2011-2012 아이엠텍취급제품 종합안내서 용출기및주변장치 잒류오염물질전처리장비 자동적정장치 PROTEIN 회수장치 LC-FTIR 질소발생기 초순수제조장치 수분측정장치 HALO / SEPAX 컬럼 GC-FTIR
다국적기업에서얻은싞뢰 이제는국내제약사와함께합니다 Dissolution System DS-8000 1. USP 및모든국제약전에충족하는용출시스템 샘플링시에만작동하는자동샘플링시스템 Basket/Paddle 에동일핚 Mono Shaft 사용 2. GLP 규정에적합한시스템 Validation software (RPM, Temp, Sampling Volume & Replacement Volume) Evaluation Software 용도에맞는 Report Format DS-14000 3. 정확한용출율을보장하는시스템 원뿔형베셀덮게 자동알약동시주입기 최대 24개의샘플링포인트 4. Drug 제형과특징에맞는솔루션제공 Clean Function Fast Release Drug 용출을위핚솔루션 5. 알람과경고기능 적정온도알람 Wake-Up 기능 과열방지경고 Error 경고 Physical Testing System Tap Density Tablet Disintegration Tablet Friability Tablet Hardness
주요 특장점 Intelligent Sampling System USP, EP, BP & JP 기준에 적합 Media의 증발을 막아주는 원뿔형 덮게 Validation Software, Evaluation Software Tablet Dispensing System Basket/Paddle 교환이 편리한 Mono Shaft Automatic Filtration System & Piston Pump USP 기준 37±0.5 에서 준비완료 알람 Fast Release Drug을 위한 용출 방법 ±0.1 의 정밀한 온도조절 코팅된 알약을 위한 Clean Function 교차 오염을 방지하는 Rinsing Function 16 x 6(108 LC Vials)/24 x 6(144 Test Tubes) Tray 최장 720시간 연속 용출 가능 최대 24개 샘플링 포인트 사용
식품홖경유해물질을자동화된시료전처리장비로해결 - 30 분이내추출, 정제, 농축까지 PLE PRESSURIZED LIQUID EXTRACTION - Extraction Power Prep Multicolumn- Clean Up / Concentration TRP Total Rapid Prep - Extract / Cleanup / Concentrate
주요 특장점 초임계 상태에서 시료 추출 교차오염 방지를 위한 완전한 모듈화 GCMS / LCMS 용 바이알에 곧장 농축 다양한 시료 저장 용량 : 최대 250 추출 중 셀의 온도와 압력을 프로그래밍 추출 온도와 압력 자동 모니터링 자동화된 문서 기능 추출도중 Method 수동 조작 가능 동시에 자동 추출, 정제, 농축을 실시 3개 샘플 30분 이내 추출 SPE Solid Phase Extraction
Auto Titrator 주요특장점 뷰렛과실린더부피자동인식기능 3 가지표준적정모드제공 1 Incremental 2 Equilibrium 3 Cut-off by ph 시료의양에맞는다양핚뷰렛크기선정 (1/5/10/25) 선택적인첨가속도조젃기능 리포트포맷및결과재계산기능 - %, ppm, mg, KOH/g, G/L, Mol/L, mg/l Magnetic stirrer 혹은 Vortex stirrer 중선택사용 적정중측정된값 (mv, ph) 및첨가양, EP 값등모니터링 고객예상 End Point 9 개까지검증
KAFI KF Titrator 주요특장점 반응조내의습기자동모니터링 및자동중성화 다양핚응용에맞는 6 종류의베셀 제공 50 개분석방법저장 10 개결과재프로그래밍기능 액세서리 mg/ml, %, ppm, mg/g & 온라인 leak - 속도결과리포트기능 통계결과리포트기능 USP, EP, BP, JIS, BS, ASTM & DIN 기준에적합 GLP 기준에적합 Visual Melting Range Apparatus UV-VIS Spectrophotometer
Top-down, Bottom-up proteomics 에서획기적인전처리기술 GPR-800 (Gel Protein Recovery system) Features 효율적추출을위핚고급마이크로유체칩설계 8채널병렬처리 : 8개를동시에 20분안에추출 독립적으로분리된단백질 In-solution digestion을위핚젂처리 선택적인단백질추출후즉시 MS 분석가능 Microfluidic chip design for electroelution (A), and the protein recovery process (B). Fluid flow in the microfluidic chip (A), images of chip protein before and after electroelution (B). The Coomassielabeled protein is seen in the gel-loading reservoir
Sample prep (Application) Kits Catalog Number Kit name Kit Contents* Application GPR-050 GPR LC-MS Top-Down Prep Kit, Low MW C8 GPR Spin Tips Prep Kit GPR-055 C8 Spin Tips Prep Kit GPR MALDI Intact Mass CHCA Ultrapure MALDI Matrix Prep Kit, Low MW C8 LithTips Prep Kit GPR-060 GPR LC-MS Bottom-Up C8 GPR SpinTips Prep Kit Prep Kit, Low MW Trypsin Digestion Kit C8 Spin Tips Prep Kit GPR 065 GPR MALDI Bottom-Up CHCA Ultrapure MALDI Matrix Prep Kit, Low MW Trypsin Digestion Kit C18 LithTips Prep Kit * All Application Kits contain a surfactant degradation reagent Mass measurements and Top-Down analysis of intact proteins by LC-MS Mass measurements of intact proteins by MALDI Bottom-up analysis of post-gpr extracted proteins by LC-MS Bottom-up analysis of post-gpr extracted proteins by MALDI Other Products Facet TM Columns for ESI MS MALDI Sample Prep kit Protein Standards for MS ProteaGels Products
Manufacture by System Instruments Co., Ltd 일본시장점유율 1 위의 47 db 질소발생기 Nitrogen Generator for LC-MS/MS ( 일체형 ) Model 24F (30F) Features LC-MS/MS보다작은소음 (47 db) 실험실테이블밑의작은공갂에설치가능 분당 24 L N 2 gas 생산및 APCI 사용 내부배출기능 높은내구성을가짂압축기탑재 24F 30F 사용용도 일반적인 LC-MS UPLC+LC-MS Nitrogen Flow Rate (Purity) 4 L/min : 99.9% 22 L/min : 97.0% 13 L/min : 99.0% 30 L/min : 95.0% 24 L/min : 97.0% (Max.) Size (mm) Noise 485(W)/740(D)/680(H) 47 db Model 07 E-SDA (For Applied Biosystem MS) N 2 Gas 유량 : 6 L/min 순도 : 99.5% 이상압력 : 0.38-0.42 MPa (55-60 psi) Zero Air 유량 : 22 L/min 이슬점 : -40 압력 : 0.69-0.72 MPa (100-105 psi) Air 유량 : 6.4 L/min 압력 : 0.42 MPa (60 psi)
Nitrogen Generator for LC-MS/MS ( 분리형 ) 12EC 20EC Nitrogen Flow Rate (Purity) 4 L/min : 99.9% 9 L/min : 99.9% 13 L/min : 99.0% 26 L/min : 99.0% 25 L/min : 97.0% 51 L/min : 97.0% 30 L/min : 96.0% 63 L/min : 96.0% Size (mm) 200(W)/200(D)/780(H) 200(W)/150(D)/1300(H) Compressor SLP-221EBD-S SLP-371EBD-S Other Model : 30EC(94 L/min), 50EC(100 L/min, 200EC (360 L/min) Compressor (oil-free scroll type) for 12EC or 20EC SLP-221EBD-S (12EC) SLP-371EBD-S (20EC) Air Flow Rate 215 L/min 345 L/min 35 L 20 L Size (mm) 453(W)/682(D)/975(H) 545(W)/622(D)/1158(H) Noise 50 db Nitrogen Generator for ELSD 05B O5BL N2 Gas Pressure 0.5 MPa 0.7 MPa (Max.) 1 L/min : 99.9% 1 L/min : 99.9% 5 L/min : 99.9% 5 L/min : 99.0% 15 L/min : 96.5% Size (mm) Noise 300(W)/526(D)/481(H) 45 db
지멘즈가만들면초순수제조기도다릅니다. LaboStar UltrapureWater Systems Features 최고품질, 빠른준비시갂 무소음모드 빠르고쉬운소독 젂도도모니터링 쉬운모듈교체 LaboStar SystemModel 1-DI/2-DI 1-UV/2-UV 3-DI/4-DI 3-UV/4-UV Ultrapurewater specifications Output* up to l/min 1.5 1.5 1.5 1.5 Conductivity at 25 C μs/cm 0.055 0.055 0.055 0.055 Resistivity at 25 C MΩ-cm 18.2 18.2 18.2 18.2 TOC ppb 5 ~ 10 1 ~ 5 5 ~ 10 1 ~ 5 Bacteria cfu/ml < 1 < 1 < 1 < 1 Endotoxins with use of filter EU/ml < 0.001 < 0.001 < 0.001 < 0.001 Particles > 0.2 μm per ml < 1 < 1 < 1 < 1 Feedwater specifications Feedwater pressure bar 0 ~ 6 0 ~ 6 - - Feed conductivity μs/cm < 20 < 20 < 20 < 20 TOC ppb < 50 < 50 < 50 < 50 Silica ppm 2 2 2 2 Temperature C 5 ~ 35 5 ~ 35 5 ~ 35 5 ~ 35 Flowsheet of LaboStar 2-DI/UV LaboStar with Biofilter LaboStar TWF UltrapureWater Systems Features 급수에서바로초순수생산 급수와바로연결 7 L Tank 연결 Pre-treatment 모듈및젂도도모니터링 LaboStar 3/7 SystemModel DI UV Ultrapurewater specifications Output* up to l/min 1.2 1.2 Permeate rate l/h 3/7 3/7 Conductivity at 25 C μs/cm 0.055 0.055 Resistivity at 25 C MΩ-cm 18.2 18.2 TOC ppb 5 ~ 10 1 ~ 5 Bacteria cfu/ml < 1 < 1 Endotoxins with use of filter EU/ml < 0.001 < 0.001 Particles > 0.2 μm per ml < 1 < 1 Conductivity of inputwater is reduced by 98% Feedwater specification Feedwater pressure bar 3 ~ 5 3 ~ 5 Feed conductivity μs/cm < 1400 < 1400 Temperature C 5 ~ 35 5 ~ 35 Flowsheet of LaboStar 2-DI/UV
Pure Purity - UltraClear Systems Features TOC 모니터링 ( 옵션 ), UV 조사감도조젃, 프로그램가능핚용량조젃기능기본내장 0.1 um 멸균필터내장 총유기탄소 1ppb 이하 (UV) Endotoxin 제거 (plus) 디스펜서길이연장가능및 data저장가능 TOC : < 1 ppb Ultra Clear SystemModel Ultrapurewater specifications - UV plus UV plus UV TM UV plus TM Output* up to l/min 2 2 2 2 2 2 Conductivity at 25 C μs/cm 0.055 0.055 0.055 0.055 0.055 0.055 Resistivity at 25 C MΩ-cm 18.2 18.2 18.2 18.218.2 18.2 18.2 TOC ppb 5 ~ 10 < 1 5 ~ 10 < 1 < 1 < 1 DNase, RNase, DNA free free Bacteria cfu/ml < 1 < 1 < 1 < 1 < 1 < 1 Endotoxins with use of filter EU/ml - - - < 0.001 - < 0.001 Particles > 0.1 μm per ml < 1 < 1 < 1 < 1 < 1 < 1 Feedwater specification Feedwater pressure : 0.1 ~ 5 bar Feed conductivity : < 20 μs/cm TOC < 50 ppb Flowsheet ofultra Clear UV Plus system UltraClear Systems Direct Purity TWF Features One system에서이온이제거된초순수생산 급수직접연결 0.1 um 멸균필터내장 총유기탄소 1 ppb 이하 (UV) Endotoxin 제거 (plus) 디스펜서길이연장가능및 data저장가능 El-Ion CEDI cell option Ultra Clear TWF Models Ultrapurewater specifications - UV plus UV plus UV TM UV plus TM Output* up to l/min 1.8 1.8 1.8 1.8 1.8 21.8 Conductivity at 25 C μs/cm 0.055 0.055 0.055 0.055 0.055 0.055 Resistivity at 25 C MΩ-cm 18.2 18.2 18.2 18.218.2 18.2 18.2 TOC ppb 5 ~ 10 < 1 5 ~ 10 < 1 < 1 < 1 DNase, RNase, DNA free free Bacteria cfu/ml < 1 < 1 < 1 < 1 < 1 < 1 Endotoxins with use of filter EU/ml - - < 0.001 < 0.001 - < 0.001 Particles > 0.1 μm per ml < 1 < 1 < 1 < 1 < 1 < 1 Feedwater pressure : 0 ~ 5 bar Colloid index SDI : < 3 Feedwater Free Chlorine : < 0.5 mg/l Fe : < 0.1 mg/l specification Feed conductivity : < 2000 μs/cm Flowsheet of Ultra Clear TWF UV plus TM system
일반 HPLC 에서 UPLC 의분리능과감도, 속도구현 About Halo Column Features 최고순도의 Type B silica 사용 1.7 μm soild core 입자와 0.5 μm 다공성 silica층 최대압력 9000 psi (600 bar) The shorter diffusion path of HALO reduces axial dispersion The shorter diffusion path of HALO particles reduces axial dispersion of solutes and minimizes peak broadening. Because of the shorter diffusion path, the performance advantages of HALO become even more apparent when separating larger solute molecules and operating at faster mobile phase flow rates Scanning electron microscope (SEM) photograph of HALO particles HALO columns are designed for hyper-fast UHPLC separations Comparison of van Deemter plots Comparison of column back pressure HALO UHPLC columns deliver more separating power
Halo Reverse Phase Column Stability testing of a HALO UHPLC column Structure of bonded phase of HALO RP-Amide Structure of bonded phase of HALO Phenyl-Hexyl Halo Peptide ES C18 column Acid Hydrolysis of the Siloxane Bond (other peptide column) Extra Stable (ES) Bonded Phase Stability testing of HALO Peptide ES-C18 column High Resolution Separation Using HALO Peptide ES-C18
Sepax 만의 Column Chemistry 기술 HILIC (Hydrophilic Interaction Chromatography) Phase Features HILIC Column Cross References by Manufactures 5종의서로다른작용기 ph 안젂성 : 1.5-8.0 분석 sample : polar pharmaceuticals, peptides, amino acid 등 Chemical structures of HILIC phases Separation of Nucleosides Selection of Sepax HILIC columns Separation of nucleosides by various HILIC columns (5 μm, 4.6x150 mm). Mobile phase: 90/10(V/V) = ACN/10mMammonium acetate in H2O. Flow rate: 1.0 ml/min. UV 254 nm. Ambient Temperature. Sample: 1. Uracil, 2. Adenosine, 3. Uridine, 4. Cytidine, 5. Guanosine. HighCapacity proteomix IonExchange Phases - Proteomix 이온교홖 column은 oligonucleotides, carbohydrates 와 peptides의높은 resolution, efficiency와 recovery 분리를위해특별하게디자인 Features 충짂입자크기는 1.7, 3, 5 및 10 um선택가능 높은압력에서분석가능 : 각각 4000, 6000, 8000, 12000 psi 가능 넓은 ph범위 : 1 ~ 12 분석가능 sample : peptides, carbohydrates and glycans, proteins, polynucleotides, cell lysates 등 Separation of 2-AA (anthranilic acid) labeled N-linked oligosaccharide (profiling of an IgG1 sample). A: 2.5% (v/v) acetic acid, 0.5% TEA in H2O; B: 0.5% acetic acid in ACN. Gradient 0-100%B (60 min); 0.3 ml/min. Fluorescence detection: Ex/Em=360/425nm Chemical compositions of Proteomix Chemical structure
Zenix TM Size Exclusion Chromatography Phases - Highest Efficiency and Resolution Size Exclusion Separation Features 충짂입자크기 3 um 공극사이즈선택 : 100, 150 및 300 Å 높은용적율 생물학적홗성이변하지않은상태로높은단백질회수 고농도의염이나적은용량에도높은안정성 최상의생물학적분자의분리및분석가능 : proteins, nucleic acid 등가능 최상의천연고분자의분리및분석가능 : polysaccharides, synthetic polymers 등 Separation of protein mixture A by Zenix SEC-150 and300 columns with 7.8 mm ID. Carbomix Column Chemical compositions of Proteomix Separation of carbohydrates and sugar alcohols on a Carbomix Pb-NP5 column Chromatograms of organic acids on a Carbomix H-NP5 column after the column being continuously flushed with 5 mm H 2 SO 4 at 55. Selection of Ionic-form for Different Applications Phase H-NP Ca-NP Pb-NP K-NP Na-NP Support Non-porous PS/DVB Particle size (μm) 5, 10 5, 10 5, 10 5, 10 5, 10 Crosslinkage 5%, 8%, 10% 5%, 8%, 10% 5%, 8%, 10% 5%, 8%, 10% 5%, 8%, 10% Stationary phase -SO 3 H -(SO 3 ) 2 Ca -(SO 3 ) 2 Pb -SO 3 K -SO 3 Na ph stability 1 3 5 9 5 9 5 9 5 9 Typical mobile phase Typical flow rate Maximum temperature 2.5 mm H 2 SO 4 H 2 O H 2 O H 2 O H 2 O or 0.1% H 3 PO 4 7.8x300mm : 0.4-0.8 ml/min 4.6x300mm : 0.1-0.30 ml/min 85 o C
완벽한정성 & 정량분석?? 이제는 LC-FTIR 로!! 특징 DiscovIR-LC (For use with HPLC & GPC) 구조이성질체와기하이성질체확인 LC/MS에서구분이어려운동일질량화합물의작용기스펙트럼을이용핚정량및정성분석 10,000개이상화합물 library search 및구축 이동상으로 100% 물사용가능 LC-FTIR 분석과정 Step 1. Solvent Remove Step 2. Sample Detection Step 3. Data Analysis LC-FTIR Chromatography 의주요장점 동일분자량이성질체의특정작용기차이확인을통한분석 정량분석을위한재현성 & 직선성 MS 분석 치홖기에붙어있는당화합물의종류 ( 예 glucose, galactose) 를 MS 분석만으로확인어려움 D-glucose D-galactose IR 분석 Spectrum 차이가나타남 정확한 Library Search LC-FTIR 응용 차추출물 ( 구조이성질체분석 ) Band 크로마토그램을이용핚 선택적검출 ( 작용기선택검출 )
완벽한정성 & 정량분석?? 이제는 GC-FTIR 로!! 특징 DiscovIR-GC (For use with Gas Chromatography) 구조이성질체와기하이성질체확인 GC-MS에서구분이어려운동일질량화합물의작용기스펙트럼을이용핚정량및정성분석 10,000개이상화합물 library search 및구축 GC-FTIR 분석과정 Step 1. Sample Detection Step 2. Data Analysis Step 3. Library Search GC-FTIR chromatography 적용분야 향수에서특정작용기그룹탐색 Fatty Acid Methyl Ester (FAME) 분석 부분입체이성질체분석 유사구조분석 구조이성질체분석