untitled
|
|
- 서진 두
- 5 years ago
- Views:
Transcription
1 대한화상학회지제 12 권제 1 호 38 Journal of Korean Burn Society Vol. 12, No. 1, 38-42, 2009 CD34 인체제대혈유래단핵구세포가피부창상치유촉진에미치는영향 박상은ㆍ유대현ㆍ탁관철ㆍ나동균 연세대학교의과대학성형외과학교실, 인체조직복원연구소 Acceleration of Wound Regeneration by Implantation of CD34 Mononuclear Cell from Human Cord Blood Sang Eun Park, M.D., Dae Hyun Lew, M.D., Kwan Chul Tark, M.D. and Dong Kyun Rah, M.D. Department of Plastic and Reconstructive Surgery, Yonsei University College of Medicine, Institute for Human Tissue Restoration, Seoul, Korea 책임저자 : 유대현, 서울시서대문구신촌동 , 연세대학교의과대학성형외과학교실 Tel: , Fax: dhlew@yuhs.ac Purpose: Cord blood stem cells are easily obtainable and have characteristics of higher proliferative potential and lower immunogenicity and with benefits in allogenic transplantation for wound treatment. To provide treatment evidence of cord blood stem cell on burn injury, CD34+ (hematopoietic lineage) and CD34 cells (mesenchymal stem cells lineage) were isolated and applied to full thickness skin wounds for evaluation of their effect. Methods: Mononuclear cell layer was separated by Ficoll- Paque from human cord blood, followed by selection of CD34 +, cells. Two full-thickness wounds were made on dorsal area of ICR mice with 8 mm punch. CD 34+, cell suspension was injected into the wound sites and wound healing rate was measured every 3 days with analysis of neovascularization. Immunohistochemical study for stem cell survival and TGF-β1 was performed. Results: The wound healing rate of CD34, CD34+ and control group was 34.73%, 9.24%, 5.132% on day 3 and 82.60%, 64.45% 76.20% on day 9 respectively. The CD34 group showed a significantly higher wound healing rate (p <0.01). The CD34 group revealed a relatively well organized epidermis and showed a remarkably increased neo-vascularization. Anti-human nuclei was detected on CD 34 group. TGF-β1 was stained all groups with no differences. Conclusion: CD34 mononuclear cells group showed increased wound healing than the control and CD34+ mononuclear cells treated group. CD34 mononuclear cells enhanced epithelization and neo-vascularization by means of differentiation into endothelial cells, myofibroblast and so on. We can conclude that isolated CD34 mononuclear cells can be helpful for burn wound. (Journal of Korean Burn Society 2009;12:38-42) Key Words: Cord blood stem cell transplantation, Wound healing, Leukocytes, Mononuclear 서 오늘날줄기세포의창상치유에대한많은연구들이진행중이다. 이중에서인체의제대혈에서유래한줄기세포는골수에서얻은줄기세포에비해미성숙하고높은증식력을가진다 1-4). 게다가제공자에게서위험없이풍부한양을얻을수있고 5), 면역반응이경미하므로이식에용이하다 6). 이러한제대혈유래줄기세포는재생의학의치료방법으로서많은장점을가지고있다. 인체제대혈은조혈모세포뿐만아니라조직재생의과정에서중요한역할을하는비조혈모세포로도분화할수있다는연구들이발표되고있다 7-9). 많은연구자들은줄기세포가창상치유를향상시킨다고보고하고있는데, Badiavas 등 10) 은상처를입은후 21일간의조직치유과정중에골수세포들이 perifollicular cells, blood vessel cells, perisebaceous gland cells로바뀔수있다고보고하였고몇몇의임상적연구에서는조직허혈, 혈관염등으로인한만성피부궤양을치료하는데골수의세포를투여함으로써좋은효과를얻을수있다는것을보여주었다 11,12). Valbonesi 등 13) 은기초적인수준의연구에서제대혈모세포가염증과숙주대조직반응없이피부조직의상처치유를향상시킨다는보고를하였고, Yamaguchi 등 14) 은투여한골수세포가상처조직에서근섬유아세포 (myofibroblasts) 로분화를일으켜조직의치유를증가시킨다고주장하였다. 우리는이번연구에서인체제대혈유래단핵구세포, 특히조혈모세포계열로알려진 CD34+ 단핵구세포가신생혈관생성을증가시킴으로써, 피부창상치유를향상시킬 론
2 박상은등 :CD34 인체제대혈유래단핵구세포가피부창상치유촉진에미치는영향 39 수있을것이라가정하여인체유래제대혈단핵구세포가창상의크기를줄이는정도와신생혈관생성정도를객관적으로살펴보았다. 특히창상에국소적으로투여된인체제대혈유래단핵구세포가창상의주변조직에서살아남아창상치유와신생혈관생성을촉진시킨다고알려진 TGF-β1 의분비량을증가시킬것이라가정하였다. 재료및방법 1. 제대혈로부터 CD34+/ CD34 세포의분리인체제대혈의수득은어머니의동의를받고연세의료원분만실에서출산직후이루어졌다. 태반이제거된후, 탯줄로부터무균, 24.5 ml의헤파린처리가된 PVC백으로제대혈을 250 cc 정도모은후 24시간내에모든실험과정을시행하였다. 제대혈을저밀도분획 (Ficoll-Paque plus; g/l; Amersham Biosciences, UK) 을이용하여단핵구세포를분리하고세척하였다. 이후 CD34 전구세포분리킷 (CD 34 progenitor cell isolation kit, Miltenyi Biotech, Germany) 을사용하여단핵구세포를 CD 34+, 세포로분리하였다. 실험군으로약 제대혈유래 CD 34+ & 단핵구세포를각각 0.2 ml PBS (phosphate buffered saline) 용액에넣고, 대조군으로는 0.2 ml PBS 용액만을준비하였다. 2. 동물실험모델에서의창상형성모든동물실험은 Guide for the Care and Use of Laboratory Animals (National Institutes of Health [NIH] publication No ) 에따라시행되었으며연세대학교실험동물위원회의승인을받았다. 동물실험모델로는중앙실험동물 (Central Lab animal, Inc., Korea) 에서공급받고 AAALAC (Association for Assessment and Accreditation of Laboratory Animal Care) 인증된연세대학교산하실험동물부사육실에서키워진 ICR 쥐를사용하였다. 창상형성을위해복강마취 (ketamine 30 mg/kg, xylazine 3 mg/kg) 하에생후 4주된 10마리의 ICR 쥐의등쪽두부에서미부까지의털을제거한후, 직경 8 mm의피부조직검사용펀치 (Biopsy punch, Kai medical, Japan) 를이용, panniculus carnosus 근육층을포함한 2개의원형전층피부결손을만들었다. 생성된피부결손은 Tegaderm R (3M Health Care, St Paul, MN, USA) 를이용하여창상이마르지않고조직액이고이도록 24시간유지시켰다. 이후실험군으로 CD 34+와 34 세포액을국소적으로각각 5마리의 ICR 쥐의두피부결손중한쪽에투여하고, 대조군으로 0.2 ml PBS 용액만을 10마리의남은한쪽의피부결손에 투여하였다. 3. 창상의평가 1) 상피화정도를통한창상치유속도측정줄기세포투여후 3일간격으로 12일까지창상의상피화및수축정도를사진을통해측정하였다. 개방창의크기는 computer planimetry (Scion-image R ; NIH-Scion corporation, USA) 을이용하여면적을산출하였다. 2) 조직검사를통한혈행화정도측정실험 3, 6, 9, 12일째창상부위조직 (10 mm 인접부위 ) 을채취하여 H&E 염색을실시하고무작위로 10군데를선택하여고배율시야 (HPF 400, Olympus DP12- PC, Tokyo, Japan) 당존재하는신생혈관의개수를특정하였다. 3) 면역조직화학염색을통한창상내투여세포발현유무와 TGF-β1 발현정도실험 12일째창상조직을채취하여창상조직에서제대혈유래줄기세포의생존여부를확인하기위해 Anti human nuclei Ab (MAB1281, Chemicon International Inc., Massachusetts, USA) 를일차항원으로사용하였고,α-SMA (smooth muscle actin) 과수축력, 신생혈관생성을증가시킨다고알려진 TGF-β의분비를증가시키는지여부를확인하기 Anti mouse & human TGF-β1 Ab (Santa cruz, sc- 146, CA, USA) 를일차항원으로사용하였다. 4) 통계분석모든자료의통계처리는 Excel 2003 (The office, Microsoft, USA) 를이용하여수행하였으며, 창상치유율의분석을위해 Student s T-test를이용하여유효성여부를검증하였다. 모든값은평균 ± 표준편차 (mean±sd) 로표시하였으며, p값이 0.01 미만인경우를통계적으로유의한것으로평가하였다. 결과 1. 창상의크기변화 10마리의쥐를대상으로한실험에서실험시작후 12일까지제대혈유래 CD34 단핵구세포를투여한군에서 CD34+ 단핵구세포를투여한군이나대조군과비교하여통계적으로유의하게높은창상치유율을보였다 (Fig. 1, p-value=0.005<0.01). 특히, 실험후 3일째까지 CD34 단핵구투여군은 CD34+나대조군에비해높은창상치유증가속도를보였다. CD34+ 단핵구투여군과대조군간의유의한차이는없었다 (p-value=0.172>0.01).
3 40 대한화상학회지 Vol. 12, No. 1, 2009 였다. CD34 단핵구 투여군은 고배율 시야(HPF 400)당 2. 조직학적 변화 평균 8.48개의 신생 혈관 개수를 보였으나 CD34 단핵구 실험 3, 6, 9, 12일째의 조직으로부터 상피층의 형태학적 투여군과 대조군은 각각 2.78, 3.64개의 신생 혈관 개수만을 변화와 신생혈관 생성 정도를 조사하였다. 6일째 H/E 염색 보였다. 에서 CD34 단핵구 투여군은 CD34 단핵구 투여군이나 대조군에 비해 상대적으로 빠르고 잘 조직화된 상피층을 3. 면역조직화학염색 보였다(Fig. 2). 실험 6일째, 창상 부위의 조직에서 생존하고 있는 인체 실험 12일째 조직의 진피층에서 신생 혈관의 개수를 측 제대혈 유래 단핵구 세포를 확인하기 위해 Anti-human 정하였다(Fig. 3). 신생 혈관 개수는 H/E 조직염색의 고배 nuclei Ab에 대한 면역조직화학염색을 시행하였다(Fig. 4). 율 시야(HPF 400)상에서 무작위로 선정된 신생 진피층 부 CD34 단핵구 투여군에서는 염색 후 갈색의 DAB (dia- 위의 사진 10장에서 얻어진 혈관 개수의 평균으로 측정하 minobenzidine) 양성 반응을 보였으나 대조군에서는 양성 반응을 보이지 않았다(Fig. 4). Fig. 1. Wound healing rate (%) after transplantation of human CB-derived CD34 / mononuclear cells in mice. The CD34 CB-derived mononuclear cells group has higher wound healing rate than CD34 and control group. Especially, until 3 days CD34 mononuclear cell group revealed high acceleration. Fig days after transplantation, the numbers of newly formed vessels per high-power field ( 400). Neovascularization was examined as mean value counted in 10 sectional areas acquired from wound lesion. Fig. 2. Epithelialization on day 6 after transplantation. CD34 mononuclear cell group (left) had relatively well organized epidermis faster than control group (right).
4 박상은등 :CD34 인체제대혈유래단핵구세포가피부창상치유촉진에미치는영향 41 Fig. 4. On day 6, the specimen of wound lesion analysed with immunohistochemistry. Anti-human nuclei Ab as a specific marker for human CB mononuclear cells was applied. Immunohistochemstry of CD34 mononuclear cells treated group (left, 40) showed brown colored DAB+ in the wound lesion. But, control group did t (right, 40). 인체제대혈유래단핵구세포를투여가 TGF-β1 분비량차이에미치는영향을알아보기위해 Anti mouse & human TGF-β1 Ab을이용한면역조직화학염색을시행하였다. 면역조직화학염색상, CD34 단핵구투여군과대조군모두갈색으로염색된 DAB 양성반응을보이며유의한차이를보이지않았다. 고찰이번연구에서저자들은인체제대혈유래단핵구세포중 CD34+ 단핵구세포가신생혈관생성을증가시킴으로써피부창상치유를향상시킬것이라는가정하에인체제대혈에서 CD34+ 단핵구와 CD34 단핵구세포를분리하였다. CD34+ 단핵구세포는적혈구등으로분화하는것으로알려진조혈모세포계열이다. CD34+ 단핵구세포가신생혈관생성을증가시키는혈관내피전구세포로분화를함으로써, CD34+ 단핵구투여군이 CD34 단핵구투여군이나대조군보다향상된창상치유율을보일것이라기대하였으나실제로는 CD34 단핵구투여군이 CD34+ 단핵구투여군이나대조군보다현저하게향상된창상치유율을보였다. CD34 단핵구투여군은다른군에비해더빨리상피화 (epithelialization) 가진행되었고신생혈관생성이증가되었으며, 또한투여한세포가창상부위에서생존하고있었다. Volk 등 15) 은골수유래단핵구세포가근섬유아세포활성을증가시키는 CTGF (connective tissue growth factor), TGF-β1 등의여러성장인자를분비하고혈관내피세포동원을향상시키며혈관의재형성을조절하는 VEGF나 CTGF (connective tissue growth factor) 등의여러 chemokine을생성할수있다고발표하였다. 게다가골수유래단핵구세포는각질세포 (keratinocyte) 의증식과이동에중요한여러성장인자 (Heparin-binding EGF-like growth factor, TGFα, EGF) 를생산할수있고골수유래단핵구세포에서분리한중간엽줄기세포는비슷한방식을통해골수유래단핵구세포보다더강한창상치유능력을보인다는것을밝혀냈다. 이러한연구결과를통해인체제대혈유래 CD34 단핵구세포는상피화와신생혈관생성을향상시킴으로써피부창상치유를촉진하고있는데이는인체제대혈유래 CD34 단핵구세포계열에속해있는중간엽줄기세포가이러한작용을하기때문이라유추할수있다. 이것은 2가지방법으로설명될수있는데, 첫번째로 CD34 줄기세포가창상치유를향상시키는상피세포, 내피세포전구세포, 근섬유아세포로직접분화하여작용한결과라생각할수있고, 두번째로 CD34 줄기세포에서분비되는 TGF-β1 등의성장인자나 cytokines 같은체액인자 (humoral factor) 가창상치유를촉진하는것으로생각할수있다. 전자의경우, 창상에투여된인체제대혈유래줄기세포가재상피화 (integrin α3), 혈관내피전구세포 (endoglin-cd105), 근섬유아세포 (α-smooth muscle actin) 에대한특이표지가증가된양상을보이는지여부를통해판단할수있다. 후자의논리는제대혈유래줄기세포가창상치유를증가시키는체액인자의분비량을증가시키는지여부를확인함으로써뒷받침될수있다. 본실험의경우에서실험군과대조군의 TGF-β1 의분비가차이가없는것으로나왔으나이비교결과는실험후 6일째시행되었기때문에초기 3일까지의창상치유에중요한인
5 42 대한화상학회지 Vol. 12, No. 1, 2009 자로인식되는 TGF-β1 등의체액인자가큰영향을미치지않는다고생각하기어렵다. 따라서추후 integrin α3, endoglin-cd105, α-smooth muscle actin, 초기 (3일) TGF-β1 발현량등의면밀한실험을통해서창상치유의기전을밝혀야할것으로사료된다. 인체제대혈유래단핵구세포는제대혈유래중간엽줄기세포에비해배양의과정을거치지않기때문에감염 ( 배양액에포함되어있는소태아혈청 (fetal bovine serum) 으로부터인수공통감염등 ) 의가능성이상대적으로적어안전하며빠른적용이가능하므로창상치료에도움이될것으로여겨진다. 결 CD34 단핵구세포는 CD34+ 군에비해높은창상치유율을나타내는것으로보아, 피부창상치유에조혈모세포계열로여겨지는 CD34+ 단핵구세포는큰영향을주지않고중간엽줄기세포를포함하고있는 CD34 단핵구세포가상피화와신생혈관생성을통해창상치유를향상시키는것으로추론할수있다. 론 REFERENCES 1) Szilvassy SJ, Meyerrose TE, Ragland PL, et al. Differential homing and engraftment properties of hematopoietic progenitor cells from murine bone marrow, mobilized peripheral blood and fetal liver. Blood. 2001;98: ) Vaziri H, Dragowska W, Allsopp RC, et al. Evidence for a mitotic clock in human hematopoietic stem cells: loss of telomeric DNA with age. Proc Natl Acad Sci USA. 1994; 91: ) Migliaccio G, Migliaccio AR, Petti S, et al. Human embryonic hemopoiesis. Kinetics of progenitors and precursors underlying the yolk sac-liver transition. J Clin Invest. 1986;78: ) Zanjani ED, Ascensao JL, Tavassoli M, et al. Liver derived fetal hematopoietic stem cells selectively and preferentially home to the fetal bone marrow. Blood. 1993;81: )Rubinstein P, Rosenfield RE, Adamson JW, et al. Stored placental blood for unrelated bone marrow reconstitution. Blood. 1993;81: ) Rocha V, Wagner Jr JE, Sobocinski KA, et al. Graft-versushost disease in children who have received a cord-blood or bone marrow transplant from an HLA-identical sibling. N Engl J Med. 2000;342(25): ) Lee OK, Kuo TK, Chen WM, et al. Isolation of multi-potent mesenchymal stem cells from umbilical cord blood. Blood. 2004;103: ) Mareschi K, Biasin E, Piacibello W, et al. Isolation of human mesenchymal stem cells: bone marrow versus umbilical cord blood. Haematologica. 2001;86(10): ) Erices A, Conget P, Minguell JJ. Mesenchymal progenitor cells in human umbilical cord blood. Br J Haematol. 2000;109(1): ) Badiavas EV, Abedi M, Butmarc J, et al. Participation of bone marrow derived cells in cutaneous wound healing. J Cell Physiol. 2003;196: ) Badiavas EV, Falanga V. Treatment of chronic wounds with bone marrow-derived cells. Arch Dermatol. 2003;139: ) Yamaguchi Y, Yoshida S, Sumikawa Y, et al. Rapid healing of intractable diabetic foot ulcers with exposed bones following a novel therapy of exposing bone marrow cells and then grafting epidermal sheets. Br J Dermatol. 2004;151: ) Valbonesi M, Giannini G, Migliori F, et al. Cord blood stem cells for wound repair preliminary report of 2 cases. Transfus Apher Sci. 2004;30: ) Yamaguchi Y, Kubo T, Murakami T, et al. Bone marrow cells differentiate into wound myofibroblasts and accelerate the healing of wounds with exposed bones when combined with an occlusive dressing. B J Dermato. 2005;152: ) Volk SW, Antoneta R, Zhang L, et al. Stromal progenitor cell therapy corrects the wound healing defect in the ischemic rabbit ear model of chronic wound repair. Wound Rep Reg. 2007;15:
Kaes010.hwp
갑상선자극호르몬이갑상선암세포의 VEGF, 신생혈관형성, 성장, 침윤및전이에미치는영향 Effects of TSH on the Production of VEGF, Angiogenesis, Growth, Invasion and Metastasis in Thyroid Cancer Cells Euy Young Soh, M.D., Hee Boong Park, M.D.,
More information( )Kju269.hwp
만성세균성전립선염모델흰쥐에서 의항염효과 Anti-inflammatory Effect of Lycopene on Chronic Bacterial Prostatitis Rat Model Cho Hwan Yang, Dong Wan Sohn, Yong-Hyun Cho From the Department of Urology, The Catholic University
More information황지웅
Comparison of Laparoscopy and Exploration in the Distal Pancreatectomy BACKGROUND: To determine the benefits of laparoscopic surgery compared with exploration, the clinical outcomes of open and laparoscopic
More informationJksvs019(8-15).hwp
Grade I Grade II Grade III 12 대한혈관외과학회지 : 제 20 권 제 1 호 2004 Control Group A Group B Fig. 4. Microscopic findings of vein wall in control, group A and group B on the day of 7 after venous occlusion. The
More information(01) hwp
Journal of Life Science 2013 Vol. 23. No. 2. 157~166 ISSN (Print) 1225-9918 ISSN (Online) 2287-3406 DOI : http://dx.doi.org/10.5352/jls.2013.23.2.157 α μ δ κ 158 생명과학회지 2013, Vol. 23. No. 2 Journal of
More informationLumbar spine
Lumbar spine CT 32 111 DOI : 10.3831/KPI.2010.13.2.111 Lumbar Spine CT 32 Received : 10. 05. 23 Revised : 10. 06. 04 Accepted : 10. 06. 11 Key Words: Disc herniation, CT scan, Clinical analysis The Clinical
More information( )Kjhps043.hwp
Difference of Fistula Maturation Degree and Physical Property by the Types of Tube Material: An Experimental Study Sang Koo Kang, M.D. 1, Hee Chul Yu, M.D. 1,4, Woo Sung Moon, M.D. 2,4, Ju Hyoung Lee,
More information012임수진
Received : 2012. 11. 27 Reviewed : 2012. 12. 10 Accepted : 2012. 12. 12 A Clinical Study on Effect of Electro-acupuncture Treatment for Low Back Pain and Radicular Pain in Patients Diagnosed with Lumbar
More information388 The Korean Journal of Hepatology : Vol. 6. No COMMENT 1. (dysplastic nodule) (adenomatous hyperplasia, AH), (macroregenerative nodule, MR
6 3 2000 ; 387-392 (3) Dysplastic Nodule Young Nyun Park, M.D., Chanil Park, M.D. Department of Pathology, Yonsei University College of Medicine BRIEF HISTORY 56. AST/ALT 72/73 IU/L, total bilirubin 0.7
More information<283732372D3733312920B4D9C3CAC1A120BCD2C7C1C6AEC4DCC5C3C6AEB7BBC1EEC0C720B3EBBEC8C0C720BDC3B7C2BAB8C1A4BFA120B4EBC7D120C0AFBFEBBCBA20C6F2B0A1283035292E687770>
대한안과학회지 제 49 권 제 5 호 2008 J Korean Ophthalmol Soc 49(5):727-731, 2008 DOI : 10.3341/jkos.2008.49.5.727 다초점 소프트콘택트렌즈의 노안의 시력보정에 대한 유용성 평가 김현경 1 김효명 2 정성근 1 가톨릭대학교 의과대학 성모병원 안과학교실 1, 고려대학교 의과대학 안암병원 안과학교실
More informationCan032.hwp
Chromosomal Alterations in Hepatocellular Carcinoma Cell Lines Detected by Comparative Genomic Hybridization Sang Jin Park 1, Mahn Joon Ha, Ph.D. 1, Hugh Chul Kim, M.D. 2 and Hyon Ju Kim, M.D. 1 1 Laboratory
More informationγ
경락경혈학회지 Vol.27, No.1, pp.87 106, 2010 Journal of Meridian & Acupoint Dept. of 1 Meridian & Acupoint, 3 Acupuncture & Moxibustion, College of Oriental Medicine, Daejeon University 2 Division of Clinical
More information590호(01-11)
T H E K O R E A N D O C T O R S W E E K L Y www.docdocdoc.co.kr I N S I D E 38 3 5 6 7 10 13 28 29 30 31 38 41 42 2 Advertisement Government & Medicine 3 Government & Medicine 4 Government & Medicine Government
More information139~144 ¿À°ø¾àħ
2 139 DOI : 10.3831/KPI.2010.13.2.139 2 Received : 10. 04. 08 Revised : 10. 04. 26 Two Case Report on Wrist Ganglion Treated with Scolopendrid Pharmacopuncture Accepted : 10. 05. 04 Key Words: Wrist Ganglion,
More information김범수
Analysis of Outcomes after Resection of Sarcomatous Hepatocellular Carcinoma Purpose: Sarcomatous hepatocellular carcinoma (HCC) is rare. Therefore, the clinicopathologic characteristics and prognosis
More information1..
Volume 12, Number 1, 6~16, Factors influencing consultation time and waiting time of ambulatory patients in a tertiary teaching hospital Jee-In Hwang College of Nursing Science, Kyung Hee University :
More information- i - - ii - - iii - - iv - - v - - 1 - - 2 - - 3 - - 4 - - 5 - - 6 - - 7 - - 8 - - 9 - - 10 - - 11 - - 12 - - 13 - - 14 - - 15 - - 16 - - 17 - - 18 - - 19 - α α - 20 - α α α α α α - 21 - - 22 - - 23 -
More information975_983 특집-한규철, 정원호
Focused Issue of This Month Gyu Cheol an, MD Department of Otolaryngology ead & Neck Surgery, Gachon University of College Medicine E - mail : han@gilhospital.com Won-o Jung, MD Department of Otolaryngology
More informationJkbcs016(92-97).hwp
Expression of bcl-2 and Apoptosis and Its Relationship to Clinicopathological Prognostic Factors in Breast Cancer - A Study with Long Term Follow-up correlated with the survival rate.(journal of Korean
More information16(1)-3(국문)(p.40-45).fm
w wz 16«1y Kor. J. Clin. Pharm., Vol. 16, No. 1. 2006 x w$btf3fqpsu'psn û w m w Department of Statistics, Chonnam National University Eunsik Park College of Natural Sciences, Chonnam National University
More information<35BFCFBCBA2E687770>
주요개념 : 컴퓨터음란물 접촉자, 성지식, 성태도 남자중학생의 컴퓨터음란물 접촉자와 비접촉자간의 성지식과 태도의 차이 김 영 혜* 이 화 자** 정 향 미*** 1. 연구의 필요성 한국은 I. 서 론 1960 년대 이후부터 시작된 돌진적 산업화 ( 한 상진, 1996) 로 인하여 경제적 부흥을 이루어 집집마다 TV 수상기를 가질 수 있게 되었고 최근에는 PC보급율
More information54 한국교육문제연구제 27 권 2 호, I. 1.,,,,,,, (, 1998). 14.2% 16.2% (, ), OECD (, ) % (, )., 2, 3. 3
27 2, 53-70, 2009. (Nursing Home) * ** (Nursing Home). 5 50 5 2 1,,,,, SPSS t.,,,,,,,. :,, : 2009/08/27 : 2009/09/24 : 2009/09/30 * ** (: hjkim@daelim.ac.kr) 54 한국교육문제연구제 27 권 2 호, 2009. I. 1.,,,,,,, (,
More information- i - - ii - - iii - - iv - - v - - vi - - 1 - - 2 - - 3 - 1) 통계청고시제 2010-150 호 (2010.7.6 개정, 2011.1.1 시행 ) - 4 - 요양급여의적용기준및방법에관한세부사항에따른골밀도검사기준 (2007 년 11 월 1 일시행 ) - 5 - - 6 - - 7 - - 8 - - 9 - - 10 -
More information이가이드라인은세포치료제의주성분인세포의명명에대한원칙에대하 여식품의약품안전처의입장을기술한것으로대외적으로법적효력을가지 는것이아님을알려드립니다. 가이드라인 이란대외적으로특정한사안등에대하여식품의약품안 전처의입장을기술한것임 ( 식품의약품안전처지침등의관리에관한 규정제 2 조 ( 식
가이드라인관리번호 B1-2017-3-002 세포치료제원료의약품명명법가이드라인 (Guideline for Nomenclature of Drug Substance of Cell Therapy Products) 2017. 03. 31. 바이오생약심사부세포유전자치료제과 이가이드라인은세포치료제의주성분인세포의명명에대한원칙에대하 여식품의약품안전처의입장을기술한것으로대외적으로법적효력을가지
More informationECG & EP CASES Young-Keun On, MD, PhD Division of Cardiology, Department of Medicine Cardiac & Vascular Center, Samsung Medical Center Sungkyunkwan University School of Medicine, Seoul, Korea A case of
More informationÀÇÇа�ÁÂc00Ì»óÀÏ˘
Common Allergic Diseases in Children Sang - Il Lee, M.D. Department of pediatrics Sungkyunkwan University School of Medicine, Samsung Medical Center E - mail : silee@smc.samsung.co.kr Abstract Allergy
More information현대패션의 로맨틱 이미지에 관한 연구
한지닥섬유제품의인체생리반응및쾌적성평가 임순 Evaluation of Thermal Physiological Responses and Comfort in Dox Fabric Soon Im Professor, Dept. of Fashion Industry, Incheon National University This study performed the evaluation
More information석사논문.PDF
ABO Rh A study on the importance of ABO and Rh blood groups information in Public Health 2000 2 1 ABO Rh A study on the importance of ABO and Rh blood groups information in Public Health 2000 2 2 ABO Rh
More informationJournal of Educational Innovation Research 2018, Vol. 28, No. 4, pp DOI: * A Research Trend
Journal of Educational Innovation Research 2018, Vol. 28, No. 4, pp.295-318 DOI: http://dx.doi.org/10.21024/pnuedi.28.4.201812.295 * A Research Trend on the Studies related to Parents of Adults with Disabilities
More informationÀå¾Ö¿Í°í¿ë ³»Áö
Disability & Employment 55 Disability & Employment 56 57 Disability & Employment 58 59 Disability & Employment 60 61 Disability & Employment 62 63 Disability & Employment 64 65 Disability & Employment
More information03-ÀÌÁ¦Çö
25 3 (2004 9 ) J Korean Oriental Med 2004;25(3):20-31 1), 2), 3) 1) 2) 3) Grope for a Summary Program about Intellectual Property Protection of Traditional Knowledge (TK)etc. Discussed in WIPO Hwan-Soo
More information한국성인에서초기황반변성질환과 연관된위험요인연구
한국성인에서초기황반변성질환과 연관된위험요인연구 한국성인에서초기황반변성질환과 연관된위험요인연구 - - i - - i - - ii - - iii - - iv - χ - v - - vi - - 1 - - 2 - - 3 - - 4 - 그림 1. 연구대상자선정도표 - 5 - - 6 - - 7 - - 8 - 그림 2. 연구의틀 χ - 9 - - 10 - - 11 -
More information(
317 318 319 320 1 3 5 5 5 5 2 321 : 1.,,,,, 06 2. X-ray beam penetration (density) (contrast) 03 3. patch coating, precipitation, flaking 03 4. centering 03 5. Esophagus, cardia, fundus, body, angle, antrum,
More informationAbstract Background : Most hospitalized children will experience physical pain as well as psychological distress. Painful procedure can increase anxie
Volume 12, Number 1, 92~102, An Intervention Study of Pain Reduction during IV Therapy in Hospitalized Children Myo-Jin Kim 1), Joung-Hae Bak 1), Won-Seok Seo 2) Mi-Young Kim 3), Sun-Kyoung Park 3), Jai-Soung
More information2009;21(1): (1777) 49 (1800 ),.,,.,, ( ) ( ) 1782., ( ). ( ) 1,... 2,3,4,5.,,, ( ), ( ),. 6,,, ( ), ( ),....,.. (, ) (, )
Abstract Kim Dal-Rae, Kim Sun-Hyung* Dept. of Sasang Constitutional Medicine, College of Oriental Medicine, Kyung-Hee Univ. *Dept. of Sasang Constitutional Medicine, East-West Neo Medical Center, Kyung-Hee
More information2 1896 2 26 ( ) 2 7-20 2) 1897 1898 ( )3) 6 1902 8 1904 10 10 (19 05-1914 ) ( ) ( ) 4) 1915 ( ) ( ) 1917 2) 3) 4) 285
13 2 ( 25 ) 2004 12 Korean J Med Hist 13 284 296 Dec 2004 ISSN 1225 505X 1) * ** ** 1 1920 1930 40 ( 1896-1973) 80 * ** 1) 2003 284 2 1896 2 26 ( ) 2 7-20 2) 1897 1898 ( )3) 6 1902 8 1904 10 10 (19 05-1914
More informationPharmacotherapeutics Application of New Pathogenesis on the Drug Treatment of Diabetes Young Seol Kim, M.D. Department of Endocrinology Kyung Hee Univ
Application of New Pathogenesis on the Drug Treatment of Diabetes Young Seol Kim, M.D. Department of Endocrinology Kyung Hee University College of Medicine & Hospital E mail : ycell2@yahoo.co.kr Abstract
More informationl l l l l l l l l Lee, Geon Kook None This project was designed to establish the Tumor Bank of National Cancer Center in 2000. From the first tumor sample in 2000, the total of tumor and tumor-related
More informationα α α α α
α α α α α α α α 太陰調胃湯加減方 dbdb 마우스 肝에 대한 아디포사이토카인 및 발현에 미치는 영향 SREBPs 와 섞어서 하고 마커 또한 하여 전기 영동을 하였다 전기영동을 한 후에 에 를 쪼여서 각 를 확인하였다 이 를 프로그램을 이용해 수치화하 여 분석하였다 肝 조직 동결 절편 분리한 조직은 로 시간 동안 고정 시킨 후 에 세척한 후 물기를
More information원위부요척골관절질환에서의초음파 유도하스테로이드주사치료의효과 - 후향적 1 년경과관찰연구 - 연세대학교대학원 의학과 남상현
원위부요척골관절질환에서의초음파 유도하스테로이드주사치료의효과 - 후향적 1 년경과관찰연구 - 연세대학교대학원 의학과 남상현 원위부요척골관절질환에서의초음파 유도하스테로이드주사치료의효과 - 후향적 1 년경과관찰연구 - 연세대학교대학원 의학과 남상현 원위부요척골관절질환에서의초음파 유도하스테로이드주사치료의효과 - 후향적 1 년경과관찰연구 - 지도김석원교수 이논문을석사학위논문으로제출함
More informationuntitled
대한수혈학회지 : 제 21 권제 2 호, 2010 냉동보관전제대혈세포의생존율평가 이대영 1 ㆍ허지영 1 ㆍ강명서 1,2 차의과학대학교진단검사의학교실 1, 차병원제대혈은행 2 Assessment of Cell Viability in Umbilical Cord Blood before Cryopreservation Dae Young Yi 1, Ji Young Huh
More informationKor. J. Aesthet. Cosmetol., 및 자아존중감과 스트레스와도 밀접한 관계가 있고, 만족 정도 에 따라 전반적인 생활에도 영향을 미치므로 신체는 갈수록 개 인적, 사회적 차원에서 중요해지고 있다(안희진, 2010). 따라서 외모만족도는 개인의 신체는 타
RESEARCH ARTICLE Kor. J. Aesthet. Cosmetol., 20-40대 여성의 외모만족도가 미용관리태도에 미치는 영향 홍수남 1, 김효숙 2 * 1 건국대학교 뷰티사이언스디자인학과, 2 건국대학교 의상디자인과 Effects of Extrinsic Body Satisfaction on Beauty Management Behavior of
More information7.ƯÁýb71ÎÀ¯È« š
J KMA Special Issue Myelodysplastic Syndrome June Won Cheong, MD Yoo Hong Min, MD Department of Internal Medicine, Yonsei University College of Medicine E mail : jwcheong70@yumc.yonsei.ac.kr minbrmmd@yumc.yonsei.ac.kr
More information레이아웃 1
i g d e d mod, t d e d e d mod, t e,0 e, n s,0 e,n e,0 Division of Workers' Compensation (2009). Iowa workers' compensation manual. Gamber, E. N. & Sorensen, R. L. (1994). Are net discount rates stationary?:
More information( )Jkstro011.hwp
비인강암의방사선치료결과및생존율에관한예후인자분석 2005 2 1 2005 3 28. :, Tel: 053)250-7665, Fax: 053)250-7984 E-mail: jhkim@dsmc.or.kr 정영연외 2 인 : 비인강암의예후인자분석 정영연외 2 인 : 비인강암의예후인자분석 Carcinoma of the nasopharynx treated by radiotherapy
More informationJournal of Educational Innovation Research 2018, Vol. 28, No. 4, pp DOI: 3 * The Effect of H
Journal of Educational Innovation Research 2018, Vol. 28, No. 4, pp.577-601 DOI: http://dx.doi.org/10.21024/pnuedi.28.4.201812.577 3 * The Effect of Home-based Activities Using Traditional Fairy Tales
More information04_이근원_21~27.hwp
1) KIGAS Vol. 16, No. 5, pp 21~27, 2012 (Journal of the Korean Institute of Gas) http://dx.doi.org/10.7842/kigas.2012.16.5.21 실험실의 사례 분석에 관한 연구 이근원 이정석 한국산업안전보건공단 산업안전보건연구원 (2012년 9월 5일 투고, 2012년 10월 19일
More information한국 출산력의 저하 요인에 관한 연구
가족계획실천율 가족계획실천자 세 유배우부인 조출생률 년간 총출생아수 연앙인구수 일반출산율 년간 총출생아수 가임연령 여자연앙인구수 합계출산율 세 연령층 여성이 출산한 출생아수 세 연령층 여성의 연앙인구수 즉 합계출산율 합 6 계 출 산 율 5 4 3 2 1 1960 1973 1977 1981 1985 1989 1993 1997 2001
More informationTHE JOURNAL OF KOREAN INSTITUTE OF ELECTROMAGNETIC ENGINEERING AND SCIENCE Nov.; 26(11),
THE JOURNAL OF KOREAN INSTITUTE OF ELECTROMAGNETIC ENGINEERING AND SCIENCE. 2015 Nov.; 26(11), 985991. http://dx.doi.org/10.5515/kjkiees.2015.26.11.985 ISSN 1226-3133 (Print)ISSN 2288-226X (Online) Analysis
More information,,,.,,,, (, 2013).,.,, (,, 2011). (, 2007;, 2008), (, 2005;,, 2007).,, (,, 2010;, 2010), (2012),,,.. (, 2011:,, 2012). (2007) 26%., (,,, 2011;, 2006;
,,.. 400,,,,,,.,,, -, -, -., 3.. :, Tel : 010-9540-0640, E-mail : sunney05@hanmail.net ,,,.,,,, (, 2013).,.,, (,, 2011). (, 2007;, 2008), (, 2005;,, 2007).,, (,, 2010;, 2010), (2012),,,.. (, 2011:,, 2012).
More information44-4대지.07이영희532~
A Spatial Location Analysis of the First Shops of Foodservice Franchise in Seoul Metropolitan City Younghee Lee* 1 1 (R) 0 16 1 15 64 1 Abstract The foodservice franchise is preferred by the founders who
More informationJournal of Educational Innovation Research 2017, Vol. 27, No. 2, pp DOI: : Researc
Journal of Educational Innovation Research 2017, Vol. 27, No. 2, pp.251-273 DOI: http://dx.doi.org/10.21024/pnuedi.27.2.201706.251 : 1997 2005 Research Trend Analysis on the Korean Alternative Education
More information386-390.hwp
386 HANYANG MEDICAL REVIEWS Vol. 29 No. 4, 2009 우리나라 미숙아의 통계와 의료비용 Statistics and Medical Cost of Preterm in Korea 윤혜선 을지대학교 노원을지병원 소아청소년과학교실 Hye Sun Yoon, M.D., Ph.D., Department of Pediatrics, Nowon
More informationA 617
Special Issue Diabetic Retinopathy Won Ki Lee, M.D. Department of Ophthalmology The Catholic University of Korea College of Medicine Kangnam St. Mary s Hospital E mail : wklee@catholic.ac.kr Abstract R
More informationCrt114( ).hwp
cdna Microarray Experiment: Design Issues in Early Stage and the Need of Normalization Byung Soo Kim, Ph.D. 1, Sunho Lee, Ph.D. 2, Sun Young Rha, M.D., Ph.D. 3,4 and Hyun Cheol Chung, M.D., Ph.D. 3,4 1
More information01-01서상원-전화교정
대한외상학회지 Vol. 20, No. 1, June, 2007 원 저 동종유래각질세포 (Cultured Allogenic Keratinocytes, Kaloderm ) 를이용한부분층피부결손의치료 성균관대학교의과대학강북삼성병원성형외과학교실, 테고사이언스ㄜ * 서상원 장충현 조민수 홍윤기 전세화 * Abstract Treatment of Partial Thickness
More information歯제7권1호(최종편집).PDF
********* (*, **, *** ).., 2002, 7, 1, 1-12. 2-5 80.,.,..,,... :,,. (naming).., (word finding), (lexical look- up), (lexical retrieval), (word recall) (Fried- Oken, 1987). (pause),,, (naming error) (Snyder
More information달생산이 초산모 분만시간에 미치는 영향 Ⅰ. 서 론 Ⅱ. 연구대상 및 방법 達 은 23) 의 丹 溪 에 최초로 기 재된 처방으로, 에 복용하면 한 다하여 난산의 예방과 및, 등에 널리 활용되어 왔다. 達 은 이 毒 하고 는 甘 苦 하여 氣, 氣 寬,, 結 의 효능이 있
대한한방부인과학회지 THE JOURNAL OF ORIENTAL OBSTETRICS & GYNECOLOGY VOL.17, NO.2 : 115-122 (2004) 달생산이 초산모 분만시간에 미치는 영향 * 북경한의원, ** 윤산부인과의원, *** 최은림산부인과의원, 상지대학교 한의과대학 부인과학교실 ****, 경희대학교 동서의학대학원 김성준 *****, 윤왕준
More information기관고유연구사업결과보고
기관고유연구사업결과보고 작성요령 2001 ~ 2004 2005 ~ 2007 2008 ~ 2010 2001 ~ 2004 2005 ~ 2007 2008 ~ 2010 1 2/3 2 1 0 2 3 52 0 31 83 12 6 3 21 593 404 304 1,301 4 3 1 8 159 191 116 466 6 11 (`1: (1: 16 33 44 106
More information노영남
Purpose: Delayed massive hemorrhages from pseudoaneurysm rupture of the peripancreatic large arteries, after pancreaticoduodenectomy, are fatal. We reviewed the clinical course and outcome of bleeding
More information00약제부봄호c03逞풚
경희대학교 동서신의학병원 약품 정보지 2 0 0 7. S P R I N G. V O L. 0 1 신약 소개 02 Journal Review 03 Special Subject 04 복약 지도 06 의약품 안전성 정보 07 약제부 알림 07 약제부 업무 소개 08 E A S T - W E S T N E O M E D I C A L C E N T E R 본 약품
More informationDBPIA-NURIMEDIA
김진주 김수연. 초등학생대상장애이해교육에활용된동화에나타난장애인관분석. 특수교육, 2013, 제12권, 제2호, 135-160... 20.,,. 4.,,.,..... 주제어 : 장애이해교육, 동화, 장애인관 1. ( 1 ) Incheon Munhak Elementary School ( )(, E-mail: sooyoun@ginue.ac.kr) Dept. of
More informationJournal of Educational Innovation Research 2019, Vol. 29, No. 2, pp DOI: 3 * Effects of 9th
Journal of Educational Innovation Research 2019, Vol. 29, No. 2, pp.357-378 DOI: http://dx.doi.org/10.21024/pnuedi.29.2.201906.357 3 * Effects of 9th Grade Students Participation in Career Curriculum Cluster
More informationAbstract Musculoskeletal Symptoms and Related Factors for Nurses and Radiological Technologists Wearing a Lead Apron for Radiation Pro t e c t i o n Jung-Im Yoo, Jung-Wan Koo 1 ) Angio Unit, Team of Radiology,
More informationJkbcs032.hwp
Clinical Correlation of HER-2/neu Overexpression in Patients with Breast Cancer Sung Yong Kim, Tae Yoon Kim, Jae Jun Kim, Chang Ho Kim, Ok Pyung Song, Min Hyuk Lee, Eui Han Kim 1, and Moo Sik Cho Departments
More information09구자용(489~500)
The Study on the Grid Size Regarding Spatial Interpolation for Local Climate Maps* Cha Yong Ku** Young Ho Shin*** Jae-Won Lee**** Hee-Soo Kim*****.,...,,,, Abstract : Recent global warming and abnormal
More information:,,.,. 456, 253 ( 89, 164 ), 203 ( 44, 159 ). Cronbach α= ,.,,..,,,.,. :,, ( )
:,,.,. 456, 253 ( 89, 164 ), 203 ( 44, 159 ). Cronbach α=.83.91.,.,,..,,,.,. :,, (402-751) 253 E-mail : yspark@inha.ac.kr ..,.,.,. Bandura(1997),.,,,,., (,,, 2009), (,,,, 2014).?.,,,.. Bandura (1995) (Resiliency
More information대한한의학원전학회지26권4호-교정본(1125).hwp
http://www.wonjeon.org http://dx.doi.org/10.14369/skmc.2013.26.4.267 熱入血室證에 대한 小考 1 2 慶熙大學校大學校 韓醫學科大學 原典學敎室 韓醫學古典硏究所 白裕相1, 2 *117) A Study on the Pattern of 'Heat Entering The Blood Chamber' 1, Baik 1
More information10(3)-09.fm
w y wz 10«3y 253~258 (2010.12.) Journal of Korean Society of Urban Environment ³ w Á» Á Á y w y œw (2010 11 22, 2010 12 9 k) Study on Determine of Detention Pond in Small Developed Area In-Soo Chang ½
More information12¾ÈÇö°æ1-155T304®¶ó153-154
Journal of Fashion Business Vol. 8, No. 4, pp.141~155(2004) A Research for the hair Style Image making Chart Manufacturing Depends on Fashion Feeling + - through the analysis of the actress hair styles
More information대한한의학원전학회지24권6호-전체최종.hwp
小兒藥證直訣 의 五臟辨證에 대한 小考 - 病證과 處方을 중심으로 1 2 慶熙大學校大學校 韓醫學科大學 原典學敎室 ㆍ 韓醫學古典硏究所 白裕相1,2*1)2) A study on The Diagnosis and Treatment Using The Theory of Five Organs in Soayakjeungjikgyeol(小兒藥證直訣) 1 Dept. of Oriental
More information歯kjmh2004v13n1.PDF
13 1 ( 24 ) 2004 6 Korean J Med Hist 13 1 19 Jun 2004 ISSN 1225 505X 1) * * 1 ( ) 2) 3) 4) * 1) ( ) 3 2) 7 1 3) 2 1 13 1 ( 24 ) 2004 6 5) ( ) ( ) 2 1 ( ) 2 3 2 4) ( ) 6 7 5) - 2003 23 144-166 2 2 1) 6)
More information서론 34 2
34 2 Journal of the Korean Society of Health Information and Health Statistics Volume 34, Number 2, 2009, pp. 165 176 165 진은희 A Study on Health related Action Rates of Dietary Guidelines and Pattern of
More informationA Study on the Relationships between Self-Differentiation and Adaptability Factors for Senior Dementia Patients Care Givers Department of Social Welfare, Seoul Welfare Graduate University Full-time Lecturer
More information목차 1. 서론 줄기세포의간세포분화능평가시고려사항 간세포분화능평가시험법 분
세포치료제시험정보집 7 줄기세포치료제의 간세포분화능평가시험 2015. 4. 30. 식품의약품안전평가원 차세대줄기세포기반제제평가연구사업단 목차 1. 서론 --------------------------------------------- 1 2. 줄기세포의간세포분화능평가시고려사항 --------------- 2 3. 간세포분화능평가시험법 -----------------------------
More informationTrd022.hwp
김신태, 이선녕, 이석정, 정필문, 박홍준, 신명상, 김종환, 이부길, 김상하, 리원연, 신계철, 용석중 Shin-Tae Kim, M.D., Shun Nyung Lee, M.D., Seok Jeong Lee, M.D., Pil Moon Jung, M.D., Hong Jun Park, M.D., Myung Sang Shin, M.D., Chong Whan Kim,
More information충북의대학술지 Chungbuk Med. J. Vol. 27. No. 1. 1~ Charcot-Marie-Tooth Disease 환자의마취 : 증례보고 신일동 1, 이진희 1, 박상희 1,2 * 책임저자 : 박상희, 충북청주시서원구충대로 1 번지, 충북대학교
충북의대학술지 Chungbuk Med. J. Vol. 27. No. 1. 1~5 2017 Charcot-Marie-Tooth Disease 환자의마취 : 증례보고 신일동 1, 이진희 1, 박상희 1,2 * 책임저자 : 박상희, 충북청주시서원구충대로 1 번지, 충북대학교의과대학마취통증의학교실, 의학연구소 (28644), happycat19@hanmail.net
More information605.fm
Journal of the Korean Housing Association Vol. 19, No. 6, 2008 y k y p w sƒ Care-giver s Needs and Evaluation on the Actual Condition of the Playgrounds in Child Care Facilities y* Choi, Mock-Wha x **
More informationuntitled
대한골다공증학회지 : 제 3 권제 1 호 pp 1~6, 2005 종설 성인중간엽줄기세포의계대배양에따른특성변화 연세대학교의과대학정형외과학교실 1, 연세대학교 BK21 의과학과 2 김윤희 2 선현진 2 한승환 1 이진우 1,2 Characterization of Human Mesenchymal Stem Cells during Serial Subculture Yun
More information자기공명영상장치(MRI) 자장세기에 따른 MRI 품질관리 영상검사의 개별항목점수 실태조사 A B Fig. 1. High-contrast spatial resolution in phantom test. A. Slice 1 with three sets of hole arr
Original Article pissn 1738-2637 J Korean Soc Radiol 2012;67(2):129-134 The Survey of Magnetic Resonance Imaging Quality according to in Korea 1 자기공명영상장치(MRI) 자장세기에 따른 MRI 품질관리 영상검사의 개별항목점수 실태조사 1 Hyun-Hae
More informationA Study on Married Female Immigrants Life Style and Marriage Satisfaction in Terms of Preparing Their Old Age in Chungcheongnam-do Department of Gerontology, Hoseo University Doctoral Student : Hi Ran
More information분 기 보 고 서 (제 47 기) 사업연도 2014년 01월 01일 2014년 03월 31일 부터 까지 금융위원회 한국거래소 귀중 2014년 5월 30일 제출대상법인 유형 : 면제사유발생 : 주권상장법인 해당사항 없음 회 사 명 : 파미셀 주식회사 대 표 이 사 : 김
목 분 기 보 고 서...1 대표이사 등의 확인...2 I. 회사의 개요...3 1. 회사의 개요...3 2. 회사의 연혁...4 3. 자본금 변동사항...7 4. 주식의 총수 등...9 5. 의결권 현황...10 6. 배당에 관한 사항 등...10 II. 사업의 내용...12 III. 재무에 관한 사항...30 IV. 감사인의 감사의견 등...34 V. 이사의
More informationuntitled
대한수혈학회지 : 제 20 권제 1 호, 2009 제대혈특성에영향을주는산모및태아측요인분석 이혜련 1 ㆍ노은연 2,4 ㆍ윤종현 1,2,4 ㆍ한규섭 1,2,4 ㆍ김병재 3,4 ㆍ황규리 3 ㆍ전혜원 3 ㆍ홍정자 4 ㆍ신수 1,2,4 서울대학교의과대학검사의학교실 1, 보라매병원진단검사의학과 2, 산부인과 3, 서울특별시보라매병원공여제대혈은행 4 = Abstract
More information서론
- i - - ii - - iii - - iv - - v - - vi - - 1 - - 2 - - 3 - - 4 - - 5 - - 6 - - 7 - - 8 - - 9 - - 10 - - 11 - - 12 - - 13 - - 14 - - 15 - - 16 - - 17 - - 18 - - 19 - - 20 - - 21 - - 22 - - 23 - - 24 - -
More informationJournal of Educational Innovation Research 2019, Vol. 29, No. 1, pp DOI: * Suggestions of Ways
Journal of Educational Innovation Research 2019, Vol. 29, No. 1, pp.65-89 DOI: http://dx.doi.org/10.21024/pnuedi.29.1.201903.65 * Suggestions of Ways to Improve Teaching Practicum Based on the Experiences
More informationV V V α Azam, M. Z. M. & Park, Dong-Ho (2012). The relationship between Digit Ratio(2D:4D) and Physical Fitness in Children. Journal of Korean Society of Living Environmental System, 19(1), 101-110.
More informationÆ÷Àå82š
Lee, Kyungbae (E-mail : kblee6078@ex.co.kr) Lee, Jaehoon (E-mail : ranian74@ex.co.kr) Sohn, Duecksu (E-mail : mgsds@ex.co.kr) Kwon, Soonmin (E-mail : soonmini2@ex.co.kr) ABSTRACT PURPOSES : The purpose
More information<BFACBCBCC0C7BBE7C7D02831302031203139292E687770>
延 世 醫 史 學 제12권 제2호: 29-40, 2009년 12월 Yonsei J Med Hist 12(2): 29-40, 2009 특집논문 3 한국사회의 낙태에 대한 인식변화 이 현 숙 이화여대 한국문화연구원 1. 들어가며 1998년 내가 나이 마흔에 예기치 않은 임신을 하게 되었을 때, 내 주변 사람들은 모두 들 너무나도 쉽게 나에게 임신중절을 권하였다.
More information±èÇ¥³â
533 Fig. 1. Dead zone. Nine echoes are positioned 2-10 mm below the scan surface with 1 mm distance. All nine echoes are clearly visualized. 534 Fig. 2. Vertical and horizontal measurement. 10 cm distance
More information( )Kju225.hwp
비임균성비뇨생식기감염에서 의진단적효용성 Usefulness of the Mycofast Test (MYCOFAST Evolution 2) for the Diagnosis of Nongonococcal Genitourinary Infections Hang Ro Park, Yang Hyun Kim, Ho Jae Lee, Jea Sang Oh, Hyoung Jin
More informationJournal of Educational Innovation Research 2017, Vol. 27, No. 4, pp DOI: * A Study on Teache
Journal of Educational Innovation Research 2017, Vol. 27, No. 4, pp.149-171 DOI: http://dx.doi.org/10.21024/pnuedi.27.4.201712.149 * A Study on Teachers and Parents Perceptions on the Introduction of Innovational
More information44-3대지.02이광률c
Properties of Channel and Evolutions of Fluvial Terraces in Odae River Gwang-Ryul Lee* 1 3 OSL 1 MIS 2 2 MIS 3 2 0 205m/ka 0 269m/ka Abstract This study analyzes the properties of fluvial landforms in
More information7 1 ( 12 ) 1998 ( ) ( ) ( ) ( ) ( ). ( ) ( ). ( ) ( ) ( ). ( ). ( ) ( ) ( ) 3).., 16 ( ) ( ) ( ) ( ) ( ) ( ) ( ). 4). ( ) ( ) ( ),., 4-5, 6-7, 5) 6) (
7 1 ( 12 ) : 75-96, 1998 K orean J M ed H ist 7 : 75-96, 1998 ISSN 1225-505X (2)* ** *** ** 1. (4) 2. (5) (1) (6) (2) (7) (3) 5. 3. (1) ( ) 4. ( ) (2) (1) (3) (2). ( ) (3) 6. 4. ( ) 6 1). ( ), ( ).. (1)
More informationuntitled
대한마취과학회지 2008; 55: 190~6 Korean J Anesthesiol Vol. 55, No. 2, August, 2008 임상연구 대한민국의 마취통증의학 임상실습 현황 연세대학교 의과대학 마취통증의학교실 및 마취통증의학연구소 장동진ㆍ안소운ㆍ안지원ㆍ김종훈 The current status of anesthesiology clerkship in Korea
More information07-1960(JH)
한국두피모발미용학회지 제5권 제1호 2009 The Journal of Beauty & Trichology 5(1), 00~00(2008) 1960년대 뷰티 및 패션트렌드 분석 - 모델 트위기 (Twiggy) 를 중심으로 - 김희선* 안양과학대학 뷰티디자인학부 Analysis of Beauty and Fashion trends in the 1960 s - Focusing
More information04조남훈
Received : 2011. 11. 16 Reviewed : 2011. 11. 25 Accpeted : 2011. 12. 5 A Case Report of Prescribing Yanghyeolgeopung-tang(yangxuequfeng-tang) to Two Patients with Cervical Disc Herniation and Headache
More information- iii - - i - - ii - - iii - 국문요약 종합병원남자간호사가지각하는조직공정성 사회정체성과 조직시민행동과의관계 - iv - - v - - 1 - - 2 - - 3 - - 4 - - 5 - - 6 - - 7 - - 8 - - 9 - - 10 - - 11 - - 12 - - 13 - - 14 - α α α α - 15 - α α α α α α
More information<31382D322D3420BDC5B1D4C8AF5FB3EDB9AE28C3D6C1BEBABB292E687770>
醫 史 學 제18권 제2호(통권 제35호) 2009년 12월 Korean J Med Hist 18ː57 72 18ː173-188 Dec. Dec. 2009 2009 C 大 韓 醫 史 學 會 ISSN ISSN 1225 505X 1225-505X 지방병 연구와 식민지배 : 1927년 영흥 및 해남지역 에메틴 중독사건을 중심으로 신 규 환* 1. 머리말 2. 일제의
More information부속
04 Definitions of terminology in neonatal, infant, and perinatal epidemiology.,. (perinatal period),,,, (morbidity) (mortality).,,,.,,,.,., 2. (World Health Organization, WHO) International Statistical
More information44-3대지.08류주현c
A Comparative Study on the Commuting Regional Type According to the Features of Foreigner Commuting Ju-Hyun Ryu* Abstract The purposes of this study are to identify many implications of commuting regional
More information