Origlnal Articles Korean Circulation J 1998 ;288:1253-1259 관동맥질환에서저밀도지단백입자크기의분포 임효영 1 김인숙 2 승기배 1 강동헌 1 전두수 1 장기육 1 박준철 1 이수영 2 홍순조 1 최규보 1 Low-Density Lipoprotein Particle Size Distribution in Subjects with Coronary Artery Disease Hyo-Young Rhim, MD 1, In-Sook Kim, PhD 2, Ki-Bae Seung, MD 1, Dong-Hun Kang, MD 1, Ki-Yuc Jang, MD 1, Doo-Soo Jun, MD 1, Jun-Chul Park, MD 1, Soo-Young Lee, PhD 2, Soon-Jo Hong, MD 1 and Gyu-Bo Choi, MD 1 1 Department of Internal Medicine, 2 Chemistry, College of Medicine, Catholic University, Seoul, Korea ABSTRACT BackgroundLow-density lipoprotein LDL cholesterol has been shown to be a major risk factor for coronary artery disease CAD in animal studies, clinical trials, and observational epidemiologic studies. It has a hydrated density of 1.019 to 1.063 kg/l, a diameter of 20 to 30 nm, and displays -mobility on paper or agarose gel electrophoresis. With technique such as density gradient ultracentrifugation and gel electrophoresis, it is possible to separate lipoproteins accurately on the basis of their density, charge, and particle size. Further, it has been shown that a smaller LDL is associated with an increased risk of coronary artery disease, even when total cholesterol level is only slightly raised. The aim of this study was to analyze LDL particle size distribution in patients with angiographically confirmed coronary artery disease and in control subjects, using nondenaturating gradient polyacrylamide gel electrophoresis, and to investigate the relationship between LDL particle size and the other traditional coronary risk factors. MethodsBaseline characteristics such as age, sex, body mass index, history of hypertension or NIDDM, smoking habits, and plasma lipoprotein profiles were obtained in 33 and 27 subjects with and without CAD angiographically confirmed, respectively. We determined LDL peak particle diameter LDL-PPD using nondenaturating gradient polyacrylmide gel electrophoresis in CAD and control group. 4% to 12% polyacrylamide gradient gels were used for this assay, and the diameters of LDL subclass peaks were calculated by comparison with a standard calibration curve. This procedure permits the assignment according to distribution of particle diameters as exhibiting pattern A, B, or INT. ResultsTraditional coronary risk factorsage, sex, body mass index, history of hypertension or NIDDM, smoking habits, plasma triglyceride, HDL-cholesterol, and total to HDL-cholesterol ratio were found to be significantly different between two groups, except the plasma total cholesterol and LDLcholesterol. The mean value of LDL-PPD in patients with CAD wassignificantly lower than that in control subjects26.110.4 nm versus 27.011.9 nm, p0.006. LDL-PPD showed relatively strong associations with plasma triglyceride r0.536, p0.01), HDL-cholesterol r0.497, p0.01, and total to HDL-cholesterol ratio r0.516, p0.01, but showed no relation to total cholesterol r0.168 or LDL-cholesterol r 1253
0.028. ConclusionThese results suggest an association between small LDL and the presence of CAD and also suggest that LDL-PPD may be associated with the plasma lipid levels. Korean Circulation J 1998;288:1253-1259 KEY WORDSLow-density lipoprotein peak particle diameter LDL-PPD Coronary artery disease. 서론 환자 대상및방법 방법 1254 Korean Circulation J 1998;288:1253-1259
통계분석 결 관동맥질환위험인자의비교 Table 1. Baseline characteristics in subjects with or without coronary artery disease 과 CAD n33 Controls n27 p value Ageyears 6110 5212 0.005 Sex Male 2472.7% 933.3% 0.002 Female 927.3% 1866.7% BMIkg/m 2 25.23.3 23.03.2 0.011 NIDDM 1339.4% 414.8% 0.036 Hypertension 1648.5% 829.6% 0.138 Smoking 1545.5% 1 3.7% 0.001 1255
Table 2. Plasma lipid profiles in subjects with or without coronary artery disease CAD n33 Control n27 p value Total cholesterol mg/dl 197.528.5 190.633.8 0.405 Triglyceride mg/dl 166.398.2 115.176.5 0.027 HDL-cholesterol mg/dl 39.3 9.7 47.011.4 0.008 LDL-cholesterol mg/dl 124.930.2 120.630.0 0.579 T-chol/HDL-chol ratio 5.3 1.3 4.3 1.2 0.003 Table 3. Mean value of LDL-PPD and frequency of LDL subclass phenotype CAD n33 Control n27 p value LDL-PPDnm 26.110.4 27.011.9 0.006 LDL subclass 0.296 Pattern A 2678.8% 2488.9% Pattern B 721.2% 311.1% 혈청지질농도의비교 LDL-PPD분포의비교 LDL-PPD와혈청지질농도의관련성 Table 4. Person correlation coefficient between LDL- PPD and plasma lipid profiles Table 5. Classification of LDL particle by Musliner and Krauss 22) Class Subfraction density g/ml 고 안 LDL-PPD Particle diameter nm LDL I 1.0251.035 2627 LDL II 1.0321.038 25.526 LDL III A 1.0381.050 24.725.6 LDL III B 24.224.6 LDL IV A 1.0481.065 23.324.2 LDL IV B 21.823.2 p value Totalcholesterol 0.168 NS Triglyceride 0.536 0.01 HDL-cholesterol 0.497 0.01 LDL-cholesterol 0.028 NS Total-chol/HDL-chol ratio 0.516 0.01 1256 Korean Circulation J 1998;288:1253-1259
요약 연구배경 : 1257
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