212 Research in Plant Disease Vol. 20 No. 3., 7 (Kim, 2004). Colletotrichum (Park Kim, 1992),.. (Agrios, 2008)... 1,202, , 147, 11 (RDA, 2014).

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1 식물병연구 Research Article Open Access Res. Plant Dis. 20(3) : (2014) Protective Activity of the Mixtures of Pine Oil and Copper Hydroxide against Bacterial Spot and Anthracnose on Red Pepper 1 * , 2 *Corresponding author Tel : Fax: tipburn@gmail.com Jae-Woo Soh 1 *,Kyung-Sook Han 1, Seong-Chan Lee 1, Jung-Sup Lee 2 and Jong-Han Park 1 1 Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, Suwon , Korea 2 Apple Research Station, National Institute of Horticultural and Herbal Science, Gunwei , Korea Received July 2, 2014 Revised September 17, 2014 Accepted September 19, 2014 This research was performed to examine the protective activities of the mixtures of pine oil and copper hydroxide against bacterial spot and anthracnose on pepper plants. As for bacterial spot, the treatment of pine oil alone displayed high disease incidence (59.6%) and low protective effect (28.9%). In comparison, the treatments of mixtures and copper hydroxide alone showed protective activities of %. The mixture of pine oil and copper hydroxide (4:1) suppressed the most effectively bacterial spot on pepper. On the other hand, the mixture of pine oil and copper hydroxide (4:1) also showed the strongest protective effect against pepper anthracnose among the 4 treatments tested; its disease incidence and disease control value were 49.8% and 41.7%, respectively. The other treatments showed low protective activities with control values of %. These results suggested that the mixture of pine oil and copper hydroxide (4:1) can be used for the environmental-friendly disease control of bacterial spot and anthracnose on pepper. Keywords : Disease incidence on leaves, Mixture of pine oil and copper hydroxide, Protective activity 29 (RDA-Genebank, 2014),,,,, (Myung, 2006; Yoon, 2014). Xanthomonas campestris pv. vesicatoria,. Research in Plant Disease The Korean Society of Plant Pathology pissn , eissn , (Cook, 1975; Lim and Kim, 1994). Colletotrichum acutatum, Glomerella cingulata, C. coccodes, C. dematium, C. gloeosporioides, C. nigrum, Gloeosporium piperatum (Than, 2008), C. gloesporioides C. acutatum (Park Kim, 1992). Colletotrichum, This is an open access article distributed under the terms of the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

2 212 Research in Plant Disease Vol. 20 No. 3., 7 (Kim, 2004). Colletotrichum (Park Kim, 1992),.. (Agrios, 2008)... 1,202, , 147, 11 (RDA, 2014). (Kim, 2011).,,,,, C. acutatum (Park, 2012). pine oil Botrytis cinerea Pythium aphenidermatum, Fusarium oxysporum, Didymella bryoniae (Steven, 2006) o C %. Colletotrichum acutatum. C. acutatum (potato dextrose agar, PDA) 25 o C ml o C 80%. 공시 약제 및 처리 방법. (pine oil, CAS No , EPA) (Kocide, Dongbu-hitek, Korea)., 100%, 4:1 8: 발병 조사.., 2... 통계 분석. SAS 9.02(statisitical analysis system, SAS Institue, USA) Duncan. 시험 포장 및 시험구 배치. (Supermanitta, Nongwoo bio, Korea) 공시 균주 및 접종 방법. Xanthomonas campestris pv. vesicatoria. X. campestris pv. vesicatoria (nutrient broth agar, NA) 25 o C ml -1 (Fig. 1). PK 4 : 1,,.,. (Table 1)., 83.8%, 59.6%, PK 4:1 20.0%, PK 8:1 27.9%, 23.1%, PK 4 : 1.

26-32 o C 80%. 공시 약제 및 처리 방법. (pine oil, CAS No. 8002-09-3, EPA) (Kocide, Dongbu-hitek, Korea)., 100%, 4:1 8:1. 2 3 2. 발병 조사.., 2... 통계 분석. SAS 9.

3 Research in Plant Disease Vol. 20 No Fig. 1. Control effect of bacterial spot in field by mixing pine oil and copper hydroxide. A: Control, B: Treatment of pine oil only, C: Mixed pine oil and copper hydroxide in the ratio of four to one, D: Mixed pine oil and copper hydroxide in the ratio of eight to one, E: Treatment of copper hydroxide only. Table 1. Controlling effect on bacterial spot by mixing pine oil and copper hydroxide Treatment Ratio of diseased leaves, 0.0%, 28.9%, PK 4:1 76.1%, PK 8:1 66.8%, 72.4%, PK 4:1.. Control efficacy Phytotoxicity Control z 83.8a y 0.0d 0.0a Pine oil only 59.6b 28.9c 0.0a PK 4 : c 76.1a 0.0a PK 8 : d 66.8b 0.0a Copper hydroxide only 23.1c 72.4a 0.0a z The PK is a mixture according to the mixing ratio of each composition of pine oil and copper hydroxide. y The different letters are significantly (P < 0.05) different according to Duncan s multiple test. (Banos, 2003). 4 60% (Park, 2012), Bacillus sp. Pseudomonas sp. (Park, 2006). 4: % 72.4%. 4:1 8:1. 4:1. (Fig. 2)., PK 4:1

copper hydroxide only. Table 1. Controlling effect on bacterial spot by mixing pine oil and copper hydroxide Treatment Ratio of diseased leaves, 0.0%, 28.9%, PK 4:1 76.1%, PK 8:1 66.8%, 72.4%, PK 4:1.

4 214 Research in Plant Disease Vol. 20 No. 3 Fig. 2. Control effect of anthracnose in field by mixing Pine oil and copper hydroxide. A: Control, B: Treatment of pine oil only, C: Mixed pine oil and copper hydroxide in the ratio of four to one, D: Mixed pine oil and copper hydroxide in the ratio of eight to one, E: Treatment of copper hydroxide only. Table 2. Controlling effect on Anthracnose by mixing pine oil and copper hydroxide.. Treatment Ratio of isease Control efficacy Phytotoxicity Control z 85.4a y 0.0d 0.0a Pine oil only 79.1ab 7.4c 0.0a PK 4 : c 41.7a 0.0a PK 8 : b 17.1b 0.0a Copper hydroxide only 71.3b 16.5b 0.0a z The PK is a mixture according to the mixing ratio of each composition of pine oil and copper hydroxide. y The different letters are significantly (P < 0.05) different according to Duncan s multiple test. (Table 2)., 85.4%, 79.1%, PK 4:1 49.8%, PK 8:1 70.7%, 71.3%,, 4:1., 0.0%, 7.4 %, PK 4:1 41.7%, PK 8:1 17.1%, 16.5% PK 4:1,.. 4:1. (Banos, 2003). Colletotrichum acutatum 79.9% 25.6% (Park, 2012). C. gloeosporioides Bacillus sp. (Park, 2012). 41.7%.

copper hydroxide only. Table 2. Controlling effect on Anthracnose by mixing pine oil and copper hydroxide.. Treatment Ratio of isease Control efficacy Phytotoxicity Control z 85.4a y 0.0d 0.

5 Research in Plant Disease Vol. 20 No % 28.9% 4:1 20.0% 76.1%. 79.1% 7.4% 4:1 49.9% 41.7%. 4:1. References Agrios, G.N Plant pathology. Elsevier Acadmic Press. pp Banos, S. B., Lopez, M. H., Molina, E. B. and Wilson, C. L Effects of chitosan and plant extracts on growth of Colletotrichum gloeosporioides, anthracnose levels and quality of papaya fruit. Crop Prot. 22: Cook, A. A Effect of low concentrations of Xanthomonas vesicatoria infiltrated into pepper leaves. Phytopathology 65: Kim, B. D., Park, H. G. and Kim, Y. H Molecular genetics and breeding of chili pepper in Korea: Breeding of varieties to resistant of pepper. Center for Plant Mol. Genet. Breed. Res. pp Kim, G. H., Park, J. Y., Cha, J. H., Jeon, C. S., Hong, S. J., Kim, Y. H., Hur, J. S. and Koh, Y. J Control effect of major fungal diseases of cucumber by mixing of biofungicides registered for control of powdery mildew with other control agents. Korean J. Pestic. Sci. 15: Kwak, Y. K., Kim, I. S., Cho, M. C., Lee, S. C. and Kim, S Growth inhibition effect of environment-friendly farm materials in Colletotrichum acutatum in vitro. J. Bio-Environ. Cont. 21: Lim, Y. S. and Kim, B. S Resistance to bacterial wilt in pepper (Capsicum annuum L.). Korean J. Plant Pathol. 10: Myung, I. S., Hong, S. K., Lee, Y. K., Choi, H. W., Shim, H. S., Park, J. W., Park, K. S., Lee, S. Y., Lee, S. D., Lee, S. H., Choi, H. S., Kim, Y. G., Shin, D. B., Ra, D. S., Yeh, W. H., Han, S. S. and Cho, W. D Review of disease incidences of major crops of the South Korea in Res. Plant Dis. 12: (In Korean) Park, K. S. and Kim, C. H Identification, distribution and etiological characteristics of anthracnose fungi of red pepper in Korea. Korean J. Plant Pathol. 8: Park, S. M., Jung, H. J. and Yu, D. S Screening of an antagonistic bacterium for control of red-pepper anthracnose, Colletotrichum gloeosprioides. J. Life Sci. 16: Park, S. J., Kim, G. H., Kim, A. H., Lee, H. L., Gwon, H. W., Kim, J. H., Lee, K. H. and Kim, H. T Controlling effect of agricultural organic materials on Phytophthora blight and anthracnose in red pepper. Res. Plant Dis. 18:1-9 (In Korean). RDA Information of agricultural materials. go.kr/matenvofood Detail.do. RDA-Genebank Directory finder of Korean plant pathology name. Steven, J.S. Variability associated with suppression of gray mold (Botytis cinerea) on geranium by foliar applications of nonaerated and aerated compost teas. Plant Dis. 90: Than, P. P., Prihastuti, H., Phoulivong, S., Taylor, P. W. J. and Hyde, K. D Chilli anthracnose disease caused by Colletotrichum species. J. Zhejiang Univ. Sci. 9: Yoon, D. H., Oh, S. Y., Nam, K. W., Eom, K. C. and Jung, P. K Changes of cultivation areas and major disease for spicy vegetables by the change of meteorological factors. Climate Change Res. 4:

22: 1087-1092. Cook, A. A. 1975. Effect of low concentrations of Xanthomonas vesicatoria infiltrated into pepper leaves. Phytopathology 65: 487-489. Kim, B. D., Park, H. G. and Kim, Y. H. 2004.

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<30305FC0D3BFF8B8EDB4DC2DB8F1C2F72DC0B1B8AEB1D4C1A4283450292E687770> Korean Journal of Environmental Agriculture Korean J Environ Agric. 2015;34(1):46-51. Korean Online ISSN: 2233-4173 Published online 2015 February 10. http://dx.doi.org/10.5338/kjea.2015.34.1.03 Print