786 심길보ㆍ김민아ㆍ윤나영ㆍ송미영ㆍ정수정ㆍ임치원 (Ha and Kang, 990),.,,,,,,. 재료 재료및방법, , Table. 일반성분 AOAC (995), Kjeldahl, Soxhlet,. 지방산조성 Bligh and Dyer (959) 5 chl

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1 한수지 49(6), , 206 Original Article Korean J Fish Aquat Sci 49(6),785-79,206 쏘가리 (Siniperca scherzeri) 의시기에따른영양성분변화 심길보 * 김민아 윤나영 송미영 정수정 임치원 국립수산과학원식품위생가공과, 국립수산과학원중앙내수면연구소 Seasonal Variation in the Nutritional Composition of the Muscle of Mandarin Fish Siniperca scherzeri Kil Bo Shim*, Min A Kim, Na Young Yoon, Mi Young Song, Su Jung Jung and Chi Woon Lim Food Safety and Processing Research Division, National Institute of Fisheries Science, Busan 46083, Korea Central Inland Fisheries Research Institute, National Institute of Fisheries Science, Gapyeong 2453, Korea Seasonal variation in the composition and fatty acid, amino acid, cholesterol, and vitamin contents in the muscles of the mandarin fish Siniperca scherzeri, caught in Soyangho Lake, Korea, was studied. The moisture content was g/00 g, lipids ranged between.06 and 4.23 g/00 g, protein between g/00 g, and ash between 0.74 and 2.04 g/00 g. The moisture content was lowest in May and tended to increase from June to October. The highest protein levels occurred in May, which coincided with the timing of oocyte maturation, and then decreased at the beginning of spawning. The highest lipid levels occurred in May and November coinciding with the periods before spawning and hibernation. The ratio of EAA/NEAA was higher than the value recommended by FAO for an ideal food protein. There were significant differences in Mg and Fe contents with spawning; they were highest in May. Contents of vitamin A and E were from not detected (ND) to 7. µg/00 g and ND to 0.69 mg/00 g, respectively and were highest in November. The content of niacin tended to decrease in June and increased until November and hibernation. The cholesterol content was mg/00 g; it decreased from March to July and then increased until November. In conclusion, there is potential for expanding the commercial utilization of the mandarin fish as a food resource. Key words: Mandarin fish, Seasonal variation, Proximate composition, Niacin, Hibernation 서론 (Siniperca scherzeri) (Order Perciformes),,.,,, (Lee et al., 2005; Kim and Kang,993;Lee et al.,997)., 5-7, 5, 6 (Baek et al., 205; Lee et al., 2002)., (Baek et al., 205) ,056, 26,237., 04 (KOSIS, 206).,,,,, (Gye et al., 205; Oh et al., 2008; Yang and Lee, 983; Choi et al., 984; Kim and Lee, 986; Park and Kim, 996; Kang et al., 992), Korean J Fish Aquat Sci 49(6) , December 206 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Licens ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Received 28 October 206; Revised 22 November 206; Accepted 29 November 206 *Corresponding author: Tel: Fax: address: kilbo22@korea.kr Copyright 206 The Korean Society of Fisheries and Aquatic Science 785 pissn:0374-8, eissn:

2 786 심길보ㆍ김민아ㆍ윤나영ㆍ송미영ㆍ정수정ㆍ임치원 (Ha and Kang, 990),.,,,,,,. 재료 재료및방법, , Table. 일반성분 AOAC (995), Kjeldahl, Soxhlet,. 지방산조성 Bligh and Dyer (959) 5 chloroform:methanol (2:, v/v), 4% BF 3 -methanol methyl ester, gas chromatography (GC; Shimazu GC 200 Plus, Shimazu, Tokyo, Japan). 총아미노산함량 200 mg 6 N HCl 24 0., ph ml 0.20 m membrane filter,. (Sykam DE/S-433D, Sykam, Eresing, Germany). 미네랄함량 g 65% (Suprapur grade, Merck, Darmstadt, Gremany) 0 ml ml. 2% (ADVANTEC No.5C, Tokyo, Japan) 00 ml Inductively coupled plasma spectrometry (ICP; Optima 3300XL, PerkinElmer, Waltham, MA, USA). (MFDS, 204). 콜레스테롤함량 2-3 g 95% ethanol 40 ml 8 ml 50% KOH % ethanol 60 ml toluen 00 ml ml M KOH 0 ml M KOH 40 ml 0. toluene 40 ml toluene. toluene 20 g ml. 3 ml dimethylformamide ml 0.2 ml hexamethyldisilane 0. ml trimethylchlorosilane 5 -cholestane (MFDS, 205). HP- (30 m 0.25 mm 0.25 m, Agilent, USA) gas chromatography (GC; Agilent 7890, Agilent, CA, USA) (MFDS, 205). 비타민 A, E 함량 A, E Kwak et al. (2004). 3 g ethanol 30 ml, 60% KOH 3 ml, 0% pyrogallol ml 30, 30 ml. petroleum ether, petroleum ether 50 ml 2. petroleum ether 0 ml phenolphthalein, petroleum ether sodium sulfate. Petroleum ether 25 C methanol 5 ml 0.25 m membrane filter. Develosil RP-Aqueous C30 ( mm, 5 m particle size, Phenomenex, Tokyo, Japan) HPLC (Surveyor Plus HPLC system, Thermo, CA, USA).

3 쏘가리의계절별영양성분변화 787 Niacin 함량 5-0 g 5 mm Sodium hexanesulfonate 50 ml rpm 30, (4, 3,699 g 5 ) 0.2 µm membrane syringe filter (MFDS, 205). C8 column ( mm 5 m, Waters, USA) A: 5 mm hexanesulfonate/0.% acetic acid, B: 35% 5 mm hexanesulfonate/0.% acetic acid 65% MeOH,.0 ml/min, 0 L 260 nm HPLC (Surveyor Plus HPLC system, Thermo, CA, USA). 통계처리, Duncan s multiple range test (P<0.05) (Steel and Torrie, 980). 결과및고찰 가식부수율및일반성분변화 %, 3 6 (Table ). Table g/00 g, g/00 g (P>0.05) g/00 g, g/00 g, ,.06 g/00 g g/00 g g/00 g, g/00 g, g/00 g g/00 g.,, (Park et al., 995)., (Park et al., 995)., 5,,. (Siniperca chuatisi, Siniperca scherzeri) Table. The capture date and biological data of mandarin fish Siniperca scherzeri Month Total length (mm) Total weight (g) Yield (%) Mar ± ± ±9.07 ab) Apr. 287.± ± ±5.33 abc May 237.0± ± ±.42 abc Jun ± ± ±8.0 c Jul ± ± ±3.8 ab Aug ± ± ±6.8 a Sep ± ± ±.59 bc Oct. 25.7± ± ±7.3 ab Nov ± ± ±2.50 bc Different superscripts within a same column are significantly different by Duncan's multiple range at P<0.05. Table 2. Seasonal variation in proximate composition content (g/00 g) of the muscle of mandarin fish Siniperca scherzeri Month Moisture Crude lipid, 2 g/00 g, g/00 g (Chu et al., 203), 79.7 g/00 g, 0.9 g/00 g, 0.8 g/00 g, 5.7 g/00 g (Ha and Kang, 990)., 5. 지방산조성비변화 Crude protein Ash Mar. 77.9±0.49 a2 2.23±0.09 d 9.±0.25 c 2.04±0.32 a Apr. 77.±0.36 ab 2.99 c 8.5±0.6 d.38±0.03 b May 74.5±0.6 b 3.46±0.26 b 20.7±0.38 a 0.98±0.05 cd Jun. 78.0±0.5 a.69±0.06 e 9.7±0. b.05±0.06 c Jul. 78.7±0. a.06±0.09 f 9.8±0.2 b.52±0.2 b Aug. 76.9±3.94 ab.2±0.9 f 9.2±0.24 c 0.74±0.05 d Sep. 78.6±0.38 a.94±0.09 e 8.9±0.8 cd 0.99±0.05 dc Oct. 76.6±0.4 ab.92±0.6 e 20.7±0.45 a.23±0.2 bc Nov. 75.0±0.4 b 4.23±0.09 a 9.9±0.32 b.06±0.22 c Mean±standard deviation. 2 Different superscripts within a same column are significantly different by Duncan's multiple range at P<0.05. Table 3., palmitic acid (C6:0), oleic acid (C8:), linoleic acid (C8:2n6), docosahexaenoic acid (C22:6n-3) eicosa-

4 788 심길보ㆍ김민아ㆍ윤나영ㆍ송미영ㆍ정수정ㆍ임치원 Table 3. Seasonal variation in fatty acid composition (%) of the muscle of mandarin fish Siniperca scherzeri Fatty acid Mar. Apr. May Jun. Jul. Aug. Sep. Oct. Nov. C4: C6: C8: Saturated C6: C8: C20: Monoen C8:2n C8:3n C8:4n C20:4n C20:5n-3(EPA) C22:6n-3DHA) Polyen pentaenoic acid (C20:5n-3) %, % %, % EPA % % 3.28%. DHA % %, 7.75%. C8: %, 7.8%. 총아미노산함량변화 g/00 g, leucine, lysine, aspartic acid, glutamic acid, alanine, arginine g/00 g, g/00 g (Table 4) g/00 g, g/00 g g/00 g, , FAO (FAO, 957) , (Chu et al., 203). 미네랄함량변화 Table 5.,,, mg/00 g, mg/00 g, mg/00 g mg/00 g, mg/00 g (Oh et al., 980), 263 mg/00 g, 400 mg/00 g (RDA, 20), mg/00 g, mg/00 g (Gye et al., 205),, 7,, mg/00 g mg/00 g, mg/00 g mg/00 g, mg/00 g, 200. mg/00 g mg/00 g mg/00 g

5 쏘가리의계절별영양성분변화 789 Table 4. Seasonal variation in amino acid content (g/00 g) of the muscle of mandarin fish Siniperca scherzeri Amino acids Mar. Apr. May Jun. Jul. Aug. Sep. Oct. Nov. Essential amino acids (E) Histidine Isoleucine Leucine Lysine Methionine Phenylalanine Threonine Valine Total E Nonessential amino acids (NE) Aspartic Acid Serine Glutamic acid Proline Glycine Alanine Cystine Tyrosine Arginine Total NE Total amino acid E/NE ratio Table 5. Seasonal variation in mineral content (g/00 g) of the muscle of mandarin fish Siniperca scherzeri Minerals Mar. Apr. May Jun. Jul. Aug. Sep. Oct. Nov. Ca 35.2± ± ± ± ± ± ± ± ±2.45 P 230.4± ± ± ± ± ± ± ± ±0.26 Mg 23.9± ± ± ± ± ± ± ± ±0.29 Na 70.3±.9 2.5± ± ± ± ± ± ± ±8.58 K 38.5± ± ± ± ±. 3.6± ± ± ±.29 Fe 0.4± ± ± ± ± ± ± ± ±0.07 Zn 0.59± ± ± ± ± ± ± ± ±0.0 Cu 0.08±0.02 ND 0.02±0.03 ND ND ND ND 0.07± ±0.0 Se 0.06± ± ± ± ± ± ± ±0.04 ND S 230.4± ± ± ± ± ± ± ± ±0.8 ND: not detected mg/00 g, mg/00 g mg/00 g mg/00 g 0, mg/00 g,, mg/00 g mg/00 g

6 790 심길보ㆍ김민아ㆍ윤나영ㆍ송미영ㆍ정수정ㆍ임치원 Cholesterol (mg/00 g) a bc mg/00 g. 콜레스테롤함량변화 3 79 mg/00 g, 9 5 mg/00 g mg/00 g, 27.9 mg/00 g (Fig. ). Ha and Kang (990) 53. mg/ g, mg ( mg/00 g), 30.3 mg/00 g (Jeong et al., 998). 0. 비타민 A, E, niacin 함량변화 b bcd Sampling period (month) bcde Mar Apr May Jun Jul Aug Sep Oct Nov Fig.. Seasonal variation in cholesterol content (g/00 g) of the muscle of mandarin fish Siniperca scherzeri. Different superscripts on the bar are significantly different by Duncan's multiple range at P<0.05. A E (Table 6). A g/00 g, g/00 g, , 7. g/00 g,. E 3-4 6, mg/00 g mg/00 g. Jeong et al. (998) E -tocopherol 0. mg/00 g. (Jeong et al., 998), 5 2, 2 E. cde de e cde Table 6. Seasonal variation in vitamin A, E and niacin content of the muscle of mandarin fish Siniperca scherzeri Month Vitamin A (μi/00 g) Vitamin E (mg/00 g) A, E 0.2 mg/00 g (Oh et al., 2008), A 00 mg/00 g ( ) (Cho et al., 2006). E 4-7 mg/00 g ( ), (Anguilla japonica) 2-37 mg/00 g ( ) (Furuta et al., 2009). niacin, mg/00 g, 3.5 mg/00 g (Table 6).,,,.,. Niacin.,,. 사사 Niacin (mg/00 g) Mar. ND ND 2.56±0.02 cd2 Apr. ND ND 2.92±0.2 bc May 3.20± ± ±0.03 bcd Jun. ND ND 2.58±0.7 cd Jul. 3.57± ± ±0.5 d Aug. ND 0.63± ±0.0 b Sep. ND 0.26± ±0.26 b Oct. 3.86± ± ±0.6 ab Nov. 7.± ± ±0.6 a Mean±standard deviation. 2 Different superscripts within a same column are significantly different by Duncan's multiple range at P< (R206060),.

7 쏘가리의계절별영양성분변화 79 References AOAC Official Methods of Analysis. 6th Ed. Association of Official Analytical Chemist, Arlington, VA, U.S.A. Baek SH, Yoon JD, Kim JH, Park SH, Lee JW, Jang MH, Kim SK and Byeon MS Evaluation of the movement pattern of Siniperca scherzeri using the radio telemetry in the middle part of the Geum-river where wiers were constructed recently. Korean J Ecol Environ 48, org/0.64/ksl Bligh, EG and Dyer WJ A rapid method for total lipid extraction and purification. Can J Biochem Physiol 37, Cho YJ, Jung HJ, Kim YC, Oh SM, Son MJ, Kim SM and Shim KB Optimal process of eel hot-water for proper edible volume. J Fish Mar Sci Edu 8, Choi JH, Ro JI and Pyeun JH Studies on lipids in freshwater fishes 3. Distribution of lipid components in various tissues of eel, Anguilla japonica. Bull Korean Fish Soc 7, Chu W, Li W, Wang K, Liu L, Leng X and Zhang J Comparative studies on muscle cellularity and flesh quality of two mandarin fish species, Siniperca chuatsi and Siniperca scherzeri. J Food Agric Environ, FAO (Food and Agriculture Organization) Protein Requirements. FAO Nutritional Studies 6. Furuta H, Ishida T, Suzuki T, Unuma T, Kurokawa T, Sugita T and Yamamoto T Vitamin content and quality of eggs produced by broodstock injected with vitamins C and E during ratified maturation in Japanese eel Anguilla japonica. Aquaculture 289, Gye HJ, Shim KB, Lim CW, Song MY, Kim DH, Kim BK and Cho YJ Nutritional assessment and mineral content of wild and cultured catfish Silurus asotus. J Fish Marine Sci Edu 27, JFMSE Ha BS and Kang DS Studies on the lipid aquatic products (part 5), Comparison of flesh lipid composition of some fresh water fishes, mandarin fish, Korean Perch, Cornet fish and cat fish. J Korean Soc Food Nutr 9, Jeong BY, Choi BD and Lee JS Proximate composition, cholesterol and α-tocopherol content in 72 species of Korean fish. J Korean Fish Soc 3, Kang SJ, Choi BD and Jeong WG Comparison of amino acid profiles and lipids of two strains of common carp, Cyprinus carpio. J Aqua 5, Kim JD and Kang KY Coloured fishes of Korea. Academy publishing company, Seoul, Korea, 477pp. Korea Statistical Information Service (KOSIS) Year of fishery products. Retrieved from kr/statisticslist/statisticslist_0list.jsp?vwcd=mt_ ZTITLE&parentId=F#SubCont on Oct. 20. Kim KS and Lee EH Food components of wild and cultured fresh water fishes. Korean J Fish Aquat Sci 9, Rural Development Administration (RDA). 20. Korean Standard Food Composition Tables, 8th revision. RDA, Suwon, Korea, 572. Kwak BM, Lee KW, Ahn JH and Kong UY Simultaneous Determination of Vitamin A and E in Infant Formula by Rapid Extraction and HPLC with Photodiode Array Detection. J Korea Food Sci Technol 36, Lee WO, Jang SI, Lee JY and Song SJ Comparison of morophological and chromosomal charateristics and cross breeding of the two types Korean mandarin fish, Siniperca scherzeri. Kor J Ichthyol 9, Lee YA, Lee BM, Choi KC, Park YP and Bang IC Early gonadogenesis and sex differentiation of the mandarin fish Siniperca scherzeri. J Aquaculture 8, Lee JH, SK Ku, KD Park and HS Lee An immunohistochemical study on the endocrine cells in the gastrointestinal tract of the Mandarin fish (Siniperca scherzeri). Korean J Veterinary Res 42, MFDS (Ministry of Food and Drug Safety) Food Code. Sejong, Korea. Oh HT, Kim SH, Yoo SJ, Choi HJ, Chung MJ and Ham SS Component analysis of masou salmon (Oncorhynchus masou). J Korean Soc Food Sci Nutr 37, Park IW, Hong JS, Lee KK, Kim MK, Kim JB and Kang KH Histological observation on the seasonal changes of distribution of muscle components in hernant fish. Korean J Ichthyol 7, Park SY and Kim HR Changes of food components and lipid peroxides in rainbow trout with growth. J Korean Soc Food Sci Nutr 25, Steel RGD and Torrie JH Principle and procedure of statistics; a biometrical approach (2nd ed.). MacGraw-Hill Book Company, New York, U.S.A. Yang ST and Lee EH Taste compounds of fresh-water fishes-6. Taste compounds of Korean catfish meat. Korean J Fish Aquat Sci 6,

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한수지 49(6), , 2016 Original Article Korean J Fish Aquat Sci 49(6), ,2016 자연산과양식산동자개 (Pseudobagrus fulvidraco) 의시기에따른영양성분변화 임치원 김민아 계현진 윤나영 한수지 49(6), 779-784, 2016 Original Article Korean J Fish Aquat Sci 49(6),779-784,2016 자연산과양식산동자개 (Pseudobagrus fulvidraco) 의시기에따른영양성분변화 임치원 김민아 계현진 윤나영 송미영 1 심길보 * 국립수산과학원식품위생가공과, 1 국립수산과학원중앙내수면연구소 Seasonal