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1 Korean Journal of Obstetrics and Gynecology Vol. 51 No. 12 December 2008 조사보고서한국보조생식술의현황 : 2005년 대한산부인과학회 보조생식술소위원회 Current status of assisted reproductive technology in Korea, 2005 접수일 : 교신저자 : 최영민 ymchoi@snu.ac.kr 제 1 장서론 1986 년국내에서체외수정시술에의한첫시험관아기가탄생한이래불임환자치료를위한보조생식술은날로그시술기관과시행수가증가되고있을뿐만아니라, 치료성적도괄목할만큼개선되는등불임증진단과치료에많은발전이있어왔다. 불임을치료하기위해보조생식술을시행하고있는의료기관으로부터의자료는성공적인보조생식술, 즉생아의출산을가능하게하는여러가지요소들에대한풍부하고귀중한자료가될수있다. 각의료기관별의치료성적은그자체로도의미가있지만성공률과성공에영향을주는요소에대한통합적인분석을위해서는국내의여러의료기관으로부터의자료를취합하여국내통계를구해보는것이의미가있을것이며, 이러한조사는국내불임환자및보조생식술시술기관의실태파악에도움을주는것은물론궁극적으로국내보조생식술에관한전체적이고일관성있는자료를장기적으로처리분석하여불임증치료의방향까지제시할수있을것으로기대된다 년 5월제정된인공수태윤리에관한선언에의하면, 인준된인공수태시술의료기관은그시술내용을연 1 회이상대한의사협회 ( 또는동협회가지정한관련학회 ) 에보고하도록되어있으며, 1994 년본학회내에인공수태시술의료기관심사소위원회가설치되었다. 그리고 2005 년부터는 생명윤리및안전에관한법률 이시행되어모든배아생성의료기관은보건복지부의인준을받도록되어있으며, 2008 년 7월현재 144 개기관이인준되어있다. 대한산부인과학회에서는 1992 년부터국내보조생식술의현황을조사하여보고하여오고있으며, 이러한조사사업의일환으로 2005 년도보조생식술현황도조사하여이에보고하게되었다. 본조사에의해얻어진국내보조생식술에대한자료는불임환자및보조생식술시술기관의실태파악에도움을주는것은물론더나아가서는시술방법의계속적인개선과발전, 부작용의감소와임신성공률의향상을도모하는데유용한자료로활용될것이다. 본조사연구는대한산부인과학회의재정지원하에수행되었으며, 실무에서많은노력을기울인대한산부인과학회의직원여러분들과국립암센터의이동옥선생님, 서울대학교병원의전성욱선생님, 자료정리에애써주신서울대학교병원전공의및전임의선생님들그리고본조사에협조하여주신국내각인공수

2 대한산부회지제 51 권제 12 호, 2008 태시술의료기관의여러분들께본위원회에서는깊은감사의말을전하고자한다. (* 는시술예가있었던기관 ) 1년간시행하는총치료주기의수에따라의료기관을분류해보면 Table 1과같다. 2008년 10월대한산부인과학회보조생식술소위원회위원장최영민 ( 서울의대 ) 간사이원돈 ( 마리아병원 ) 위원강인수 ( 관동의대 ) 권혁찬 ( 미래와희망산부인과 ) 김기철 ( 함춘여성의원 ) 김선행 ( 고려의대 ) 노성일 ( 미즈메디병원 ) 문신용 ( 서울의대 ) 문화숙 ( 문화병원 ) 민응기 ( 동국의대 ) 윤태기 ( 포천중문의대 ) 이규섭 ( 부산의대 ) 이상훈 ( 중앙의대 ) 이여일 ( 전남의대 ) 이정호 ( 계명의대 ) 전상식 ( 경북의대 ) 한혁동 ( 연세의대 ) 황경주 ( 아주의대 ) 황정혜 ( 한양의대 ) ( 이상가나다순 ) 1. 가야자모병원, * 진주 2. 강릉미즈산부인과의원, * 강릉 3. 건양대학교병원, 대전 4. 경북대학교병원, * 대구 5. 경상대학교병원, * 진주 6. 경희대학교병원, * 서울 7. 계명대학교동산병원, * 대구 8. 고려대학교병원, * 서울 9. 광주기독병원, * 광주 10. 광주미래와희망산부인과의원, 광주 11. 다나산부인과의원, 아산 12. 동국대학교경주병원, * 경주 13. 동국대학교일산병원, 고양 1. 조사대상 제 2 장조사대상및방법 본연구의목적은 2005 년 1월 1일부터 2005 년 12월 31 일까지국내에서시술된보조생식술의현황및시술성적을파악하고자하는것이다. 조사시점인 2007 년 10월당시보건복지부에배아생성의료기관으로인준되어있는 142 개의의료기관을대상으로설문지를발송하였으며 (Fig. 1), 이들기관중시술성적을보고한 88개의의료기관중시술예가있는 76개기관의결과를종합하여분석하였다. 본연구조사결과분석에포함된의료기관은다음과같으며 Fig. 1. Location of certified ART clinics in Korea,

3 최영민. 한국보조생식술의현황 : 2005 년 Table 1. Number of IVF centers according to size (total number of treatment in the year) Number of treatment started in year* Number of IVF centers 1,000 or more 5 500~ ~ ~199 7 Less than Number of IVF centers providing data for this report 88 Total number of IVF centers 동아대학교병원, * 부산 15. 동원산부인과의원, 고양 16. 동탄제일산부인과의원, 동탄 17. 마리나산부인과의원, 이천 18. 마마파파 & 베이비산부인과의원, * 울산 19. 마산삼성병원, 마산 20. 메디파크산부인과병원, * 성남 21. 미래여성병원, * 대구 22. 미래여성병원, * 대전 23. 미래와희망산부인과의원, * 서울 24. 미즈메디병원 ( 강남 ), * 서울 25. 미즈메디병원 ( 강서 ), * 서울 26. 부산대학교병원, * 부산 27. 분당서울대학교병원, * 성남 28. 분당제일여성병원, 성남 29. 사랑가득산부인과의원, * 창원 30. 사회복지법인삼성병원공익재단삼성서울병원, * 서울 31. 삼성산부인과병원, * 수원 32. 서울대학교병원, * 서울 33. 성모여성병원, * 대구 34. 세브란스산부인과의원, * 대전 35. 세화병원, * 부산 36. 시엘병원, * 광주 37. 신여성병원, * 의정부 38. 아주대학교병원, * 수원 39. 양정분산부인과의원, * 이천 40. 에덴산부인과의원, * 익산 41. 엘르메디산부인과의원, * 창원 42. 여성아이병원, * 포항 43. 연세대학교의과대학신촌세브란스병원, * 서울 44. 연세대학교원주의과대학원주기독병원, * 원주 45. 연세앙즈로산부인과의원, * 천안 46. 예일마리여성의원, * 울산 47. 운양의료재단구리장스여성의원, 구리 48. 은병원, * 광주 49. 을지대학병원, * 대전 50. 을지병원, * 서울 51. 의료법인계산재단문병원, * 전주 52. 의료법인동하의료재단한마음병원, * 창원 53. 의료법인마리아의료재단마리아병원, * 서울 54. 의료법인마리아의료재단마리아의원, * 고양 55. 의료법인마리아의료재단마리아의원, * 부천 56. 의료법인마리아의료재단마리아의원, * 평촌 57. 의료법인마리아의료재단마리아의원, * 대구 58. 의료법인마리아의료재단마리아의원, * 부산 59. 의료법인마리아의료재단마리아의원, * 대전 60. 의료법인명지의료재단명지병원, * 고양 61. 이도근산부인과의원, * 진주 62. 이화산부인과의원, * 청주 63. 이화의대부속목동병원, * 서울 64. 인정병원, * 서울 65. 인제대학교상계백병원, 서울 66. 인하대학교의과대학부속병원, * 인천 67. 일산제일병원, * 고양 68. 재단법인아산사회복지재단서울아산병원, * 서울 69. 전남대학교병원, * 광주

4 대한산부회지제 51 권제 12 호, 제일산부인과의원, * 익산 71. 제일의료재단제일병원, * 서울 72. 조선대학교병원, * 광주 73. 좋은문화병원, * 부산 74. 진산부인과의원, * 전주 75. 차산부인과의원, * 제주 76. 청아미즈산부인과의원, 아산 77. 충남대학교병원, * 대전 78. 포천중문의과대학분당차병원, * 성남 79. 포천중문의과대학차병원, * 서울 80. 프레메디산부인과의원, * 광주 81. 프리모산부인과의원, * 청주 82. 한나산부인과의원, * 서울 83. 한나여성병원, * 부산 84. 한양대학교병원, * 서울 85. 함춘여성의원, * 서울 86. 허유재병원, 고양 87. 현대병원, * 순천 88. 호산산부인과병원, * 서울 ( 가나다순 ) 2. 조사방법조사설문지를대상기관에 로발송하였으며, e- mail 로설문지를회수하였다. 발송된설문지는다음과같다

5 최영민. 한국보조생식술의현황 : 2005 년 2005 년도보조생식술시술결과보고서 REGISTRATION FORM OF ASSISTED REPRODUCTION: 2005 PLEASE READ INSTRUCTIONS AND FOOTNOTES CAREFULLY BEFORE COMPLETING THE FORMS Name of IVF Center ( 한글 ) Name of IVF Center ( 영문 ) Contact Person Name ( 한글 ) Tel Fax Address ( 한글 ) World report 에각기관의영문명이첨부됩니다. 정확한영문기관이름을적어주십시오. All data relate to treatment that were started during the calendar year, 2005, and to any clinical resulting from those treatment. Please complete as many data items as possible (n = number). If data are not available for some items, write NA. Gestational age (duration of pregnancy) should be calculated by adding 14 days (2 weeks) to the number of completed weeks between the date of fertilization (conception) and the date of completion of the pregnancy In the following tables : Standard IVF (IVF without ICSI) includes those treatment and in which fertilization occurred, or was intended to occur, in the laboratory and fresh embryos were transferred to the uterus or fallopian tubes. This heading excludes : (1) frozen/thawed embryo transfer ; and (2) microinsemination techniques. ICSI includes those treatment and that follow transfer of fresh embryos after intracytoplasmic sperm injection. Transfer after both ICSI and embryo freezing are recorded separately. GIFT includes only those and in which gametes (oocytes and sperm) were transferred to the fallopian tubes (excluding combined IVF and GIFT in the same cycle, which should be recorded in IVF category). 주의 : 본보고서에서 ART procedure 를 1) Standard IVF (IVF without ICSI), 2) ICSI, 3) ZIFT 4) GIFT, 5) FET (Frozen Embryo Transfer) after standard IVF, 6) FET after ICSI, 7) Oocyte donation 의 7 가지로구분하고있습니다. 그리고이항목들의대상은상호중첩되지않으니숫자기입시유의하시기바랍니다. If you did not perform ART practice in 2005, check here. Not done If you performed ART practice in 2005, please fill out tables below

6 대한산부회지제 51 권제 12 호, 2008 ART Report Forms, Korea 2005 Module Ia Pregnancy Outcomes in relation to Treatment Table 1a-1. Pregnancy outcomes : IVF, ICSI, ZIFT, GIFT IVF without ICSI IVF with ICSI ZIFT GIFT Initiated Cycles with oocyte retrieval attempted (aspiration) Transfers 1 embryo (oocyte 1 ) 2 embryos (oocytes) 3 embryos (oocytes) 4 embryos (oocytes) 5 embryos (oocytes) 6 embryos (oocytes) All 2 (intrauterine) Pregnancy losses 3 Pregnancies with one or more live births follow-up 4 * This table excludes in which frozen/thawed embryos were transferred. ( 이테이블에는 frozen/thawed embryo 를사용한 cycle 은포함하지않습니다.) 1 : in case of GIFT. 2 : pregnancy: Presence of an intrauterine gestational sac on ultrasound examination. ( pregnancy 는초음파에서자궁내태낭을확인한경우로제한됩니다.) 3 : pregnancy (intrauterine) 가확인되었으나임신 20 주이전 abortion 된경우. 4 : pregnancy (intrauterine) 가확인되었으나 Pregnancy outcome (abortion or delivery) 을확인하지못한임신의수

7 최영민. 한국보조생식술의현황 : 2005 년 Table 1a-2. Pregnancy outcomes after transfer of frozen/thawed embryos FET 1 : embryos obtained after IVF without ICSI Thawing Transfers 1 embryo 2 embryos 3 embryos 4 embryos 5 embryos 6 embryos All (intrauterine) Pregnancy losses 2 Pregnancies with one or more live births follow-up 3 FET: embryos obtained after IVF with ICSI 1 : FET (Frozen Embryos Transfer). 2 : pregnancy (intrauterine) 가확인되었으나임신 20 주이전 abortion 된경우. 3 : pregnancy (intrauterine) 가확인되었으나 Pregnancy outcome (abortion or delivery) 을확인하지못한임신의수. Table 1a-3. Pregnancy outcomes after oocyte donation Cycle Donation Transfers 1 embryo 2 embryos 3 embryos 4 embryos 5 embryos 6 embryos All (intrauterine) Pregnancy losses 1 Pregnancies with one or more live births 1 : pregnancy (intrauterine) 가확인되었으나임신 20 주이전 abortion 된경우. 2 : pregnancy (intrauterine) 가확인되었으나 Pregnancy outcome (abortion or delivery) 을확인하지못한임신의수

8 대한산부회지제 51 권제 12 호, 2008 ART Report Forms, Korea 2005 Module Ib Complications of Treatment Table 1c. Complications that require admission to hospital Complications Hyperstimulation syndrome Complications of oocyte retrieval Bleeding Infection All Maternal deaths related to the ART process Maternal deaths related to the pregnancy itself * If a woman had two occurrences of the same complication, in 2 different, count her twice. * If a woman had two different complications, count her in each of them

9 최영민. 한국보조생식술의현황 : 2005 년 ART Report Forms, Korea 2005 Module 2 Deliveries in relation to Treatment Table 2-1. Standard IVF (IVF without ICSI) Singleton deliveries Twin deliveries Triplet (or higher order) deliveries All deliveries * Deliveries, not babies. Percentage Table 2-2. ICSI (IVF with ICSI) Singleton deliveries Twin deliveries Triplet (or higher order) deliveries All deliveries Percentage Table 2-3. Frozen Embryo Transfer Singleton deliveries Twin deliveries Triplet (or higher order) deliveries All deliveries Percentage Table 2-4. Oocyte Donation Singleton deliveries Twin deliveries Triplet (or higher order) deliveries All deliveries Percentage Table 2-5. Induced Abortions (not SFR) Standard IVF ICSI Percentage/All Pregnancies Table 2-6. Induced Reductions (SFR) Standard IVF ICSI Percentage/All Pregnancies

10 대한산부회지제 51 권제 12 호, 2008 ART Report Forms, Korea 2005 Module 3a Perinatal Mortality in relation to Treatment and Multiple Deliveries Table 3a-1. Fresh (total aspiration following IVF and/or ICSI) (Not Deliveries, but Baby No.) Health status in the perinatal period / Number of neonates Pregnancy order Total Stillbirths Live births Neonatal deaths Unknown Singleton Twin Triplet Quadruplet or higher Unknown Total This table excludes PGD and OD This table reports number of neonates (2 for twins, 3 for triplets,..). Table 3a-2. FET (total transfer following IVF and/or ICSI) (Not Deliveries, but Baby No.) Health status in the perinatal period / Number of neonates Pregnancy order Total Stillbirths Live births Neonatal deaths Unknown Singleton Twin Triplet Quadruplet or higher Unknown Total This table excludes PGD and OD This table reports number of neonates (2 for twins, 3 for triplets,..). Table 3a-3. Oocyte donation (total transfer following IVF and/or ICSI) (Not Deliveries, but Baby No.) Health status in the perinatal period / Number of neonates Pregnancy order Total Stillbirths Live births Neonatal deaths Unknown Singleton Twin Triplet Quadruplet or higher Unknown Total This table excludes PGD This table reports number of neonates (2 for twins, 3 for triplets,..). - Live births : 20 weeks, - Stillbirths : 20 weeks - Neonatal deaths are all the deaths occurring after birth, up to 7 completed days of life. - Stillborn include deaths occurring during the delivery process

11 최영민. 한국보조생식술의현황 : 2005 년 ART Report Forms, Korea 2005 Module 3b Malformations in relation to Treatment (includes chromosomal abnormalities) Table 3b-1. Standard IVF (IVF without ICSI) number of neonates/fetuses with congenital anomalies* Technique Abortion Total Delivered** Spontaneous Induced Total IVF fresh cycle ICSI fresh cycle FET (IVF and/or ICSI) Oocyte donation PGD GIFT TOTAL *: malformations and genetic abnormalities. **: including stillbirths and those with unknown health status at birth. Delivery 20 weeks, abortion <20 weeks. Table 3b-2. ICSI (IVF with ICSI) number of neonates/fetuses with congenital anomalies* Technique Fetal losses Total Delivered** Spontaneous Induced Total IVF fresh cycle ICSI fresh cycle FET (IVF and/or ICSI) Oocyte donation PGD GIFT TOTAL *: malformations and genetic abnormalities. **: including stillbirths and those with unknown health status at birth. Delivery 20 weeks, abortion <20 weeks

12 대한산부회지제 51 권제 12 호, 2008 Table 3b-3. FET (Frozen Embryo Transfer with/without ICSI) number of neonates/fetuses with congenital anomalies* Technique Fetal losses Total Delivered** Spontaneous Induced Total IVF fresh cycle ICSI fresh cycle FET (IVF and/or ICSI) Oocyte donation PGD GIFT TOTAL *: malformations and genetic abnormalities. **: including stillbirths and those with unknown health status at birth. Delivery 20 weeks, abortion <20 weeks. Table 3b-4. Oocyte Donation number of neonates/fetuses with congenital anomalies* Technique Fetal losses Total Delivered** Spontaneous Induced Total IVF fresh cycle ICSI fresh cycle FET (IVF and/or ICSI) Oocyte donation PGD GIFT TOTAL *: malformations and genetic abnormalities. **: including stillbirths and those with unknown health status at birth. Delivery 20 weeks, abortion <20 weeks

13 최영민. 한국보조생식술의현황 : 2005 년 Please list malformations which were reported, if available: Table 3c. List of congenital anomalies (Malformations and genetic abnormalities) Baby 1. Congenital anomalies (Describe all anomalies found in each baby) Woman s Age* Technique* ART Semen / sperm* Gestational age at birth / abortion* Status*

14 대한산부회지제 51 권제 12 호, 2008 ART Report Forms, Korea 2005 Module 4 Outcomes by Maternal Age, Cause of Infertility Table 4-1. Number of oocyte retrieval and clinical by maternal age, 2005 Standard IVF (IVF without ICSI) Women s age at start of treatment Initiated Cycles with oocyte retrieval attempted Pregnancy losses 1 Pregnancies with one or more live births <25 yrs >40 1 : pregnancy (intrauterine) 가확인되었으나임신 20주이전 abortion된경우. 2 : pregnancy (intrauterine) 가확인되었으나 Pregnancy outcome (abortion or delivery) 을확인하지못한임신의수. Women s age at start of treatment Initiated Cycles with oocyte retrieval attempted IVF with ICSI Pregnancy losses 1 Pregnancies with one or more live births <25 yrs > 40 1 : pregnancy (intrauterine) 가확인되었으나임신 20주이전 abortion된경우. 2 : pregnancy (intrauterine) 가확인되었으나 Pregnancy outcome (abortion or delivery) 을확인하지못한임신의수. Women s age at start of treatment <25 yrs >40 Thawing Cycles with embryo transfer Frozen embryo transfers after IVF without ICSI Pregnancy losses 1 Pregnancies with one or more live births 1 : pregnancy (intrauterine) 가확인되었으나임신 20 주이전 abortion 된경우. 2 : pregnancy (intrauterine) 가확인되었으나 Pregnancy outcome (abortion or delivery) 을확인하지못한임신의수

15 최영민. 한국보조생식술의현황 : 2005 년 Women s age at start of treatment Thawing Cycles with embryo transfer Frozen embryo transfers after IVF with ICSI Pregnancy losses 1 Pregnancies with one or more live births <25 yrs >40 1 : pregnancy (intrauterine) 가확인되었으나임신 20주이전 abortion된경우. 2 : pregnancy (intrauterine) 가확인되었으나 Pregnancy outcome (abortion or delivery) 을확인하지못한임신의수. Women s age at start of treatment Initiated Cycles with oocyte retrieval attempted ZIFT Pregnancy losses 1 Pregnancies with one or more live births <25 yrs >40 1 : pregnancy (intrauterine) 가확인되었으나임신 20주이전 abortion된경우. 2 : pregnancy (intrauterine) 가확인되었으나 Pregnancy outcome (abortion or delivery) 을확인하지못한임신의수. Women s age at start of treatment Initiated Cycles with oocyte retrieval attempted GIFT Pregnancy losses 1 Pregnancies with one or more live births <25 yrs >40 1 : pregnancy (intrauterine) 가확인되었으나임신 20주이전 abortion된경우. 2 : pregnancy (intrauterine) 가확인되었으나 Pregnancy outcome (abortion or delivery) 을확인하지못한임신의수. Oocyte Donation Women s age at start of treatment <25 yrs >40 Initiated Cycles with oocyte retrieval attempted Pregnancy losses 1 Pregnancies with one or more live births follow-up

16 대한산부회지제 51 권제 12 호, 2008 Table 4-2. Number of oocyte retrieval and clinical by cause(s) of infertility, 2005 Standard IVF (IVF without ICSI) Cause(s) of infertility Initiated Cycles with oocyte retrieval attempted Pregnancy losses 1 Pregnancies with one or more live births Female only Male factor only Mixed male and female Unexplained Other factors - The groups in the sections on cause of infertility are mutually exclusive so each treatment cycle or pregnancy should be included in only one group. 1 : pregnancy (intrauterine) 가확인되었으나임신 20주이전 abortion된경우. 2 : pregnancy (intrauterine) 가확인되었으나 Pregnancy outcome (abortion or delivery) 을확인하지못한임신의수. Cause(s) of infertility Initiated Cycles with oocyte retrieval attempted IVF with ICSI Pregnancy losses 1 Pregnancies with one or more live births Female only Male factor only Mixed male and female Unexplained Other factors - The groups in the sections on cause of infertility are mutually exclusive so each treatment cycle or pregnancy should be included in only one group. 1 : pregnancy (intrauterine) 가확인되었으나임신 20주이전 abortion된경우. 2 : pregnancy (intrauterine) 가확인되었으나 Pregnancy outcome (abortion or delivery) 을확인하지못한임신의수. Cause(s) of infertility Female only Male factor only Mixed male and female Unexplained Other factors Initiated Cycles with oocyte retrieval attempted ZIFT Pregnancy losses 1 Pregnancies with one or more live births - The groups in the sections on cause of infertility are mutually exclusive so each treatment cycle or pregnancy should be included in only one group. 1 : pregnancy (intrauterine) 가확인되었으나임신 20 주이전 abortion 된경우. 2 : pregnancy (intrauterine) 가확인되었으나 Pregnancy outcome (abortion or delivery) 을확인하지못한임신의수

17 최영민. 한국보조생식술의현황 : 2005 년 Cause(s) of infertility Initiated Cycles with oocyte retrieval attempted GIFT Pregnancy losses 1 Pregnancies with one or more live births Female only Male factor only Mixed male and female Unexplained Other factors - The groups in the sections on cause of infertility are mutually exclusive so each treatment cycle or pregnancy should be included in only one group. 1 : pregnancy (intrauterine) 가확인되었으나임신 20주이전 abortion된경우. 2 : pregnancy (intrauterine) 가확인되었으나 Pregnancy outcome (abortion or delivery) 을확인하지못한임신의수. Frozen embryo transfers after IVF without ICSI Cause(s) of infertility Thawing Cycles with oocyte retrieval attempted Pregnancy losses 1 Pregnancies with one or more live births Female only Male factor only Mixed male and female Unexplained Other factors Frozen embryo transfers after ICSI Cause(s) of infertility Thawing Cycles with oocyte retrieval attempted Pregnancy losses 1 Pregnancies with one or more live births Female only Male factor only Mixed male and female Unexplained Other factors

18 대한산부회지제 51 권제 12 호, 2008 Table 4-3. Number of oocyte retrieval and clinical by number of embryos or oocytes transferred, 2005 Standard IVF Number of embryos transferred Transfer Pregnancy losses 1 Pregnancies with one or more live births Singleton Twin Triplet or more One Two Three Four Five Six or more 1 : pregnancy (intrauterine) 가확인되었으나임신 20주이전 abortion된경우. 2 : pregnancy (intrauterine) 가확인되었으나 Pregnancy outcome (abortion or delivery) 을확인하지못한임신의수. IVF with ICSI Number of embryos transferred Transfer Pregnancy losses Pregnancies with one or more live births Singleton Twin Triplet or more follow-up One Two Three Four Five Six or more Frozen embryo transfers after IVF without ICSI Number of embryos transferred Transfer Pregnancy losses Pregnancies with one or more live births Singleton Twin Triplet or more follow-up One Two Three Four Five Six or more

19 최영민. 한국보조생식술의현황 : 2005 년 Frozen embryo transfers after ICSI Number of embryos transferred Transfer Pregnancy losses Pregnancies with one or more live births Singleton Twin Triplet or more follow-up One Two Three Four Five Six or more ZIFT Number of embryos transferred Transfer Pregnancy losses Pregnancies with one or more live births Singleton Twin Triplet or more follow-up One Two Three Four Five Six or more GIFT Number of oocytes transferred Transfer Pregnancy losses Pregnancies with one or more live births Singleton Twin Triplet or more follow-up One Two Three Four Five Six or more

20 대한산부회지제 51 권제 12 호, 2008 ART Report Forms, Korea 2005 Module 5 Embryo Freezing and Other Special Techniques Table 5-1. Embryo freezing, 2005 Have you ever performed embryo freezing in 2005? Yes ( ), No ( ) Number of oocyte pick-up (OPU) that resulted in embryo freezing in 2005 Number of embryos that were frozen in 2005 Total number of embryos in frozen storage at present Table 5-2. Oocyte retrieval, transfer and using special techniques of sperm collection and/or assisted hatching, 2005 Selected techniques Oocyte retrieval attempted Transfer Pregnancy losses 1 Pregnancies with one or more live births Microepididymal sperm aspiration (MESA) Testicular sperm extraction (TESE) Other and unspecified techniques of sperm collection* Assisted hatching 1 : pregnancy (intrauterine) 가확인되었으나임신 20주이전 abortion된경우. 2 : pregnancy (intrauterine) 가확인되었으나 Pregnancy outcome (abortion or delivery) 을확인하지못한임신의수. *: describe: * 이상입니다. 기입하여주셔서감사드립니다. 작성시의문점이있으신경우나수정또는개선점이있으시면서울의대산부인과최영민교수 ( 전화 : , HP: , Fax: , ymchoi@snu.ac.kr) 에게연락하여주십시오

21 최영민. 한국보조생식술의현황 : 2005 년 Table 2. pregnancy in relation to treatment Treatment / IVF ICSI ZIFT GIFT FER* after IVF FER after ICSI Oocyte donation Cycles initiated (started) 7,447 8, ,630 1,590 Cycles with oocyte retrieval 6,811 7, Cycles with embryo transfer 6,506 7, ,535 1, embryo (oocyte) embryo (oocytes) 851 1, embryo (oocytes) 1,406 1, embryo (oocytes) 2,282 2, embryo (oocytes) 1,139 1, embryo (oocytes) ,960 2, (CP per retrieval, %) 28.8% 26.9% 36.4% 100.0% (CP per transfer, %) 30.1% 28.6% 40.0% 100.0% 32.5% 33.3% 40.9% Live births 1,679 1, (Live births per retrieval, %) 24.7% 22.1% 36.4% 100.0% (Live births per transfer, %) 25.8% 23.5% 40.0% 100.0% 28.7% 27.8% 39.9% *Frozen embryos replacement, estimated. 본조사에서는체외수정및자궁내배아이식술 (in vitro fertilization and embryo transfer: IVF-ET) 중난자세포질내정자주입술 (intracytoplasmic sperm injection: ICSI) 을시행한경우를분리하여조사하였으며마찬가지로냉동보존배아를이용한 IVF-ET 의경우에도 ICSI 를시행한군과시행하지않은군으로나누어조사하였다. 모든자료들은 2005 년에시작된치료주기에관련된것이고따라서 2005 년에시작된주기의결과로발생한임상적임신은모두분석에포함하였다. 임상적임신은초음파에의한태낭의확인또는소파술로얻어진임신산물등의임신의임상적증후가있는경우만을포함하고단순히혈중 β-hcg 만의상승이있었던생화학적임신은제외하였다. 임신주수는수정으로부터임신이종결된시점간의기간에 14일 (2주 ) 을더하여 completed weeks 의수로나타내었다. 그리고취합된자료들은 Microsoft 사의 Excel 프로그램과 Window 용 SPSS (version 12.0) 프로그램을이용하여분석하였다. 제3장결과분석및고찰 년도한국보조생식술시술개요 2005 년의시술예를보고해온국내의 76개의보조생식술시술기관에서총 19,149 예의보조생식술을시행하였다 (Table 2) 년에는 58개의시술기관에서 15,619 예, 2001 년 53개의시술기관에서총 14,667 예, 2002 년 69개시술기관에서 18,310 예, 2003 년에는 48개시술기관에서 14,667 예그리고 2004 년에는 65개시술기관에서 17,802 예를보고한바있다. 1 보조생식술의시술방법별분포를보면, 총 19,149 예의주기중 IVF (ICSI 를병행한경우는제외 ) 7,447 예 (38.9%), ICSI 8,262 예 (43.1%) 로 IVF 와 ICSI 시술의합이총 15,709 예 (82.0%) 로대부분의보조생식술이신선배아를이용하는것으로이루어지고있음을알수있다. 냉동보존배아이식 (frozen embryo replacement: FER) 은 3,220 예 (16.8%) 가시행되었으며, 난자공여시술 (oocyte donation) 은 208 예 (1.1%), 생식세포난관내이식술 (gamete intrafallopian transfer: GIFT) 은 1예 (0.005%), 접합자난관내이식술 (zygote intrafallopian transfer: ZIFT) 은 11예

22 대한산부회지제 51 권제 12 호, 2008 (0.057%) 였다 (Table 2, Fig. 2). 2. IVF 와 ICSI 1) 임상적임신율및생아출생률 IVF 와 ICSI 를시행받은대상환자중총 4,105 명 ( 난자채취주기당 27.7%, 배아이식주기당 29.3%) 이임상적으로임신을확인할수있었으며, 추정하여 3,441 명 ( 난자채취주기당 23.3%, 배아이식주기당 24.6%) 이생아를출산하였다 (Table 2). 2) 대상환자의연령분포및이에따른임신율 Fig. 2. Types of ART procedures. IVF 와 ICSI 를시행받은대상환자의연령분포를보면 25세미만이 0.8%, 25~29 세가 12.6%, 30~34 세가 46.5%, 35~39 세가 26.9%, 40세이상이 13.1% 로 30~39 세가전체의 73.4% 로대부분을차지하였다 (Table 3-1, Fig. 3). 난자채취주기당임상적임신율은 25세미만이 32.6%, 25~29 세가 37.8%, 30~34세가 31.9%, 35~39 세가 24.9%, 40세이상은 11.2% 로연령별로유의한차이가있었다 (P< 0.001) (Table 3-1, Fig. 4). 3) 불임원인및이에따른임신율 IVF 와 ICSI 를시행받은대상환자의적응증, 즉불임증의원인인자별분포는여성인자 (female only) 만있는경우가 41.2%, 남성인자 (male factors only) 만있는경우가 20.6%, 여성인자와남성인자가공존하는경우 (mixed male and female) 가 11.4%, 원인불명의불임증 (unexplained) 이 21.4%, 기타다른인자가 5.4% 였다 (Table 4, Fig. 5). 난자채취주기당임상적임신율은여성인자만있는경우는 28.0%, 남성인자만있는경우는 32.3%, 여성인자와남성인자가공존하는경우는 24.3%, 원인불명의불임증은 29.9%, 기타다른인자의경우 24.4% 로원인별로유 Table 3-1. Number of oocyte retrieval and clinical by maternal age Maternal age at IVF ICSI Total start of treatment OR CP (%) OR CP (%) OR CP (%) <25 25~29 30~34 35~39 > ,259 1, (27.7%) 355 (39.1%) 1,039 (31.9%) 449 (24.8%) 85 (12.2%) ,580 2,139 1, (41.35) 347 (36.6%) 1,146 (32.0%) 534 (25.0%) 131 (10.7%) 129 1,856 6,839 3,953 1, (32.6%) 702 (37.8%) 2,185 (31.9%) 983 (24.9%) 216 (11.2%) Total 6,763 1,951 (28.8%) 7,935 2,147 (27.4%) 14,698 4,128 (28.1%) Table 3-2. Number of frozen embryo replacement and clinical by maternal age Maternal age at FER without ICSI FER with ICSI Total start of treatment Transfer CP (%) Transfer CP (%) Transfer CP (%) <25 25~29 30~34 35~39 > (38.5%) 101 (39.0%) 278 (35.3%) 122 (30.7%) 19 (20.7%) (25.0%) 78 (40.2%) 269 (39.5%) 129 (30.9%) 28 (21.9%) , (33.3%) 179 (39.5%) 547 (37.3%) 251 (30.8%) 47 (21.4%) Total 1, (33.9%) 1, (35.4%) 2,978 1,031 (34.6%)

23 최영민. 한국보조생식술의현황 : 2005 년 Fig. 3. Percentage of ART users by ages: IVF & ICSI. Fig. 4. pregnancy rates by ages of women: IVF & ICSI. Table 4. Number of oocyte retrieval by cause of infertility Cause of infertility Female only Male factor only Mixed male and female Unexplained Other factors IVF ICSI Total OR CP (%) OR CP (%) OR CP (%) 3, , ,051 (29.5%) 112 (26.0%) 127 (22.8%) 573 (31.3%) 82 (26.1%) 2,314 2,501 1,065 1, (25.7%) 834 (33.3%) 267 (25.1%) 340 (27.8%) 106 (23.2%) 5,878 2,932 1,621 3, ,645 (28.0%) 946 (32.3%) 394 (24.3%) 913 (29.9%) 188 (24.4%) Total 6,693 1,945 (29.1%) 7,560 2,141 (28.3%) 14,253 4,086 (28.7%) 의하게차이가있었다 (P<0.001) (Table 4, Fig. 6). 4) 이식배아수및이에따른임신율 IVF 와 ICSI 시행주기에서이식한배아의수는 1개의경우가 10.0%, 2개인경우 14.5%, 3개인경우 21.3%, 4개인경우 32.9%, 5개인경우 14.9%, 6개이상인경우 6.3% 였다 (Table 5-1). 이식주기당임상적임신율은 1개의경우가 8.3%, 2개인경우 22.3%, 3개인경우 33.2%, 4개인경우 28.3%, 5 개인경우 29.4%, 6개이상인경우 34.9% 로이식배아수가 1개부터 3개까지는배아이식수증가에따라임상적임신율이증가하고있으며통계적으로도유의하였으나 (P< 0.001), 3개이상부터는이식수증가에따른임신율의증가와의연관성은통계적으로유의하지않았다 (P=0.558) (Table 5-1). 5) 다태아출생비율 IVF 와 ICSI 시행주기에서출생한총생아 (live birth) 중단태아의비율은 64.6% 였으며쌍태아는 34.9%, 삼태아는 0.5% 였으며사태아이상은없었다. 전체적으로쌍태아이상의다태임신의빈도가일반인구에서의쌍태아빈도가 3% 인것과 2 비교하면매우높은빈도이다 (Table 6, Fig 7). 3. 난자공여시술 (oocyte donation) 총 208 예의난자공여시술이있었으며, 193 예에서배아이식을시행하였다. 배아이식당임상적임신율은 40.9%, 생아출산율은 39.9% 였다 (Table 2). 4. 생식세포난관내이식술총 1예에서생식세포난관내이식술을시행하였다 (Table 2). 이는 2001 년의총 63예에서생식세포난관내

24 대한산부회지제 51 권제 12 호, 2008 Fig. 5. Primary diagnosis for ART procedures, IVF & ICSI. Fig. 6. pregnancy rates by cause of infertility, IVF & ICSI. Table 5-1. by number of embryos transferred Number of embryos transferred Standard IVF IVF with ICSI Total One Two Three Four Five Six or more Transfer ,384 2,219 1, (%) 47 (10.4%) 210 (24.9%) 464 (33.5%) 451 (20.3%) 307 (29.5%) 143 (40.3%) Transfer 918 1,128 1,524 2, (%) 66 (7.2%) 230 (20.4%) 501 (32.9%) 819 (36.2%) 291 (29.3%) 158 (31.2%) Transfer 1,369 1,973 2,908 4,480 2, (%) 113 (8.3%) 440 (22.3%) 965 (33.2%) 1,270 (28.3%) 598 (29.4%) 301 (34.9%) Total 6,296 1,622 (25.8%) 7,332 2,065 (28.2%) 13,628 3,687 (27.1%) Table 5-2. by number of frozen embryos transferred (FER) Number of embryos transferred Standard IVF IVF with ICSI Total One Two Three Four Five Six or more Transfer (%) 22 (17.5%) 88 (28.9%) 196 (40.1%) 120 (37.3%) 69 (34.8%) 20 (29.9%) Transfer (%) 13 (15.3%) 83 (30.5%) 192 (39.3%) 119 (30.7%) 62 (32.3%) 22 (31.4%) Transfer (%) 35 (16.6%) 171 (29.6%) 388 (39.7%) 239 (33.7%) 131 (33.6%) 42 (30.7%) Total 1, (34.2%) 1, (32.8%) 3,002 1,006 (33.5%) 이식술을시행한것에비하면많이감소한수치로 2004 년에는 2예시행하였다. 1 접합자난관내이식 (ZIFT) 은총 11 예가시행되었으며이식주기당임신율과생아출산율은모두 40.0% 였다 (Table 2). 5. 냉동보존배아이식 (frozen embryo replacement) 2005 년에총 3,220 예의냉동보존배아이식시도가있었으며, 이중총 3,051 예에서냉동보존배아이식을시행하였 다. 배아이식주기당임상적임신율은 32.9% 생아출산율은 28.3% 였다 (Table 2) 년에는총 2,653 예의냉동보존배아이식시도가있었으며, 이중총 2,577 예에서냉동보존배아이식을시행하여배아이식주기당임상적임신율은 35.0%, 생아출산율은 27.0% 를보였다 년도의배아이식주기당임상적임신율및생아출산율은 2004 년도와통계적으로유의한차이가없었다 (P=0.107 and P=0.309, respectively) 년도의배아이식주기

25 최영민. 한국보조생식술의현황 : 2005 년 당임상적임신율및생아출산율은 2003 년도 (30.1% 및 23.3%) 와유의한차이가있었음을보고한바있다 (P= and P=0.004, respectively). 1 냉동보존배아이식시도를시행한환자의연령분포를보 Fig. 7. Pluralities among live births: IVF & ICSI. 면 25세미만이 0.7%, 25~29 세가 15.2%, 30~34 세가 49.3%, 35~39 세가 27.4%, 40세이상이 7.4% 로 30~39 세가전체의 76.7% 로대부분을차지하였다 (Table 3-2). 이식주기당임상적임신율은 25세미만이 33.3%, 25~29 세가 39.5%, 30~34세가 37.3%, 35~39 세가 30.8%, 40세이상은 21.4% 로연령별로유의한차이가있었다 (Table 3-2). 이식한배아의수는 1개의경우가 7.0%, 2개인경우 19.2%, 3개인경우 32.6%, 4개인경우 23.6%, 5개인경우 13.0%, 6개이상인경우 4.6% 였다 (Table 5-2). 이식주기당임상적임신율은이식한배아의수가 1개의경우는 16.6%, 2개인경우 29.6%, 3개인경우 39.7%, 4개인경우 33.7%, 5개인경우 33.6%, 6개이상인경우 30.7% 로배아이식수에따라유의하게다른임신율을보였다 (P<0.001, Table 6. Deliveries in relation to treatment IVF Number Percentage ICSI Number Percentage FER Number Percentage Oocyte donation Number Percentage Singleton Twin deliveries Triplet deliveries All deliveries 366 (65.1%) 678 (64.3%) 225 (70.1%) 20 (52.6%) 193 (34.3%) 371 (35.2%) 90 (28.0%) 17 (44.7%) 3 (0.5%) 5 (0.5%) 6 (1.9%) 1 (2.6%) 562 1, Table 7. Oocyte retrieval, transfer and using special techniques of sperm collection and/or assisted hatching Oocyte retrieval Selected techniques attempted Microepididymal sperm aspiration (MESA) Testicular sperm extraction (TESE) Other and unspecified techniques of sperm collection* Transfer (%*) Pregnancies with live births (%*) (25.3%) 18 (19.8%) (34.0%) 128 (24.2%) (45.5%) 3 (36.4%) Assisted hatching 2,163 2, (25.1%) 383 (17.7%) *Per oocyte retrieval cycle

26 대한산부회지제 51 권제 12 호, 2008 Table 8. Comparison with data from international registers Korea, 2005 US, ESHRE, IVF Cycles with oocyte retrieval 6,811 85,713* 104,739 Cycles with embryo transfer 6,506 78,797* 92,355 pregnancy (CP) 1,960 33,101* 27,845 CP per retrieval (%) 28.8% 38.6%* 26.6% CP per transfer (%) 30.1% 42.0%* 30.1% Live birth per retrieval (%) 24.7% 34.3%* ICSI Cycles with oocyte retrieval ,857 Cycles with embryo transfer 7, ,276 pregnancy (CP) 2,145 42,040 CP per retrieval (%) 26.9% 27.1% CP per transfer (%) 28.6% 29.8% Live birth per retrieval (%) 22.1% FER after IVF Cycles with embryo transfer 3,051 18,812 64,147 with/without ICSI pregnancy 1,004 6,721 12,275 CP per transfer 32.9% 35.7% 19.1% Live birth per transfer 28.3% 28.0% *Including ICSI. US: United states, ESHRE: European Society for Human Reproduction and Embryology. Table 5-2). 6. 수술적방법에의한정자채취와보조부화술 2005 년도에시행된 MESA (microsurgical epididymal sperm aspiration) 는총 91예이고난자채취주기당임신율은 25.3%, 난자채취주기당생아출생률은 19.8% 였다. TESE (testicular sperm extraction) 는 529 예에서시행되었고난자채취주기당임신율은 34.0% 였고난자채취주기당생아출생률은 24.2% 였다. 보조부화술은 2,163 예에서시행되었고난자채취주기당임신율은 25.1%, 난자채취주기당생아출생률은 17.7% 였다 (Table 7). 7. 외국보고서와의비교보고된 2005 년도결과를국제데이터와비교한것은 Table 8과같다. 미국의경우 Centers for Disease Control and Prevention (CDC) 에서보고한 2005 년 ART success rates: National summary and fertility clinic report 3 가최근보고이며, ESHRE (European Society for Human Reproduction and Embryology) 의최근보고인 2004 년보조생식술결과는 4 유럽 29개국의데이터를분석한것이다. ICSI 를포함한신선배아를이용한주기를비교할때, 미국의경우배아이식주기당임상적임신율이 42.0% 로우리나라의 29.3% 에비해유의하게높았으며 (P<0.001), ESHRE 보고의경우배아이식주기당임상적임신율은 29.9% 로우리나라와통계적으로유의한차이가없었다 (P=0.136, Table 8). 냉동보존배아이식의경우에서는미국의경우배아이식주기당임상적임신율이 35.7% 로우리나라의 32.9% 에비해통계적으로유의한차이는있었으나 (P=0.003) 그차이는크지않은반면, 우리나라와유럽의결과를비교할때 ESHRE 에서보고한유럽의냉동보존배아이식의경우배아이식주기당임상적임신율이 19.1% 로우리나라의결과에비해통계적으로유의하게낮았다 (P<0.001, Table 8). 각기관의결과는같은해의결과가아닌가장최근보고된결과로적어도국내에서의결과가국제적결과보다크게나쁘지않다는것을보여준다

27 최영민. 한국보조생식술의현황 : 2005 년 제4장맺음말 2005 년한국의보조생식술현황은기존의 2004 년까지의보고와큰차이는없었다. 주목할만한사실은국외의경우신선배아를이용한경우에비해냉동배아를이용한경우의임신율이월등히떨어지는것을보여준반면, 우리나라의경우냉동배아를이용한경우가오히려신선배아를이용한경우보다 (Table 2) 임상적임신율이통계적으 로유의하게높았으며 (P<0.001), 이런비슷한경향은 2004 년보조생식술현황보고때도나타난바있다. 1 특별히냉동보존배아이식의임신율에있어국외보고들과차이가나타난원인이무엇인지에대해선더연구해볼필요가있을것으로사료된다. 그리고보조생식술의성공에영향을줄수있는중요한요인으로여성의나이, 불임의원인, 배아이식수등이포함된다는것은이전결과들과같았다고결론지을수있겠다. 1. 인공수태시술의료기관심사소위원회. 조사보고서한국보조생식술의현황 : 2004 년. 대한산부회지 2007; 50: Cunningham FG, Bloom SL, Leveno KJ, Gilstrap III L, Hauth JC, Wenstrom KD. Williams Obstetrics. 22th ed. New York: McGraw-Hill; 참고문헌 3. Centers for Disease Control and Prevention (U.S.), Division of Reproductive Health, Society for Assisted Reproductive Technology, American Society for Reproductive Medicine Assisted reproductive technology success rates national summary and fertility clinic reports. somerville, MA: RESOLVE; Anderson AN, Goossens V, Ferraretti AP, Bhattacharya S, Felberbaum R, de Mouzon J, et al. Assisted reproductive technology in Europe, 2004: Results generated from European registers by ESHRE. Hum Reprod 2008; 23: