- PDF Free Download

Size: px

Start display at page:

Download ""

정성 미
6 years ago
Views:

1 저작자표시 - 비영리 - 변경금지 2.0 대한민국 이용자는아래의조건을따르는경우에한하여자유롭게 이저작물을복제, 배포, 전송, 전시, 공연및방송할수있습니다. 다음과같은조건을따라야합니다 : 저작자표시. 귀하는원저작자를표시하여야합니다. 비영리. 귀하는이저작물을영리목적으로이용할수없습니다. 변경금지. 귀하는이저작물을개작, 변형또는가공할수없습니다. 귀하는, 이저작물의재이용이나배포의경우, 이저작물에적용된이용허락조건을명확하게나타내어야합니다. 저작권자로부터별도의허가를받으면이러한조건들은적용되지않습니다. 저작권법에따른이용자의권리는위의내용에의하여영향을받지않습니다. 이것은이용허락규약 (Legal Code) 을이해하기쉽게요약한것입니다. Disclaimer

5 - i -

6 - ii -

7 - iii -

8 - 1 -

9 - 2 -

10 ~ ~ ~ ~ - 3 -

11 ~ - 4 -

12 - 5 -

13 ~ ~ ~ - 6 -

14 ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~3 ~ ~ ~ ~ ~ - 7 -

15 - 8 -

16 6~ ~42 8.4~ ~ ~40 19~22.5 5~9 2~9 7~8 7~19 8~13 5~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~9 12~22 9~11 12~21 14~29 19~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~1.4 absent present present present present present - 9 -

17 ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~

18 ~ ~ ~ ~

19 ~ ~ ~ ~ ~ ~ ~ ~ ~

20 - 13 -

21 ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~

22 ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~

23 Fig. 5. Leontopodium leiolepis Nakai A, habit; B, outer involucral scales; C, middle involucral scales; D, inner involucral scales

24 Fig. 6. Holotype specimen of Leontopodium leiolepis Nakai (Waigalbon, 2200m, 11 July 1914, T. Nakai, n. 1542, (TI!))

25 ~ ~ ~ ~ ~ ~ ~ ~

26 - 19 -

27 - 20 -

28 Taxon No. Localities Voucher 1 Gyeonggi-do, Mt. Unak Lee L. japonicum 2 Gangwon-do, Mt. Seolak Lee Gyeongsangbuk-do, Mt. Sobaek Lee L. hallaisanense 8 Jeju-do, Mt. Halla Lee Jeju-do, Mt. Halla Lee Gangwondo, Mt. Seolak Lee L. leiolepis 11 Gangwondo, Mt. Seolak Lee Gangwondo, Mt. Seolak Lee Gyeongsangbuk-do, Mt. Bibong Lee L. leontopodioides 14 Gyeongsangbuk-do, Mt. Dukam Lee Gyeongsangbuk-do, Mt. Sobaek Lee Taxon Localities Accession L. alpinum Italy, Piemonte FJ L. alpinum Spain, Aragon FJ L. andersonii China, Yunnan FJ L. artemisiifolium China, Yunnan FJ L. caespitosum China, Yunnan FJ L. calocephalum China, Sichuan FJ L. dedekensii China, Yunnan FJ L. franchetii China, Yunnan FJ L. haastioides China, Tibet/Xizang FJ L. himalayanum China, Tibet/Xizang FJ L. jacotianum Nepal, Langtang FJ L. kurilense - AF L. leontopodioides Mongolia, Hövgöl Aimak FJ L. microphyllum Taiwan, Hualien FJ L. nanum China, Xizang FJ L. nivale Italy, Abruzzo FJ L. pusillum China, Tibet/Xizang FJ L. sinense China, Yunnan FJ L. souliei China, Yunnan FJ Anaphalis javanica - U95292 Antennaria carpatica Austria, Salzburg FJ A.dioica Austria, Niederösterreich FJ Ewartia meredithiae - AF Gnaphalium hoppeanum Italy, Lombardia FJ

29 Primer Sequence Length Reference ITS1 5'-TCCGTAGGTGAACCTGCGG-3' 19 mer White et al. (1990) ITS2 5'-GCTGCGTTCTTCATCGATGC-3' 20 mer White et al. (1990) ITS3 5'-GCATCGATGAAGAACGCAGC-3' 20 mer White et al. (1990) ITS4 5'-TCCTCCGCTTATTGATATGC-3' 20 mer White et al. (1990) ITS5 5'-GGAAGTAAAAGTCGTAACAAGG-3' 22 mer White et al. (1990) Primer Sequence Length Reference rpl16 5'-GTTTCTTCTCATCCAGCTCC-3' 20 mer Nishizawa and Watano (2000) rpl16 5'-GAAAGAGTCAATATTCGCCC-3' 20 mer Nishizawa and Watano (2000) rps16 F 5'-CCCCCTAGAAACGTATAGGA-3' 20 mer Nishizawa and Watano (2000) rps16 R 5'-CGAAGTAATGTCTAAACCCA-3' 20 mer Nishizawa and Watano (2000) petd-rpoa F 5'-GGGCATTGGTGCAACATTAC-3' 20 mer Nishizawa and Watano (2000) petd-rpoa R 5'-CAGCCAAGAAGATCTTATGA-3' 20 mer Nishizawa and Watano (2000) trnl5' UAA F(TabC) 5'-CGAAATCGGTAGACGCTACG-3' 20 mer Taberlet et al. (1991) trnf5' GAA (TabF) 5'-ATTTGAACTGGTGACACGAG-3' 20 mer Taberlet et al. (1991) trnh-psba 5'-CGCGCATGGTGGATTCACAATCC-3' 23 mer Sang et al. (1997) psba 5'-GTTATGCATGAACGTAATGCTC-3' 22 mer Sang et al. (1997) atpf 5'-ACTCGCACACACTCCCTTTCC-3' 21 mer Shinozaki et al. (1986) atph 5'-GCTTTTATGGAAGCTTTAACAAT-3' 23 mer Deno et al. (1984)

30 ~ ~ ~ ~ Table 7. Sequence divergence among Korean Leontopodium species based on the Kimura 2-parameter method L. japonicum - 2. L. japonicum L. japonicum L. hallaisanense L. hallaisanense L. leontopodioides L. leontopodioides L. leontopodioides L. leiolepis L. leiolepis L. leiolepis

31 ~ ~ ~ ~ Table 8. Size and G+C contents of ITS1, 5.8S and ITS2 regions of nrdna from four species of Korean Leontopodium. ITS1 5.8S ITS2 Total Length range(bp) Number of indels Substitution G+C content(%) 44.5~ ~ ~49.4 Table 9. Size and G+C contents of 7 non coding regions of cpdna from four species of Korean Leontopodium. atpf-h rps16_1 rps16_2 petd-rpoa trnh-psba rpl16 trnl-f Length range(bp) Number of indels Substitution(bp) G+C content(%)

32 - 25 -

33 Fig. 7. The 50% majority consensus tree of maximum parsimony tree of Korean Leontopodium based on ITS sequence data. Numbers above the branches indicate bootstrap percentages from 1000 replications

34 Fig. 8. The 50% majority consensus tree of maximum parsimony tree of Leontopodium based on ITS sequence data. Numbers above the branches indicate bootstrap percentages from 1000 replications( : Korean Leontopodium investigated in this study)

35 - 28 -

36 Table 10. Materials and collection sites of the Korean Leontopodium for studying on chromosome. Taxa Collection sites Vouchers Sect. Nobilia L. hallaisanense Jeju-do, Mt. Halla Lee L. japonicum Gyeongsangbuk-do, Mt. Sobaek Lee Gangwon-do, Mt. Seolak Lee Gyeonggi-do, Mt. Unak Lee Sect. Leontopodium L. leiolepis Gangwon-do, Mt. Seolak Lee L. leontopodioides Gyeongsangbuk-do, Mt. Bibong Lee Gyeongsangnam-do, Mt. Hwangmae Lee

37 ~

Taxa Previous study(2n) Present study(2n) Sect.

japonicum 21 Sakai, 1934; Arano, 1956 28 26 Arano,

9. Somatic chromosomes of four Korean species of

38 Table 11. Chromosome numbers(2n) of Korean Leontopodium species. Taxa Previous study(2n) Present study(2n) Sect. Nobilia Leontopodium coreanum? 24 Lee and Kim, L. hallaisanense 28 Lee and Kim, L. japonicum 21 Sakai, 1934; Arano, Arano, 1963; Lee, 1969 Sect. Leontopodium L. leiolepis L. leontopodioides 26 Marhold, A B C D E F Fig. 9. Somatic chromosomes of four Korean species of Leontopodium. A. L. japonicum (2n=28, Mt. Sobaek); B. L. japonicum (2n=28, Mt. Unak); C. L. japonicum (2n=28, Mt. Seolak); D. L. hallaisanense (2n=28); E. L. leiolepis (2n=24); F. L.leontopodioides (2n=24). Bar=5μm

39 - 32 -

40 - 33 -

41 - 34 -

42 - 35 -

43 - 36 -

44 - 37 -

저작자표시 - 비영리 - 변경금지 2.0 대한민국 이용자는아래의조건을따르는경우에한하여자유롭게 이저작물을복제, 배포, 전송, 전시, 공연및방송할수있습니다. 다음과같은조건을따라야합니다 : 저작자표시. 귀하는원저작자를표시하여야합니다. 비영리. 귀하는이저작물을영리목적으로이용할

저작자표시 - 비영리 - 변경금지 2.0 대한민국 이용자는아래의조건을따르는경우에한하여자유롭게 이저작물을복제, 배포, 전송, 전시, 공연및방송할수있습니다. 다음과같은조건을따라야합니다 : 저작자표시. 귀하는원저작자를표시하여야합니다. 비영리. 귀하는이저작물을영리목적으로이용할수없습니다. 변경금지. 귀하는이저작물을개작, 변형또는가공할수없습니다. 귀하는, 이저작물의재이용이나배포의경우,