KISEP Experimental Research J Korean Neurosurg Soc 30831-841, 2001 흰쥐에서체감각유발장전위의기록부위별특성과경로분석 * 신현철 ** 박용구 *** 이배환 *** 류재욱 *** 조춘식 *** 정상섭 *** = Abstract = Characteristics and Pathways of the Somatosensory Evoked Field Potentials in the Rat Hyun Chul Shin, M.D.,** Yong Gou Park, M.D.,*** Bae Hwan Lee, Ph.D.,*** Jae Wook Ryou,*** Chun Zhi Zhao,*** Sang Sup Chung, M.D.*** Department of Neurosurgery,** School of Medicine, Donga University, Pusan, Korea Department of Neurosurgery, Medical Research Center, Brain Research Institute,*** Yonsei University College of Medicine, Seoul, Korea bjective - Methods Results- Conclusion - KEY WORDS J Korean Neurosurg SocVolume 30July, 2001 831
서론 - 832 - 재료및방법 1. 실험 1: 흰쥐에서체감각피질의기록위치별 SSEFP의파형변화 - J Korean Neurosurg SocVolume 30July, 2001
1) 실험대상및방법 - - 2. 실험 2: 흰쥐의체감각피질, 시상과박속핵 (Nucleus gracilis) 에서 SSEFP의특성 1) 실험대상및방법 J Korean Neurosurg SocVolume 30July, 2001 833
1. 실험 1 결과 Bregma 1mm 2mm 3mm Lat. 1mm 5mA 4mA 3mA 2mA 1mA 2mm Threshold2.81.1mA 3mm A Stimulus artifact 0.01mV ms Bregma 1mm 2mm 3mm Lat. 1mm 1 2 3 4 5 6 7 8 9 11.633.48 11.463.16.3.26 2mm B 3mm 0.02mV msec.341.86.563.67 8.881.22.292.64 Stimulus artifact 8.923.61 9.580.91 Fig. 1. ARecordings of SSEFP monitored at each sites of cerebral cortex during stimulation of left sciatic nerve. Stimulus intensities were ranged from 1mA to 7mA. When stimulus intensity was increased upto 5mA, response frequency and amplitude increased, but beyond 5mA, they showed no change. SSEFP detection at 4 to 6mm lateral and posterior to bregma was failed. BRecordings of SSEFP monitored at each sites of cerebral cortex in each experimental rats during 5mA stimulation of left sciatic nerve. The vertical line indicate corresponding SSEFP mean onset latency, with standard deviation in, with time in msec. 834 J Korean Neurosurg SocVolume 30July, 2001
- - - - - 4mm Lat 3 2 1 80 80 90 90 80 40 40 30 40 20 30 30 20 20 A Bregma 1 2 3 4 5 6mm Post. 4mm Lat B 3 2 1 40 80 90 80 90 40 80 30 40 30 20 Bregma 1 2 3 4 5 6mm Post. 40 30 20 20 Fig. 2. AAmplitude distribution of P1 wave of SSEFP according to the recording sites. Stimulus intensity was 5mA. The numbers indicate the percentile size of SSEFP compared to maximal amplitude. BSSEFP N1 wave distribution according to the recording sites. Sciatic nerve stimulation with 5mA and the numbers indicate percentile SSEFP. J Korean Neurosurg SocVolume 30July, 2001 835
2. 실험 2 Contralateral Somatosensory Cortex Contralateral Thalamus Ipsilateral Gracile Nucleus 7mA 6mA 5mA A 5V 5V V ms ms ms 1 Contralateral Somatosensory Cortex Contralateral Thalamus Ipsilateral Gracile Nucleus 2 3 4 5 6 7 8 V B V V ms ms ms Fig. 3. ASimultaneously recorded SSEFPs at three different sitescontrolateral cortex, controlateral thalamus, ipsilateral gracile nucleus. The sciatic nerve was stimulated with varing stimulus intensities ranging from 5mA to 7mA. Note the different amplitude scales. BRepresentative stimulus recordings of SSEFPs at three different sites in all experimental rats. Sciatic nerve was stimulated by a single square pulse of 7mA with 0.1msec duration. 836 J Korean Neurosurg SocVolume 30July, 2001
12.28 7.58 N1 N1 N2 P1 N2 8.49 4.4 P1 P2 P2 P3 5V ms A 5V ms B N1 N2 P1 2.9 5V ms P2 C Fig. 4. Schematic diagram of averaging SSEFP wave at controlateral cortexa, contralateral thalamus VPLB, and ipsilateral gracile nucleusc with 7mA stimulus intensity. Table 1. Mean peak latency and mean peak-to-peak amplitude of SSEFP at controlateral cortex when SSEFP were simultaneously recorded at three sitescontrolateral cortex, controlateral thalamus, ipsilateral gracile nucleus. Sciatic nerve was stimulated by a single square pulse of 7mA with 0.1msec duration Latency Onset P1 N1 P2 N2 P3 Meanmsec 7.58 12.38 15.28 19.85 26.67 35.75 S.D. 2.58 2.22 1.9 0.21 6.67 17.12 Amplitude Base-P1 P1-N1 N1-P2 P2-N2 N2-P3 MeanV 4.64 4.47 5.83 6.35 14.74 S.D. 4.02 4.12 3.35 5.12 14.12 S.D.standard deviation 1) 대측체감각피질에서동시에기록한 SSEFP 2) 대측 VPL에서동시에기록한 SSEP Table 2. Mean peak latency and mean peak-to-peak amplitude of SSEFP at controlateral thalamus VPL nucleus when SSEFP were simultaneously recorded at three sitescontrolateral cortex, controlateral thalamus, ipsilateral gracile nucleus. Sciatic nerve was stimulated by a single square pulse of 7mA with 0.1msec duration Latency Onset N1 P1 N2 P2 Meanmsec 4.4 8.49 13.78 19.54 44.55 S.D. 1.9 2.26 2.14 4.84 4.12 Amplitude Base-N1 N1-P1 P1-N2 N2-P2 MeanV 1.36 3.64 2.96 5.51 S.D. 0.69 3.94 2.98 4.82 S.D.standard deviation 3) 동측박속핵에서동시에기록한 SSEP J Korean Neurosurg SocVolume 30July, 2001 837
Table 3. Mean peak latency and mean peak-to-peak amplitude of SSEFP at ipsilateral gracile nucleus when SSEFP were simultaneously recorded at three sitescontrolateral cortex, controlateral thalamus, ipsilateral gracile nucleus. Sciatic nerve was stimulated by a single square pulse of 7mA with 0.1msec duration Latency Onset N1 P1 N2 P2 Meanmsec 2.9 6.6 9.9 12.8 28.7 S.D. 0.5 1.1 1.6 2.1 3.0 Amplitude Base-N1 N1-P1 P1-N2 N2-P2 MeanV 13.64 8.45 4.53 18.34 S.D. 17.26 8.24 4.34 20.16 S.D.standard deviation 4) 동시에기록한체감각피질, VPL, 그리고박속핵의 SSEP 의비교 838 Table 4. Conduction velosities of each sites, onset latency, peak latency of waves and estimated latencymsec according to nucleus gracilis onset latency Cortex OL 1 P1 N1 P2 N2 P3 Latency SD 2 7.58 2.58 12.38 2.22 15.28 1.90 19.85 0.21 26.67 6.67 35.75 17.12 E. Lat 3 11.28 14.58 17.48 33.38 CV 4 25.67 15.72 12.74 9.80 7.30 5.44 VPL 5 OL N1 P1 N2 P2 Latency SD 4.4 1.9 8.49 2.26 13.78 2.14 19.54 4.84 44.55 4.12 E. Lat 8.1 11.4 14.3 30.2 CV 44.02 22.81 14.06 9.91 Nuc. gra. 6 OL N1 P1 N2 P2 Latency SD 2.9 0.5 6.6 1.1 9.9 1.6 12.8 2.1 28.7 3.0 CV 62.07 27.55 18.37 14.06 6.34 1onset latencymsec 2standard deviation 3estimated latencymsec according to nucleus Gracilis onset latency OLcortex or VPLOLNuc. GraNucleus Gracilis peak latency 4conduction velositym/sec Distancecortexstimulus=194.58mm, VPLstimulus= 193.68mm Nucleus Gracilisstimulus = 181.83mm 5ventro posterolateral nucleus of thalamus 6nucleus gracilis J Korean Neurosurg SocVolume 30July, 2001
고찰 - - - J Korean Neurosurg SocVolume 30July, 2001 839
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