KISEP 40 5 1997 흰쥐에서일측전정기관손상후전정안구반사의회복과내측전정신경핵에서 c-fos 단백질발현과의관계 * Abstract 황오성 이문영 김민선 박병림 이정헌 김종길 송영창 곽기용 Relationship between Recovery of Vestibuloocular Reflex and c-fos Protein Expression in Medial Vestibular Nuclei Following Unilateral Labyrinthectomy in Rats Oh Seong Whang, M.D., Moon Young Lee, M.D., Min Sun Kim, M.D., Byung Rim Park, M.D. Department of Physiology, Wonkwang University School of Medicine and Medicinal Resources Research Center of Wonkwang University, Chunbuk, Korea Jung Hun Lee, M.D., Jong Kil Kim, M.D., Young Chang Song, M.D., Kee Yong Kwak, M.D. Department of Otolaryngology, Wonkwang University School of Medicine, Chunbuk, Korea Relationships between recovery of vestibuloocular reflex and expression of c-fos immunoreactive cells in the medial vestibular nuclei following unilateral labyrinthectomyulx were investigated in rats. Frequency of spontaneous nystagmus, velocity of eye movement induced by sinusoidal rotation of the whole body at frequencies of 0.1, 0.2, 0.5Hz, and the number of c-fos immunoreactive cells in the medial vestibular nuclei were measured for 72 hours after ULX. Frequency of spontaneous nystagmus was 3.90.5 beats/secsmsd immediately after ULX and disappeared completely within 48 hours. On sinusoidal rotation, eye movement induced by rotation toward the lesioned side recovered normal pattern within 24 hours at 0.1Hz rotation, and 12 hours at 0.2, 0.5Hz. Directional preponderance which represents the symmetry of bilateral vestibular functions decreased to less than 20 at 72 hours, but did not recover normal limit. The number of c- fos immunoreactive cells in the bilateral medial vestibular nuclei was severe asymmetry till 24 hours of ULX. However, the symmetry was recovered after 48 hours. These results indicate that the 739
40 5 1997 recovery of vestibuloocular reflex correlates with the expression of c-fos immunoreactive cells of the medial vestibular nuclei in the early stage of vestibular compensation following ULX. Therefore, the vestibular nuclei may play a key role in vestibular compensation. Korean J Otolaryngol 405, 1997 KEY WORDSc-Fos protein Medial vestibular nucleus Unilateral labyrinthectomy Vesti-bular compensation. 서론 실험방법 1. 실험동물 740
2. 전정기관의회전자극 3. 안구운동의기록및분석 Korean J Otolaryngol 405, May 1997 4. c-fos 면역조직화학법 741
40 5 1997 실험성적 1. 일측전정기관손상후자발안진의변화 2. 일측전정기관손상후회전자극에의한전정안구반사의변화 Fig. 1. Recovery of spontaneous nystagmus following left labyrinthectomy in the same rabbit. F & S, fast and slow component of nystagmus, respectivelycontrol, before labyrinthectomy 5 min, 4 hrs, 12 hrs, 24 hrs are time after labyrinthectomy. Fig. 2. Frequency of spontaneous nystagmus in course of time following unilateral labyrinthectomy. 0 hour means the time at just after labyrinthectomy. The number of rats is 12. Values are meanssd. 742
Korean J Otolaryngol 405, May 1997 Fig. 4. Percent change of slow component velocity of eye movement induced by sinusoidal rotation following unilateral labyrinthectomy. INTACT, rotation toward intact sidelesion, rotation toward lesioned sidec, before labyrinthectomy100%. Negative values are out of phase in eye movement by lesioned side rotation. Fig. 3. Recovery of eye movement induced by sinusoidal rotation at 0.2 Hz following left labyrinthectomy in the same rat. POSITION, position curve; R L, rightward and leftward rotation, respectively. Other notations are as in the previous figures. Fig. 5. Directional preponderance of vestibuloocular reflex induced by sinusoidal rotation following unilateral labyrinthectomy. Maximum value includes more than 100%. Values are meanssd. 743
한이인지 제 4 0 권 제 5 호 1997 Fig. 6. Photographs of c-fos immunoreactive cells in the bilateral medial vestibular nuclei following left labyrinthectomy. CON, before labyrinthectomy; R & L, right and left medial vestibular nuclei, respectively. Asymmetry of c-fos expression in bilateral medial vestibular nuclei is shown at hours 2 and 24 after labyrinthectomy, but symmetry is accomplished at hour 48 after labyrinthectomy. 744
Fig. 7. Number of c-fos immunoreactive cells in the bilateral medial vestibular nuclei following unilateral labyrinthectomy. INTACT, intact side medial vestibular nuclei, LESION, lesioned side medial vestibular nuclei. Values are meanssd. *p0.05, **p0.01. 3. 일측전정기관손상후 c-fos 단백질발현 Korean J Otolaryngol 405, May 1997 고안 745
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