KISEP Experimental Research J Korean Neurosurg Soc , 2001 흰쥐에서체감각유발장전위의기록부위별특성과경로분석 * 신현철 박용구 * 이배환 * 류재욱 * 조춘식 * 정상섭 * = Abstr

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지훈 하
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1 KISEP Experimental Research J Korean Neurosurg Soc , 2001 흰쥐에서체감각유발장전위의기록부위별특성과경로분석 * 신현철 ** 박용구 *** 이배환 *** 류재욱 *** 조춘식 *** 정상섭 *** = Abstract = Characteristics and Pathways of the Somatosensory Evoked Field Potentials in the Rat Hyun Chul Shin, M.D.,** Yong Gou Park, M.D.,*** Bae Hwan Lee, Ph.D.,*** Jae Wook Ryou,*** Chun Zhi Zhao,*** Sang Sup Chung, M.D.*** Department of Neurosurgery,** School of Medicine, Donga University, Pusan, Korea Department of Neurosurgery, Medical Research Center, Brain Research Institute,*** Yonsei University College of Medicine, Seoul, Korea bjective - Methods Results- Conclusion - KEY WORDS J Korean Neurosurg SocVolume 30July,

2 서론 재료및방법 1. 실험 1: 흰쥐에서체감각피질의기록위치별 SSEFP의파형변화 - J Korean Neurosurg SocVolume 30July, 2001

3 1) 실험대상및방법 실험 2: 흰쥐의체감각피질, 시상과박속핵 (Nucleus gracilis) 에서 SSEFP의특성 1) 실험대상및방법 J Korean Neurosurg SocVolume 30July,

4 1. 실험 1 결과 Bregma 1mm 2mm 3mm Lat. 1mm 5mA 4mA 3mA 2mA 1mA 2mm Threshold2.81.1mA 3mm A Stimulus artifact 0.01mV ms Bregma 1mm 2mm 3mm Lat. 1mm mm B 3mm 0.02mV msec Stimulus artifact Fig. 1. ARecordings of SSEFP monitored at each sites of cerebral cortex during stimulation of left sciatic nerve. Stimulus intensities were ranged from 1mA to 7mA. When stimulus intensity was increased upto 5mA, response frequency and amplitude increased, but beyond 5mA, they showed no change. SSEFP detection at 4 to 6mm lateral and posterior to bregma was failed. BRecordings of SSEFP monitored at each sites of cerebral cortex in each experimental rats during 5mA stimulation of left sciatic nerve. The vertical line indicate corresponding SSEFP mean onset latency, with standard deviation in, with time in msec. 834 J Korean Neurosurg SocVolume 30July, 2001

5 mm Lat A Bregma mm Post. 4mm Lat B Bregma mm Post Fig. 2. AAmplitude distribution of P1 wave of SSEFP according to the recording sites. Stimulus intensity was 5mA. The numbers indicate the percentile size of SSEFP compared to maximal amplitude. BSSEFP N1 wave distribution according to the recording sites. Sciatic nerve stimulation with 5mA and the numbers indicate percentile SSEFP. J Korean Neurosurg SocVolume 30July,

6 2. 실험 2 Contralateral Somatosensory Cortex Contralateral Thalamus Ipsilateral Gracile Nucleus 7mA 6mA 5mA A 5V 5V V ms ms ms 1 Contralateral Somatosensory Cortex Contralateral Thalamus Ipsilateral Gracile Nucleus V B V V ms ms ms Fig. 3. ASimultaneously recorded SSEFPs at three different sitescontrolateral cortex, controlateral thalamus, ipsilateral gracile nucleus. The sciatic nerve was stimulated with varing stimulus intensities ranging from 5mA to 7mA. Note the different amplitude scales. BRepresentative stimulus recordings of SSEFPs at three different sites in all experimental rats. Sciatic nerve was stimulated by a single square pulse of 7mA with 0.1msec duration. 836 J Korean Neurosurg SocVolume 30July, 2001

7 N1 N1 N2 P1 N P1 P2 P2 P3 5V ms A 5V ms B N1 N2 P V ms P2 C Fig. 4. Schematic diagram of averaging SSEFP wave at controlateral cortexa, contralateral thalamus VPLB, and ipsilateral gracile nucleusc with 7mA stimulus intensity. Table 1. Mean peak latency and mean peak-to-peak amplitude of SSEFP at controlateral cortex when SSEFP were simultaneously recorded at three sitescontrolateral cortex, controlateral thalamus, ipsilateral gracile nucleus. Sciatic nerve was stimulated by a single square pulse of 7mA with 0.1msec duration Latency Onset P1 N1 P2 N2 P3 Meanmsec S.D Amplitude Base-P1 P1-N1 N1-P2 P2-N2 N2-P3 MeanV S.D S.D.standard deviation 1) 대측체감각피질에서동시에기록한 SSEFP 2) 대측 VPL에서동시에기록한 SSEP Table 2. Mean peak latency and mean peak-to-peak amplitude of SSEFP at controlateral thalamus VPL nucleus when SSEFP were simultaneously recorded at three sitescontrolateral cortex, controlateral thalamus, ipsilateral gracile nucleus. Sciatic nerve was stimulated by a single square pulse of 7mA with 0.1msec duration Latency Onset N1 P1 N2 P2 Meanmsec S.D Amplitude Base-N1 N1-P1 P1-N2 N2-P2 MeanV S.D S.D.standard deviation 3) 동측박속핵에서동시에기록한 SSEP J Korean Neurosurg SocVolume 30July,

8 Table 3. Mean peak latency and mean peak-to-peak amplitude of SSEFP at ipsilateral gracile nucleus when SSEFP were simultaneously recorded at three sitescontrolateral cortex, controlateral thalamus, ipsilateral gracile nucleus. Sciatic nerve was stimulated by a single square pulse of 7mA with 0.1msec duration Latency Onset N1 P1 N2 P2 Meanmsec S.D Amplitude Base-N1 N1-P1 P1-N2 N2-P2 MeanV S.D S.D.standard deviation 4) 동시에기록한체감각피질, VPL, 그리고박속핵의 SSEP 의비교 838 Table 4. Conduction velosities of each sites, onset latency, peak latency of waves and estimated latencymsec according to nucleus gracilis onset latency Cortex OL 1 P1 N1 P2 N2 P3 Latency SD E. Lat CV VPL 5 OL N1 P1 N2 P2 Latency SD E. Lat CV Nuc. gra. 6 OL N1 P1 N2 P2 Latency SD CV onset latencymsec 2standard deviation 3estimated latencymsec according to nucleus Gracilis onset latency OLcortex or VPLOLNuc. GraNucleus Gracilis peak latency 4conduction velositym/sec Distancecortexstimulus=194.58mm, VPLstimulus= mm Nucleus Gracilisstimulus = mm 5ventro posterolateral nucleus of thalamus 6nucleus gracilis J Korean Neurosurg SocVolume 30July, 2001

9 고찰 J Korean Neurosurg SocVolume 30July,

10 결론 840 References 1) Aminoff MJ:Use of somatosensory evoked potentials to evaluate the peripheral nervous system. J Clin Neurophysiol 51: , ) Di S, Brett B, Barth DS:Polysensory evoked potentials in rat parietotemporal cortex:combined auditory and somatosensory responses. Brain Res 642: , ) Eisen A:The somatosensory evoked potential. Can J Neurol Sci 9:65-77, ) Fehlings MG, Tator CH, Lindon RD:The relationships among the severity of spinal cord injury, motor and somatosensory evoked potentials and spinal cord blood flow. Electroenceph- J Korean Neurosurg SocVolume 30July, 2001

11 alogr Clin Neurophysiol 74: , ) Fehlings MG, Tator CH, Lindon RD, Piper IR:Motor evoked potentials recorded from normal and spinal cord-injured rats. Neurosurgery 20: , ) Fehlings MG, Tator CH, Lindon RD, Piper IR:Motor and somatosensory evoked potentials recorded from the rat. Electroenceph Clin Neurophysiol 69:65-78, ) Hiraizumi Y, Transfeidt EE, Kawahara N, Yamada H:Differences in sensitivity between magnetic motor-evoked potentials and Somatosensory-evoked potentials in experimental spinal cord lesions. Spine 21: , ) Hurlbert RJ, Koyanagi I, Tator CH:Sensory evoked potentials for selective monitoring of the rat spinal cord:a cerebellar evoked potential to assess ventral cord integrity. J of Neurotrauma : , ) Kim JH, Park YG, Cheon JS, Levy WJ:Cerebellar evoked potentials and motor evoked potentials in the spinal cord of rats. Electroencephalogr Clin Neurophysiol 1: , 1996 ) Koralek KA, Jensen KF, Killackey HP:Evidence for two complementary patterns of thalamic input to the rat somatosensory cortex. Brain Res 463: , ) Koyanagi I, Tator CH:The effects of cortical stimulation, anesthesia and recording site on somatosensory evoked potentials in the rat. Electroencephalogr Clin Neurophysiol 1: , ) Nashmi R, Imamur H, Tator CH, Fehlings MG:Serial recording of somatosensory and myoelectric motor evoked potentials:role in assessing functional recovery after graded spinal cord injury in the rat. J of Neurotrauma 14: , ) Nothias F, Peschanski M, Besson JM:Somatotopic reciprocal connections between the somatosensory cortex and the thalamic Po nucleus in the rat. Brain Res 447: , ) Park YG, Chung SS, Lee KC, Cho J, Jang JW, Kim JH: Monitoring of Pyramidal Motor Evoked Potentials in the Rat. J Korean Neurosurg Soc 25(5): , ) Paxinos G, Watson C:The rat brain in stereotaxic coordinates 2nd ed, ) Raines A, Dretchen KI, Marx K, Wrathall JR:Spinal cord contusion in the rat:somatosensory evoked potentials as a function of graded injury. J Neurotrauma 5:151-1, ) Sakatani K, Iizuka H, Young W:Somatosensory evoked potentials in rat cerebral cortex before and after middle cerebral artery occlusion. Stroke 21: , ) Skarphedinsson JO, Delle M, Carlsson S, Bealer SL:The effects of hexamethonium on cerebral blood flow and cerebral function during relative cerebral ischaemia in rats. Acta Physiol Scand 158:21-28, ) Synek VM:Role of somatosensory evoked potentials in the diagnosis of peripheral nerve lesions:recent advances. J Clin Neurophysiol 4:55-73, ) Wiederholt WC, Iragui-madoz VJ:Far field somatosensory potentials in the rat. Electroencephalogr Clin Neurophysiol 42: , ) Yiannikas C:Short-latency somatosensory evoked potentials in peripheral nerve lesions, plexopathies, radiculopathies, and spinal cord trauma. In Chiappa KH, ed:evoked Potentials in Clinical Medicine. New York, Raven Press, 1989, pp ) Zhao S, Kim DH, Kline DG, Beuerman RW, Thompson HW: Somatosensory evoked potentials induced by stimulating a variable number of nerve fibers in rat. Muscle & Nerve 16: , ) Zileli M, Schramm J:Motor vs somatosensory evoked potential changes after acute experimental spinal cord injury in rats. Acta Neurochir(Wien) 8: , 1991 J Korean Neurosurg SocVolume 30July,

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