sugar cane
계분 (%)
a ATCC : American type Culture Collection, b IFO : Institute for fermentation, osaka, c MPNU : Mycological lab. Pusan National University d Mycelial growth was measured after 7days of incubation, 30 (Quericus aliena sawdust 70% + rice bran 30%)
70% + Rice bran 30% 70%+Rice bran 30% 70%+Rice bran 30% a Mycelial growth was measured after 7 days of incubation, 30 100 80 60 40 20 0 90 84 67696954 6468 60 697376 705556 67 69 6767 69 7367 66 59 57 55 57 44 47 36 16 Rrice bran 10% Gluten 10% Peptone 2% Yeast extract 2% Scallop extract 2% Beef extract 2% Chicken extract 2% Silk worm 2% Shrimp powder 2% Silk worm pupae pd.2% Dog food 10% Wheat flour 10% Corn flou r 10% Fresh soybean pd.10% IS P 2% Adlay powder 10% Potato powder 10% Sea lettuce powder 2% Molasses 2% Malt powder 10% Sikhae waste 10% CMM * Corn cobs 10% coconut palm 10% PS 5%+ Cs 5% Cotton seed 10% Alfalfa hay 10% CaCl2 0.2% CMC 10% Carrageenan 10% Alginic acid 10%
Contamination percentage (%) 50 45 40 35 30 25 20 15 10 5 0 Rice bran 14 10% Gluten 18 10% Peptone 8 2% Silk worm 12 2% Shrimp 36 powder 2% Beef 12 extract 2% 38 ISP 2% Sea lettuce 28 powder 2% Malt 34 32 powder CMM 100% peanut 44 hulls Silk worm 24 pupae 1 2 3 4 5 6 7 8 9 1 0 1 1 1 2 Fig. 3. Test of contamination possibility for incubation of P.
Contamination percentage (%) 70 60 50 40 30 20 10 0 1h r 3h r 5h r 7h r 10h r Fig. 5. Test of contamination possibility on Condensed Mixed Medium(CMM)
Beef 1,000g + Tap water 3,500ml Enzyme hydrolysis (50± 5, 120rpm, 3hr) Filteration(40mesh) Beef extract 3,000ml (7.1brix, dry weight 213g)
40 50 60 90 120 150 day
Sodium phosphate, Dibasic 6 10 5 8 A.blazei(cm) 4 3 2 6 4 D.Microsporus(cm) 1 2 0 Normal 0.1% 0.2% 0.3% 0.4% 0.5% 0.6% 0.7% 0.8% 0.9% 1% A.blazei D.Microsporus Na 2 HPO 4 concentration (%) 0
Potassium phosphate, Dibasic + Sodium phosphate, Dibasic 6 10 5 8 A.blazei(cm) 4 3 2 6 4 D.Microsporus(cm) 1 2 0 Normal ⅰ ⅱ ⅲ ⅳ ⅴ ⅵ ⅶ mix number 0 ⅰ : Potassium phosphate, Dibasic 0.05% + Sodium phosphate, Dibasic 0.05% ⅱ: Potassium phosphate, Dibasic 0.1% + Sodium phosphate, Dibasic 0.1% ⅲ: Potassium phosphate, Dibasic 0.2% + Sodium phosphate, Dibasic 0.2% ⅳ: Potassium phosphate, Dibasic 0.3% + Sodium phosphate, Dibasic 0.2% ⅴ: Potassium phosphate, Dibasic 0.4% + Sodium phosphate, Dibasic 0.4% ⅵ: Potassium phosphate, Dibasic 0.5% + Sodium phosphate, Dibasic 0.3% ⅶ: Potassium phosphate, Dibasic 0.6% + Sodium phosphate, Dibasic 0.2% D.Microsporus
고고분고고 (%) 16 14 12 10 8 6 4 2 0 자자자인인인인균균균 1:25 1:50 1:100 1:200 버버 (g): 버(ml)
5 5 Yield of polysaccharides(%) 4 3 2 1 자자자인인인인균균균 Yield of polysaccharides(%) 4 3 2 1 자자자인인인인균균균 0 2 3 4 5 Extraction Time (h) 0 60 80 100 Extraction termperature
60 50 * 40 * NO (µm) 30 20 10 0 0 10 50 100 PG (µg/ml) 60 50 40 - PB + PB * * * NO (µm) 30 20 10 0 Control PG LPS
200 180 Cell viability (% of control) 160 140 120 100 80 60 40 20 0 0 10 50 100 LPS PG (µg/ml) Cytotoxicity to YAC-1 (% of control) 300 250 200 150 100 50 0 - L-NMMA + L-NMMA * Control PG LPS *
Medium CD80 CD86 MHC I MHC II 495 1182 197 882 PG 1278 1860 233 1394 LPS 1708 1731 338 1913 Medium CD80 CD86 MHC I MHC II 523 1018 178 956 PG 1284 1901 197 1269 LPS 544 1048 186 790
IL-12 p40/p70-pe Medium PB 2.4% 5.5% 18.2% 14.3% + PG 26.7% 5.0% + LPS CD11c-FITC 200 150 IL-12 p70 (pg/ml) 100 50 0 Bioactive IL-12 production * - PB * + PB * - PG LPS Normal DC PG DC LPS DC
4 / Medium 37 / Medium 6.7% 42.6% UN CD11c-PE 7.6% 22.4% PG 6.3% 28.2% LPS Dextran-FITC
Medium PG Inguinal lymph nodes Medium Brachial lymph nodes PG Cervical lymph nodes LPS Spleen
18.2% PG CD80 CD86 MHC I MHC II PG + G 624/1278 887/1278 1365/1860 1357/1860 206/233 189/233 867/1394 1007/1394 IL-12 p40/p70-pe 5.6% PG + G PG + S 4.8% PG + S CD11c-FITC 120 kda- Medium Genistein Genistein + PG PG PKC activity (Percentage of basal 250 200 150 100 50 0-50 PG PG+H-89 PG+S PMA 75 kda- 50 kda- 37 kda- 25 kda-
UN PG LPS (20 min) p-erk1/2 ERK1/2 p-p38 p38 p65
PG + PD PG + SB PG + PDTC CD86 MHC II IL-12p40/p70-PE PG PG + PD PG + SB PG + PDTC 28.8% 8.7% 11.3% 6.4% CD11c-FITC
Isotype (A) Agaricus blazei (B) Cordyceps millitaris CD80 CD86 MHC I MHC II CD80 CD86 MHC I MHC II Medium PS LPS
IL-12p70 (pg/ml) (A) Agaricus blazei 160 140 120 100 80 60 40 20 * * IL-12p70 (pg/ml) (B) Cordyceps millitaris 120 100 80 60 40 20 * * 0 Medium LPS PS 0 Medium LPS PS
(A) Agaricus blazei Medium PS 4.1 4.0 (B) Cordyceps millitaris LPS Medium PS 4.8 3.1 LPS 3.7 4.3 4 70.5 24.0 38.9 69.0 40.9 20.5 37
(A) Agaricus blazei (B) Cordyceps millitaris IL-2 (pg/ml) 1400 1200 1000 800 600 * * IL-2 (pg/ml) 2500 2000 1500 1000 * * 400 200 500 0 Medium LPS PS 0 Medium LPS PS
beta-glucan contents(%) 16 14 12 10 8 6 4 2 0 Fig. 73.Beta-glucan contents of Agaricus blazei Murill 10.8 Rice straw compost media 14.7 The root of arrowroot
Fig 74. Cordycepin contents of various length of fruitng body in same strain 600 542.77 cordycepin contents (ppm) 500 400 300 200 386.58 360.78 100 0 2cm 5cm 10cm Length of fruiting body Fig. 75. Cordycepin contents in various strains Cordycepin contents(mg/100 fruiting body) 80 70 60 50 40 30 20 10 0 71.7 머쉬텍균주 1.24 건세농장균주 14 16 일조산업균주 청원농산균주