식물병연구 Research Article Open Access Res. Plant Dis. 20(3) : 211-215(2014) http://dx.doi.org/10.5423/rpd.2014.20.3.211 Protective Activity of the Mixtures of Pine Oil and Copper Hydroxide against Bacterial Spot and Anthracnose on Red Pepper 1 * 1 1 2 1 1, 2 *Corresponding author Tel : +82-31-290-6243 Fax: +82-31-290-6259 E-mail: tipburn@gmail.com Jae-Woo Soh 1 *,Kyung-Sook Han 1, Seong-Chan Lee 1, Jung-Sup Lee 2 and Jong-Han Park 1 1 Department of Horticultural Environment, National Institute of Horticultural and Herbal Science, Suwon 440-706, Korea 2 Apple Research Station, National Institute of Horticultural and Herbal Science, Gunwei 716-812, Korea Received July 2, 2014 Revised September 17, 2014 Accepted September 19, 2014 This research was performed to examine the protective activities of the mixtures of pine oil and copper hydroxide against bacterial spot and anthracnose on pepper plants. As for bacterial spot, the treatment of pine oil alone displayed high disease incidence (59.6%) and low protective effect (28.9%). In comparison, the treatments of mixtures and copper hydroxide alone showed protective activities of 66.8-76.1%. The mixture of pine oil and copper hydroxide (4:1) suppressed the most effectively bacterial spot on pepper. On the other hand, the mixture of pine oil and copper hydroxide (4:1) also showed the strongest protective effect against pepper anthracnose among the 4 treatments tested; its disease incidence and disease control value were 49.8% and 41.7%, respectively. The other treatments showed low protective activities with control values of 7.4-17.1%. These results suggested that the mixture of pine oil and copper hydroxide (4:1) can be used for the environmental-friendly disease control of bacterial spot and anthracnose on pepper. Keywords : Disease incidence on leaves, Mixture of pine oil and copper hydroxide, Protective activity 29 (RDA-Genebank, 2014),,,,, (Myung, 2006; Yoon, 2014). Xanthomonas campestris pv. vesicatoria,. Research in Plant Disease The Korean Society of Plant Pathology pissn 1598-2262, eissn 2233-9191.., (Cook, 1975; Lim and Kim, 1994). Colletotrichum acutatum, Glomerella cingulata, C. coccodes, C. dematium, C. gloeosporioides, C. nigrum, Gloeosporium piperatum (Than, 2008), C. gloesporioides C. acutatum (Park Kim, 1992). Colletotrichum, This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
212 Research in Plant Disease Vol. 20 No. 3., 7 (Kim, 2004). Colletotrichum (Park Kim, 1992),.. (Agrios, 2008)... 1,202, 256 92, 147, 11 (RDA, 2014). (Kim, 2011).,,,,, C. acutatum (Park, 2012). pine oil Botrytis cinerea Pythium aphenidermatum, Fusarium oxysporum, Didymella bryoniae (Steven, 2006).... 24-28 o C 80-100%. Colletotrichum acutatum. C. acutatum (potato dextrose agar, PDA) 25 o C 2 10 6 ml -1. 26-32 o C 80%. 공시 약제 및 처리 방법. (pine oil, CAS No. 8002-09-3, EPA) (Kocide, Dongbu-hitek, Korea)., 100%, 4:1 8:1. 2 3 2. 발병 조사.., 2... 통계 분석. SAS 9.02(statisitical analysis system, SAS Institue, USA) Duncan. 시험 포장 및 시험구 배치. (Supermanitta, Nongwoo bio, Korea) 2 10 50 5 2 750. 50 3. 공시 균주 및 접종 방법. Xanthomonas campestris pv. vesicatoria. X. campestris pv. vesicatoria (nutrient broth agar, NA) 25 o C 2 10 6 ml -1 (Fig. 1). PK 4 : 1,,.,. (Table 1)., 83.8%, 59.6%, PK 4:1 20.0%, PK 8:1 27.9%, 23.1%, PK 4 : 1.
Research in Plant Disease Vol. 20 No. 3 213 Fig. 1. Control effect of bacterial spot in field by mixing pine oil and copper hydroxide. A: Control, B: Treatment of pine oil only, C: Mixed pine oil and copper hydroxide in the ratio of four to one, D: Mixed pine oil and copper hydroxide in the ratio of eight to one, E: Treatment of copper hydroxide only. Table 1. Controlling effect on bacterial spot by mixing pine oil and copper hydroxide Treatment Ratio of diseased leaves, 0.0%, 28.9%, PK 4:1 76.1%, PK 8:1 66.8%, 72.4%, PK 4:1.. Control efficacy Phytotoxicity Control z 83.8a y 0.0d 0.0a Pine oil only 59.6b 28.9c 0.0a PK 4 : 1 20.0c 76.1a 0.0a PK 8 : 1 27.9d 66.8b 0.0a Copper hydroxide only 23.1c 72.4a 0.0a z The PK is a mixture according to the mixing ratio of each composition of pine oil and copper hydroxide. y The different letters are significantly (P < 0.05) different according to Duncan s multiple test. (Banos, 2003). 4 60% (Park, 2012), Bacillus sp. Pseudomonas sp. (Park, 2006). 4:1. 76.1% 72.4%. 4:1 8:1. 4:1. (Fig. 2)., PK 4:1
214 Research in Plant Disease Vol. 20 No. 3 Fig. 2. Control effect of anthracnose in field by mixing Pine oil and copper hydroxide. A: Control, B: Treatment of pine oil only, C: Mixed pine oil and copper hydroxide in the ratio of four to one, D: Mixed pine oil and copper hydroxide in the ratio of eight to one, E: Treatment of copper hydroxide only. Table 2. Controlling effect on Anthracnose by mixing pine oil and copper hydroxide.. Treatment Ratio of isease Control efficacy Phytotoxicity Control z 85.4a y 0.0d 0.0a Pine oil only 79.1ab 7.4c 0.0a PK 4 : 1 49.8c 41.7a 0.0a PK 8 : 1 70.7b 17.1b 0.0a Copper hydroxide only 71.3b 16.5b 0.0a z The PK is a mixture according to the mixing ratio of each composition of pine oil and copper hydroxide. y The different letters are significantly (P < 0.05) different according to Duncan s multiple test. (Table 2)., 85.4%, 79.1%, PK 4:1 49.8%, PK 8:1 70.7%, 71.3%,, 4:1., 0.0%, 7.4 %, PK 4:1 41.7%, PK 8:1 17.1%, 16.5% PK 4:1,.. 4:1. (Banos, 2003). Colletotrichum acutatum 79.9% 25.6% (Park, 2012). C. gloeosporioides Bacillus sp. (Park, 2012). 41.7%.
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