, 74 윤영미 37, 38, 39, 40, 41, 64, 150 윤재복 217 윤철원 246 윤호성 251 윤홍태 41, 48, 75, 95 은창욱 237 이강섭 240 이강현 217 이건미 43, 51, 52, 53, 73, 97 이경민 249 이경보 77 이경준 46 이관호 91 이금선 49 이기안 78, 79 이다솜 125 이덕렬 77 이동규 97 이동희 248 이미자 41 이범규 231, 255 이병원 41, 75 이상구 249 이상복 27, 112 이상협 157, 200, 201 이샛별 176, 215 이성곤 249 이솔지 86 이송이 77 이수경 37 이연희 223, 224 이영규 40, 56 이영기 95 이영복 43 이영실 87 이영훈 41, 75, 95 이옥진 49 이운석 88, 257 이위영 86 이유영 147 이유정 146 이윤영 87 이은경 59, 61, 62, 244 이인석 77 이점호 11, 27, 37, 38, 39, 40, 41, 43, 52, 64, 97, 115, 135, 150 이정동 85, 262 이정로 78, 79 이정희 27, 31, 49, 112, 135 이제완 179 이종희 29, 31, 99, 176, 215 이주석 102 이주영 143, 190, 201, 231 이주원 146 이주희 146 이준구 91 이준대 195 이지우 53, 54, 163 이지윤 29, 31, 99, 176, 215 이진석 34, 116, 147, 162 이진원 125 이창민 51, 52, 73 이택림

359 Name Page Name Page Name Page 이하얀 87 이향기 254 이현석 86 이현숙 212 이형운 77 이혜리 54 이홍석 61, 62, 63, 244 이효신 65, 120 이효연 233, 248, 250 이효정 53 임명호 252, 262 임수미 222 임주성 40, 55, 56, 68 임혜민 86 장길수 49 장동칠 40, 55, 56, 68 장성율 88 장안철 231, 233, 237, 238 장은하 147 장재기 27, 135 장지선 39 장지영 143, 190, 231 장창순 240 전윤아 212 전재범 107 정건호 147 정국현 27, 31, 49, 112, 135 정기홍 239 정영근 38, 39, 40, 150 정영민 87 정옥철 248 정응기 27, 31, 49 정재환 54 정종민 11, 43, 51, 52, 97, 115 정종욱 57, 58 정지영 102 정지웅 11, 43, 52, 97, 115 정지희 64 정태욱 76, 162 정하나 250 정혜린 248 정황원 224 조광수 40, 55, 56, 68 조규택 78, 79 조다님 85 조만현 40 조명철 195 조민식 146 조병관 30 조수민 99, 176, 215 조승현 77 조영래 53, 163 조영일 87 조영찬 43, 51, 52, 53, 73, 97 조원우 86 조윤상 53, 54, 163 조윤성 252, 262 조준현 17, 29, 31, 99, 176 조지홍 40, 55, 56, 68 조현석 249 좌지방 250 주혜준 30 지현소 59, 61, 62, 87, 215, 244 진민아 107 진성범 44, 74 진일두 248 차아라 157 차진경 84, 156, 162 채수영 49, 91, 98, 195 천충기 40, 68 최규환 64, 143, 190, 231 최다은 201 최도진 146 최만수 41, 59, 62, 107 최명은 46, 76 최성태 42 최승출 42, 50, 164 최아영 271 최영임 65 최용의 233 최유미 76 최인찬 59, 61, 62, 63, 244 최장규 40, 55, 68 최재근 42, 50, 147, 164, 277 최준근 42, 50, 147, 164, 277 최지원 65, 120 최진경 41 최창현 37, 38, 39, 40, 41, 64, 150 최현모 65, 120 페레즈무카사 30 하건수 53, 54, 163 하보근 85 한상익 17 한상훈 102 한선경 77 한옥규 39, 41, 64 한원영 41 한정헌 224 한지훈 200 허온숙 49 허윤찬 98 허진호 102 현웅조 27, 31, 49, 112, 135 현종내 162 홍경낙 179 홍규현 91 홍기흥 64 홍순영 29 홍우종 239 홍준기 223, 224 홍하철 27, 31, 112, 135 황엄지 77 황인덕

360 인쇄 2018년 7월 2일 발행 2018년 7월 11일 발행자 사단법인한국육종학회 경기도수원시권선구수인로 126 국립식량과학원중부작물부내 인쇄처 교보문고 ( ) 경기도파주시광인사길 226 두성종이빌딩 4층