Comparison of Total Flavonoids and Phenolic Contents of Propolis Collected in Korea Woo, Soon Ok*, In-Pyo Hong and Sangmi Han Sericultural & Apicultural Materials Division, Department of Agricultural Biology, National Institute of Agricultural Sciences, RDA, Wanju 5535, Republic of Korea (Received November 15; Revised 1 November 15; Accepted 1 November 15) Abstract Propolis is a sticky material like resin made from plant growing point which are collected by bees, then mixed with bee saliva enzyme. It is used to keep bee colony safe by applying inside of bee hive. The propolis, it is necessary to extract the active ingredient in raw material, mainly extracted by the ethyl alcohol. Ethanol extracted propolis collected from different regions in Korea, we compared the total flavonoids and total phenolic contents. The average of total flavonoids content of propolis were.1% in central regions, 5.7% in southern regions, however, propolis in Jeju island represented the average value of.%. The other areas showed a value of 7.%. The total phenolic contents of propolis were 1.% in the central region,.9% in southern regions, 13.7% in Jeju, and the other areas showed a value of 9.%. Although some differences depending on the region, the propolis produced in Korea has been as the substance that is rich in flavonoids and phenolics. Key words: Korean propolis, Total flavonoid contents,,,,, propolis (Ghisalberti, 1979).,,. pro, polis, *Corresponding author. E-mail: wooso1@korea.kr 93
9. (Ghisalberti, 1979; Greenaway et al., 1991; Marcucci, 1995; Bankova et al., ; Pietta et al., ; Kumazawa et al., ; Isla et al., 5; Silici and Kutluca, 5; Gomez-Caravaca et al., ; Mohammadzadeh et al., 7; Jasprica et al., 7; Alencar et al., 7).,, (Bors et al., 199; Heim et al., ; Russo et al., ; Kumazawa et al., ), (Burdock, 199; Markham et al., 199)., (Woo et al., 15).. 3, 9, 9, 9 7..,, %..1g 9% ml, (3,rpm, min). % ml 3 % 5ml..5ml 1.5ml, %.1ml, 1M.1ml,.ml. mm 15nm..1ml a(%). 5ml a(mg/ml) (g) 1,(mg) Folin-Ciocalteau (Kuyala et al., ),.5ml (% ethanol, 3 ) 1N Folin-Ciocalteau.5ml % Na CO 3.5ml 5 15g. 7nm UV/VIS Spectrophotometer(Perkin-Elmer Lambda ). (gallic acid) %(g/g gallic acid).
. (,,, ).~9.3%.1% (Fig. 1), 9.3%. (,,, ).~7.% 5.7% (Fig. ).., ~1.9%, 5 %. % (Fig. 3)..7~% (Fig. ). Kumazawa ().. Danyang Gwangmyung Goyang Hongchon Daejeon Uijeongbu Middle region of Korea Suwon Dangjin Yeoju Seoul Youngdong Jincheon Fig. 1. Total flavonoid contents of propolis collected in middle Jeju 1 Jeju Jeju 3 Jeju Jeju 5 Jeju Jeju 7 Jeju Jeju 9 Jeju island Fig. 3. Total flavonoid contents of propolis collected in Jeju island of Korea. Changnyoung Jinju Namwon Gurye Southern region of Korea Hwasun Jeonju Geochang Sacheon Andong Iksan Habcheon Fig.. Total flavonoid contents of propolis collected in southern Aldrich Foreig Origin Romania Polang Spain New Zealand Uruguay Chile Fig.. Total flavonoid contents of propolis collected in abroad. 95
9 (g/g gallic acid) 3 Danyang Gwangmyung Goyang Hongchon Daejeon Uijeongbu Middle region of Korea Suwon Dangjin Yeoju Seoul Youngdong Jincheon Fig. 5. Total phenolics contents of propolis collected in middle (g/g gallic acid) 3 Jeju 1 Jeju Jeju 3 Jeju Jeju 5 Jeju Jeju 7 Jeju Jeju 9 Jeju island Fig. 7. Total phenolics contents of propolis collected in Jeju island of Korea. (g/g gallic acid) 3 Changnyoung Jinju Namwon Gurye Southern region of Korea Hwasun Jeonju Geochang Sacheon Andong Iksan Habcheon Fig.. Total phenolics contents of propolis collected in southern 1~31% % (Fig. 5), 31%. 11~31% 1% (Fig. ),., ~% 1% (Fig. 7), 3%. 1~33% (Fig. ), 9%.,. Folin- Coicalteu. (g/g gallic acid) 3 Aldrich Fig.. Total phenolics contents of propolis collected in abroad..,,,.,. Foreign origin Romania Polang Spain New Zealand Uruguay Chile
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