ISSN (Print) ISSN (Online) CASE REPORT Korean J Parasitol Vol. 49, No. 4: , December

Size: px

Start display at page:

Download "ISSN (Print) ISSN (Online) CASE REPORT Korean J Parasitol Vol. 49, No. 4: , December"

홍표 설
5 years ago
Views:

1 ISSN (Print) ISSN (Online) CSE REPORT Korean J Parasitol Vol. 49, No. 4: , December Recurrent Hepatic lveolar Echinococcosis: Report of The First Case in Korea with Unproven Infection Route Su-Jin Kim 1, Jong-Han Kim 2, Sang-Young Han 2, Young-Hoon Kim 3, Jin-Han Cho 4, Jong-Yil Chai 5 and Jin-Sook Jeong 1, * Departments of 1 Pathology, 2 Internal Medicine, 3 General Surgery, and 4 Radiology, Dong- University College of Medicine, usan , Korea; 5 Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul , Korea bstract: Human alveolar echinococcosis (E), a hepatic disorder that resembles liver cancer, is a highly aggressive and lethal zoonotic infection caused by the larval stage of the fox tapeworm, Echinococcus multilocularis. E. multilocularis is widely distributed in the northern hemisphere; the disease-endemic area stretches from north merica through Europe to central and east sia, including northern parts of Japan, but it has not been reported in Korea. Herein, we represent a first case of E in Korea. 41-year-old woman was found to have a large liver mass on routine medical examination. The excised mass showed multinodular, necrotic, and spongiform appearance with small irregular pseudocystic spaces. Microscopically, the mass was composed of chronic granulomatous inflammation with extensive coagulation necrosis and parasite-like structure, which was revealed as parasitic vesicles and laminated layer delineated by periodic acid-schiff (PS) stain. Clinical and histologic features were consistent with E. fter 8 years, a new liver mass and multiple metastatic pulmonary nodules were found and the recurred mass showed similar histologic features to the initial mass. She had never visited endemic areas of E, and thus the exact infection route is unclear. Key words: Echinococcus multilocularis, alveolar echinococcosis, hepatic, human INTRODUCTION Echinococcus multilocularis is the fox tapeworm and the most pathogenic one among the 4 zoonotic species of the genus Echinococcus, and thus E. multilocularis can cause an infection resulting in a potentially fatal chronic liver infection called human alveolar echinococcosis (E) [1]. E. multilocularis has an extensive geographic range, mainly across the northern hemisphere, including endemic regions in central Europe, most of northern and central Eurasia, extending eastward to Japan and parts of North merica [1,2]. Foxes and other canids are the natural definitive hosts of E. multilocularis. The intermediate hosts, most ly rodents and ungulates including humans, are infected by accidental ingestion of eggs shed in the feces of the definitive hosts [3]. The metacestodes go through the small intestine and mostly reach the liver, but also other organs such Received 29 ugust 2011, revised 22 September 2011, accepted 19 November * Corresponding author (jsjung1@dau.ac.kr) 2011, Korean Society for Parasitology This is an Open ccess article distributed under the terms of the Creative Commons ttribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. as the lung and brain. The definitive hosts are infected by ingestion of parasitized organs of the intermediate hosts. Human E is a potentially fatal, chronically progressive hepatic infection with E. multilocularis, that is characterized by a long asymptomatic incubation period during which an invasive tumor-like multi-vesicular and exogenously budding masslike lesion is developed. The parasitic lesion composes of dense granulomatous infiltration and microcalcifications microscopically, and eventually necrotic cavitation may occur [1]. In Korea, E has never been reported, and herein, we report the first written case of human hepatic E in a southeastern Korean district, Gimhae-si (city). CSE RECORD 41-year-old woman residing in Gimhae-si, Gyeongsangnam-do, Korea transferred to Dong- University Hospital on 5 pril 2002 for evaluation of the known hepatic mass. She had no significant history of past illness, except heavy alcohol consumption. She denied any contact history with domestic animals or pets as well as livestock, and has not visited zoo and never traveled abroad. On 17 September 2001, she visited a re- 413

414 Korean J Parasitol Vol. 49, No. 4: 413-418, December 2011 gional hospital for check-up her epigastric pain.

t Dong- University Hospital, the liver showed an 8 cm sized ill-defined mass with 1.

1, ) and no or poor enhancement after contrast injection. These radiologic findings suggested an inconclusive hepatic tumor with necrosis.

2 414 Korean J Parasitol Vol. 49, No. 4: , December 2011 gional hospital for check-up her epigastric pain. Routine radiologic examinations revealed a hepatic mass, and the pathologic findings of needle biopsied mass were described as coagulation necrosis. t Dong- University Hospital, the liver showed an 8 cm sized ill-defined mass with 1.5 cm and 3 cm sized satellite nodules at the left lateral segment of the liver without lymph node enlargement on CT imaging. Contrast enhanced CT scan demonstrated low attenuated mass (Fig. 1, ) and no or poor enhancement after contrast injection. These radiologic findings suggested an inconclusive hepatic tumor with necrosis. lthough non-neoplastic mass was considered, malignancy such as cholangiocarcinoma should be ruled out radiologically. Laboratory data from routine CC, chemistry, serology, electrolyte profiles, hormone analysis of blood, tumor markers (α-fetoprotein, carcinoembryonic antigen, and C 19-9), and urine analysis were all within normal limits. To rule out any possibility of malignancy, left hepatectomy was done. During operation, frozen section made by the biopsied hepatic mass revealed necrotizing granulomatous inflammation. The lobectomized liver showed a relatively demarcated mass, 11 cm in greatest dimension and abutted several nodular masses of 1-3 cm in diameter, which showed necrosis on cut surface, grossly. Histologically, extensive coagulation necrosis, granulomas, and dispersed acellular membranous shells within cystic spaces were observed. These gross and histologic findings corresponded with a parasitic infection of the liver, but identification of the specific parasite species was not processed. She was discharged without complications. During follow-up, on 19 July 2005, a new nodular mass, cm was observed in S5/8 segment of post-hepatectomized liver on CT imaging, and a needle biopsy of the mass showed coagulation necrosis. The hepatic mass was checked to be enlarged to cm and several small nodules in both basal lungs on 7 ugust The hepatic mass showed extensive necrosis, and the pulmonary nodules represented calcification C D Fig. 1. Contrast enhanced abdominal CT scan ( and, imaging at 1st operation; C and D, imaging at 2nd operation; and C, arterial phase; and D, portal phase). CT reveals a low attenuated huge mass (arrows) with little difference between arterial and portal phases.

contrast enhanced CT scan. Imaging study showed the same features of the previous mass (Fig. 1C, D).

calcified granu- lomas, and metastasis, even less likely (Fig. 2). The eosinophilic count was 8.

helminths, including scaris lumbricoides, Trichuris trichiura, Enterobius vermicularis, hookworms, and protozoa.

3 Kim et al.: case of recurrent hepatic alveolar echinococcosis 415 and focally impinged air, suggesting calcified granuloma rather than metastatic malignancy. On 7 May 2010, the hepatic mass was more enlarged, up to 10 cm in diameter and right supradiaphragmatic lymph node enlargement was found on contrast enhanced CT scan. Imaging study showed the same features of the previous mass (Fig. 1C, D). On the lung setting of chest CT scan, relatively well defined small nodules were seen in both lower lobes of the lung, suggestive for calcified granu- lomas, and metastasis, even less likely (Fig. 2). The eosinophilic count was 8.9% (reference value: 0-7%), total IgE was 1,944 ku/l (reference value: ku/l), and parasitological studies were all negative for helminths, including scaris lumbricoides, Trichuris trichiura, Enterobius vermicularis, hookworms, and protozoa. second operation, including a partial anterior resection, was done but the hepatic mass was not completely removed. Fig. 2. Chest CT scan. Small nodules (arrows) are seen in the left lower lobe of the lung. C Fig. 3. Gross findings of the liver mass (, higher magnification of the white box of ). External ( and ) and cut (C) surfaces show greenish yellow colored necrotic tissues with mostly microcystic and sometimes large cystic spaces. Glisson s capsule is thickened, showing fibrosis. Grossly normal liver tissues are not seen.

416 Korean J Parasitol Vol. 49, No. 4: 413-418, December 2011 The resected liver mass, measuring 16.2 9.5 4.

liver was not present. The cut surfaces on serial sections were spongy, with mostly small and occasionally large cystic spaces (Fig. 3).

ny intact parasite body was not identified. fragment of the normal liver, 1.5 0.8 cm was separately submitted.

The cysts contained ribbon-like eosinophilic laminated layers, characteristic for E. multilocularis [4], which appeared more strongly red by periodic acid-schiff (PS) stain (Fig. 4).

Chronic granulomatous inflammation, composing of proliferating epithelioid cells surrounding the organisms, and infiltration of lymphoplasma cells, a few eo- sinophils, and giant cells were observed

We could approach this unusual hepatic mass, mimicking clinical features of malignancy after consultation to Professor Jong-Yil Chai, Department of Parasitology and Tropical Medicine, Seoul National

4 416 Korean J Parasitol Vol. 49, No. 4: , December 2011 The resected liver mass, measuring cm was rim med by raggedy, thickened, fibrotic or myxoid Glisson s capsule, focally adhered with fat tissues, and underlying parenchyme showed total necrosis in greenish yellow color, and normal liver was not present. The cut surfaces on serial sections were spongy, with mostly small and occasionally large cystic spaces (Fig. 3). The cystic spaces were empty or filled with mucoid exudates, and inner surface was rimmed by more yellow wall. etween the cysts, yellowish grey rather solid tissues were occupied. ny intact parasite body was not identified. fragment of the normal liver, cm was separately submitted. Microscopically, the mass showed diffuse and extensive necrosis with totally effaced liver tissues. mong necrosis, numerous variable-sized multilocular cysts with discernible lining were observed. The cysts contained ribbon-like eosinophilic laminated layers, characteristic for E. multilocularis [4], which appeared more strongly red by periodic acid-schiff (PS) stain (Fig. 4). Occasionally, fine calcifications were identified between cystic areas. Chronic granulomatous inflammation, composing of proliferating epithelioid cells surrounding the organisms, and infiltration of lymphoplasma cells, a few eo- sinophils, and giant cells were observed at the periphery of the mass (Fig. 5). We could approach this unusual hepatic mass, mimicking clinical features of malignancy after consultation to Professor Jong-Yil Chai, Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul, Korea. We concluded that these findings corresponded well with hepatic alveolar echinococcosis although Korea is not an endemic area for this cestode disease. She was discharged after the second operation without comlpication. During about 14 months of follow-up period, she has taken 800 mg of albendazole, orally in 2 divided doses, without any symptom. Follow-up radiologic tests showed unremarkable changes of the remaining mass on the remnant liver, and slightly decreased size of pulmonary nodules. DISCUSSION E is restricted to transmission in the northern hemisphere as mentioned. lthough, Hokkaido of Japan s northmost island is known as an endemic area [1,3] and in these days, the C D E F Fig. 4. Microscopic findings of the liver mass (-C, H-E stain; D-F, PS stain; and E, higher magnification of the black box of and D; C and F, higher magnification of the white box of and E). The mass shows diffuse and extensive necrosis with variable-sized multilocular cystic spaces which includes strong PS positive ribbon-like and lamellated structures consistent with E.

Furthermore, in non-endemic areas, E is enough to be confused with malignant tumor and misdiagnosed.

5 Kim et al.: case of recurrent hepatic alveolar echinococcosis 417 Fig. 5. Microscopic findings of the periphery of the liver mass (, H-E stain;, PS stain). The outer portion of necrosis shows chronic granulomatous inflammation with microcystic organisms. endemic areas have expanded, in Korea, human E is not notified to our knowledge. Furthermore, in non-endemic areas, E is enough to be confused with malignant tumor and misdiagnosed. We experienced an unusual clinical case, complaining of a hepatic mass with histological features of non-neoplastic and suggestive of a parasite infection and continuous clinical suspicion of malignant tumor for 10 years. Even she has been residing in Gimhae city which is a recently developed and industrialized area, but was a rural community, she denied any history of contact with domestic animals or pets as well as livestock and has been never visited other countries. In this regard, it is strongly suggested that a possible infection route from imported animals may be underlaid. Concerning a literature review of human cestode infections in Korea, Min [5] recognized that most human hydatidosis cases have been imported ones, from outside of Korea, except 1 possible indigenous case [6], and they were all caused by cystic hydatidosis (CE) due to Echinococcus granulosus, not E. E is rare but fatal. It has a risk of metastasis in advanced stages, usually resulting in secondary lesions in the lung or brain. fatal outcome may occur in over 95% of untreated patients within a 10-year period following diagnosis [1]. When a slowly growing tumor, visualized by imaging techniques, is detected, a case is verified if at least 2 of the following 4 pa- rameters are fulfilled, according to the WHO recommendations: 1) Typical organ lesions detected by imaging techniques, e.g., abdominal ultrasound, CT, and magnetic resonance imaging, 2) Detection of E. multilocularis specific serum antibodies by serotests, 3) Histopathology compatible with E, 4) Detection of E. multilocularis nucleic acid in a clinical specimen [2,7]. CT imaging of slow growing and recurred mass in this case displayed ill-defined hypodense mass with poor or no enhancement after contrast injection and no differences on arterial and portal phase, which is characteristic for hepatic E [8]. During the follow-up period of our case, multiple basal nodules at both lungs appeared, showing pulmonary extension. However, it is sometimes difficult to rule out other parasitic infections, pathologically and clinically, especially in non-endemic areas. Multiple cysts, mostly empty and histologically lamellated mem brane along the inner side of the cysts with characteristic PS (+) stain, are compatible with E [2,4,8]. Moreover, supradiaphragmatic lymph node involvement on CT imaging occurred in our case. Lymphatic spread has been hypothesized to constitute a route for dissemination [9,10], however, the actual human involvement of lymph nodes in E has not been frequently reported [10]. In our case, lymph node biopsy with studies of E was not done. We had not performed serologic and molecular studies for detection of E. multicularis-specific serum antibodies and nucleic acids until just

6 418 Korean J Parasitol Vol. 49, No. 4: , December 2011 prior to publication of this paper. However, these studies were subsequently performed and the results will be published elsewhere. In endemic areas, E is considered the most serious parasitic zoonosis and E incidence may increase in the future though the incidence is different among regions. Thus, molecular and immunological technology for detection of humans and animals infected with E. multicularis as well as integrated control measures have been advanced, centering around Japan [11]. Since 1996, guidelines for the treatment of human E are suggested and documented as a manual [11] and have recently been updated by WHO-IWGE [7]. The rules have been set: radical surgery is the first choice in all cases suitable for total resection of the lesion; benzimidazoles as anthelmintic drugs are mandatory in all patients, temporarily after complete resection of the lesions, and for life in all other cases [2,7,12]. Interventional procedures should be preferred to palliative surgery whenever possible. In our patient, 2 times radical operations were proceeded because hepatic malignancy could not continuously excluded. fter the 2nd operation, albendazole was recommended. lbendazole has an efficacy similar to mebendazole, the first successfully used benzimidazole derivative [13] together with some pharmacokinetic advantages [14]. She has been visiting regularly for radiologic follow-up, but till now no remarkable changes have been reported. Here, we report a case of human E, representing unusual hepatic mass on clinical examination which showed necrosis with numerous sterile cysts and granulomas on pathologic examination, in a non-endemic area with uncertain infection route. REFERENCES 1. Craig P. Echinococcus multilocularis. Curr Opin Infect Dis 2003; 16: Kern P. Clinical features and treatment of alveolar echinococcosis. Curr Opin Infect Dis 2010; 23: Eckert J, Deplazes P. iological, epidemiological, and clinical aspects of echinococcosis, a zoonosis of increasing concern. Clin Microbiol Rev 2004; 17: Orihel TC, sh LR. Parasites in Human Tissues. Chicago, Illinois, US. SCP Press. 1995, p Min DY. Cestode infections in Korea. Korean J Parasitol 1990; 28(suppl): Chung KY, Lee DY, Hong PH, Choi IJ, Min DY. Surgical treatment of pulmonary hydatid cysts-two case report-. Korean J Thorac Cardiovas Surg 1983; 16: runetti E, Kern P, Vuitton D, Writing Panel for the WHO-IW- GE. Expert consensus for the diagnosis and treatment of cystic and alveolar echinococcosis in humans. cta Trop 2010; 114: Czermak V, khan O, Hiemetzberger R, Zelger, Vogel W, Jaschke W, Rieger M, Kim SY, Lim JH. Echinococcosis of the liver. bdom Imaging 2008; 33: Reuter S, Seitz HM, Kern P, Junghanss T. Extrahepatic alveolar echinococcosis without liver involvement: rare manifestation. Infection 2000; 28: uttenschoen K, Kern P, Reuter S, arth TFE. Hepatic infestation of Echinococcus multilocularis with extension to regional lymph nodes. Langenbecks rch Surg 2009; 394: Ito, Romig T, Takahashi K. Perspective on control options for Echinococcus multicularis with particular reference to Japan. Parasitology 2003; 127: S159-S Eckert J, Gemmell M, Meslin FX, Pawlowski ZS. WHO/OIE Manual on Echinococcosis in Humans and nimals: Public Health Problem of Global Concern. World Organisation for nimal Health. 2001, p Kern P. Human echinococcosis: Follow-up of 23 patients treated with mebendazole. Infection 1983; 11: Horton J. lbendazole for the treatment of echinococcosis. Fundam Clin Pharmacol 2003; 17:

(49-54)Kjhps004.hwp

(49-54)Kjhps004.hwp Biliary Cystadenoma and Cystadenocarcinoma of the Liver Jae Hoon Lee, M.D., Dong Ho Choi, M.D. 1, Kyeong Geun Lee, M.D., Hwon Kyum Park M.D. and Kwang Soo Lee, M.D. Department of Surgery, College of Medicine,