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1 Korean J Clin Lab Sci. 209;5(3): Korean Society for Clinical Laboratory Science ORIGINAL ARTICLE Density and Distribution of the Mosquito Population Inhabiting Jeju Region, 208 Min Young Seo, Kyoung A Chung Department of Clinical Laboratory Science, Gwangju Health Science University, Gwangju, Korea 제주지역에서식하는모기개체군밀도와분포, 208 서민영, 정경아 광주보건대학교임상병리과 ARTICLE INFO Received August 9, 209 Revised st August 27, 209 Revised 2 nd September 4, 209 Revised 3 rd September 5, 209 Accepted September 5, 209 Key words Flavivirus Jeju region Mosquitoes ABSTRACT In order to investigate the density of seasonal incidence of mosquitoes, a vector of infectious diseases in Jeju region, this study collected mosquitoes using a Black light trap () and Biogents Sentinel 2 Mosquito Trap (BG), dividing the region into cattle sheds, habitats for migratory birds, and the downtown area, twice a month for 9 months from March through November 208. Then, this study conducted identification and classification and checked for the presence of Flavivirus using reverse transcription polymerase chain reaction (RT-PCR). As for the mosquito population,,847 mosquitoes (six genera, 2 species) were collected. The places where most mosquitoes were collected were copses near craft workshops in habitats for migratory birds and Jungang-dong in the Seogwipo downtown area. For the population, Culex pipiens pallens was the dominant species (76.9%), followed by Aedes albopictus (8.9%). Most of the population was collected in June, followed by August and October. This study conducted a RT-PCR test with,847 collected mosquitoes, which were divided into 50 pools if they had Flavivirus. All turned out to be negative. However, the results of the investigation show the presence of Culex tritaineniorhychus, Aedes albopictus, and Anopheles sinensis and can be used as a basis for the comprehensive prevention management of mosquitoes. Copyright 209 The Korean Society for Clinical Laboratory Science. All rights reserved. 서론 산업혁명이후경제적활동에따른화석연료의사용은온실가스의증가를야기했고, 지구의평균온도를지속적으로상승시켰다. 지구기후변화는기상재해, 생태계파괴와같은환경위기를초래하고, 인류의지속가능한삶을위협하는요인이되고있다 []. 지난 00년간지구의평균온도가.7 C 상승하였고, 강수량 * Corresponding author: Kyoung A Chung Department of Clinical Laboratory Science, Gwangju Health Science University, Bungmun-daero, Gwangsan-gu, Gwangju 62287, Korea kachung@ghu.ac.kr * ORCID: 도연간 230 mm가증가하였으며, 기온상승은생태계의변화를초래하여통제가능했던미생물의재등장으로신종질병증가와확산, 인수공통감염병의발생의요인이될수있는매개곤충의발생밀도를높이며도시화와산업화에의해서이루어진환경에적응력이있는특정한생물군의유입과정착으로이어지게되었다 [2]. 매개곤충은생활사주기에적합하고기후요인이가장적합한지역에서번식과활동을하는데기온의증가는일반적으로매개곤충과병원체의증식에큰영향을미치고, 질병매개곤충의생존에영향을미치는습도와상호작용을한다 [3]. 지구온난화에의한기후와환경변화로인해여름철뿐만아니라겨울에도모기의발생과흡혈활동이나타나고지역에따 pissn eissn This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
2 Korean J Clin Lab Sci. Vol. 5, No. 3, September 라모기로인한피해가발생되며, 주변변화환경에빠른적응력을보인모기는다양한서식지와개체수를늘려가며모기로인한질병문제가발생되고있다 [4]. 모기는전세계적으로 3,26종이분류및보고되어있으며, 국내에는 9속 56종이기록되어있다. 유럽과미국에는모기를매개로한웨스트나일열, 황열, 뎅기열과같은감염성질환으로많은환자가발생되었으며, 이와같은해외감염성질환이언제든지국내로유입가능하며, 해외여행자들에의한감염성질환의국내유입사례가있어국내유입감시가지속적으로필요한실정이다 [2]. 국내서식하는모기종에서작은빨간집모기 (Culex tritaeniorhynchus) 는일본뇌염 (Japanese encephalitis) [5], 중국얼룩날개모기 (Anopheles sinensis) 는말라리아 (Malaria) 와사상충증 (Filariasis), 토고숲모기 (Ochlerotatus togoi) 는사상충증을전파하는매개체로알려져있다 [6]. 그외국내모기에서분리한병원균으로는금빛숲모기 (Aedes vexans nipponii) 에서 Getah Virus 를, 흰줄숲모기 (Aedes albopictus) 에서 flavivirus 가분리되었다. 아직바이러스가숙주및매개모기에서분리되지않았지만모기매개질병을가지면서국내서식하는매개종으로흰줄숲모기 (Aedes albopictus) 는뎅기열 (Dengue fever) [7] 을빨간집모기 (Culex pipiens complex), 일본숲모기 (Ochlerotatus japonicus), 등줄숲모기 (Ochlerotatus dorsalis), 금빛숲모기 (Aedes vexans), 흰줄숲모기 (Aedes albopictus) 는웨스트나일뇌염 (West Nile encephalitis) [2] 을일으킨다. 국내에서식하는주요모기의서식처및휴식처는작은빨간집모기 (Culex tritaeniorhynchus) 와중국얼룩날개모기 (Anopheles sinensis) 는논, 맑은물이고인웅덩이나수로이고휴식지로는대다수옥외장소이다. 토고숲모기 (Ochlero tatustogoi) 는해변가바위나웅덩이에고여있는물과낙엽등에서식하며, 빨간집모기 (Culex pipiens complex), 지하집모기 (Culex pipiens moelstus) 는도시및농촌의주거지역에서서식하며흡혈활동한다. 이들은열대, 아열대지역에주로서식하는데지구온난화로인한기상변화는기후대경계지역에서변이종의출현으로인하여질병전파가가속될수있다. 큰검정들모기 (Armigeres subalbatus) 는재래식화장실, 돼지나소분뇨통에서주로서식하고있다 [4, 8]. 모기는각종마다출혈시기, 서식처, 흡혈활동등이다르기때문에, 지역별로나타나는모기의종류및우점종의파악은모기관리에있어서매우중요하다 [9]. 제주도는한반도의최남단에위치하며수리적으로북위 , 동경 에해당되며일반적인기후분류상아열대기후대에서온대기후대로의전이지대에위치하고있다. 태양고도가연중높아태양의순복사량이에너지수지상과잉이시작되는위도대이며여름에는태양의북상으로열대기단의영향권에들기도한다. 제주도주변및남해표층수온은 970년대 8 9 C 사이지만, 최근 0년 ( 년) 9.5 C에가까운값을보이고있다. 한반도주변해역전체로써는최근 4년간약.3 C 상승하는추세이며, 남해에서.29 C의상승폭을보이고있다 [0]. 본조사는급변하는기후변화및국제교류등에따른매개체감염질병의증가에따라, 제주지역질병매개모기종의분류 동정및개체수조사, 지역별분포와바이러스병원체감염여부등을조사하여모기방제및감염병방역대책수립등에활용하고자실시하였다. 재료및방법. 채집기간및장소제주지역을대상으로 208년 3월부터 월까지 9개월간감염병매개체인모기를대상으로매개체전파질환의조사 감시를실시하였다. 채집방법으로 Kim 등 [] 의연구방법을일부응용하여매개모기의발생동향과병원체를감시하기위해도심 3지점과철새도래지 3지점및축사 지점에서월 2회모기를 Black light trap () 과 Biogents Sentinel 2 Mosquito Trap (BG) 을이용하여모기를채집하였다. 각지점별로모기가출현하는일몰전부터다음날일출전까지 과 BG를이용하여모기를채집하였다 (Table ). 2. 분류및동정실험실로가져온채집모기는분류및동정전까지냉동보관하며살아있는모기를치사시킨후형태보존하였다. 모기종에따른분류 동정은 Tanaka 등 [2] 과 Hong [3] 의분류키를이용하여형태적으로분류하였고실체현미경하 (SZ6, Olympus, Japan) 에서관찰하였다. 3. 모기로부터병원체확인병원체검출방법은 Lee와 Hwang [4] 과 Lee 등 [5] 의연구방법을인용하여시행하였다. 채집된모기를종별로분리한후 2 ml tube에최대 50마리의모기와 PBS (phosphate buffered
3 338 Min Young Seo and Kyoung A Chung. Density and Distribution of the Mosquito Population Inhabiting Jeju Region, 208 Table. Area and environment for collecting mosquitoes Survey area Survey environment Collection area Latitude and longitude Investigation method Time of investigation Frequency of investigation Remarks Jeju-City Cattle shed Aladong N/ E Bird sanctuary Gujwa-eub hadoli (gongbang) N/ E Gujwa-eub hadoli (gongbang-around) N/ E Gujwa-eub hadoli (welfare hall) N/ E Seogwipo-City Downtown Youngchun-dong (resid center) N/ E Jongang-dong (resid center) N/ E Seo hong-dong (ecological park) N/ E BG BG March November Twice a month Classification identification and pathogen identification Table 2. Primer sequence of PCR for diagnosis of flavivirus Name Polarity Sequence (5 to 3 ) FL-F Sense GCC ATA TGG TAC ATG TGG CTG GGA GC FL-R3 Antisense GTK ATT CTT GTG TCC CAW CCG GCT GTG TCA TC FL-R4 Antisense GTG ATG CGR GTG TCC CAG CCR GCK GTG TCA TC saline).2 ml을넣고 0초씩간격을두고 30초간 2번씩 6,500 rpm으로자동파쇄기 (Precellys 24, Bertin Tech, France) 를이용하여파쇄하였다. tube를파쇄기에서꺼낸후얼음에 5분정치시킨후냉장원심분리기에상기 tubes를넣고, 3,000 rpm 에서 분간원심분리하였다. 상층액을분리하여유전자를추출하였다. 병원체의검출방법은 flavivirus의비구조단백질 5 유전자 (nonstructural protein 5; NS 5 gene) 의공통부위를표적으로하는병원체의종특이적프라이머의염기서열은 Chung 등 [6] 이보고한염기서열을인용하여시행하였다 (Table 2). RNA 분리는 QlAamp Viral RNA Mini kit (Qiagen, Hilden, Germany) 를사용하였으며, 병원체조사를위한 PCR 수행은 flavivirus 검사를위한 One Step SYBR Primescript RT-PCR kit (Takara, Kusatsu, Japan) 사용후 PCR을하였다. 결과. 매개모기채집결과전체채집된모기개체수는 6속 2종으로,847마리가채집되었다. 채집지역별로축사, 철새도래지, 서귀포순으로채집되었다. 축사에서는 40마리 (Trap index, TI 2.4) 가채집되었다. 철새도래지에서는복지관에서 92마리 (TI 5.4), 공방에서 4 마리 (TI 6.7), 공방주변잡목림에서 60마리 (TI 9.4) 가채집되 었다. 서귀포에서는영천동에서 79마리 (TI 4.6), 중앙동에서,74마리 (TI 69.), 생태공원에서 90마리 (TI.2) 가채집되었다. 철새도래지에서는공방주변잡목림에서, 서귀포도심에서는중앙동에서가장많은모기가채집되었다 (Table 3). 채집된모기의종류는빨간집모기 (Culex pipiens pallens) 가,42마리, 흰줄숲모기 (Aedes albopictus) 가 64마리, 중국얼룩날개모기 (Anopheles sinensis) 가 92마리, 토고숲모기 (Ochlerotatus togoi) 가 60마리, 큰검정들모기 (Armigeres subalbatus) 가 57마리, 작은빨간집모기 (Culex tritaeniorhynchus) 가 30마리, 금빛숲모기 (Aedes vexans nipponii) 가 2마리, 반점날개집모기 (Culex bitaeniorhynchus) 가 4마리, 반점날개늪모기 (Mansonia uniformis) 가 2마리, 가중국얼룩날개모기 (Anopheles sineroides) 와줄다리집모기 (Culex vagans) 가각각 마리씩채집되었다. 가장많은개체수로는빨간집모기 (Culex pipiens pallens) 가 76.9% 로우점종이었고, 그다음으로흰줄숲모기 (Aedes albopictus) 가 8.9% 로나타났다 (Table 4, Figure ). 가장많은개체수가채집된시기는 6월에 436마리, 8월에 396마리, 0월에는 290마리순으로나타났다. 빨간집모기 (Culex pipiens pallens) 는 3월부터나타나기시작하여 6월 (TI 23.5) 에최고조에이르며, 흰줄숲모기 (Aedes albopictus), 중국얼룩날개모기 (Anopheles sinensis) 는 6월에나타나기시작
4 Korean J Clin Lab Sci. Vol. 5, No. 3, September Table 3. Total number of female mosquitoes collected and trap indices at cattle sheds, bird sanctuary and downtown with traps in Jeju Locations Trap Number of females Trap nights Trap index* Jeju-city Cattle shed Bird sanctuary Gongbang Gongbang-around BG Welfare hall Seogwipo-city Youngchun-dong Jongang-dong, Ecological park BG Total, *Trap index is number of female mosquitoes per trap nights. Table 4. Seasonal prevalence of female mosquitoes collected in Jeju N (%) Mar Apr May Jun Jul Aug Sep Oct Nov Total* Culex pipiens pallens 9 (.03) 66 (3.57) 4 (2.22) 329 (7.8) 56 (8.45) 242 (3.) Aedes albopictus (2.0) (0.6) (2.82) Armigeres subalbatus (.3) (0.38) (.4) Aedes vexans nipponii 2 7 (0.38) Ochlerotaus togoi (0.54) (2.0) (22) Anopheles sinensis (0.6) (.08) (2.87) Anopheles sineroides Culex vagans Culex bitaeniorhynchus 3 (0.6) Mansonia uniformis 2 Culex tritaeniorhynchus 2 7 (0.92) Ochlerotatus dorsalis 2 Total (.03) (4.28) (2.38) (23.6) (0.94) (2.44) 2 (6.06) 39 (2.) 5 (0.27) 0 (0.54) 70 (9.20) 255 (3.8) 8 (0.97) 2 4 (0.22) 0 (0.54) 290 (5.70) 20 (0.88) 7 (0.38) 2 2 (.42) 42 (76.9) 64 (8.88) 57 (3.09) 2 (0.65) 60 (3.25) 92 (4.98) 4 (0.22) 2 30 (.62) 3 (0.6) 847 (00) *Percentage for total collected in each month. 에서 8월 (TI 3.7, 3.8,) 에최고조에이르고, 토고숲모기 (Ochlerotatus togoi) 는 4월에나타나 6월 (TI 2.6) 에최고조에이르고작은빨간집모기 (Culex tritaeniorhynchus) 는 7월에나타나 8월에최고조에이르렀다 (Table 5). 2. 병원체감시 208년 3월부터 월까지채집한모기,847마리를 50 pools로나누어 flavivirus 보유여부를 RT-PCR 검사하였으나, 모두음성으로확인되었다 (Figure 2). 고찰기후변화에따른지구온난화와교통수단의발달로인해서국제적교류의증가는사람과매개체와의접촉빈도가높아지게됨으로써매개질환전파가더욱가능해졌으며, 과거한특정지역에국한되어발생하던감염질환이최근에는빠른속도로전세계여러나라로전파됨으로써대유행을유발하고있다 [4]. 여름은물론, 겨울철에도기온상승과지역에따라강우량증가로인해모기의발생과모기의흡혈활동이증가되고있으며모기에따른피해도계속늘어나고있다. 환경변화에따른모기종
5 340 Min Young Seo and Kyoung A Chung. Density and Distribution of the Mosquito Population Inhabiting Jeju Region, 208 의변화는아직우리나라에서발견되지않은모기또는잠재적인외래종발생의가능성과외래종의토착가능성이있으므로지속적인모니터링을통한우리나라에존재하는질병매개모기의발생조사는필연적이라고판단된다 [7]. 208년 3월부터 월까지제주지역의총 7개지점에서채집된암컷모기의총수는,847 마리로총 6속 2종이었다. 207 년 5속 7종 6,042마리에비해종은늘었지만개체수가감소하였는데이는 2번의태풍과집중호우, 짧은장마로인한 30 C를넘는폭염과열대야로인하여유충과성충의대사활동성과생존률감소로채집량에변화를가져온것으로사료된다 [4]. 그러나 207년에비해모기종은다양하게되었는데이는기후변화에따른외래종의유입과토착가능성종간교배로인한변이종의발견등다양성에대한조사가필요하다고사료된다. 지역적으로서귀포에서는중앙동에서,74마리 (TI 69.), 철새도래지에서는공방주변잡목림에서 60마리 (TI 9.4) 로가장많은모기가채집되었다. 이러한결과는 207년 Lee와 Hwang [4] 영천동에서의 TI 6.3, 중앙동 72.8로본결과와유사한결과를보인데이는중앙동은서귀포시내중심지역에위치하여상설시장을비롯한많은상가가밀집된지역으로서건물의난방시설, 지하구조물, 하수시설과많은사람의왕래가있어모기채집수가많은것으로사료된다. 생태공원은 207년 59.8에비해서 208년에는.2로낮은결과를보였다. 영천동은같은도심이지만중앙동에비해고도가높고주변환경이한라산국립공원과가까이있으며생태공원은국민들이건강에대한관심이높아짐으로인해생활체육공간에대한주변정비사업과집중적인방역활동에대한영향으로추측된다. 세종지역에서는채집모기가제일적은지역은생태공원으로본조사와 Figure. Female mosquitoes collected in Jeju. (A) Culex pipiens pallens, (B) Aedes albopictus, (C) Anopheles, (D) Culex tritaeniorhynchus, (E) Armigeres subalbatus. Figure 2. Result of RT-PCR. Figure 2 showed result for flavivirus detection from female mosquitoes collected in Jeju. Abbreviations: M, DNA marker; N, negative; P, positive; 5, sample. Table 5. Seasonal TI of female mosquitoes collected in Jeju Mar Apr May Jun Jul Aug Sep Oct Nov Total* Culex pipiens pallens Aedes albopictus Armigeres subalbatus Aedes vexans nipponii Ochlerotatus togoi Anopheles sinensis Anopheles sineroides Culex vagans Culex bitaeniorhynchus Mansonia uniformis Culex tritaeniorhynchus Ochlerotatus dorsalis *TI is number of female mosquitoes per trap nights.
6 Korean J Clin Lab Sci. Vol. 5, No. 3, September 유사한결과가나와방역활동등과같은환경적요인을뒷받침하고있다 [5]. 본조사에서축사는 TI 2.4로가장낮았는데, 207년축사 TI 6.4 보다더낮게나타났으며이는구제역등감염병발생에대한적극적인예방활동이이루어진것으로생각된다 [4]. 본조사에서가장많은모기개체수로는빨간집모기 (Culex pipiens pallens) 가모기총수,847 중,42마리로 76.9% 를차지하고있어우점종으로나타났다. 그다음으로흰줄숲모기 (Aedes albopictus) 가 8.9%, 중국얼룩날개모기 (Anopheles sinensis) 가 5.0% 순으로나타났다. 그리고일본뇌염을매개하는것으로알려진작은빨간집모기 (Culex tritaeniorhynchus) 는.6% 을차지하는것으로나타났다. Lee와 Hwang [4] 의 207년보고에서는빨간집모기 (Culex pipiens pallens), 흰줄숲모기 (Aedes albopictus) 순으로점유율은같았으나 3번째로는큰검정들모기 (Armigeres subalbatu) 가차지하여본조사와차이가있었으며, 작은빨간집모기 (Culex tritaeniorhynchus) 는적은채집량 (0.4%) 을나타내본연구와일치하였다. 세종시의경우는빨간집모기 (Culex pipiens pallens) 36.3%, 금빛숲모기 (Aedes vexans nipponii) 30%, 중국얼룩날개모기 (Anopheles sinensis) 8.8%, 작은빨간집모기 (Culex tritaeniorhynchus) 7.0%, 큰검정들모기 (Armigeres subalbatu).7% 순으로나타나우점종을나타내는빨간집모기 (Culex pipiens pallens) 를제외하고채집되는량이차이가나타나며작은빨간집모기 (Culex tritaeniorhynchus) 이가차지하는비율도높게나타났는데이는세종시가가지고있는기후와주변환경의차이에의해나타나는종이다른것으로사료된다. 우점종을나타내는빨간집모기 (Culex pipiens pallens) 는다른모기종에비해온난화로인하여다양한산란장소와서식처를가지고있어서서울, 대구, 부산, 울산등여러지역에서의우점종을차지하고있어본조사와일치하였다 [8]. 다른지역에비해제주에서낮은채집률을보인작은빨간집모기 (Culex tritaeniorhychus) 는제주지역이다른지역에비해주서식처인논이없는특징과관련있다고사료된다 [4]. 빨간집모기 (Culex pipiens pallens) 는 6월 (329마리), 흰줄숲모기 (Aedes albopictus) 는 8월 (52마리) 에가장많이채집되었다. 시기적으로는 6월, 8월, 0월순으로모기가많이채집되었다. 모기는주로기온이올라가면서강우량이상대적으로적은 4월부터활동하기시작하여기온이올라가는 6월에발생밀도가증가하며높은강수량과온도가떨어지는장마철에발생밀도가떨어지다가다시대기온도가상승하면개체밀도가다시높게증가하는추세를보이고있다 [9, 9]. Lee와 Hwang [4] 의조사에서 207년에는 8월 6월 5월의순이고 206년도에는 8월, 9월, 0월순으로많이채집되어차이가나타났는데이는태풍과고온등을비롯한기후변화요인에따른현상으로생각된다. 7월에는 6월과 8월사이에모기발생밀도가감소된경향을보이는데이는태풍으로인한집중호우, 홍수등으로유충이소실되는등유충생존조건이악화되어모기개체수에직접적인영향을준것으로사료된다. 강수량은모기의개체수와밀접한관계가있어적정한강수량의증가는서식지제공으로매개체증가로인한질병매개와관련되며폭우와같은과도한강수량증가는수인성질환을일으키는요인으로작용하기도하지만물에있던유충들이씻겨내려가게하여곤충매개체를감소시키는효과를나타내기도한다 [9, 20]. 채집방법에따른모기채집율은같은지역 ( 공방 ) 에서, BG 를사용하여채집한결과 은 6.7, BG 9.4로 BG방법에서보다높은모기채집율을보였으며 Lee와 Hwang [4] 의조사에서는공항과항만에서만두가지방법을다사용했는데공항에서 은.4과 BG는 39.5, 항만에서는 은 4.8, BG는 7.7로차이가있어본조사와일치하였지만본조사와 Lee와 Hwang [4] 에서도일부에서만시행하여채집방법에따른효율성을정확하게비교할수는없지만앞으로좀더많은곳에서두가지방법을시행하여더효과적인모기채집방법선정에고려해야될사항으로생각된다. Flavivirus 는플라비비리데과 (Flaviviridae family) 의하위생물학적분류인속 (genus) 을지칭하며지카바이러스 (Zika virus), 뎅기바이러스 (Dengue virus), 일본뇌염바이러스 (Japanese encephalitis virus), 황열바이러스 (Yellow fever virus), 웨스트나일바이러스 (West Nile virus) 및세인트루이스뇌염바이러스 (St. Louis encephalitis virus) 등이포함되며, 바이러스에감염되면발열, 두통, 근육통, 오한, 구토, 설사등의공통증상이나타난다고특히아시아국가들의경우에는, 감염빈도가비교적높은지카바이러스, 뎅기바이러스및일본뇌염바이러스감염에대한구별이중요하다 [2]. 208년 3월부터 월까지채집한모기,847마리를 50 pools 로나누어 flavivirus 보유여부를 real-time RT-PCR 검사하였으나, 모두음성으로확인되었다. 207년조사와세종조사에서도모두음성으로나왔다 [4, 7]. 이번에채집된모기를이용한바이러스감염여부에서음성으로나와바이러스는검출은확인되지않았지만제주지역에일본뇌염를일으키는작은빨간집모기 (Culex tritaeniorhychus), 뎅기열을일으키는흰줄숲모기 (Aedes albopictus), 말라리아 (Malaria) 와사상충증 (Filariasis) 을일으키는중국얼룩날개모기 (Anopheles sinensis),
7 342 Min Young Seo and Kyoung A Chung. Density and Distribution of the Mosquito Population Inhabiting Jeju Region, 208 사상충증을일으키는토고숲모기 (Ochlerotatus togoi), 웨스트나일뇌염을일으키는빨간집모기 (Culex pipienscomplex), 일본숲모기 (Ochlerotatus japonicus), 등줄숲모기 (Ochlerotatus dorsalis), 금빛숲모기 (Aedes vexans), 흰줄숲모기 (Aedes albopictus) 가조사결과서식하는것으로확인되어향후모기에대한종합적인방제관리와감염병예방에대한대책수립의기초에활용할수있다고사료된다. 요약 본조사는제주지역감염병매개체인모기를대상으로계절적발생밀도를조사하기위해축사, 철새도래지, 도심으로나누어 208년 3월부터 월까지 9개월간월 2회모기를 과 BG 를이용하여모기를채집한후분류및동정을실시하고, RT-PCR 을이용하여 flavivirus의보유여부를확인하였다. 채집된모기는 6속 2종으로,847마리가채집되었다. 채집장소중에서철새도래지에서는공방주변잡목림, 서귀포도심에서는중앙동에서가장많이채집되었다. 가장많은개체수로는빨간집모기 (Culex pipiens pallens) 가 76.9% 로우점종이었고, 그다음으로흰줄숲모기 (Aedes albopictus) 가 8.9% 로나타났다. 가장많은모기개체수가채집된시기는 6월, 8월, 0월순으로나타났다. 채집한모기,847마리를 50 pools로나누어 flavivirus의보유여부를 RT-PCR 검사하였으나, 모두음성으로확인되었다. 그러나조사결과작은빨간집모기 (Culex tritaeniorhychus), 흰줄숲모기 (Aedes albopictus), 중국얼룩날개모기 (Anopheles sinensis) 서식하는것으로확인되어향후모기에대한종합적인방제관리기준으로활용될수있다. Acknowledgements: This study was supported by fund (code: ) of the Korea Centers for Disease Control. Conflict of interest: None Author s information (Position): Seo MY, Professor; Chung KA, Professor. REFERENCES. Roh SJ, Lee WS. Global warming vs. Climate change : a framing effect of word choice in environmental communication. Korean Society for Journalism & Communication Studies. 203;9: Kim DM, Roh BY, Heo JH, Lee WG, Yang SC, Lee DK. Seasonal prevalence of mosquitoes collected from light traps in Gyeongsangnam province, Republic of Korea ( ). Entomological Research. 208;48: / Park SY, Han DK. Reviews in medical geography: spatial epidemiology of Vector-borne diseases. J Geographical Society. 202;47: Korea Centers for Diseasw Control. Guidelines for preparation management of main communication meters. st ed. cheongju: Korea Centers for Diseasw Control; 200. p Chung DH, Lee WG, Shin EH. Forecast of Culex tritaeniorhynchus as temperature change. J Korean Data Analysis Society. 205;7: Chae SM, Kim DJ, Yoon SJ, Shin HS. The impact of temperature rise and regional factors on malaria risk. Health and Social Welfare Review. 204;34: Chung MH. Dengue fever. Korean J Med. 2009;77: Fonseca DM, Keyghobadi N, Malcolm CA, Mehmet C, Schaffner F, Wilkerson RC. Emerging vector sin the Culex pipiens complex. Science. 2004;303: Jeong YS, Lee DK. Prevalence and seasonal abundance of the dominant mosquito species in a large marsh near coast of Ulsan. Korea J Entomol. 2003;42: World Meteorological Organization. WMO statement on the state of the global climate in 208. Geneva: World Meteorological Organization; 209. p6-2.. Kim YK, Lee CM, Lee JB, Bae YS. Seasonal prevalence of mosquitoes and ecological characteristics of Anopheline larval occurrence in Gimpo, Gyeonggi province, Republic of Korea. Korean J Appl Entomol. 202;5: Tanaka K, Mizusawa K, Saugstad ES. A revision of the adult and larval mosquitoes of Japan (including the Ryukyu Archipelago and the Ogasawara islands) and Korea (Diptera: Culicidae). American Entomological Institute. 979;6: Hong HK. Pictorial key to species of mosquitoes in Korea. Research report. Seoul: National Institute Health Korea; 982. p Lee CW, Hwang KK. Mosquito distribution and detection of flavivirus using real time RT-PCR in Jeju island 207. Korean J Appl Entomol. 208;57: KSAE Rhee SH, Kim TP, Lee TH, Kim MB, Yoon CH. Monitoring for prevalence of mosquitoes collected and pathogen infection. Korean J Vet Serv. 208;4: kjvs Chung SJ, Ko SH, Ko EM, Lim EJ, Kim YS, Lee WG. Mosquito prevalence and flavivirus infection rates in Gangwon-do, Republic of Korea. Korean J Appl Entomol. 209;58: Im SH, Jang JY. Correlations between climate change-related infectious diseases and meteorological factors in Korea. J Prev Med Public Health. 200;43: jpmph Lee DK. Occurrence of Culex pipiens (Diptera, Culicidae) and effect of vent net sets for mosquito control at septic tanks in south-eastern area. Korean J Appl Entomol. 2006;45:5-57.
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