(Establishment and Management of Proteomics Core Facility)

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1 (Establishment and Management of Proteomics Core Facility)

13 1 sample running on SDS-PAGE Gel (10well) D size marker Buffer(20X, 500ml) Staining kit

14 2-DE Gel construction for 1 sample 2D extraction buffer cm strip Acry/Bis solution Buffer Staining kit MALDI-MS/MS analysis for 1 band(spot) in 1-D(2-D) gel after in-gel digestion Trypsin Tip Tube Matrix standard kit Zip-tip Source cleaning Plate cleaning kit AnchorChip 600/ LC-MS/MS analysis for 1 sample in-gel digested Trypsin Tip Tube D gel running Zip-tip ACN Water Analutic column Trap column Spray tip LC-MS Standard

- 국내주요단백질체연구기관의장비보유현황 (2007.9.) 1) KIST Molecular Proteomics lab.

15 - 국내주요단백질체연구기관의장비보유현황 ( ) 1) KIST Molecular Proteomics lab. 장비 MALDI-TOF MALDI-TOF/TOF Triple quadruple Ion Trap Q-TOF FTICR 대수 ) 기초과학연구소 장비 MALDI-TOF MALDI-TOF/TOF Tripole quadrupole Ion Trap Q-TOF FTICR 대수 ) 삼성생명과학연구소 장비 MALDI-TOF MALDI-TOF/TOF Tripole quadrupole Ion Trap Q-TOF FTICR 대수 ) 연세프로테옴센터 장비 MALDI-TOF MALDI-TOF/TOF Tripole quadrupole Ion Trap Q-TOF FTICR 대수 ) 국립암센터 장비 MALDI-TOF MALDI-TOF/TOF Tripole quadrupole Ion Trap Q-TOF FTICR 대수 국내단백질체연구소장비보유현황조사에기반, 최신의 Ion Trap MS 도입 ( ) 그림 6. 새로도입된 LTQ-XL IonTrap MS

Sample Name y-17_1 Sample Name y-17_2 Reference P (pro) Score Scan(s) Peptide P (pep) XC Gene_Symbol=ERP29 Endoplasmic reticulum protein ERp29 precursor 4.35E-05 20.20 3550 K.GALPLDTVTFYK.V 9.

34 Sample Name y-17_1 Sample Name y-17_2 Reference P (pro) Score Scan(s) Peptide P (pep) XC Gene_Symbol=ERP29 Endoplasmic reticulum protein ERp29 precursor 4.35E K.GALPLDTVTFYK.V 9.33E K.GALPLDTVTFYK.V 4.35E K.SLNILTAFQK.K 2.15E K.SLNILTAFQK.K 2.06E Reference P (pro) Score Scan(s) Peptide P (pep) XC Gene_Symbol=PHB Prohibitin 1.31E R.FDAGELITQR.E 2.11E R.FDAGELITQR.E 1.31E R.VLPSITTEILK.S 8.18E R.VLPSITTEILK.S 3.78E Sample Name y-17_3 Reference P (pro) Score Scan(s) Peptide P (pep) XC Gene_Symbol=HSPB1 Heat shock protein beta E R.LFDQAFGLPR.L 4.09E

35 ➅ Ast_01 Reference P (pro) Score 1 Gene_Symbol=ALB Uncharacterized protein ALB 1.17E Gene_Symbol=PEPD Xaa-Pro dipeptidase 2.95E Gene_Symbol=SERPING1 Plasma protease C1 inhibitor precursor 4.48E Gene_Symbol=BGN Biglycan precursor 1.14E Gene_Symbol=ACOT9 acyl-coenzyme A thioesterase 2, mitochondrial isoform b Gene_Symbol=DKFZp686D0972 hypothetical protein LOC E E Gene_Symbol=LOC similar to cytoplasmic beta-actin 3.14E Gene_Symbol=SRRD Hemochromatosis protein (Fragment) 3.58E Gene_Symbol=CFB Isoform 1 of Complement factor B precursor (Fragment) 6.99E Ast_02 Reference P (pro) Score 1 Gene_Symbol=COL3A1 Isoform 1 of Collagen alpha-1(iii) chain precursor 4.53E Gene_Symbol=ALB Uncharacterized protein ALB 2.35E Gene_Symbol=BGN Biglycan precursor 5.57E Gene_Symbol=MFSD5 Isoform 2 of Major facilitator superfamily domain-containing protein E

36 ➆ Sample Name cox2_1 Sample Name cox2_2 Reference P (pro) Score Scan(s) Peptide P (pep) XC Gene_Symbol=PYGM Glycogen phosphorylase, 9.87E muscle form 2416 R.VAAAFPGDVDR.L 9.87E Reference P (pro) Score Scan(s) Peptide P (pep) XC Gene_Symbol=MYH9 Myosin E R.NAEQYKDQADKASTR.L 6.43E R.TEM*EDLM*SSKDDVGK.S 1.44E K.RQLEEAEEEAQR.A 4.92E R.QLEEAEEEAQR.A 3.11E Q.LEEEEEAKHNLEK.Q 1.26E R.ELEDATETADAM*NR.E 1.10E R.ALEEAM*EQKAELER.L 8.61E K.VKPLLQVSR.Q 3.80E K.VKLQEMEGTVK.S 4.30E R.TVGQLYKEQLAK.L 5.09E R.QLEEAEEEAQR.A 5.42E R.QEEEMM*AKEEELVK.V 2.94E R.QLEEAEEEAQR.A 3.05E T.LENERGELANEVK.V 6.51E K.ASITALEAK.I 1.38E K.YKASITALEAK.I 3.33E K.ASITALEAK.I 8.64E

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