(Establishment and Management of Proteomics Core Facility)

(Establishment and Management of Proteomics Core Facility)

1 sample running on SDS-PAGE Gel (10well) 163570 10 16357 1D size marker 225500 100 2255 Buffer(20X, 500ml) 119900 20 5995 Staining kit 299200 125 2393 27000

2-DE Gel construction for 1 sample 2D extraction buffer 138000 50 2760 13cm strip 191000 12 15916 Acry/Bis solution 114400 16 7150 Buffer 60714 3 20238 Staining kit 299200 12.5 23936 70000 MALDI-MS/MS analysis for 1 band(spot) in 1-D(2-D) gel after in-gel digestion Trypsin 135000 200 675 Tip 95 30 2850 Tube 80 3 240 Matrix standard kit 1197900 100 11979 Zip-tip 330000 96 3437 Source cleaning 1214400 365 3327 Plate cleaning kit 1199700 100 11997 AnchorChip 600/384 1900000 3840 495 35000 LC-MS/MS analysis for 1 sample in-gel digested Trypsin 135000 20 6750 Tip 95 30 2850 Tube 80 3 240 1D gel running 27000 Zip-tip 330000 96 3437 ACN 158400 40 3960 Water 33000 40 825 Analutic column 1518000 100 15180 Trap column 441800 100 4418 Spray tip 288200 10 28820 LC-MS Standard 652000 100 652 100000

- 국내주요단백질체연구기관의장비보유현황 (2007.9.) 1) KIST Molecular Proteomics lab. 장비 MALDI-TOF MALDI-TOF/TOF Triple quadruple Ion Trap Q-TOF FTICR 대수 1 1 0 2 0 0 2) 기초과학연구소 장비 MALDI-TOF MALDI-TOF/TOF Tripole quadrupole Ion Trap Q-TOF FTICR 대수 2 1 0 2 2 2 3) 삼성생명과학연구소 장비 MALDI-TOF MALDI-TOF/TOF Tripole quadrupole Ion Trap Q-TOF FTICR 대수 0 1 0 2 1 0 4) 연세프로테옴센터 장비 MALDI-TOF MALDI-TOF/TOF Tripole quadrupole Ion Trap Q-TOF FTICR 대수 0 1 0 1 2 0 5) 국립암센터 장비 MALDI-TOF MALDI-TOF/TOF Tripole quadrupole Ion Trap Q-TOF FTICR 대수 0 2 0 0 1 0 - 국내단백질체연구소장비보유현황조사에기반, 최신의 Ion Trap MS 도입 (2008.02.18) 그림 6. 새로도입된 LTQ-XL IonTrap MS

Sample Name y-17_1 Sample Name y-17_2 Reference P (pro) Score Scan(s) Peptide P (pep) XC Gene_Symbol=ERP29 Endoplasmic reticulum protein ERp29 precursor 4.35E-05 20.20 3550 K.GALPLDTVTFYK.V 9.33E-05 3.37 3553 K.GALPLDTVTFYK.V 4.35E-05 3.17 3742 K.SLNILTAFQK.K 2.15E-04 4.10 3765 K.SLNILTAFQK.K 2.06E-04 3.87 Reference P (pro) Score Scan(s) Peptide P (pep) XC Gene_Symbol=PHB Prohibitin 1.31E-05 20.20 3156 R.FDAGELITQR.E 2.11E-05 3.91 3170 R.FDAGELITQR.E 1.31E-05 3.88 3573 R.VLPSITTEILK.S 8.18E-05 2.03 3579 R.VLPSITTEILK.S 3.78E-04 2.78 Sample Name y-17_3 Reference P (pro) Score Scan(s) Peptide P (pep) XC Gene_Symbol=HSPB1 Heat shock protein beta-1 4.09E-06 10.21 3531 - R.LFDQAFGLPR.L 4.09E-06 4.30 3533

➅ Ast_01 Reference P (pro) Score 1 Gene_Symbol=ALB Uncharacterized protein ALB 1.17E-09 20.27 2 Gene_Symbol=PEPD Xaa-Pro dipeptidase 2.95E-08 10.14 3 Gene_Symbol=SERPING1 Plasma protease C1 inhibitor precursor 4.48E-07 10.14 4 Gene_Symbol=BGN Biglycan precursor 1.14E-06 10.16 5 6 Gene_Symbol=ACOT9 acyl-coenzyme A thioesterase 2, mitochondrial isoform b Gene_Symbol=DKFZp686D0972 hypothetical protein LOC345651 2.82E-06 6.15 3.14E-06 10.19 7 Gene_Symbol=LOC646821 similar to cytoplasmic beta-actin 3.14E-06 8.11 8 Gene_Symbol=SRRD Hemochromatosis protein (Fragment) 3.58E-06 2.16 9 Gene_Symbol=CFB Isoform 1 of Complement factor B precursor (Fragment) 6.99E-06 10.13 Ast_02 Reference P (pro) Score 1 Gene_Symbol=COL3A1 Isoform 1 of Collagen alpha-1(iii) chain precursor 4.53E-13 10.21 2 Gene_Symbol=ALB Uncharacterized protein ALB 2.35E-10 30.28 3 Gene_Symbol=BGN Biglycan precursor 5.57E-07 20.19 4 Gene_Symbol=MFSD5 Isoform 2 of Major facilitator superfamily domain-containing protein 5 4.84E-06 6.10

➆ Sample Name cox2_1 Sample Name cox2_2 Reference P (pro) Score Scan(s) Peptide P (pep) XC Gene_Symbol=PYGM Glycogen phosphorylase, 9.87E-06 10.15 muscle form 2416 R.VAAAFPGDVDR.L 9.87E-06 3.03 Reference P (pro) Score Scan(s) Peptide P (pep) XC Gene_Symbol=MYH9 Myosin-9 9.21E-12 376.27 2076 R.NAEQYKDQADKASTR.L 6.43E-05 3.51 2147 R.TEM*EDLM*SSKDDVGK.S 1.44E-08 3.18 2168 K.RQLEEAEEEAQR.A 4.92E-05 4.01 2206-2208 R.QLEEAEEEAQR.A 3.11E-06 3.30 2239 Q.LEEEEEAKHNLEK.Q 1.26E-06 3.58 2250-2252 R.ELEDATETADAM*NR.E 1.10E-07 4.68 2256 R.ALEEAM*EQKAELER.L 8.61E-08 3.07 2292 K.VKPLLQVSR.Q 3.80E-05 2.01 2301 K.VKLQEMEGTVK.S 4.30E-05 3.30 2311 R.TVGQLYKEQLAK.L 5.09E-07 4.59 2314 R.QLEEAEEEAQR.A 5.42E-06 3.52 2321 R.QEEEMM*AKEEELVK.V 2.94E-05 2.70 2324 R.QLEEAEEEAQR.A 3.05E-05 3.26 2326 T.LENERGELANEVK.V 6.51E-05 2.22 2329 K.ASITALEAK.I 1.38E-04 3.21 2332 K.YKASITALEAK.I 3.33E-07 3.29 2334 K.ASITALEAK.I 8.64E-05 3.14

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