한수지 49(6), 785-79, 206 Original Article Korean J Fish Aquat Sci 49(6),785-79,206 쏘가리 (Siniperca scherzeri) 의시기에따른영양성분변화 심길보 * 김민아 윤나영 송미영 정수정 임치원 국립수산과학원식품위생가공과, 국립수산과학원중앙내수면연구소 Seasonal Variation in the Nutritional Composition of the Muscle of Mandarin Fish Siniperca scherzeri Kil Bo Shim*, Min A Kim, Na Young Yoon, Mi Young Song, Su Jung Jung and Chi Woon Lim Food Safety and Processing Research Division, National Institute of Fisheries Science, Busan 46083, Korea Central Inland Fisheries Research Institute, National Institute of Fisheries Science, Gapyeong 2453, Korea Seasonal variation in the composition and fatty acid, amino acid, cholesterol, and vitamin contents in the muscles of the mandarin fish Siniperca scherzeri, caught in Soyangho Lake, Korea, was studied. The moisture content was 74.5-78.7 g/00 g, lipids ranged between.06 and 4.23 g/00 g, protein between 8.5-20.7 g/00 g, and ash between 0.74 and 2.04 g/00 g. The moisture content was lowest in May and tended to increase from June to October. The highest protein levels occurred in May, which coincided with the timing of oocyte maturation, and then decreased at the beginning of spawning. The highest lipid levels occurred in May and November coinciding with the periods before spawning and hibernation. The ratio of EAA/NEAA was higher than the value recommended by FAO for an ideal food protein. There were significant differences in Mg and Fe contents with spawning; they were highest in May. Contents of vitamin A and E were from not detected (ND) to 7. µg/00 g and ND to 0.69 mg/00 g, respectively and were highest in November. The content of niacin tended to decrease in June and increased until November and hibernation. The cholesterol content was 4.9-78.9 mg/00 g; it decreased from March to July and then increased until November. In conclusion, there is potential for expanding the commercial utilization of the mandarin fish as a food resource. Key words: Mandarin fish, Seasonal variation, Proximate composition, Niacin, Hibernation 서론 (Siniperca scherzeri) (Order Perciformes),,.,,, (Lee et al., 2005; Kim and Kang,993;Lee et al.,997)., 5-7, 5, 6 (Baek et al., 205; Lee et al., 2002)., (Baek et al., 205). 205 33,056, 26,237., 04 (KOSIS, 206).,,,,, (Gye et al., 205; Oh et al., 2008; Yang and Lee, 983; Choi et al., 984; Kim and Lee, 986; Park and Kim, 996; Kang et al., 992), http://dx.doi.org/0.5657/kfas.206.0785 Korean J Fish Aquat Sci 49(6) 785-79, December 206 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Licens (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Received 28 October 206; Revised 22 November 206; Accepted 29 November 206 *Corresponding author: Tel: +82. 5. 720. 2650 Fax: +82. 5. 720. 2669 E-mail address: kilbo22@korea.kr Copyright 206 The Korean Society of Fisheries and Aquatic Science 785 pissn:0374-8, eissn:2287-885
786 심길보ㆍ김민아ㆍ윤나영ㆍ송미영ㆍ정수정ㆍ임치원 (Ha and Kang, 990),.,,,,,,. 재료 재료및방법, 2 2. 3, Table. 일반성분 AOAC (995), Kjeldahl, Soxhlet,. 지방산조성 Bligh and Dyer (959) 5 chloroform:methanol (2:, v/v), 4% BF 3 -methanol methyl ester, gas chromatography (GC; Shimazu GC 200 Plus, Shimazu, Tokyo, Japan). 총아미노산함량 200 mg 6 N HCl 24 0., ph 2.2 50 ml 0.20 m membrane filter,. (Sykam DE/S-433D, Sykam, Eresing, Germany). 미네랄함량 g 65% (Suprapur grade, Merck, Darmstadt, Gremany) 0 ml 80 80 400. 0 ml. 2% (ADVANTEC No.5C, Tokyo, Japan) 00 ml Inductively coupled plasma spectrometry (ICP; Optima 3300XL, PerkinElmer, Waltham, MA, USA). (MFDS, 204). 콜레스테롤함량 2-3 g 95% ethanol 40 ml 8 ml 50% KOH 80-90 70. 95% ethanol 60 ml 5 24. toluen 00 ml 30 500 ml M KOH 0 ml 0 0.5 M KOH 40 ml 0. toluene 40 ml toluene. toluene 20 g 40 3 3 ml. 3 ml dimethylformamide ml 0.2 ml hexamethyldisilane 0. ml trimethylchlorosilane 5 -cholestane (MFDS, 205). HP- (30 m 0.25 mm 0.25 m, Agilent, USA) gas chromatography (GC; Agilent 7890, Agilent, CA, USA) (MFDS, 205). 비타민 A, E 함량 A, E Kwak et al. (2004). 3 g ethanol 30 ml, 60% KOH 3 ml, 0% pyrogallol ml 30, 30 ml. petroleum ether, petroleum ether 50 ml 2. petroleum ether 0 ml phenolphthalein, petroleum ether sodium sulfate. Petroleum ether 25 C methanol 5 ml 0.25 m membrane filter. Develosil RP-Aqueous C30 (4.6 250 mm, 5 m particle size, Phenomenex, Tokyo, Japan) HPLC (Surveyor Plus HPLC system, Thermo, CA, USA).
쏘가리의계절별영양성분변화 787 Niacin 함량 5-0 g 5 mm Sodium hexanesulfonate 50 ml. 30 600 rpm 30, (4, 3,699 g 5 ) 0.2 µm membrane syringe filter (MFDS, 205). C8 column (250 4.6 mm 5 m, Waters, USA) A: 5 mm hexanesulfonate/0.% acetic acid, B: 35% 5 mm hexanesulfonate/0.% acetic acid 65% MeOH,.0 ml/min, 0 L 260 nm HPLC (Surveyor Plus HPLC system, Thermo, CA, USA). 통계처리, Duncan s multiple range test (P<0.05) (Steel and Torrie, 980). 결과및고찰 가식부수율및일반성분변화 62.2-78.9%, 3 6 (Table ). Table 2. 74.5-78.7 g/00 g, 5 74.5 75.0 g/00 g (P>0.05)..06-4.23 g/00 g, 5 3.46 g/00 g, 6 7.69,.06 g/00 g. 4.23 g/00 g. 8.5-20.7 g/00 g, 0 4. 0.74-2.04 g/00 g, 8 0.74 g/00 g 3 2.04 g/00 g.,, 4 7 0 3 (Park et al., 995)., (Park et al., 995)., 5,,. (Siniperca chuatisi, Siniperca scherzeri) Table. The capture date and biological data of mandarin fish Siniperca scherzeri Month Total length (mm) Total weight (g) Yield (%) Mar. 270.5±35.2 228.8±02. 75.3±9.07 ab) Apr. 287.±9.9 285.6±49.9 65.4±5.33 abc May 237.0±3.5 9.2±36.4 65.0±.42 abc Jun. 223.5±24.5 40.3±44.3 53.4±8.0 c Jul. 278.0±5.7 245.7±9.6 70.0±3.8 ab Aug. 295.0±24.9 356±94.6 78.9±6.8 a Sep. 336.7±55.8 472.6±24.6 62.4±.59 bc Oct. 25.7±2. 223.3±29.9 77.7±7.3 ab Nov. 279.0±6.4 269.6±40.9 62.2±2.50 bc Different superscripts within a same column are significantly different by Duncan's multiple range at P<0.05. Table 2. Seasonal variation in proximate composition content (g/00 g) of the muscle of mandarin fish Siniperca scherzeri Month Moisture Crude lipid, 2 g/00 g, 8.4-9.8 g/00 g (Chu et al., 203), 79.7 g/00 g, 0.9 g/00 g, 0.8 g/00 g, 5.7 g/00 g (Ha and Kang, 990)., 5. 지방산조성비변화 Crude protein Ash Mar. 77.9±0.49 a2 2.23±0.09 d 9.±0.25 c 2.04±0.32 a Apr. 77.±0.36 ab 2.99 c 8.5±0.6 d.38±0.03 b May 74.5±0.6 b 3.46±0.26 b 20.7±0.38 a 0.98±0.05 cd Jun. 78.0±0.5 a.69±0.06 e 9.7±0. b.05±0.06 c Jul. 78.7±0. a.06±0.09 f 9.8±0.2 b.52±0.2 b Aug. 76.9±3.94 ab.2±0.9 f 9.2±0.24 c 0.74±0.05 d Sep. 78.6±0.38 a.94±0.09 e 8.9±0.8 cd 0.99±0.05 dc Oct. 76.6±0.4 ab.92±0.6 e 20.7±0.45 a.23±0.2 bc Nov. 75.0±0.4 b 4.23±0.09 a 9.9±0.32 b.06±0.22 c Mean±standard deviation. 2 Different superscripts within a same column are significantly different by Duncan's multiple range at P<0.05. Table 3., palmitic acid (C6:0), oleic acid (C8:), linoleic acid (C8:2n6), docosahexaenoic acid (C22:6n-3) eicosa-
788 심길보ㆍ김민아ㆍ윤나영ㆍ송미영ㆍ정수정ㆍ임치원 Table 3. Seasonal variation in fatty acid composition (%) of the muscle of mandarin fish Siniperca scherzeri Fatty acid Mar. Apr. May Jun. Jul. Aug. Sep. Oct. Nov. C4:0 4.08 3.94 2.59 2.65 3.67.83 2.83 2.99 3.59 C6:0 6. 5.2 7 7.2 8.5 20.7 9.5 9.6 7.8 C8:0 2.90 2.74 4.3 5.25 4.65 5.54 4.2 4.39 3.29 Saturated 25.9 24.4 26.5 27.9 3. 32. 30.2 29.2 27.4 C6:.2.5 8.46 8.57 9.45 0.2 4.3 3.8 0.4 C8: 22.4 23.8 27.7 24 27.8 27 26. 20. 22. C20: 0.40 0.42 0.70 0.74 0.75.36 0.96 0.70 0.42 Monoen 34.5 36. 37. 33.5 38.3 39.2 42 35.6 33.2 C8:2n-6 5.72 5.73 6.20 5.4 5.46 4.70 4.57 3.08 5.52 C8:3n-3 4.02 4.24 3.55 3.2 2.69 3.9 4.6 4. 4.75 C8:4n-3.67.83.00.00.00 0.37 0.7 0.57 2.0 C20:4n-6 5.74 5.4 5.3 5.4 4.7 2.77 2.04 2.9 5.05 C20:5n-3(EPA) 6.48 7.8 5.6 4.97 3.65 3.28 3.78 5.95 6.3 C22:6n-3DHA) 7.40 6.84 7.25 9.07 5.85 4.98 3.85 8.58 7.75 Polyen 39.7 39.4 36.4 38.5 30.6 28.7 27.8 35.2 39.5 pentaenoic acid (C20:5n-3). 27.8-39.7%, 3 8 9 0 39.45%. 24.4-32.%, 33.2-42%. 8 9. EPA 3 6.48% 7 8 3.65% 3.28%. DHA 6 9.07% 9 3.85%, 7.75%. C8:0 9 20.7%, 7.8%. 총아미노산함량변화 5.9-9. g/00 g, leucine, lysine, aspartic acid, glutamic acid, alanine, arginine. 5 9. g/00 g, 7 5.9 g/00 g (Table 4). 7.0-8.09 g/00 g, 9 8.09 g/00 g. 8.89-0.6 g/00 g, 9. 0.75-0.82, FAO (FAO, 957). 0.64-0.68, (Chu et al., 203). 미네랄함량변화 Table 5.,,, 230.4-498 mg/00 g, 273.8-394.5 mg/00 g, 77.7-294. mg/00 g. 357.9 2. mg/00 g, 226.2.3 mg/00 g (Oh et al., 980), 263 mg/00 g, 400 mg/00 g (RDA, 20), 323.88-379.40 mg/00 g, 93.74-223.02 mg/00 g (Gye et al., 205),, 7,,. 3 23.9 mg/00 g 7 38. mg/00 g,.. 3 35.2 mg/00 g 4 3.6 mg/00 g,. 4 308.3 mg/00 g, 200. mg/00 g. 3 38.5 mg/00 g 273.8 mg/00 g
쏘가리의계절별영양성분변화 789 Table 4. Seasonal variation in amino acid content (g/00 g) of the muscle of mandarin fish Siniperca scherzeri Amino acids Mar. Apr. May Jun. Jul. Aug. Sep. Oct. Nov. Essential amino acids (E) Histidine 0.58 0.54 0.58 0.58 0.52 0.43 0.45 0.52 0.53 Isoleucine 0.92 0.83 0.75 0.88 0.74 0.80 0.87.0 0.93 Leucine.46.45.4.59.37.4.66.65.55 Lysine.30.28.4.25.50.52.72.39.35 Methionine 0.50 0.45 0.55 0.38 0.55 0.54 0.62 0.63 0.63 Phenylalanine 0.85 0.8 0.78 0.7 0.73 0.76 0.87 0.9 0.87 Threonine 0.80 0.8 0.83.24 0.78 0.80 0.94 0.92 0.86 Valine 0.92 0.86 0.83.05 0.82 0.88 0.95.02 0.96 Total E 7.34 7.02 7.4 7.68 7.0 7.5 8.09 8.06 7.69 Nonessential amino acids (NE) Aspartic Acid.74.80.77.52.63.67.87.85.77 Serine 0.72 0.74 0.73 0.8 0.7 0.72 0.86 0.8 0.77 Glutamic acid 2.46 2.57 2.6 2.3 2.58 2.63 2.98 2.85 2.7 Proline 0.4 0.60 0.66 0.6 0.52 0.69 0.67 0.69 0.66 Glycine 0.8 0.04 0.07 0. 0.07 0.03 0.07 0.04 0.03 Alanine.5.3.0.36.02.0.23.24.9 Cystine 0.03 0.83 0.92 0.78 0.72 0.89 0.88 0.9 0.88 Tyrosine 0.63 0.6 0.63 0.78 0.6 0.64 0.75 0.7 0.7 Arginine.06.02.04.08.04.2.27.20. Total NE 9.00 9.33 9.53 9.33 8.89 9.48 0.6 0.3 9.82 Total amino acid 6.3 6.3 9. 7.0 5.9 6.6 8.7 8.4 7.5 E/NE ratio 0.82 0.75 0.75 0.82 0.79 0.75 0.76 0.78 0.78 Table 5. Seasonal variation in mineral content (g/00 g) of the muscle of mandarin fish Siniperca scherzeri Minerals Mar. Apr. May Jun. Jul. Aug. Sep. Oct. Nov. Ca 35.2±.68 3.6±.79 3.5±0.62 6.2±0.05 6.3±4.8 4.6±.20 6.7±0.04 23.9±3.23 4.00±2.45 P 230.4±0.62 308.4±0.45 200.9±8.2 28.5±6.05 20.3±2.00 8.±7.77 77.7±.46 294.±2.7 200.±0.26 Mg 23.9±0.56 22.2±0.24 27.±0.34 36.0±0.79 38.±0.04 32.8±0.47 32.6±0.47 34.9±2.33 23.7±0.29 Na 70.3±.9 2.5±2.6 37.2±2.79 42.2±0.96 43.±0.52 47.±.37 27.6±0.23 6.4±4.79 45.4±8.58 K 38.5±0.2 394.5±7.23 334.6±55.4 320.9±6.8 350.6±. 3.6±2.59 288.9±4.2 359.2±30. 273.8±.29 Fe 0.4±0.04 0.33±0.04 0.9±0.26 0.4±0.0 0.49±0.03 0.2±0.04 0.39±0.03 0.44±0.0 0.32±0.07 Zn 0.59±0.03 0.55±0.0 0.33±0.2 0.52±0.03 0.33±0.0 0.55±0.37 0.34±0.03 0.66±0.7 0.26±0.0 Cu 0.08±0.02 ND 0.02±0.03 ND ND ND ND 0.07±0.0 0.0±0.0 Se 0.06±0.03 0.04±0.03 0.08±0.03 0.00±0.00 0.08±0.0 0.08±0.0 0.07±0.0 0.5±0.04 ND S 230.4±0.6 66.6±0.8 469.6±204.4 498.±8.97 496.3±3.59 469.6±3.59 432.4±5.20 476±32.6 276.6±0.8 ND: not detected.. 3 0.4 mg/00 g, 6 0.4 mg/00 g 7 0.49 mg/00 g. -0.5 mg/00 g 0, 3 230.6 mg/00 g,, 276.6 mg/00 g. 3 70.3 mg/00 g
790 심길보ㆍ김민아ㆍ윤나영ㆍ송미영ㆍ정수정ㆍ임치원 Cholesterol (mg/00 g) 00 80 60 40 20 0 a bc 4 2.5 mg/00 g. 콜레스테롤함량변화 3 79 mg/00 g, 9 5 mg/00 g. 0 32.2 mg/00 g, 27.9 mg/00 g (Fig. ). Ha and Kang (990) 53. mg/ g, 4-5. 40.9 mg (.7-249 mg/00 g), 30.3 mg/00 g (Jeong et al., 998). 0. 비타민 A, E, niacin 함량변화 b bcd Sampling period (month) bcde Mar Apr May Jun Jul Aug Sep Oct Nov Fig.. Seasonal variation in cholesterol content (g/00 g) of the muscle of mandarin fish Siniperca scherzeri. Different superscripts on the bar are significantly different by Duncan's multiple range at P<0.05. A E (Table 6). A 5 3.20 g/00 g, 7 3.57 g/00 g, 2 3.86, 7. g/00 g,. E 3-4 6, 5 0.2 mg/00 g 7-0.29-0.69 mg/00 g. Jeong et al. (998) E -tocopherol 0. mg/00 g. (Jeong et al., 998), 5 2, 2 E. cde de e cde Table 6. Seasonal variation in vitamin A, E and niacin content of the muscle of mandarin fish Siniperca scherzeri Month Vitamin A (μi/00 g) Vitamin E (mg/00 g) A, E 0.2 mg/00 g (Oh et al., 2008), A 00 mg/00 g ( ) (Cho et al., 2006). E 4-7 mg/00 g ( ), (Anguilla japonica) 2-37 mg/00 g ( ) (Furuta et al., 2009). niacin, 7 2.5 mg/00 g, 3.5 mg/00 g (Table 6).,,,.,. Niacin.,,. 사사 Niacin (mg/00 g) Mar. ND ND 2.56±0.02 cd2 Apr. ND ND 2.92±0.2 bc May 3.20±.07 0.2±0.0 2.85±0.03 bcd Jun. ND ND 2.58±0.7 cd Jul. 3.57±.3 0.42±0.06 2.5±0.5 d Aug. ND 0.63±0.06 2.99±0.0 b Sep. ND 0.26±0.02 2.97±0.26 b Oct. 3.86±.77 0.35±0.04 3.23±0.6 ab Nov. 7.±3.63 0.69±0.0 3.5±0.6 a Mean±standard deviation. 2 Different superscripts within a same column are significantly different by Duncan's multiple range at P<0.05. 206 (R206060),.
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