학술연구용역사업최종결과보고서 신종인플루엔자바이러스유전정보분석 DB 구축및바이러스 항원구조기반수용체결합특이성분석 Database construction for the analyses of genetic information and structure-based receptor specificity study for new influenza viruses 주관연구기관 : 고려대학교산학협력단 질병관리본부
신종인플루엔자바이러스유전정보분석 D B 구축및바이러스항원구조기반수용체결합특이성분석 2 0 1 0 질병관리본부
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목 차 Ⅰ. 연구개발결과요약문 ( 한글 ) 신종인플루엔자바이러스유전정보분석 DB 구축및바이러스항원구조기반수용체결합특이성분석 ( 영문 ) Database construction for the analyses of genetic information and structure-based receptor specificity study for new influenza viruses Ⅱ. 학술연구용역사업연구결과 제1장최종연구개발목표 ------------------------------------------------------ 3 1. 목표 2. 목표달성도 3. 국내 외기술개발현황 제 2 장최종연구개발내용및방법 ----------------------------------------------- 13 1. 연구내용및범위 2. 연구방법 제 3 장최종연구개발결과 ---------------------------------------------------- 17 1. 연구결과 I 2. 연구결과 II 제 4 장연구결과고찰및결론 -------------------------------------------------- 34 제 5 장연구성과 ------------------------------------------------------------ 39 1. 활용성과 2. 활용계획 제 6 장기타중요변경사항 ------------------------------------------------------ 42 제 7 장참고문헌 ------------------------------------------------------------ 43 제 8 장첨부서류 ------------------------------------------------------------ 44-4 -
과제명 중심단어 신종인플루엔자바이러스유전정보분석 DB 구축및바이러스항원구조기반 수용체결합특이성분석 신종인플루엔자바이러스, 유전자와단백질서열, 단백질 3 차구조, 수용체 주관연구기관고려대학교산학협력단주관연구책임자김경현 연구기간 2009. 8. 21-2010. 6. 20-5 -
Title of Project Database construction for the analyses of genetic information and structure-based receptor specificity study for new influenza viruses Key Words 2009 A(H1N1) influenza virus, gene and protein sequence, protein 3D structure, receptor Institute Industry academic cooperation foundation, Korea University Project Leader Kyung Hyun Kim Project Period 2009. 8. 21-2010. 6. 20-6 -
학술연구용역사업연구결과 - 7 -
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RNA segment/ Protein name Protein size (# of a.a) 1 PB2 759 Protein function Infectivity Occurrence RNA polymerase, nuclear transport virulence transmission E627K in 1918 Reassortment in 2009 H1N1 avian H1N1 2 PB1 757 RNA polymerase virulence PB1-F2 87 Induce apoptosis virulence Full-length in 1918, H5N1 human H3N2 3 PA 716 polymerase complex avian H1N1 4 HA 566 hemagglutinin, receptor binding, membrane fusion virulence transmission Multibasic site in H5N1 swine H1N1 5 NP 498 nucleocapsid swine H1N1 6 NA 454 neuraminidase 7 M1, M2 252, 97 matrix protein, ion channel H275Y in 2009 H1N1 Eurasian swine H1N1 Eurasian swine H1N1 8 NS1, NS2 230, 121 interferon response, cytokine storm (NS1) virulence C-term PDZ motif swine H1N1-9 -
α 가 나 α α α - 10 -
α - 11 -
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연구수행체계 - 13 -
연구개발목표에입각한연구목표의달성도및관련분야연구에의기여도등을기술함. ( : 당초계획, : 진척상황 ) - 14 -
다 - 15 -
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HA - 22 -
Countries Segment Total PB2 PB1/ PB1-F2 PA HA NP NA M1/M2 NS1/NS2 Australia 56 56 57 62 57 62 116 112 578 Brazil 0 0 0 19 0 4 4 0 27 Canada 45 45 45 45 24 45 90 89 428 Chile 0 1 0 2 0 0 0 0 3 China 27 27 27 29 27 28 56 55 276 Colombia 1 1 1 1 1 1 2 2 10 Denmark 2 3 3 4 3 3 5 5 28 Dominican 4 4 4 4 4 4 8 8 40 Republic Finland 2 0 3 3 2 3 6 6 25 France 11 14 9 14 7 11 28 28 122 Germany 5 5 9 10 4 6 18 15 72 Greece 0 0 0 0 0 2 24 0 26 Hong Kong 0 0 0 2 0 1 0 0 3 Israel 2 2 2 1 2 2 6 5 22 Italy 4 4 4 16 4 7 8 8 55 Japan 31 31 32 63 32 63 64 64 380 Kazakhstan 0 0 0 1 0 1 2 0 4 Korea 1 1 1 178 1 192 2 2 378 Luxembourg 1 1 2 1 1 1 1 2 10 Mexico 24 28 25 42 26 38 70 52 305 Myanmar 0 0 0 5 0 5 0 0 10 Netherlands 1 1 1 1 1 1 2 2 10 New Zealand 0 1 1 0 1 0 6 0 9 Nicaragua 0 1 1 1 1 1 2 2 9 Norway 2 0 2 3 2 4 8 4 25 Poland 0 0 0 0 0 5 0 0 5 Portugal 0 0 0 0 0 0 0 2 2 Russia 9 9 9 17 13 14 30 28 129 Singapore 0 0 0 9 0 0 0 0 9 Spain 1 1 1 1 16 0 53 48 121 Sweden 0 0 0 4 0 14 8 23 49 Taiwan 8 8 8 6 7 8 16 16 77 Thailand 6 6 9 6 7 5 16 18 73 Turkey 0 0 0 0 0 0 1 0 1 USA 347 361 358 540 429 538 928 755 9729 United Kingdom 1 1 1 1 1 1 2 2 10 Total 1364 1365 1512 1910 1441 1864 1955 1649 13060-23 -
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A B C A - 28 -
B C A B C - 29 -
μ A B C D - 30 -
A B - 31 -
A B C D - 32 -
A B C - 33 -
A B C - 34 -
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A 3500 3000 2500 2000 1500 1000 500 0-500 -1000 110 90 70 50 30 10-10 B 4480 3980 3480 2980 2480 1980 1480 980 480-20 0 20 40 60 80 100 120 140 C 2950 100 2450 1950 1450 950 450 80 60 40 20-50 0-37 -
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과제명 과제책임자 신종인플루엔자바이러스유전정보분석 DB 구축및바이러스항원구조기반 수용체결합특이성분석 김경현 / 고려대학교 / 생물구조정보학 번호논문제목저자명저널명집 ( 권 ) 페이지 Impact factor 1 2 국내 / 국외 SCI여부 번호발표제목발표형태발표자학회명연월일발표지 2 국내 / 국제 번호출원 / 등록 1 해당사항없음 2 해당사항없음 특허명출원 ( 등록 ) 인출원 ( 등록 ) 국출원 ( 등록 ) 번호 IPC 분류 - 43 -
해당사항없음 해당사항없음 해당사항없음 - 44 -
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1. Meumann G, Noda T, and Kawaoja Y. Emergence and pandemic potential of swine-origin H1N1 influenza virus. Nature (2009) 459, 931-939. 2. Russell RJ, Kerry PS, Stevens SJ, Steinhauer DA, Martin SR, Gamblin SJ, Skehel JJ. Structure of influenza hemagglutinin in complex with an inhibitor of membrane fusion. Proc. Natl. Acad. Sci. USA (2008) 105, 17736-17741. 3. Frase C, Donnelly CA, Cauchemez S. et al. Pandemic potential of a strain of influenza A (H1N1): early findings. Science (2009) 324, 1557-1561 4. Smith GJD, Vijaykrishna D, Samantha JB et al. Origins and evolutionary genomics of the 2009 swine-origin H1N1 influenza A epidemic. Nature (2009) 459, 1122-1125. 5. Ekiert DC, Bhabha G, Elsliger M et al. Antibody recognition of a highly conserved influenza virus epitope. Science (2009) 324, 246-251. 6. Vibould C, Bjornstad ON, SMith DL, Simonsen L, Miller MA, Grenfell BT. Synchrony, waves, and spatial hierarchies in the spread of influenza. Science (2006) 312, 447-451. 7. Ferguson NM, Galvani AP, Bush RM. Ecological and immunological determinants of influenza evolution. Nature (2003) 422, 428-433. 8. Centers for Disease Control and Prevention. Preventing emerging infectious diseases: a strategy for the 21st century (US department of Health and Human Services, Atlanta, 1998). 9. Chi XS, Bolar TV, Zhao P, Tam JS, Rappaport R, Cheng S. Molecular evolution of human influenza A/H3N2 virus in Asia and Europe from 2001 to 2003. J. Clin. Microbiol. (2005) 43, 6130-6132. 10. Li D, Saito R, Le MTQ et al. Genetic analysis of influenza A/H3N2 and A/H1N1 viruses circulating in Vietnam from 2001 to 2006. J. Clin. Microbiol. (2008) 46, 399-405. 11. De Clercq E. Antiviral agenst active against influenza A viruses. Nat. Rev. Drug Discor. (2006) 5, 1015-1025. 12. Shinde, V et al. Triple-Reassortant Swine Influenza A (H1) in Humans in the United States, 2005 2009. New England Journal of Medicine. Online publication May 7, 2009. 13. Dawood, F. S. et al. Emergence of a Novel Swine-Origin Influenza A (H1N1) Virus in Humans. New England Journal of Medicine (2009) Online publication May 7, 2009. 14. Min, J. Y., S. Li, G. C. Sen, and R. M. Krug. 2007. A site on the influenza A virus NS1 protein mediates both inhibition of PKR activation and temporal regulation of viral RNA synthesis. Virology 363:236-43. - 47 -
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