제출문 - 2 -
요약문 - 3 -
- 4 -
- 5 -
- 6 -
- 7 -
- 8 -
- 9 -
- 10 -
- 11 -
- 12 -
- 13 -
- 14 -
- 15 -
- 16 -
- 17 -
- 18 -
- 19 -
- 20 -
- 21 -
- 22 -
- 23 -
- 24 -
- 25 -
- 26 -
- 27 -
- 28 -
- 29 -
- 30 -
- 31 -
- 32 -
- 33 -
- 34 -
- 35 -
- 36 -
- 37 -
- 38 -
- 39 -
3.5 3 2.5 2 1.5 1 CO NT P1 0.5 0 PC NC 1 2 3 4 5 6 7 8 9 10 11 12 13-40 -
- 41 -
- 42 -
- 43 -
- 44 -
- 45 -
- 46 -
- 47 -
- 48 -
- 49 -
- 50 -
품종군 내냉성내냉성내냉성시험 + 흰가루병저항성계통수 + 흰가루병저항성계통수계통수 + 노균병저항성계통수 (%) (%) (%) 백다다기 350 28 (8.0%) 6 (1.7%) 4 (1.1%) 가시 140 31 (22.1%) 23 (16.4%) 11 (7.9%) 취청 110 5 (4.5%) 2 (1.8%) 0 (0%) 합계 600 64 (10.7%) 31 (5.2%) 15 (2.5%) - 51 -
- 52 -
- 53 -
- 54 -
- 55 -
- 56 -
- 57 -
- 58 -
- 59 -
- 60 -
- 61 -
- 62 -
- 63 -
- 64 -
- 65 -
- 66 -
- 67 -
- 68 -
- 69 -
- 70 -
- 71 -
- 72 -
- 73 -
- 74 -
- 75 -
- 76 -
- 77 -
- 78 -
- 79 -
- 80 -
- 81 -
- 82 -
- 83 -
연관그룹번호 1 2 3 4 다형성을보이는 SSR 마커마커수 SSR15755 SSR05124 SSR23487 SSR07133 SSR14445 SSR10134 SSR07405 SSR15581 SSR11340 SSR10550 SSR04928 SSR12258 SSR03860 SSR01215 SSR01091 SSR01816 SSR32614 SSR14619 24 SSR15510 SSR19631 CSWTA05 SSR22501 SSR14697 SSR22403 SSR05492 SSR22653 SSR03610 SSR00289 SSR15172 SSR10522 SSR05420 SSR23420 SSR03606 SSR11908 SSR10064 SSR10518 SSR16621 SSR17108 SSR06678 SSR20045 SSR18362 SSR15172 21 SSR16462 SSR00204 SSR06722 SSR16837 SSR10370 SSR29389 SSR18541 SSR05012 SSR23177 SSR00299 SSR23440 SSR05757 SSR05700 SSR19430 SSR06011 SSR13949 SSR13466 SSR18640 SSR14446 SSR06650 SSR17583 30 SSR23785 SSR03621 SSR21454 SSR04908 SSR03056 SSR19430 SSR04857 SSR14841 SSR14725 SSR23725 SSR12032 SSR07706 SSR07130 SSR02803 SSR16315 SSR04251 SSR14026 SSR02697 SSR04454 SSR16381 SSR19034 SSR11074 SSR03481 SSR29712 SSR07209 SSR06225 SSR07543 SSR17406 SSR04482 SSR20307 18 5 SSR10348 SSR14611 SSR04245 SSR20465 SSR16032 SSR15564 SSR12291 SSR04816 SSR10720 SSR07369 SSR11264 CSWTA04 SSR06447 SSR00398 SSR32717 SSR00170 SSR04323 SSR18259 SSR03943 SSR14193 SSR00648 SSR13639 22 6 7 SSR00259 SSR04751 SSR18718 SSR00300 SSR15955 SSR02086 SSR19803 SSR05946 SSR27117 SSR23856 SSR00126 SSR21936 SSR03940 SSR20074 SSR23109 SSR17818 SSR21886 SSR01179 SSR23037 SSR15606 SSR12833 CSWCT16B C31 SSR18780 SSR00015 SSR00205 SSR19835 SSR31005 SSR20122 SSR27320 SSR14606 SSR14224 SSR22777 SSR16591 SSR00215 SSR23250 SSR10066 SSR14981 SSR29516 24 15-84 -
- 85 -
- 86 -
- 87 -
No. Forward (5 3) Tm Reverse (5 3) Tm M1 catttgaaagttcaacccttctaaaca 53 cctaaactttgaaaagtaacaatttactc 53 M2 aatcctgaagttcatgttgatgtgg 54 ctctctaggccataaactatcctc 55 M3 tgttccactctacctatgacattttga 55 accaaatgtatatgtttttctttgcattt 51 M4 ttgtttacaccgtgtcagtaacttaag 55 cgtgtgtaaaaattaaattcaaaacatttc 52 M5 ttttcgagcaatcaaatggtttttattta 51 tgctactttcttgagatgaaactttttg 54 M6 tgattgtgactttgcaaggaaatg 52 tcttaagagatggcccaaccaa 53 M7 ttgctgaatgcaccaatgga 49 ccatggaaggtattgagaaattgatg 54 M8 tcttaattaaccgagagacacttcc 54 tcgttaaatttgtatacgaaaaatttgat 50 M9 aatctttacttgtatcaatcttctaaattc 52 caatttaaacctcaaaacttctttaaag 51 M10 tgaataaatattttaaggtcgtatacag 51 tccaccaaaccacactactctt 53 M11 cgtctgtgctatacgccaag 53 ttcaagcacttaaagataagccaa 50 M12 tatagaagattagaagaaatacgttg 50 aaccttactagggtttacattcgttt 53 M13 aaacgtgaagtaatgagacctagcgta 56 catttgtaaacatcaattcgccaaa 51 M14 tctctgtgttggtataacgtacca 54 caagctaagtgagaatgtaagtg 51 M15 tacagaatttgtgtttaaattatttgtt 49 atgtgatcaaatacatttataaagg 48 M16 cttatatattgttgatcaagctattg 50 gcacttacgaggataaacaaaatac 53 M17 aggtgcatttgggtagaatgttg 53 cagtgatttatttttgcacggaact 52 M18 tccaaaaagatcaattaactcaagtctca 54 ccggtccatcaactactactcaagg 59 M19 gccccaactcgctcgatg 54 tgaaataagatcacaaaaaggggc 52 M20 gcatacattgaaatgcaacaactca 52 tcccctttagatgtccaataaatgc 54 M21 ttgatgaagaagcgtttacagtatgg 54 agtggatttgaaacgcaaggat 51 M22 tggccttacaattgctattttcca 52 gccttttcctattcgacttttgtct 54 M23 aaagttaactcatcgcaaaattgttg 51 ggccaaaagtattagtccgtag 53 M24 tcaaagtattcattaagaaaattgcat 49 tgaaagggggttagtttttaaaga 50 M25 gttggttaaatatatttttttacaacttg 50 aaacagacaacttttctaatatacg 50 M26 tggcttcttgccagattccatt 53 tctcttgaaagttcctttgagatct 53 M27 aagaactgactataaagattaatttta 48 taactcttagacatgtgagtatac 50 M28 ctaatatggaatgaaaaacaaggttg 52 ttaactcttagacatgtgagtatact 53 M29 caacatcaacatgaccccataa 51 ttgcactggaagttctgaaaagg 53 M30 ctgagaaaaatggcggcaag 51 gggacattgtcgatgagattga 53 M31 gcgattttaatcaaaaatttcagtg 50 tcttccaatatactctctccct 51 M32 tgcaatgtgtcattccccattta 51 ccctatacctctacattcatctc 53 M33 tgtaatttgccattttaagaaccttga 52 gaaggaaaattggttggaatttcg 52 M34 catgtttttaataattaaaaataaatcat 46 atgataaataataacttttagttcg 46 M35 tgatcgtagttattctcccttcaa 52 gaaaaaggactaaaaatgtgaataaataag 52 M36 catcgctttcaaaagaggttgg 53 gaacctccaaatgggattcagg 54 M37 tgcaatcacagaaatggaaccag 53 tcaacttttgatgcaaactgatgg 52 M38 cgcgaagaagaagaggcaga 53 ccctagctttgtgcgacgtt 53 M39 cagtcgctgggccttctttc 55 ttcaggagacaaagcgagacctt 55 M40 tattggatatccacattgtgacag 52 ggatttctgttttgatggatagaac 53 M41 gtaattaaataccacataatatcacaa 49 tgttcaagaggctatacatccta 52 M42 ggattccaaagaatttgttctcca 52 gccttatgggtttaaattcaatactc 53 M43 atagatatgtacgtcgatttctcca 52 taggctttatattttggattaagttg 50 M44 ctttcaagaactattaaccattgc 50 cgcaaggttgtacattgccaaa 53 M45 ttcatattgacacaacttaaaccttg 52 gaggcttacacctctcatagaag 55-88 -
No. Forward Tm Reverse Tm M46 catgcatggagatcatatatattttg 52 acaaaatggagctttgttttgagaa 51 M47 tgctaaggaaggtgggtgacaa 54 tcatgggagaatgtgggtaacaa 53 M48 tttctggtgggagagttgtgttg 55 tctctcatcccatttcaacctca 53 M49 tgggaatagaaaaactccgaacaa 52 ttgcctataaggacggagtttcaa 54 M50 gcatttcttctgttatttttatttttcat 50 gctttgtgctcactgcttatg 55 M51 aactgagatgatgatgtgacaatcaaa 53 acaacaagttcaccatctttagtcgat 55 M52 caacattatattcatttcttcaaagtta 50 ggtaagtgatgaaggtataggtg 53 M53 cataagattcttttgcttcaccattc 53 ctccaaatgatattatgattcacatg 51 M54 tgaagaaatcggcgatggag 51 cccacactgcggatccaac 55 M55 gtattttccaattcaaatcatattcttac 52 acatattacgtagaaatgtgattgga 52 M56 tcccagaagtcaatatcctagtgct 56 ccctgtgtttctgtttgaggaa 53 M57 tccaaatagcttgtatgaaactctgtca 55 tccattcaggaccaatcttgtca 53 M58 gcatgataataatctcaactctgtta 52 tgcaataggctgaagaaattcg 51 M59 gcctctttgcgttgggtcat 53 tcctaaaacctatcatggatctga 52 M60 gcacgaaggaagacaaaagtcg 54 ccagtcgccaccatctaagc 55-89 -
No. Name Source Origin 1 은성백다다기 Heungnong seed 2 새론반백 Danong, Inc. 3 아시아청장 Asia seeds 4 새론청장 Danong, Inc. 5 흑농백다다기 Heungnong seed 6 글로리삼청 Asia seeds 7 백록다다기 Sinjenta seeds, Inc. 8 NC76 - USA 9 조은백다다기 Heungnong seed 10 한강맛백다다기 Sinjenta seeds, Inc. 11 신정품 Dongbu hightech 12 White Dongbu hightech 13 Donggwan Tianjin Germany Switzerland, Inc China 14 GY14 - USA 15 낙동청장 Dongbu hightech 16 장형낙합 Nongwoo bio - 90 -
- 91 -
No. SNP types SNP types HRM분석 No. (GY14 vs. Long4) (GY14 vs. Long4) HRM분석 1 1개,SNP H 31 Indel, SNP M 2 1개,SNP L 32 Indel, SNP L 3 2개 Indel H 33 2개,SNP M 4 indel, SNP L 34 Indel, SNP H 5 indel, SNP L 35 2개,SNP H 6 2개,SNP H 36 Indel, SNP M 7 3개,SNP L 37 Indel, SNP L 8 Indel H 38 Indel, SNP M 9 Indel, SNP M 39 SNP H 10 1개,Indel H 40 Indel, SNP L 11 1개,Indel H 41 Indel H 12 4개,SNP H 42 Indel H 13 3개,SNP L 43 SNP L 14 Indel, SNP L 44 Indel, SNP L 15 Indel H 45 Indel H 16 SNP H 46 Indel L 17 1개 Indel H 47 2개,SNP H 18 Indel, SNP L 48 SNP H 19 SNP L 49 Indel, SNP L 20 Indel, SNP H 50 1개,SNP H 21 SNP H 51 Indel L 22 2개,SNP L 52 Indel, SNP M 23 1개,SNP L 53 SNP M 24 SNP L 54 SNP H 25 SNP M 55 SNP M 26 SNP M 56 Indel, SNP L 27 SNP M 57 1개,SNP H 28 Indel, SNP M 58 1개,SNP H 29 SNP L 59 Indel, SNP M 30 SNP H 60 SNP M HRM 분석 : L(1~2 groups), M(3~6 groups), H(7~ groups). - 92 -
- 93 -
μ μ - 94 -
- 95 -
- 96 -
- 97 -
- 98 -
- 99 -
- 100 -
μ μ - 101 -
- 102 -
α - 103 -
- 104 -
- 105 -
- 106 -
ccgctaccgatgtaatcgtaaacgacaataataagaacgaccttgtttccgtcaataaca aagacgatgttgccgctggttatgcttctgcggatgatgctgttcccaacgcccgaggga accgcgaacgtgagcgcaaacgaggtttgatcggcatcggatctccgatcgcctttgatt tctgtctctcttattgattcgttttaatttcttctagtttctgattctttttttgcctcc tgatttctttagattcaattttcgatctatttaatttagtagagtagctccgttttatga tcggaacgtacggaaagaaactagagtttggcttttgaatttctgtgtttgtgttgataa aataggtgtgccttggactgaggaagaacacaagctgtttttaattggactacaacaagt aggaaagggggattggagaggaatctctaggaactttgttaagactcgcactccaactca ggtcgctagccacgctcagaagtactttctccgccggagcaatcttaatcgccgtcgccg tagatctagtcttttcgatatcacgacggacacggtaactcactaatactttttctaatc aattactatatgctatttgtttgattgttgaatctgattacggaatagggatcttaatcc ctaacttttcaacaatcaagtttaacttggttgtcaattaggactcaactaattaggttt gtgattgacaaaatccatgatgaattactctctatgatgatggatctaagtttctatttc agtcttacattttcttaggcagacgttgacctttaaaagaccaaatgcttaaatgatttc atatttctagcttctgctctttaattgaccaataaaagacttgacttatgcatctcaaaa tttctatcatgtcgctcaccaacccccaaaagtttgaatttcaagatgaactctgatttt tatttctttttctttgcttttgttaggtcacggctgctccaatggttgaagaacccttgc agcgtaaagaaattccctctcaatcccattccttcactccatcaaccttacctgaaattt ctaaaaacaacgcaatcccaataatacaaactttaccagtttcatttgggccaacaccat tagccaccccaaccaagaatctaatagaaaactgtggtcctagagaagtaaacacagaaa atgatggatcactaaagctcgccctttctgattcgatcttttcctctaatcaaatacgaa atgaatcaccatcatcatcatcatcatcagctttatcccttcgcctttccttaacatctg accaaagagaaacttcatcaagacattcaactttccaacccatcccaagtttcaataatg gtgatgggatcataagtgctgcataatgaaaaatttacagatctgagaggaagaaagaag aagaagaagaagaagaaattattaacaattagagacgacagaagttattaggtgactgaa tctgatctgatccaatcatgttttttgatggcatctatgtaaaatctagattcttcttga tgatttgctgttgttgtcggtagcgg - 107 -
- 108 -
- 109 -
- 110 -
- 111 -
- 112 -
- 113 -
- 114 -
- 115 -
- 116 -
- 117 -
- 118 -
- 119 -
- 120 -
- 121 -
- 122 -
- 123 -
- 124 -
- 125 -
- 126 -
- 127 -