한수지 50(6), , 2017 Original Article Korean J Fish Aquat Sci 50(6), ,2017 암모니아급성노출에의한바이오플락사육넙치 (Paralichthys olivaceus) 의혈액학적성상및항산화반응의변화 김준

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1 한수지 5(), , 17 Originl Article Koren J Fish Aqut Sci 5(),75-755,17 암모니아급성노출에의한바이오플락사육넙치 (Prlichthys olivceus) 의혈액학적성상및항산화반응의변화 김준환 * 김기욱 배선혜 김수경 김수경 김종현 국립수산과학원서해수산연구소 Altertions in Hemtologicl Prmeters nd Antioxidnt Responses in the Biofloc-rered Fltfish Prlichthys olivceus Following Ammoni Exposure Jun-Hwn Kim*, Ki Wook Kim, Sun-Hye Be, Su-Kyoung Kim, Su Kyoung Kim nd Jong-Hyun Kim West Se Fisheries Reserch Institute, Ntionl Institute of Fisheries Science, Ten 313, Kore Fltfish Prlichthys olivceus rised in iofloc system (men length 7.±3.1 cm, men weight 8.±.5 g) were exposed for to different concentrtions of mmoni (, 8, 1, 3, nd mg NH + /L) for 7 dys. Following mmoni exposure, hemtologicl prmeters such s hemogloin nd hemtocrit were significntly reduced. Plsm components such s mgnesium, glucose, sprtte minotrnsferse (AST), nd lnine minotrnsminse (ALT) were significntly ltered y mmoni exposure, wheres there were no significnt chnges in clcium, cholesterol, or totl protein. Antioxidnt responses, such s superoxide dismutse (SOD) nd ctlse (CAT) levels were significntly elevted following mmoni exposure. The results of this study indicte tht mmoni exposure induces significnt chnges in hemtologicl prmeters nd ntioxidnt responses in iofloc-rered Prlichthys olivceus s toxic response. Key words: Prlichthys olivceus, Ammoni, Hemtologicl prmeter, Antioxidnt response, Biofloc 서론, (Rndll nd Tsui, ; Kim et l., 17)., -8% (Eddy, 5).,.,,,, (Kim et l., 15). (NH 3 ),, (NH + )., (NH + ), N + -K + ATPse N + /K + /Cl - (K + ),,, (Roumieh et l., 13)., N-methyl-D-sprtte (NMDA), C + N +, C + C + -, (Rndll nd Tsui, ). (Kim nd Kng, 1)., (hemogloin), (Ds et l., )., (hemogloin) (hemtocrit) Koren J Fish Aqut Sci 5() , Decemer 17 This is n Open Access rticle distriuted under the terms of the Cretive Commons Attriution Non-Commercil Licens ( which permits unrestricted non-commercil use, distriution, nd reproduction in ny medium, provided the originl work is properly cited. Received 8 Octoer 17; Revised 5 Octoer 17; Accepted 1 Novemer 17 *Corresponding uthor: Tel: Fx: E-mil ddress: junhwn198@kore.kr Copyright 17 The Koren Society of Fisheries nd Aqutic Science 75 pissn: , eissn:

2 바이오플락사육넙치의암모니아독성 751. (Kim nd Kng, 1).. N-Methyl-D-sprtic cid (NMDA), nitric oxide (NO), rective oxygen species (ROS) rective nitrogen speices (RNS) (Norenerg et l., 7; Hegzi et l., ).., (Dy et l., 1).,...,,. 재료및방법 실험어및실험환경 g, Tle 1. The chemicl components of sewter nd experimentl condition used in the experiments Item Vlue Temperture ( C).±.5 ph 8.9±. Slinity (PSU) 33.8±.5 Dissolved oxygen (mg/l) 7.5±.1 ( cm, 8..5 g).,,. NH Cl (Sigm Chemicl, St. Louis, MO, 99.%),, mg NH + /L,, 8, 1, 3 mg NH + /L. 15 L 5 5, Tle 1-.,, 3.,,,., ()., ethyl-3-minoenzote methnesulfonte slt. 혈액학적성상및혈장성분 1 ml,. hemogloin kit (Asn Phrm. Co., Ltd) Cynmethemogloin, hemtocrit, Microhemtocrit centrifuge (VS-1, ) 1, rpm, Micro-hemtocrit reder. C, rpm,. clcium mgnesium. Clcium OCPC, mgnesium Xylidyl lue-i kit (Asn Phrm. Co., Ltd). glucose, cholesterol, totl protein. Glucose GOD/POD, cholesterol enzyme method, totl protein Biuret kit (Asn Phrm. Co., Ltd). AST (Asprtte minotrnsferse), ALT (Alnine minotrnsminse), ALP (Alkline phosphtse). AST ALT 55 nm Reitmn- Frnkel, ALP King-King 5 nm kit (Asn Phrm. Co., Ltd). Tle. Anlyzed mmoni concentrtions (mg/l) from ech source Exposure concentrtion (mg/l) Nominl mmoni concentrtions Mesured mmoni concentrtions.38± ± ± ± ±3.71

3 75 김준환ㆍ김기욱ㆍ배선혜ㆍ김수경ㆍ김수경ㆍ김종현 항산화반응 superoxide dismutse (SOD) ctlse (CAT).,,.1 M PBS (phosphte uffer solution) PBS (.1 M phosphte uffer solution). C, rpm 3. SOD WST-1 5% SOD ssy kit (Sigm Chemicl, St. Louis, MO) (Kim nd Kng, 15). CAT CAT ssy kit (Sigm Chemicl, St. Louis, MO) (Kim nd Kng, 17). ovine plsm lumin (Sigm Chemicl, St. Louis, MO) Brdford s method (197). 통계분석방법 SPSS (SPSS Inc.) ANOVA test, Duncn s multiple rnge test P<.5. mg NH + /L % (1 ). 결과 혈액학적성상및혈장성분 Fig. 1. hemogloin hemtocrit 3 mg NH + / L (P<.5). clcium Hemogloin (g/dl) mg/l 3 mg/l mg/l Hemtocrit (%) mg/l 3 mg/l mg/l Clcium (mg/dl) 8 1 mg/l 3 mg/l mg/l Blood Blood Plsm Mgnesium (mg/dl) mg/l 3 mg/l mg/l Glucose (mg/dl) 8 1 mg/l 3 mg/l mg/l Cholesterol (mg/dl) mg/l 3 mg/l mg/l Plsm Plsm Plsm Totl Protein (g/dl) 8 1 mg/l 3 mg/l mg/l AST (Krmen unit) 8 1 mg/l 3 mg/l mg/l ALT (Krmen unit) 8 1 mg/l 3 mg/l mg/l Plsm Plsm Plsm Fig. 1. Hemtologicl prmeters (hemtocrit, hemogloin, clcium, mgnesium, glucose, cholesterol, totl protein, AST nd ALT) of fltfish Prlichthys olivceus exposed to mmoni for 7 dys. Vlues with different superscript re significntly different (P<.5) s determined y Duncn's multiple rnge test. AST, sprtte minotrnsferse; ALT, lnine minotrnsminse.

4 바이오플락사육넙치의암모니아독성 753, mgnesium 3 mg NH + /L (P<.5). glucose 3 mg NH + /L (P<.5)., cholesterol totl protein. AST 3 mg NH + /L, ALT 8 mg NH + /L (P<.5). 항산화반응 SOD CAT Fig.. SOD 1 mg NH + /L (P<.5). SOD 3 mg NH + /L (P<.5). CAT 1 mg NH + /L (P<.5), CAT 3 mg NH + /L (P<.5). 고찰 9% (Rm nd Mnjht, 1).,, (Kim et l., 17)., fltfish Prlichthys olivceus hemogloin hemtocrit,. Ds et l. () mrigl Cirrhinus mrigl hemogloin, (hemodilution). clcium mgnesium, (Kim nd Kng, 1)., SOD (Unit/mg protein) mg/l 3 mg/l mg/l SOD (Unit/mg protein) 8 1 mg/l 3 mg/l mg/l Liver Gill CAT (um /min/mg protein) mg/l 3 mg/l mg/l CAT (um /min/mg protein) 8 1 mg/l 3 mg/l mg/l Liver Gill Fig.. Antioxidnt responses (SOD nd CAT ctivity) of fltfish Prlichthys olivceus exposed to mmoni for 7 dys. Vlues with different superscript re significntly different (P<.5) s determined y Duncn's multiple rnge test. SOD, superoxide dismutse; CAT, ctlse.

5 75 김준환ㆍ김기욱ㆍ배선혜ㆍ김수경ㆍ김수경ㆍ김종현 P. olivceus mgnesium,. Kim et l. (17) slefish Anoplopom fimri clcium mgnesium. glucose (Kim nd Kng, 1; Kim nd Kng, 15; Kim nd Kng, 1). glucose ctecholmine drenline nor-drenline, ctecholmine glycogen glucose., P. olivceus glucose. cholesterol,, (Firt nd Krgin, )., P. olivceus cholesterol. Ds et l. () mirgl Cirrhinus mrigl protein, totl protein. AST (sprtte minotrnsferse) ALT (lnine minotrnsminse), (Lemire et l., 1991; Rm nd Mnjht, 1). P. olivceus AST ALT. crp Cyprinus crpio; lck se rem Acnthopgrus schlegelii; slefish Anoplopom fimri AST ALT (Jeney et l., 199; Kwon nd Chng, 199; Kim et l., 17),., (Kosenko et l., 1997). SOD (superoxide dismutse) 1 superoxide rdicl (H O ) (O ). CAT (ctlse) (H O ).,, SOD CAT (Rm nd Mnjht, 1). P. olivceus SOD CAT. Cheng et l. (15) pufferfish Tkifugu oscurus rective oxygen species (ROS). Kim et l. (15) rockfish Sestes schlegelii SOD CAT. ROS, ROS,. ROS (Kim nd Kng, 17; Kim et l., 17c). P. olivceus. P. olivceus 3 mg NH + /L, 1 mg NH + /L... 사사 17 (BFT) (R171).,,. References Brdford MM A rpid nd sensitive method for the quntittion of microgrm quntities of protein utilizing the principle of protein-dye inding. Anl Biochem 7, Cheng CH, Yng FF, Ling RZ, Lio SA, Mio YT, Ye CX nd Wng AL. 15. Effects of mmoni exposure on poptosis, oxidtive stress nd immune response in pufferfish (Tkifugu oscurus). Aqut Toxicol 1, org/.1/j.qutox Ds PC, Ayyppn S, Jen JK nd Ds BK.. Acute toxicity of mmoni nd its su-lethl effects on selected hemtologicl nd enzymtic prmeters of mrigl, Cirrhinus mrigl (Hmilton). Aqucult Res 35, org/.1111/j x. Dy SB, Slie K nd Stnder HB. 1. A growth comprison mong three commercil tilpi species in iofloc system. Aqucult Int, s z. Eddy FB. 5. Ammoni in esturies nd effects on fish. J

6 바이오플락사육넙치의암모니아독성 755 Fish Biol 7, Firt O nd Krgin F.. Individul nd comined effects of hevy metls on serum iochemistry of Nile tilpi Oreochromis niloticus. Arch Environ Contm Toxicol 58, Hegzi MM, Atti ZI nd Ashour OA.. Oxidtive stress nd ntioxidnt enzymes in liver nd white muscle of Nile tilpi juvenile in chronic mmoni exposure. Aqut Toxicol 99, Jeney G, Nemcsok J, Jeney Z nd Olh J Acute effect of sulethl mmoni concentrtions on common crp (Cyprinus crpio L.). II. Effect of mmoni on lood plsm trsminses (GOT, GPT), G1DH enzyme ctivity, nd ATP vlue. Aquculture, org/.1/-88(9)915-b. Kim JH nd Kng JC. 1. The selenium ccumultion nd its effect on growth, nd hemtologicl prmeters in red se rem, Pgrus mjor, exposed to wterorne selenium. Ecotoxicol Environ Sf, ecoenv.1... Kim JH nd Kng JC. 15. Oxidtive stress, neurotoxicity, nd non-specific immune responses in juvenile red se rem, Pgrus mjor, exposed to different wterorne selenium concentrtions. Chemosphere 135, dx.doi.org/.1/j.chemosphere Kim JH nd Kng JC. 15. The led ccumultion nd hemtologicl findings in juvenile rock fish Sestes schlegelii exposed to the dietry led (II) concentrtions. Ecotoxicol Environ Sf 115, ecoenv Kim SH, Kim JH, Prk MA, Hwng SD nd Kng JC. 15. The toxic effects of mmoni exposure on ntioxidnt nd immune responses in Rockfish, Sestes schlegelii during therml stress. Environ Toxicol Phrmcol, Kim JH nd Kng JC. 1. Oxidtive stress, neurotoxicity, nd metllothionein (MT) gene expression in juvenile rock fish Sestes schlegelii under the different levels of dietry chromium (Cr + ) exposure. Ecotoxicol Environ Sf 15, Kim JH nd Kng JC. 1. The chromium ccumultion nd its physiologicl effects in juvenile rockfish, Sestes schlegelii, exposed to different levels of dietry chromium (Cr + ) concentrtions. Environ Toxicol Phrmcol 1, Kim JH nd Kng JC. 17. Growth performnce, oxidtive stress, nd non-specific immune responses in juvenile slefish, Anoplopom fimri, y chnges of wter temperture nd slinity. Fish Physiol Biochem 3, doi.org/.7/s z. Kim JH nd Kng JC. 17. Effects of su-chronic exposure to led (P) nd scoric cid in juvenile rockfish: Antioxidnt responses, MT gene expression, nd neurotrnsmitters. Chemosphere 171, Kim JH, Prk HJ, Hwng IK, Hn JM, Kim DH, Oh CW, Lee JS nd Kng JC. 17. Toxic effects of juvenile slefish, Anoplopom fimri y mmoni exposure t different wter temperture. Environ Toxicol Phrmcol 5, Kim JH, Prk HJ, Hwng IK, Hn JM, Kim DH, Oh CW, Lee JS nd Kng JC. 17. Altertions of growth performnce, hemtologicl prmeters, nd plsm constituents in the slefish, Anoplopom fimri depending on mmoni concentrtions. Fish Aqut Sci, :. s Kim JH, Oh CW md Kng JC. 17c. Antioxidnt responses, neurotoxicity, nd metllothionein gene expression in juvenile Koren rockfish Sestes schlegelii under dietry led exposure. J Aqut Anim Helth 7, g/.8/ Kosenko E, Kminsky M, Kminsky A, Vlenci M, Lee L, Hermenegildo C nd Felipo V Superoxide production nd ntioxidnt enzymes in mmoni intoxiction in rts. Free Rdicl Res 7, / Kwon JY nd Chng YJ Effects of mmoni concentrtion on histologicl nd physiologicl sttus in lck serem (Acnthopgrus schlegeli). Koren J Fish Aqut Sci 9, Lemire P, Dri P, Mthieu A, Lemire S, Crriere S, Giudicelli J nd Lfurie M Chnges with different diets in plsm enzymes (GOT, GPT, LDH, ALP) nd plsm lipids (cholesterol, triglycerides) of se-ss (Dicentrrchus lrx). Aquculture 93, org/.1/-88(91)95-l. Norenerg MD, Jykumr AR, Ro KVR nd Pnickr KS. 7. New concepts in the mechnism of mmoni-induced strocyte swelling. Met Brin Dis, org/.7/s Rm S, Mnjht SN. 1. Protective effect of shrimp crotenoids ginst mmoni stress in common crp, Cyprinus crpio. Ecotoxicol Environ Sf 7, org/.1/j.ecoenv.1..1 Rndll DJ nd Tsui TKN.. Ammoni toxicity in fish. Mr Pollut Bull 5, Roumieh R, Brkt A, Adelmeguid NE, Ghnwi J nd Soud, IP. 13. Acute nd chronic effects of queous mmoni on mrled spinefoot ritfish, Signus rivultus (Forsskl 1775). Aqucult Res, org/.1111/j x.

Kor J Fish Aquat Sci 44(6), DOI : 44(6), , 2011 순치기간에따른육봉형산천어 (Oncorhynchus masou masou) 의해수적

Kor J Fish Aquat Sci 44(6), DOI : 44(6), , 2011 순치기간에따른육봉형산천어 (Oncorhynchus masou masou) 의해수적 Kor J Fish Aqut Sci 44(6), 753-758 DOI : http://dx.doi.org/.5657/kfas.211.753, 44(6), 753-758, 211 순치기간에따른육봉형산천어 (Oncorhynchus msou msou) 의해수적응능력 김병기ㆍ김재원 ㆍ박정환 1 ㆍ성기백 2 ㆍ김현주 3 강원도립대학해양생명과학과, 1 노스캐롤라이나주립대학생물학과,