한수지 49(5), , 2016 Original Article Korean J Fish Aquat Sci 49(5), ,2016 여름철참돔 (Pagrus major) 의절식에따른생존과생리적반응 이정용 * 이정환 1 허준욱 1 국립수산과학원양식관리

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1 한수지 49(5), , 2016 Originl Article Koren J Fish Aqut Sci 49(5), ,2016 여름철참돔 (Pgrus mjor) 의절식에따른생존과생리적반응 이정용 * 이정환 1 허준욱 1 국립수산과학원양식관리과, 1 ( 주 ) 생물모니터링센터 Effect of Strvtion on Survivl nd Physiologicl Response in Red Se Brem Pgrus mjor in Summer Jeong Yong Lee*, Jung Hwn Lee 1 nd Jun Wook Hur 1 Aquculture Mngement Division, Ntionl Institute of Fisheries Science, Busn 46083, Kore 1 Bio-Monitoring Center, Dejeon 34576, Kore A 60-dy experiment ws conducted to determine the effect of feeding nd strvtion on the survivl, growth, nd blood prmeters of red se brem Pgrus mjor. The strved group ws not fed during the first 32 dys of the experiment nd ws then fed for 28 dys. The growth rte of the strved fish group ws significntly lower thn tht of the fed fish group. Strvtion resulted in growth retrdtion nd reductions in finl body weight, growth rte, specific growth rte, nd condition fctor, wheres the fed fish group grew well nd mintined good condition. The growth rte of the strved group tht ws refed ws higher thn tht of the fed group. Red blood cells, hemtocrit, nd hemoglobin showed no significnt differences between the fed nd strved groups. The cortisol nd glucose levels of the fed group of juveniles were higher thn those of the strved group. The cortisol levels of the strved group of dults were higher thn those of the fed group, wheres the glucose levels of the strved group were lower thn those of the fed group. These results suggest tht the survivl, growth, nd hemtologicl prmeters used to reflect strvtion nd feeding in this study provide useful index of physiologicl response nd survivl rte in red se brem. Key words: Red se brem, Strvtion, Survivl, Growth, Blood prmeter 서론 (wintering), (spwning migrtion) (strvtion), (Love, 1970; Wetherley nd Gill, 1987).,, (endogenous), (degrowth), (Wetherley nd Gill, 1987; Prk, 2004). (Oncorhynchus mykiss) (Prlichthys olivceus),,,,,, (Wetherley nd Gill, 1981; Woo, 2005)., glucose Atlntic cod (Gdus morhu), Europen eel (Anguill nguill), (Esox lucius) (Ince nd Thorpe, 1976; Lrsson nd Lewnder, 1973; Krm, 1966), (Opsnus tu), goldfish (Crssins urtus) Americn eel Anguill rostrt (Tshim nd Chill, 1968; Chvin nd Young, 1970; Moon, 1983). red blood cell (RBC), hemtocrit (Ht), hemoglobin (Hb), glucose, cortisol (N +, Cl -, K + ) (Woo, 2005). Koren J Fish Aqut Sci 49(5) , October 2016 This is n Open Access rticle distributed under the terms of the Cretive Commons Attribution Non-Commercil Licens ( which permits unrestricted non-commercil use, distribution, nd reproduction in ny medium, provided the originl work is properly cited. Received 26 My 2016; Revised 9 September 2016; Accepted 21 September 2016 *Corresponding uthor: Tel: Fx: E-mil ddress: ljy1968@kore.kr Copyright 2016 The Koren Society of Fisheries nd Aqutic Science 620 pissn: , eissn:

2 여름철참돔의절식에따른생존과생리적반응 621 Europen eel, Americn eel (Lrsson nd Lewnder, 1973; Moon, 1983), O. mykiss E. lucius (Wetherley nd Gill, 1981; Ince nd Thorpe, 1976) O. tu (Tshim nd Chill, 1968). O. mykiss E. lucius (Kwtsu, 1966; Ince nd Thorpe, 1976) Ht, Love (1970) Ht. Lrsson nd Lewnder (1973) Europen eel Ht. 25,. 실험어류 재료및방법 (Pgrus mjor) , 2. digitl vernier cliper (CD-20CP, Jpn) (JW-1, Kore), 0.1 cm 0.1 g. 실험조건및사육관리 3 ( cm) 100 (fed group) (strved group), cm , dissolved oxygen mg/l ph crude protein 50%, crude ft 10%, crude fiber 5%, sh 18%, clcium 1% phosphorus 2.7% (Cj CheilJedng Corp., Kore). 2 (9:00 18:00).., 4, 8, 생존율및성장분석., 10, digitl vernier cliper 0.1 cm 0.1 g (growth rte for totl length, GRL) (growth rte for body weight, GRW) (%)=( ) 100/, (%)=( ) 100/. (specific growth rte, %) =( )/, (condition fctor) = 100/ 3. (feed intke) = /( ), (specific feeding rte) (%)=( 100)/[( + + )/2], (feed conversion rte, %) =( 100)/ ). 혈액채취및성분분석 4, 8, 16, IU/mL heprin sodium (Choongwe Phrmcy Corportion, Kore) (23 G) 1. RBC, Ht, Hb, (14,000 g, 4, 10 ) -80. RBC, Ht, Hb Auto Hemtology Anlyzer BC-2800 Vet (MINDRAY, Chin). cortisol Donldson (1981) Cot-A-Count TKCO Cortisol RIA Kit (DPC, USA), 1470 WIZ- ARD Automtic Gmm Counter (Cobr, Pckrd Co., USA) rdioimmunossy (RIA)

3 622 이정용ㆍ이정환ㆍ허준욱 Survivl (%) Glucose (Johnson & Johnson Medicl Kore Ltd., Kore), N +, K + Cl - electrolyte nlyzer i-smrt 30 vet (i-sens Inc., Kore). 통계처리 SPSS- (SPSS 18.0, SPSS Inc., USA) ANOVA Duncn's multiple rnge test. 결과 Juvenile fed Juvenile Strved Adult fed Adult strved Rering dys Fig. 1.Survivl rte of red se brem Pgrus mjor fed nd strved for 60 dys. Strved groups were strved for 32 dys nd then refed until 60 dys. 생존율및성장분석 %,, % %. 100% (Fig. 1). 32 Tle 1. Tle 1. The chnge of growth of red se brem Pgrus mjor fed nd strved for 60 dys TL (mm) BW (g) GRL (%) GRW (%) Items Specific growth rte (%) Condition fctor Feed intke (%) Specific feeding rte (%) Rering dys Juvenile Adult Fed Strved Fed strved ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ±0.6 - Feed conversion rte (%) ± ± ± ±1.1 The vlues re men±sd (Fed: n=10, Strved: n=10). Strved groups were strved for 32 dys nd then refed until 60 dys. TL, Totl length; BW, Body weight; GRL, Growth rte for totl length; GRW, Growth rte for body weight.

4 여름철참돔의절식에따른생존과생리적반응 Initil Fed Strved (A) d Initil Fed Strved (B) Cortisol (ng/ml) b c Cortisol (ng/ml) bc c c b Initil Fed Strved e (C) Initil Fed Strved (D) f Glucose (mg/dl) bc cd cd cd d bcd cb bc Glucose (mg/dl) bcd c de bcd cd e de Rering dys Rering dys Fig. 2.Vritions of cortisol nd glucose levels in plsm of fed nd strved groups in red se brem Pgrus mjor for 60 dys (n=10). Strved groups were strved for 32 dys nd then refed until 60 dys. Different lphetic letters on the brs mens significntly different (P>0.05). (A): dult red se brem, (B) : juvenile red se brem, (C) : dult red se brem, (D) : juvenile red se brem mm ( mm) 1.3, mm, mm mm. 32, g g ( g, g). (, ) 32, , , (60 ), mm ( mm) 1.1, mm, mm mm. 32, g g ( g, g). 32, ( ), 60 ( ) (60 ) ( ), ( ). 혈액 prmeter 변화 Cortisol ng/ml, ( ng/ml) ( ng/ml) 60 (Fig. 2A) ng/ml, 32 ( ng/ml), 4 ( ng/ml)

5 624 이정용ㆍ이정환ㆍ허준욱 (Fig. 2B). Glucose mg/dl, mg/ dl mg/dl (Fig. 2C) mg/dl, 32 ( mg/dl), mg/dl (Fig. 2D). RBC, Hb, Ht (N +, K +, Cl - ) Tle 2 Tle 3. RBC cell/ L, cell/ L cell/ L (P 0.05) cell/ L, cell/ L cell/ L (P 0.05). Hb g/dl, g/dl g/dl g/dl, 32 Tle 2. Chnges of red blood cell, hemoglobin, hemtocrit of red se brem Pgrus mjor fed nd strved for 60 dys Group Juvenile Adult Rering Red blood cell ( 10 6 cell/μl) Hemoglobin (g/dl) Hemtocrit (%) Dys Fed Strved Fed Strved Fed Strved 0 1.7± ± ± ± ± ± ± ± ± ±0.1 c 22.1± ± ± ±0.9 bc 4.1± ± ± ± ± ±0.5 bc 4.5± ±0.1 bc 30.4± ± ±0.6 b 2.8±0.5 bc 7.4±1.5 b 6.9±0.1 c 37.3±10.0 b 27.3± ±0.8 c 3.2±0.6 c 10.1±2.2 c 8.7±0.1 d 48.2±9.8 c 39.0±10.8 b 0 3.5±0.6 c 3.5±0.6 c 11.1±1.2 c 11.1±0.1 c 47.9±3.7 b 47.9±3.7 bc 4 3.2±0.3 bc 2.7± ± ± ± ± ± ±0.4 b 8.0± ±0.1 bc 39.4± ±8.5 c ±0.5 bc 3.0± ±0.8 bc 8.9±0.1 b 46.8±4.0 b 41.3±7.5 c ± ± ± ± ± ± ±0.6 bc 2.8± ±0.6 bc 9.7±0.1 bc 40.8± ±2.8 bc The vlues re men±sd (Fed: n=10, strved: n=10). Strved groups were strved for 32 dys nd then refed until 60 dys. Vlues (men±sd) in the sme row men not shring common superscript re significntly different (P<0.05). Tle 3. Chnges of N +, K + nd Cl - of red se brem Pgrus mjor fed nd strved for 60 dys Group Juvenile Adult Rering N + (mmol/l) K + (mmol/l) Cl - (mmol/l) Dys Fed Strved Fed Strved Fed Strved ± ± ±0.2 cd 7.9±0.2 b 120.0± ± ± ± ± ±1.7 b 126.8± ± ± ±13.7 bc 5.9±1.5 bc 6.2± ±8.7 bc 135.6± ±16.1 bc 149.2± ±2.8 d 8.2±3.6 b 136.7±10.3 bc 126.9± ±13.3 cd 150.9± ±2.5 cd 8.6±2.3 b 149.5±11.0 cd 132.7± ±7.6 d 177.3±5.9 c 3.1± ±1.0 b 155.4±3.9 d 145.0±9.2 b ±2.7 bc 187.6±2.7 b 0.6± ± ± ± ±7.1 cd 189.1±12.9 b 0.6± ± ± ± ±9.7 b 160.8± ± ± ± ±2.2 c ±6.0 bcd 192.2±4.8 b 0.8± ± ± ±4.4 bc ±9.5 d 185.5±5.1 b 0.6± ± ± ±5.0 bc ± ±4.6 c 2.1±0.8 b 2.1±0.8 b 157.6± ±3.6 c The vlues re men±sd (Fed: n=10, strved: n=10). Strved groups were strved for 32 dys nd then refed until 60 dys. Vlues (men±sd) in the sme row men not shring common superscript re significntly different (P<0.05).

6 여름철참돔의절식에따른생존과생리적반응 g/dl g/dl. 60 Hb g/ dl g/dl. Ht %, %, %. 60 Ht % % (P 0.05). Ht % % (P 0.05). N mmol/l mmol/l, mmol/l, mmol/l mmol/l. N mmol/l mmol/l, mmol/l. K mmol/l, 16, mmol/l, mmol/l. Cl mmol/l, mmol/l, mmol/l mmol/l, mmol/l. 고찰,., (Wetherley nd Gill, 1981; Lee et l., 1998; Prk et l., 1998; Prk et l., 2001). 94%. 4 (Prk et l., 2006) 9 (Prk et l., 2002) 93.8% 96.4%, 80 (Lee et l., 2008) 80.2% 32., % %., Slmo girdneri % 32.5% (Wetherley nd Gill, 1981), 32,, (Sumpteret l., 1991). 32,, (Seo et l., 2009) , 60., Ictlurus puncttus (Gylord nd Grtlin, 2000), Crssius urtus (Xie et l., 2001) hybrid tilpi (Wng et l., 2000). Cortisol, glucose, RBC, Ht, Hb (Brton et l., 1985; Thoms nd Robertson, 1991). Cortisol Glucose (Brton nd Iwm, 1991). Bisws et l. (2006) cortisol glucose 7-15 ng/ml mg/dl., 0, 4, ng/ml. cortisol. cortisol cortisol, corticotropin-relesing hormone (Pepels et l., 2004). Glucose mg/dl 90 mg/dl 32. glucose cortisol gluconeogenesis cortisol

7 626 이정용ㆍ이정환ㆍ허준욱 (Pickering nd Pottinger, 1989), Brton nd Iwm (1991) stress glucose cortisol. 32 cortisol glucose, cortisol glucose. stress. Ht, RBC Hb, (Dvis nd Prker, 1990). Jung et l. (2006) Ht Hb 40-46% g/dl. Ht Hb,, 32 Ht, RBC Hb. N +,, K + Cl -. 32, (Myeong et l., 2011). 32,.. 사사 2016 (R ),. References Brton BA, Weiner GS nd Schreck CB Effect of prior cid exposure on physiologicl responses of juvenile rinbow trout (Slmo girdneri) to cute hndling stress. Cn J Fish Aqut Sci 42, Brton BA nd Iwm GK Physiologicl chnges in fish from stress in quculture with emphsis on the response nd effects of corticosteroids. Annu Rew Fish Dis 1, Bisws AK, Seok M, Tkii K, Mit M nd Kumi H Stress response of red se brem Pgrus mjor to cute hndling nd chronic photoperiod mnipultion. Aquculture 252, Chvin W nd Young JE Fctors in the determintion of norml serum glucose levels of goldfish, Crssius urtus L. Comp Biochem Physiol 33, org/ / x(70) Dvis KB nd Prker NC Physiologicl stress in striped bss: Effect of cclimtion temperture. Aquculture 91, Donldson EM The pituitry interrenl xis s n indictor of stress in fish. In: Stress nd Fish. Pickering AD, ed. Acdemic Press, New York, U.S.A., Gylord TG nd Gtlin DM Assessment of compenstory growth in chnnel ctfish (Ictlurus puncttus) nd ssocited chnges in body condition indices. J World Aqucult Soc 31, tb00884.x. Ince BW nd Thorpe A The effects of strvtion nd force-feeding on the metolism of the northern pike, Esox lucius L. J Fish Biol 8, org/ /j tb03909.x. Jung SH, Byun SG, Jee BY nd Choi HS Appliction of veterinry chemistry nlyzer used to hemtologicl nlysis of mrine fish cultured in floting netcge. J Fish Pthol 19, Krm SK Effects of strvtion nd refeeding on some liver nd blood constituents of Atlntic cod (Gdus morhu L.). J Fish Res Bd Cn 23, org/ /f Kwtsu H Studies on the nemi of fish. 1. Anemi of rinbow trout cused by strvtion. Bull Freshwter Fish Res L Tokyo 15, Lrsson A nd Lewnder K Metolic effects of strvtion in the eel, Anguill nguill L. Comp Biochem Physiol 44, Lee CK, Prk IS nd Hur SB Influence of strvtion on the vritions of heptocyte nucleus in lrve of red spotted grouper, Epinephelu skr. J Aqu 11, Lee JY, Hur JW nd Kim SG Effects of strvtion on growth nd physiologicl response in cultured ctfish, Silurus sotus. Koren J Ichthyol 20, Love RM The Chemicl Biology of Fishes with Key to the Chemicl Literture, Acdemic Press, London, U. K., Moon TW Metolic reserves nd enzyme ctivities with food deprivtion in immture Americn eels, Anguill rostrt (Lesueur). Cn J Zool 61, org/ /z Myeong JI, Kng DK, Kim HC, Lee JH, Noh JK nd Kim HC Chnges of stress response nd physiologicl metolic ctivity of flounder, Prlichthys olivceus following

8 여름철참돔의절식에따른생존과생리적반응 627 to food deprivtion nd slow temperture descending. Kor J Ichthyol 23, Prk IS, Lee CK, Lim JH, Kim JH nd Kim SU Effect of strvtion on the growth nd heptocyte nucler size of lrvl rockfish Sebste sschlegeli nd lrvl spotted se bss Lteolrx sp. J Aqucult 11, Prk IS, Im JM, Ryu DK, Nm YK nd Kim DS Effect of strvtion on morphmetric chnges in Rhynchocypris oxycephlus (Suvge nd Dry). J Appl Ichthyol 17, Prk IS, Im JH, Jeong CH, Noh JK, Kim YH nd Lee YH Effect of Strvtion on Some Nutritionl Prmeters in Rhynchocypris oxycephlus (Suvge nd Dry): Chrcteristics of the Morphometric Chnges in the Sectioned Body. J Appl Ichthyol 14, Prk IS Effect of strvtion on some prmeters in Rhynchocypris oxycephlus (Suvge nd Dry): A Review. Koren J Environ biol 22, Prk IS, Woo SR, Kim EM nd Cho SH Effect of feeding nd strvtion on growth nd phenotypic trit in olive flounder, Prlichthys olivceus (Temmincket Schlegel). J Aqucult 19, Pepels PPLM, Wendelr SE nd Blm PHM Bcteril lipopolyscchride (LPS) modultes corticotrophin-relesing hormone (CRH) content nd relese in the brin of juvenile nd dult tilpi (Oreochromis mossmbicus; Teleostei). J Exp Biolol 207, jep Pickering AD nd PottingerTG Stress responses nd disese resistnce in slmonid fish: Effects of chronic elevtion of plsm cortisol. Fish Physiol Biochem 7, dx.doi.org/ /bf Seo JY, Kim KD nd Lee SM Effects of supplementl herb medicines in the diets on growth, feed utiliztion nd body composition of juvenile nd grower rockfish, Sebstes schlegeli. J Aqucult 22, Sumpter JP, Le Bil PY, Pickering AD, Pottinger TG nd Crrgher JF The effect of strvtion on growth nd plsm growth hormone concentrtions of rinbow trout, Oncorhynchus mykiss. Gen Comp Endocrinol 83, Tshim L nd Chill CF Effects of insulin in the todfish, Opsnus tu. Gen Comp Endocrinol 11, dx.doi.org/ / (68) Thoms P nd Robertson L Plsm cortisol nd glucose stress responses of red drum (Scienops ocelltus) to hndling nd shllow wter stressors nd nesthesi with MS- 222, quildine sulfte nd metomdte. Aquculture 96, Wng Y, Cui Y, Yng Y nd Ci F Compenstory growth in hybrid tilpi, Oreochromis mossmbicus O. niloticus, rered in sewter. Aquculture 189, Wetherley AH nd Gill HS Recovery growth of following periods or restricted rtions nd strvtion in rinbow trout, Slmo girdneri Richrdson. J Fish Biol 18, Wetherley AH nd Gill HS The biology of fish growth. 4. Protein, lipid nd cloric contents. Acdemic press, London, U. K., Woo SR, Effect of strvtion on prmeters in olive flounder, Prlichthys olivceus (Temmincket Schlegel). MS Thesis, Kore Mritime University, Busn, Kore. Xie S, Xhu Y, Cui Y, Wootton RJ, Lei W nd Yng Y Compenstory growth in the gibel crp following feed deprivtion: temporl ptterns in growth, nutrient deposition, feed intke nd body composition. J Fish Biol 8,

Kor J Fish Aquat Sci 44(6), DOI : 44(6), , 2011 순치기간에따른육봉형산천어 (Oncorhynchus masou masou) 의해수적

Kor J Fish Aquat Sci 44(6), DOI : 44(6), , 2011 순치기간에따른육봉형산천어 (Oncorhynchus masou masou) 의해수적 Kor J Fish Aqut Sci 44(6), 753-758 DOI : http://dx.doi.org/.5657/kfas.211.753, 44(6), 753-758, 211 순치기간에따른육봉형산천어 (Oncorhynchus msou msou) 의해수적응능력 김병기ㆍ김재원 ㆍ박정환 1 ㆍ성기백 2 ㆍ김현주 3 강원도립대학해양생명과학과, 1 노스캐롤라이나주립대학생물학과,