- PDF Free Download

영양세포 영양세포 내생포자

폭기액순환 미생물활성제주입 장치 (2 단 ) 생물반응조 침전조 반송슬러지 잉여슬러지

anaerobic aerobic microaerobic 40mm 0.1mm

폭기액순환 미생물활성제주입 장치 (4 단 ) 생물반응조 침전조 반송슬러지 잉여슬러지

계량조 침전조감속기 장치 폭기조액순환펌프 원수이송펌프 유량조정조 침전조 생물반응조 슬러지반송펌프 송풍기 PROJECT TITLE 차세대핵심기술개발사업 / 하수처리시설 PILOT PLANT DRAWING TITLE FLOW SHEET DRAWN BY H.S.EO CHECKED BY SCALE NONE DESIGNED BY Y.W.LEE APPROVED BY DATE 2001. 11 FILE NAME L-001 SHEET NO

내부순환 계량조 슬러지반송 기기목록표 순번기기번호기기명사양재질수량비고 1 T-01 유량조정조 600Wx1000Lx2400H SS400+TAR EPOXY 1 2 T-02 계량조 250Wx800Lx400H SS400+TAR EPOXY 2 3 M-01 RABC 장치 730Wx1870Lx775Hx0.4 kw - 4 4 T-03 생물반응조 900Wx1000Lx3100H SS400+TAR EPOXY 4 5 T-04 침전조 Φ 2000x2500H GC200 1 6 P-01 A/B 원수펌프 SUBMERSIBLE 40Ax0.05m3 /minx10mhx0.4 kw GC200 2 B BATTERY LIMIT 7 8 9 VORTEX P-02 A/B 폭기조액순환펌프 GC200 32Ax0.2m3 /minx10mhx0.75 kw VORTEX,HORIZONTAL P-03 A/B 슬러지반송펌프 40Ax0.05m3 /minx5mhx0.75 kw GC200 ROTARY B-01 A/B 송풍기 65Ax2.4m3 /minx0.3 kg / m2x3.7 kw GC200 2 2 2 10 D-01 침전조감속기 0.23r.p.mx0.2 kw GC200 1 생물반응조 침전조 유량조정조 BATTERY LIMIT 전기판넬 SEIL-BC 장치 UP 사무실 (CONTAINER) A B' A' 세일기술 ( 주 ) SEIL SEIL TECHNOLOGY CO., LTD PROJECT TITLE 차세대핵심기술개발사업 / 하수처리PILOT 시설 PLANT DRAWING TITLE LAY-OUT DRAWN BY B.S.KIM CHECKED BY DESIGNED BY Y.W.LEE APPROVED BY SCALE 1/60 DATE 11/2001 FILE NAME SHEET NO

폭기액순환 미생물활성제주입 장치 (4 단 ) 생물반응조 침전조 반송슬러지 잉여슬러지

Φ φ

α α

포기액순환 2.0Q 0.5Q RABC 처리수 1.5Q 1.5Q 생물반응조유입유량 3.7Q C 5 생물반응조 생물반응조유출유량 1.7Q C 6 반송슬러지 0.7Q

Δ Δ

100 80 60 40 20 0 100 90 80 70 60 50 40 30 20 10 0 0 10 20 30 40 50 60 70 80 경과일수 (day) 유입 SS 유출 SS 제거율

200 160 120 80 40 0 100 90 80 70 60 50 40 30 20 10 0 0 10 20 30 40 50 60 70 80 경과일수 (day) RABC1 단 RABC4 단유출 BOD 유입 BOD 제거율

250 100 200 80 150 60 100 40 50 20 0 0 0 10 20 30 40 50 60 70 80 90 경과일수 (day) 유입 CODcr RABC1 단 RABC2 단 RABC3 단 RABC4 단 유출CODcr 제거율

80 70 60 50 40 30 20 10 0 균체가RABC 에완전히부착 4 18 32 51 65 79 경과일수 (day) 유입 TN 제거율 유출 TN 100 80 60 40 20 0 40 35 30 25 20 15 10 5 0 1 11 22 32 47 58 68 79 경과일수 (day) 유입 NH4-N 유출 NH4-N 제거율 100 90 80 70 60 50 40 30 20 10 0

3.5 3 2.5 2 1.5 1 0.5 0 1 11 22 32 47 58 68 79 경과일수 (day) 유입 TP 유출 TP 제거율 80 70 60 50 40 30 20 10 0

2.5 2 1.5 1 0.5 0 1 11 22 32 47 58 68 79 경과일수 (day) 유입유출 PO4-P 제거율 100 90 80 70 60 50 40 30 20 10 0

160 140 120 100 80 60 40 20 0 RABC 장치 (20 분 ) 0 5 10 15 20 유입 R A B C 1 R A B C 2 R A B C 3 R A B C 4 생물반응조 (5 시간 ) 침전지 (3 시간 48 분 ) RABC SYSTEM 에서의체류시간 (min) BOD CODCr 548 (min) 유출 ( 방류 )

25 20 15 10 5 0 RABC 장치 (20 분 ) 0 5 10 15 20 유입 R A B C 1 R A B C 2 R A B C 3 R A B C 4 생물반응조 (5 시간 ) 침전지 (3 시간 48 분 ) RABC SYSTEM 에서의체류시간 (min) T-N NH4-N NO2-N NO3-N 548 (min) 유출 ( 방류 ) 3 2.5 2 1.5 1 0.5 0 RABC 장치 (20 분 ) 0 5 10 15 20 유입 R A B C 1 R A B C 2 R A B C 3 R A B C 4 생물반응조 (5 시간 ) 침전지 (3 시간 48 분 ) RABC SYSTEM에서의체류시간 (min) T-P PO4-P 548 (min) 유출 ( 방류 )

포기액 0.1~0.6Q 0.3~1.0Q 유입 Q=250~320 m 3 /d 저류조 RABC 조 (24.75m 3 ) 생물반응조 4 단 ( 각 68.2m 3 ) 침전조 (53.2m 3 ) 방류 0~0.4Q 0.3~0.5Q 잉여슬러지 슬러지반송

포기액 유 입 저류조 RABC조 생물반응조 침전조방류 잉여슬러지 슬러지반송

700 RUN 1 RUN 2 RUN 3 100 600 500 80 농도 (mg/l) 400 300 60 40 제거율 (%) 200 100 20 0 1 6 11 16 21 26 31 36 41 46 51 56 61 66 71 76 81 86 경과일수 ( 일 ) 유입 SS 유출 SS 제거율 0 300 RUN 1 RUN 2 RUN 3 100 250 80 농도 (mg/l) 200 150 100 60 40 제거율 (%) 50 20 0 1 6 11 16 21 26 31 36 41 46 51 56 61 66 71 76 81 86 경과일수 ( 일 ) 유입 BOD 유출 BOD 제거율 0

140 RUN1 RUN2 100 120 80 100 농도 (mg/l) 80 60 60 40 제거율 (%) 40 20 20 0 1 6 11 16 21 26 31 36 41 46 51 56 61 66 71 76 81 경과일수 ( 일 ) 유입 CODMn 유출 CODMn 제거율 0

80 70 60 RUN 1 RUN 2 RUN 3 100 80 농도 (mg/l) 50 40 30 60 40 제거율 (%) 20 10 20 0 1 6 11 16 21 26 31 36 41 46 51 56 61 66 71 76 81 86 경과일수 ( 일 ) 유입 TN 유출 TN 제거율 0 16 14 12 RUN 1 RUN 2 RUN 3 100 80 농도 (mg/l) 10 8 6 60 40 제거율 (%) 4 2 20 0 1 6 11 16 21 26 31 36 41 46 51 56 61 66 71 76 81 86 경과일수 ( 일 ) 유입 TP 유출 TP 제거율 0

SS 14 12 10 8 6 4 2 0 0 5 10 15 20 25 경과일수 (d) 비여과유출수 사여과유출수

BO D 5 50 45 40 35 30 25 20 15 10 5 0 0 5 10 15 20 25 경과일수 (d) 비여과유출수 사여과유출수 CO Dc r 45 40 35 30 25 20 15 10 5 0 0 5 10 15 20 25 경과일수 (d) 비여과유출수 사여과유출수

T -N 30 25 20 15 10 5 0 0 5 10 15 20 25 경과일수 (d) 비여과유출수 사여과유출수 T -P 3.50 3.00 2.50 2.00 1.50 1.00 0.50 0.00 0 5 10 15 20 25 경과일수 (d) 비여과유출수 사여과유출수

350 RUN 1 RUN 2 RUN 3 8.0 RUN 1 RUN 2 RUN 3 300 7.5 250 유입수 ph 7.0 200 150 6.5 반응조전단 반응조후단 100 0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 경과일수 ( 일 ) 6.0 0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 경과일수 ( 일 ) 100 90 RUN 1 RUN 2 RUN 3 250 RUN1 RUN2 RUN3 80 70 200 SV 60 50 40 SVI 150 100 30 20 50 10 반응조전단 반응조후단 0 0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 전단 SVI 후단 SVI 0 0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 경과일수 ( 일 ) 경과일수 ( 일 )

5000 RUN 1 RUN 2 RUN 3 1.6 RUN 1 RUN 2 RUN 3 4500 1.4 MLSS 농도 (mg/l) 4000 3500 3000 2500 2000 1500 1000 500 반응조전단 반응조후단 DO 농도 (mg/l) 1.2 1 0.8 0.6 0.4 0.2 반응조전단 반응조후단 0 0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 경과일수 ( 일 ) 0 0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 경과일수 ( 일 ) 30 RUN 1 RUN 2 RUN 3 1.2 RUN 1 RUN 2 RUN 3 25 1.0 온도 ( ) 20 15 10 0.8 0.6 0.4 0.2 5 0 반응조전단반응조후단 0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 경과일수 ( 일 ) 0.0 0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 경과일수 ( 일 ) 포기액 S-BC 장치 포기액 반응조 슬러지 S-BC 장치 슬러지 반응조

1.0E+06 1.0E+05 1.0E+04 1.0E+03 1.0E+02 1.0E+01 1.0E+00 유입 RABC 2 조 4 조유출사여과 1.0E+11 1.0E+10 1.0E+09 1.0E+08 1.0E+07 1.0E+06 1.0E+05 1.0E+04 1.0E+03 1.0E+02 1.0E+01 1.0E+00 총대장균군 (MPN 법 ) 대장균총대장균군 ( 평판집락법 ) 중온일반세균

1.0E+06 RUN1 RUN2 120.0 1.0E+05 100.0 1.0E+04 80.0 1.0E+03 60.0 1.0E+02 40.0 1.0E+01 20.0 1.0E+00 02/10/15 02/10/17 02/12/12 03/1/8 03/2/14 03/3/26 03/4/24 실험날짜 ( 년 / 월 / 일 ) 0.0 유입수유출수제거율

1.0E+08 RUN2 RUN1 RUN3 RUN2 120.0 1.0E+07 1.0E+06 1.0E+05 100.0 80.0 1.0E+04 60.0 1.0E+03 1.0E+02 1.0E+01 40.0 20.0 1.0E+00 02/10/17 02/12/12 03/1/8 03/2/14 03/3/26 03/4/24 실험날짜 ( ( 년 / / 월 / / 일 )) 0.0 유입수유출수제거율

1.0E+07 RUN1 RUN2 120.0 1.0E+06 100.0 1.0E+05 1.0E+04 1.0E+03 1.0E+02 80.0 60.0 40.0 1.0E+01 20.0 1.0E+00 02/10/17 02/12/12 03/1/8 03/2/14 03/3/26 03/4/24 실험날짜 ( 년 / 월 / 일 ) 유입수유출수제거율 0.0 1.0E+10 RUN1 RUN2 120.0 1.0E+09 1.0E+08 100.0 1.0E+07 1.0E+06 80.0 1.0E+05 1.0E+04 1.0E+03 1.0E+02 1.0E+01 1.0E+00 02/10/15 02/12/12 03/1/8 03/2/14 03/3/26 03/4/24 실험날짜 ( 년 / 월 / 일 ) 60.0 40.0 20.0 0.0 유입수유출수제거율

18.0 RUN1 RUN2 350.0 16.0 14.0 12.0 300.0 250.0 10.0 200.0 8.0 150.0 6.0 4.0 2.0 100.0 50.0 0.0 02/12/12 03/01/18 03/02/14 03/03/26 03/04/24 실험날짜 ( 년 / 월 / 일 ) 0.0 수온 SVI

1.0E+06 1.0E+05 1.0E+04 1.0E+03 1.0E+02 y = 539.88e 0.0283x R 2 = 0.8507 1.0E+01 1.0E+00 0 50 100 150 200 250 300 SS(mg/L)

1.0E+10 1.0E+09 1.0E+08 1.0E+07 1.0E+06 1.0E+05 1.0E+04 y = 818913e 0.0353x R 2 = 0.5467 1.0E+03 1.0E+02 1.0E+01 1.0E+00 0 50 100 150 200 250 300 SS(mg/L)

θ θ μ

θ θ θ θ θ θ

θ θ 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0 0 0.05 0.1 0.15 0.2 0.25 1/ S, L/mg 1.8 1.6 1.4 1.2 1 0.8 0.6 0.4 0.2 0-0.2-0.4-0.6 0 1 2 3 4 5 (S0-S)/Xθ, 1/d

μ θ θ μ

β α α β

Protease 0.20 Biofilm Sludge Specific activity (u/mg) 0.15 0.10 0.05 0.00 5 10 15 20 25 Culture time (hr) Amylase 0.6 0.5 Biofilm Sludge Specific activity (u/mg) 0.4 0.3 0.2 0.1 0.0 5 10 15 20 25 Culture time (hr)

Lipase 0.006 Biofilm Sludge Specific activity (u/mg) 0.004 0.002 0.000 5 10 15 20 25 Culture time (hr)

Biofilm 100 80 Distribution (%) 60 40 20 0 Proteinase activity Amylase activity Lipase activity Total bacteria Hydrolytic bacteira Hydrolytic bacilli Aeration Tank 100 80 Distribution (%) 60 40 20 0 Proteinase activity Amylase activity Lipase activity Total bacteria Hydrolytic bacteira Hydrolytic bacilli

Sludge 100 80 Distribution (%) 60 40 20 0 Proteinase activity Amylase activity Lipase activity Total bacteria Hydrolytic bacteira Hydrolytic bacilli

B. subtilis var. chungkookjang B. subtilis strain B2-00 B. subtilis strain DSM10 B. mojavensis B. subtilis strain ATCC 6633 B. subtilis subsp. spizizenii B. subtilis strain N10 B. subtilis B. vallismortis Ni26 B. atrophaeus B. subtilis subsp. endophyticus B. subtilis strain ATCC 21331 Ni49 B. amyloliquefaciens strain CMB01 B. subtilis strain O9 Ni36 B. subtilis strain C15 B. licheniformis strain KL-164 A A. hydrophila strain 0009 831 A. hydrophila strain M29 A. hydrophila Ni39 A. hydrophila ATCC 35654 A. hydrophila strain T20 A. hydrophila ATCC 7966T A.eucrenophila ATCC 23309T A. encheleia strain A 1881 Haemophilus piscium A. salmonicida A. hydrophila strain 45/90 A. media 16S ribosomal RNA A. media ATCC 33907T A. media 16S rdna B 100 80 Relative activity (%) 60 40 20 0 30 40 50 60 70 80 90 Temperature ( o C)

140 120 Relative activity (%) 100 80 60 40 20 0 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 Ni 26 Ni 36 Ni 39 Ni 49

1.0E+09 1.0E+08 1.0E+07 내생포자 영양세포 1.0E+06 1 2 3 4 5 6 7 9 10 11 12 13 20 21 28 배양시간 ( 일 )

0.5 Carbohydrate (mg/ml) 0.4 0.3 0.2 0.1 0.6 M NaCl 0.0 0 2 4 6 8 10 12 14 16 18 20 22 Fraction No.

일처리할경우를가정하여

구분 환경기술보급 추가기술개발 사업화연도 년 년 년각하수처리장에적극해외중국및동남아 시장제품실용화보급적극진출생명공학분야에접목 제품실용화및시판 항생제및탈취제

< 부록 2-2-1> 경과일수에따른 BOD5 농도변화 (mg/l) 및제거율변화 (%) < 부록 2-2-2> 경과일수에따른 CODMn 농도변화 (mg/l) 및제거율변화 (%)

< 부록 2-2-3> 경과일수에따른 TN 농도변화 (mg/l) 및제거율변화 (%) < 부록 2-2-4> 경과일수에따른 TP 농도변화 (mg/l) 및제거율변화 (%)

< 부록 2-2-5> 유출수의사여과공정에대한실험결과 (mg/l) < 부록 2-2-6> 유입유량변화 (m3/d) < 부록 2-2-7> 생물반응조의 ph 변화

< 부록 2-2-8> 생물반응조의 SV30 변화 < 부록 2-2-9> 생물반응조의 SVI 변화

< 부록 2-2-10> 생물반응조의 MLSS 변화 (mg/l) < 부록 2-2-11> 생물반응조의 DO 변화 (mg/l)

< 부록 2-2-12> 생물반응조의수온변화 ( )

<부록 2-5> 분석된 바실러스균주 Ni1의 16S rdna 염기서열 그림 파일(734bp) - 180 -

Reference : Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. RID: 1058431915-012626-14000 Query= (560 letters) Database: All GenBank+EMBL+DDBJ+PDB sequences (but no EST, STS, GSS, or phase 0, 1 or 2 HTGS sequences) 1,828,583 sequences; 8,622,823,974 total letters If you have any problems or questions with the results of this search please refer to the BLAST FAQs Taxonomy reports Distribution of 109 Blast Hits on the Query Sequence Sequences producing significant alignments: (bits) Value gi 26050884 gb AF539642.1 Bacillus sp. Ni1 16S ribosomal R... 1074 0.0 gi 26050905 gb AF539663.1 Bacillus sp. Ni22 16S ribosomal... 1035 0.0 gi 26050900 gb AF539658.1 Bacillus sp. Ni17 16S ribosomal... 1035 0.0 gi 26050906 gb AF539664.1 Bacillus sp. Ni23 16S ribosomal... 1027 0.0 gi 26050899 gb AF539657.1 Bacillus sp. Ni16 16S ribosomal... 1025 0.0 gi 28274164 gb AY186037.1 Bacillus sp. EBI3 16S ribosomal... 1023 0.0 gi 13560604 gb AF346491.1 AF346491 Bacillus sp. BAC2 16S ri... 1023 0.0 gi 4587041 dbj AB018487.1 Bacillus subtilis gene for 16S r... 1023 0.0 gi 4519613 dbj AB017592.1 Bacillus sp. gene for 16S rrna,... 1023 0.0 gi 4519612 dbj AB017591.1 Bacillus sp. gene for 16S rrna,... 1023 0.0 gi 26050914 gb AF539672.1 Bacillus sp. Ni31 16S ribosomal... 1019 0.0 gi 26050896 gb AF539654.1 Bacillus sp. Ni13 16S ribosomal... 1019 0.0 gi 27652461 gb AY172514.1 Bacillus subtilis isolate BS-1 1... 1017 0.0 gi 27652460 gb AY172513.1 Bacillus subtilis isolate BS-2 1... 1017 0.0 gi 26050917 gb AF539675.1 Bacillus sp. Ni34 16S ribosomal... 1017 0.0 gi 26050901 gb AF539659.1 Bacillus sp. Ni18 16S ribosomal... 1017 0.0 gi 4519611 dbj AB017590.1 Bacillus sp. gene for 16S rrna,... 1017 0.0 gi 4519610 dbj AB017589.1 Bacillus sp. gene for 16S rrna,... 1017 0.0 gi 4519609 dbj AB017588.1 Bacillus sp. gene for 16S rrna,... 1017 0.0 gi 19569754 gb AF489591.1 Bacillus amyloliquefaciens strai... 1015 0.0 gi 19568775 gb AF479349.1 Glacial ice bacterium G200-SD1 1... 1015 0.0 gi 15705859 gb AF411118.1 AF411118 Bacillus sp. Bch1 16S ri... 1015 0.0 gi 9652350 gb AF287011.1 AF287011 Bacillus subtilis strain... 1015 0.0 gi 13928542 dbj AB050667.1 Bacillus sp. PP19-H3 gene for 1... 1015 0.0 gi 7209528 dbj AB021181.1 Bacillus atrophaeus gene for 16S... 1015 0.0 gi 26050910 gb AF539668.1 Bacillus sp. Ni27 16S ribosomal... 1013 0.0 gi 22093909 gb AF399911.1 Bacillus subtilis subsp. endophy... 1013 0.0 gi 28864547 gb AY207468.1 Bacillus subtilis strain KS03 16... 1013 0.0 Alignments >gi 26050884 gb AF539642.1 Bacillus sp. Ni1 16S ribosomal RNA gene, partial sequence Length = 560 Score = 1074 bits (542), Expect = 0.0 Identities = 554/560 (98%) Strand = Plus / Plus Query: 1 cgctgaccgcgtgcctaatnttgcaagtcgagccggacagatgggagcttgctccctgat 60

Sbjct: 1 cgctgaccgcgtgcctaatcttgcaagtcgagccggacagatgggagcttgctccctgat 60 Query: 61 gttagcggcggacgggtgagtaacacgtgggtaacctgcctgtaagactgggataactcc 120 Sbjct: 61 gttagcggcggacgggtgagtaacacgtgggtaacctgcctgtaagactgggataactcc 120 Query: 121 gggaaaccggggctaataccggatggttgtttgaaccgcatggttcanacataaaaggng 180 Sbjct: 121 gggaaaccggggctaataccggatggttgtttgaaccgcatggttcagacataaaaggtg 180 Query: 181 gcttcggctaccacttacagatggacccgcggcgcattagctagttggtgaggtaacggc 240 Sbjct: 181 gcttcggctaccacttacagatggacccgcggcgcattagctagttggtgaggtaacggc 240 Query: 241 tcaccaaggcnacgatgcgtagccgacctgagagggtgatcggccacactgggactgana 300 Sbjct: 241 tcaccaaggcgacgatgcgtagccgacctgagagggtgatcggccacactgggactgaga 300 Query: 301 cacggcccanactcctacgggaggcagcagtagggaatcttccgcaatggacgaaagtct 360 Sbjct: 301 cacggcccagactcctacgggaggcagcagtagggaatcttccgcaatggacgaaagtct 360 Query: 361 gacggagcaacgccgcgtgagtgatgaaggttttcggatcgtaaagctctgttgttaggg 420 Sbjct: 361 gacggagcaacgccgcgtgagtgatgaaggttttcggatcgtaaagctctgttgttaggg 420 Query: 421 aagaacaagtgccgttcaaatagggcggcaccttgacggtacctaaccagaaagccacgg 480 Sbjct: 421 aagaacaagtgccgttcaaatagggcggcaccttgacggtacctaaccagaaagccacgg 480 Query: 481 ctaactacgtgccagcagccgcggtaatacgtaggtggcaagcgttgtccggaattattg 540 Sbjct: 481 ctaactacgtgccagcagccgcggtaatacgtaggtggcaagcgttgtccggaattattg 540 Query: 541 ggcgtaaagggctcgcaggc 560 Sbjct: 541 ggcgtaaagggctcgcaggc 560 >gi 26050905 gb AF539663.1 Bacillus sp. Ni22 16S ribosomal RNA gene, partial sequence Length = 560 Score = 1035 bits (522), Expect = 0.0 Identities = 547/556 (98%), Gaps = 1/556 (0%) Strand = Plus / Plus Query: 1 cgctgaccgcgtgcctaatn-ttgcaagtcgagccggacagatgggagcttgctccctga 59 Sbjct: 5 cgctggccgcgtgcctaattcttgcaagtcgagccggacagatgggagcttgctccctga 64 Query: 60 tgttagcggcggacgggtgagtaacacgtgggtaacctgcctgtaagactgggataactc 119 Sbjct: 65 tgttagcggcggacgggtgagtaacacgtgggtaacctgcctgtaagactgggataactc 124 Query: 120 cgggaaaccggggctaataccggatggttgtttgaaccgcatggttcanacataaaaggn 179 Sbjct: 125 cgggaaaccggggctaataccggatggttgtttgaaccgcatggttcagacataaaaggt 184 Query: 180 ggcttcggctaccacttacagatggacccgcggcgcattagctagttggtgaggtaacgg 239 Sbjct: 185 ggcttcggctaccacttacagatggacccgcggcgcattagctagttggtgaggtaacgg 244 Query: 240 ctcaccaaggcnacgatgcgtagccgacctgagagggtgatcggccacactgggactgan 299 Sbjct: 245 ctcaccaaggcgacgatgcgtagccgacctgagagggtgatcggccacactgggactgag 304 Query: 300 acacggcccanactcctacgggaggcagcagtagggaatcttccgcaatggacgaaagtc 359

Sbjct: 305 acacggcccagactcctacgggaggcagcagtagggaatcttccgcaatggacgaaagtc 364 Query: 360 tgacggagcaacgccgcgtgagtgatgaaggttttcggatcgtaaagctctgttgttagg 419 Sbjct: 365 tgacggagcaacgccgcgtgagtgatgaaggttttcggatcgtaaagctctgttgttagg 424 Query: 420 gaagaacaagtgccgttcaaatagggcggcaccttgacggtacctaaccagaaagccacg 479 Sbjct: 425 gaaaaacaagtgccgttcaaatagggcggcaccttgacggtacctaaccagaaagccacg 484 Query: 480 gctaactacgtgccagcagccgcggtaatacgtaggtggcaagcgttgtccggaattatt 539 Sbjct: 485 gctaactacgtgccagcagccgcggtaatacgtaggtggcaagcgttgtccggaattatt 544 Query: 540 gggcgtaaagggctcg 555 Sbjct: 545 gggcgtaaagggctcg 560 >gi 28274164 gb AY186037.1 Bacillus sp. EBI3 16S ribosomal RNA gene, partial sequence Length = 676 Score = 1023 bits (516), Expect = 0.0 Identities = 533/539 (98%), Gaps = 1/539 (0%) Strand = Plus / Plus Query: 22 tgcaagtcgagccggacagatgggagcttgctccctgatgttagcggcggacgggtgagt 81 Sbjct: 11 tgcaagtcgagc-ggacagatgggagcttgctccctgatgttagcggcggacgggtgagt 69 Query: 82 aacacgtgggtaacctgcctgtaagactgggataactccgggaaaccggggctaataccg 141 Sbjct: 70 aacacgtgggtaacctgcctgtaagactgggataactccgggaaaccggggctaataccg 129 Query: 142 gatggttgtttgaaccgcatggttcanacataaaaggnggcttcggctaccacttacaga 201 Sbjct: 130 gatggttgtttgaaccgcatggttcagacataaaaggtggcttcggctaccacttacaga 189 Query: 202 tggacccgcggcgcattagctagttggtgaggtaacggctcaccaaggcnacgatgcgta 261 Sbjct: 190 tggacccgcggcgcattagctagttggtgaggtaacggctcaccaaggcgacgatgcgta 249 Query: 262 gccgacctgagagggtgatcggccacactgggactganacacggcccanactcctacggg 321 Sbjct: 250 gccgacctgagagggtgatcggccacactgggactgagacacggcccagactcctacggg 309 Query: 322 aggcagcagtagggaatcttccgcaatggacgaaagtctgacggagcaacgccgcgtgag 381 Sbjct: 310 aggcagcagtagggaatcttccgcaatggacgaaagtctgacggagcaacgccgcgtgag 369 Query: 382 tgatgaaggttttcggatcgtaaagctctgttgttagggaagaacaagtgccgttcaaat 441 Sbjct: 370 tgatgaaggttttcggatcgtaaagctctgttgttagggaagaacaagtgccgttcaaat 429 Query: 442 agggcggcaccttgacggtacctaaccagaaagccacggctaactacgtgccagcagccg 501 Sbjct: 430 agggcggcaccttgacggtacctaaccagaaagccacggctaactacgtgccagcagccg 489 Query: 502 cggtaatacgtaggtggcaagcgttgtccggaattattgggcgtaaagggctcgcaggc 560 Sbjct: 490 cggtaatacgtaggtggcaagcgttgtccggaattattgggcgtaaagggctcgcaggc 548 >gi 13560604 gb AF346491.1 AF346491 Bacillus sp. BAC2 16S ribosomal RNA gene, partial sequence Length = 1420 Score = 1023 bits (516), Expect = 0.0 Identities = 533/539 (98%), Gaps = 1/539 (0%) Strand = Plus / Plus

Query: 22 tgcaagtcgagccggacagatgggagcttgctccctgatgttagcggcggacgggtgagt 81 Sbjct: 15 tgcaagtcgagc-ggacagatgggagcttgctccctgatgttagcggcggacgggtgagt 73 Query: 82 aacacgtgggtaacctgcctgtaagactgggataactccgggaaaccggggctaataccg 141 Sbjct: 74 aacacgtgggtaacctgcctgtaagactgggataactccgggaaaccggggctaataccg 133 Query: 142 gatggttgtttgaaccgcatggttcanacataaaaggnggcttcggctaccacttacaga 201 Sbjct: 134 gatggttgtttgaaccgcatggttcagacataaaaggtggcttcggctaccacttacaga 193 Query: 202 tggacccgcggcgcattagctagttggtgaggtaacggctcaccaaggcnacgatgcgta 261 Sbjct: 194 tggacccgcggcgcattagctagttggtgaggtaacggctcaccaaggcgacgatgcgta 253 Query: 262 gccgacctgagagggtgatcggccacactgggactganacacggcccanactcctacggg 321 Sbjct: 254 gccgacctgagagggtgatcggccacactgggactgagacacggcccagactcctacggg 313 Query: 322 aggcagcagtagggaatcttccgcaatggacgaaagtctgacggagcaacgccgcgtgag 381 Sbjct: 314 aggcagcagtagggaatcttccgcaatggacgaaagtctgacggagcaacgccgcgtgag 373 Query: 382 tgatgaaggttttcggatcgtaaagctctgttgttagggaagaacaagtgccgttcaaat 441 Sbjct: 374 tgatgaaggttttcggatcgtaaagctctgttgttagggaagaacaagtgccgttcaaat 433 Query: 442 agggcggcaccttgacggtacctaaccagaaagccacggctaactacgtgccagcagccg 501 Sbjct: 434 agggcggcaccttgacggtacctaaccagaaagccacggctaactacgtgccagcagccg 493 Query: 502 cggtaatacgtaggtggcaagcgttgtccggaattattgggcgtaaagggctcgcaggc 560 Sbjct: 494 cggtaatacgtaggtggcaagcgttgtccggaattattgggcgtaaagggctcgcaggc 552 >gi 4587041 dbj AB018487.1 Bacillus subtilis gene for 16S rrna, partial sequence, strain:atcc 21331 Length = 1504 Score = 1023 bits (516), Expect = 0.0 Identities = 533/539 (98%), Gaps = 1/539 (0%) Strand = Plus / Plus Query: 22 tgcaagtcgagccggacagatgggagcttgctccctgatgttagcggcggacgggtgagt 81 Sbjct: 31 tgcaagtcgagc-ggacagatgggagcttgctccctgatgttagcggcggacgggtgagt 89 Query: 82 aacacgtgggtaacctgcctgtaagactgggataactccgggaaaccggggctaataccg 141 Sbjct: 90 aacacgtgggtaacctgcctgtaagactgggataactccgggaaaccggggctaataccg 149 Query: 142 gatggttgtttgaaccgcatggttcanacataaaaggnggcttcggctaccacttacaga 201 Sbjct: 150 gatggttgtttgaaccgcatggttcagacataaaaggtggcttcggctaccacttacaga 209 Query: 202 tggacccgcggcgcattagctagttggtgaggtaacggctcaccaaggcnacgatgcgta 261 Sbjct: 210 tggacccgcggcgcattagctagttggtgaggtaacggctcaccaaggcaacgatgcgta 269 Query: 262 gccgacctgagagggtgatcggccacactgggactganacacggcccanactcctacggg 321 Sbjct: 270 gccgacctgagagggtgatcggccacactgggactgagacacggcccagactcctacggg 329 Query: 322 aggcagcagtagggaatcttccgcaatggacgaaagtctgacggagcaacgccgcgtgag 381 Sbjct: 330 aggcagcagtagggaatcttccgcaatggacgaaagtctgacggagcaacgccgcgtgag 389

Query: 382 tgatgaaggttttcggatcgtaaagctctgttgttagggaagaacaagtgccgttcaaat 441 Sbjct: 390 tgatgaaggttttcggatcgtaaagctctgttgttagggaagaacaagtgccgttcaaat 449 Query: 442 agggcggcaccttgacggtacctaaccagaaagccacggctaactacgtgccagcagccg 501 Sbjct: 450 agggcggcaccttgacggtacctaaccagaaagccacggctaactacgtgccagcagccg 509 Query: 502 cggtaatacgtaggtggcaagcgttgtccggaattattgggcgtaaagggctcgcaggc 560 Sbjct: 510 cggtaatacgtaggtggcaagcgttgtccggaattattgggcgtaaagggctcgcaggc 568 Database: All GenBank+EMBL+DDBJ+PDB sequences (but no EST, STS, GSS, or phase 0, 1 or 2 HTGS sequences) Posted date: Jul 16, 2003 11:14 PM Number of letters in database: -24,322,264 Number of sequences in database: 1,815,295 Lambda K H 1.37 0.711 1.31 Gapped Lambda K H 1.37 0.711 1.31 Matrix: blastn matrix:1-3 Gap Penalties: Existence: 5, Extension: 2 Number of Hits to DB: 1,302,016 Number of Sequences: 1828583 Number of extensions: 1302016 Number of successful extensions: 236832 Number of sequences better than 10.0: 85739 Number of HSP's better than 10.0 without gapping: 85532 Number of HSP's successfully gapped in prelim test: 207 Number of HSP's that attempted gapping in prelim test: 21163 Number of HSP's gapped (non-prelim): 196391 length of query: 560 length of database: 8,622,823,974 effective HSP length: 21 effective length of query: 539 effective length of database: 8,584,423,731 effective search space: 4627004391009 effective search space used: 4627004391009 T: 0 A: 0 X1: 6 (11.9 bits) X2: 15 (29.7 bits) S1: 12 (24.3 bits) S2: 20 (40.1 bits)