[17].,,, [18,19].. Bakhsh [20], Sklan [21] Dawa [22], Kendler[18]. Makheja [23] Srivastava[24] thromboxane. Hughes [25], Dorsch [26]. Jurdi-Haldeman [
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1 임선영한국해양대학교해양환경생명과학부 1. (Allium cepa L.),,,, [1], [2-4], [5], [6], [7,8], [9], [10], [11].,,,, [12]. (polyphenol), C, E, - quercetin, quercitrin, rutin. diphenlypropane(c 6 -C 3 -C 6 ),,,, [13], Miean Mohamed[14] 62. hydroxylation, glycosylation,,,, 5,000 [15]..,,,,,. ally propyl disulfide dially disulfide quercetin, isorhamnetin, kaempferol, rutin [5,16]. 377
2 [17].,,, [18,19].. Bakhsh [20], Sklan [21] Dawa [22], Kendler[18]. Makheja [23] Srivastava[24] thromboxane. Hughes [25], Dorsch [26]. Jurdi-Haldeman [27].,. Woo [28],, Kim Lee[3],, LDL- HDL-,. 60g [29]. 50g., [30~32].,,, [33].,,, Kim [34] 378
3 , 13.29% 12.99% 10.78%. C, > >. [34,35].. [36,37]. 10% 10%. quercetin quercetin 0.01%, 6.5% [12]. 0.4~0.5%, 25~32% 50 [45] 재료및추출. (STVD-50, Sanya, Korea) 15~25, 15~40 mmhg
4 acetone methylene chloride 1 : rotary vacuum evaporator(n-1000, EYELA, Japan) aceton/methylene chloride (A+M). A+M methanol 2 methanol (MeOH). hexane, 85% aq. MeOH, butanol(buoh), water. dimethyl sulfoxide (DMSO). DMSO 0.05%. 2.2 일반성분및비타민 C 측정,,, AOAC [38]., (105 ), Kjeldahl, Soxhlet, 550. C HPLC(P580, Dionex, USA) [39]. 1g, 10% HPO 3 9ml 3,000rpm 15 20L HPLC C. column (u-bondapak C 18 ), 0.05M KH 2 PO 4 / acetonitrile(60 : 40) 1ml/min UV/VIS detector 254nm. L-ascorbic acid(sigma-adrich, USA). 2.3 총플라보노이드측정 1ml 10ml diethyleneglycol 1N NaOH 1ml 37 1 UV-visible spectrophotometer(helios Beta, Thermo Electron Corporation, USA) 420nm. rutin [38]. 2.4 총페놀측정 Folin-Denies [40]. 380
5 1mg 1ml 10 2ml 2 Folin 2ml 3 10% Na 2 CO 3 2ml 1. UV-visible spectrophotometer(helios Beta, Thermo Electron Corporation, USA) 750nm. tannic acid(sigma-aldrich, USA). 2.5 유리아미노산비교분석 10g 75% 100ml 80, M sodium citrate buffer(ph 2.2)(Merck, Germany) 5ml Sep-Pak C M membrane filter [41]. 2.6 색도측정 (CR-10, Konica Minolta Sensing, Inc., Japan), 3 total color difference(e) [42]. 2.7 암세포및암세포배양 () (HT-1080), (AGS) (HT-29). 100units/ml penicillin-streptomycin 10% FBS DMEM RPMI , 5% CO 2 incubator(mco-15ac, Sanyo Electric Biomedical Co., Japan). 2. phosphate buffered saline(pbs) 0.05% trypsin-0.02% EDTA(Gibco Co., USA) 75mm 3 cell culture flask 10ml 6~ MTT assay 96 well cell culture plate cells/ml 100L 37, 5% 381
6 CO 2 incubator 24 well 100L, PBS 100L. plate 37, 5% CO 2 incubator 24. MTT assay 3-(4,5-dimethylthiazol)-2,5- diphenyltetrazolium bromide(mtt) 5mg 1ml PBS, 10% FBS 9ml 100L 3~4 MTT. formazan well MTT. well formazan DMSO 100L 5~10 microplate reader(victor3, Perkin Elmer, USA) 540nm. MTT, well [43]. 2.9 세포내활성산소종 (reactive oxygen species) 측정 HT units/ml penicillin-streptomycin 10% FBS DMEM cell culture dish 37, 5% CO 2 incubator. DCFH-DA(dichlorodihydrofluorescin diacetate) assay [44]. DCFH-DA(Sigma- Aldrich, USA) (dichlorofluorescein, DCF). 96 well cell culture plate 24, PBS 20M DCFH-DA well 37, 5% CO 2 incubator 20 pre-incubation. well 37, 5% CO 2 incubator 1, DCFH-DA PBS 500M H 2 O 2 DCF fluorescence excitation 488nm, emission 530nm microplate reader(victor3, Perkin Elmer, USA). (blank control) PBS, control 500M H 2 O 2, blank 500M H 2 O 2 PBS. 382
7 저온진공건조된양파분말의화학성분및생리활성 C C % 7.15%. C, 6.27mg/100g 17.34mg/100g 9.55mg/100g. 1 Chemical composition of onion powder as affected by drying methods Components Raw Vacuum drying Freeze drying Moisture (%) Carbohydrate (%) Crude protein (%) Crude fat (%) Crude ash (%) Vitamin C (mg/100 g) phosphoethanolamine, L-aspartic acid, L-glutamic acid, L-alanine, L-citrulline, -aminobutryric acid. phosphoserine, phosphoethanolamine, L-valine, L-isoleucine. 383
8 2 Free amino acid contents of onion powder by different drying methods (ppm in sample) Amino acids Raw Vacuum drying Freeze drying Phosphoserine Taurine Phosphoethanolamine , ,588.3 Urea L-Aspartic acid ,060.3 L-Threonine L-Serine Asparagine L-Glutamic acid ,578.5 L--Aminoadipic acid L-Proline L-Glycine L-Alanine , ,003.8 L-Citrulline , L--Aminobutric acid L-Valine , L-Cystine L-Methionine L-Isoleucine L-Leucine L-Tyrosine L-Phenylalanine Alanine Aminobutyric acid Aminobutyric acid 2, , ,412.0 NH L-Orinithine L-Lysine Mothyl-L-Histidine L-Histidine Methyl-L-Histidine L-Carnosine L-Arginine Total 4, , ,
9 3. Hunter-L > >. Hunter-a Hunter-b > >. 3 Color of onion powder as affected by drying methods Color Raw Vacuum drying Freeze drying L-value a-value b-value , C 4,. 93.1% 14.3% %, %. C, 6.27mg/100g g/ml. 4 Chemical composition of onion flesh and peel Components Onion flesh Onion peel Moisture (%) Carbohydrate (%) Crude protein (%) Crude fat (%) Crude ash (%) Vitamin C (mg/100 g) Total flavonoids (g/ml) A+M MeOH 0.05mg/ml HT M H 2 O 2 control 385
10 120, A+M ( 1). 2 hexane, 85% aq. MeOH, butanol(buoh), water 0.05mg/ml control. ( 2A), 85% aq. MeOH BuOH hexane water control. ( 2B) 85% aq. MeOH hexane water contorl. A+M 85% aq MeOH BuOH. Ra [5] butanol> ethyl acetate> ethyl ether> water. Kim Kim[45], 100,, 1 Inhibitory effect of acetone/methylene chloride (A+M, 0.05mg/ml) and methanol (MeOH, 0.05mg/ml) extracts of onion flesh and peel on production of cellular reactive oxygen species in HT-1080 cells. Control was treated with 500M H 2 O 2 and phosphate buffered saline. Blank was treated with phosphate buffered saline without H 2 O
11 2 Inhibitory effect of solvent fractions (0.05mg/ml, A; Onion peel: B; Onion flesh) of extracts from onion flesh and peel on production of cellular reactive oxygen species in HT-1080 cells. Control was treated with 500M H 2 O 2 and phosphate buffered saline. Blank was treated with phosphate buffered saline without H 2 O 2..,, 387
12 . LDL thiobarbituric acid reactive substances(tbars) LDL TBARS scavanger quercetin LDL. Park Kim[46] 5% TBARS quercitin. Shon [47] -carotene-linoleate system BHT L-ascorbic acid. MTT assay. 3 acetone/methylene chloride (A+M) methanol (MeOH) 0.1, 0.5 1mg/ml AGS. A+M 0.1mg/ml 3 Inhibitory effect of acetone/methylene chloride (A+M) and methanol (MeOH) extracts from onion flesh and peel on the growth of AGS human gastric adenocarcinoma cells. *p<0.05, significantly different from control. #p<0.05, significant effect between onion flesh and peel. 388
13 AGS 0.5mg/ml 95%, 1mg/ml 96% (p<0.05). A+M A+M 1mg/ml 13%. MeOH A+M 1mg/ml 23 35%. 4 hexane, 85% aq. MeOH, BuOH, water 4 Inhibitory effect of solvent fractions of extracts from onion flesh and peel on the growth of AGS human gastric adenocarcinoma cells. *p<0.05, significantly different from control. #p<0.05, significant effect between onion flesh and peel. 389
14 5 Inhibitory effect of acetone/methylene chloride (A+M) and methanol (MeOH) extracts from onion flesh and peel on the growth of HT-29 human colon cancer cells. * p<0.05, significantly different from control. # p<0.05, significant effect between onion flesh and peel. 85% aq. MeOH 0.5mg/ml 90% (p<0.05). HT-29 ( 5), AGS A+M 0.5 1mg/ml 90% MeOH AGS 1mg/ml 44%. A+M MeOH 1mg/ml 28 21%. 6 hexane, 85% aq. MeOH, BuOH, water HT-29 85% aq. MeOH (p<0.05). 85% aq. MeOH 0.5mg/ml 93% BuOH 46 42% (p<0.05). 5 IC 50 A+M IC 50 AGS HT mg/ml MeOH IC mg/ml AGS. 85% aq. MeOH AGS IC mg/ml 390
15 . HT-29 AGS. water. A+M MeOH. Lee [19] Inhibitory effect of solvent fractions of extracts from onion flesh and peel on the growth of HT-29 human colon cancer cells. *p<0.05, significantly different from control. #p<0.05, significant effect between onion flesh and peel. 391
16 25 50mg %, quercitin, detoxification enzyme. Dorant [48] allicin, Sankaranrayanan [49] case-control. AGS. 85% aq. MeOH. 5 IC50 values of onion flesh and peel extracts on human cancer cell lines IC 50 Concentrations (mg/ml) AGS HT-29 A+M extract Flesh Peel MeOH extract Flesh Peel Hexane fraction Flesh Peel % aq. MeOH fraction Flesh Peel BuOH fraction Flesh Peel Water fraction Flesh Peel
17 3.1 건조붉은양파의화학성분및생리활성, C 17.95% 80% ( 6).,, 62.96, 8.74, %. C, 15mg/100g 96mg/100g. (34.29mg/ml) 39.05mg/ml. 6 Chemical composition of red onion Flesh red onion Dried red onion Moisture (%) ) Carbohydrate (%) Crude protein (%) Crude fat (%) Crude ash (%) Vitamin C (mg/100 g) Total phenol (mg/ml) ) Values are Mean S.E. 7 L-aspartic acid, L-glutamic acid, L-alanine, L-leucine, -aminobutryric acid. phosphoserine, phosphoethanolamine, L-aspartic acid, L-alarine, L-citrulline, L-valine, L-leucine, L-phenylalanine, -aminobutryric acid, L-histidine. 393
18 7 Free amino acid contents of red onion (ppm in sample) Amino acids Raw Vacuum drying Phosphoserine Taurine - - Phosphoethanolamine ,343.2 Urea - - L-Aspartic acid L-Threonine L-Serine Asparagine - - L-Glutamic acid L--Aminoadipic acid - - L-Proline - - L-Glycine L-Alanine ,184.7 L-Citrulline L--Aminobutric acid - - L-Valine L-Cystine - - L-Methionine - - L-Isoleucine L-Leucine L-Tyrosine L-Phenylalanine Alanine - - -Aminobutyric acid - - -Aminobutyric acid 3, ,479.9 NH L-Orinithine - - L-Lysine Mothyl-L-Histidine - - L-Histidine Methyl-L-Histidine - - L-Carnosine - - L-Arginine - - Total 4, ,
19 8. Hunter-L Hunter-b. Hunter-a Color of red onion powder Color Raw Vacuum drying L-value a-value b-value acetone/methylene chloride (A+M) methanol (MeOH) 0.1 5mg/ml HT A+M 0.25mg/ml HT mg/ml 96%, 5mg/ml 98% 9 Inhibitory effect of acetone/methylene chloride (A+M) and methanol (MeOH) extracts of red onion powder on the growth of HT-1080 human fibrosarcoma cells Treatments A+M extract MeOH extract *p<0.05, significantly different from control. Concentration Inhibition IC 50 (mg/ml) (%) (mg/ml) * * * 5 98* * 5 50* 10 89*
20 (p<0.05). MeOH, MeOH 5 10mg/ml 50 89% (p<0.05). A+M MeOH IC mg/ml A+M MeOH. hexane, 85% aq. MeOH, butanol(buoh), water HT-1080 ( 10), 5mg/ml water 86% 10 Inhibitory effect of solvent fractions of extract from red onion powder on the growth of HT-1080 human fibrosarcoma cells Treatment Hexane fraction 85% aq. MeOH fraction BuOH fraction Water fraction *p<0.05, significantly different from control. Concentration Inhibition IC 50 (mg/ml) (%) (mg/ml) * * * * 5 93* 10 98* * 5 86* 10 98* * 5 94* 10 97* * * * 5 43* 10 86*
21 (p<0.05), hexane IC mg/ml. 11 HT-29, HT-1080 A+M MeOH HT mg/ml A+M 91 97% (p<0.05), IC mg/ml. MeOH 5 10mg/ml 58, 70% IC mg/ ml HT-29 A+M (p<0.05). 12 hexane, 85% aq. MeOH, BuOH, water HT-1080 water 5mg/ml 88%. hexane 85% aq. MeOH IC mg/ml. HT-1080 HT-29 A+M MeOH. water HT-1080 HT-29. Rho Han[6] NCI-H522 NCI-H596 NCI-H522 48%, NCI-H596 25% NCI-H596, Lee 11 Inhibitory effect of acetone/methylene chloride (A+M) and methanol (MeOH) extracts of red onion powder on the growth of HT-29 human colon cancer cells Treatments A+M extract MeOH extract *p<0.05, significantly different from control. Concentration Inhibition IC 50 (mg/ml) (%) (mg/ml) * * * * 5 97* * * * * 5 58* 10 70*
22 [19] 20mg 55%, 25 50mg 32 34%. 12 Inhibitory effect of solvent fractions of extract from red onion powder on the growth of HT-29 human colon cancer cells Treatments Hexane fraction 85% aq. MeOH fraction BuOH fraction Water fraction *p<0.05, significantly different from control. Concentration Inhibition IC 50 (mg/ml) (%) (mg/ml) * * * * 5 98* 10 99* * * * * 5 88* 10 87* * * * * 5 95* 10 94* * * * * 5 52* 10 84*
23 A+M MeOH mg/ml HT A+M MeOH 500M H 2 O 2 control 120. hexane, 85% aq. MeOH, BuOH, water 8 HT mg/ ml 85% aq. MeOH. water. A+M, MeOH. Shon Park[50],. Shahidi Wanasundara[51] phenolic hydroxyl groups. Shon [47], 2. Jeong [52] C mg/100g C C. C. Kim [53] -carotene-linoleate system butylated hydroxy toluene(bht) L-ascorbic acid. 20~30%, FRAP(Ferric reducing ability of plasma), [54]. in vitro in vivo thiobarbituric acid reactive 399
24 7 Inhibitory effect of extracts of red onion powder on production of reactive oxygen species in HT-1080 cells. 1) Control was treated with 500M H 2 O 2 and phosphate buffered saline. 2) Blank was treated with phosphate buffered saline without H 2 O 2. 3) A+M 0.01, acetone with methylene chloride extract 0.01mg/ml; MeOH 0.01, methanol extract 0.01mg/ml (A). 4) A+M 0.05, acetone with methylene chloride extract 0.05mg/ml; MeOH 0.05, methanol extract 0.05mg/ml (B). 400
25 8 Inhibitory effect of solvent fractions of red onion powder on production of cellular reactive oxygen species in HT-1080 cells. 1) Control was treated with 500M H 2 O 2 and phosphate buffered saline. 2) Blank was treated with phosphate buffered saline without H 2 O 2. 3) Hexane 0.01, hexane fraction 0.01mg/ml; 85% aq. MeOH 0.01, 85% aqueous methanol fraction 0.01mg/ml; BuOH 0.01, butanol fraction 0.01mg/ml; water 0.01, water fraction 0.01mg/ml (A). 4) Hexane 0.05, hexane fraction 0.05mg/ml; 85% aq. MeOH 0.05, 85% aqueous methanol fraction 0.05mg/ml; BuOH 0.05, butanol fraction 0.05mg/ml; water 0.05, water fraction 0.05mg/ml (B). 401
26 substances(tbars) [55] C. 48. A+M MeOH 0.05mg/ml (HT-1080). 85% aq. MeOH BuOH 85% aq. MeOH. A+M MeOH, (HT-29) (AGS). 85% aq. MeOH AGS IC mg/ml. 80% C. HT-1080 HT-29 A+M MeOH, hexane IC mg/ml HT A+M, MeOH, hexane, 85% aq. MeOH, BuOH, water 500M H 2 O 2 control, 120. hexane 402
27 85% aq. MeOH. 5%,.,,,,,. 1) Block, E Antithrombotic organosulfur compounds from garlic. J. Am. Soc. 108, ) Kim, K. M., H. J. Suh, S. H. Chung, W. D. Cho and S. J. Ma Chemical structure of angiotensin converting enzyme inhibitor isolated from onion flesh. Food Sci. Biotechnol. 8, ) Kim, S. O. and M. Y. Lee Effect of ethylacetate fraction of onion on lipid metabolism in high cholesterol fed rats. J. Kor. Soc. Food Sci. Nutr. 30, ) Ma, S. J Inhibitory effect of onion seasoning on angiotensin converting enzyme. J. Kor. Soc. Food Sci. Nutr. 29, ) Ra, K. S., H. J. Suh, S. H. Chung and J. Y. Son Antioxidant activity of solvent extract from onion skin. Kor. J. Food Sci. Technol. 29, ) Rho, S. N. and J. H. Han Cyotoxicity of garlic and onion methanol extract on human lung cancer cell lines. J. Kor. Soc. Food Sci. Nutr. 29, ) Lee, S. H., S. J. Woo, Y. J. Koo and H. K. Shin Effects of mugwort, onion and polygalate radix on the intestinal environment of rats. Kor. J. Food Sci. Technol. 27, ) Rao, R. R., S. S. Rao and P. R. Venkataraman Investigations on plant antibiotics, studies on allicin, the antibacterial principles of Allium sativum L(Garlic). J. Sci. Ind. Res. (India). 18, ) Sheo, H. J., H. J. Lim and D. L. Jung Effect of onion juice on toxicity of lead in rat. J. Kor Soc. Food Sci. Nutr. 22, ) Park, P. S., B. R. Lee and M. Y. Lee Effects of onion diet on carbon tetrachloride toxicity of rats. J. Kor. Food Nutr. 20, ) Park, P. S., B. R. Lee and M. Y. Lee Effects of onion juice on ethanol-induced hepatic lipid peroxidation in rats. J. Kor. Food Nutr. 23, ) Cha, J. Y. and J. Y. Han The antioxidant ability of grape seed extracts. Kor. J. Food Sci. Technol. 34, ) Bravo. L Polyphenols: chemistry, dietary sources, metabolism and nutritional significance. Nutr. Reviews. 51, ) Miean, K. H. and S. Mohamed Flavonoid (myricetin, quercetin, kaempferol, luteolin, and apigenin) content of edible tropical plant. J. Agric. Food Chem. 49, ) Freucenbeim, J. L., J. R. Marshall, J. E. Vena, R. Laughlin, J. R. Brasure, M. K. Swanson, T. Nemoto and S. 403
28 Graham Premenopausal breast cancer risk and intake of vegetables, fruits and realted nutrients. J. Natl. Cancer Inst. 88, ) Bilyk, A., P. I. Cooper and G. M. Sapers Varietal differences in distribution of quercetin and kaempferol in onion (Allium cepa L.) tissue. J. Agric. Food Chem. 32, ) Rhim, J. S Onion and health, International Culture Publishing Co., Korea. pp ) Kendler, B. S Garlic (Allium sativum) and onion (Allium cepa): a review of their relationship to cardiovascular disease. Prev. Med. 16, ) Lee, C. J., H. D. Kim, E. H. Choung, J. K. Suh, C. W. Park and J. K. Ha Reduction effect of carcinogen induced mouse epidermal and forestomach carcinogenesis by the extract of oinon wastes. J. Kor. Soc. Food Sci. Nutr. 29, ) Bakhsh, R. and S. Khan Influence of onion (Allium cepa) and chaunga (Caraluma tubercula) on serum cholesterol, triglycerides, total lipids in human subject. Sarhad J. Agric. 6, ) Sklan, D., Y. N. Bermer and H. Rabinowitch The effect of dietary onion and garlic on hepatic lipid concentrations and activity of antioxidative enzymes in chicks. J. Nutr. Biochem. 3, ) Dawa, F. and P. K. Joseph Toxic effects of excess onion extract in rats. J. Food Sci. Technol. 26, ) Makheja, A. N., J. Y. Vanderhoek and J. M. Bailley Effects of onion (Allium cepa) extract on platelet aggregation and thromboxane synthesis. Prostaglandins and Med. USA. 2, ) Srivastava, K. C Onion exerts antiaggregatory effects by altering arachidonic acid metabolism in platelets. Prostaglandins Leukot. Med. 24, ) Hughes, B. G. and L. D. Lawson Antimicrobial effects of Allium sativum L., Allium ampeloprasum L. and Allium cepa L. Phytother. Res. USA. 5, ) Dorsch, W. and H. Wagner New antiasthmatic drugs of plant origin. Immunol. 14, ) Jurdi-Haldeman, D., J. H. MacNeil and D. M. Yared Antioxidant activity of onion and garlic juices in stored cooked ground lamb. J. Food Prot. 50, ) Woo, H. S., B. J. An, J. H. Bae, S. Kim, H. J. Choi, H. S. Han and C. Choi Effect of biologically active fractions from onion on physiological activity and lipid metabolism. J. Kor Soc. Food Sci. Nutr. 32, ) FIT Research Center and Mokpo National University International Symposium on the Utilization and Processing of Onions. p ) Chung, H. D Control of onion bulb rot during storage at low temperature by postharvest treatment of fungicides. J. Korean Soc. Hort. Sci. 23, ) Kim, H. K., H. C. Lee, M. H. Park and D. H. Shin Microflora of decayed onion bulbs and their suppression by fumigation treatment. Kor. J. Food Sci. Technol. 18, ) Lee, H. C., H. K. Kim, M. H. Park and D. H. Shin Confirmation of saprophytes of onions in Korea and effects of temperature, humidity and fumigation on Boyrtis-rot. Kor. J. Appl. Microbiol. Bioeng. 12, ) Da-Mota, V. M. and E. Palau Acoustic drying of onion. Drying Technol. 17, ) Kim, H. R., E. J. Seog, J. H. Lee and J. W. Rhim Physicochemical properties of onion powder as influenced by drying methods. J. Kor. Soc. Food Sci. Nutr. 36, ) Kang, N. S., J. H. Kim and J. K. Kim Modification of quality characteristics of onion powder by hot-air, vacuum and freeze drying methods. Kor. J. Food Preserv. 18, ) Kim, K. K Thermal characteristics of agriculture and fisheries by low temperature vacuum dryer. Proceedings of the KSME 1999 Spring Annual Meeting,
29 37) Kim, K. K., B. Y. Sung, H. S. Jung, S. Y. Choi and S. B. Moon A study on the thermal characteristics of the large low temperature vacuum dryer for biological drying. J. Kor. Soc. Marine Engineers. 24, ) AOAC International Official methods of anlysis of AOAC International 18th ed. AOAC International. 39) Vallls, F., M. T. Sancho, M. A. Ferrandez-Muino, S. Alonso-Torre and M. A. Checa High-pressure liquid chromatographic determination of ascorbic acid in cooked sausages. J. Food Prot. 65, ) Amerinem, M. A. and C. S. Ough Method for analysis of Musts and Win. Wiley & Sons. New York, USA. pp ) Lee, M. K. and H. Park Free amino acids of xylem-pith in panax ginseng root. Kor. J. Ginseng Sci. 11, ) Lee, K. S., H. P. Kwan, H. L. Sang, E. O. Choe and H. G. Lee The quality properties of dried carrots as affected by blanching and drying methods during storage. Kor. J. Food Sci. Technol. 35, ) Denizot, F. and R. Lang Rapid colorimetric assay for cell growth and survival modifications to the tetrazolium dye procedure giving improved sensitivity and reliablity. J. Immunol. Methods 89, ) LeBel, C. P., H. Ischiropoulos and S. C. Bondy Evaluation of the probe 2, 7-dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress. Chem. Res. Toxicol. 5, ) Kim, S. K. and M. K. Kim Effect of dried powders or ethanol extracts of onion flesh and peel on lipid metabolism, antioxidative and antithrombogenic capacities in 16-month-old rats. Kor. J. Nutr. 37, ) Park, J. and M. K. Kim Effects of onion flesh or peel feeding on antioxidantive capacity in 16-month-old rats high iron diet. Kor. J. Food Culture 20, ) Shon, M. Y., S. D. Choi, G. G. Kahng, S. H. Nam and N. J. Sung Antimutagenic, antioxidant and free radical scavenging activity of ethyl acetate extracts from white, yellow and red onions. Food Chem. Toxicol. 42, ) Dorant, E., P. A. van den Brandt and R. A. Goldbohm A prospective cohort study on Allium vegetable consumption, Netherlands. Cancer Res. 54, ) Sankaranrayanan, R., C. Varghese, S. W. Duffy, G. Padmakumary and N. E. Day. A case-control study of diet and lung cancer in Keraka, South India. Int. J. Cancer 75, ) Shon, M. Y. and S. K. Park Chemical components and nitrite scavenging activity of various solvent extracts from onions. Kor. J. Food Preserv. 13, ) Shahidi, F. and P. K. Wanasundara Phenolic antioxidants. Critical Rev. Food Sci. Nutr. 32, ) Jeong, C. H., J. H. Kim and K. H. Shim Chemical components of yellow and red onion. J. Kor. Soc. Food Sci. Nutr. 35, ) Kim, Y. H., M. Y. Shon and N. J. Sung Antioxidant and antimutagenic activities of hot water extract from white and yellow onions after simulated gastric digestion. J. Lif e Sci. 14, ),,, Proceedings of the Korean Nutrition Society Conference. Seoul. p ) Park, J., J. Kim and M. K. Kim Onion flesh and onion peel enhance antioxidant status in aged rats. J. Nutr. Sci. Vitaminol. 53,
제 출 문 경상북도 경산시 농업기술센터 귀하 본 보고서를 6차산업수익모델시범사업 농산물가공품개발 연구용역 과제의 최종보고서로 제출합니다. 2014 년 11 월 19 일 주관연구기관명 : 영남대학교 총괄연구책임자 : 한 기 동 연 구 원 : 김 상 욱 이 수 형 이 상
6차 산업 수익모델 시범사업 농산물가공품개발 연구용역 최 종 보 고 서 영남대학교 식품공학과 연구책임자 : 한 기 동 제 출 문 경상북도 경산시 농업기술센터 귀하 본 보고서를 6차산업수익모델시범사업 농산물가공품개발 연구용역 과제의 최종보고서로 제출합니다. 2014 년 11 월 19 일 주관연구기관명 : 영남대학교 총괄연구책임자 : 한 기 동 연 구 원 : 김
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