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한별 마
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19 샘플발광억제율 샘플함량단위활성혈액샘플희석율표준품 실측샘플발광억제 샘플희석배율 비교활성 단위활성 혈액혈액

20 억제백분비 대조관측정관대조관

21 활성 대조관측정관대조관 반응액총량채취반응액량 샘플희석배율

23 비효소 샘플군 비효소군 샘플군 비효소군 샘플군단백질 표준농도

30 Tble 1. Effect of ethnol on concentrtions of S-contining mino cids nd the metbolites Time 2 h 4 h 6 h 8 h Methionine SAM SAH Cysteine GSH Hypoturine Turine Turine Group nmole/g liver µmole/g liver µmole/ml serum Control 64 ± ± 4 25 ± ± ± ± ± ± 0.02 Ethnol 58 ± 2 96 ± 4 24 ± 1 97 ± ± ± ± ± 0.03 Control 57 ± ± 5 24 ± ± ± ± ± ± 0.02 Ethnol 83 ± 5* 110 ± 5 27 ± ± ± 0.13*** 0.46 ± 0.04** 5.06 ± 0.25** 0.85 ± 0.13* Control 59 ± ± 4 25 ± ± ± ± ± ± 0.02 Ethnol 79 ± 3* 96 ± 2** 26 ± 2 85 ± 3** 4.04 ± 0.25** 0.46 ± 0.06** 6.92 ± 0.76** 0.92 ± 0.05*** Control 66 ± ± 5 22 ± ± ± ± ± ± 0.04 Ethnol 98 ± 12* 85 ± 7* 45 ± 3*** 100 ± 2* 2.96 ± 0.34*** 0.90 ± 0.16** 5.55 ± 0.41** 1.32 ± 0.09*** 18 h Control 111 ± 2 26 ± ± ± ± ± 0.67 N/D Ethnol 96 ± 4** 33 ± 3** 82 ± 3** 5.41 ± 0.21** 0.11 ± 0.01** 2.04 ± 0.58 N/D 24 h 48 h 120 h Control 62 ± ± 5 25 ± ± ± ± ± ± 0.05 Ethnol 56 ± 7 92 ± 3* 37 ± 1** 93 ± ± 0.22* 0.12 ± 0.02* 2.92 ± ± 0.04 Control 64 ± 5 99 ± 4 23 ± ± ± ± ± ± 0.03 Ethnol 72 ± 6 76 ± 4** 23 ± 2 86 ± 6** 6.94 ± ± 0.02** 1.09 ± 0.13* 0.25 ± 0.01** Control 98 ± 4 23 ± ± ± ± ± ± 0.03 N/D Ethnol 99 ± 2 25 ± ± ± ± ± 0.21* 0.25 ± h Control 107 ± 3 24 ± ± ± ± ± 0.47 N/D Ethnol 105 ± 6 26 ± ± 1* 7.56 ± ± ± 0.34 N/D Ech vlue represents the men SEM for more thn four rts. *,**,*** Significntly different from the control t P < 0.05, 0.01, 0.001, respectively (Student's t-test)

31 Time Group MAT C S C L GCS CDO CDC product formed (nmole)/min/mg protein 2 h 4 h 6 h 8 h 24 h 48 h 120 h 168 h Control ± ± ± ± ± ± 1.1 Ethnol ± ± ±1.5** 6.7 ± ± ± 1.3 Control ± ± ± ± ± ± 1.3 Ethnol ± 50.7 ± 21.8 ± * 2.6* 7.5 ± ± 0.03* 15.2 ± 1.6 Control ± ± ± ± ± ± ± 60.6 ± Ethnol 21.5 ± ± ± 0.10* 0.001** 1.3* 17.1 ± 1.1* Control ± ± ± ± ± ± ± 15.9 ± Ethnol 67.0 ± ± 1.3* 0.57 ± ± * 1.3* Control ± ± ± ± ± ± ± 0.34 ± Ethnol ± ± ± ± ** 0.04** 14.9 ± Control ± ± ± ± ± ± 0.25 ± Ethnol ± ± ± ± *** 0.06** Control ± ± ± ± ± ± 0.4 Ethnol ± ± ± ± ± 0.04** 15.3 ± 1.1 Control ± ± ± ± ± ± 0.9 Ethnol ± ± ± ± ± ±

34 4 4 Weeks Ethnol (mg/ml) Weeks 50 Weeks Time (min) Triglyceride (mg/g liver) *** ** b Mlondildehyde (nmol/g liver) ** ** b 4 Weeks 12 Weeks 50 Weeks 4 Weeks 12 Weeks 50 Weeks

39 1000 TOSC of cytosol control DMN 7dys DMN 14 dys DMN 28 dys 800 TOSC / mg protein b b 0 OH ONOO OOH TOSC / g liver b b b b 0 OH ONOO OOH Figure 2. Totl oxyrdicl scvenging cpcity towrds hydroxyl rdicls, peroxynitrite nd peroxyl rdicls of cytosol Rts were given n intrperitonel injection of DMN (10 µl/kg/dy) 3 times per week (3 consecutive dily injections nd 4 dys off per week) for 1, 2, 4 weeks. Animls were scrificed on dys 7, 14 nd 28 from the initition of exposure. Ech vlue represents the men + SEM for more thn 4 rts. Different letters (, b) indicte siginificnt differences between groups (one-wy ANOVA followed by Newmn-Keuls multiple rnge test, P<0.05)

40 1000 TOSC of microsome control DMN 7dys DMN 14 dys DMN 28 dys TOSC / mg protein b b b c 0 OH ONOO OOH TOSC / g liver b b b c b b b OH ONOO OOH Figure 3. Totl oxyrdicl scvenging cpcity towrds hydroxyl rdicls, peroxynitrite nd peroxyl rdicls of heptic microsome Rts were given n intrperitonel injection of DMN (10 µl/kg/dy) 3 times per week (3 consecutive dily injections nd 4 dys off per week) for 1, 2, 4 weeks. Animls were scrificed on dys 7, 14 nd 28 from the initition of exposure. Ech vlue represents the men + SEM for more thn 4 rts. Different letters (, b, c) indicte siginificnt differences between groups (one-wy ANOVA followed by Newmn-Keuls multiple rnge test, P<0.05)

41 TOSC of serum control DMN 7 dys DMN 14 dys DMN 28 dys TOSC / mg protein OH ONOO OOH TOSC / ml b b b b b b b OH ONOO OOH Figure 5. Totl oxyrdicl scvenging cpcity towrds hydroxyl rdicls, peroxynitrite nd peroxyl rdicls of serum Rts were given n intrperitonel injection of DMN (10 ml/kg/dy) 3 times per week (3 consecutive dily injections nd 4 dys off per week) for 1, 2, 4 weeks. Treted nimls were scrificed on dys 7, 14 nd 28 from the initition of exposure. Ech vlue represents the men + SEM for more thn 4 rts. Different letters (, b) indicte siginificnt differences between groups (one-wy ANOVA followed by Newmn-Keuls multiple rnge test, P<0.05)