KISEP Rhinology Korean J Otolaryngol 2000;43:40-4 비용에서 스테로이드 투여 후 활성산소의 생성과 항산화효소 활성도의 변화 조진학 김현웅 김희동 오원희 정인교 정 근 임현준 The Changes of the Generation of Superoxide Free Radical and the Activitiy of Antioxidant Enzymes after Streroid Therapy in Nasal Polyps Jin Hak Cho, MD, Hyun Ung Kim, MD, Hee Dong Kim, MD, Won Hee Oh, MD, Yin Gyo Jung, MD, Keun Chung, MD and Hyun Joon Lim, MD Department of Otorhinolaryngology-Head & Neck Surgery, College of Medicine, Hallym University, Chunchon, Korea ABSTRACT Background and ObjectivesAn intranasal topical and a systemic steroid therapy produce clinical improvement in patients with nasal polyps, but the mechanisms of their action are not clear. Recently, reactive oxygen species such as oxygen free radicals were identified as the potent toxic agents in various pathologic conditions including nasal polyps. The purposes of this study were to reveal the steroid effect on the generation of oxygen free radicals and exmaine the activities of various antioxidant enzymes in human nasal polyps. Materials and MethodsForty patients who received endoscopic sinus surgery due to chronic paranasal sinusitis with nasal polyps were classified into four groupsgroup I included 10 specimens with no medication, group II included 10 specimens with oral steroid medication, Group III included 10 specimens with intranasal topical steroid therapy, group IV included 10 specimens with both oral and intranasal topical steroid therapy. We measured the generation of oxygen free radicals and activity of antioxidant enzymes SOD, GSH-Px, and CAT, and compared each group using electrophotometric analysis. ResultsThe generation of oxygen free radicals were lowered in group II, III, and IV, compared to group I. Group IV showed the lowest level in the generation of oxygen free radicals. The activities of SOD, GSH-Px, and CAT, which are specific scavengers of oxygen free radicals, increased significantly in groups II, III, and IV p0.05. ConclusionThese results suggest that oxygen free radicals may play an important roles in the formation of nasal polyps. The mechanism of steroid action in the treatment of polyps may be explained in the decreased generation of oxygen free radicals and the increased activity of antioxidant enzymes in some ways. Korean J Otolaryngol 2000;43:40-4 KEY WORDSNasal polyp Oxygen free radical Antioxidant enzyme. 40
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Fig. 1. The amount of oxygen free radical production in group I, II, III and IV. Vertical bars are meansd. *p0.05, *significantly different from group I p0.05. Fig. 3. Catalase activity in group I, II, III and IV. Vertical bars are meansd. *p0.05 Fig. 2. Superoxide dismutase activity in group I, II, III and IV. Vertical bars are meansd. *p0.05 42 Fig. 4. Glutathione peroxidase activity in group I, II, III and IV. Vertical bars are meansd. *p0.05 Korean J Otolaryngol 2000;43:40-4
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REFERENCES 1) Kim DJ, Kim IT. Measurement of superoxide free radicals and xanthine oxidase in nasal polyps. Korean J Otolaryngol 199538 75-80. 2) Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 197632248-54. 3) Auchlar C, Voisin E. Handbook of methods for oxygen radical research. Boca Raton. Florida CRC Press1984. p.123-32. 4) Hyland K, Voisin E, Banoun H, Auclair C. Superoxide dismutase assay using alkaline dimethylsulfoxide as superoxide anion-generating system. Anal Biochem 1983135280-7. 5) Nelson DP, Kiesow LA. Enthalpy of decomposition of hydrogen peroxide by catalase at 25 with molar extinction coefficients of H 2 O 2 solutions in the UV. Anal Biochem 197249474-8. 6) Paglia ED, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathion peroxidase. J Lab Clin Med 196770158-69. 7) Blumstein GI. Nasal polyps. Arch Otolaryngol 196683266-9. 8) Liu CM, Shun CT, Hsu MM. Lymphocyte subsets and antigenspecific IgE antibody in nasal polyps. Ann Allergy 19947219-24. 9) Larsen PL, Tos M, Kuijpers W, van der Beek JM. The early stages of polyp formation. Laryngoscope 1992102670-7. 10) Atalla SL, Toledo-Pereyra LH, Mackenzie GH, Cerderna JP. Influence of oxygen-derived free radical scavengers on ischemic livers. Transplantation 198540584-90. 11) Guice KS, Miller DE, Oldham KT, Townsend CM Jr, Thompson JC. Superoxide dismutase and catalase A possible role in established pancreatitis. Amer J Surgery 1986151163-9. 12) Sagi A, Ferder M, Levens D, Strauch B. Improved survial of island flaps after prolonged ischemia by perfusion with superoxide dismutase. Plast Reconstr Surg 198677639-44. 13) Seidman MD, Quirk WS, Nuttall AL, Schweitzer VG. The protective effects of allopurinol and superoxide dismutase polyethylene glycol on ischemia and reperfusion induced cochlear damage. Otolaryngol Head Neck Surg 1991105457-63. 14) Seidman MD, Shivapuja BG, Quirk WS. The protective effects of allopurinol and superoxide dismutase on noise induced cochlear damage. Otolaryngol Head Neck Surg 19931091052-6. 15) Harman D. The aging process. Proc Natl Acad Sci USA 198178 7124-8. 16) Ames BN. Dietary carcinogens and anticarcinogens; Oxygen radical and degenerative disease. Science 1983231256-64. 17) Yoshikawa T, Naito Y, Ueda S, Oyamada H, Takemura T, Yoshida N, et al. Role of oxygen-derived free radicals in the pathogenesis of gastric mucosal lesions in rats. J Clin Gastroenterol Suppl 1990165-71. 18) Manson PN, Anthenelli RM, Im MJ, Bukley GB, Hoopes JE. The role of oxygen free radicals in ischemic tissue injury in island skin flaps. Ann Surg 198319887-90. 19) Kim DJ. Levels of superoxide free radicals and antioxidant enzymes in nasal polyps. Korean J Otolaryngol 1995381917-21. 20) Richard LM. Pharmacotherapy of allergic rhinitis Corticosteroids. Otolaryngol Head Neck Surg 1995113120-5. 44 Korean J Otolaryngol 2000;43:40-4