1. 2010 6 1, 2 4 Ⅰ,Ⅱ 2013,, -,. - Human Cell.. -,., Biosand Filter(BSF) - BSF Main Filtering (Biofilm) BSF, BSF ( ),.
-, BSF BSF. - BSF BSF ( ),,. BSF -,,,. - BSF,. -. -. -. - 2. BSF -, rrna, BSF.
2. - 5 3 7 (Fig. 1, 2). Fig 1 Fig 2 -,.. - Methanol, MTT assay, 50% (Fig.3) Fig 3 Methanol Human-Cell
1 : Paper Disc Method - Paper Disc,. Spread Plate Culture Pour Plate Culture,, Spreading. 2 : MIC(Minimum Inhibitory Concentration) - Paper Disc MIC Test. MIC. Biosand Filter(BSF) -,,, BSF 93 99% 99.9%. Fig 4 BSF Fig 5 BSF -
. - 3m,. - 2014 KSCY., -. -. BSF, - target.,. BSF, - BSF,.,, BSF., BSF 70% BSF Filtering,, Filtering. - BSF, (Ceramic Filter), (Membrane Filter),, BSF.
BSF, - Biosand Filter, ( 60cm). 10T(1cm). Paper Disc Method,. Paper Disc Clear Zone Steel Ruler. BSF 4 5 6 7 8 9 10 1,300,000 1,243,870 56,130 1,500,000 1,101,300 398,700 1,000,000 1,456,050-456,050 1,700,000 1,699,301 699 5,500,000 5,500,521-521
/, Clean Bench Autoclave Speed Vacuum Concentrator Magnetic bar & Stirrer Biosand Filter & (Escherichia coli) (Staphylococcus aureus) 4 C, Colony,.,,,, LB(Luria Bertan) MSA(Mannitol Salt Agar) (Fig. 6, 7, 8, 9, 10, 11). 2 Biosand Filter(BSF) Table.1 LB(Luria Bertan) (g) Yeast Extract 5 1 Tryptone 10 2 NaCl 10 2 Agar 15 3 - Magnetic Bar, D.W 1l, Stirrer. Yeast Extract, NaCl, Tryptone, Agar 1 : 2 : 2 : 3 1
Mixing., Autoclave 120 C, 1.5 15. 60 C, Petri dish 1/2, 3, 4 C. Table.2 MSA(Mannitol Salt Agar) (g) Phenol Red 0.025 0.01 Beef Extract 2.5 1 Peptone 10 4 Mannitol 10 4 Sodium Chloride 75 30 Agar 15 6 - MSA 100 ml,, ph 7.2 ~ 7.6, Autoclave 120 C, 1.5 15. 60 C, 10%. Petri dish. 10% NaCl, MSA NaCl 9.5%,. Mannitol Acid Phenol Red. Fig 6 Mixing Fig 7 Stirring Fig 8 Autoclave
Fig 9 Fig 10 Fig 11 -,,,. Dring Oven 50 C 3, Ethanol(96.4%), 45 C 72,, 45 C 1. (Fig. 12, 13, 14, 15, 16, 17) Fig 12 Fig 13 Fig 14 Fig 15 Fig 16 Fig 17
: Paper Disc Method - Gram, MSA(Mannitol Salt Agar), Gram, LB(Luria Bertan) 30 μl Spreading,. Paper Disc( 8mm), 50 μl Loading. Ethanol(96.4%) 50 μl, 37 C 24 Incubating, Clear Zone. Clear Zone Disc (8mm) (Fig. 19. 19. 20). Fig 18 Fig 19 Loading Fig 20 Incubating 3. -,, 4 8 Paper Disc Method. - 5, 25 (Table.3). -,. 8
(Fig.21). - 8,,,. Table 3 4 5 6 7 8 4 7 9 14 21 0 5 9 12 21 23 0 5 10 13 22 23 0 (mm) 6 12 13 22 25 0 6 13 15 22 26 0 H2 H3 H3 H5 H5 H0 1.23 2.45 2.95 4.81 5.57 0 - Paper Disc( 8mm) Clear Zone. Ethanol(96.4%). 0~0 H0. 1~5 H1. 5~10 H2. 10~15 H3. 15~20 H4. 20~25 H5. Fig 21 ( )
- 50%, 10%. - 1 10, 20, 30, 40, 50%,.22 30%. 2, 25~35% 1%., 32%, 1% 30% (Ethanol, 96.4%) ( ) (Table.4, Fig.22). 1L 400g. - Paper Disc,.. Table 4 10% 20% 30% 40% 50% 4 5 9 11 12 0 5 6 11 12 13 0 5 6 11 12 13 0 (mm) 6 6 12 12 14 0 6 7 13 14 15 0 H2 H2 H3 H3 H3 H0 1.238 1.422 2.65 2.88 3.08 0
-,, 4 C. - 2, 20(5 ) (Fig.23). - 4 10,. 16 제조기간별항균감수성검사 C l e a r Z o n e 15.25 14.5 13.75 실험군 13 단위 : mm 2 주 4 주 6 주 8 주 10 주 12 주 14 주 16 주 18 주 20 주 제조시기 ( 2 주단위 )
BSF : -, Novosphingobium, Catellibacterium, Aeromonas, Leclercia Microbacterium. 12 5 16S rrna. - BSF 18, Firmicutes Brevibacillus, Bacteroidetes Cloacibacterium, Streptomyces, Microbacterium, Arthrobacter, Sphingomonas, Bradyrhizobium, Klebsiella, Enterobacter, Pantoea. 18 7 16S rrna. - Biosand Filter 9 Bacteroidetes Cloacibacterium, Streptomyces,,Arthrobacter 16S rrna 3 (Fig.24). - Biosand Filter. BSF 생물막의미생물변화 25 18.75 12.5 6.25 0 5 12 189 73 고리울천 BSF 생물막환삼덩굴추출액첨가생물막 미생물 기회성병원균 Fig 24 BSF
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. -,.. -,,.. -. 2014,.,,. Biosand Filter(BSF), -, R&D,.,. -,, HPLC ( ). - Ethanol(94.6%), Water, Methanol.
- 16 rrna,, PCR( ),. 4. & Filter - (2014. 10) : Biosand 2014 : 99 C (2014. 09) 2014 R&E (2014. 11) 2014 (2014.12) (2015. 02) (2015. 03) International Journal of Environmental Research and Public Health (2015. 05) 2015 THE PATHFINDER (2015.9) (2015. 01) Biosand Filter (2015. 01) HPLC, PCR Biosand Filter(BSF)
5. 1, 2010,, 13 2, 2013,, 3, 2008,, ( ) 28 3, 1994,, 26 4, 2008,, 28 5, 2012, in vitro, 25 2, 2002,,, 6, 2014, (Humulus japonicus), 46, 2006, Ashobolt N.J, 2004, microbial contamination of drinking water and disease outcomes in developing regions. Toxicology, p. 229 238. Sianipar C.P.M.; Yudoko G.; Dowaki K.; Adhiutama A, 2005, Design methodology for appropriate technology: Engineering as if people mattered. Sustainability p. 3382 3425 Clifford M.J, 2005, Appropriate technology: The poetry of science. Sci. Christ. Belief, p. 71 82. Stauber C.E.; Elliott M.A.; Ortiz G.M.; DiGiano F.A.; Sobsey M.D. 2006, Charactersation of The Biosand Filter for E. coli Reductions from Housegold Drinking Water under Controlled Laboratory and Filed Use Conditions. Water Science & Technology, p. 1 7.