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1 Effect of the bovine collagen matrix on bone formation in rat calvarial and mandibular defects Jeong-Ho Yun 1, Jee-Hee Jung 2, Jin-Woo Kim 2, Chang-Sung Kim 2, Seong-Ho Choi 2, Kyoo-Sung Cho 2 1 Department of Dentistry, College of Medicine, Kwandong University, Myongji Hospital 2 Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei University Abstract Bovine collagen matrix used in this study consists of a porous collagen matrix of bovine origin(spongiosa) and has been evaluated as potential candidates for bone regenerative therapy. It has been used in the oral cavity under a variety of indications. This study was performed to evaluate the bone formation effect of the bovine collagen matrix in rat calvarial and mandibular defects. Critical-sized eight-mm calvarial defects and five-mm mandibular defects were created in 60 male Sprague-Dawley rats using trephine bur. The animals were divided into 4 groups. Each group received one of the following: Control(Sham-surgery), and experimental(bovine collagen matrix) for calvarial defect and mandibular defects. In the mandibular defect model, both sides of the mandible were used for the experiment. Defects were evaluated by histologic and histometric parameters following 2- and 8-week healing intervals (10 animal/group/healing interval). Overall results were uneventful without any defect exposure or inflammation. The amount of new bone formation and bone maturity increased with the increase in healing period at each group. On histologic observation, a large amount of newly formed bone was observed in the experimental group. Moreover, bovine collagen matrix was observed at 2 weeks, but by 8 weeks, the bovine collagen matrix appeared to be completely absorbed. Histometric analysis revealed that the amount of new bone was significantly greater in the collagen matrix treated sites than in the control at 8 weeks in calvarial defects (P<0.05). Within the limits of the study, these results suggest that the use of the bovine collagen matrix on the calvarial and mandibular defects in rats has a beneficial effect on the regeneration of bone tissue. Key words: bone formation, calvarial defect, collagen matrix, mandibular defect, rat 32 Implantology Vol. 14, No

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Implantology 2010 Jeong-Ho Yun et al: Effect of the bovine collagen matrix on

3 original article Jeong-Ho Yun et al: Effect of the bovine collagen matrix on bone formation in rat calvarial and mandibular defects. Implantology 2010 Jeong-Ho Yun et al: Effect of the bovine collagen matrix on bone formation in rat calvarial and mandibular defects. Implantology Implantology Vol. 14, No

4 Jeong-Ho Yun et al: Effect of the bovine collagen matrix on bone formation in rat calvarial and mandibular defects. Implantology

5 original article Jeong-Ho Yun et al: Effect of the bovine collagen matrix on bone formation in rat calvarial and mandibular defects. Implantology Implantology Vol. 14, No

6 Jeong-Ho Yun et al: Effect of the bovine collagen matrix on bone formation in rat calvarial and mandibular defects. Implantology 2010 Jeong-Ho Yun et al: Effect of the bovine collagen matrix on bone formation in rat calvarial and mandibular defects. Implantology

7 original article Jeong-Ho Yun et al: Effect of the bovine collagen matrix on bone formation in rat calvarial and mandibular defects. Implantology Implantology Vol. 14, No

A B Fig. 8. Representative photomicrograph of calvarial defects receiving the bovine collagen matrix at 8 weeks (B; boxed area in A).

The collagen matrix biomaterial appears completely absorbed ( =defect margin; NB=new bone; OS=osteon; H&E stain; original magnification: A 16, B 100).

Representative photomicrographs of mandibular defects receiving the bovine collagen matrix at 8 weeks (B; boxed area in A).

stain; original magnification: A 20 and B 100).

8 A B Fig. 8. Representative photomicrograph of calvarial defects receiving the bovine collagen matrix at 8 weeks (B; boxed area in A). New bone formation was observed between and besides the margins of the defect. There was observed a lot of consolidations of lamellar bone. The collagen matrix biomaterial appears completely absorbed ( =defect margin; NB=new bone; OS=osteon; H&E stain; original magnification: A 16, B 100). Jeong-Ho Yun et al: Effect of the bovine collagen matrix on bone formation in rat calvarial and mandibular defects. Implantology 2010 A B Fig. 9. Representative photomicrographs of mandibular defects receiving the bovine collagen matrix at 8 weeks (B; boxed area in A). The histological observation of the defect was similar to sham-surgery control ( =defect margin; NB=new bone; CT=connective tissue; MF=muscle fiber; H&E stain; original magnification: A 20 and B 100). Jeong-Ho Yun et al: Effect of the bovine collagen matrix on bone formation in rat calvarial and mandibular defects. Implantology 2010 실험군에서는 2.074±1.074, 5.186±2.190 으로 나타 냈으며, 대조군과 실험군을 비교하였을 때는 2주, 8주 모두에 났다. 2주와 8주를 비교하였을때 대조군과 실험군 모두에서 서 통계학적으로 유의성이 관찰되지 않았다. 통계학적으로 유의하게 8주에서 더 높은 신생골 형성을 나타 대한구강악안면임프란트학회지 14권 1호,

9 original article Jeong-Ho Yun et al: Effect of the bovine collagen matrix on bone formation in rat calvarial and mandibular defects. Implantology 2010 Jeong-Ho Yun et al: Effect of the bovine collagen matrix on bone formation in rat calvarial and mandibular defects. Implantology Implantology Vol. 14, No

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11 original article 1. Patino MG, Neiders ME, Andreana S, et al. Collagen as an implantable material in medicine and dentistry. J Oral Implantol. 2002;28(5): Ueda M, Okubo H, Fujimoto Y, et al. Effect of the atelocollagen sponge on healing of bone defects. Journal of the Japanese Stomatological Society. 1994;43: Liu LS, Thompson AY, Heidaran MA, et al. An osteoconductive collagen/hyaluronate matrix for bone regeneration. Biomaterials. 1999;20(12): Saadeh PB, Khosla RK, Mehrara BJ, et al. Repair of a critical size defect in the rat mandible using allogenic type I collagen. J Craniofac Surg. 2001;12(6): Rocha LB, Brochi MA, Bellucci AD, Rossi MA. Efficacy of polyanionic collagen matrices for bone defect healing. J Biomed Mater Res B Appl Biomater. 2004;71(2): Fuerst G, Reinhard G, Tangl S, et al. Effect of platelet-released growth factors and collagen type I on osseous regeneration of mandibular defects. A pilot study in minipigs. J Clin Periodontol. 2004;31(9): Zambonin G, Grano M. Biomaterials in orthopaedic surgery: effects of different hydroxyapatites and demineralized bone matrix on proliferation rate and bone matrix synthesis by human osteoblasts. Biomaterials. 1995;16(5): Choi BH, Lee SH, Huh JY, Han SG. Use of the sandwich osteotomy plus an interpositional allograft for vertical augmentation of the alveolar ridge. J Craniomaxillofac Surg. 2004;32(1): Pradel W, Eckelt U, Lauer G. Bone regeneration after enucleation of mandibular cysts: comparing autogenous grafts from tissue-engineered bone and iliac bone. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;101(3): Implantology Vol. 14, No

12 10. Takagi K, Urist MR. The reaction of the dura to Bone Morphogenetic Protein(BMP) in repair of skull defects. Ann Surg. 1982;196: Dahlin C, Linde A, Gottlow J, Nyman S. Healing of bone defects by guided tissue regeneration. Plast Reconstr Surg. 1988;81(5): Schallhorn RG, Hiatt WH, Boyce W. Iliac transplants in periodontal therapy. J Periodontol. 1970;41(10): Patino MG, Neiders ME, Andreana S, et al. Collagen as an implantable material in medicine and dentistry. J Oral Implantol. 2002;28(5): Toung JS, Ogle RC, Morgan RF, Lindsey WH. Repair of a rodent nasal critical-size osseous defect with osteoblast augmented collagen gel. Laryngoscope. 1999;109(10): Schlegel KA, Donath K, Rupprecht S, et al. De novo bone formation using bovine collagen and platelet-rich plasma. Biomaterials. 2004;25(23): Zambonin G, Grano M. Biomaterials in orthopaedic surgery: effects of different hydroxyapatites and demineralized bone matrix on proliferation rate and bone matrix synthesis by human osteoblasts. Biomaterials. 1995;16(5): Hubbell JA. Biomaterials in tissue engineering. Biotechnology (N Y). 1995;13(6): Masi L, Franchi A, Santucci M, et al. Adhesion, growth, and matrix production by osteoblasts on collagen substrata. Calcif Tissue Int. 1992;51(3): Hemprich A, Lehmann R, Khoury F, et al. [Filling cysts with type 1 bone collagen]. Dtsch Zahnarztl Z. 1989;44(8): Nimni ME, Cheung D, Strates B, et al. Chemically modified collagen: a natural biomaterial for tissue replacement. J Biomed Mater Res. 1987;21(6): Schmitz JP, Hollinger JO. The critical size defect as an experimental model for craniomandibulofacial nonunions. Clin Orthop. 1986;205: Selvig KA. Discussion : Animal models in reconstructive therapy. J Periodontol. 1994;65: Bosch C, Melsen B, Vargervik K. Importance of the critical-sized bone defect in testing bone-regenerating materials. J Craniofac Surg. 1998;9(4):

00)14-1목차1~3

00)14-1목차1~3 The effect of MBCP TM bone regeneration in osteoporosis induced rats Won Hee Kim, Jeong Kyung Kang, Jae Jun Ryu Dept.of esthetic restorative dentistry, Graduate School of Clinical Dentistry, Korea University