간단하고정확한 SPE 로복잡하고까다로운시료전처리문제해결! Alltech 은고상추출을위한선택의폭이넓은다양한고품질의제품을제조공급한다. Alltech의가장독특한장치중의하나가바로 Maxi-Clean Cartridge 이며 10 가지의서로다른사용방법이있다. 이러한카트리지는까다로

간단하고정확한 SPE 로복잡하고까다로운시료전처리문제해결! Alltech 은고상추출을위한선택의폭이넓은다양한고품질의제품을제조공급한다. Alltech의가장독특한장치중의하나가바로 Maxi-Clean Cartridge 이며 10 가지의서로다른사용방법이있다. 이러한카트리지는까다로운추출이나 SPE를 1. 많은시료는 Vacuum Manifold를이용 Vacuum manifold에다량의 Maxi-Clean Cartridge를미리준비하여시간과용매를절약할수있다. 보다빠른준비를위해이중으로쌓아사용할수있다 이용한수집방법을다루는데사용할수있다. 2. 표준형의 4ml 컬럼을사용하여추출방법을직접대체할수있다. Maxi-Clean Cartridge는확립된추출방법안에서쉽게대체하기위해전통적으로사용된 4ml SPE 컬럼과같은베드용적을가진다. 3. 복잡한분리시연속하여두개의카트리지를쌓아서사용 Maxi-Clean Cartridge는복잡한분석을한단계의추출로하기위해두상을결합하여사용할수있다. 만약원한다면, 각각의상을개별적으로용리할수있다. 4. 추출의용이성실린지를사용하여시료를어디로든지추출할수있다. - 직접시료주입밸브안으로, 또는다른저장용기로! Needle을붙일수도있고셉타또는 96-Well sealing mat를통하여직접추출할수있다. 5. Filter Tube 와실린지여과기를연속연결하여 SPE 와여과기능을결합할수있다. Maxi-Clean Cartridge의 Luer 연결부는실린지여과기, 즉시료를 SPE 베드에넣기전에미립자를제거하기위한프릿이설치된 SPE 튜브와실린지여과기를덧붙여사용할수있다. 6. 쉬운운반과저장 7. 개별또는병렬사용가능 오염이나건조됨이없이시료를담고있는 Maxi-Clean Super Cartridge를이동하거나저장하려면상단과하단캡을사용하여밀봉한다. 시료양이적다면, Vacuum manifold를사용하는대신에실린지를사용하고, 반대로시료의양이많다면, 빈 SPE Tube를 manifold에연결하는방법을선택하십시오! 2

시료전처리과정과노동력의단순화로시간과비용의획기적절감효과! 8. 일직선으로사용 다양한 SPE 제품의응용사례 Maxi-Clean cartridge의 luer 연결부는저압응용에서 Guard 카트리지역할을하거나오염물질을제거하기위해 In-Line으로연결하여사용한다. 9. 고객맞춤형제품공급단지용기의크기만을변화시킴으로써베드용적을바꾸지않고시료의크기에맞는독창적인 Maxi-Clean Cartridge를직접만들어사용할수있다. 실린지에의한 Maxi-Clean 사용법 Vacuum Manifold 에의한 Extract-Clean & Ultra Clean 사용법 10. 모든 SPE 요구를충족하는 23 종의제품을선택할수있다. Alltech은모든추출에필요한고품질의 SPE 흡착제를제작공급한다. 여러분이 Alltech SPE 제품을구입하신다면항상제품에대한품질과신뢰성을보장받을수있습니다. 실린지에의한 Ultra, Extra- Clean 사용법 실린지에의한 Ultra, Extra- Clean 의또다른사용법 올바른 SPE 충진제나또는여러분의응용을위한장치를선택하는데있어더많은정보를얻기를원하신다면 Alltech Website www.alltechweb.com/literature /catalog500/217.pdf and 218.pdf를다운로드하시면보다많은자료를열람할수있습니다. Filter Flask 에의한 Extra-Clean & Ultra- Clean 의사용법 www.dongmyung.co.kr 3

SPE 제품의선택 SPE 제품의올바른선택방법 1. 시료의특성즉, 매트릭스 (Matrix) 와관련된극성도, 하전된작용기의존재유무, 시료의용해도, 분자량등과같은요소들을고려하여 SPE 충진베드 (Bed) 에얼마나강하게시료를머무르게할것인지를결정해야한다. 2. 시료를머무르게하는방법의선택. 두가지방법이가능하다. 방법 1. 원하는성분은충진물을통과시키고간섭물질을머무르게하는방법방법 2. 원하는성분은충진물에흡착시키고그이외의간섭물질은통과시키는방법으로이방법은또한시료가용리되는동안시료를농축시킬수있다. 3. 적합한충진형태와 Bed 크기의선택. 최고의회수율을가진순수한추출물을얻기위해선알맞은충진물을선택해야한다. 충진베드의크기가적합하지않을경우종종추출회수율이떨어진다. 즉, 충진베드가너무크면용리가불완전하고, 충진베드가너무작으면머무름 (Retention) 이불완전해진다. 많은시료중에포함된미지요소들로인해응용을위한최적의충진베드의크기를결정하기위하여때로는예비실험이요구되기도한다. 최적의충진베드의크기를결정하기위한방법으로는먼저 200mg, 또는 500mg과같은중간정도의충진베드로시작한다. 만약완전한머무름이얻어지면, 더작은크기의충진베드와용리부피 (Elution Volume) 를사용할수있다. 반면불완전한머무름을보인다면더큰크기의충진베드와용리부피를사용해야한다. 4. 적당한컨디셔닝, 세척그리고용리용매의선택. 충진물질과관련하여용매의세기를고려해야한다. 최종의컨디셔닝용매는용리용매로작용하지않도록그세기가약해야하며잠재적으로전하를띤화합물의이온화를조절하기위하여완충용액 (Buffer) 을사용해야한다. 세척용매는시료가용리되지않도록충진베드에머물러있는간섭물질을서서히제거해야한다. 용리용매는소량 (1~2ml) 의용매로시료를완전히용리시키기위해용리세기가강한용매를사용한다. SPE Packing Materials Specifications FUNCTIONAL GROUP BASE AVERAGE PARTICLE SIZE PORE SIZE % CARBON ENDCAPPED Reversed Phases (Non-Polar) Octadecyl (C18) Silica 50μm 60A 6.0% Yes High Flow C18 Silica 100μm 60A 8.0% Yes High Capacity C18 Silica 50μm 60A 17.0% Yes Octyl (C8) Silica 50μm 60A 4.5% Yes High Capacity C8 Silica 50μm 60A 8.5% Yes Ethyl (C2) Silica 50μm 60A 5.5% Yes Phenyl (PH) Silica 50μm 60A 3.8% Yes Cyclohexyl (CH) Silica 50μm 60A 3.5% Yes Normal Phases (Polar) Silica (SI) Silica 50μm 60A N/A N/A Aminopropyl (NH₂) Silica 50μm 60A 5.0% No Cyanopropyl (CN) Silica 50μm 60A 6.0% Yes Diol (2OH) Silica 50μm 60A 4.0% No Florisil (FL) Magnesium Silicate 75~150μm 60A N/A N/A Alumina (AL) Aluminum Oxide 130μm 100A N/A N/A Specifications DESCRIPTION BASE PARTICLE SIZE RETAINS Miscellaneous Packings DVB Ultra-Pure Divinylbenzene 40μm Hydrophobic Compounds Carbograph Graphitized Carbon 38-125μm Organics DESCRIPTION BASE CAPACITY (MEQ) RETAINS Ion Exchangers SAX* 8% x-linked Styrene-Divinylbenzene 0.30/200mg (-) Charged Compounds SCX** 8% x-linked Styrene-Divinylbenzene 0.48/200mg (+) Charged Compounds IC Resins (see page 14-15 for complete specs on IC Resins) * Strong Anion Exchager in Acetate Form ** Strong Cation Exchanger in Hydrogen Form 4

SPE 장치 실물크기의제품도면 Extract-Clean TM and Ultra-Clean TM Columns 개선된컬럼디자인! 용매에대한저항성을강화한 Polypropylene 재질 좁은공간과 Manifolds에더욱적합하도록소형화된플랜지 Bed를더욱안정되게받쳐주는특별히고안된 Frit Extract-Clean RC Maxi-Clean Cartridges 300mg Bed For information about 96-Well SPE Plates, see page 23. 900mg Bed 600mg Bed Device Specifications DEVICE HOUSING MATERIAL FRIT MATERIAL TYPICAL PROCESSING METHOD DISTINGUISHING FEATURE Extract-Clean Column Polypropylene Polyethylene Pull with vacuum Allows large volume samples Ultra-Clean Column Treated, Inert Polypropylene Teflon Pull with vacuum Has minimal extractables Extract-Clean RC Column Polypropylene Polyethylene Pull with vacuum Compatible with robotic systems Maxi-Clean Cartridge Polypropylene Polyethylene Push with syringe Ideal for single-sample processing Prep-Sep 96-Well Plate Polypropylene Polyethylene Pull with vacuum Allows simultaneous processing of 96 samples www.dongmyung.co.kr 5

SPE 사용방법 일반적인 Solid-Phase Extraction 사용법 : 역상 (Reversed-Phase) 극성매트릭스로부터상대적으로비극성화합물을분리할때 1. 충진물의활성화 (Conditioning); 3~5ml의메탄올로튜브를씻어준다음 3-5ml의물 / 완충용액을흘려준다. ( 다음단계로진행되기전에건조되면안된다.) 2. 시료주입 (Sample Application); SPE 상단에시료를주입하여충진베드를통과시킨다. ( 가장바람직한흐름은약 1~5ml/min) 3. 튜브세척 (Tube Wash); 시료를보존하기위해 5ml의극성용매 ( 증류수, 완충용액, 또는수용성 / 유기혼합물등이가장많이사용된다.) 로씻어준다. 4. 용리 (Elution); 1~5ml/min의비극성용매로시료성분을수집튜브로용리시킨다. Method Development Kits 만약 SPE 충진물의선택이최상이라는확신이없다면, Method Development kit를사용하십시요. 이키트에는 Extract-Clean 컬럼형태의다양한충진물이포함되어있어최소의비용으로최상의충진물을선택할수있도록도와줄것이다. 수용성또는극성유기매트릭스로부터극성에서비극성화합물까지적합하게머무르게하려면역상키트를선택하고, 비극성매트릭스로부터극성화합물을머무르게하려면정상키트를선택한다. Complete Kit는역상, 정상, 그리고이온 / 양이온교환등모든용도로사용할수있다. 정상 (Normal-Phase) 비극성매트릭스로부터극성화합물을분리할때 1. 충진물의활성화 (Conditioning); 3~5ml의비극성용매로튜브를씻어준다. 2. 시료주입 (Sample Application); SPE 상단에시료를주입하여충진베드를통과시킨다. ( 가장바람직한흐름은약 1~5ml/min) 3. 튜브세척 (Tube Wash); 시료를보존하기위해 5ml의비극성용매로씻어준다. 4. 용리 (Elution); 1~5ml/min의극성용매로시료성분을수집튜브로용리시킨다. 5493 이온교환 (Ion-Exchange) 전하를가진또는잠재적인전하를가진화합물을분리할때 1. 충진물의활성화 (Conditioning); 3~5ml의증류수또는약한이온세기의완충용액 (0.01M) 으로튜브를씻어준다. 2. 시료주입 (Sample Application); SPE 상단에시료를주입하여충진베드를통과시킨다. ( 이온교환거동은역상이나정상보다느리기때문에유량은약 1ml/min로유지시킨다.) 3. 튜브세척 (Tube Wash); 5ml의증류수또는이온세기가약한완충용액으로씻어준다. 4. 용리 (Elution); 1~5ml/min의강한이온세기의완충용액 (0,1-1M) 또는수정된 ph( 시료가전하를띄지않는 ph) 로시료성분을수집, 튜브안으로용리시킨다. Method Development Kits BED WT, TUBE SIZE PART NO. Normal Phase Kit 100mg, 1.5mL 211200 (Includes 10 tubes each of Silica, Florisil, Aminopropyl, Cyano, and Diol) 500mg, 4.0mL 211300 (Includes 5 tubes each of Silica, Florisil, Aminopropyl, Cyano, and Diol) Reversed Phase Kit 100mg, 1.5mL 211001 (Includes 10 tubes each of C18, C8, C2, and Phenyl) 500mg, 4.0mL (Includes 5 tubes each of C18, C8, C2, Cyclohexyl, and Phenyl) Complete Kit 100mg, 1.5mL 210100 (Includes 10 tubes each of C18, C8, C2, Diol, Cyano, Aminopropyl, Silica, SAX, and SCX) 500mg, 4.0mL 210500 (Includes 5 tubes each of C18, C8, C2, Cyclohexyl, Diol, Cyano, Aminopropyl, Silica, SAX, and SCX) 6

C18 SPE Device C18 Specifications Functional Group: Octadecyl (C18) Base: 50μm irregularly-shaped silica (100μm High Flow), 60A porosity Endcapped: Yes Percent Carbon Loading: 6% C18, 8% High Flow C18, 17% High Capacity C18 Retention Mechanism: Strongly Non-Polar (Reversed Phase) C18 Extract-Clean EV(Extended Volumn) 컬럼 큰용량을수용할수있는방사형디자인 C18 Extract-Clean and Ultra-Clean Columns 6종의 Column 규격제공 표준 C18, 고용량 C18, 또는높은유량의 C18 중에서선택 일상적인분석에유용한 Extract-Clean 미세한추출에적합한 Ultra-Clean Column C18 Extract-Clean EV Columns C18 Extract-Clean EV Columns 100mg 10mL 30 405001 200mg 10mL 30 405150 500mg 10mL 30 405250 4887 C18 Maxi-Clean TM Cartridges 모든 Luer Tip 형태의실린지에사용가능 3 종류의 Bed 규격을제공 4882 C18 Extract-Clean and Ultra-Clean Columns C18 Extract-Clean Columns 50mg 1.5mL 100 204900 100mg 1.5mL 100 205000 200mg 4.0mL 50 205150 500mg 4.0mL 50 205250 500mg 8.0mL 30 205350 1000mg 8.0mL 30 205430 2000mg 8.0mL 30 205450 2000mg 15mL 30 205462 5000mg 25mL 20 225450 10,000mg 75mL 16 235410 High Capacity C18 Extract-Clean Columns 50mg 1.5mL 100 255050 100mg 1.5mL 100 255100 200mg 4.0mL 50 255200 500mg 4.0mL 50 255300 500mg 8.0mL 30 255350 1000mg 8.0mL 30 255430 2000mg 15mL 30 255440 5000mg 25mL 20 255450 10,000mg 75mL 16 255460 High Flow C18 Extract-Clean Columns 500mg 4.0mL 50 215250 1000mg 8.0mL 30 215430 C18 Ultra-Clean Columns 200mg 4.0mL 50 505150 500mg 8.0mL 30 505355 4886 C18 Maxi-Clean Cartridges BED WEIGHT QTY PART NO. C18 Maxi-Clean Cartridges 300mg 25 20924 300mg 50 20926 300mg 100 20928 600mg 25 20932 600mg 50 20934 600mg 100 20936 900mg 25 20940 900mg 50 20942 900mg 100 20944 C18 96-Well Prep-Sep Plates 표준형의 96-Well format C18 96-Well Prep-Sep Plates C18 Prep-Sep Plates 25mg 2.0mL 1 214996 50mg 2.0mL 1 204996 100mg 2.0mL 1 224996 4888 www.dongmyung.co.kr 7

Prevail C18 SPE Device SPE 는시료전처리의모든과정을간단명료하게해준다. Alltech 의새로운 Water Wettable C18 제품은기존 SPE 에서유기용매를이용한사전컨디셔닝과정이필요없이추출성능을쉽게향상시켜준다. 시간단축! Prevail C18은유기용매에의한사전컨디셔닝을필요로하지않기때문에 SPE의준비과정을한단계줄여준다. 용매의절감! 유기용매에의한사전컨디셔닝과정을제거하여위험한유기용매와값비싼폐용매처리비용을절감할수있다. 재검증이필요없다! 실리카지지체의 C18 흡착제는표준화되있기때문에 C18에서요구하는방법이나설정된시료처리방법으로직접대체할수있다. 일반화된응용성! 표준 C18 흡착제는유기용매에의한사전전처리과정에관계없이탁월한성능을보여준다. 따라서어떠한시료형태나응용에관계없이 C18을사용할수있다. Prevail SPE 를사용하면시료처리에필요한모든해법을찾을수있다. Ideal For: - 의약품의혈액내대사산물 - 체액중의호르몬추출시 - 천연물중의 Neutraceuticals 응용시 - 물중의살충제추출시 - 수용성매트릭스중의비극성화합물의추출시 Prevail SPE Specifications Base: Silica Functional Group: C18 Particle Size: 35-75μm Pore Size: 60A Surface Area: 500m 2 /g Carbon Load: 11% Prevail SPE Formats Extract-Clean SPE Columns Larger Bed Weights Process by Vacuum Maxi-Clean SPE Cartridges Small and Portable Process by Vacuum or by Syringe Prevail C18 Extract-Clean Columns BED WEIGHT COLUMN SIZE QTY PART NO. 100mg 1.5mL 100 605001 500mg 4.0mL 50 605250 500mg 8.0mL 30 605350 1000mg 8.0mL 30 605430 Prevail C18 Maxi-Clean Cartridges BED WEIGHT QTY PART NO. 300mg 50 605926 500mg 50 605929 900mg 50 605942 8

C18 SPE Application Hypericins from St. John s Wort Extract-Clean 고용량의 C18, 1000mg 을사용한처리과정 : A. 시료의처리 St. John s Wort 300mg 을분말로하여 5 개로나누고, 3ml 의 80/20 MeOH/H₂O 액에각각용해한후합쳐서여과하여 15ml 의여과액을만든다. 다음물로희석하여 30ml 로한다. B. 컨디셔닝 1. 5ml 의메탄올로튜브를세척한다. 2. 5ml 의증류수로튜브를세척한다. C. 시료의응용 2ml 의여과액을 SPE 의상단에넣고 C18 충진베드를서서히통과시킨다. D. 튜브세척 * 3ml 의증류수로튜브를세척한다. E. 용리 2ml 의메탄올로용리한다. * 여과액이소모될때까지시료의적하 (Load) 와세척단계를연속하여반복한다. Phenolic Acids from Echinacea Extract-Clean 고용량의 C18, 1000mg 을사용한처리과정 : A. 시료의처리 Echinacea 300mg 을분말로하여 5 개로나누고, 3ml 의메탄올에각각용해한후합쳐서여과하여 15ml 의여과액을만든다. 다음물로희석하여 30ml 로한다. B. 컨디셔닝 1. 5ml 의메탄올로튜브를세척한다. 2. 5ml 의증류수로튜브를세척한다. C. 시료의응용 2ml 의여과액을 SPE 의상단에넣고 C18 충진베드를서서히통과시킨다. D. 튜브세척 * 2ml 의증류수로튜브를세척한다. E. 용리 2ml 의메탄올로용리한다. * 여과액이소모될때까지시료의적하 (Load) 와세척단계를연속하여반복한다. 물중의염소계살충제의전처리 Extract-Clean Hi-Flow C18, 500mg 을사용한처리과정 : A. 컨디셔닝 ; 1.10ml 의 hexane 과 10ml 의 ethyl acetate 로 SPE 튜브를세척한다. 초과된용매를제거하기위해 SPE 튜브에 Air 를불어넣는다. 2. 튜브를 5ml 메탄올로세척한후 5ml 증류수로세척한다. B. 시료응용유속 20ml/min 로 100ml - 500ml (1% 메탄올을포함한 ) 의수용성시료를튜브로통과시킨다. C. 튜브세척 10ml 의증류수로세척한다음 10ml 20/80(MeOH/H₂O) 의용매로세척한다. 2 분동안튜브에 Air 를통과시켜초과된용매를제거한다. D. 용리 70/30(hexane/ethyl acetate) 2ml 로용리하고잔류물의제거를위해 2-3g 의무수황산나트륨에통과시킨다. www.dongmyung.co.kr 9

C8, C2 SPE Device C8 Specifications Functional Group: Base: Endcapped: Percent Carbon Loading: Retention Mechanism: Octyl (C8) 50μm irregularly-shaped silica, 60A porosity Yes 4.5% C8, 8.5% High Capacity C8 Moderately Non-Polar (Reversed Phase) C2 Specifications Functional Group: Ethyl (C2) Base: 50μm irregularly-shaped silica, 60A porosity Endcapped: Yes Percent Carbon Loading: 5.5% Retention Mechanism: Weakly Non-Polar (Reversed Phase) C8 Extract-Clean Columns 3종의컬럼규격제공 표준 C8과고용량의 C8 사이의충진물선택가능 C2 Extract-Clean Columns 3 종의컬럼규격의제품을공급 4885 4885 C8 Extract-Clean Columns C8 Extract-Clean Columns 50mg 1.5mL 100 205900 100mg 1.5mL 100 206000 200mg 4.0mL 50 206150 500mg 4.0mL 50 206250 500mg 8.0mL 30 206350 C2 Extract-Clean Columns C2 Extract-Clean Columns 50mg 1.5mL 100 207362 100mg 1.5mL 100 207300 200mg 4.0mL 50 207450 500mg 4.0mL 50 207550 10,000mg 75mL 16 207616 High Capacity C8 Extract-Clean Columns 50mg 1.5mL 100 206450 100mg 1.5mL 100 206500 200mg 4.0mL 50 206550 500mg 4.0mL 50 206600 500mg 8.0mL 30 206650 C8 Maxi-Clean Cartridges 모든 Luer Tip 형태의실린지사용가능 3종의충진 Bed 공급 C2 Maxi-Clean Cartridges 모든 Luer Tip 형태의실린지사용가능 4882 C8 Maxi-Clean Cartridges BED WEIGHT QTY PART NO. C8 Maxi-Clean Cartridges 300mg 25 20948 300mg 50 20950 300mg 100 20952 600mg 25 20956 600mg 50 20958 600mg 100 20960 900mg 25 20964 900mg 50 20966 900mg 100 20968 C2 Maxi-Clean Cartridges BED WEIGHT QTY PART NO. C2 Maxi-Clean Cartridges 300mg 25 210062 300mg 50 210064 300mg 100 210066 4880 10

Silica SPE Device Silica Specifications Functional Group: None Base: 50μm irregularly-shaped silica, 60A porosity Percent Carbon Loading: 0% Retention Mechanism: Strongly Polar (Normal Phase) Silica Extract-Clean EV 컬럼 방사형상으로큰용량의응용에적합 EPA Method 3630C에유용 Silica Extract-Clean and Ultra-Clean 컬럼 6종의컬럼규격의제품을공급 일상적인분석에적합한 Extract-Clean 미세한추출에적합한 Ultra-Clean EPA Method 3630C에유용 Silica Extract-Clean EV Columns BED WEIGHT TUBE SIZE EPA METHOD QTY PART NO. Silica Extract-Clean TM EV Columns 100mg 10mL 30 409001 200mg 10mL 30 409150 500mg 10mL 3630C 30 409250 4887 Silica Maxi-Clean Cartridges 모든 Luer Tip 실린지에사용가능 3종의충진베드를공급 4886 4882 Silica Extract-Clean and Ultra-Clean Columns BED WEIGHT TUBE SIZE EPA METHOD QTY PART NO. Silica Extract-Clean Columns 50mg 1.5mL 100 209062 100mg 1.5mL 100 209000 200mg 4.0mL 50 209150 500mg 4.0mL 50 209250 500mg 8.0mL 3630C 30 209200 1000mg 8.0mL 3630C 30 209100 2000mg 8.0mL 30 209202 2000mg 15mL 3630C 30 209362 5000mg 25mL 20 22935 10,000mg 25mL 20 239300 10,000mg 75mL 16 239310 20,000mg 75mL 16 239322 30,000mg 75mL 16 239330 Silica Ultra-Clean Columns 200mg 4.0mL 50 509150 500mg 8.0mL 3630C 30 509225 Silica Maxi-Clean Cartridges BED WEIGHT QTY PART NO. Silica Maxi-Clean Cartridges 300mg 25 20972 300mg 50 20974 300mg 100 20976 600mg 25 20980 600mg 50 20982 600mg 100 20984 900mg 25 20988 900mg 50 20990 900mg 100 20992 Silica 96-Well Prep-Sep Plates 표준형 96-Well Plate 4888 Silica 96-Well Prep-Sep Plates Silica Prep-Sep Plates 25mg 2.0mL 1 210996 50mg 2.0mL 1 209096 100mg 2.0mL 1 229096 www.dongmyung.co.kr 11

Aminopropyl(NH₂) SPE Device NH₂Specifications Functional Group: Aminopropyl (NH₂) Base: 50μm irregularly-shaped silica, 60A porosity Endcapped: No Percent Carbon Loading: 5% Retention Mechanism: Weak anion exchange with aqueous matrix, polar with non-polar organic matrix. (Normal Phase) NH₂Extract-Clean EV 컬럼 방사형상으로큰용량의응용에적합 NH₂Extract-Clean and Ultra-Clean Columns 3 종의컬럼규격을제공 일상적인분석에적합한 Extract-Clean 미세한추출에적합한 Ultra-Clean NH₂Extract-Clean EV Columns NH₂Extract-Clean EV Columns 500mg 10mL 30 407250 4887 NH₂Maxi-Clean Cartridges 4885 모든 Luer Tip 실린지에사용가능 NH₂Extract-Clean and Ultra-Clean Columns NH₂Extract-Clean Columns 50mg 1.5mL 100 211062 100mg 1.5mL 100 211000 200mg 4.0mL 50 211025 500mg 4.0mL 50 211150 1000mg 8.0mL 30 211153 NH₂Ultra-Clean Columns 200mg 4.0mL 50 511025 500mg 4.0mL 50 511150 NH₂Maxi-Clean Cartridges BED WEIGHT QTY PART NO. NH₂Maxi-Clean Cartridges 300mg 25 210042 300mg 50 210044 300mg 100 210046 4880 12

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Alumina SPE Device Alumina Specifications Sorbent: Base: ph: Particle Size: Retention Mechanism: Alumina Aluminum Oxide, 100A porosity 4.5 (Acidic), 10.0 (Basic), 7.5 (Neutral) 130μm Lewis acid/base, polar, and ion exchange (Normal Phase) Alumina Extract-Clean EV 컬럼 방사형상으로큰용량의응용에적합 Alumina Extract-Clean Columns 4887 산, 염기. 및중성시료의응용에유용 4종의컬럼규격의제품을공급 Alumina Extract-Clean EV Columns ALUMINA TYPE Alumina Extract-Clean EV Columns Acidic 500mg 10mL 30 410450 Basic 500mg 10mL 30 410250 Neutral 500mg 10mL 30 410850 Neutral 500mg 10mL 1000 410710 Alumina Maxi-Clean Cartridges 4886 산, 염기및중성시료의응용에유용 모든 Luer Tip 실린지에사용가능 Alumina Extract-Clean Columns ALUMINA TYPE Alumina Extract-Clean Columns Acidic 100mg 1.5mL 100 228200 Acidic 500mg 4.0mL 50 228350 Basic 100mg 1.5mL 100 228000 Basic 500mg 4.0mL 50 228150 Neutral 100mg 1.5mL 100 228400 Neutral 500mg 4.0mL 50 228550 Neutral 2000mg 15mL 30 22856 Neutral 10,000mg 75mL 16 228580 4881 Alumina Maxi-Clean Cartridges ALUMINA TYPE BED WEIGHT QTY PART NO. Alumina Maxi-Clean Cartridges Acidic 300mg 25 210094 Acidic 1800mg 25 210097 Basic 300mg 25 210093 Basic 1800mg 25 210096 Neutral 300mg 25 210095 Neutral 1800mg 25 210098

Other SPE Device Alltech은여러분이기본적으로예상하는것이상의고품질의광범위한 SPE 충진제를제공한다. 제품별규격은앞표지안쪽표에수록되어있다. 만약여기서필요로하는정보를찾지못하였다면 Alltech에연락하십시요. 그러면 Alltech은여러분이원하는자료를제공할수있을것입니다. Diol SPE Devices 정상 (Normal Phase) 60A Pore Size 4880 4884 Cyclohexyl Extract-Clean Columns 역상 (Reversed Phase) 60A Pore Size 5095 Diol SPE Devices Diol Extract-Clean Columns 50mg 1.5mL 100 208062 100mg 1.5mL 100 208000 200mg 4.0mL 50 208150 500mg 4.0mL 50 208250 Diol Extract-Clean EV Column 500mg 10.0mL 30 407650 Cyclohexyl SPE Devices Cyclohexyl (CH) Extract-Clean Columns 200mg 4.0mL 50 207150 500mg 4.0mL 50 207250 Diol Maxi-Clean Cartridges 300mg 25 210082 300mg 50 210084 300mg 100 210086 DVB Extract-Clean Columns Cyanopropyl (CN) SPE Devices 정상 (Normal Phase) 60A Pore Size C18 보다용량이큰시료에적합 초순수 Divinylbenzene의사용으로팽창에의한손상감소 4885 4887 4881 Cyanopropyl SPE Devices Cyanopropyl (CN) Extract-Clean Columns 50mg 1.5mL 100 209462 100mg 1.5mL 100 209300 200mg 4.0mL 50 209450 500mg 4.0mL 50 209550 500mg 8.0mL 30 209650 Cyanopropyl (CN) Maxi-Clean Cartridges 300mg 25 210032 300mg 50 210034 300mg 100 210036 DVB SPE Devices DVB Extract-Clean Columns 25mg 1.5mL 100 220025 50mg 1.5mL 100 220050 100mg 1.5mL 100 220100 500mg 4.0mL 50 220500 4884 www.dongmyung.co.kr

Other SPE Device and SuperClean TM SPE Columns Phenyl SPE Devices 역상 (Reversed Phase) 60A Pore Size Phenyl SPE Devices Phenyl Extract-Clean Columns 50mg 1.5mL 100 232062 100mg 1.5mL 100 232000 200mg 4.0mL 50 232150 500mg 4.0mL 50 232300 Phenyl Maxi-Clean Cartridges 300mg 25 22002 300mg 50 22003 300mg 100 22004 4880 4885 Alltech's Exclusive SuperClean Columns 고형물질과용해되지않는오염물제거시용이 1~2L의수용성이동상을정화 신속한처리를위해 In-Line 여과장치와함께사용가능 Polypropylene 재질의 75mL 하우징채택 Alltech의독점적인 Super Clean SPE 컬럼은이동상의정화를위해특별하게 5950 설계되었다. SuperClean SPE 컬럼은독특하게활성탄소와 Pre-Filter로혼합되어있어유기오염물질과고형입자를동시에제거할수있다. SuperClean Column은 HPLC 컬럼의수명과바탕선을안정시키고분석결과를획기적으로향상시켜준다. 가장효율적인방법으로다양한이동상의용량을처리하기위해서는 SuperClean 컬럼을 In-Line Filtration System과함께사용하는것이다. Carbograph Extract-Clean and Ultra-Clean Columns 수용성매트릭스에서극성유기물을 Graphitized Carbon에포집시사용 농약살충제와제초제로부터산, 염, 중성물질추출시사용 4891 Carbograph SPE Devices Carbograph Extract-Clean Columns 150mg 4.0mL 50 210142 300mg 8.0mL 30 210101 500mg 8.0mL 30 210150 1000mg 15mL 20 210121 Carbograph Ultra-Clean Columns 150mg 4.0mL 50 510142 300mg 8.0mL 30 510101 Drying Devices 무수황산나트륨으로충진되어있어 추출액중수분제거에유용 Drying Devices 4895 SuperClean SPE Columns BED WEIGHT QTY PART NO. SuperClean SPE Columns,75mL 16 29901 BED WEIGHT QTY PART NO. Drying Devices Maxi-Clean 2g Anhydrous Sodium Sulfate 100 219001 Extract-Clean 3g Anhydrous Sodium Sulfate 100 219002 For information on using the In-Line Filtration System, see page 25.

SAX and SCX SPE Device SAX Specifications SCX Specifications Functional Group: Base: Capacity: Retention Mechanism: Tetramethyl Ammonium Styrene-divinylbenzene 0.15meq/100mg Strong anion exchanger in acetate form; retains (-) charged compounds. Functional Group: Base: Capacity: Retention Mechanism: Benzenesulfonic Acid Styrene-divinylbenzene 0.24meq/100mg Strong cation exchange in hydrogen form; retains (+) charged compounds. SAX Extract-Clean Columns 2 종의컬럼규격을제공 SCX Extract-Clean Columns 3 종의컬럼규격을제공 4889 4889 SAX Extract-Clean Columns SAX Extract-Clean Columns 50mg 1.5mL 100 209662 100mg 1.5mL 100 209600 200mg 4.0mL 50 209625 500mg 4.0mL 50 209750 SAX Extract-Clean Columns SAX Extract-Clean Columns 50mg 1.5mL 100 209862 100mg 1.5mL 100 209800 200mg 4.0mL 50 209825 500mg 4.0mL 50 209950 1000mg 8.0mL 50 209930 SAX Extract-Clean EV Columns 방사형상으로큰용량의응용에적합 SCX Extract-Clean EV Columns 방사형상으로큰용량의응용에적합 5569 5569 SAX Extract-Clean EV Columns SAX Extract-Clean EV Column 500mg 10mL 30 409750 SAX Extract-Clean EV Columns SAX Extract-Clean EV Column 500mg 10mL 30 409950 SAX Maxi-Clean Cartridges SCX Maxi-Clean Cartridges 모든 Luer Tip 실린지에사용가능 모든 Luer Tip 실린지에사용가능 4897 4897 SAX Maxi-Clean Cartridges SAX Maxi-Clean Cartridges BED WEIGHT QTY PART NO. SAX Maxi-Clean Cartridges 600mg 25 21906 600mg 50 21907 600mg 100 21908 BED WEIGHT QTY PART NO. SAX Maxi-Clean Cartridges 600mg 25 21901 600mg 50 21902 600mg 100 21903 SAX 96-Well Prep-Sep Plates 표준형 96-Well Plate SCX 96-Well Prep-Sep Plates 표준형 96-Well Plate 4888 4888 SAX 96-Well Plates SAX Prep-Sep Plates 25mg 2.0mL 1 219696 50mg 2.0mL 1 209696 100mg 2.0mL 1 229696 SAX 96-Well Plates SAX Prep-Sep Plates 25mg 2.0mL 1 219896 50mg 2.0mL 1 209896 100mg 2.0mL 1 229896 www.dongmyung.co.kr

Ion Chromatography Resin SPE Device Maxi-Clean and Extract-Clean IC Devices 이온분석전에매트릭스의간섭물질제거 7 가지화학적성질에의한다양한형태의특정문제를해결 Maxi-Clean 또는 Extract-Clean 형태로제작공급 Alltech IC 화학의목표는다양한시료처리문제를해결하는데있다. IC-RP 는계면활성제, 유기산, 단백질그리고다른유기계면활성 제를제거하는데확실한방법을제공한다. 많은이온크로마토그래피응용에서가장까다로운부분은시료매트릭스로부터간섭하는성분을제거하는것이다. 이러한간섭물질은함께용리되거나또는중요한피크를간섭하며, 컬럼에과부하를주고, 컬럼충진물에결합되어세척할수없게하므로컬럼수명을급격히단축시킨다. Alltech의독특한 IC SPE 장치는 1) 2) 이러한많은간섭물질을제거한다. 각각의 polypropylene 장치는 0.5ml 또는 1.5ml의정제된 Polystyrene Resin과 20μm의 Polyethylene 프릿을포함한다. Resin은시료의특별한유형의성분을머무르도록작용한다. Maxi-Clean cartridge는모든 luer 형태의실린지와함께사용할수있고, Extract Clean 컬럼은전형적인 Vacuum manifold 용이다. IC-H, IC-OH는시료를주입하기전 ph를조절한다. Nitrite와 Phosphate 같은약음이온의회수율은 IC-H 전처리후다소떨어지는일이있다. IC-Ag, IC-Ba는각각할로겐화물과황산염의반응침전물을제거하기위해선택적으로사용한다. 만약할로겐화물과황산염의농도가높다면전처리하는동안저농도의다른음이온은손실을볼수도있다. IC-Na는시료의 ph 변화없이시료로부터양이온을제거한다. IC-Chelate는시료중의전이금속과다가의양이온을제거하는데사용한다. 1) 2) R. Saari-Nordhaus, J.M. Anderson, Jr., and I.K. Henderson, Am. Lab. August (1990) 19. I.K Henderson, R. Saari-Nordhaus, and J.M. Anderson, Jr., J. Chromatogr. 546 (1991) 61. Mixed-Mode RP-OH 는염기성시료에서 ph 를떨어뜨리면서 양이온과소수성성분을동시에제거하는데사용한다. Maxi-Clean and Extract-Clean IC Devices CARTRIDGE PACKING RETAINS CAPACITY (MEQ) 0.5ML/1.5ML 응용 IC-RP Polystyrene 소수성 Variable 계면활성제, 유기산, 다른유기물질, 무기이온은통과 IC-OH -OH - 의강음이온교환체음이온 0.6 / 1.5 수산기 (OH - ) 에의한음이온교환체, 산성시료의 ph 를 증가시켜시료의음이온을농축하거나제거시사용 IC-H -H + 의강양이온교환체양이온 0.8 / 2.0 H + 기에의한양이온교환체. 염기성시료의 ph 를 떨어뜨려시료의양이온을농축하거나제거시사용 IC-Ag -Ag + 의강양이온교환체 Chloride 0.8 / 2.0 Ag- 할로겐염의조성물를통과시켜과잉할로겐화물을 Iodide Bromide 제거시사용 IC-Ba -Ba +2 의강양이온교환체 Sulfate 0.8 / 2.0 BaSO₄ 의조성물을통과시켜과잉의황산염을제거시사용 IC-Na -Na + 의강양이온교환체양이온 0.8 / 2.0 Na + 에의한양이온교환. 시료의 ph 변화없이시료중의 양이온을농축하거나제거시사용 IC-Chelate 킬레이트기를포함하는다가의 0.2 / 0.5 Na + 에의한다가의양이온과전이금속교환. Styrene-DVB 금속이온시료의다가의양이온이나전이금속을농축하거나제거시사용 (Iminodiacetate 에 Na + ) IC Mixed-Mode Polystyrene/-OH - 기가소수성 Variable-0.6 산성시료에서 ph 를증가시키면서음이온과소수성성분을 RP-OH 있는강음이온교환체성분과동시에제거시사용 음이온 IC Mixed-Mode Polystyrene/-H + 기가소수성 Variable-0.8 염기성시료에서 ph 를떨어뜨리면서양이온과소수성성분을 RP-H 있는강양이온교환체성분과동시에제거시사용 양이온

Ion Chromatography Resin SPE Device IC Extract-Clean Columns Vacuum Manifold와함께사용가능 2종의베드용량제품을공급 4894 Procedure 1. Methylene Chloride와 1% NaOH( 각각 5ml) 혼합용매로 1g의 Aniline을추출 2. 1단계카트리지에서수층 4ml를사용한다. 3. 최초로용리된 1ml는버리고, 4. 나머지용리액을수집하여분석한다. IC Extract-Clean Columns RESIN TYPE TUBE SIZE QTY PART NO. 0.5mL Bed Volume IC-RP 4.0mL 50 40260 IC-OH 4.0mL 50 40262 IC-H 4.0mL 50 40264 IC-Ag 4.0mL 50 105050 IC-Ba 4.0mL 50 40268 IC-Na 4.0mL 50 40270 IC-Chelate 4.0mL 50 40250 1.5mL Bed Volume IC-RP 4.0mL 30 140252 IC-OH 4.0mL 30 140254 IC-H 4.0mL 30 140256 IC-Ag 4.0mL 30 140258 IC-Ba 4.0mL 30 140261 IC-Na 4.0mL 30 140263 IC-Chelate 4.0mL 30 140265 IC SPE Cartridges 적은양의시료처리시사용 2종의베드용량제품을공급 4893 IC Extract-Clean Columns RESIN TYPE QTY PART NO. 0.5mL Bed Volume IC-RP 50 30260 IC-OH 50 30262 IC-H 50 30264 IC-Ag 50 30266 IC-Ba 50 30268 IC-Na 50 30270 IC-Chelate 50 30250 IC Mixed-Mode RP-OH 25 30259 IC Mixed-Mode RP-H 25 30267 1.5mL Bed Volume IC-RP 25 30252 IC-OH 25 30254 IC-H 25 30256 IC-Ag 25 30258 IC-Ba 25 30261 IC-Na 25 30263 IC-Chelate 25 30265 www.dongmyung.co.kr

Vacuum Manifolds and Accessories K A Vacuum Manifold Components A. Glass Chamber B. Vacuum Valve and Gauge C. Polypropylene Lid D. Stopcock Valves A F E C H B E J E. Collection Rack Plates F. Support Posts for Collection Racks G.Retaining Clips for Collection Racks H. Lid Legs I. Manifold Inlet Caps J. Polypropylene Needles K. SPE Reservoirs I G D (sold separately) 4872 Alltech Vacuum Manifolds 16-Port Manifold 12-, 16-, and 24-Port Manifolds 관찰이용이한 Glass Chamber 모든표준형 Male Luer 장치사용가능 16 개의시료까지처리가능 Vacuum manifold는동시에여러시료를처리할수있어시간과노동력을절감하고, Manifold 시스템은아래구성품목과같이완벽한시스템으로공급된다. Stainless steel 또는 Teflon needle은별도로공급가능하다. Alltech Manifolds Include*: Glass Chamber, Vacuum Valve and Gauge, Polypropylene Lid, Stopcock Valves, Collection Racks, Support Posts, Retaining Clips, Manifold Inlet Caps, Lid Legs, and Polypropylene Needles. * The 12- Port Manifold는폐수용기가포함된다. 12-Port Manifold 12개의시료까지처리가능 한개의폐수용기포함 12-Port Manifold & Accessories 12-Port Vacuum Manifold Ea 210351 Replacement Parts for 12 Port Manifolds Lid, Gaskets, and 12 Stopcocks Ea 212001 Glass Chamber Ea 213212 Vacuum Gauge, Valve, & Glass Chamber Ea 212304 Collection Rack, 12-Port Size* Ea 212518 Gaskets, 12-Port Size 2 212112 One-Way Stopcocks 12 213112 Waste Container 2 210033 * 12-Port Collection Rack 은 3 개의지지대, 밑판, 13- 과 16mm plate, 자동시료주입기용 Vial plate, volumetric plated 와 retaining clip 12 개가포함된다. 4869 16-Port Manifold & Accessories 16-Port Vacuum Manifold Ea 210016 Replacement Parts for 16 Port Manifolds Lid, Gaskets, and 16 Stopcocks Ea 210216 Glass Chamber Ea 210116 Vacuum Gauge, Valve, & Glass Chamber Ea 210316 Collection Rack, 16-Port Size** Ea 210416 Gaskets, 16- and 24-Port Size 2 210724 One-Way Stopcocks 16 211521 24-Port Manifold 24 개의시료까지처리가능 24-Port Manifold & Accessories 24-Port Vacuum Manifold Ea 210224 Replacement Parts for 24 Port Manifolds Lid, Gaskets, and 24 Stopcocks Ea 211224 Glass Chamber Ea 210124 Vacuum Gauge, Valve, & Glass Chamber Ea 210324 Collection Rack, 24-Port Size** Ea 210424 Gaskets, 16- and 24-Port Size 2 210724 One-Way Stopcocks 24 211524 **16- and 24-Port Collection Rack 은 3 개의지지대, 밑판, 보조개 Plate, 13- and 16mm plates, and 12 retaining clip 이포함된다. 4870 4871

Vacuum Manifold Accessories 모든규격의 Manifold 에적합한소모품공급 Drying Attachments Manifold는모든필요한표준부품과소모품이완벽하게구성되어공급되며, 대체소모품은별도판매가가능하다. 이특수한건조판은수집튜브에직접가스를흘려주어용출물을건조시키는데사용한다. 5951 4878 Replacement Parts for All Size Manifolds Vacuum Gauge and Valve Ea 212203 Retaining Clips For Collections Racks 12 212912 Female Luer Inlet, Nylon 2 212002 Male Luer Outlet, Nylon 2 212120 Caps for Inlets 50 211234 Lid Legs 4 410510 Collection Rack Posts 3 410410 Drying Attachments 12-Port Drying Attachment 1 212100 16-Port Drying Attachment 1 212117 24-Port Drying Attachment 1 212124 Manifold Needles Tygon Tubing Teflon needle은 1회용으로많은다른 Manifold 모델에도사용가능하며, 수집튜브안까지연장되어있어교차오염되는것을제거한다. 더욱이시료의추출물을실제로제거하기위해서완전한테프론유로로제공된다. 밸브와함께하거나또는일반적인사용이가능하다. 스테인레스스틸과 Polypropylene 바늘이제공된다 Tygon 진공튜빙은내부벽이고밀도로되어있고유연하며, 표준상태 (24 ) 에서 29.9 Hg진공에도안전하다. 독성이없고, 무해하며, 투명하다. Stainless Steel Teflon Polypropylene Valved Teflon 4874 3849 Manifold Needles Teflon Needles 100 412410 Teflon Needles 500 412450 Valved Teflon Needles 25 411525 Valved Teflon Needles 50 411550 Stainless Steel Needles 12 212400 Stainless Steel Needles 16 210816 Stainless Steel Needles 24 210824 Polypropylene Needles 12 212412 Polypropylene Needles 16 210916 Polypropylene Needles 24 210924 Tygon Vacuum Tubing 1/8" ID, 3/8" OD 50 ft 6465 3/16" ID, 9/16" OD 10 ft 6467 1/4" ID, 3/4" OD 10 ft 6469 3/8" ID, 7/8" OD* 10 ft 6472 * 진공게이지와밸브용으로사용한다. www.dongmyung.co.kr

SPE Bulk Packings and Tube Components Extract-Clean Reservoirs and Frits Extract-Clean Caps and Adaptors 4922 5952 Extract-Clean Reservoir는 Polypropylene 재질의공컬럼과 Male luer 배출구로되어있다. 프릿은 20μm Polyethylene 재질로되어있다. 빈저장용기 (reservoir) 와프릿을이용하여사용자스스로 SPE 튜브를만들수있다. 여과기용컬럼에서처럼 20μm까지의작은입자를잘제거하기위해서빈 Reservoir 바닥에 2개의프릿을설치한다. Extract-Clean 컬럼의상단에는마개를사용할수있다. 출구캡은모든 Male Luer tip 에적합하다. Extract-Clean 의상단은실린지아답터에알맞게되어있으며, Robot 호환컬럼을제외하면, Extract-Clean 상단의모든 Male Luer 장치에부착할수있어실린지로 Extract-Clean 컬럼을처리할수있게하거나빈 Reservoir를더하여시료의용량을증가시킬수있다. Bulk Reservoirs and Frits Extract-Clean Empty Reservoirs 1.5mL 100 210001 1.5mL 500 210011 4.0mL 100 210104 8.0mL 100 210208 15.0mL 100 210315 25.0mL 100 210425 75.0mL 50 210575 Extract-Clean EV Empty Reservoirs Up to 10mL Sample Volume 100 430050 Up to 10mL Sample Volume 1000 420011 Extract-Clean Loose Frits For 1.5mL Reservoir 100 211401 For 4.0mL or RC Reservoir 100 211404 For 8.0mL Reservoir 100 211408 For 15.0mL Reservoir 100 211412 For 25.0mL Reservoir 100 211416 For 75.0mL Reservoir 100 211775 Extract-Clean Filter Tubes 1.5mL Reservoir with 2 Bottom Frits Installed 100 211101 4.0mL Reservoir with 2 Bottom Frits Installed 50 211104 8.0mL Reservoir with 2 Bottom Frits Installed 50 211108 75.0mL Reservoir with 2 Bottom Frits Installed 50 210775 Maxi-Clean Housings and Frits Housings Small Maxi-Clean Body, for up to 300mg 100 20131 Medium Maxi-Clean Body, for up to 600mg 100 20157 Large Maxi-Clean Body, for up to 900mg 100 20159 Caps for All Body Sizes 100 20168 Frits Frits for All Body Sizes* 100 211404 Bottom Frits for Large Body 100 211406 * Note: The Large Body requires a different bottom frit. Extract-Clean Caps and Adaptors Inlet Caps for 1.5mL 50 220000 Inlet Caps for 4.0mL 50 220301 Inlet Caps for 8.0mL 50 220600 Inlet Caps for 15.0mL 50 221200 Inlet Caps for 25.0mL 50 221006 Inlet Caps for 75.0mL 50 227503 Outlet Caps for Male Luers 50 220710 Syringe Adaptors for 1.5mL, 4.0mL, 15 210705 and 8.0mL Reservoirs Syringe Adaptors for 15mL and 25mL Reservoirs 5 210707 Syringe Adaptors for 75mL Reservoirs 5 210709 Bulk Solid-Phase Extraction Packings Alltech에서공급하는큰포장의충진제는 Alltech의 Extract-Clean, Maxi-Clean 과기타 SPE 제품과동일한충진제이다. Bulk SPE Packings DESCRIPTION MESH QTY PART NO. Non-Polar Adsorbents C18 (6%) 100g 211502 C18 (17%) 100g 211503 C8 100g 211504 C2 100g 211506 Phenyl 100g 211522 Polar Adsorbents Silica 100g 211512 NH₂ 100g 211516 Diol 100g 211518 Ion Exchange SCX (Strong Cation Exchange) 100g 211520 SAX (Strong Anion Exchange) 100g 211510 Carbograph 1 120/400 25g 1769 4900

96-Well Sample Prep Plates 96-Well Plates 는시료준비속도를높여준다! Prep-Sep 96-Well SPE Plates 96개의시료를동시에준비 가장최신의자동화시스템에적합한호환성 Well 당 2ml의시료용량까지이용가능 다양한 SPE 재료를이용가능 용매에대한저항성과내성이강한 Polypropylene 재질사용 모든 96-Well 진공 Manifold에적합 Alltech의 96-Well Plate는표준형 96-Well microtiter plate 형상에기초를두고있다. 96개의시료를동시에처리하므로수작업에따른오차와조작자의지루함을줄일수있다. 이러한 Plate에다양한 Robotic 시스템들과완전하게호환되어사용될수있다. 본래시료의위치를표시한후 96-Well Plate로직접시료를추출하면시료의이동간손실과교차오염원을제거할수있어 GLP 에서요구하는재현성, 추적성, 정확성을제공한다. Prep-Sep 96-Well Packed Plates Prep-Sep 96-Well Packed Plates PACKING BED WEIGHT WELL SIZE QTY PART NO. Reversed Phase C18 25mg 2.0ml 1 214996 C18 50mg 2.0ml 1 204996 C18 100mg 2.0ml 1 224996 Normal Phase Silica 25mg 2.0mL 1 219096 Silica 50mg 2.0mL 1 209096 Silica 100mg 2.0mL 1 229096 Ion Exchangers SAX 25mg 2.0mL 1 219696 SAX 50mg 2.0mL 1 209696 SAX 100mg 2.0mL 1 229696 SCX 25mg 2.0mL 1 219896 SCX 50mg 2.0mL 1 209896 SCX 100mg 2.0mL 1 229896 96-Well Collection Plates and Cap Mats Close-up of underside of SPE Plate showing packed-bed and well tip. DESCRIPTION WELL VOLUME QTY PART NO. Polypropylene, Round Well 1.0mL 25 90284 Polypropylene, Square Well 2.0mL 10 80902 Polyethylene Cap Mat for Round Wells 30 90286 Polyethylene Cap Mat for Square Wells 10 80905 SPE plates are available with a variety of packing materials. Extract using a vacuum manifold directly into a collection plate. 4898 UNI-FILTER 96-Well Filtration Plates UNI-FILTER 96-Well Filtration Plates 화학적, 열적저항성을위해유리로충진된 Polypropylene 하우징 0.45μm 세공크기의멤브레인공급가능 모든 96-Well 진공 Manifold에적합 2mL 정방형 Wells UNI-FILTER 96-Well Plate UNI-FILTER 96-Well Plate 0.45um Nylon, 2mL 25 6926 0.45um Cellulose Acetate, 2mL 25 6928 0.45um PVDF (hydrophilic*), 2mL 25 6930 0.45um PVDF (hydrophobic), 2mL 25 6932 UNI-FILTER plates for 96-Well filtration. * 만약시료가수용성이면친수성 PVDF 를선택하고, 비수용성이거나낮은추출율을요구할때는소수성 PVDF 를선택하면된다. 5955 www.dongmyung.co.kr

Vacuum Stations Benchtop Vacuum Station 29.5" Hg의진공도 휴대용의낮은유지보수 진공수준이연속적으로 LCD상에표시 Vacuum/Pressure Station 20" Hg의진공도와 18 psig의압력 긴수명의 Diaphragm 탁월한경제성 Alltech의 Benchtop Vacuum Station은다재다능하고강력하며, 사용자친화적인대부분의진공을이용한응용에적합한시스템이다. 서로다른응용을위해유연한 3가지조작모드가제공된다. Continuous Vacuum Mode 는 1~120분시한으로진공을최대한빨아들인다. Continuous Vent Mode 는펌프헤드의증기를제거한다. Set Point Mode 는진공도를선택하게하고절대진공센서에의해진공도를유지하게한다. 즉, 고도나대기압에관계없이진공을재생하여실행한다. 따라서여과나고상추출작업에완벽한펌프성능을제공한다. Benchtop Vacuum Station Specifications 4178 Vacuum/Pressure station은바쁜실험실에서요구되는경제적인선택이다. 간단한스위치조작으로진공에서압력상태로변환될수있다. 이러한장치는하나의 Single-head pump, 진공 / 압력게이지, 그리고 SS 조절밸브로구성되어있다. Free air capacity = 0.5cu.ft/min. Vacuum/Pressure Station Specifications 5691 Maximum Pull: 725mm Hg (29.5") Flowrate: 6L/min Timer: 1~120 minutes Dimensions: 8.7" W x 9.5" D x 4.5" H (22cm W x 24.2cm D x 11.5cm H) Vacuum: Pressure: Free Air Capacity: Dimensions: 20" Hg 18psig 0.5cu. ft/min 8" W x 7.5" D x 8.75" H (20.32cm W x 19.05cm D x 22.23cm H) Benchtop Vacuum Station Vacuum/Pressure Station DESCRIPTION PART NO. 115V 6417 230V 6419 DESCRIPTION PART NO. 115V 1670 230V 1595 Vacuum/Pressure Replacement Parts/Accessories DESCRIPTION PART NO. Replacement Parts Vacuum/Pressure Gauge Connector Kit 1590 Includes Vacuum Regulation Valve Assembly, Pressure Regulation Valve Assembly, and a Manifold Chamber Service Kit 1592 (Includes Plastic Top with Retainers, Valves, Nitrile Diaphragm and Eccentric Assembly Accessories Vacuum/Pressure Gauge 1597 4384

Vacuum Filtration Systems High-Volume Filtration 은연결시누수가없고, 유량이낮아지지않으며, 유리와같이깨질우려가없어야한다! Filter Funnel In-Line Filter Cap 상호교환할수있는 Funnel 과 In-Line Systems 이동단계가필요없이 GL-45 Reservoir 로직접여과 깨지지않으며, 용매저항성이큰불활성의 Polypropylene 재질 안정하고누수의우려가없는나사선연결 여과시유속을증가시키는망상조직의여과지지체 상호교환할수있는 Funnel 과어떠한여과용량도다룰수있는 In-Line Filter Cap Alltech 의새로운진공여과제품은여과공정을단순화시키고여과량을증가시켜주며여과 Funnel 은경비와시간을절약시켜준다. 그리고불활성의견고한폴리머재질로제작되었기때문에여과시유리제품에서와같이깨지는소리를듣지않게될것이다. Vacuum Filter Funnel Base 4391 In-Line Filter Unit Base 4385 Vacuum Filter Funnels For 100mL~2L Filtration Tasks Alltech 의진공여과 Funnel 은불활성이고, 견고하며, 유리나일회용제품을선택하는것보다경제적이다. 내구성이좋고, 누수를방지할수있는나사선연결로모든 47mm 직경의멤브레인을사용할수있다. 여과기의나사선은모든 GL-45 나사선을가진병과직접연결할수있어성가신클램프를제거해주고, 가지달린플라스크또는별도의운반단계를없애준다. 각각의 Unit 를포함하고있는 6 개의진공튜빙어댑터중하나를사용하여여과기기저를진공원에직접연결하면된다. 높은용매저항성, 용매에의한추출률이낮은 poly propylene 구조물은이동상의여과와탈기그리고배양액여과에이상적이다. 1L 와 2L funnel 용량을선택할수있다. In-Line Vacuum Filter For Multi-Liter Filtration Tasks 만약고용량의여과작업을한다면, 또는시스템이여과액의대기중노출을막을필요가있다면 Alltech 의 In-Line 여과시스템을사용하면된다. In-Line 시스템은안정하고누수가없는기저를사용하지만, Funnel 을 In-line 여과캡으로대체하면 In-line 여과캡에연결된튜빙은모든용기에서시스템으로액체를직접끌어당긴다. 액체는원래의용기에서 Funnel 로쏟아지는경우가없어서대기중에노출되거나위험물질과접촉되는일이줄어든다. In-line 여과캡의용매저항성, poly propylene 유로의낮은추출률은모든표준형 47mm 멤브레인여과기를수용한다. 이것은오직 In-Line 여과기만이멤브레인여과기위에공기가축적될때흐름을쉽게복원시켜주는시스템이다. 문제가발생했을때장치를분해하고멤브레인을교체해야하는타시스템과는달리, Alltech 만이독점공급하는내장형 Priming 밸브는멤브레인을교환하거나유로를변경할필요없이짧은시간에흐름을복원시켜준다. Using the In-Line System for SPE In-Line Filtration System 은간단한조작만으로고용량의 SPE 를응용할수있다. 1/4-28 나사산구멍을노출시키면서 Inlet Port 로부터튜빙을제거한다. 1/4-28 의 Female luer 피팅을설치하고, SPE 컬럼을연결한다. 훨씬더많은용량을위해서는완전한 In-Line 시스템을만들어야하며 SPE 컬럼 (22 쪽참조 ) 과 1/8 OD 의튜빙을위해적당한실린지아답터를사용해야한다. www.dongmyung.co.kr 5953 In-Line Filtration System with Benchtop Vacuum Station Vacuum Filtration Systems 1L Filter Funnel System 1 6409 Includes Base, Mesh Filter Support, Adaptor Kit, 1L Funnel 2L Filter Funnel System 1 6429 Includes Base, Mesh Filter Support, Adaptor Kit, 2L Funnel In-Line Filtration System 1 6407 Includes Base, Mesh Filter Support, Adaptor Kit, In-Line Filter Cap Replacement Parts 1L Funnel Only 1 6473 In-Line Filter Cap Only 1 6463 Replacement Mesh Filter Support Ring 1 6418 Replacement Adaptor Kit 1 6438 Stainless Steel Mesh Filter Support Ring 1 6421 4384

HPLC Certified Syringe Filters New and Improved Syringe Filters! 새로운 Luer-Lock Inlet 품질관리 (QC) 향상 재생하여사용할수있는용기 Alltech의실린지여과기는새로운모습과특성으로설계되었다. 모든실린지여과기는새로운포장용기인재생이가능한 Nalgene 4782 polypropylene 재질로되어있다. 이면에는가장인기있는실린지여과기목록이수록되어있으며좀더완벽한자료가필요하면 Alltech 종합카다록을참조하기바랍니다. Syringe Filter Specifications PTFE Syringe Filters PORE SIZE QTY PART NO. 4mm Syringe Filters 0.2μm 100 2394 1000 605043 0.45μm 100 2395 1000 605041 13mm Syringe Filters 0.2μm 100 2164 1000 605017 0.45μm 100 2165 1000 605015 25mm Syringe Filters 0.2μm 100 2089 1000 605710 0.45μm 100 2090 1000 605810 1.0μm 100 2429 Nylon: PVDF: PTFE: Regenerated Cellulose: Hydrophilic, good solvent resistance, moderate protein binding Hydrophilic, high solvent resistance, low protein binding Hydrophobic, very high solvent resistance, high protein binding Hydrophilic, high solvent resistance, very low protein binding Vacuum Filtration Systems PORE SIZE QTY PART NO. 4mm Syringe Filters 0.2μm 100 2091 1000 605039 0.45μm 100 2092 1000 605037 13mm Syringe Filters 0.2μm 100 2166 1000 605013 0.45μm 100 2167 1000 605011 25mm Syringe Filters 0.2μm 100 2045 1000 2945 0.45μm 100 2047 1000 605410 5.0μm 100 2940 25mm Syringe Filters with Glass 0.2μm Nylon w/glass 100 2357 0.45μm Nylon w/glass 100 2359 Regenerated Cellulose Syringe Filters PORE SIZE QTY PART NO. 4mm Syringe Filters 0.2μm 100 656100 1000 605055 0.45μm 100 656102 1000 605053 13mm Syringe Filters 0.2μm 100 656114 1000 605029 0.45μm 100 656116 1000 605027 25mm Syringe Filters 0.2μm 100 656124 0.45μm 100 656126 1000 605031 25mm Syringe Filters with Glass 0.2μm 100 656140 0.45μm 100 656141 PVDF Syringe Filters PORE SIZE QTY PART NO. 4mm Syringe Filters 0.2μm 100 2227 1000 605047 0.45μm 100 2228 1000 605045 Glass Fiber Only Syringe Filters MEMBRANE QTY PART NO. 25mm Syringe Filters 1.2μm 100 2347 13mm Syringe Filters 0.2μm 100 2647 1000 605021 0.45μm 100 2648 1000 605019 25mm Syringe Filters 0.2μm 100 2223 0.45μm 100 2224

Pre-Cut Filter Membranes Pre-Cut Membranes 6종의여과기규격을제공 다양한세공규격과멤브레인형태 비-멸균상태로공급 Anodisc Pre-Cut Membranes DIAMETER PORE SIZE QTY PART NO. 13mm (no support ring) 0.02μm 100 2935 13mm (no support ring) 0.1μm 100 2933 13mm (no support ring) 0.2μm 100 2253 25mm 0.02μm 50 2255 25mm 0.1μm 50 2268 25mm 0.2μm 50 2250 47mm 0.02μm 50 2235 47mm 0.1μm 50 2276 47mm 0.2μm 50 2230 Cellulose Acetate Pre-Cut Membranes Anodisc Membranes Anodisc membrane은친수성 (hydrophilic) 이며용매저항성이크다. 비록우리는진공시스템과함께멤브레인지지체를사용할것을추천하지만그러한것들은진공과압력시스템모두에서작동할것이다. 일반적인여과와수용성, 그리고유기시료의멸균을위해사용하면된다. 25mm와 47mm 여과기는 Polypropylene 지지체고리와함께사용해야한다. Cellulose Acetate Membranes Cellulose Acetate membrane은친수성 (hydrophilic) 이며매우낮은단백질머무름을보여준다. 일반적으로생물학시료의여과나멸균시에사용한다. Nylon Membranes Nylon membrane은친수성 (hydrophilic) 이고용매에대한저항성이크다. 수용성또는유기용액을여과하거나정화시사용한다. PTFE Membranes PTFE membrane은소수성 (hydrophobic) 이고큰용매저항성을갖는다. Air, 가스, 그리고비수용성용액을여과시에사용한다. 수용성용액과함께사용하기전에메탄올로전처리후사용한다. Regenerated Cellulose Membranes Regenerated Cellulose membrane은친수성 (hydrophilic) 이며높은용매저항성을갖는다. HPLC 시료를준비하거나일반적으로생물학시료를여과시에사용한다. Use 47mm filters with Vacuum Filter Systems, refer to page 25 or the Alltech Solvent Filtration Apparatus found in Catalog 500 page 541. Use 13 and 25mm filter membranes with the Filter Syringe Kit found in Catalog 500 page 254. Catalog pages are accessible on the internet at www.alltechweb.com. 4777 DIAMETER PORE SIZE QTY PART NO. 13mm 0.2μm 100 2125 13mm 0.45μm 100 2126 25mm 0.2μm 100 2128 25mm 0.45μm 100 2129 47mm 0.2μm 100 2133 47mm 0.45μm 100 2135 90mm 0.2μm 25 2573 90mm 0.45μm 25 2579 142mm 0.2μm 25 2574 142mm 0.45μm 25 2580 Nylon Pre-Cut Membranes DIAMETER PORE SIZE QTY PART NO. 9mm 0.2μm 100 8196 9mm 0.45μm 100 8198 13mm 0.2μm 100 2046 13mm 0.45μm 100 2044 25mm 0.2μm 100 2050 25mm 0.45μm 100 2048 47mm 0.2μm 100 2034 47mm 0.45μm 100 2024 90mm 0.2μm 25 2540 90mm 0.45μm 25 2546 PTFE Pre-Cut Membranes DIAMETER PORE SIZE QTY PART NO. 13mm 0.2μm 100 2013 13mm 0.45μm 100 2015 25mm 0.2μm 100 2023 25mm 0.45μm 100 2029 25mm 1.0μm 100 2056 47mm 0.2μm 100 2057 47mm 0.45μm 100 2058 47mm 1.0μm 100 2059 90mm 0.2μm 25 2552 90mm 0.45μm 25 2558 142mm 0.2μm 25 2553 142mm 0.45μm 25 2559 Regenerated Cellulose Pre-Cut Membranes DIAMETER PORE SIZE QTY PART NO. 47mm 0.2μm 100 204700 47mm 0.45μm 100 504700 www.dongmyung.co.kr 27

Mini UniPreps Mini-UniPreps는 4mm 여과기를대체할수있다! Polypropylene 재질의하우징 0.45μm 세공의다양한 Membrane 여과기 하나의장치로실린지필터와 Vial이함께하는획기적인신제품 Mini-UniPrep은여과기와자동시료주입기 (autosampler) vial이함께있어한동작으로빠르게시료를처리할수있다. 이장치는 Vial 쳄버와플런저의 2개부분으로구성되어있다. 플런저는바닥에하나의멤브레인여과기와상단에캡 / 셉텀이있다. 5903 Mini-UniPrep Nylon Mini-UniPrep 0.45μm 100 6802 1000 6804 PTFE Mini-UniPrep 0.45μm 100 6810 1000 6812 PVDF Mini-UniPrep 0.45μm 100 6818 1000 6820 사용방법은 Vial안으로시료을넣고플런저를끼운다. 그리고플런저에압력을가해 Vial의바닥까지시료를통과시키면시료는멤브레인여과기를통과하여플런저몸체의빈공간으로들어갈것이다. 12x32mm vial을가지고뚜껑을닫고자동시료주입기나다른기계에사용해보기바란다. 1-, 6-Position Compressor는다량의시료를처리할때, 손의피로를줄여준다. Mini-UniPrep은실린지여과기와 Vial를각각분리하여구매하는것보다매우경제적이며그러한것들은실험준비에필요한시간을획기적으로단축시켜준다. Mini-UniPrep Specifications Body: Polypropylene Septum: Teflon coated silicone rubber Septum Thickness: 1/400 Vial Size: 12 x 32mm Compressors 1-Position Compressor Ea 6822 6-Position Compressor Ea 6824 5954 Visit our new and improved website www.alltechweb.com