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1 ; (11), WHO The Morphology of Liver through the Electron Microscope (11) Tae Joong Sohn, M.D., Ph.D. Emeritus Prof essor, School of Medicine, Kyungpook National University, Head, WHO Collaborating Centre f or Research and Training in Diagnostic Electron Microscopy, Taegu, Korea ( ) ( 6 3,4) (nucleus ) (, endoplasmic reticulum, ER) 1992 < > < endoplasmic reticulum > <,,, >. < ( )> (, 1926 ) ER,. (A ) (I) 9. A. (endoplasm and ectoplasm) 2. (gel) (sol). (ect oplasm ), (endoplasm ).. endoplasmic reticulum endoplasm. B.? (What is endoplasmic reticulum?) 1945 (Rodney Robert Porter, 1917 ), µm (lace- like reticulum ).,. endoplasm lace- like reticulum < endoplasmic reticulum, ER>. ER 1952., 115

The Korean Journal of Hepatology : Vol. 7. No. 1. 2001 F ig ure 1.

2 The Korean Journal of Hepatology : Vol. 7. No F ig ure 1. Scanning electron microscopic image of liver from a mouse showing a junctional area of ser and rer. ( 44,200) 116

3 Tae Joong Sohn. The Morphology of Liver through the Electron Microscope (11) Fig ure 2. Nobel- prize winners 1974 in appreciation of searching structures and functions of the cell organelles using electron microscope (from left, A. Claude, C.R. de Duve and G.E Palade). ER, ER. ER Sjöstrand ER cytomembrane.,, ER (George Emil Palade, 1912 ) nm (ribosome) rough - surfaced variety of ER, smooth - surfaced variety of ER. rough - surfaced ER rer, smooth - surfaced ER ser ( 1)., (Albert Claude, ),, (m icrosom e) Siekevitz ,, Sjöstrand 3. -, - - cytomembrane.,, ER cytomembrane., - cytomembrane rer, - cytomembrane, - cytomembrane 117

4 Schulz Paola cytomembrane,., endoplasmic reticulum, Golgi membrane, plasma membrane. C. (endoplasmic reticulum and Nobel prizes) ER ER < > 1972 (Gerald Mourice Edelman, 1929 ). ER 2 ER (lysosome) (Christian Rene de Duve, 1917 ) 3 < > ( 2). < > , (T EM ) 0.1 nm, (SEM ) 0.5 nm. ( nm ). ( ) (HRT EM ) (ZrO2) (Zr ) (O) (structural image) (image) HRT EM image. BC 4C (Democritus, 460? 370? BC),, 20C ( ), 2,500..,.,,,,,,,, ( ) < > (visualize)., (JD Bernal) 118

5 . (11) T able 1. Relationship between Cytoplasmic Matrix and ER Light microscopic level Cytoplasmic Martix Ergastoplasm 20C., ( )..., ( ) ( ), ( ), ( ), ( ), ( ), ( ), ( ) ( ).... D. (relationship between cytoplasmic matrix and endoplasmic reticulum),. Electron microscopic level rer ER ser annulate lamellae ribosome, polysome, microtubule, microfilament etc matrix lamellar structured rer (matrix ) ( 1).. H - E.. (basophilic or chromidial substance). < ergos working > (erg astoplasm ). ( )....,,,,. 119

6 The Korean Journal of Hepatology : Vol. 7. No (hom og eneou s and structureless).. E. (ribosomes and polysomes or polyribosomes) nm (ribosome).,,,. ( ) ( ), ( ), ( ) 2 ( ) ( 3, 4). ( ), 2 (subunit ). (ribonucleoprotein complex ) RNP (RNP particle). (RNA ). 70S (sedimentation coefficient ), 80S 80S, 70S.. 80S 60S, 40S, RNA. 10. (poly some) (polyribosome).,. RNA (mrna ). mrna 3 nm (mrna filament ). mrna filament. mrna. ( 64,000) mrna, 20 ( 3).,, 2. (mem - brane- bound form attached ribosome), (free form free ribosome)..,.,. F. (ultrastructure of endoplasmic reticulum) 5 6 nm ( ),,,.,, (compartment ).. 20% 120

7 Tae Joong Sohn. The Morphology of Liver through the Electron Microscope (11) 65%..,, 2. (rough - surfaced ER, rer agranular ER), (smooth - surfaced ER, ser agranular ER) ( 5, 6)..,.,. (homogenate).,,..... (rer) ( 3, 4),,,. (ergastoplasm ),.,... (surface image) (glu cose- 6- phosphat ase) (lead method). (substrate) , (matrix ) - 6-,,, ,, ( ). (s ER) ( 5, 6).,.,,,, (Clara ) ( ),,..,,.,, 12 1

8 ( ).. G. (rough- surfaced ER and protein synthesis)., (exocytosis )., (signal hypothesis). mrna (signal codon ), (initiation codon, AUG ) 3 '.. (signal sequence),... (signal peptidase). (detachment factor ).. (ly sosome),. ( )., (core carbohydrate)..,..,. H. (function of smooth- surfaced ER) 122

9 . (11),,,,,,,. (glycogen metabolism ) (st eroid h orm on e metabolism ).,,..,. (Clara cell).., P (cytochrome P - 450).. (parietal cell), (intracellular canaliculus).... I. (special forms of endoplasmic reticulum) (Nissl s bodies)..., myeloid body,.. (sarcoplasmic reticulum ).. 1., ( ). (I). :, ( ).. :, ( ).. :, ( ).. :, :, :, , ( ). ( )- -. :, , ( ).. :, Alberts B, et al: Essential cell biology - An introduction to the molecular biology of the cell -. New York: Garland publishing, Inc :, :,

The Korean Journal of Hepatology : Vol. 7. No. 1. 2001 Fig ure 3.

10 The Korean Journal of Hepatology : Vol. 7. No Fig ure 3. Scanning electron microscopic image of liver from a mouse showing ribosomes attached on membrane of rer which are consisted of small and large sub-particles. ( 208,000) 124

11 Tae Joong Sohn. The Morphology of Liver through the Electron Microscope (11) Fig ure 4. Scanning electron microscopic image of liver from a mouse showing ribosomes attached on membrane of rer which are forming small group with ribosomes called polysome or polyribosome and mrna filament passing between small and large sub- particles of ribosomes(cited from NHK enterprise). ( 82,000) 125

12 Fig ure 5. Scanning electron microscopic image of liver from a mouse showing ser, rer, Golgi apparatus and mitochondria. ( 36,000) 126

13 . (11) F ig ure 6. Scanning electron microscopic image of liver from a mouse showing ser. ( 104,000) 127

The Korean Journal of Hepatology : Vol. 7. No. 1. 2001 Fig ure 7.

14 The Korean Journal of Hepatology : Vol. 7. No Fig ure 7. Transmission electron microscopic image of liver from a 2 years old, male patient with hepatic storage disease showing normal appearing hepatocytes containing numerous mitochondria, glycogen particles and well-developed rer. A whorled inclusion suggestive of sphingomyelin is also seen. ( 12,000) 128

15 Tae Joong Sohn. The Morphology of Liver through the Electron Microscope (11) Fig ure 8. Transmission electron microscopic image of liver from a 51 years old, male patient with alcoholic hepatitis showing mild degenerative changes of mitochondria, dilatation of bile canaliculus and degeneration of junctional complex. Deposition of numerous lipofuscin like materials in cytoplasm are also noted. ( 9,500) 129

16 Fig ure 9. Transmission electron microscopic image of liver from a 55 years old, male patient with chronic active hepatitis showing degeneration of mitochondria, increase of ser and increase of negative images of collagen bundles. A few lipid droplet are also noted. ( 12,600) 130

388 The Korean Journal of Hepatology : Vol. 6. No COMMENT 1. (dysplastic nodule) (adenomatous hyperplasia, AH), (macroregenerative nodule, MR

388 The Korean Journal of Hepatology : Vol. 6. No COMMENT 1. (dysplastic nodule) (adenomatous hyperplasia, AH), (macroregenerative nodule, MR 6 3 2000 ; 387-392 (3) Dysplastic Nodule Young Nyun Park, M.D., Chanil Park, M.D. Department of Pathology, Yonsei University College of Medicine BRIEF HISTORY 56. AST/ALT 72/73 IU/L, total bilirubin 0.7

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歯19-손태중.PDF