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1 농약과학회지 (Korean J. Pestic. Sci.) Vol. 20, No. 1, pp (2016) Open Access Online ISSN Print ISSN ORIGINAL ARTICLES / CONTROL 칠레이리응애의살충제에대한감수성 김무성 김상수 * 순천대학교식물의학과 Susceptibility of the Predatory Mite, Phytoseiulus persimilis (Acari: Phytoseiidae) to Several Insecticides Moo Sung Kim and Sang Soo Kim* Dept. of Plant Medicine, Sunchon National University, Sunchon 57922, Korea (Received on February 21, Revised on March 9, Accepted on March 10, 2016) Abstract Susceptibility of the predatory mite, Phytoseiulus persimilis to 8 insecticides was evaluated. In treatments with insecticides tested, 78-92% of adult female predators survived after 168 hrs after treatment. Adult female predators treated with insecticides tested produced % as many eggs as did control females and eclosion of eggs deposited by treated predators was not affected. Moreover, hatch percentage of P. persimilis eggs and survival of nymphs were not seriously affected by exposure to the insecticides tested. Immatures of P. persimilis survived on the leaf disc treated with insecticides tested and % of immature predators reached adulthood. Based on the results, insecticides tested are expected to be promising candidates for use in integrated pest management program with P. persimilis. Key words Phytoseiulus persimilis, insecticides, susceptibility, integrated pest management 서 론 칠레이리응애 (Phytoseiulus persimilis) 는다양한작물에발생하여가해하는점박이응애 (Tetranychus urticae) 에대한효과적인포식성천적으로여러나라에서보고되어왔으며, 특히온실재배작물에서는광범위하게실용화되고있는종이다 (Jarosik and Pliva, 1990; Drukker et al., 1997; Yoo and Kim, 2000; Cote et al., 2004; Seo et al., 2004). 이와같은칠레이리응애는우리나라에도도입되어장미, 딸기, 가지와신선초등에서이천적을이용한점박이응애나차응애 (T. kanzawai) 의밀도억제효과와활용방안에대해보고된바있다 (Kim et al., 1999; Lee et al., 2002; Ahn et al., 2004; Kim et al., 2006b; Moon et al., 2006). 그러나이와같은유력한천적이도입되어활용되어도일반적으로재배작물에발생하는다른해충류의방제를위해살충제를사용하는경우가있을뿐만아니라식식성응애류의발생밀도가 *Corresponding author kimss@sunchon.ac.kr 높아천적에의한방제효과가저조할경우에살응애제의불가피한사용이있을수있으며 (Ahn et al., 2004; Choi et al., 2007), 여러재배작물에서약제사용을완전히배제한상태에서이리응애만으로는식식성응애류의개체군밀도를경제적피해수준이하로유지하기어렵다고보고된바있다 (Lee, 1990; Yoo and Kim, 2000; Sato et al., 2007). 더구나생물적방제인자로서이용되고있는포식성및기생성천적들은일반적으로약제에매우민감하다는문제점이있다 (Kim et al., 2006a; Choi et al., 2007). 이러한배경에서국내외에서점박이응애나차응애의방제체계에칠레이리응애를투입하고, 천적에저독성인살응애제, 살충제및살균제를탐색이용하여천적과해충의밀도를적정수준으로조정함으로서보다장기적인방제효과를유지하려는연구가이루어져왔다 (Lee et al., 2002; Cote et al., 2004; Ahn et al., 2004; Seo et al., 2004; Bostanian and Akalach, 2006). 또한 Trumble and Morse (1993) 는딸기에서점박이응애의방제를위한약제사용, 천적의이용및약제와천적의동시이용의경제성을비교한결과, 칠레이리응애와 abamectin의조합이용에서최대의이윤을얻었다고보고한바있다. 66

2 칠레이리응애의살충제에대한감수성 67 이에본실험은여러작물에발생하는나방류, 진딧물류, 총채벌레류또는가루이류등에대한방제약제로등록된몇가지살충제들을대상으로칠레이리응애의발육단계별생존율과산란수및부화율등에미치는영향을실험하여, 해충종합관리체계에서이들살충제와칠레이리응애의동시활용가능성을검토하고자수행하였다. 재료및방법 칠레이리응애의사육본실험에사용한칠레이리응애는 Biobest (Belgium) 에서분양받아 25 ± 2 o C의사육실에서강낭콩 (Phaseolus vulgaris var. humilis Alefeld) 잎에점박이응애를먹이로공급하면서누대사육하였다. 점박이응애는순천의배재배지에서채집하고강낭콩에사육 증식하여확보하였다. 실험도구로물을채운플라스틱밀폐용기 (14 5 cm) 의덮개중앙에 1cm의구멍을뚫은다음, 하부중앙에같은크기로구멍을뚫어탈지면을깐플라스틱페트리디쉬 ( 직경 9cm) 를그위에놓고탈지면으로서로연결해계속하여수분이공급될수있도록하였다. 이페트리디쉬에칠레이리응애를접종할강낭콩잎절편 ( 직경 3cm) 의뒷면이위를향하도록놓은다음잎절편주위에는물에적신탈지면을배치해칠레이리응애의이탈을방지하였다. 약제처리후에는 25 ± 1 o C (16L:8D, RH 50-60%) 의조건에보관하면서수행하였다. 약제처리실험에사용한살충제는 chlorantraniliprole 5% 입상수화제. flonicamid 10% 입상수화제, flubendiamide 20% 액상수화제, indoxacarb 5% 액상수화제, metaflumizone 20% 액상수화제, pymetrozine 25% 수화제, spinetoram 5% 입상수화제와 thiacloprid 10% 액상수화제등이었으며, 시판제품을사용하였다. 실험약제를추천농도로희석하고전술한페트리디쉬내에배치한칠레이리응애의성충, 약충또는난이접종되어있는잎절편을대상으로 25 cm 정도의거리에서 hand sprayer (Komax co., Korea) 를이용하여잎절편이충분히적실정도로살포하는엽면살포방법 (Hoy et al., 1988) 으로실시한후 1시간동안음건하였다. 사망여부의판단으로난은부화여부를, 약충과성충의경우는가는붓으로충체를접촉하여반응이없거나몸길이정도를이동하지못하는개체를죽은것으로간주하였다. 칠레이리응애의각발육단계에대한약제들의영향칠레이리응애암컷성충의생존율과산란수에미치는약제들의영향을실험하기위해각약제별 50개체 ( 반복당 10개체 ) 로수행하였다. 전술한페트리디쉬내의물에적셔진탈지면위에뒷면이위를향하도록놓은강남콩잎절편 ( 직경 3cm) 에유사한연령의암컷성충을미세한붓으로잎절편으로옮긴후, 각약제를살포한후음건하였다. 칠레이리응애의먹이로는점박이응애를제공하였고매일보충하여충분한먹이조건을유지하였다. 암컷성충들의생존율과잎절편의전체산란수는약제처리 1, 3, 5, 7일후에현미경하에서조사하였으며, 산란된난들은별개의무처리잎절편에옮기고부화율을조사하였다. 암컷성충에대한실험결과는국제생물적방제기구 (IOBC) 에서설정한약제의유용생물에대한부작용등급의기준 (Hassan, 1994) 에따라치사율이 <30% 는영향이없음, 30-79% 는약간영향이있음, 80-99% 는상당한영향이있음, >99% 는악영향이있음으로분류하였다. 칠레이리응애의난에대한약제들의영향을실험하기위하여각각의강낭콩잎절편 ( 직경 4cm) 에칠레이리응애암컷성충 7-8개체를접종하여 1일동안산란시킨후성충을제거하고필요한난수로조정하였다. 각약제별 50개체 ( 반복당 10개체 ) 로실험하였으며, 약제의희석액을살포한후음건하였다. 이후난의부화여부를매일조사하였다. 칠레이리응애의약충에대한약제들의영향을실험하기위하여각각의강낭콩잎절편 ( 직경 3cm) 에칠레이리응애약충 10개체를미세한붓으로옮긴후, 각약제의희석액을살포한후음건하였다. 이후점박이응애를제공하면서약충의생존여부를무처리의개체들이성충태에도달할때까지매일조사하였으며, 각약제별 50개체 ( 반복당 10개체 ) 로실험하였다. 실험약제를처리한강낭콩잎절편에서칠레이리응애의발육태별생존에대한영향을각약제별 50개체 ( 반복당 10 개체 ) 로실시하였다. 페트리디쉬내의잎절편 ( 직경 3cm) 에각약제의희석액을살포하여음건하고, 산란 1일이내의난을옮긴후부화와유 약충의생존여부를매일조사하였으며, 모든유 약충이성충이되면시험을종료하였다. 먹이로는칠레이리응애의난이부화하기시작할때부터점박이응애를계속하여충분히공급하였다. 자료분석각경우의실험에서생존율, 부화율과산란수에대한결과는분산분석 (ANOVA) 과 Tukey test (SAS Institute, 2002) 로비교하였으며, 생존율과부화율에대한성적은 arcsine 값으로변환한후분석에이용하였다. 결과및고찰 칠레이리응애의암컷성충에대한약제들의영향을실험한결과는 Table 1과같다. 칠레이리응애의생존율은실험약제들의처리 24시간후에 % 로매우높았으나, 모든약제처리에서일수경과에따라생존율이감소하는경향이었다.

3 68 김무성 김상수 Table 1. Survival of P. persimilis female adults on bean leaf discs treated with insecticides Insecticides treated Treatment % Survival (Mean ± SE) after a rate 24 h 72 h 120 h 168 h Chlorantraniliprole 0.5 g/l ± 0 a ± 0 a 92.0 ± 2.0 a 88.0 ± 2.0 ab Flonicamid 0.5 g/l ± 0 a 94.0 ± 4.0 a 90.0 ± 4.5 a 90.0 ± 4.5 ab Flubendiamide 0.5 ml/l ± 0 a 94.0 ± 2.5 a 88.0 ± 2.0 a 88.0 ± 2.0 ab Indoxacarb 1 ml/l ± 0 a 96.0 ± 2.5 a 96.0 ± 2.5 a 92.0 ± 3.7 ab Metaflumizone 0.67 ml/l 96.0 ± 2.5 a 92.0 ± 2.0 a 86.0 ± 4.0 a 84.0 ± 2.5 ab Pymetrozine 0.33 g/l 96.0 ± 2.5 a 90.0 ± 3.2 a 88.0 ± 2.0 a 88.0 ± 2.0 ab Spinetoram 0.5 g/l ± 0 a 96.0 ± 2.5 a 92.0 ± 3.7 a 86.0 ± 2.5 ab Thiacloprid 0.5 ml/l 98.0 ± 2.0 a 88.0 ± 4.9 a 86.0 ± 4.0 a 78.0 ± 3.7 b Control ± ± 0 a 96.0 ± 2.5 a 94.0 ± 2.5 a a Means in the same column followed by the same letter are not significantly different (p=0.05, Tukey test). Mortality was transformed to arcsine value before ANOVA. Means of untransformed data are reported. Table 2. Reproduction of P. persimilis female adults on bean leaf discs treated with insecticides and eclosion of the eggs Insecticides treated Number of eggs per leaf disc (Mean ± SE) a % Eclosion (Mean ± SEM) a Chlorantraniliprole ± 4.5 cd 99.9 ± 0.1 a Flonicamid ± 8.3 bcd 99.8 ± 0.2 a Flubendiamide ± 6.2 ab 99.8 ± 0.1 a Indoxacarb ± 7.2 ab 99.6 ± 0.2 a Metaflumizone ± 5.9 d 99.9 ± 0.1 a Pymetrozine ± 6.5 d ± 0 a Spinetoram ± 7.0 abc 99.5 ± 0.3 a Thiacloprid ± 11.9 cd 99.7 ± 0.2 a Control ± 4.3 a ± 0 a a Means in the same column followed by the same letter are not significantly different (p=0.05, Tukey test). Table 3. Effects of insecticides on eggs and nymphs of P. persimilis Insecticides treated % Hatchability (Mean ± SE) a % Survival of nymphs (Mean ± SE) a Chlorantraniliprole ± 0 a 92.0 ± 2.0 ab Flonicamid ± 0 a 80.0 ± 6.1 b Flubendiamide ± 0 a 80.0 ± 4.5 b Indoxacarb ± 0 a 88.0 ± 3.7 b Metaflumizone ± 0 a 78.0 ± 3.7 b Pymetrozine ± 0 a 88.0 ± 5.8 ab Spinetoram ± 0 a 82.0 ± 3.7 b Thiacloprid ± 0 a 76.0 ± 6.8 b Control ± 0 a ± 0 a a Means in the same column followed by the same letter are not significantly different (p=0.05, Tukey test). 처리 168시간후의칠레이리응애생존율은 78-90% 로 thiacloprid를제외한다른약제들의처리는무처리의생존율과통계적유의차가없었다. 이와같은결과를국제생물적방제기구 (IOBC) 에서설정한기준 (Hassan, 1994) 에따르면, 모든약제처리에서 30% 이내의치사율로영향이없음 (1등급) 으로나타났다. 칠레이리응애암컷성충에대해실험약제들을처리하였을때산란수와부화율을조사한결과는 Table 2와같다. 칠레이리응애의산란수는각약제처리에서모두무처리와통계적유의차는있으나, chlorantraniliprole, flonicamid, flubendiamide, indoxacarb, spinetoram과 thiacloprid 등의처리는무처리와비교하여 % 의산란수를보였으며, metaflumizone과 pymetrozine의경우는다른약제처리에비해다소적은 62.6과 65% 의산란수를나타내었다. 또한산란된난들은모든약제처리에서 % 부화하였다. 이와같은생존율, 산란수및그부화율로보아, 이들실험약제의사용후칠레이리응애암컷성충의밀도유지에 직접적인영향을주지않을것으로판단된다. 칠레이리응애의난과약충에대해실험약제들을처리하여부화율과생존율에미치는영향을조사한결과는 Table 3과같다. 칠레이리응애의난은모든처리에서 100% 부화하여, 실험약제들은칠레이리응애난에대한살란효과는없는것으로나타났다. 칠레이리응애의약충은모든약제처리에서 76-92% 의생존율로암컷성충에대한실험결과와같이 30% 이내의낮은치사율을보였다. 이와같은결과로보아실험약제들은칠레이리응애의약충의생존율에큰영향이없는것으로생각된다. 실험약제들을처리한잎절편에서칠레이리응애각발육태의생존율을조사한결과는 Table 4와같다. 칠레이리응애는 flonicad, flubendiamide와 thiacloprid 처리에서약충기에각 2% 의치사율로, 모든처리에서 % 의성충우화율을나타내었다. 따라서각발육태에대해잔류되어있는실험약제들의영향은거의없는것으로나타났다.

4 칠레이리응애의살충제에대한감수성 69 Table 4. Effects of insecticides on immature stages of P. persimilis on treated bean leaf discs Insecticides treated % Motality (Mean ± SE) at a % Survival Egg stage Larval stage Nymphal stage to adulthood a Chlorantraniliprole 0.0 ± ± ± ± 0 a Flonicamid 0.0 ± ± ± ± 2.0 a Flubendiamide 0.0 ± ± ± ± 2.0 a Indoxacarb 0.0 ± ± ± ± 0 a Metaflumizone 0.0 ± ± ± ± 0 a Pymetrozine 0.0 ± ± ± ± 0 a Spinetoram 0.0 ± ± ± ± 0 a Thiacloprid 0.0 ± ± ± ± 2.0 a Control 0.0 ± ± ± ± 0 a a Means in the same column followed by the same letter are not significantly different (p=0.05, Tukey test). 실험약제들의포식성응애류에대한영향을연구한결과들을살펴보면, chlorantraniliprole과 metaflumizone은지중해이리응애 (Amblyseius swirskii) 의성충에대한저독성이보고되었으며 (Gradish et al., 2011), Lefebvre et al. (2012) 도 diamide제인 chlorantraniliprole과 flubendiamide는팔라시스이리응애 (Neoseiulus fallacis) 의난, 유충, 성충에저독성을나타냈다고하였다. Indoxacarb는 Bostanian and Akalach (2006) 에의해칠레이리응애와팔라시스이리응애의성충과난에안전한약제라고보고된바있다. Pymetrozine은칠레이리응애의성충과난에대한저독성이보고되었으며 (Duso et al., 2008), 유사한약효발현양식 (feeding blocker) 을나타내는 flonicamid는지중해이리응애에영향이없는것으로보고되었다 (Colomer et al., 2011). 한편 Cuthbertson et al. (2012) 은지중해이리응애, 오이이리응애 (N. cucumeris) 와 Typhlodromips montdorensis, Iphiseius degenerans 등의포식성응애류에대해 thiacloprid의영향을실험한결과앞서의 3종포식성응애류를제외한 I. degenerans에만높은치사율을보여동일한약제가이리응애의종에따라상반된영향을나타냈다고보고하였다. 또한 Bostanian et al. (2009, 2010) 은 thiacloprid가서양이리응애의성충및팔라시스이리응애의성충과난에대한치사율이매우낮으나, 동일한 neonicotinoid제인 imidacloprid는이들이리응애류의성충에 100% 에달하는치사율을나타냈다고하여, 국내에서여러종의 neonicotinoid제가사용되고있어약종의범위를확대하여실험할필요가있는것으로생각된다. 이와같이 chlorantraniliprole. flonicamid, flubendiamide, indoxacarb, metaflumizone, pymetrozine과 thiacloprid에대해보고된내용들은본실험의결과와같은경향이었다. Spinetoram의경우는본실험에서칠레이리응애에저독성인것으로나타났다. 그러나 Lefebvre et al. (2012) 은이약제가팔라시스이리응애의난에는저독성이지만유충과성충에는 90% 이상의치사율을나타낸다고보고하여, 본실험에서의결과와 차이가있었다. 이는실험대상포식성응애종의차이에기인한결과로생각되며, Ahn et al. (2004) 도천적응애의종류에따라살비제독성에차이가있을수있으므로약제와함께처리할때에는충분한검토후에사용해야한다고언급한바있다. 위와같은실험결과들을종합해보면실험약제들은모두칠레이리응애의암컷성충과난및약충에대한직접처리에서저독성을나타내었으며약제들이처리된잎절편에서칠레이리응애의발육태생존율에큰영향이없어, 해충종합관리체계에서칠레이리응애와함께활용할수있을것으로기대된다. 또한재배작물에따라점박이응애나차응애이외에이들약제의적용해충으로등록되어있는진딧물류, 총채벌레류, 가루이류또는나방류와같은해충들이동시에발생될경우에는, 본실험의칠레이리응애에저독성을보인약제들이이들해충류의방제약제이므로칠레이리응애와약제를같이사용하여식식성응애류와해충들을동시에방제할수있을것으로기대된다. Literature Cited Ahn, K. S., S. Y. Lee, K. Y. Lee, Y. S. Lee and G. H. Kim (2004) Selective toxicity of pesticides to the predatory mite, Phytoseiulus persimilis and control effects of the twospotted spider mite, Tetranychus urticae by predatory mite and pesticide mixture. Korean J. Appl. Entomol. 43: Bostanian, N. J. and M. Akalach (2006) The effect of indoxacarb and five other insecticides on Phytoseiulus persimilis (Acari: Phytoseiidae), Amblyseius fallacis (Acari: Phytoseiidae) and nymphs of Orius insidiosus (Hemiptera: Anthocoridae). Pest Manag. Sci. 62: Bostanian, N. J., J. M. Hardman, H. A. Thistlewood M. C. Laurin and G. Racette (2009) Effects of seven new orchard pesticides on Galendromus occidentalis in laboratory studies. Pest Manag. Sci. 65:

5 70 김무성 김상수 Bostanian, N. J., J. M. Hardman, H. A. Thistlewood and G. Racette (2010) Effects of six selected orchard insecticides on Neoseiulus fallacis (Acari: Phytoseiidae) in the laboratory. Pest Manag. Sci. 66: Choi, B. R., H. M. Park, J. H. Kim and S. W. Lee (2007) Evaluation of low toxic and residual toxicity of pesticides registered on sweet pepper greenhouse to Orius strigicollis. Korean J. Appl. Entomol. 46: Colomer, I., P. Aguado, P. Medina, R. M. Heredia, A. Fereres, J. E. Belda and E. Vinuela (2011) Field trial measuring the compatibility of methoxyfenozide and flonicamid with Orius laevigatus Fieber (Hemiptera: Anthocoridae) and Amblyseius swirskii (Athias-Henriot) (Acari: Phytoseiidae) in a commercial pepper. Pest Manag. Sci. 67: Cote, K. W., P. B. Schultz and E. E. Lewis (2004) Using acaricides in combination with Phytoseiulus persimilis Athias-Henriot to suppress Tetranychus urticae Koch populations. J. Entomol. Sci. 39: Cuthbertson, A. G. S., J. J. Mathers, P. Croft, N. Nattriss, L. F. Blackburn, W. Luo. P. Northing, T. Murai, R. J. Jacobson and K. F. A. Walters (2012) Prey consumption rates and compatibility with pesticides of four predatory mites from the family Phytoseiidae attacking Thrips palmi Karny (Thysanoptera: Thripidae). Pest Manag. Sci. 68: Drukker, B., A. Janssen, W. Ravensberg and M. W. Sabelis (1997) Improved control capacity of the mite predator Phytoseiulus persimilis (Acari: Phytoseiidae) on tomato. Exp. Appl. Acarol. 21: Duso, C., V. Malagnini, A. Pozzebon, M. Castagnoli, M. Liguori and S. Simini (2008) Comparative toxicity of botanical and reduced-risk insecticides to Mediterranean populations of Tetranychus urticae and Phytoseiulus persimilis (Acari: Tetranychidae, Phytoseiidae). Biol. Control 47: Gradish, A. E., C. D. Scott-Dupree, L. Shipp, C. Ron Harris and G. Ferguson (2011) Effect of reduced risk pesticides on greenhouse vegetable arthropod biological control agents. Pest Manag. Sci. 67: Hassan, S. A. (1994) Activities of the IOBC/WPRS working group "Pesticides and Beneficial Organisms". In: Pesticides and Beneficial Organisms. (ed., Vogt H.). IOBC/WPRS Bulletin 17:1-5. Hoy, M. A., J. Conley and W. Robinson (1988) Cyhexatin and fenbutatin-oxide resistance in pacific spider mite (Acari: Tetranychidae): stability and mode of inheritance. J. Econ. Entomol. 81: Jarosik, V. and J. Pliva (1990) Efficient control of twospotted spider mite (Tetranychus urticae Koch) by Phytoseiulus persimilis A.-H. on glasshouse peppers. J. Appl. Ent. 110: Kim, D. H., S. S. Kim, K. S. Kim and J. W. Hyun (2006a) Comparative toxicity of some pesticides to the predatory mite, Neoseiulus fallacis Garman (Acari: Phytoseiidae). Korean J. Appl. Entomol. 45: Kim, D. I., S. G. Kim, G. H. Shin, J. B. Seo, K. J. Choi, K. H. Lim and S. S. Kim (2006b) Biological control of twospotted spider mite (Tetranychus urticae) by the predatory mite (Phytoseiulus persimilis) in sustainable strawberry fields. Korean J. Org. Agric. 14: Kim, Y. H., J. H. Kim and M. W. Han (1999) A preliminary study on the biological control of Teranychus kanzawai Kishida in Angelica utilis Makino by Phytoseiulus persimilis Athias-Henriot (Acarina: Tetranychidae, Phytoseiidae). Korean J. Appl. Entomol. 38: Lee, S. G., S. A. Hilton, A. B. Broadbent and J. H. Kim (2002) Insecticide resistance in phytoseiid predatory mites, Phytoseiulus persimilis and Amblyseius cucumeris (Acarina: Phytoseiidae). J. Asia-Pacific Entomol. 5: Lee, S. W. (1990) Studies on the pest status and integrated mite management in apple orchards. Ph.D. thesis, Seoul Na;l Univ. 87pp. Lefebvre, M., N. J. Bostanian, Y. Mauffette, G. Racette, H. A. Thistlewood and J. A. Hardman (2012) Laboratory-based toxicological assessments of new insecticides on mortality and fecundity of Neoseiulus fallacis (Acari: Phytoseiidae). J. Econ. Entomol. 105: Moon, H. C., J. R. Lim, J. Kim, J. Ryu, B. R. Ko, D. H. Kim and C. Y. Hwang (2006) Biological control of Tetranychus urticae by Phytoseiulus persimilis in eggplant greenhouse houses. Korean J. Appl. Entomol. 45: SAS Institute (2002) User's guide, version SAS Institute, Cary, NC, USA. Sato, M. E., M. Z. da Silva, M. F. de Souza Filho, A. L. Matioli, and A. Raga (2007) Management of Tetranychus urticae (Acari: Tetranychidae) in strawberry fields with Neoseiulus californicus (Acari: Phytoseiidae) and acaricides. Exp. Appl. Acarol. 42: Seo, S. G., S. S. Kim, J. D. Park, S. G. Kim and D. I. Kim (2004) Selective toxicity of spirodiclofen and fluacrypyrim + tetradifon to the predatory mite, Phytoseiulus persimilis (Acarina: Phytoseiidae) and the tea red spider mite, Tetranychus kanzawai (Acarina: Tetranychidae). Korean J. Pestic.Sci. 8: Trumble, J. T. and J. P. Morse (1993) Economics of integrating the predacious mite Phytoseiulus persimilis (Acari: Phytoseiidae) with pesticides in strawberries. J. Econ. Entomol. 86: Yoo, S. S. and S. S. Kim (2000) Comparative toxicity of some pesticides to the predatory mite, Phytoseiulus persimilis (Acarina: Phytoseiidae) and the two spotted spider mite, Tetranychus urticae (Acarina: Tetranychidae). Korean J. Entomol. 30:

6 칠레이리응애의살충제에대한감수성 71 칠레이리응애의살충제에대한감수성 김무성 김상수 * 순천대학교식물의학과 요약포식성천적칠레이리응애에대하여 8 종살충제들의감수성을검정하였다. 칠레이리응애암컷성충은실험약제들의처리 168 시간후에 78-92% 의개체들이생존하였으며, 무처리암컷성충에비해 % 의산란수를보였으며, 산란된난들의부화에영향이없었다. 실험약제들은칠레이리응애난의부화와약충의생존율에큰영향이없었다. 칠레이리응애의발육태들은실험약제들이처리된잎절편에서생존율에큰영향을받지않아, % 의개체들이성충태에도달하였다. 이상의결과로보아, 실험약제들은해충종합관리체계에서칠레이리응애와함께활용할수있을것으로생각된다. 색인어 칠레이리응애, 살충제, 감수성, 해충종합관리

DBPIA-NURIMEDIA

DBPIA-NURIMEDIA 농약과학회지 (Korean J. Pestic. Sci.) Vol. 19, No. 4, pp. 418-423 (2015) Open Access http://dx.doi.org/10.7585/kjps.2015.19.4.418 Online ISSN 2287-2051 Print ISSN 1226-6183 ORIGINAL ARTICLES / CONTROL 사막이리응애의살응애제에대한감수성