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16 CONTENTS SUMMARY (Korea) ⅰ SUMMARY (English) ⅴⅱ Contents (English) ⅹⅲ Contents (Korea) ⅹⅴ List of Tables ⅹⅴⅲ List of Figures ⅹⅹ Chapter 1. Introduction Section 1. Objectives and Contents Objectives Contents Scope Section 2. Annual objectives and contents Chapter 2. International technical development trends Section 1. Two-stage hybrid ASBF system Section 2. Isolation and mass production of ammonia-resistant anaerobic microorganism Section 3. Removal of nutrients and utilization of biomass as a biofertilizer Chapter 3. Development of two-stage hybrid ASBF system Section 1. Experimental set-up and method Characteristics of swine wastewaters Optimization of lab-scale anaerobic bioreactors Full-scale two-stage hybrid ASBF system Section 2. Results and discussion Characteristics of swine wastewaters Optimization of lab-scale anaerobic bioreactors Full-scale two-stage hybrid ASBF system
17 Section 3. Conclusions Chapter 4. Isolation and mass production of ammonia-resistant anaerobic microorganism Section 1. Isolation of ammonia-resistant anaerobic microorganism Section 2. Characterization and determination of growth conditions for ammonia-resistant anaerobic microorganisms Section 3. Influence of ammonia on anaerobic microorganism Section 4. Mass production of ammonia-resistant anaerobic microorganism Section 5. Conclusions Chapter 5. Removal of nutrients and utilization of biomass as a biofertilizer Section 1. Introduction Section 2. Experimental set-up and methods Section 3. Results and discussions Section 4. Conclusions Chapter 6. Technical achievements and contributions Chapter 7. Field application plans of the results Chapter 8. Technical information obtained during R&D Chapter 9. References
18 목 차 요약문 ⅰ SUMMARY ⅴⅱ 영문목차 ⅹⅲ 국문목차 ⅹⅴ 표 목차 ⅹⅴⅲ 그림 목차 ⅹⅹ 제 1 장 연구개발 과제의 개요 제 1 절 연구개발의 목표 및 내용 연구개발의 목표 연구개발 내용 및 범위 제 2 절 연차별 연구개발 내용 및 범위 제 2 장 국내 외 기술개발 현황 제 1 절 이단 혼합형 ASBF system에 대한 기술 제 2 절 암모니아 내성 혐기성 미생물의 분리 및 대량배양 제 3 절 생물학적 N,P 제거 및 균체 이용방안 제 3 장 이단 혼합형 혐기성 소화공정의 개발 제 1 절 실험장치 및 방법 축산폐수의 발생량과 특성 Lab-scale 혐기성 소화공정의 구성과 최적화 연구 가. 미디어 종류 및 깊이 그리고 이단화에 따른 소화공정의 최적화 나. 이단 혼합형 혐기성 소화공정의 최적화 Full-scale 이단 혼합형 ASBF system에 대한 연구 제 2 절 결과 및 고찰 축산폐수의 발생량과 특성 Lab-scale 혐기성 소화공정의 구성과 최적화 연구 가. 이단화와 filter media에 대한 영향 나. Lab-scale 이단 혼합형 ASBF 생물반응기 공정의 최적화 ) 인공폐수에 의한 고유기물 부하에서 이단 혼합형 ASBF
19 생물반응기의 성능 가) 혐기성 미생물의 환경조건 나) 유기물질 제거 효율 다) 혐기성 미생물에 대한 영양염류 라) Biogas production 마) 생물 반응기내 Biomass의 보유능력 ) Lab-scale 이단 혼합형 ASBF 생물반응기의 축산폐수에 응용 가) 혐기성 미생물의 환경조건 나) 축산폐수의 유기물질 제거 효율 다) 혐기성 미생물에 대한 영양염류 라) Biogas production 마) 생물반응기내 Biomass 보유능력 바) 이단 혼합형 ASBF 생물반응기의 유입수에 대한 성능비교 Full-scale 이단 혼합형 ASBF system 가. 암모니아 제거와 막분리 공정 나. Full-scale(실증용) 이단 혼합형 ASBF system ) 이단 혼합형 ASBF system의 설계 ) System의 제작 및 시운전 제 3 절 결론 제 4 장 암모니아 내성 혐기성미생물의 분리 및 대량배양 제 1 절 암모니아 내성 혐기성 미생물의 분리 실험장치 및 방법 분석방법 혐기성 미생물의 MCR 반응기에서의 적응 제 2 절 혐기성 미생물의 생리적 특성 및 생육조건 확립 정상상태에서 암모니아 농도에 따른 영향 혐기성 미생물의 생육조건 제 3 절 암모니아 내성 혐기성 미생물에 대한 독성/저해 영향 분석 제 4 절 암모니아 내성 혐기성 미생물의 대량 배양 제 5 절 결론
20 제 5 장 생물학적 N,P 제거 및 균체 이용방안 제 1 절 서론 제 2 절 실험 장치 및 방법 제 3 절 결과 및 고찰 제 4 절 결론 제 6 장 목표달성 및 관련 분야 기여도 제 7 장 연구개발 결과의 활용 계획 제 8 장 연구개발 과정에서 수집한 해외과학 기술정보 제 9 장 참고문헌
21 표 목차 (List of Table) Table 1-1. General characteristics of different types of wastewaters(ww) Table 1-2. Characteristics of swine wastewaters collected form 27 pig farms Table 1-3. Discharge limitations for public swine wastewater treatment plants Table 3-1. Characteristics of filter media used in filter bioreactors Table 3-2. Operational strategies for anaerobic bioreactors Table 3-3. Characteristics of swine wastewater used as an influent of filter bioreactors Table 3-4. Composition of nutrient/mineral/buffer(n/m/b) medium Table 3-5. Characteristics of swine wastewaters used as an influent of lab-scale two-stage ASBF bioreactors collected from A-San city's public swine wastewater treatment plant Table 3-6. Influent characteristics of two-stage ASBF-C bioreactor--25 Table 3-7. Influent characteristics of two-stage ASBF-SW bioreactor-25 Table 3-8. General characteristics of swine wastewaters sampled from 27 pig farmers during July May Table 3-9. Performance comparison of anaerobic bioreactors at the steady-state conditions Table Perfomance comparison of anaerobic bioreactors at the steady-state conditions Table Performance comparison of anaerobic bioreactors at the steady-state condition Table Monitoring 10 m 3 /d full-scale two stage hybrid ASBF system during the 2 months of start-up operation Table 4-1. Composition of Nutrient/Mineral/Buffer (N/M/B) medium Table 4-2. Comparison of operational parameters of MCR-1 and -2 after addition of ammonia to reactors before and after adaptation on ammonia
22 Table 4-3. Performance comparison of MCR-1 and -2 after addition of ammonia to reactors before and after adaptation on ammonia---86 Table 5-1. Effect of carbon source on nitrogenase activity of Nostoc HN601 isolated from rice field Table 5-2. Effect of carbon source on nitrogenase activity of Anabaena HA701 isolated from rice field Table 5-3. Effect of nitrogen source on nitrogenase activity of Nostoc HN601 isolated from rice field Table 5-4. Effect of nitrogen source on nitrogenase activity of Anabaena HA701 isolated from rice field Table 5-5. Comparison of total nitrogen and phosphorus content in cyanobacteria isolated from paddy soil Table 5-6. 논 토양에서 분리한 Anabaena sp. 와 Nostoc sp.의 Chlorophyll 함량의 경시적 변화 Table 5-7. 국내 논 토양에서 분리한 Anabaena와 Nostoc의 생육 배지의 인산농도 변화 Table 5-8. 국내 논 토양에서 분리한 Anabaena와 Nostoc의 생육 배지의 질산농도 변화 Table 5-9. Nostoc HN601의 생육에 미치는 ph의 영향 Table Nostoc HN601의 배지 인산제거에 미치는 ph의 영향 Table Nostoc HN601의 질소 제거에 미치는 ph의 영향 Table 미생물 처리에 따른 상추의 지상부 무게(g)와 상추의 잎 100cm2 당 무게 (g) Table 6-1. 세부과제별 목표 달성 내용 달성도
23 그림 목차 (List of Figures) Figure 1-1. Conceptual schematics of biological advanced treatment system and recovery system of treatment by-products Figure 3-1. Schematics of lab-scale anaerobic bioreactros Figure 3-2. Schematics of lab-scale two-stage ASBF bioreactors Figure 3-3. COD removal efficiency of lab-scale anaerobic bioreactor Figure 3-4. Effluent ph and alkalinity of lab-scale anaerobic bioreactors Figure 3-5. Effluent T-N of lab-scale anaerobic bioreactors Figure 3-6. Effluent T-P of lab-scale anaerobic bioreactors Figure 3-7. Effluent ph and alkalinity since the start-up operation-33 Figure 3-8. Effluent COD concentration since the start-up operation-34 Figure 3-9. Effluent COD removal efficiency since the start-up operation Figure Effluent VFAs concentration since the start-up operation Figure Steady-state COD removal efficiency at different OLRs--36 Figure Effluent TKN concentration since the start-up operation Figure Effluent T-Pconcentration since the start-up operation Figure Cumulative gas production & CH 4 composition since the start-up operation Figure Effluent TSS & VSS concentration since the start-up operation Figure Steady-state COD profile throughout bioreactor height at different OLRs Figure Effluent ph and Alkalinity since the start-up operation Figure Effluent COD concentration since the start-up operation
24 Figure Effluent COD removal efficiency since the start-up operation Figure Effluent VFAs concentration since the start-up operation Figure Steady-state COD removal efficiency at different OLRs--47 Figure Effluent TKN concentration since the start-up operation Figure Effluent T-P concentration since the start-up operation Figure Cumulative gas production & CH4 composition since the start-up operation Figure Effluent TSS/VSS concentration since the start-up operation Figure TCF-10 ultrafiltration system to fractionate organic matter in swine wastewaters based on size(molecular weight cut-off) Figure Size distributions based on molecular weight cut-off of organic matter in swine wastewaters of two-stage ASBF bioreactors Figure Conceptual schematics of two-stage ASBF system with ammonia removal pretreatment and membrane process for effluent polishing Figure Flow sheet of two-stage ASBF system Figure PID of two-stage ASBF system Figure Electrical diagram of two-stage ASBF system Figure Design of V-notch of two-stage ASBF system Figure Design of flocculation tank of two-stage ASBF system---61 Figure Design of precipitation tank of two-stage ASBF system--62 Figure Design of heat exchanger of two-stage ASBF system Figure Design of 1st anaerobic bioreactor of two-stage ASBF system Figure Design of 2nd anaerobic bioreactor of two-stage ASBF system Figure Design of holding tank for MF of two-stage ASBF system
25 Figure Design of holding tank for NF of two-stage ASBF system Figure Design of nanofiltration of full scale two-stage ASBF system Figure View of pig farm and two-stage ASBF system in containment-70 Figure Front part of two-stage ASBF system (From right, reaction tank with 2 chemical tanks with pumps, sedimentation tank, heater exchanger) Figure Detailed pictures of front part of two-stage ASBF system (From bottom to top : reaction tank with two chemical tanks with pump, sedimentation tank, and pre heater tank) Figure Two-stage ASBF bioreactors with two mono pumps and control system Figure NF membrane system with control panel Figure 4-1. Configuration of master culture reactor(mcr) Figure 4-2. Patterns of COD removal and gas production during adaptation on batch operation of MCR Figure 4-3. Patterns of COD removal and gas production during adaptation on batch operation of MCR Figure 4-4. Patterns of COD removal and gas production after addition of ammonia to MCR at steady-state operations Figure 4-5. Mixed Bioreactor(1 m 3 ) made from FRP Figure 5-1. 공기 주입량이 Nostoc HN601 생육시 폐액의 질소 제거에 미치는 영향 (2L 배양, 1차 여과 원액) Figure 5-2. 공기 주입량이 Nostoc HN601 생육시 폐액의 질소 제거에 미치는 영향 (9L 배양, 1차 여과 원액) Figure 5-3. 공기 주입량이 Nostoc HN601 생육시 희석 폐액(2배)의 질소 제거에 미치는 영향 (1.5L 배양, 1차 여과 2배 희석액) Figure 5-4. 공기 주입량이 Nostoc HN601 생육시 희석 폐액(2배)의 질소 제거에 미치는 영향 (7L 배양, 1차 여과 2배 희석액)
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28 W a ter S e al CH 4 WGM Pall Ring Pall Ring E fflu en t Swine W astew ater Tubing Pum p 균 체 이 단 혼 합 형 혐 기 성 소 화 생 물 학 적 N, P 제 거
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37 제 3 절 생물학적 N,P 제거 및 균체 이용방안
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44 UASB Reactor AF Reactor Two-Stage AF Two-Stage SP Two-Stage MO Biogas Biogas 35 C Chamber Wet Gas Meter Wet Gas Meter Biogas Biogas Biogas GSS Effluent Effluent 35 C Chamber Wet Gas Meter Wet Gas Meter Wet Gas Meter # 8 # 8 # 7 # 7 Effluent Effluent Effluent # 6 # 6 # 5 # 5 #4 #8 #4 #8 #4 #8 Pall Ring Pall Ring Sponge Sponge Module Module # 4 # 4 #3 #7 #3 #7 #3 #7 # 3 # 3 #2 #6 #2 #6 #2 #6 # 2 # 2 Influent #1 #5 Sampling Influent #1 #5 Sampling Influent #1 #5 Sampling Port Port Port Influent # 1 Sampling Port Influent # 1 Sampling Port Pump Pump Pump Pump Pump
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47 Wet Gas Meter 유출 Pall Ring Pall Ring 60cm 유입 Tubing Pump 16cm D 19cm 1단소화조 2단소화조
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55 100 Effluent COD Removal Efficiency(%) AF Hy brid-sp Hy brid-pr Time since Operation(days) AF Alkal. Hybrid-SP Alkal. Hybrid-PR Alkal. AF ph Hybrid-SP ph Hybrid-PR ph Effluent Alkalinity (mg/l as CaCO 3) Effluent ph Time since Operation(days) 5
56 Influent Hybrid-SP AF Hybrid-PR Effluent TKN(mg/L) Time since Operation(days) Influent Hybrid-SP AF Hybrid-PR Effluent TP (mg/l) Time since Operation(days)
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58 ASBF-C1 alka ASBF-C2alka ASBF-C1pH ASBF-C2pH Alkalinity(mg/L as CaCO 3) Phase I II III IV Phase V Time since operation(days) ph
59 Influent COD ASBF-C2 Phase I II III IV Phase V COD concentration(mg/l) Time Since Operation(days) 100% Phase I II III IV Phase V 90% 80% COD Removal(%) 70% 60% 50% 40% 30% 20% 10% 0% Time Since Operation(days)
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61 6000 ASBF-C1 ASBF-C VFA conc.(mg/l as acetic acid) time since operation(days) COD Removal Efficiency(%) Organic Loading Rate(kgCOD/m 3 -d) after recover
62 OLRs (Phase I) (Phase II) (Phase III) (Phase IV) (Phase V) (kgcod/ m 3 d) COD Val(%) Gas Produ-ction Remo- Gas Production COD Remo- Val(%) Gas Production COD Remo- Val(%) Gas Produ- Ction COD Remo- Val(%) COD Remo- Val(%) Gas Produ-ction ASBF-C (-) (0.232) (0.203) (0.185) (0.195) *( )은 CH4 yield(m3/kg COD removed) * biogas production(m3/kg COD removed)
63 TKN(mg/L) Phase I II III IV Phase V Time since operation(days) Effluent TP concentration(mg/l) Phase I II III IV Phase V Time since operation(days)
64 16000 Phase I II III IV Phase V Cumulative gas production(l) Time since operation(days) 60 Phase I II III IV Phase V 50 CH 4 함유율(%) time since operation(days)
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67 ASBF-C2 TSS ASBF-C2 VSS TSS/VSS(mg/L) Phase I II III IV Phase V Time since operation(days) 35,000 2kgCOD/ m3.d 4kgCOD/ m3.d VSS concentration(mg/l) 30,000 25,000 20,000 15,000 10,000 5,000 0 E1-10 E1-20 E1-30 E1-40 E2-10 E2-20 E2-30 E2-40 Bioreactor height(cm)
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69 Alkalinity(mg/L as CaCO 3) ASBF-SWl Alkali ASBF-SW2 Alkali ASBF-SW1 ph ASBF-SW2 ph Time since operation(days) ph
70 12000 Influent COD ASBF-C COD concentration(mg/l) Time Since Operation(days)
71 120% 100% COD Removal(%) 80% 60% 40% 20% 0% Time Since Operation(days)
72 OLRs (kgcod/ m 3 d) 2 (Phase I) COD Remo- Val(%) ASBF-SW 96.6 Gas Production (-) 4 (Phase II) COD Remo- Val(%) 0 Gas Production 0 (0) 6 (Phase III) COD Remo- Val(%) 68.1 Gas Produ- Ction (0.251) COD Remo- Val(%) 10 (Phase IV) Gas Produ-ction COD Remo- Val(%) 5 (Phase V) Gas Produ-ction *( )은 CH 4 yield(m 3 /kg COD removed) * biogas production(m 3 /kg COD removed) (0.158) (0.213)
73 6000 ASBF-SW1 ASBF-SW2 VFAs conc.(mg/l as acetic acid) Time since operation(days) 100 after adaptation COD Removal Efficiency(%) Organic Loading Rate(kgCOD/m 3 -d)
74 2000 Effluent TKN concentration (mg/l) Time since operation(day s)
75 Effluent TP concentration (mg/l) Time since operation(days)
76 cumulative gas production(l) Time since operation(days) CH 4 함유율(%) Time since poperation(days)
77 Effluent TSS & VSS concentration(mg/l) ASBF-SW2 TSS ASBF-SW2 VSS Time since operation(days)
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81 Pressure(N2 Gas) Sample Reservoir Retentate Membrane Filter Permeate Internal Recirculation Pump Holding Pad TOC(%) 100% 90% 80% 70% 60% 50% 40% 30% 20% 10% 0% >XM300 YM100-XM300 XM50-YM100 YM10-XM50 YM3-YM10 YM1-YM3 <YM1 Influent TOC : 523ppm Effluent TOC : 91ppm Feed Effluent Two-Stage ASBF
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83 MgCl 2 H 3 PO 4 PH Control Heater Biogas Influent MF NF Effluent From MF Concentrate To Preheater To Composter MAP PPT Pre-Treatment Pre-Heater Two Stage ASBF Membrane Post-Treatment
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107 motor WGM Peristaltic pump 38 Waterbath
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109 1st batch, steady-state (after 6 batches) COD(mg/L), Gas production(l) COD concentration (mg/l) MCR cumulative gas production(l) Incubation Time(days)
110 1st batch, after 3 batches, after 6 batches; COD(mg/L), Gas production(l) COD concentration (mg/l) MCR cumulative gas production(l) Incubation Time(day s )
111 Control, 1,500 mg/l, 2,500 mg/l COD(mg/L), ---- Gas production(l) COD concentration (mg/l) MCR Incubation Time(days) cumulative gas production(l) COD concentration(mg/l) MCR Incubation Time (days) cumulative gas production(l)
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125 DAT Glucose Sucrose 0.12% 0.48% 0.12% 0.48% % DAT Glucose Sucrose 0.12% 0.48% 0.12% 0.48% %
126 (NH 4 ) 2 SO 4 NaNO 3 DAT 1mM-N 4mM-N 1mM-N 4mM-N % (NH 4) 2SO 4 NaNO 3 DAT 1mM-N 4mM-N 1mM-N 4mM-N %
127 Isolate Nitrogen Phosphorus g-n/gdw mg-p/gdw Nostoc HN Anabaena HA Anabaena HA Anabaena HA Nostoc HN Anabaena HA Cylindrosprum Sytonema Calothrix HC Calothrix HC Calothrix HC CH Anabaena HA Calothrix HC Calothrix HC Nostoc HN RF Hepalosiphon
128 Isolate Days after Growth Chlorophyll a (mg/l) Anabaena HA HA HA HA HA Nostoc HN HN HN HN HN
129 Isolate Days after Growth Phosphate (mg P/L) Anabaena HA HA HA HA HA Nostoc HN HN HN HN HN
130 Isolate Days after Growth Nitrate (mg N/L) Anabaena HA HA HA HA HA Nostoc HN HN HN HN HN
131 D A T ph % of ph D A T ph % of ph D A T ph % of ph
132 L(폐수)(0.5L/min) 2L(폐수)(0.3L/min) NH4-N(mg / L) Days
133 L(폐수)(5.0L/min) 9L(폐수)(2.5L/min) NH4-N(mg / L) Days
134 L(폐수)(0.5L/min) 1.5L(폐수)(0.3L/min) NH4-N(mg / L) Days
135 L(폐수)(5.0L/min) 7L(폐수)(2.5L/min) NH4-N(mg / L) Days
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Development of culture technic for practical cultivation under structure in Gastrodia elate Blume
Development of culture technic for practical cultivation under structure in Gastrodia elate Blume 1996. : 1. 8 2. 1 1998. 12. : : ( ) : . 1998. 12 : : : : : : : : : : - 1 - .. 1.... 2.. 3.... 1..,,.,,
PJTROHMPCJPS.hwp
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